Pub Date : 2024-07-11DOI: 10.1007/s12551-024-01207-4
Kelly M. Thayer, Sean Stetson, Fernando Caballero, Christopher Chiu, I. Han
{"title":"Navigating the complexity of p53-DNA binding: implications for cancer therapy","authors":"Kelly M. Thayer, Sean Stetson, Fernando Caballero, Christopher Chiu, I. Han","doi":"10.1007/s12551-024-01207-4","DOIUrl":"https://doi.org/10.1007/s12551-024-01207-4","url":null,"abstract":"","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141657151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-09DOI: 10.1007/s12551-024-01208-3
R. D. Peluffo, Silvia del V. Alonso, R. Itri, F. L. G. Flecha, Leandro R S Barbosa
{"title":"LAFeBS, alive, kicking, and growing: the story continues…","authors":"R. D. Peluffo, Silvia del V. Alonso, R. Itri, F. L. G. Flecha, Leandro R S Barbosa","doi":"10.1007/s12551-024-01208-3","DOIUrl":"https://doi.org/10.1007/s12551-024-01208-3","url":null,"abstract":"","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141663823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-04DOI: 10.1007/s12551-024-01205-6
Andrea Orsetti, D. van Oosten, Roxana-Geanina Vasarhelyi, Theodor-Marian Dănescu, Jan Huertas, Hugo van Ingen, Vlad Cojocaru
{"title":"Structural dynamics in chromatin unraveling by pioneer transcription factors","authors":"Andrea Orsetti, D. van Oosten, Roxana-Geanina Vasarhelyi, Theodor-Marian Dănescu, Jan Huertas, Hugo van Ingen, Vlad Cojocaru","doi":"10.1007/s12551-024-01205-6","DOIUrl":"https://doi.org/10.1007/s12551-024-01205-6","url":null,"abstract":"","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141678699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-03eCollection Date: 2024-06-01DOI: 10.1007/s12551-024-01204-7
John H Maddocks, Pablo D Dans, Thomas H Cheatham, Sarah Harris, Charles Laughton, Modesto Orozco, Lois Pollack, Wilma K Olson
This editorial for Volume 16, Issue 3 of Biophysical Reviews highlights the three-dimensional structural and dynamic information encoded in DNA sequences and introduces the topics covered in this special issue of the journal on Multiscale Simulations of DNA from Electrons to Nucleosomes. Biophysical Reviews is the official journal of the International Union for Pure and Applied Biophysics (IUPAB 2024). The international scope of the articles in the issue exemplifies the goals of IUPAB to organize worldwide advancements, co-operation, communication, and education in biophysics.
生物物理评论》(Biophysical Reviews)第 16 卷第 3 期的这篇社论强调了 DNA 序列中编码的三维结构和动态信息,并介绍了本期特刊 "从电子到核小体的 DNA 多尺度模拟 "所涵盖的主题。生物物理评论》是国际纯粹与应用生物物理学联合会(IUPAB 2024)的官方期刊。本期文章的国际性体现了国际纯粹与应用生物物理学联合会组织全球生物物理学进步、合作、交流和教育的目标。
{"title":"Special issue: Multiscale simulations of DNA from electrons to nucleosomes.","authors":"John H Maddocks, Pablo D Dans, Thomas H Cheatham, Sarah Harris, Charles Laughton, Modesto Orozco, Lois Pollack, Wilma K Olson","doi":"10.1007/s12551-024-01204-7","DOIUrl":"10.1007/s12551-024-01204-7","url":null,"abstract":"<p><p>This editorial for Volume 16, Issue 3 of <i>Biophysical Reviews</i> highlights the three-dimensional structural and dynamic information encoded in DNA sequences and introduces the topics covered in this special issue of the journal on Multiscale Simulations of DNA from Electrons to Nucleosomes. <i>Biophysical Reviews</i> is the official journal of the International Union for Pure and Applied Biophysics (IUPAB 2024). The international scope of the articles in the issue exemplifies the goals of IUPAB to organize worldwide advancements, co-operation, communication, and education in biophysics.</p>","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11296990/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-18eCollection Date: 2024-06-01DOI: 10.1007/s12551-024-01198-2
Wilma K Olson, Robert T Young, Luke Czapla
DNA carries more than the list of biochemical instructions that drive the basic functions of living systems. The sequence of base pairs includes a multitude of structural and energetic signals that determine the degree to which the long, threadlike molecule moves and how it responds to proteins and other molecules involved in its processing and packaging. The arrangements of successive base pairs in high-resolution protein-DNA crystal structures provide useful benchmarks for atomic-level simulations of double-helical DNA as well as information potentially useful in interpreting the properties of specific DNA sequences. The set of currently available structures has enough examples to characterize the conformational preferences of the DNA base-pair steps within the context of their immediate neighbors, i.e., in the context of tetramers, and reveals surprising effects of certain neighbors on local chain properties. The proteins in contact with DNA present various microenvironments that sense and/or induce the observed spatial forms. The cumulative buildup of amino-acid atoms in different protein-DNA complexes produces a binding cloud around the double helix with subtle sequence-dependent features. While the microenvironment presented by each protein to DNA is highly unique, the overall composition of amino-acid atoms within close range of DNA in a broad collection of structures is fairly uniform. The buildup of protein atoms of different types around the DNA provides new information for the improvement of nucleic acid force fields and fresh ideas for the exploration of the properties of DNA in solution.
DNA 所承载的不仅仅是驱动生命系统基本功能的一系列生化指令。碱基对序列包含了大量的结构和能量信号,这些信号决定了线状长分子的运动程度,以及它如何对参与其加工和包装的蛋白质和其他分子做出反应。高分辨率蛋白质-DNA 晶体结构中连续碱基对的排列为双螺旋 DNA 的原子级模拟提供了有用的基准,也为解释特定 DNA 序列的特性提供了潜在的有用信息。目前可用的一组结构有足够多的实例来描述 DNA 碱基对步骤在其近邻(即四聚体)范围内的构象偏好,并揭示了某些近邻对局部链特性的惊人影响。与 DNA 接触的蛋白质呈现出各种微环境,这些微环境能够感知和/或诱导观察到的空间形式。不同蛋白质-DNA 复合物中氨基酸原子的累积形成了双螺旋周围的结合云,这种结合云具有微妙的序列依赖性特征。虽然每种蛋白质与 DNA 之间的微环境都非常独特,但在广泛的结构集合中,DNA 近距离内氨基酸原子的整体组成却相当一致。DNA 周围不同类型蛋白质原子的堆积为改进核酸力场提供了新的信息,也为探索 DNA 在溶液中的特性提供了新的思路。
{"title":"DNA simulation benchmarks revealed with the accumulation of high-resolution structures.","authors":"Wilma K Olson, Robert T Young, Luke Czapla","doi":"10.1007/s12551-024-01198-2","DOIUrl":"10.1007/s12551-024-01198-2","url":null,"abstract":"<p><p>DNA carries more than the list of biochemical instructions that drive the basic functions of living systems. The sequence of base pairs includes a multitude of structural and energetic signals that determine the degree to which the long, threadlike molecule moves and how it responds to proteins and other molecules involved in its processing and packaging. The arrangements of successive base pairs in high-resolution protein-DNA crystal structures provide useful benchmarks for atomic-level simulations of double-helical DNA as well as information potentially useful in interpreting the properties of specific DNA sequences. The set of currently available structures has enough examples to characterize the conformational preferences of the DNA base-pair steps within the context of their immediate neighbors, i.e., in the context of tetramers, and reveals surprising effects of certain neighbors on local chain properties. The proteins in contact with DNA present various microenvironments that sense and/or induce the observed spatial forms. The cumulative buildup of amino-acid atoms in different protein-DNA complexes produces a binding cloud around the double helix with subtle sequence-dependent features. While the microenvironment presented by each protein to DNA is highly unique, the overall composition of amino-acid atoms within close range of DNA in a broad collection of structures is fairly uniform. The buildup of protein atoms of different types around the DNA provides new information for the improvement of nucleic acid force fields and fresh ideas for the exploration of the properties of DNA in solution.</p>","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11297015/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-17eCollection Date: 2024-06-01DOI: 10.1007/s12551-024-01202-9
Konstantin Röder, Samuela Pasquali
Predicting the structure and dynamics of RNA molecules still proves challenging because of the relative scarcity of experimental RNA structures on which to train models and the very sensitive nature of RNA towards its environment. In the last decade, several atomistic force fields specifically designed for RNA have been proposed and are commonly used for simulations. However, it is not necessarily clear which force field is the most suitable for a given RNA molecule. In this contribution, we propose the use of the computational energy landscape framework to explore the energy landscape of RNA systems as it can bring complementary information to the more standard approaches of enhanced sampling simulations based on molecular dynamics. We apply the EL framework to the study of a small RNA pseudoknot, the Aquifex aeolicus tmRNA pseudoknot PK1, and we compare the results of five different RNA force fields currently available in the AMBER simulation software, in implicit solvent. With this computational approach, we can not only compare the predicted 'native' states for the different force fields, but the method enables us to study metastable states as well. As a result, our comparison not only looks at structural features of low energy folded structures, but provides insight into folding pathways and higher energy excited states, opening to the possibility of assessing the validity of force fields also based on kinetics and experiments providing information on metastable and unfolded states.
Supplementary information: The online version contains supplementary material available at 10.1007/s12551-024-01202-9.
{"title":"Assessing RNA atomistic force fields via energy landscape explorations in implicit solvent.","authors":"Konstantin Röder, Samuela Pasquali","doi":"10.1007/s12551-024-01202-9","DOIUrl":"10.1007/s12551-024-01202-9","url":null,"abstract":"<p><p>Predicting the structure and dynamics of RNA molecules still proves challenging because of the relative scarcity of experimental RNA structures on which to train models and the very sensitive nature of RNA towards its environment. In the last decade, several atomistic force fields specifically designed for RNA have been proposed and are commonly used for simulations. However, it is not necessarily clear which force field is the most suitable for a given RNA molecule. In this contribution, we propose the use of the computational energy landscape framework to explore the energy landscape of RNA systems as it can bring complementary information to the more standard approaches of enhanced sampling simulations based on molecular dynamics. We apply the EL framework to the study of a small RNA pseudoknot, the <i>Aquifex aeolicus</i> tmRNA pseudoknot PK1, and we compare the results of five different RNA force fields currently available in the AMBER simulation software, in implicit solvent. With this computational approach, we can not only compare the predicted 'native' states for the different force fields, but the method enables us to study metastable states as well. As a result, our comparison not only looks at structural features of low energy folded structures, but provides insight into folding pathways and higher energy excited states, opening to the possibility of assessing the validity of force fields also based on kinetics and experiments providing information on metastable and unfolded states.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12551-024-01202-9.</p>","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11297004/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-02eCollection Date: 2024-04-01DOI: 10.1007/s12551-024-01191-9
Damien Hall, Wilma K Olson
This Editorial for Volume 16 Issue 2 first describes the issue contents before describing some upcoming events within Biophysical Reviews and concludies with an announcement on the transition of Chief Editors thanks to the outgoing Chief Editor.
{"title":"<i>Biophysical Reviews</i>: a transition in the journal.","authors":"Damien Hall, Wilma K Olson","doi":"10.1007/s12551-024-01191-9","DOIUrl":"10.1007/s12551-024-01191-9","url":null,"abstract":"<p><p>This Editorial for Volume 16 Issue 2 first describes the issue contents before describing some upcoming events within <i>Biophysical Reviews</i> and concludies with an announcement on the transition of Chief Editors thanks to the outgoing Chief Editor.</p>","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11078897/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140911172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-30eCollection Date: 2024-04-01DOI: 10.1007/s12551-024-01186-6
Boris Semin, Aleksey Loktyushkin, Elena Lovyagina
Water oxidation in photosystem II (PSII) is performed by the oxygen-evolving complex Mn4CaO5 which can be extracted from PSII and then reconstructed using exogenous cations Mn(II) and Ca2+. The binding efficiency of other cations to the Mn-binding sites in Mn-depleted PSII was investigated without any positive results. At the same time, a study of the Fe cations interaction with Mn-binding sites showed that it binds at a level comparable with the binding of Mn cations. Binding of Fe(II) cations first requires its light-dependent oxidation. In general, the interaction of Fe(II) with Mn-depleted PSII has a number of features similar to the two-quantum model of photoactivation of the complex with the release of oxygen. Interestingly, incubation of Ca-depleted PSII with Fe(II) cations under certain conditions is accompanied by the formation of a chimeric cluster Mn/Fe in the oxygen-evolving complex. PSII with the cluster 2Mn2Fe was found to be capable of water oxidation, but only to the H2O2 intermediate. However, the cluster 3Mn1Fe can oxidize water to O2 with an efficiency about 25% of the original in the absence of extrinsic proteins PsbQ and PsbP. In the presence of these proteins, the efficiency of O2 evolution can reach 80% of the original when adding exogenous Ca2+. In this review, we summarized information on the formation of chimeric Mn-Fe clusters in the oxygen-evolving complex. The data cited may be useful for detailing the mechanism of water oxidation.
{"title":"Current analysis of cations substitution in the oxygen-evolving complex of photosystem II.","authors":"Boris Semin, Aleksey Loktyushkin, Elena Lovyagina","doi":"10.1007/s12551-024-01186-6","DOIUrl":"10.1007/s12551-024-01186-6","url":null,"abstract":"<p><p>Water oxidation in photosystem II (PSII) is performed by the oxygen-evolving complex Mn<sub>4</sub>CaO<sub>5</sub> which can be extracted from PSII and then reconstructed using exogenous cations Mn(II) and Ca<sup>2+</sup>. The binding efficiency of other cations to the Mn-binding sites in Mn-depleted PSII was investigated without any positive results. At the same time, a study of the Fe cations interaction with Mn-binding sites showed that it binds at a level comparable with the binding of Mn cations. Binding of Fe(II) cations first requires its light-dependent oxidation. In general, the interaction of Fe(II) with Mn-depleted PSII has a number of features similar to the two-quantum model of photoactivation of the complex with the release of oxygen. Interestingly, incubation of Ca-depleted PSII with Fe(II) cations under certain conditions is accompanied by the formation of a chimeric cluster Mn/Fe in the oxygen-evolving complex. PSII with the cluster 2Mn2Fe was found to be capable of water oxidation, but only to the H<sub>2</sub>O<sub>2</sub> intermediate. However, the cluster 3Mn1Fe can oxidize water to O<sub>2</sub> with an efficiency about 25% of the original in the absence of extrinsic proteins PsbQ and PsbP. In the presence of these proteins, the efficiency of O<sub>2</sub> evolution can reach 80% of the original when adding exogenous Ca<sup>2+</sup>. In this review, we summarized information on the formation of chimeric Mn-Fe clusters in the oxygen-evolving complex. The data cited may be useful for detailing the mechanism of water oxidation.</p>","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11078907/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140911173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-19eCollection Date: 2024-04-01DOI: 10.1007/s12551-024-01185-7
Chenyu Jin, Anupam Sengupta
Microbes thrive in diverse porous environments-from soil and riverbeds to human lungs and cancer tissues-spanning multiple scales and conditions. Short- to long-term fluctuations in local factors induce spatio-temporal heterogeneities, often leading to physiologically stressful settings. How microbes respond and adapt to such biophysical constraints is an active field of research where considerable insight has been gained over the last decades. With a focus on bacteria, here we review recent advances in self-organization and dispersal in inorganic and organic porous settings, highlighting the role of active interactions and feedback that mediates microbial survival and fitness. We discuss open questions and opportunities for using integrative approaches to advance our understanding of the biophysical strategies which microbes employ at various scales to make porous settings habitable.
{"title":"Microbes in porous environments: from active interactions to emergent feedback.","authors":"Chenyu Jin, Anupam Sengupta","doi":"10.1007/s12551-024-01185-7","DOIUrl":"10.1007/s12551-024-01185-7","url":null,"abstract":"<p><p>Microbes thrive in diverse porous environments-from soil and riverbeds to human lungs and cancer tissues-spanning multiple scales and conditions. Short- to long-term fluctuations in local factors induce spatio-temporal heterogeneities, often leading to physiologically stressful settings. How microbes respond and adapt to such biophysical constraints is an active field of research where considerable insight has been gained over the last decades. With a focus on bacteria, here we review recent advances in self-organization and dispersal in inorganic and organic porous settings, highlighting the role of active interactions and feedback that mediates microbial survival and fitness. We discuss open questions and opportunities for using integrative approaches to advance our understanding of the biophysical strategies which microbes employ at various scales to make porous settings habitable.</p>","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11078916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140911174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-12eCollection Date: 2024-06-01DOI: 10.1007/s12551-024-01183-9
Natacha Gillet, Elise Dumont, Emmanuelle Bignon
Cellular DNA is constantly exposed to endogenous or exogenous factors that can induce lesions. Several types of lesions have been described that can result from UV/ionizing irradiations, oxidative stress, or free radicals, among others. In order to overcome the deleterious effects of such damages, i.e., mutagenicity or cytotoxicity, cells possess a highly complex DNA repair machinery, involving repair enzymes targeting specific types of lesions through dedicated cellular pathways. In addition, DNA is highly compacted in the nucleus, the first level of compaction consisting of ~ 147 DNA base pairs wrapped around a core of histones, the so-called nucleosome core particle. In this complex environment, the DNA structure is highly constrained, and fine-tuned mechanisms involving remodeling processes are required to expose the DNA to repair enzymes and to facilitate the damage removal. However, these nucleosome-specific mechanisms remain poorly understood, and computational methods emerged only recently as powerful tools to investigate DNA damages in such complex systems as the nucleosome. In this mini-review, we summarize the latest advances brought out by computational approaches in the field, opening new exciting perspectives for the study of DNA damage and repair in the nucleosome context.
细胞 DNA 经常暴露于可诱发病变的内源性或外源性因素。紫外线/电离辐照、氧化应激或自由基等可导致多种类型的病变。为了克服这些损伤的有害影响,即诱变性或细胞毒性,细胞拥有高度复杂的 DNA 修复机制,其中包括通过专用细胞途径针对特定类型病变的修复酶。此外,DNA 在细胞核中高度压实,第一层压实由约 147 个 DNA 碱基对组成,包裹着组蛋白核心,即所谓的核小体核心颗粒。在这种复杂的环境中,DNA 结构受到高度约束,需要涉及重塑过程的微调机制,以使 DNA 暴露于修复酶,并促进损伤的清除。然而,人们对这些核糖体特异性机制的了解仍然很少,计算方法直到最近才成为研究核糖体等复杂系统中 DNA 损伤的有力工具。在这篇微型综述中,我们总结了该领域计算方法的最新进展,为研究核糖体背景下的 DNA 损伤和修复开辟了令人兴奋的新视角。
{"title":"DNA damage and repair in the nucleosome: insights from computational methods.","authors":"Natacha Gillet, Elise Dumont, Emmanuelle Bignon","doi":"10.1007/s12551-024-01183-9","DOIUrl":"10.1007/s12551-024-01183-9","url":null,"abstract":"<p><p>Cellular DNA is constantly exposed to endogenous or exogenous factors that can induce lesions. Several types of lesions have been described that can result from UV/ionizing irradiations, oxidative stress, or free radicals, among others. In order to overcome the deleterious effects of such damages, i.e., mutagenicity or cytotoxicity, cells possess a highly complex DNA repair machinery, involving repair enzymes targeting specific types of lesions through dedicated cellular pathways. In addition, DNA is highly compacted in the nucleus, the first level of compaction consisting of ~ 147 DNA base pairs wrapped around a core of histones, the so-called nucleosome core particle. In this complex environment, the DNA structure is highly constrained, and fine-tuned mechanisms involving remodeling processes are required to expose the DNA to repair enzymes and to facilitate the damage removal. However, these nucleosome-specific mechanisms remain poorly understood, and computational methods emerged only recently as powerful tools to investigate DNA damages in such complex systems as the nucleosome. In this mini-review, we summarize the latest advances brought out by computational approaches in the field, opening new exciting perspectives for the study of DNA damage and repair in the nucleosome context.</p>","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11297232/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}