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Effect of time and wattage power levels of microwave treatment on the microbial quality and safety of bovine raw milk 微波处理时间和功率水平对牛乳微生物质量和安全性的影响
Pub Date : 2022-09-18 DOI: 10.18502/jfsh.v8i2.10674
Fatemeh Shahvandari, Nader Akbari, G. Jahed Khaniki, N. Shariatifar, Seyedeh Maryam Mirsharifi
Raw milk is sterile when secreted by healthy cows, however it is contaminated with different microorganisms and Streptococci commonly present in the milk ducts as well as teats. The bacterial count in raw milk ranges from a few hundred to several thousand per mL of milk, however it is harmful to human health under unpasteurized condition. Milk sanitization methods rely on the principle of preventing the growth and development of microbes and thus maintaining their nutritional quality. This study was carried out to investigate the effect of microwave radiation on the microbial quality and safety of bovine raw milk. To do so, the effect of microwaves at a frequency of 35 KH and powers of 180, 300 and 850 W for 0, 30, 60 and 90 s on the destruction of pathogens and reduction of microbial load of milk (total bacterial count, coliforms, Escherichia coli, Staphylococcus aureus, molds and yeasts) was investigated. The results showed that microwave treatment had a significant effect on reducing the number of microorganisms in milk samples (p<0.0) with the power of 850 W being the most effective power for reducing the number of S. aureus, coliforms, molds, yeasts as well as total bacterial count. Faster heating with higher energy efficiency is the main advantage of the microwave process for foods. In fact, the microwave method can be a potential and effective method for decreasing the microbial load of raw milk.
健康奶牛分泌的生乳是无菌的,但它被不同的微生物和链球菌污染,链球菌通常存在于乳管和乳头中。生牛奶中的细菌数量从每毫升牛奶几百到几千不等,但在未经高温消毒的条件下对人体健康有害。牛奶消毒方法的原理是防止微生物的生长和发育,从而保持其营养质量。本研究旨在研究微波辐射对牛乳微生物质量和安全性的影响。为此,研究了频率为35KH、功率为180300W和850W、持续0、30、60和90s的微波对牛奶中病原体的破坏和微生物负荷(细菌总数、大肠菌群、大肠杆菌、金黄色葡萄球菌、霉菌和酵母)的减少的影响。结果表明,微波处理对降低牛奶样品中的微生物数量有显著作用(p<0.0),其中850 W的功率是降低金黄色葡萄球菌、大肠菌群、霉菌、酵母数量和细菌总数的最有效功率。更快的加热和更高的能源效率是微波处理食品的主要优势。事实上,微波法是一种潜在而有效的降低原料奶微生物负荷的方法。
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引用次数: 0
Nutritious food and health risks: a review on the edible land snails of Africa 营养食品和健康风险:非洲可食陆地蜗牛研究综述
Pub Date : 2022-09-18 DOI: 10.18502/jfsh.v8i2.10669
Mary Nkongho Tanyitiku
Snail meat is a source of food and livelihood for many inhabitants especially in sub-Saharan Africa. They are consumed in households or traded at local and international markets. However, African land snails are rarely farmed but picked from environments that include decaying vegetation, soil debris, and untreated human and household wastes. These ‘snail natural habitats’ may contain microbial pathogens that could easily be ingested by snails and transmitted to snail meat handlers and consumers. The objective of this study was to investigate published information that establishes the local consumption practices of African land snails as a credible source of foodborne infections. Acknowledging the nutritional benefits of consuming snails, this research revealed local practices in snail gathering, handling, preparation and preservation could significantly contribute to foodrelated disease burdens to Africans and several African snail meat-exporting countries. With this, national and international food safety regulations are required for African snail meat consumption.
蜗牛肉是许多居民的食物和生计来源,尤其是在撒哈拉以南非洲。它们在家庭中消费或在当地和国际市场进行交易。然而,非洲陆地蜗牛很少被养殖,而是从腐烂的植被、土壤碎片以及未经处理的人类和家庭垃圾等环境中采摘。这些“蜗牛自然栖息地”可能含有微生物病原体,这些病原体很容易被蜗牛摄入并传播给蜗牛肉处理者和消费者。这项研究的目的是调查已发表的信息,这些信息表明非洲陆地蜗牛的当地消费行为是食源性感染的可靠来源。这项研究承认食用蜗牛的营养益处,表明当地在蜗牛采集、处理、制备和保存方面的做法可能会大大加重非洲人和几个非洲蜗牛肉出口国的食品相关疾病负担。因此,非洲蜗牛肉消费需要国家和国际食品安全法规。
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引用次数: 0
Phytochemical content, antioxidant properties and antibacterial activities of date (Phoenix dactylifera L.) seed extracts 枣(Phoenix dactylifera L.)种子提取物的植物化学成分、抗氧化性能和抗菌活性
Pub Date : 2022-09-18 DOI: 10.18502/jfsh.v8i2.10670
O. Olaniyi, Ifedolapo Abigeal Samuel, F. O. Igbe
This study evaluated the antioxidant and antibacterial properties of date (Phoenix dactylifera L.) seed extracts against some bacterial pathogens of food-borne diseases. The phytochemical constituents and antioxidant properties of the date seed were determined using a standard chemical method. In vitro antibacterial activities of the crude extracts of date seeds against the pathogens were determined using the agar well diffusion method. The phytochemical screenings of the extracts revealed the presence of oxalate, phytate, flavonoid, saponin, tannin and cardiac glycoside. The screening of the date seed extracts for antioxidant compounds revealed varied concentrations of total antioxidant capacity, ferric reducing antioxidant property and flavonoid. Methanol and aqueous date seed extracts exhibited appreciable antibacterial activity against E. coli, K. pneumoniae, S. typhimurium, B. subtilis and S. flexneri whereas, n-hexane extract had a mild effect on all test organisms. At 200 and 100 mg/mL of the crude extracts, all the test isolates were inhibited. Varied Minimum inhibitory and Minimum Bactericidal Concentrations of different date seeds extracts showed potential bacteriostatic and bactericidal action against the test pathogens. It can be deduced that the important bioactive compounds in date seeds may be responsible for the observed antibacterial activity against the causative agents of food-borne illnesses.
研究了枣(Phoenix dactylifera L.)种子提取物对食源性病原菌的抗氧化和抑菌作用。采用标准化学方法测定了枣子的植物化学成分和抗氧化性能。采用琼脂孔扩散法测定了枣种子粗提物对病原菌的体外抑菌活性。植物化学筛选结果显示,提取物中含有草酸盐、植酸盐、类黄酮、皂苷、单宁和心糖苷。对枣子提取物的抗氧化性进行了筛选,发现其总抗氧化能力、铁还原抗氧化能力和类黄酮含量各不相同。甲醇和水红枣籽提取物对大肠杆菌、肺炎克雷伯菌、鼠伤寒沙门氏菌、枯草芽孢杆菌和flexneri沙门氏菌具有明显的抑菌活性,而正己烷提取物对所有试验生物均有轻微的抑菌作用。在200和100 mg/mL粗提物浓度下,所有分离株均被抑制。不同枣籽提取物的最低抑菌浓度和最低杀菌浓度不同,对试验病原菌具有潜在的抑菌和杀菌作用。由此可以推断,所观察到的对食源性疾病病原体的抗菌活性可能与枣籽中重要的生物活性化合物有关。
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引用次数: 0
Smallholder farmer's awareness and determination of aflatoxins in sesame seeds of southern district of Tanzania 坦桑尼亚南部地区小农对芝麻中黄曲霉毒素的认识及测定
Pub Date : 2022-09-18 DOI: 10.18502/jfsh.v8i2.10671
Catherine Gidabedi, N. Kassim, A. Matemu
Consumption of aflatoxins contaminated foods has led to detrimental health effects worldwide, with even more severe cases in African countries including Tanzania. A cross-sectional study was conducted to assess awareness and aflatoxins contamination of sesame seeds in Lindi and Mtwara regions. Subsequently, a total of 70 sesame seed samples were randomly purchased from local markets for aflatoxin determination using High Performance Liquid Chromatography (HPLC). Qualitative data were analyzed using SPSS 20 for descriptive and correlation analysis. Results show that 82.4% of the respondents were not aware of aflatoxin contamination of agricultural produce. Awareness was negatively correlated to the levels of education (p=-0.309) and positively correlated with gender whereby men were more aware than women (p=0.03). On the other hand, 37 out of 70 sesame seed samples were contaminated with total aflatoxins at a range of 0.009 ng/g to 5.557 ng/g. Although none of these samples exceeded the Tanzania maximum limits of 10 ng/g for total aflatoxins, 2 samples exceeded the maximum limit of 4 ng/g set by the European Union. Furthermore, Aflatoxin B1 was detected in 13 samples moreover the concentration was below the Tanzania and EU maximum limits of 5 ng/g and 2 ng/g respectively. Though the contamination was below the national maximum limits and limited to one agro-ecological zone and season, these findings provide useful insights on aflatoxins contamination of sesame seeds from the two main growing regions in Tanzania.
食用受黄曲霉毒素污染的食品对全世界的健康造成了有害影响,包括坦桑尼亚在内的非洲国家出现了更严重的病例。进行了一项横断面研究,以评估林迪和姆特瓦拉地区对芝麻的认识和黄曲霉毒素污染情况。随后,从当地市场随机购买了70份芝麻样品,用高效液相色谱法(HPLC)测定黄曲霉毒素。定性数据采用SPSS 20进行描述性和相关性分析。结果显示,82.4%的受访者不知道农产品中的黄曲霉毒素污染。意识与教育水平呈负相关(p=0.0309),与性别呈正相关,男性比女性更有意识(p=0.03)。另一方面,70个芝麻样本中有37个样本被总黄曲霉毒素污染,污染范围为0.009纳克/克至5.557纳克/克。尽管这些样本中没有一个超过坦桑尼亚黄曲霉毒素总量10纳克/克的最高限值,但有2个样本超过了欧盟规定的4纳克/克最高限值。此外,在13个样品中检测到黄曲霉毒素B1,其浓度分别低于坦桑尼亚和欧盟的最大限值5纳克/克和2纳克/克。尽管污染低于国家最高限值,仅限于一个农业生态区和季节,但这些发现为坦桑尼亚两个主要种植区芝麻的黄曲霉毒素污染提供了有用的见解。
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引用次数: 0
The risk of Campylobacter jejuni and Campylobacter coli associated with the consumption of fresh camel milk in Qatar 卡塔尔新鲜骆驼奶消费与空肠弯曲杆菌和大肠弯曲杆菌的风险
Pub Date : 2022-07-03 DOI: 10.18502/jfsh.v8i1.9959
H. Mohammed, Kenlyn E. Peters, Irene Rojas Nunez, A. Salem, S. Doiphode, A. Sultan
Campylobacter spp. are among the ten top leading causes of foodborne diseases around the world and are capable of triggering severe gastroenteritis with potential for severe sequelae. Fresh camel milk is one of the most common hospitality gestures among certain cultures. We investigated the potential risk of illness from the consumption of camel milk contaminated with Campylobacter spp. in Qatar and identified critical intervention points that would contribute to mitigating its consequences. Quantitative risk assessment (QRA) methodology with a combination of deterministic and stochastic approaches was employed to address this objective. Data on the likelihood of either C. jejuni or C. coli in camel milk or in humans was obtained through repeat cross-sectional studies in these populations in Qatar. Estimates of the adverse health effects were computed using risk characterization which integrated data on the hazard, the probability of exposure, and dose-response models. Our analyses showed that the probability of illness for a healthy female from the consumption of camel milk contaminated with C. jejuni ranged from 5 x 10-3 to 24 x 10-2 depending on the amount of milk consumed. The risk of illness for males was higher (13 x 10-3 to 30 x 10-2). The estimates of illness were three times higher for immunocompromised females in comparison to healthy females. Immunocompromised males had five-times higher risk of illness in comparison to healthy men. The computed risk of illness from consumption of camel milk contaminated with either C. jejuni or C. coli could be significantly mitigated by increasing the efficacy of sterilizing or pasteurization before serving.
弯曲杆菌属是世界各地食源性疾病的十大主要病因之一,能够引发严重的胃肠炎,并可能造成严重的后遗症。新鲜的骆驼奶是某些文化中最常见的款待方式之一。我们调查了卡塔尔食用被弯曲杆菌污染的骆驼奶的潜在疾病风险,并确定了有助于减轻其后果的关键干预点。定量风险评估(QRA)方法与确定性和随机方法相结合,以解决这一目标。通过对卡塔尔这些人群的重复横断面研究,获得了骆驼奶或人体内存在空肠梭菌或大肠杆菌可能性的数据。利用综合了危害数据、暴露概率和剂量反应模型的风险特征来计算不良健康影响的估计值。我们的分析表明,健康雌性因食用被空肠梭菌污染的骆驼奶而患病的概率在5 × 10-3至24 × 10-2之间,具体取决于所食用的奶量。男性患病风险较高(13 × 10-3 ~ 30 × 10-2)。与健康女性相比,免疫功能低下的女性患病的估计是健康女性的三倍。免疫功能低下的男性患病风险是健康男性的5倍。食用被空肠梭菌或大肠杆菌污染的骆驼奶所计算的患病风险,可以通过提高食用前消毒或巴氏消毒的效果来显著降低。
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引用次数: 0
Status of aflatoxin B1 in rice and rice products from Jhapa district of Nepal 尼泊尔贾帕县大米和大米制品中黄曲霉毒素B1的状况
Pub Date : 2022-07-03 DOI: 10.18502/jfsh.v8i1.9961
Devraj Acharya, Lekhraj Dhakal, Santosh Thapa
Occurrence of aflatoxin in staple food products is a serious threat to public health. This study was aimed to determine the level of aflatoxin B1 (AFB1) contamination in rice and rice products produced in Jhapa, a major rice producing area of the country. A total of 108 samples including paddy, rice and rice products (4 varieties each) were collected and the amount of AFB1 in them was analyzed using Bio-Shield B1 5 ELISA test. The major varieties of paddy cultivatedwere Ranjit (26.61%), Sarana (22.22%), NR-2167 (13.89%) and Sukkha variety (5.56%). Fungal attack, color change and unwanted odor were major problems incurred during paddy storage while fungal attack and appearance of lumps were major problems during rice storage. About 76.92% respondents were unaware of good agricultural practices and 87% of them had no idea about aflatoxins. Through ELISA, it was found that paddy, rice and rice products had a mean AFB1 content of 1.43, 1.41 and 1.64 μg/kg respectively, and the contamination levels differed significantly among different varieties of the samples. Ranjit variety of paddy, rice as well as beaten rice had the highest level of contamination among paddy, rice and rice product samples respectively. All the samples had AFB1 concentration below the standards set by Nepal Government as well as World Health Organization. But 1 sample of Sukkha paddy, 3 samples of Ranjit paddy, 1 sample of Mansoori rice, 3 samples of Ranjit rice, 3 samples of Ranjit beaten rice and 3 samples of Mansoori puffed rice had AFB1 above the European Union standards.
主要食品中黄曲霉毒素的出现严重威胁着公众的健康。本研究旨在确定该国主要水稻产区Jhapa生产的大米和大米制品中黄曲霉毒素B1 (AFB1)的污染水平。采集稻谷、稻米及稻制品共108份(各4个品种)样品,采用Bio-Shield B1 5酶联免疫吸附试验(ELISA)检测AFB1的含量。水稻品种主要为兰吉特(26.61%)、沙拉纳(22.22%)、NR-2167(13.89%)和苏卡(5.56%)。真菌侵染、颜色变化和异味是水稻贮藏期间的主要问题,真菌侵染和结块是水稻贮藏期间的主要问题。76.92%的受访者不了解良好农业规范,87%的受访者不了解黄曲霉毒素。通过酶联免疫吸附试验发现,稻谷、大米和米制品中AFB1的平均含量分别为1.43、1.41和1.64 μg/kg,不同品种样品的污染程度差异显著。兰吉特品种的水稻、大米和打过的大米样品中污染程度最高。所有样本的AFB1浓度均低于尼泊尔政府和世界卫生组织规定的标准。但1个Sukkha水稻样本、3个Ranjit水稻样本、1个Mansoori水稻样本、3个Ranjit水稻样本、3个Ranjit打浆大米样本和3个Mansoori膨化大米样本的AFB1含量高于欧盟标准。
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引用次数: 0
Evaluating the antimicrobial effect of postbiotic extract from Lactobacillus casei on Escherichia coli in commercial sterilized milk 评价干酪乳杆菌生后提取物对商品灭菌乳中大肠杆菌的抑菌效果
Pub Date : 2022-07-03 DOI: 10.18502/jfsh.v8i1.9960
S. Hosseini, A. Homayouni‐Rad, H. Samadi kafil, Nazli Dorud
The antimicrobial effect postbiotic extant of Lactobacillus casei was evaluated against Escherichia coli by well propagation method and growth inhibition zone diameter was measured. The selected concentration of the postbiotic extract of Lactobacillus casei was added to milk containing E. coli, followed by evaluating the changes in colony number and pH during storage (1 and 5) days at 4°C then compared with control milk. The largest growth inhibition zone was formed at a concentration of 800 µl/ml against E. coli. Also, the counts of E. coli decreased on days 1 and 5 in the evaluated milk containing postbiotic (p≤ 0.05). The pH of milk containing postbiotic was 6.5-6.8 (p≤ 0.05). The results showed that the postbiotics of L. casei inhibited the growth of E. coli in milk containing.
采用良好繁殖法评价干酪乳杆菌对大肠杆菌的抗菌效果,并测定生长抑制区直径。将选定浓度的干酪乳杆菌生后提取物添加到含有大肠杆菌的牛奶中,然后评估在4°C下储存(1和5)天期间菌落数和pH的变化,然后与对照牛奶进行比较。在对大肠杆菌的浓度为800µl/ml时,形成了最大的生长抑制区。此外,在评估的含有益生元的牛奶中,大肠杆菌的计数在第1天和第5天下降(p≤0.05)。含有益生素的牛奶的pH值为6.5-6.8(p≤0.05.结果表明,干酪乳杆菌的益生元抑制了含益生元牛奶中大肠杆菌的生长。
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引用次数: 2
The content and dietary exposure of Malondialdehyde in industrial and traditional ice cream fats 工业和传统冰淇淋脂肪中丙二醛的含量及膳食暴露
Pub Date : 2022-07-03 DOI: 10.18502/jfsh.v8i1.9958
P. Sadighara, R. Shahbazi, Mohammad Zirak, Sara Mohamadi, L. Karami, Nooshin Zomorodian, A. Abedini
Lipid oxidation is an undesirable reaction that produces unwanted and harmful compounds, including malondialdehyde (MDA). Ice cream has a considerable amount of fat; therefore, it is sensitive to lipid oxidation. The primary purpose of this study was to determine the lipid oxidation level in traditional and pasteurized ice creams. The traditional ice cream and various brands of pasteurized ice cream samples were collected. The amount of MDA was measured by the TBARS method, and dietary exposure to MDA was calculated. MDA level in the traditional samples were lower than in pasteurized ice creams, however it was not significant (p>0.05). The level of lipid oxidation was different in the brands A, B, and C. The highest and lowest levels of MDA were found in brand A and brand C, respectively. The value of estimated dietary intake was calculated as 4.251 µg/Kg. This study showed that the amount of MDA could be very different in branded samples. Furthermore, the dietary intake of MDA is considerable. Therefore, it is necessary to develop a standard regarding the permissible level of MDA in ice cream.
脂质氧化是一种不良反应,会产生不需要的有害化合物,包括丙二醛(MDA)。冰淇淋含有相当多的脂肪;因此,对脂质氧化很敏感。本研究的主要目的是确定传统和巴氏消毒冰淇淋中的脂质氧化水平。采集了传统冰淇淋和各品牌巴氏灭菌冰淇淋样品。用TBARS法测定MDA的含量,并计算饮食中MDA的暴露量。传统冰淇淋中的丙二醛含量低于巴氏消毒冰淇淋,但差异不显著(p < 0.05)。脂质氧化水平在A、B、C三个品牌中存在差异,MDA含量在A和C品牌中分别最高和最低。估算膳食摄入量为4.251µg/Kg。这项研究表明,在品牌样品中,丙二醛的含量可能会有很大的不同。此外,膳食中丙二醛的摄入量是相当可观的。因此,有必要制定一个关于冰淇淋中丙二醛允许含量的标准。
{"title":"The content and dietary exposure of Malondialdehyde in industrial and traditional ice cream fats","authors":"P. Sadighara, R. Shahbazi, Mohammad Zirak, Sara Mohamadi, L. Karami, Nooshin Zomorodian, A. Abedini","doi":"10.18502/jfsh.v8i1.9958","DOIUrl":"https://doi.org/10.18502/jfsh.v8i1.9958","url":null,"abstract":"Lipid oxidation is an undesirable reaction that produces unwanted and harmful compounds, including malondialdehyde (MDA). Ice cream has a considerable amount of fat; therefore, it is sensitive to lipid oxidation. The primary purpose of this study was to determine the lipid oxidation level in traditional and pasteurized ice creams. The traditional ice cream and various brands of pasteurized ice cream samples were collected. The amount of MDA was measured by the TBARS method, and dietary exposure to MDA was calculated. MDA level in the traditional samples were lower than in pasteurized ice creams, however it was not significant (p>0.05). The level of lipid oxidation was different in the brands A, B, and C. The highest and lowest levels of MDA were found in brand A and brand C, respectively. The value of estimated dietary intake was calculated as 4.251 µg/Kg. This study showed that the amount of MDA could be very different in branded samples. Furthermore, the dietary intake of MDA is considerable. Therefore, it is necessary to develop a standard regarding the permissible level of MDA in ice cream.","PeriodicalId":91000,"journal":{"name":"Journal of food safety and hygiene","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45521356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
An overview of genetically modified foods: agreement, challenges and assessment of safety 转基因食品概述:协议、挑战和安全性评估
Pub Date : 2022-07-03 DOI: 10.18502/jfsh.v8i1.9956
Mahsa Alikord, Mohadeseh Pirhadi, S. Shokri, N. Shariatifar
The technology of genetically modified (GM) can overwhelm agricultural and nutritional difficulties in the food industry, food safety and security by increasing resistance to pests and herbicides, drought tolerance, rapid ripening and ultimately increasing yield and food quality. However, in the last few decades, significant dangers of GM foods to humans, animals, and the environment have been identified. Nevertheless, there is insufficient scientific evidence to prove the harmful effects of these foods on human and animal health. In this article, several advantages and disadvantages of this technology are reviewed. Therefore, it is necessary to perform all the requested risk assessments before releasing any GM product and next post-release checking to track probable gene flow and limit any possible contamination of the food chain catastrophe. Therefore, the safe use of this technology, in compliance with all protocols of environmental health and safety assessment at the national and international levels are demanded.
转基因技术可以通过增强对害虫和除草剂的抵抗力、耐旱性、催熟性,并最终提高产量和食品质量,克服食品工业中的农业和营养困难、食品安全和保障。然而,在过去的几十年里,转基因食品对人类、动物和环境的重大危险已经被发现。然而,没有足够的科学证据证明这些食物对人类和动物健康的有害影响。本文综述了该技术的几个优点和缺点。因此,有必要在发布任何转基因产品之前进行所有要求的风险评估,并在下一次发布后进行检查,以跟踪可能的基因流动,并限制食品链灾难的任何可能污染。因此,要求安全使用这项技术,遵守国家和国际层面的所有环境健康和安全评估协议。
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引用次数: 0
Molecular surveillance of non-O157 Shiga toxigenic Escherichia coli in selected chicken abattoirs and retail outlets in Osogbo, Osun State, Nigeria 尼日利亚奥孙州Osogbo选定鸡屠宰场和零售店非o157志贺毒素大肠杆菌的分子监测
Pub Date : 2022-07-03 DOI: 10.18502/jfsh.v8i1.9957
F. Ayoade, J. Oguzie, K. Akano, T. Olumade, Philomena J. Eromon, Kanyinsola Odesola, O. Folarin, C. Happi
Three selected chicken abattoirs and two retail locations were studied to determine the frequency of occurrence and profile for Shiga toxin-producing Escherichia coli (STEC) strains present in abattoirs and retail (frozen) chicken carcasses in Osun state, Nigeria. Samples were plated on Eosin Methylene Blue agar for the presence of E. coli. Furthermore, the isolates were confirmed serologically as non-O157 STEC using latex agglutination serotyping kit. Multiplex PCR was used to check for specific virulence factors in the isolated E. coli strains. The mean colony count results showed that effluent water samples from the Ikirun slaughter slab type abattoir were the highest at 25 cfu/ml. A post hoc comparison showed that this value was significantly higher than that of the slaughtering table at Oluode-1 (P = 0.04) and retail chicken meat samples at Igbona (P = 0.01). The results show that chicken abattoirs are poor reservoirs of STEC. Moreover, the results from this study showing that the stx2-producing strains that are more prone to cause hemolytic uremic syndrome is the predominant strain in the study area is worrisome. These results underscore the improper hygiene practices of the abattoir workers combined with inadequate waste management and biological waste disposal systems. It is recommended that regulatory bodies in this locality should focus on ensuring the upgrade of biological waste disposal from these abattoirs in order to limit spread of potentially virulent pathogens into the runoff and ground water.
研究了选定的三个鸡屠宰场和两个零售地点,以确定尼日利亚奥松州屠宰场和零售(冷冻)鸡尸体中存在的产志贺毒素大肠杆菌(STEC)菌株的发生频率和概况。样品被镀在伊红亚甲基蓝琼脂上检测大肠杆菌的存在。此外,使用乳胶凝集血清分型试剂盒对分离株进行血清学鉴定,证实其为非o157产志在大肠杆菌。采用多重PCR检测分离的大肠杆菌菌株的特异性毒力因子。平均菌落计数结果显示,Ikirun屠宰板型屠宰场的出水样品最高,为25 cfu/ml。事后比较表明,该值显著高于Oluode-1屠宰场(P = 0.04)和Igbona零售鸡肉样品(P = 0.01)。结果表明,鸡屠宰场是产志毒素大肠杆菌的不良宿主。此外,本研究结果显示更容易引起溶血性尿毒症综合征的产stx2菌株是研究地区的优势菌株,这令人担忧。这些结果强调了屠宰场工人不适当的卫生习惯以及不充分的废物管理和生物废物处理系统。建议当地的管理机构应侧重于确保这些屠宰场的生物废物处理的升级,以限制潜在的致命病原体向径流和地下水的传播。
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引用次数: 0
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Journal of food safety and hygiene
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