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Prevalence of antibiotic resistant pathogenic E. coli from animals, retail and humans diagnosed with Gastroenteritisv 动物、零售业和人类诊断为胃肠病学的抗生素耐药性致病性大肠杆菌的患病率
Pub Date : 2021-06-23 DOI: 10.18502/jfsh.v6i2.6521
A. Gomez, Elizabeth Aguilera Nunez, P. McDonough, Yungfu Chang, H. Mohammed
Foodborne pathogens represent one of the major challenges to health systems around the world. This risk is exacerbated by the presence of antimicrobial resistant (AMR) pathogens. Knowledge of the presence of these pathogens in the food supply chain would help in establishing intervention strategies to mitigate their risk. The objective of this study was to detect AMR among serotypes of Escherichia coli (E. coli) food adulterants serotypes of E. coli in the food supply chain and among isolates from gastroenteritis cases. E. coli isolates recovered from animals, meat processing plants, retail, and humans were examined for the presence of AMR using phenotypic and genotypic approaches. AMR to aminoglycosides, β-lactams, and tetracycline were detected in all isolates recovered from these sources at different levels. Similarly, presence of the bla-Tem, bla-SHV, aadA, and strAB genes were detected in isolates from these sources but there was no significant correlation between the genetic detection and phenotypic expression AMR.
食源性病原体是世界各地卫生系统面临的主要挑战之一。耐药性病原体的存在加剧了这种风险。了解这些病原体在食品供应链中的存在将有助于制定干预策略来降低其风险。本研究的目的是检测食品供应链中大肠杆菌血清型和胃肠炎分离株中的AMR。使用表型和基因型方法检测从动物、肉类加工厂、零售店和人类中回收的大肠杆菌分离株是否存在AMR。从这些来源回收的所有分离株中都检测到不同水平的氨基糖苷类、β-内酰胺类和四环素的AMR。类似地,在来自这些来源的分离株中检测到bla-Tem、bla-SHV、aadA和strAB基因的存在,但遗传检测和表型表达AMR之间没有显著相关性。
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引用次数: 0
Multidrug resistant Salmonella spp. and Escherichia coli from a popular Ready-to-Eat local food (Fufu) from commercial food vendors in Ghana 耐多药沙门氏菌和大肠杆菌来自加纳商业食品供应商的一种流行的即食当地食品(福福)
Pub Date : 2021-03-10 DOI: 10.18502/JFSH.V5I4.5702
C. Saba, A. Karikari, Enoch Yeleliere, Patrick Takyi, S. W. Kpordze
Microbial contamination of vended foods are of public health importance due to the potential of becoming a reservoir of foodborne pathogens and resistant strains of bacteria. This study looked at the presence of pathogenic bacteria in a popular Ready-To-Eat (RTE) traditional food, Fufu in Ghana. Sixty (60) Fufu samples were obtained from various food joints categorized as Opened, Semi-closed and Closed or Restaurants. Samples were processed and analyzed using standard bacteriological methods. The susceptibility profiles of the isolates were obtained by using the Kirby-Bauer disk diffusion method with the EUCAST guidelines with the five antibiotics. Prevalence of E. coli was 85% and Salmonella species was 68%. Microbial count of isolated E. coli ranged from 0 to 3×106 cfu/ml. There were no significant differences (p>0.05) among the different modes of operations. Fufu samples from Opened, Semi-closed and Closed joints were respectively contaminated with E. coli and Salmonella species as follows: 92%, 76%; 80%, 60% and 80%, 65%. The Salmonella species showed highest resistance to erythromycin (58.5%) and E. coli species were commonly resistant to Ceftazidime (88.2%) and Ceftriaxone (94.1%). All isolates were susceptible to nitrofurantoin. Multidrug resistance was detected among 27.5% of E. coli strains and 14.6% of Salmonella species. Fufu from the different eating joints in the Tamale Metropolis were substantially contaminated with multidrug resistant pathogens. The study recommends surveillance studies of resistant pathogens in foods, increased education and training of food vendors on sanitation, food handling and safety practices in the region.
由于有可能成为食源性病原体和耐药菌株的宿主,供应食品的微生物污染对公共卫生具有重要意义。这项研究观察了加纳一种流行的即食食品福福中的致病菌。六十(60)份福府样品来自不同的餐饮场所,分为开放式、半封闭式和封闭式或餐厅。使用标准细菌学方法对样品进行处理和分析。使用Kirby-Bauer圆盘扩散法和EUCAST指南对五种抗生素获得分离株的易感性谱。大肠杆菌的患病率为85%,沙门氏菌为68%。分离的大肠杆菌的微生物计数范围为0-3×106cfu/ml。不同手术方式间差异无统计学意义(p>0.05)。福福公司的开放式、半封闭式和封闭式接头样品分别被大肠杆菌和沙门氏菌污染,污染率分别为92%、76%;80%、60%和80%、65%。沙门氏菌对红霉素的耐药性最高(58.5%),大肠杆菌对头孢他啶和头孢曲松的耐药性一般(88.2%)。27.5%的大肠杆菌和14.6%的沙门氏菌对多种药物产生耐药性。来自塔马勒大都会不同饮食场所的福府被多药耐药性病原体严重污染。该研究建议对食品中的耐药性病原体进行监测研究,加强对该地区食品供应商的卫生、食品处理和安全做法的教育和培训。
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引用次数: 2
Antioxidant properties of traditional sour cherry juice in light and dark conditions at ambient temperature 常温下传统酸樱桃汁在明暗条件下的抗氧化性能
Pub Date : 2021-03-10 DOI: 10.18502/JFSH.V5I3.5695
Mohadeseh Pirhadi, P. Sadighara, A. Mohajer, G. J. Khaniki
Sour cherry is a delicious horticultural product, which has been widely developed due to the richness of valuable antioxidant and phenolic nutritional properties in the world. This research was conducted to investigate the quantitative properties of phenolic compounds and antioxidant activity of cherry fruit and compare it in 8 days with light and dark conditions. In this experimental study, cherries were purchased from Tehran fruit market randomly, and they were transferred to the food analysis laboratory. Subsequently, the cherry extract was taken via juicer.Total antioxidant capacity (TAC) )and total flavonoid content (TFC ) Cherry juice were measured with a UV-vis spectrophotometer every 24 h during 8 days. Data analysis was carried out using SPSS software. The results of the current study showed that the amount of antioxidant compounds of cherry extract in brightness and darkness was 1.96 mg/kg and 2.13 mg/kg, respectively, and the flavonoid composition of light and dark condition was 0.15 mg/kg and 0.16 mg/kg, respectively. Light can reduce the antioxidant and flavonoid properties of sour cherry juice. The antioxidant and flavonoid properties of traditional sour cherry juice extracts are sensitive to light conditions.
酸樱桃是一种美味的园艺产品,由于其丰富的抗氧化和酚类营养特性,在世界范围内得到了广泛的开发。本研究旨在研究樱桃果实中酚类化合物的定量性质和抗氧化活性,并将其在光照和黑暗条件下8天进行比较。在这项实验研究中,樱桃是从德黑兰水果市场随机购买的,并被转移到食品分析实验室。随后,通过榨汁机提取樱桃提取物。总抗氧化能力(TAC))和总黄酮含量(TFC)用紫外-可见分光光度计在8天内每24小时测量一次樱桃汁。数据分析采用SPSS软件进行。本研究结果表明,樱桃提取物在明亮和黑暗条件下的抗氧化化合物含量分别为1.96 mg/kg和2.13 mg/kg,在明亮和昏暗条件下的类黄酮组成分别为0.15 mg/kg和0.16 mg/kg。光照会降低酸樱桃汁的抗氧化和类黄酮特性。传统酸樱桃汁提取物的抗氧化和类黄酮特性对光照条件敏感。
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引用次数: 0
Effect of soaking, boiling and frying on selenium content of major african fresh foods. 浸泡、煮沸和油炸对非洲主要新鲜食品硒含量的影响。
Pub Date : 2021-01-01 DOI: 10.18502/jfsh.v7i4.9283
Lawrence Fred Sembajwe, Joshua Nfambi, Allan Lugaajju, Robert Kalyesubula

Selenium is an essential trace element that is crucial for normal functioning of human body systems and it is obtained from diet or dietary supplements. The concentration of selenium (Se) varies from soil-to-soil and therefore in various food types. We profiled the selenium concentration in the major African fresh foods and studied the effect of boiling, frying and overnight soaking on their selenium content. The foods were taken to the chemistry laboratory, processed for determining selenium concentration in the fresh foods and then subjected to the various treatments before determining their Se concentration using an atomic absorption spectrometer. Among the fresh foods studied, pumpkin seeds were found to have the highest Se concentration (109.25 ± 0.125 parts per billion (ppb)), while Irish potatoes had the lowest (16.25 ± 0.125 ppb). In most fresh foods studied, boiling and frying had the effect of reducing the Se concentration, except in yams that showed an increased concentration after frying. Overnight soaking of matooke (plantain bananas) and beans showed an effect of causing a considerable reduction in the Se concentration from 36.375 ± 0.281 ppb and 59.125 ± 0.031 ppb to 14.03 ± 0.441 ppb and 24.375 ± 0.281 ppb, respectively. Boiling and frying cause a significant reduction in Se concentration of most African fresh foods. Overnight soaking of matooke and beans caused further reduction in the selenium concentration of the fresh foods. This implies that people who pre-soak their food before cooking it are likely to lose more selenium than those who do not soak and may be more likely to suffer from Se deficiencies.

硒是一种必需的微量元素,对人体系统的正常运作至关重要,它可以从饮食或膳食补充剂中获得。硒(Se)的浓度因土壤而异,因此在不同的食物类型中也不同。对非洲主要新鲜食品中的硒含量进行了分析,研究了煮沸、油炸和隔夜浸泡对其硒含量的影响。这些食品被送到化学实验室,进行加工以测定新鲜食品中的硒浓度,然后进行各种处理,然后用原子吸收光谱仪测定其硒浓度。在研究的新鲜食品中,南瓜籽的硒含量最高(109.25±0.125 ppb),而爱尔兰土豆的硒含量最低(16.25±0.125 ppb)。在大多数被研究的新鲜食品中,煮沸和油炸都有降低硒浓度的效果,除了油炸后浓度增加的山药。大蕉和蚕豆浸泡过夜后,硒浓度分别从36.375±0.281 ppb和59.125±0.031 ppb降至14.03±0.441 ppb和24.375±0.281 ppb。煮沸和油炸导致大多数非洲新鲜食品的硒浓度显著降低。隔夜浸泡的麻豆和豆类导致新鲜食品中的硒浓度进一步降低。这意味着在烹饪前预先浸泡食物的人比不浸泡食物的人更容易失去更多的硒,更容易患硒缺乏症。
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引用次数: 0
Current status of mycotoxigenic fungi in cereal grains in the central region of Botswana: a mini survey 博茨瓦纳中部地区谷物中真菌毒素真菌的现状:一项小型调查
Pub Date : 2020-08-10 DOI: 10.18502/jfsh.v5i2.3950
Basadi Masitha, Bokani Sereme-Mothobole, Kago Kabelo
Mycotoxins are secondary metabolites produced by fungi that can contaminate food, both human and animal feed at all stages of the food chain. A number of factors play a role in the proliferation of mycotoxins such as climate, humidity, harvest and storage conditions. This study was looking at the occurrence and identification of the fungi obtained from the cereal grains in the central district of Botswana. Samples collected were yellow maize (18), white maize (4), millet (10), cowpeas (11), sorghum (11) and china peas (1) each weighing about 500 g. Upon the arrival of samples, water activities of the samples were obtained. Seeds were sterilized in sodium hypochlorite, to be plated onto PDA for fungal extraction. The polymerase Chain reaction was used for the identification of the fungi and samples were sent to Inqaba laboratories for sequencing. The results showed that yellow maize was contaminated by Fusarium, A. niger and A. flavus; white maize was contaminated by F. proliferatum, F. fujikuroi and Gibberella moniliformis; red sorghum was contaminated by A. flavus, A.oryzae, Penicillium, Alternaria and Chaetomium muelleri; millet was contaminated by Epicoccum sorghinum and curvularia branchyspora and cowpeas were contaminated by Aspergillus and Alternaria species. Overall the most contaminated cereal product was millet, yellow maize, white maize, cowpeas and red sorghum at 40%, 37%, 27%, 10% and 4% respectively.
真菌毒素是真菌产生的次生代谢物,可在食物链的所有阶段污染食物,包括人类和动物饲料。许多因素在真菌毒素的增殖中起作用,如气候、湿度、收获和储存条件。这项研究着眼于从博茨瓦纳中部地区的谷物中获得的真菌的发生和鉴定。采集的样品为黄玉米(18个)、白玉米(4个)、小米(10个)、豇豆(11个)、高粱(11个)和中国豌豆(1个),各重约500克。样品到达后,得到样品的水活度。种子在次氯酸钠中灭菌,然后镀在PDA上进行真菌提取。采用聚合酶链反应对真菌进行鉴定,并将样品送往因卡巴实验室进行测序。结果表明,黄玉米受镰刀菌、黑曲霉和黄曲霉污染;白玉米被增殖镰刀菌、藤黑镰刀菌和念珠赤霉素污染;红高粱被黄曲霉、米霉、青霉、交替菌和毛霉污染;谷子受高粱表皮霉和弯孢霉污染,豇豆受曲霉和Alternaria污染。总体而言,受污染最严重的谷类产品是小米、黄玉米、白玉米、豇豆和红高粱,分别为40%、37%、27%、10%和4%。
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引用次数: 1
A study on milk quality testing and awareness of science and non science students on milk adulteration 牛奶质量检测及理科生与非理科生对牛奶掺假意识的研究
Pub Date : 2020-08-10 DOI: 10.18502/jfsh.v5i2.3944
Sadath Fatima, A. Vasundhara
Increased global demand for milk has made it prone to massive levels of adulteration. The aims of this study were to analyze the quality of milk samples and also to assess the awareness of science and non-science students on milk adulteration. A total of 100 milk samples [unbranded (n=50) and branded (n=50)] were randomly collected from different areas of Hyderabad and were chemically analyzed to detect the presence of adulterants using standard procedures. A pre-tested questionnaire was used to assess the knowledge of students (sciences and non-sciences) on milk adulteration. It was observed from the chemical analysis that the unbranded milk samples (n=50) and branded milk samples (n=50) were found to be adulterated with water, cane sugar, urea, detergent, skimmed milk powder and formalin with their extent being 82%, 24%, 12%, 36%, 26%, 22% and 6%, 2%, 0%, 0%, 36%, 28% respectively. It was found from a microbiological examination that 92% of branded milk samples were in good quality, 2% were in fair quality and 6% were in poor quality and all the unbranded milk samples were found to be of poor quality. A significant difference (p<0.05) was observed on the awareness of milk adulteration between Science and Non-Science student respondents. It can be concluded that milk quality is not as per standards. To eradicate this Malpractice, having a quality control system is necessary which could regularly check to ensure good quality milk is sold.
全球对牛奶需求的增加使其容易出现大量掺假现象。本研究的目的是分析牛奶样品的质量,并评估科学和非科学学生对牛奶掺假的认识。从海得拉巴的不同地区随机收集了总共100个牛奶样本[无品牌(n=50)和品牌(n=50],并使用标准程序进行化学分析以检测是否存在掺杂物。使用预先测试的问卷来评估学生(科学和非科学)对牛奶掺假的知识。化学分析结果表明,无品牌奶(n=50)和品牌奶(n=50)分别掺假水、蔗糖、尿素、洗涤剂、脱脂奶粉和福尔马林,掺假程度分别为82%、24%、12%、36%、26%、22%和6%、2%、0%、36%、36%、28%。微生物检查发现,92%的品牌牛奶样品质量良好,2%的质量尚可,6%的质量较差,所有未品牌牛奶样品都质量较差。理科和非理科学生在牛奶掺假意识方面存在显著差异(p<0.05)。可以得出结论,牛奶质量不符合标准。为了根除这种不良行为,有必要建立一个质量控制系统,定期检查以确保销售质量良好的牛奶。
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引用次数: 0
Assessment of the microbiological quality of beverages sold in collective cafes on the campuses of the University of Abomey-Calavi, Benin Republic 贝宁共和国阿博美-卡拉维大学校园内集体咖啡馆销售的饮料微生物质量评估
Pub Date : 2020-08-10 DOI: 10.18502/jfsh.v5i2.3948
Gwladys S. Komagbe, P. Sessou, François S. P. Dossa, Prudencio Sossa-Minou, B. Taminiau, P. Azokpota, N. Korsak, G. Daube, S. Farougou
Fresh juices are highly nutritious foods for human beings, but the inability to observe requirements for their preparation, packaging and storage subjects them to microbial contamination which poses a potential health risk to consumers. The purpose of this study was to evaluate the microbiological quality of beverages sold within the cafes of the campuses of Abomey-Calavi University (Benin). A survey carried out among beverage vendors showed that the sources of contamination were uncontrolled and the raw materials used were of questionable quality as the operators lacked good hygienic practices. Thus, the microbial quality of forty-five samples of four types of beverages sold in these cafes was investigated for mesophilic aerobic flora, Coliforms, Escherichia coli, Staphylococcus aureus, sulfate-reducing anaerobic spores, fungal flora and Salmonella spp. using standardized methods. Then, molecular studies identified the pathogenic strains isolated from the beverages. An antibiotic susceptibility test was performed on the strains identified for the detection of multi-resistant bacteria. These analyses revealed a non-compliance rate of 100% in the analyzed samples. The indicators that caused this non-compliance in the samples were mesophilic aerobic flora, coliforms and fungi. In addition, 85.7% of the samples contained other Enterobacteriaceae including Klebsiella pneumoniae, Morganella morganii, Kluyvera georgiana, Citrobacter murliniae, Yersinia intermedia. While the non-compliance rates of the samples for Salmonella spp and E.coli were 4.4% each, the non-compliance rate for S. aureus was 2.2% with the presence of sometimes multi-resistant pathogenic bacteria. Sellers' awareness of good hygiene practices is important for improving the quality of food sold.
新鲜果汁对人类来说是营养丰富的食物,但由于无法遵守其制备、包装和储存要求,它们会受到微生物污染,这对消费者的健康构成潜在风险。本研究的目的是评估Abomey Calavi大学(贝宁)校园咖啡馆内出售的饮料的微生物质量。对饮料供应商进行的一项调查显示,由于经营者缺乏良好的卫生习惯,污染源不受控制,使用的原材料质量可疑。因此,使用标准化方法对这些咖啡馆出售的四种饮料的45个样品的微生物质量进行了调查,包括嗜中温需氧菌群、大肠杆菌、金黄色葡萄球菌、硫酸盐还原厌氧孢子、真菌菌群和沙门氏菌属。然后,分子研究确定了从饮料中分离出的致病菌株。对已鉴定的菌株进行抗生素敏感性试验,以检测多重耐药细菌。这些分析表明,所分析的样本中不符合要求的比率为100%。导致样品不符合要求的指标是中温需氧菌群、大肠菌群和真菌。此外,85.7%的样本含有其他肠杆菌科细菌,包括肺炎克雷伯菌、摩根氏Morganella morganii、格鲁吉亚Kluyvera、murliniae柠檬酸杆菌、中间耶尔森菌。虽然沙门氏菌和大肠杆菌样本的不符合率分别为4.4%,但金黄色葡萄球菌的不符合比率为2.2%,有时存在多重耐药性致病菌。销售者对良好卫生习惯的认识对于提高所售食品的质量很重要。
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引用次数: 1
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Journal of food safety and hygiene
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