In the present study, an attempt is made to reveal the functional groups and metabolites of gametophyte and sporophyte of two pteridophytes Anemia schimperiana C. Presl subsp. wightiana (Gardner) Fraser-Jenk. and Cyathea gigantea (Wall ex Hook.) Holtt using UV-Vis and FTIR analysis. For UV-Vis analysis, the ethanolic extracts of A. schimperiana subsp. wightiana and C. gigantea were centrifuged at 3000 rpm for 5 min and filtered using Whatman No. 1 filter paper. The ethanolic extracts were scanned and recorded using Shimadzu UV-Vis spectrophotometer at the absorbance of 200 to 1100 nm. For FTIR spectrometry about 1.0 mg of gametophyte and sporophyte ethanolic extracts of A. schimperiana subsp. wightiana and C. gigantea were separately made into very thin discs with potassium bromide and the pellets are measured in an automatic recording IR Spectrophotometer in the range of 4004000 cm1. Based on the observed peak values, the existence of alkaloids, phenolics, tannin and carotenoid existence were confirmed. Terpenoids, flavonoid and chlorophyll existence were also validated in different peak values of the ethanolic extracts of gametophytes and sporophytes of A. schimperiana subsp. wightiana and C. gigantea. The FTIR analysis results showed various functional groups viz., aromatic compounds, alkene, fluoro compounds, anhydride, aliphatic ether, alcohol, secondary amine presence in the gametophytes and sporophytes of A. schimperiana subsp. wightiana and C. gigantea. The gametophyte and sporophyte stages expressed the existence of different functional groups and displayed the variations and similarities between the studied stages. The spectroscopic results confirmed the secondary metabolites existence in the gametophytes and sporophytes of A. schimperiana subsp. wightiana and C. gigantea ethanolic extracts. Further chromatographic and separation studies may bring out stage-specific bioactive principles from the gametophytes and sporophytes of studied ferns.
本研究尝试使用紫外可见光分析和傅立叶变换红外光谱分析来揭示两种翼手目植物 Anemia schimperiana C. Presl subsp.在紫外可见光分析中，A. schimperiana subsp. wightiana 和 C. gigantea 的乙醇提取物在 3000 rpm 转速下离心 5 分钟，然后用 Whatman 1 号滤纸过滤。使用岛津紫外可见分光光度计扫描并记录乙醇提取物在 200 至 1100 纳米波长下的吸光度。用溴化钾分别将 A. schimperiana subsp. wightiana 和 C. gigantea 的配子体和孢子体乙醇提取物 1.0 毫克制成极薄的圆片，用自动记录式红外分光光度计在 4004000 厘米1 范围内测量圆片的傅立叶红外光谱。根据观察到的峰值，确认了生物碱、酚类、单宁和类胡萝卜素的存在。在 A. schimperiana subsp. wightiana 和 C. gigantea 的配子体和孢子体乙醇提取物的不同峰值中，萜类化合物、黄酮类化合物和叶绿素的存在也得到了验证。傅立叶变换红外光谱分析结果显示，A. schimperiana subsp. wightiana 和 C. gigantea 的配子体和孢子体中存在各种官能团，即芳香族化合物、烯、氟化合物、酸酐、脂肪醚、醇、仲胺。配子体和孢子体阶段表现出不同的功能群，并显示出研究阶段之间的差异和相似性。光谱结果证实了 A. schimperiana subsp. wightiana 和 C. gigantea 的配子体和孢子体乙醇提取物中存在次生代谢物。进一步的色谱和分离研究可能会从所研究蕨类植物的配子体和孢子体中发现特定阶段的生物活性成分。
{"title":"UV-VIS AND FTIR SPECTROSCOPIC PROFILE OF GAMETOPHYTE AND SPOROPHYTE ETHANOLIC EXTRACT OF ANEMIA SCHIMPERIANA C. PRESL SUBSP. WIGHTIANA (GARDNER) FRASER-JENK. AND CYATHEA GIGANTEA (WALL. EX. HOOK.) HOLTT.","authors":"Silvia Juliet Iruthayamani, M. Johnson","doi":"10.59277/rjb.2023.4.04","DOIUrl":"https://doi.org/10.59277/rjb.2023.4.04","url":null,"abstract":"In the present study, an attempt is made to reveal the functional groups and metabolites of gametophyte and sporophyte of two pteridophytes Anemia schimperiana C. Presl subsp. wightiana (Gardner) Fraser-Jenk. and Cyathea gigantea (Wall ex Hook.) Holtt using UV-Vis and FTIR analysis. For UV-Vis analysis, the ethanolic extracts of A. schimperiana subsp. wightiana and C. gigantea were centrifuged at 3000 rpm for 5 min and filtered using Whatman No. 1 filter paper. The ethanolic extracts were scanned and recorded using Shimadzu UV-Vis spectrophotometer at the absorbance of 200 to 1100 nm. For FTIR spectrometry about 1.0 mg of gametophyte and sporophyte ethanolic extracts of A. schimperiana subsp. wightiana and C. gigantea were separately made into very thin discs with potassium bromide and the pellets are measured in an automatic recording IR Spectrophotometer in the range of 4004000 cm1. Based on the observed peak values, the existence of alkaloids, phenolics, tannin and carotenoid existence were confirmed. Terpenoids, flavonoid and chlorophyll existence were also validated in different peak values of the ethanolic extracts of gametophytes and sporophytes of A. schimperiana subsp. wightiana and C. gigantea. The FTIR analysis results showed various functional groups viz., aromatic compounds, alkene, fluoro compounds, anhydride, aliphatic ether, alcohol, secondary amine presence in the gametophytes and sporophytes of A. schimperiana subsp. wightiana and C. gigantea. The gametophyte and sporophyte stages expressed the existence of different functional groups and displayed the variations and similarities between the studied stages. The spectroscopic results confirmed the secondary metabolites existence in the gametophytes and sporophytes of A. schimperiana subsp. wightiana and C. gigantea ethanolic extracts. Further chromatographic and separation studies may bring out stage-specific bioactive principles from the gametophytes and sporophytes of studied ferns.","PeriodicalId":90967,"journal":{"name":"Romanian journal of biophysics","volume":"37 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139007918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This work aims to inspect tomato features and classify them based on color and morphological features into the three predefined regions using artificial neural networks (ANN). Different learning methods were analyzed for the task of inspecting tomatoes using image processing software in MATLAB. Tomatoes were collected from the eastern parts of Ethiopia. The neural classification was done by the shape and size feature alone. The ANN classifier on the selected color feature alone showed that from the total test examples of 180 images, 168 (93.3) were correctly classified and 12 (6.7 %) were misclassified. The ANN classifier on all features taken together showed that all the test images were correctly classified. This result is similar to the morphology (shape and size) features result, but if the number of data points is high, the result may vary significantly. The overall result revealed that shape and size features have more discriminating power than color features, and the discrimination power increases when individual features are trained together with shape and size features. This may be because the discriminating factor increases due to the increase in the number of included features. It was observed that the proposed method was successful as quantified by the cumulative error (CE) and percentage error (%E) of training, testing, and validation of color features: 6.35 %, 3.70 %, and 11.11 %, respectively, in evaluating the quality of tomatoes.
{"title":"INSPECTION OF TOMATOES USING IMAGE PROCESSING TECHNIQUES","authors":"B. Girma, B. Goshu, E. Mengistu, M. Bodke","doi":"10.59277/rjb.2023.4.01","DOIUrl":"https://doi.org/10.59277/rjb.2023.4.01","url":null,"abstract":"This work aims to inspect tomato features and classify them based on color and morphological features into the three predefined regions using artificial neural networks (ANN). Different learning methods were analyzed for the task of inspecting tomatoes using image processing software in MATLAB. Tomatoes were collected from the eastern parts of Ethiopia. The neural classification was done by the shape and size feature alone. The ANN classifier on the selected color feature alone showed that from the total test examples of 180 images, 168 (93.3) were correctly classified and 12 (6.7 %) were misclassified. The ANN classifier on all features taken together showed that all the test images were correctly classified. This result is similar to the morphology (shape and size) features result, but if the number of data points is high, the result may vary significantly. The overall result revealed that shape and size features have more discriminating power than color features, and the discrimination power increases when individual features are trained together with shape and size features. This may be because the discriminating factor increases due to the increase in the number of included features. It was observed that the proposed method was successful as quantified by the cumulative error (CE) and percentage error (%E) of training, testing, and validation of color features: 6.35 %, 3.70 %, and 11.11 %, respectively, in evaluating the quality of tomatoes.","PeriodicalId":90967,"journal":{"name":"Romanian journal of biophysics","volume":"15 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139007440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
With the recent development in the field of electronics, it has made possible to continuous monitor health of patients and elderly and even of normal person. Since many years we are using blood-based diagnosis for diagnosis of various health conditions and diseases. However, recent research done by many researchers shows that there are other potential biomarkers such as, sweat which can provide diagnosis of various health conditions and diseases. Sodium and chloride concentration in sweat provides status of hydration status in human body. Here we have designed a health monitoring device based on sweat analysis using different types of electrochemical sensors. Ion-selective electrodes for Na+ and Cl- are used which gives details about a person’s hydration status and a glucose sensor is also used to measure the sweat glucose levels. The sensors are calibrated for artificial sweat solutions with different concentrations of Na+, Cl- and glucose. On body testing is done to confirm functionality of designed system for sweat analysis. The system is compact so can be used as a wearable device that is capable of continuously monitoring and wirelessly transmitting sensor data to the personal computer or smartphone.
{"title":"Wearable Health Monitoring System Based on Human Sweat Analysis using Electrochemical Sensors","authors":"Vandana Pagar, P.K. Bhadane, A.D. Shaligram","doi":"10.59277/rjb.2023.4.02","DOIUrl":"https://doi.org/10.59277/rjb.2023.4.02","url":null,"abstract":"With the recent development in the field of electronics, it has made possible to continuous monitor health of patients and elderly and even of normal person. Since many years we are using blood-based diagnosis for diagnosis of various health conditions and diseases. However, recent research done by many researchers shows that there are other potential biomarkers such as, sweat which can provide diagnosis of various health conditions and diseases. Sodium and chloride concentration in sweat provides status of hydration status in human body. Here we have designed a health monitoring device based on sweat analysis using different types of electrochemical sensors. Ion-selective electrodes for Na+ and Cl- are used which gives details about a person’s hydration status and a glucose sensor is also used to measure the sweat glucose levels. The sensors are calibrated for artificial sweat solutions with different concentrations of Na+, Cl- and glucose. On body testing is done to confirm functionality of designed system for sweat analysis. The system is compact so can be used as a wearable device that is capable of continuously monitoring and wirelessly transmitting sensor data to the personal computer or smartphone.","PeriodicalId":90967,"journal":{"name":"Romanian journal of biophysics","volume":"54 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139009700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Venkatesh, K. Yashaswini, L. S. Reddy Yadav, G. Nagaraju, A. Jayasheelan
Photinus scintillans is one of the species of fireflies, emits yellowish-green light with a peak wavelength of 575 nm. LaAlO3:Dy3+:Bi3+ nanomaterials prepared through sono-chemical process can serve as material for photo luminescence. The wavelength of light emitted by Photinus scintillans can also be obtained by optically exciting the synthesized sample with 353 nm wavelength. Dipole transition 4F9/2→6H13/2 gives photon of wavelength 576 nm, which is exactly same as that emitted by Photinus scintillans. The color chromaticity shows the yellowish green emission region of nanoparticles. This material can be used for the preparation of LEDs to emit a wavelength of 576 nm. When LEDs are prepared from this material, they can mimic the bioluminescence of fireflies. Thus, prepared nanoparticles can mimic the bioluminescence of Photinus scintillans fireflies. Both Dy doped and Bi co-doped samples at higher concentrations showed zone of clearance for Gram positive bacteria. Bi co-doped at higher concentrations had substantial inhibition across gram positive and negative bacterial colonies.
{"title":"SYNTHESIS OF LaAlO3:Dy3+:Bi3+ NANOMATERIALS TO OPTICALLY MIMIC THE BIOLUMINESCENCE OF PHOTINUS SCINTILLANS (FIREFLY) AND ANTIBACTERIAL APPLICATIONS","authors":"R. Venkatesh, K. Yashaswini, L. S. Reddy Yadav, G. Nagaraju, A. Jayasheelan","doi":"10.59277/rjb.2023.4.03","DOIUrl":"https://doi.org/10.59277/rjb.2023.4.03","url":null,"abstract":"Photinus scintillans is one of the species of fireflies, emits yellowish-green light with a peak wavelength of 575 nm. LaAlO3:Dy3+:Bi3+ nanomaterials prepared through sono-chemical process can serve as material for photo luminescence. The wavelength of light emitted by Photinus scintillans can also be obtained by optically exciting the synthesized sample with 353 nm wavelength. Dipole transition 4F9/2→6H13/2 gives photon of wavelength 576 nm, which is exactly same as that emitted by Photinus scintillans. The color chromaticity shows the yellowish green emission region of nanoparticles. This material can be used for the preparation of LEDs to emit a wavelength of 576 nm. When LEDs are prepared from this material, they can mimic the bioluminescence of fireflies. Thus, prepared nanoparticles can mimic the bioluminescence of Photinus scintillans fireflies. Both Dy doped and Bi co-doped samples at higher concentrations showed zone of clearance for Gram positive bacteria. Bi co-doped at higher concentrations had substantial inhibition across gram positive and negative bacterial colonies.","PeriodicalId":90967,"journal":{"name":"Romanian journal of biophysics","volume":"22 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139009665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J.H.M AL-SAEDI, MARIA MERNEA, GIORGIANA DIANA CARMEN ANGHELESCU, CRISTINA DOINA NIȚU, G. STOIAN, D.F. MIHĂILESCU
Protein glycation consists of the non-enzymatic attachment of monosaccharides to proteins. This leads to the formation of advanced glycation end products (AGEs) that are held responsible for diabetes complications. There are some drugs that inhibit AGEs, but their usage is limited by side effects. Plant-based therapeutic strategies could be useful in overcoming this limitation. Silybum marianum (Milk thistle), a plant used to treat liver problems, was also proved useful in the treatment of type 2 diabetes. Here we investigated the ability of S. marianum extract to inhibit the in vitro glycation of bovine serum albumin (BSA) by three physiologically relevant monosaccharides, namely glucose, fructose and galactose. BSA was glycated in the absence and in the presence of 0.1 %, 1 % and 5 % S. marianum seeds hydroalcoholic extract. Measurements on fructosamine, AGEs and amyloid cross-β structures formation showed that the plant extract inhibited these processes in the case of the three monosaccharides, especially in the case of glucose and galactose. The inhibition was dose- dependent and time-dependent. Our results demonstrate the ability of the plant extract to inhibit the in vitro glycation of BSA.
{"title":"THE INHIBITORY EFFECT OF SILYBUM MARIANUM (MILK THISTLE) SEEDS EXTRACT ON SERUM ALBUMIN GLYCATION BY GLUCOSE, FRUCTOSE AND GALACTOSE","authors":"J.H.M AL-SAEDI, MARIA MERNEA, GIORGIANA DIANA CARMEN ANGHELESCU, CRISTINA DOINA NIȚU, G. STOIAN, D.F. MIHĂILESCU","doi":"10.59277/rjb.2023.2.01","DOIUrl":"https://doi.org/10.59277/rjb.2023.2.01","url":null,"abstract":"Protein glycation consists of the non-enzymatic attachment of monosaccharides to proteins. This leads to the formation of advanced glycation end products (AGEs) that are held responsible for diabetes complications. There are some drugs that inhibit AGEs, but their usage is limited by side effects. Plant-based therapeutic strategies could be useful in overcoming this limitation. Silybum marianum (Milk thistle), a plant used to treat liver problems, was also proved useful in the treatment of type 2 diabetes. Here we investigated the ability of S. marianum extract to inhibit the in vitro glycation of bovine serum albumin (BSA) by three physiologically relevant monosaccharides, namely glucose, fructose and galactose. BSA was glycated in the absence and in the presence of 0.1 %, 1 % and 5 % S. marianum seeds hydroalcoholic extract. Measurements on fructosamine, AGEs and amyloid cross-β structures formation showed that the plant extract inhibited these processes in the case of the three monosaccharides, especially in the case of glucose and galactose. The inhibition was dose- dependent and time-dependent. Our results demonstrate the ability of the plant extract to inhibit the in vitro glycation of BSA.","PeriodicalId":90967,"journal":{"name":"Romanian journal of biophysics","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135786215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A.K. PANDEY, V.K. SINGH, A.K. SHARMA, S.N. TIWARI, G. MISHRA, R.K.S. YADAV, V.N. MISHRA
The piperidine put deep attention to research due to their pharmacological activity and hence drug designing. The derivatives of piperidine have important activities against cancers and tumor disease. Cancer is the most difficult disease in the world (WHO) which is basically considered to be the uncontrolled growth of abnormal cells. In present communication structural and vibrational features of piperidine derivatives 3-(hydroxymethyl)-3-methyl-2,6-diphenylpiperidin-4-one has been computed based on theoretical quantum chemical approach. The primary aspects of piperidine derivatives 3-(hydroxymethyl)-3-methyl-2,6-diphenylpiperidin-4-one structure has been calculated by using optimized geometry, spectroscopic behavior, chemical reactivity and molecular docking analysis. The info about coupled of modes were founded in vibrational features of the molecule. The stability of the chemical reactivity of 3-(hydroxymethyl)-3-methyl-2,6-diphenylpiperidin-4-one was predicted by using HOMO-LUMO energy gap. The molecular electrostatic surface potential (MESP) plot utilizes overall picture of accretion of charges on separate atoms in molecule which very useful predict the nucleophilic and electrophilic charge center. The pharmacological importance of piperidine derivatives 3-(hydroxymethyl)-3-methyl-2,6-diphenylpiperidin-4-one is established by calculated biological activity. The inhibition properties and chemical reactivity of piperidine derivatives 3-(hydroxymethyl)- 3-methyl-2,6-diphenylpiperidin-4-one are well-known by chemical reactivity descriptors by using HOMO-LUMO energies. The inhibition potentials of piperidine derivatives 3-(hydroxymethyl)- 3-methyl-2,6-diphenylpiperidin-4-one are established by calculated lipophilicity, aqueous solubility, and binding affinity. The docking of molecule has been also performed with suitable target.
{"title":"SPECTROSCOPIC, NLO, NBO, BIOCHEMICAL INVESTIGATION OF 3-(HYDROXYMETHYL)-3-METHYL-2,6-DIPHENYLPIPERIDIN-4-ONE","authors":"A.K. PANDEY, V.K. SINGH, A.K. SHARMA, S.N. TIWARI, G. MISHRA, R.K.S. YADAV, V.N. MISHRA","doi":"10.59277/rjb.2023.2.03","DOIUrl":"https://doi.org/10.59277/rjb.2023.2.03","url":null,"abstract":"The piperidine put deep attention to research due to their pharmacological activity and hence drug designing. The derivatives of piperidine have important activities against cancers and tumor disease. Cancer is the most difficult disease in the world (WHO) which is basically considered to be the uncontrolled growth of abnormal cells. In present communication structural and vibrational features of piperidine derivatives 3-(hydroxymethyl)-3-methyl-2,6-diphenylpiperidin-4-one has been computed based on theoretical quantum chemical approach. The primary aspects of piperidine derivatives 3-(hydroxymethyl)-3-methyl-2,6-diphenylpiperidin-4-one structure has been calculated by using optimized geometry, spectroscopic behavior, chemical reactivity and molecular docking analysis. The info about coupled of modes were founded in vibrational features of the molecule. The stability of the chemical reactivity of 3-(hydroxymethyl)-3-methyl-2,6-diphenylpiperidin-4-one was predicted by using HOMO-LUMO energy gap. The molecular electrostatic surface potential (MESP) plot utilizes overall picture of accretion of charges on separate atoms in molecule which very useful predict the nucleophilic and electrophilic charge center. The pharmacological importance of piperidine derivatives 3-(hydroxymethyl)-3-methyl-2,6-diphenylpiperidin-4-one is established by calculated biological activity. The inhibition properties and chemical reactivity of piperidine derivatives 3-(hydroxymethyl)- 3-methyl-2,6-diphenylpiperidin-4-one are well-known by chemical reactivity descriptors by using HOMO-LUMO energies. The inhibition potentials of piperidine derivatives 3-(hydroxymethyl)- 3-methyl-2,6-diphenylpiperidin-4-one are established by calculated lipophilicity, aqueous solubility, and binding affinity. The docking of molecule has been also performed with suitable target.","PeriodicalId":90967,"journal":{"name":"Romanian journal of biophysics","volume":"68 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135786213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
"The biomedical potential of destabilizing liposomes through photoinduction relies on the use of near-infrared light, which offers inherent therapeutic advantages. Researchers have explored the effects of infrared laser light on dipalmitoyl phosphatidylcholine (DPPC) multilamellar vesicles, specifically investigating the interaction between the laser and zwitterionic dipalmitoyl phosphatidylcholine multilamellar vesicles using Fourier transform infrared spectroscopy and differential scanning calorimetry measurements. The results revealed that laser irradiation increased the number of gauche conformers and led to conformational changes within the acyl chains of the phospholipids. The transition temperature of lyophilized vesicles was also shifted to a lower temperature after laser irradiation, indicating that the laser had a dipalmitoyl phosphatidylcholine multilamellar significant effect on the acyl chains in the dipalmitoyl phosphatidylcholine bilayers and decreased the transition cooperativity of lipid acyl chains. These findings could aid in the development of more effective liposomal drug delivery systems by enhancing our understanding of the interaction between laser and lipid bilayers."
{"title":"LASER INDUCED CONFORMATIONAL CHANGES OF LIPIDS WITHIN MULTILAMELLAR VESICLES","authors":"M. MADY, MANAL HUSSIEN","doi":"10.59277/rjb.2023.2.02","DOIUrl":"https://doi.org/10.59277/rjb.2023.2.02","url":null,"abstract":"\"The biomedical potential of destabilizing liposomes through photoinduction relies on the use of near-infrared light, which offers inherent therapeutic advantages. Researchers have explored the effects of infrared laser light on dipalmitoyl phosphatidylcholine (DPPC) multilamellar vesicles, specifically investigating the interaction between the laser and zwitterionic dipalmitoyl phosphatidylcholine multilamellar vesicles using Fourier transform infrared spectroscopy and differential scanning calorimetry measurements. The results revealed that laser irradiation increased the number of gauche conformers and led to conformational changes within the acyl chains of the phospholipids. The transition temperature of lyophilized vesicles was also shifted to a lower temperature after laser irradiation, indicating that the laser had a dipalmitoyl phosphatidylcholine multilamellar significant effect on the acyl chains in the dipalmitoyl phosphatidylcholine bilayers and decreased the transition cooperativity of lipid acyl chains. These findings could aid in the development of more effective liposomal drug delivery systems by enhancing our understanding of the interaction between laser and lipid bilayers.\"","PeriodicalId":90967,"journal":{"name":"Romanian journal of biophysics","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135786214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the present study, FT-IR analysis was used to study the functional groups present in the three different extracts of in vitro cultured gametophytes and sporophytes of Phlebodium aureum (L.) J. Smith. The powdered sample of each sample was loaded in FT-IR spectrophotometer (Shimadzu 8400S) in the range of 400 to 4000 cm–1 . The transmission percentage was verified against the wave number. The peak values of FT-IR were recorded, and the functional groups were predicted using Aldrich and Sigma IR chart table. The analysis revealed the presence of different types of compounds such as alkene, sulfoxide, esters, aromatic compounds, isothiocyanate etc. The observed results showed the similarities and variation between the gametophytes and sporophytes of Phlebodium aureum. The outcome of the study showed the unique profiles for gametophytes and sporophytes, and these may be employed to identify the gametophytes and sporophytes of Phlebodium aureum.
{"title":"FT-IR SPECTROSCOPIC STUDIES ON THE GAMETOPHYTES AND SPOROPHYTES OF PHLEBODIUM AUREUM(L.) J. SMITH ","authors":"GEORGE VIDYARANI, M. JOHNSON, M. GLORY","doi":"10.59277/rjb.2023.1.02","DOIUrl":"https://doi.org/10.59277/rjb.2023.1.02","url":null,"abstract":"In the present study, FT-IR analysis was used to study the functional groups present in the three different extracts of in vitro cultured gametophytes and sporophytes of Phlebodium aureum (L.) J. Smith. The powdered sample of each sample was loaded in FT-IR spectrophotometer (Shimadzu 8400S) in the range of 400 to 4000 cm–1 . The transmission percentage was verified against the wave number. The peak values of FT-IR were recorded, and the functional groups were predicted using Aldrich and Sigma IR chart table. The analysis revealed the presence of different types of compounds such as alkene, sulfoxide, esters, aromatic compounds, isothiocyanate etc. The observed results showed the similarities and variation between the gametophytes and sporophytes of Phlebodium aureum. The outcome of the study showed the unique profiles for gametophytes and sporophytes, and these may be employed to identify the gametophytes and sporophytes of Phlebodium aureum.","PeriodicalId":90967,"journal":{"name":"Romanian journal of biophysics","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136296304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alzheimer’s disease (AD) is a degrading and irreversible neurodegenerative disorder associated with loss of brain functions. It is manifested clinically into psychological symptoms that collectively form cognitive dysfunction and the loss of thinking skills through the progressive degeneration of central nervous system neurons. Over the last decade, γ-secretase emerged as a promising target for the treatment of Alzheimer’s disease. The γ-secretase modulator compounds would be good candidates for AD therapeutics. In previous years, a series fused 5,6-bicyclic heterocycles were investigated as γ-secretase modulators or anti Alzheimer’s agents. In the present study, a quantitative structure activity relationship (QSAR) study of twenty-eight derivatives of fused 5,6-bicyclic heterocycles has been made with the help of topological parameters. The descriptors that have been used are solvent accessible surface area, valence connectivity indices of order 0, 1 and 2 and shape indices of order 1, 2 and 3. The best QSAR model for this set of derivatives has been obtained by using solvent accessible surface area as first descriptor, valence connectivity index (order-0) as second descriptor, valence connectivity index (order-1) as third descriptor and shape index (order-3) as fourth descriptor. The correlation coefficient (r 2 ) and cross validation coefficient (rCV2 ) for this model are 0.773037 and 0.688568, respectively.
{"title":"2D QSAR STUDY OF FUSED 5,6-BICYCLIC HETEROCYCLES AS ANTI ALZHEIMER’S AGENTS USING TOPOLOGICAL PARAMETERS","authors":"S.K MISRA, PRITI SINGH, R.K. SINGH, G.K SRIVASTAV","doi":"10.59277/rjb.2023.1.01","DOIUrl":"https://doi.org/10.59277/rjb.2023.1.01","url":null,"abstract":"Alzheimer’s disease (AD) is a degrading and irreversible neurodegenerative disorder associated with loss of brain functions. It is manifested clinically into psychological symptoms that collectively form cognitive dysfunction and the loss of thinking skills through the progressive degeneration of central nervous system neurons. Over the last decade, γ-secretase emerged as a promising target for the treatment of Alzheimer’s disease. The γ-secretase modulator compounds would be good candidates for AD therapeutics. In previous years, a series fused 5,6-bicyclic heterocycles were investigated as γ-secretase modulators or anti Alzheimer’s agents. In the present study, a quantitative structure activity relationship (QSAR) study of twenty-eight derivatives of fused 5,6-bicyclic heterocycles has been made with the help of topological parameters. The descriptors that have been used are solvent accessible surface area, valence connectivity indices of order 0, 1 and 2 and shape indices of order 1, 2 and 3. The best QSAR model for this set of derivatives has been obtained by using solvent accessible surface area as first descriptor, valence connectivity index (order-0) as second descriptor, valence connectivity index (order-1) as third descriptor and shape index (order-3) as fourth descriptor. The correlation coefficient (r 2 ) and cross validation coefficient (rCV2 ) for this model are 0.773037 and 0.688568, respectively.","PeriodicalId":90967,"journal":{"name":"Romanian journal of biophysics","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136296303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The emanation of gases from the walls, floors, and ceilings is the main source of radon (Rn) and thoron (Tn) in enclosed rooms. The bulk of terrestrial construction materials contain gas in their pores in concentrations 1,000−10,000 times higher than those in the atmosphere. This high concentration results in a considerable radon/thoron concentration difference between the construction materials and ambient air. In this study, indoor radon, thoron, and their progeny concentrations, in Bangalore Metropolitan households, were measured using a significant number of dosimeters.
{"title":"STUDY OF THE CONNECTION BETWEEN ROOM SIZE AND RADON/THORON DOSE RATES IN BANGALORE METROPOLITAN AREA ","authors":"L.A. Sathish","doi":"10.59277/rjb.2023.1.03","DOIUrl":"https://doi.org/10.59277/rjb.2023.1.03","url":null,"abstract":"The emanation of gases from the walls, floors, and ceilings is the main source of radon (Rn) and thoron (Tn) in enclosed rooms. The bulk of terrestrial construction materials contain gas in their pores in concentrations 1,000−10,000 times higher than those in the atmosphere. This high concentration results in a considerable radon/thoron concentration difference between the construction materials and ambient air. In this study, indoor radon, thoron, and their progeny concentrations, in Bangalore Metropolitan households, were measured using a significant number of dosimeters.","PeriodicalId":90967,"journal":{"name":"Romanian journal of biophysics","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136296305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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