Pub Date : 2024-02-24DOI: 10.26420/austinjanalpharmchem.2024.1168
Santosh Mallikarjun Bhav, Shubha K Mirji, Bothe Thokchom, Sapam Riches Singh, Raju B. Maliger, Shivanand S Bhat, Pooja Joshi, BP Harini, Ramesh Babu Yarajarla, Salim Al Jadidi
This study explores the use of Syzygium cumini (L.) Skeels plant aqueous leaf extract as a reducing agent, which enables the green synthesis and comprehensive characterization of silver nanoparticles (AgNPs). The results indicated that the synthesized AgNPs were spherical in shape with an average size of 27.5 nm and a silver content of approximately 43.18%. The AgNPs exhibited promising antidiabetic and wound-healing properties. The antidiabetic activity, measured through glucose uptake and α-amylase inhibition assays, showed values of 80.08% and 83.91%, respectively. Additionally, the cytotoxicity assessment revealed that the AgNPs exhibited good biocompatibility even at higher doses, indicating their lower toxicity profile. Furthermore, the AgNPs demonstrated wound-closure percentages of 27.59% and 92.48% at 12 h and 24 h respectively, post-treatment, indicating that they were as effective as that of the standard ascorbic acid. These findings suggest that Syzygium cumini-mediated AgNPs have significant potential for applications in antidiabetic and wound-healing treatments.
{"title":"Potential Antidiabetic Properties of Syzygium Cumini (L.) Skeels Leaf Extract-Mediated Silver Nanoparticles","authors":"Santosh Mallikarjun Bhav, Shubha K Mirji, Bothe Thokchom, Sapam Riches Singh, Raju B. Maliger, Shivanand S Bhat, Pooja Joshi, BP Harini, Ramesh Babu Yarajarla, Salim Al Jadidi","doi":"10.26420/austinjanalpharmchem.2024.1168","DOIUrl":"https://doi.org/10.26420/austinjanalpharmchem.2024.1168","url":null,"abstract":"This study explores the use of Syzygium cumini (L.) Skeels plant aqueous leaf extract as a reducing agent, which enables the green synthesis and comprehensive characterization of silver nanoparticles (AgNPs). The results indicated that the synthesized AgNPs were spherical in shape with an average size of 27.5 nm and a silver content of approximately 43.18%. The AgNPs exhibited promising antidiabetic and wound-healing properties. The antidiabetic activity, measured through glucose uptake and α-amylase inhibition assays, showed values of 80.08% and 83.91%, respectively. Additionally, the cytotoxicity assessment revealed that the AgNPs exhibited good biocompatibility even at higher doses, indicating their lower toxicity profile. Furthermore, the AgNPs demonstrated wound-closure percentages of 27.59% and 92.48% at 12 h and 24 h respectively, post-treatment, indicating that they were as effective as that of the standard ascorbic acid. These findings suggest that Syzygium cumini-mediated AgNPs have significant potential for applications in antidiabetic and wound-healing treatments.","PeriodicalId":91055,"journal":{"name":"Austin journal of analytical and pharmaceutical chemistry","volume":"9 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140434450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-13DOI: 10.26420/austinjanalpharmchem.2023.1155
Jean-Marc Sabatier, Farzan Amini
The purpose of this article is to study about the Coupling of Gloomy Eyelets inside the nucleus of cells. The Gloomy Eyelet consists of the nano distortion of space-time (with υi and τi denote volume and time) and the Latent Buoyancy force. The particular Helmholtz resonator (for υi and τi) can define for the distortion of space-time (for υi and τi) of Gloomy Eyelet. The Hamiltonian equation will be applied for the category of nucleus of cells including the numerous Gloomy Eyelets. The coupled Gloomy Eyelets can build the virtual mass. The virtual mass can be considered as the Unknown Distinct Structure (UDS) of human body organs and performs a unknown specific function. If failure occurs in one of the Unknown Distinct Structure (UDS), the conventional treatments may be less effective for ones. The UDS can be affected by the variation of gravitational, ultrasonic and radiation fields. The internal and external interactions of UDS can play a key role for human beings healthy. The Unstable and Transient conditions of Gloomy Eyelet of cell nucleus can be considered as a new approach for the study of the disturbance of cell.
{"title":"Coupling Gloomy Eyelets in the Nucleus of Cells and Forming Unknown Distinct Structure (UDS)","authors":"Jean-Marc Sabatier, Farzan Amini","doi":"10.26420/austinjanalpharmchem.2023.1155","DOIUrl":"https://doi.org/10.26420/austinjanalpharmchem.2023.1155","url":null,"abstract":"The purpose of this article is to study about the Coupling of Gloomy Eyelets inside the nucleus of cells. The Gloomy Eyelet consists of the nano distortion of space-time (with υi and τi denote volume and time) and the Latent Buoyancy force. The particular Helmholtz resonator (for υi and τi) can define for the distortion of space-time (for υi and τi) of Gloomy Eyelet. The Hamiltonian equation will be applied for the category of nucleus of cells including the numerous Gloomy Eyelets. The coupled Gloomy Eyelets can build the virtual mass. The virtual mass can be considered as the Unknown Distinct Structure (UDS) of human body organs and performs a unknown specific function. If failure occurs in one of the Unknown Distinct Structure (UDS), the conventional treatments may be less effective for ones. The UDS can be affected by the variation of gravitational, ultrasonic and radiation fields. The internal and external interactions of UDS can play a key role for human beings healthy. The Unstable and Transient conditions of Gloomy Eyelet of cell nucleus can be considered as a new approach for the study of the disturbance of cell.","PeriodicalId":91055,"journal":{"name":"Austin journal of analytical and pharmaceutical chemistry","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139370175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Current fundamental researches in the field of potentiometry demonstrate the perspectives of using polymeric nanostructured materials as ion-to-electron transducers in Solid-Contact Potentiometric Sensors (SCSs). This paper reports the preparation and comparative study of plasticized Poly(Vinyl Chloride) (PVC) membranes modified with fullerene C60, single-walled carbon nanotubes SWCNTs, multiwalled carbon nanotubes, and graphene oxide as the transducing layer in SCSs for the determination of a common local anesthetic drug – procaine hydrochloride (Pro·HCl). As an ion-sensitive (recognizing) layer, a PVC membrane containing highly lipophilic 2-[bis-octadecyl sulfo]-closo-decaborate anions was chosen. The results were discussed in relation to nanofiller’s type and content. The best potentiometric characteristics were obtained for the sensor with the transducing layer containing the hybrid nanofiller SWCNTs/C60. This new sensor of a double-layer PVC membrane configuration exhibited a Nernstian slope (59.2 ± 0.2 mV/decade) in the wide linear range (5×10-7– 1×10-2 M) with the low limit of detection (10-7.1 M), fast response time (≤ 7 s), and stable potentiometric response (drift potential ± 0.27 mV h-1 over 7 h of soaking in 1x10-5 M Pro·HCl solution).
{"title":"Development of Solid-Contact Potentiometric Sensor Utilized Nanocarbon-filled Poly (vinyl chloride) Membrane as Ion-to-electron Transducing Layer","authors":"Turyshev Es, Shpigun Lk, Kopytin Av, Zhizhin Ky, Kuznetsov Nt, Simonenko Np","doi":"10.26420/austinjanalpharmchem.2023.1154","DOIUrl":"https://doi.org/10.26420/austinjanalpharmchem.2023.1154","url":null,"abstract":"Current fundamental researches in the field of potentiometry demonstrate the perspectives of using polymeric nanostructured materials as ion-to-electron transducers in Solid-Contact Potentiometric Sensors (SCSs). This paper reports the preparation and comparative study of plasticized Poly(Vinyl Chloride) (PVC) membranes modified with fullerene C60, single-walled carbon nanotubes SWCNTs, multiwalled carbon nanotubes, and graphene oxide as the transducing layer in SCSs for the determination of a common local anesthetic drug – procaine hydrochloride (Pro·HCl). As an ion-sensitive (recognizing) layer, a PVC membrane containing highly lipophilic 2-[bis-octadecyl sulfo]-closo-decaborate anions was chosen. The results were discussed in relation to nanofiller’s type and content. The best potentiometric characteristics were obtained for the sensor with the transducing layer containing the hybrid nanofiller SWCNTs/C60. This new sensor of a double-layer PVC membrane configuration exhibited a Nernstian slope (59.2 ± 0.2 mV/decade) in the wide linear range (5×10-7– 1×10-2 M) with the low limit of detection (10-7.1 M), fast response time (≤ 7 s), and stable potentiometric response (drift potential ± 0.27 mV h-1 over 7 h of soaking in 1x10-5 M Pro·HCl solution).","PeriodicalId":91055,"journal":{"name":"Austin journal of analytical and pharmaceutical chemistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44538201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-17DOI: 10.26420/austinjanalpharmchem.2023.1153
Sarika Chaudhary
The present research work involves the synthesis of a total of four Curcumin-NSAIDs conjugates and their subsequent biological screening for Rheumatoid Arthritis, Anti-inflammatory, and Anti-ulcer activities. Curcumin is a phytochemical having versatile therapeutic potential. The idea of conjugating curcumin with various NSAIDs is somewhat novel in the sense that it helps in obtaining the target of incorporating certain desired properties in the curcumin like enhanced bioavailability, reduction in the side effects and also giving good therapeutic potential in terms of determination of rheumatoid arthritis, anti-inflammatory and anti-ulcer activities of synthesized conjugates using appropriate animal models. Additionally, studies have been done on the in vivo bioavailability of curcumin-NSAIDs conjugates and curcumin in Sprague-Dawley rats and on the antiarthritic efficacy of curcumin-NSAIDs conjugates and curcumin in a modified streptococcal cell wall-induced arthritis model in Balb/c mice to resemble rheumatoid arthritis in human. Curcumin-NSAID conjugates have shown improved stability in all of the studies mentioned when compared to curcumin and curcumin-NSAID conjugates; they also increased curcumin’s bioavailability by more than five folds and significantly reduced arthritis symptoms in a streptococcal cell wall-induced arthritis model when compared to other conjugates and curcumin. The structural features of all the synthesized compounds have been confirmed by appropriate spectral analysis. Other supporting techniques employed for structural and purity aspects include Melting point determination, Thin Layer Chromatography analysis, and Recrystallization. Therefore, conjugating curcumin with medicinal molecules proved to be a creative way to increase its bioavailability and enhance its wide range of pharmacological effects.
{"title":"Synthesis and Biological Evaluation of Curcumin-NSAIDs Conjugates","authors":"Sarika Chaudhary","doi":"10.26420/austinjanalpharmchem.2023.1153","DOIUrl":"https://doi.org/10.26420/austinjanalpharmchem.2023.1153","url":null,"abstract":"The present research work involves the synthesis of a total of four Curcumin-NSAIDs conjugates and their subsequent biological screening for Rheumatoid Arthritis, Anti-inflammatory, and Anti-ulcer activities. Curcumin is a phytochemical having versatile therapeutic potential. The idea of conjugating curcumin with various NSAIDs is somewhat novel in the sense that it helps in obtaining the target of incorporating certain desired properties in the curcumin like enhanced bioavailability, reduction in the side effects and also giving good therapeutic potential in terms of determination of rheumatoid arthritis, anti-inflammatory and anti-ulcer activities of synthesized conjugates using appropriate animal models. Additionally, studies have been done on the in vivo bioavailability of curcumin-NSAIDs conjugates and curcumin in Sprague-Dawley rats and on the antiarthritic efficacy of curcumin-NSAIDs conjugates and curcumin in a modified streptococcal cell wall-induced arthritis model in Balb/c mice to resemble rheumatoid arthritis in human. Curcumin-NSAID conjugates have shown improved stability in all of the studies mentioned when compared to curcumin and curcumin-NSAID conjugates; they also increased curcumin’s bioavailability by more than five folds and significantly reduced arthritis symptoms in a streptococcal cell wall-induced arthritis model when compared to other conjugates and curcumin. The structural features of all the synthesized compounds have been confirmed by appropriate spectral analysis. Other supporting techniques employed for structural and purity aspects include Melting point determination, Thin Layer Chromatography analysis, and Recrystallization. Therefore, conjugating curcumin with medicinal molecules proved to be a creative way to increase its bioavailability and enhance its wide range of pharmacological effects.","PeriodicalId":91055,"journal":{"name":"Austin journal of analytical and pharmaceutical chemistry","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41773343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-29DOI: 10.26420/austinjanalpharmchem.2022.1152
Yixin Huang
Cystatin C (Cys-C) is a protease inhibitor that can be produced by nuclear cells in the human body. Its principal function is to inhibit the activity of various cysteine proteases in vivo. In addition, Cys-C also plays an essential role in regulating antigen presentation, extracellular matrix degradation, and the balance of protein production and decomposition in living cells. Cys-C is involved in the pathophysiological processes of chronic inflammatory diseases, including cardiovascular, cerebrovascular, and respiratory diseases. This article reviews the effects of Cys-C on chronic inflammatory diseases and its therapeutic prospects.
{"title":"An Overview about the Relationship between Cystatin C and Inflammatory Diseases","authors":"Yixin Huang","doi":"10.26420/austinjanalpharmchem.2022.1152","DOIUrl":"https://doi.org/10.26420/austinjanalpharmchem.2022.1152","url":null,"abstract":"Cystatin C (Cys-C) is a protease inhibitor that can be produced by nuclear cells in the human body. Its principal function is to inhibit the activity of various cysteine proteases in vivo. In addition, Cys-C also plays an essential role in regulating antigen presentation, extracellular matrix degradation, and the balance of protein production and decomposition in living cells. Cys-C is involved in the pathophysiological processes of chronic inflammatory diseases, including cardiovascular, cerebrovascular, and respiratory diseases. This article reviews the effects of Cys-C on chronic inflammatory diseases and its therapeutic prospects.","PeriodicalId":91055,"journal":{"name":"Austin journal of analytical and pharmaceutical chemistry","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42706273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-15DOI: 10.26420/austinjanalpharmchem.2022.1150
Anshuman Srivastava, M. Godbole, Ashutosh Shrivastava
Steroid profiling plays an important role in the clinical setting and also in the diagnosis of various physiological disorders. Steroids in the research laboratory and patient care has been routinely measured by immunoassay. Limitations of immunoassay in quantifying steroids have been well documented and the advent of advanced mass spectrometry is offering a viable alternative to measure multiple steroids in single reaction. Analytes ranging from the steroid and their metabolites that are present in the body carry out various important functions and are relevant to maintaining homeostasis. Blood plasma and urine samples are the clinical material for such analysis, however, due to the difference in polarity of the target steroid, a major challenge exists as to how faithfully analyze different steroids in the given sample type. These steroids are usually present at very low concentrations in the body and are present in several forms leading to increased complexity. Achieving an excellent chromatographic separation for the analysis of steroids requires an effective sample preparation and analysis procedure. Hence various methods are developed to analyze the presence of steroids and to know their exact concentration in the body. This review covers various analytical methodologies such as chromatographic procedures and downstream mass spectrometry for the identification and measurement of the levels of steroid hormones. At the same time, this review also covers the most recent developments that have taken place in the recent past to cover the enhanced understanding of steroid hormone analysis.
{"title":"Estimation of Steroid Hormones in Biological Samples Using Micro Extraction and Advanced Chromatography Techniques","authors":"Anshuman Srivastava, M. Godbole, Ashutosh Shrivastava","doi":"10.26420/austinjanalpharmchem.2022.1150","DOIUrl":"https://doi.org/10.26420/austinjanalpharmchem.2022.1150","url":null,"abstract":"Steroid profiling plays an important role in the clinical setting and also in the diagnosis of various physiological disorders. Steroids in the research laboratory and patient care has been routinely measured by immunoassay. Limitations of immunoassay in quantifying steroids have been well documented and the advent of advanced mass spectrometry is offering a viable alternative to measure multiple steroids in single reaction. Analytes ranging from the steroid and their metabolites that are present in the body carry out various important functions and are relevant to maintaining homeostasis. Blood plasma and urine samples are the clinical material for such analysis, however, due to the difference in polarity of the target steroid, a major challenge exists as to how faithfully analyze different steroids in the given sample type. These steroids are usually present at very low concentrations in the body and are present in several forms leading to increased complexity. Achieving an excellent chromatographic separation for the analysis of steroids requires an effective sample preparation and analysis procedure. Hence various methods are developed to analyze the presence of steroids and to know their exact concentration in the body. This review covers various analytical methodologies such as chromatographic procedures and downstream mass spectrometry for the identification and measurement of the levels of steroid hormones. At the same time, this review also covers the most recent developments that have taken place in the recent past to cover the enhanced understanding of steroid hormone analysis.","PeriodicalId":91055,"journal":{"name":"Austin journal of analytical and pharmaceutical chemistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48232234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-21DOI: 10.26420/austinjanalpharmchem.2022.1149
Salem M, Ayyad R, S. H, El-Helby Ae, M. A, Mahmoud K
Arylidene derivatives were synthesized from phenyl hydrazine and series of different aldehydes. Arylidene compounds are important classes of N-containing fused heterocycles widely used as key building blocks for pharmaceutical agents due to a wide range of biological activities that include anti-inflammatory and antitumor properties [1]. Arylidene derivatives were furnished by the fusion of benzene ring with different aldehydes via hydrazine moiety in the presence of glacial acetic acid. These derivatives were characterized by TLC, melting points, Infrared Red, Nuclear Magnetic Resonance and Mass Spectroscopy. Finally, these synthetized derivatives were tested for analgesic activity using hot plate test method, anti-inflammatory activity using rat paw oedema and ulcer index test.
{"title":"Ulcer Index, Analgesics and Anti inflammatory Screening of Some Arylidene Compounds Derived from Phenyl Hydrazine and Aromatic Aldehydes","authors":"Salem M, Ayyad R, S. H, El-Helby Ae, M. A, Mahmoud K","doi":"10.26420/austinjanalpharmchem.2022.1149","DOIUrl":"https://doi.org/10.26420/austinjanalpharmchem.2022.1149","url":null,"abstract":"Arylidene derivatives were synthesized from phenyl hydrazine and series of different aldehydes. Arylidene compounds are important classes of N-containing fused heterocycles widely used as key building blocks for pharmaceutical agents due to a wide range of biological activities that include anti-inflammatory and antitumor properties [1]. Arylidene derivatives were furnished by the fusion of benzene ring with different aldehydes via hydrazine moiety in the presence of glacial acetic acid. These derivatives were characterized by TLC, melting points, Infrared Red, Nuclear Magnetic Resonance and Mass Spectroscopy. Finally, these synthetized derivatives were tested for analgesic activity using hot plate test method, anti-inflammatory activity using rat paw oedema and ulcer index test.","PeriodicalId":91055,"journal":{"name":"Austin journal of analytical and pharmaceutical chemistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45023675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-11DOI: 10.26420/austinjanalpharmchem.2022.1148
D. M
The purpose of this study was to verify the proximate analysis (DM percent, Ash percent, CP percent, EE percent, and CF percent) using AOAC approved methods at the Feed Quality Control Laboratory, DLS, Savar, Dhaka, and to reduce measurement uncertainty in each test in the laboratory. To verify the test procedures, a laboratory-made control sample (full-fat soybean), a certified control sample, and two Proficiency test feed samples (List A) were employed, together with various calibrate equipment (Table 1,2). This strategy was put to the test in terms of precision, accuracy, and consistency. Limits of Detection (LOD), Limits of Quantification (LOQ), Robustness, and Ruggedness, on the other hand, are irrelevant in these verification and uncertainty assessment techniques. Tests were carried out over a period of several months to verify this process and to assess the uncertainty more precisely. The Recovery percent was determined for the accuracy test, the Nordtest technique (Single Laboratory Verification Technique), and FAO. 2011 guidelines were used to assess the precision of the methods’ long-term repeatability. The recovery% of the determination of DM%, Ash%, CF%, EE%, and CP% of the Laboratory manufactured Reference sample, FAPAS Proficiency test sample-1, and Sample 2 (Tables 3-7) were within 98-102%, while the precision was determined by long-term repeatability (SRw) was 0.87%, 0.13%, 0.29%, 0.25%, and 0.35%, respectively, which met the criterion (Table 2) of FAO guideline. The measurement uncertainty was computed using the Nordtest method (Single Laboratory Verification Technique) and FAO. 2011 criteria, based on the longterm repeatability of the proximal components in the acknowledged laboratory. According to the guideline, the Expanded Measurement Uncertainty for DM percent, Ash percent, CF percent, EE percent, and CP percent was 0.99, 0.89, 1.02, and 1.82 percent, respectively, which was quite satisfactory. So, in the respective laboratory-Feed Quality Control Laboratory, Department of Livestock Services, Savar, Dhaka, Bangladesh-this verification for proximate components analysis of the animal feed is validated.
{"title":"Method Verification and Measurement of Uncertainty Estimation for the Proximate Analysis in Animal Feed-Single Laboratory Verification Protocol (Nordtest Approach)","authors":"D. M","doi":"10.26420/austinjanalpharmchem.2022.1148","DOIUrl":"https://doi.org/10.26420/austinjanalpharmchem.2022.1148","url":null,"abstract":"The purpose of this study was to verify the proximate analysis (DM percent, Ash percent, CP percent, EE percent, and CF percent) using AOAC approved methods at the Feed Quality Control Laboratory, DLS, Savar, Dhaka, and to reduce measurement uncertainty in each test in the laboratory. To verify the test procedures, a laboratory-made control sample (full-fat soybean), a certified control sample, and two Proficiency test feed samples (List A) were employed, together with various calibrate equipment (Table 1,2). This strategy was put to the test in terms of precision, accuracy, and consistency. Limits of Detection (LOD), Limits of Quantification (LOQ), Robustness, and Ruggedness, on the other hand, are irrelevant in these verification and uncertainty assessment techniques. Tests were carried out over a period of several months to verify this process and to assess the uncertainty more precisely. The Recovery percent was determined for the accuracy test, the Nordtest technique (Single Laboratory Verification Technique), and FAO. 2011 guidelines were used to assess the precision of the methods’ long-term repeatability. The recovery% of the determination of DM%, Ash%, CF%, EE%, and CP% of the Laboratory manufactured Reference sample, FAPAS Proficiency test sample-1, and Sample 2 (Tables 3-7) were within 98-102%, while the precision was determined by long-term repeatability (SRw) was 0.87%, 0.13%, 0.29%, 0.25%, and 0.35%, respectively, which met the criterion (Table 2) of FAO guideline. The measurement uncertainty was computed using the Nordtest method (Single Laboratory Verification Technique) and FAO. 2011 criteria, based on the longterm repeatability of the proximal components in the acknowledged laboratory. According to the guideline, the Expanded Measurement Uncertainty for DM percent, Ash percent, CF percent, EE percent, and CP percent was 0.99, 0.89, 1.02, and 1.82 percent, respectively, which was quite satisfactory. So, in the respective laboratory-Feed Quality Control Laboratory, Department of Livestock Services, Savar, Dhaka, Bangladesh-this verification for proximate components analysis of the animal feed is validated.","PeriodicalId":91055,"journal":{"name":"Austin journal of analytical and pharmaceutical chemistry","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78312863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-12DOI: 10.26420/austinjanalpharmchem.2022.1147
Hossain Mm, Hanna Asma, Kamal Mm, Hossain Ma, Quraishi Sb
Toxic metal mercury (Hg) in poultry feed might affect seriously on the environment and human being. So continual or gradual analyses of toxic metal contents in poultry feedstuff are of prime significance for assessing the feed quality and consumer safety. In this study, a method named Mercury Vapor Unit Atomic Absorption Spectrometry (MVU-AAS) was used to analyze the content of Hg in poultry feed in compliance with the Council Directive 333/2007/EC, Commission Decision 657/2002/EC. The metal Hg was analyzed from the feed sample inthe laboratory by MVU-AAS (Model: AA-7000 Shimadzu, Japan) and the absorbance was read at 253.65 nm wavelength, The method was confirmed and validated by complying with the international guidelines and assessing a number of criteria say system suitability, precision studies, linearity check, uncertainty measurement and recovery % etc.. The results revealed that the coefficient of variation (CV%) for system suitability and precision was <10% for the metal (Hg) detected in this study. The linearity of the calibration curves was excellent (r2>0.998) at various concentrated levels. The limits of detection (LoD) value in feed was 0.007 mg/Kg and the limit of quantification (LoQ) value in feed sample was found 0.023 mg/Kg. The values of instrumental detection limit (IDL) and instrument quantification limit (IQL) were 0.045(μg/l), and 0.15(μg/l), respectively. The recovery (%) found for the metal was 92.29 %. The overall CV% of repeatability and reproducibility ranged from 3.27 % to 5.92 %. The value ranges from 12 to 16 was observed for the measurement uncertainty (%) of Hg in this study. The proposed validation criteria indicate that it is a reliable method that can be used easily for the routine analysis of toxic metal (Hg) content in poultry feed sample.
{"title":"Appraisal and Validation of a Method (MVU-AAS) Used for the Detection of a Toxic Metal Mercury (Hg) in Poultry Feed Available in Bangladesh","authors":"Hossain Mm, Hanna Asma, Kamal Mm, Hossain Ma, Quraishi Sb","doi":"10.26420/austinjanalpharmchem.2022.1147","DOIUrl":"https://doi.org/10.26420/austinjanalpharmchem.2022.1147","url":null,"abstract":"Toxic metal mercury (Hg) in poultry feed might affect seriously on the environment and human being. So continual or gradual analyses of toxic metal contents in poultry feedstuff are of prime significance for assessing the feed quality and consumer safety. In this study, a method named Mercury Vapor Unit Atomic Absorption Spectrometry (MVU-AAS) was used to analyze the content of Hg in poultry feed in compliance with the Council Directive 333/2007/EC, Commission Decision 657/2002/EC. The metal Hg was analyzed from the feed sample inthe laboratory by MVU-AAS (Model: AA-7000 Shimadzu, Japan) and the absorbance was read at 253.65 nm wavelength, The method was confirmed and validated by complying with the international guidelines and assessing a number of criteria say system suitability, precision studies, linearity check, uncertainty measurement and recovery % etc.. The results revealed that the coefficient of variation (CV%) for system suitability and precision was <10% for the metal (Hg) detected in this study. The linearity of the calibration curves was excellent (r2>0.998) at various concentrated levels. The limits of detection (LoD) value in feed was 0.007 mg/Kg and the limit of quantification (LoQ) value in feed sample was found 0.023 mg/Kg. The values of instrumental detection limit (IDL) and instrument quantification limit (IQL) were 0.045(μg/l), and 0.15(μg/l), respectively. The recovery (%) found for the metal was 92.29 %. The overall CV% of repeatability and reproducibility ranged from 3.27 % to 5.92 %. The value ranges from 12 to 16 was observed for the measurement uncertainty (%) of Hg in this study. The proposed validation criteria indicate that it is a reliable method that can be used easily for the routine analysis of toxic metal (Hg) content in poultry feed sample.","PeriodicalId":91055,"journal":{"name":"Austin journal of analytical and pharmaceutical chemistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48797021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-08DOI: 10.26420/austinjanalpharmchem.2022.1146
Hosain Mz, Islam Sms, Kamal Mm
Chloramphenicol (CAP) is a broad-spectrum antibiotic widely applied in veterinary practices. Continual use of CAP in livestock production may lead to antibiotic resistance and health-related hazards. In this study, a rapid and highly sensitive analytical method based on ultra-performance liquid chromatographytandem mass spectrometry (UPLC–MS/MS) was developed and validated to detect and quantify there is due of CAP in poultry meat regarding the safety of humans and animals. Total 80 samples of poultry meat were collected from different poultry farms, and poultry meat sellers in three upazilas namely- Sadar, Mirzapur and Ghatail of Tangail district in the Dhaka division. The test analysis was performed using matrix-matched liquid chromatography-mass spectrophotometry. The chromatographic separation of the CA Presidue was carried out at 40°C temperature on a reverse-phase C18 column using a binary gradient pump, and quantification was performed by LC-MS/MS in electrospray mode. Mobile phase constituents were solvent (a) deionized water, and (b) acetonitrile. The flow rate was 0.35 mL/min and the total run time was 5 min. The method was validated in terms of selectivity, linearity, recovery, and precision following the 2021/808/EC guidelines and acceptance criteria were met in all the cases. The relative standard deviation (RSD) for precision was <11%. The linearity of the calibration curves was excellent (R2 >0.999) at concentrations of 0.25, 0.50, 0.75, 1.0, 2.0, and 5.0 μg/kg for matrix-matched CAP standard, and the range of linearity of this method was 0.0-5.0 μg/kg with R2 value greater than 0.99. The decision limit (CC-a), and detection capability (CCβ) were 0.29 μg/ kg, and 0.31 μg/kg respectively, and the recovery percentages ranged from 94 to 100 %. In this study, the levels of CAP residue in tested poultry meat samples were found below the detection limit. The overall parameters of the proposed method met the validation criteria, and the method proved to be suitable for CAP residues determination in poultry meat samples. Thus, this method could be a precise and highly desirable analytical procedure for rapid and routine analysis of CA Presidues in poultry meat, and obtained tested results in this study could be an authentic data to ensure the chloramphenicol free safety poultry meat for human consumption.
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