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Milk-Hormones: Narrative Review 牛奶-激素:叙事回顾
Pub Date : 2018-01-01 DOI: 10.4172/2329-888X.1000212
Archana Mourupoju, Vigneswaran Sundaresan
Milk is the major source of nourishment for newborn mammals prior to the other types of food. The composition of the cow’s milk widely varies between the different breeds and also during the different stages of lactation. Milk from animals contains fat, carbohydrates, vitamins, proteins, minerals and water. Water is the main constituent of milk which dilutes the milk and allowing its secretion from the body. Milk is the secretion of Mammary glands; hence it consists of constituents from the blood plasma, accordingly hormones that are transported in blood may be detectable in milk. The importance of hormones was discussed about decades back but still there are more concerns to look at the hormones as biomarkers in some serious problems and during pregnancy. In recent research, it has been found that further property of hormones in dairy items has conceivable effect on human wellbeing including the part of a few estrogens and insulin-like development factor which leads to bosom, prostate and endometrial tumors. Dairy animals' normally contains various steroid and protein (peptide) hormones in minute quantities. The most critical hormones found in milk and other dairy items which are identified by diagnostic techniques comprises of prolactin, steroids including estrogens, progesterone, corticoids, and androgens. Additionally, the presence of different hormones, for example, insulin-like development factor-1 (IGF-1) and neighborhood hormones including prostaglandins (PGs), in dairy items has been accounted for. It has been expected that a large portion of the hormones are moved into milk by dissemination. In this review we concentrated on several aspects of presence of hormones in dairy foods with especial emphasize on cow’s milk as a major source of consuming milk for humans.
牛奶是新生哺乳动物的主要营养来源,比其他类型的食物更重要。奶牛乳的成分在不同品种之间以及哺乳期的不同阶段差别很大。动物奶含有脂肪、碳水化合物、维生素、蛋白质、矿物质和水。水是牛奶的主要成分,它可以稀释牛奶并使其从体内分泌出来。乳汁是乳腺的分泌物;因此它由血浆成分组成,因此在血液中运输的激素可以在牛奶中检测到。激素的重要性在几十年前就已经讨论过了,但在一些严重问题和怀孕期间,人们仍然更关注将激素作为生物标志物。在最近的研究中,人们发现乳制品中激素的进一步特性对人类健康有可想而知的影响,包括一些雌激素和胰岛素样发育因子的一部分,导致乳房、前列腺和子宫内膜肿瘤。奶牛通常含有微量的各种类固醇和蛋白质(肽)激素。在牛奶和其他乳制品中发现的经诊断技术鉴定的最重要的激素包括催乳素、类固醇(包括雌激素、黄体酮、皮质激素和雄激素)。此外,乳制品中存在不同的激素,例如胰岛素样发育因子-1 (IGF-1)和邻近激素,包括前列腺素(pg)。据估计,大部分激素通过传播进入乳汁。在这篇综述中,我们集中在乳制品中激素存在的几个方面,特别强调奶牛的牛奶是人类消费牛奶的主要来源。
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引用次数: 2
Assessment of Sheep and Goat (Small Ruminants) Production System in Esera District, of Dawro Zone, Southern Ethiopia 埃塞俄比亚南部Dawro地区Esera地区绵羊和山羊(小型反刍动物)生产系统评估
Pub Date : 2018-01-01 DOI: 10.4172/2329-888X.1000215
A. Alilo, Abegaze Beyene, M. Mola
This study was conducted in Esera district Dawuro zone Southern Ethiopia with the objectives of assessing small ruminant production system. For the study one hundred thirty eight (HHs) owning small ruminant were selected randomly and semi-structured questionnaire was used to collect data on small ruminants’ production system, management, reproductive performances and availability of feed resources .The available feed resources are (a grass species, crop aftermath and 4 legumes species are the common ones). The average family size was 5.23 ± 0.195 per HH. Crop-livestock farming was the commonly used farming system (100%) with (69.1%) extensive and (30.9%) semi-intensive production system. The mean total land holding was 3.1288 ± 0.19 ha per HH and was significant (P<0.05) varied across agro-ecologies. The average sheep and goat flock size per HH was 6.08 ± 0.183 and 5.69 ± 0.236 respectively and was significantly (p<0.05) varied across agro-ecologies. The purpose of keeping small ruminants in District was for cash income, as an insurance, meat, manure and as means of wealth accumulation with index of 0.32, 0.29, 0.16, 0.13 and 0.08 respectively. Natural mating is the most widely used in their breeding practice almost (100%) in HL (High land), ML (Mid land), and LL (Low land). Grazing and browsing on natural pasture and leguminous tree species are commonly used in their feeding system. Majority (80.7%) of HHs are keeping small ruminants in their living house. The source of water used for their animals and themselves as well is from river, tap, rain and harvested water depending on the season. Internal and external parasites are the first and second ranking diseases and parasites which affects the small ruminants in the study area. For sheep, estimated average puberty age is (6.6 ± 0.12 months for male and 7.7 ± 0.16 months for female), age for first lambing (12.7 ± 0.16 month) and lambing interval (8.4 ± 0.17 month) were significantly Higher (P<0.05) in the HL than in ML and LL. For Goats, estimated average puberty age is (7.04 ± 0.10 months for male and 7.40 ± 0.10 months for female), first Kidding age is (13.04 ± 0.16 months) and lambing interval (8.5 ± 0.12 months) were significantly Higher (P<0.05) in the HL than in ML and LL agro ecology. The major opportunity of small ruminant production is that they requires short generation interval, high market demand smaller space and capital investment with index of 0.26, 0.24 and 0.23 respectively and the major constraints of small ruminant productions were Disease and parasite, feed shortage, insufficient grazing land were the major complaints told by our respondents.
本研究在埃塞俄比亚南部达乌罗地区的Esera区进行,目的是评估小型反刍动物生产系统。本研究随机选取138头小反刍动物饲养户,采用半结构化问卷调查法,对小反刍动物的生产体系、管理、繁殖性能和饲料资源利用情况进行了调查,其中有效饲料资源有1种禾本科动物,常见的有作物余粮和4种豆科动物。平均家庭人数为5.23±0.195人/ HH。农牧业是最常用的耕作方式(100%),其中粗放型(69.1%)和半集约型(30.9%)。平均土地持有总量为3.1288±0.19 ha / HH,不同农业生态类型间差异显著(P<0.05)。绵羊和山羊的平均每小时羊群数分别为6.08±0.183和5.69±0.236,不同农业生态区差异显著(p<0.05)。区内饲养小反刍动物的目的为获取现金收入、作为保险、作为肉类、作为粪肥、作为财富积累手段,其指数分别为0.32、0.29、0.16、0.13和0.08。自然交配在高陆、中陆和低地的繁殖实践中应用最为广泛(100%)。以天然牧草和豆科树种为主要食材。大多数(80.7%)的HHs在他们的生活中饲养小反刍动物。他们的动物和他们自己的水源是河流、自来水、雨水和收获的水,这取决于季节。内寄生虫和外寄生虫是影响研究区小反刍动物的前、第二位疾病和寄生虫。绵羊的平均青春期年龄(公羊6.6±0.12个月,母羊7.7±0.16个月),初产羔龄(12.7±0.16个月)和产羔间隔(8.4±0.17个月)在HL组显著高于ML组和LL组(P<0.05)。山羊的平均青春期年龄雄性为(7.04±0.10)个月,雌性为(7.40±0.10)个月,首次开玩笑年龄为(13.04±0.16)个月,产羔间隔为(8.5±0.12)个月(P<0.05)显著高于ML和LL农业生态山羊。小反刍动物生产的主要机遇是繁殖间隔短、市场需求大、占地面积小、资金投入少,指数分别为0.26、0.24和0.23;小反刍动物生产的主要制约因素是疾病和寄生虫、饲料短缺、牧场不足。
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引用次数: 13
Recent Trend in the Dairy Industry 乳制品行业的最新趋势
Pub Date : 2018-01-01 DOI: 10.4172/2329-888X.1000E134
Y. Park
Milk has been known as nature’s most complete food. The proof is that a newborn baby does not need any other foods except mother’s milk. However, the traditional and contemporary view of the role of milk has been remarkably expanded beyond the horizon of nutritional subsistence of infants. Milk is more than a source of nutrients to any neonate of mammalian species, as well as for growth of children and nourishment of adult humans [4].
牛奶被认为是自然界最完整的食物。证据是,一个新生婴儿不需要任何其他的食物,除了母乳。然而,传统和现代对牛奶的作用的看法已经大大超出了婴儿营养生存的范围。牛奶不仅是哺乳类新生动物的营养来源,也是儿童成长和成年人的营养来源。
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引用次数: 6
Thermosonication as an Upcoming Technology in the Dairy Industry: An Overview 热硫化作为乳制品行业的一项新兴技术:综述
Pub Date : 2017-09-22 DOI: 10.4172/2329-888X.1000189
Sun, ita Ghosh
Ultrasound waves are longitudinal sound waves of frequency of 20 kHz or more. As low amplitude ultrasonic waves do not significantly modify the material under examination, they can be used to analyse the material. However, high amplitude ultrasonic waves can be used to process food as they have the capacity to alter the food material by cavitation. The use of ultrasound by itself may not be effective in destroying all the microbes in food. Hence, it can be coupled with pressure or heat or both to get the desired results. Thermosonication involves the simultaneous use of low frequency ultrasound waves (20 kHz) along with heat; and the both together have some synergistic effect. When heat and ultrasound is used together, the process temperature is considerably reduced compared to the conventional heating process, making it a green and economical technology as less energy is consumed; this in turn makes it a cost-efficient process. If thermosonication is seen to bring about the desired effects in milk, then it can be used as a commercial method to treat and homogenize milk in the future.
超声波是频率在20khz或更高的纵向声波。由于低振幅超声波不会显著改变被检查的材料,它们可以用来分析材料。然而,高振幅超声波可用于加工食品,因为它们具有通过空化改变食品材料的能力。使用超声波本身可能不能有效地消灭食物中的所有微生物。因此,它可以与压力或热量或两者相结合,以获得所需的结果。热超声包括同时使用低频超声波(20千赫)和热量;两者共同具有一定的协同效应。当热量和超声波同时使用时,与传统的加热过程相比,过程温度大大降低,使其成为一种绿色经济的技术,因为消耗的能源更少;这反过来又使它成为一个成本效益高的过程。如果热超声可以在牛奶中产生预期的效果,那么它可以在未来作为一种商业方法来处理和均质牛奶。
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引用次数: 7
Adjunctive Application of Solid-State Culture Products from Aspergillus Oryzae for Semi-Hard Cheese 米曲霉固态培养物在半硬质干酪中的辅助应用
Pub Date : 2017-09-12 DOI: 10.4172/2329-888X.1000188
H. Kumura, C. Saito, Y. Taniguchi, Taiki Machiya, Yutaro Takahashi, Ken Kobayashi, A. Kimura
To develop a novel type of cheese using Aspergillus oryzae, four strains (AHU 7139, AHU 7140, AHU 7141 and AHU 7146) were tested with respect to their extracellular protease production on solid-state media containing heatdenatured whey protein under various temperatures and pH levels. Proteolytic activity of the crude enzyme extracts was evaluated under acidic condition considering the circumstance of cheese ripening. Zymographic analysis revealed that several kinds of proteases were secreted, which were dependent on the strain, incubation temperature and initial pH value. Using three strains, six types of culture products were selected as adjunctive materials in combination with an incubation temperature of 15 or 20oC and an initial pH condition of 4.0 or 6.5. Gouda-type cheese curd was prepared, and the curd particle received 1% (w/w) of the culture product, followed by pressing, cooling, salting and ripening for 3 months at 11.5oC. An increase in water extractable nitrogen and free fatty acid was observed in some experimental cheeses, depending on the strain and culture history. In particular, incorporation of culture products from A. oryzae AHU 7139, incubated at pH 6.5 and 20oC affected these parameters. As a novel dairy technology, this study propose new insights into the potential use of A. oryzae for providing an enzyme cocktail to develop palatable cheese flavor when suitable strains and culture conditions offer products with an optimum balance of proteases and lipases.
为了利用米曲霉开发新型奶酪,在不同温度和pH水平下,测试了四个菌株(AHU 7139、AHU 7140、AHU 7141和AHU 7146)在含有热变性乳清蛋白的固态培养基上的细胞外蛋白酶生产。考虑干酪成熟的环境,在酸性条件下评价了粗酶提取物的蛋白酶活性。酶谱分析显示,分泌了几种蛋白酶,这取决于菌株、培养温度和初始pH值。使用三种菌株,选择六种类型的培养产物作为辅助材料,结合15或20℃的培养温度和4.0或6.5的初始pH条件。制备了Gouda型干酪凝乳,凝乳颗粒接受1%(w/w)的培养产物,然后在11.5℃下压制、冷却、腌制和成熟3个月。根据菌株和培养历史,在一些实验干酪中观察到水可提取氮和游离脂肪酸的增加。特别是,加入在pH 6.5和20℃下培养的米曲霉AHU 7139的培养产物会影响这些参数。作为一种新的乳制品技术,本研究对米曲霉提供酶混合物的潜在用途提出了新的见解,当合适的菌株和培养条件为产品提供蛋白酶和脂肪酶的最佳平衡时,米曲霉可以产生美味的奶酪风味。
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引用次数: 5
Effect of Sodium Chloride and Incision on the Chicken Pepsin Coagulant Activity Extracted from Proventriculus, Dried Under Partial Vacuum 氯化钠和切口对部分真空干燥鸡前胃肽凝固剂活性的影响
Pub Date : 2017-09-06 DOI: 10.4172/2329-888X.1000187
Benyahia-Krid Férial Aziza, Adoui Faïza, Aissaoui-Zitoun Ouarda, Boughellout Halima, Siar El-Hocine, Zikiou Abdellah, Zidoune Mohammed Nasereddine
Rennet covers only 30% of the world's cheese production because the availability of calf stomach becomes limited (FAO). This lack has suggested the search for animal, vegetable or microbial enzyme substitutes. Among the alternative animal enzymes, chicken pepsin. In order to study the chicken pepsin stability over time, chicken proventriculus, whole or incised into four parts or incised into slices, without or with salt addition distributed into six different lots, were dried under partial vacuum (47°C, 800 mbar). The effects of the incision or not, as well as the addition or not of salt, and storage time of dried proventriculus, on the coagulant activity of pepsin extracts (expressed in equivalents Rennet Units) were studied. The six lots pepsin residual activities determined immediately after drying operation expressed the relative yield in the fresh state before storage. They were between 50% for proventriculus cut into four parts without salt addition and 18% for proventriculus with salt addition. After 54 days of storage, the residual activity was relatively distinct for proventriculus cut into four parts without salt addition: 35.5% and for the proventriculus incised into slices with salt addition of 4.7%. Salt seemed to have caused a great loss of activity during salting. In addition, the incision effect combined to salt addition showed a remarkable loss of activity. During the storage period, the pepsin residual coagulant activity showed better stability of partially vacuum-dried proventriculus cut into four parts and unsalted.
Rennet只覆盖了世界奶酪产量的30%,因为小牛胃的供应有限(粮农组织)。这种缺乏表明需要寻找动物、蔬菜或微生物的酶替代品。在替代动物酶中,鸡胃蛋白酶。为了研究鸡胃蛋白酶随时间的稳定性,在部分真空(47°C,800毫巴)下干燥鸡前胃,将鸡前胃全部或切成四部分或切成片,不加盐或加盐,分为六个不同批次。研究了切口与否、盐的添加与否、干燥前胃的储存时间对胃蛋白酶提取物(以等效Rennet单位表示)的凝血活性的影响。干燥操作后立即测定的六批胃蛋白酶残留活性表示储存前新鲜状态下的相对产率。在不加盐的情况下,将前胃切成四部分的比例在50%之间,在加盐的前胃的比例在18%之间。贮藏54d后,不加盐切四部分的前胃和加盐4.7%的切片的前胃的剩余活性相对明显:35.5%。盐似乎在腌制过程中造成了很大的活性损失。此外,切口效应与加盐相结合显示出显著的活性损失。在贮藏期间,部分真空干燥的四份无盐前胃的胃蛋白酶残留混凝剂活性表现出较好的稳定性。
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引用次数: 2
Alginate Oligomers Inhibit Growth of Bacteria Causing Bovine Mastitis and Potentiate the Activity of Antibiotics Commonly Used For Treatment of the Disease 海藻酸酯低聚物抑制引起牛乳腺炎的细菌的生长并增强通常用于治疗该疾病的抗生素的活性
Pub Date : 2017-09-01 DOI: 10.4172/2329-888X.1000186
A. Tøndervik, G. Klinkenberg, I. Aune, P. Rye, H. Sletta
Alginate oligomers have been shown to disrupt microbial biofilms and reduce minimum inhibitory concentrations (MIC) for many clinically relevant antibiotics to a range of human pathogens. The antibiofilm and potentiating effects of alginate oligomers has previously been focussed on bacterial and fungal strains that play significant roles in chronic human infections. This study on the effects of an alginate oligomer (OligoG CF-5/20) on bacteria that cause bovine mastitis is the first report that investigates a significant disease in animal health. The effect of OligoG CF-5/20 on the inhibitory concentrations of ampicillin, erythromycin, cephalothin and lincomycin to a test panel of nine bacterial strains associated with bovine mastitis were investigated. Two different media were used for cultivation, the standard Mueller-Hinton normally used for bacterial MIC determination, and a skimmed milk medium to mimic the native growth conditions for mastitis bacteria. OligoG CF-5/20 was shown to inhibit growth of all strains tested, and demonstrated a 2 to 8 fold reduction in MICs for erythromycin, cephalothin and lincomycin. The data highlights a potential role for alginate oligomers in potentiating the efficacy of antibiotics, and thereby potentially reducing antibiotic use, in the treatment of mastitis in dairy cattle.
海藻酸寡聚物已被证明可以破坏微生物生物膜,并降低许多临床相关抗生素对一系列人类病原体的最低抑制浓度(MIC)。藻酸盐低聚物的抗生物膜和增强作用以前一直集中在细菌和真菌菌株上,这些菌株在慢性人类感染中发挥着重要作用。这项关于海藻酸盐低聚物(OligoG CF-5/20)对引起乳腺炎的细菌的影响的研究是第一份研究动物健康中一种重要疾病的报告。研究了OligoG CF-5/20对氨苄青霉素、红霉素、头孢噻吩和林可霉素对9株乳腺炎相关菌株的抑制浓度的影响。使用两种不同的培养基进行培养,通常用于细菌MIC测定的标准Mueller-Hinton培养基和模拟乳腺炎细菌天然生长条件的脱脂乳培养基。OligoG CF-5/20显示可抑制所有测试菌株的生长,并显示红霉素、头孢噻吩和林可霉素的MIC降低2至8倍。该数据强调了海藻酸盐低聚物在提高抗生素疗效方面的潜在作用,从而有可能减少奶牛乳腺炎的抗生素使用。
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引用次数: 1
Factors Affecting Sensory Quality of Goat Milk Cheeses: A Review 影响羊奶干酪感官品质的因素综述
Pub Date : 2017-08-31 DOI: 10.4172/2329-888X.1000185
Y. Park, Chelsea Jeanjulien, Aftab Siddique
The combination of tastes and aromas associated with caprine milk lead to a range of unique flavors and sensory properties that are critical to overall quality of caprine milk and its processed products. Goat milk production has risen dramatically in the past two decades. Since numerous breeds of goats have been raised under different localities, environmental and management conditions in many parts of the world, no single factor can universally describe or identify sensory characteristics of a wide variety of goat cheeses produced and consumed around the world. Rather, there are many factors that influence the flavor and sensory qualities of goat milk cheeses. The flavor of cheese arises from a series of complex reactions involving microbial metabolism and enzymatic reactions which include proteolysis of proteins, lipolysis of fats, and fermentation of carbohydrates. Caprine milk reportedly has more fat, protein and ash, and less lactose than cow milk. The types of pasture fed to an animal not only affect the taste of cheese but the color of the products. Because of the amount of carotene present in large amounts in green forage and its contribution to the yellow color in cheese, cheeses made with spring milk are likely to be more yellowish color than those made with winter milk. Lipolysis of fat causes rancid flavor defect in milk, cheese, and other dairy products, while proteolysis provides a major impact on flavor, aroma, and textural characteristics of most cheese varieties. Much more research needs to be done on the sensory quality of goat milk cheese to show the factors directly causing sensory properties of different varieties of goat milk cheeses. Understanding the complexity of flavors embodied by goat milk and goat milk products further aids in the production of quality products that enhance the consumer acceptability of caprine milk cheese products. This paper explores the current up-to-date reports on various factors associated with sensory qualities of caprine milk cheese products.
与山羊奶相关的口味和香气的结合产生了一系列独特的风味和感官特性,这些特性对山羊奶及其加工产品的整体质量至关重要。在过去的二十年里,羊奶产量急剧上升。由于世界许多地区在不同的地点、环境和管理条件下饲养了许多山羊品种,因此没有一个单一的因素能够普遍描述或识别世界各地生产和消费的各种山羊奶酪的感官特征。相反,影响羊奶奶酪风味和感官品质的因素有很多。奶酪的风味来源于一系列复杂的反应,包括微生物代谢和酶促反应,包括蛋白质的蛋白水解、脂肪的脂解和碳水化合物的发酵。据报道,羊奶比牛奶含有更多的脂肪、蛋白质和灰分,乳糖更少。喂养动物的牧场类型不仅会影响奶酪的味道,还会影响产品的颜色。由于绿色饲料中含有大量的胡萝卜素,而且胡萝卜素会使奶酪呈现黄色,因此用春季牛奶制成的奶酪可能比用冬季牛奶制成的更呈黄色。脂肪的脂解会导致牛奶、奶酪和其他乳制品的酸败风味缺陷,而蛋白水解会对大多数奶酪品种的风味、香气和质地特征产生重大影响。山羊奶干酪的感官品质有待进一步研究,以揭示不同品种山羊奶干酪感官品质的直接影响因素。了解羊奶和羊奶产品所体现的风味的复杂性,有助于生产高质量的产品,提高消费者对山羊奶奶酪产品的可接受性。本文探讨了与山羊奶干酪产品感官品质相关的各种因素的最新报道。
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引用次数: 17
Prevalence of Bovine Clinical Mastitis and Farmer’s Awareness in and Around Wolaita Sodo, Southern Ethiopia 埃塞俄比亚南部Wolaita Sodo及其周围地区牛临床乳腺炎的患病率和农民的意识
Pub Date : 2017-08-26 DOI: 10.4172/2329-888X.1000184
F. Abraham, efrot Meaza Zeleke
A cross sectional study was conducted to determine prevalence and awareness of farm owners from November, 2016 to April, 2017 in randomly selected dairy farms in and around Wolaita Sodo town. A total of 155 lactating cows of three breeds (local, Local-Holstein Frisian cross and exotic (Jersey) from twenty five dairy farms were considered for the study. The overall prevalence of bovine clinical mastitis was 5.1%. Among the variables included in the study only lactation stage and presence of teat end lesion had significant association with prevalence of bovine clinical mastitis (P<0.05). The questionnaire based interview of smallholder dairy farm owners/attendants about awareness of bovine mastitis showed serious gap about awareness on effect, clinical manifestations and subclinical mastitis. Hence, it is recommended to implement strategic extension program to control and prevention of the disease and the smallholder dairy farmers need awareness training to enhance production and productivity of productive life of the cow.
2016年11月至2017年4月,在Wolaita Sodo镇及其周围随机选择的奶牛场进行了一项横断面研究,以确定农场主的患病率和意识。本研究共考虑了来自25个奶牛场的三个品种(本地、本地荷斯坦-弗里斯兰杂交和外来(泽西))的155头泌乳奶牛。牛临床乳腺炎的总体患病率为5.1%。在纳入研究的变量中,只有哺乳期和乳头末端病变的存在与牛临床乳房炎的患病率显著相关(P<0.05)。基于问卷的小型奶牛场老板/服务员对牛乳腺炎意识的访谈显示,在效果意识方面存在严重差距,临床表现和亚临床乳腺炎。因此,建议实施战略推广计划,以控制和预防该疾病,小农户奶农需要意识培训,以提高奶牛的生产和生产生活生产力。
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引用次数: 5
Characterization of Silter Cheeses Produced in Valley and Alpine Pastures by a Proteomic Approach 用蛋白质组学方法表征山谷和高山牧场生产的银色奶酪
Pub Date : 2017-08-04 DOI: 10.4172/2329-888X.1000183
G. Rusco, A. Natale, C. Palermo, S. Chessa, D. Nardiello, A. Caroli, D. Centonze, A. Luccia, B. Gatta
Silter is an Italian hard cheese manufactured with milk produced by cows fed at different altitude, valley or alpine pasture. The chemical, rheological and sensory properties of cheeses can be affected by the modification in milk composition due to the breed which at different altitude causes the modification of protein content, κ-CN glycosylation, plasmin activity, and coagulation properties. The influence of milk plasmin activity on dairy production was investigated in seven Silter cheeses, four produced in the valley and three from alpine mountain through alkaline urea-polyacrylamide gel electrophoresis; two-dimensional gel electrophoresis coupled to mass spectrometry and image analysis. Results demonstrated that Silter cheese obtained from cows reared in alpine pasture is characterized by a more evident proteolysis, determining high levels of β-CN and αs1-CN fragments. Therefore, the most relevant fragmentation was attributed to a more intense activity of plasmin and to a different dosage of rennet to make up for the reduced coagulation properties of alpine milk.
Silter是一种意大利硬奶酪,由在不同海拔、山谷或高山牧场饲养的奶牛生产的牛奶制成。奶酪的化学、流变和感官特性可能会受到牛奶成分变化的影响,因为不同海拔高度的品种会导致蛋白质含量、κ-CN糖基化、纤溶酶活性和凝血特性的变化。采用碱性尿素-聚丙烯酰胺凝胶电泳法,研究了7种粉质干酪、4种产自山谷干酪和3种产自高山干酪的乳纤溶酶活性对乳制品生产的影响;二维凝胶电泳结合质谱和图像分析。结果表明,从高山牧场饲养的奶牛身上获得的粉质干酪具有更明显的蛋白水解特征,确定了高水平的β-CN和αs1-CN片段。因此,最相关的碎片化归因于更强烈的纤溶酶活性和不同剂量的凝乳酶,以弥补高山牛奶凝结特性的降低。
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引用次数: 2
期刊
Journal of advances in dairy research
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