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Yacon juice as culture and cryoprotectant medium for Latilactobacillus sakei and Staphylococcus vitulinus autochthonous strains 雪糕汁作为酿酒乳酸菌和白葡萄球菌本土菌株的培养和冷冻保护剂
Q3 Agricultural and Biological Sciences Pub Date : 2023-02-06 DOI: 10.1590/1981-6723.11922
Noelia Palavecino Prpich, Ernesto Sanabria, M. F. Gliemmo, M. Cayré, M. Castro
Abstract Yacon is mainly constituted of water and carbohydrates [single sugars and fructooligosaccharides (FOS)], thus being an excellent alternative for the growth and preservation of bacterial culture. Latilactobacillus sakei ACU-2 and Staphylococcus vitulinus ACU-10 comprised the autochthonous starter culture SAS-1 designed for the manufacture of dry sausages. This study evaluated the use of yacon juice as a potential growth medium and cryoprotectant for these bacteria. The growth medium was prepared with yacon juice supplemented with peptone and dipotassium phosphate. After growing, cells were resuspended in yacon juice (5, 10 and 25 mL/100 mL) and lyophilized. Viable cells were count before, immediately after lyophilization, and along 6 months of refrigerated storage. Both bacteria grew in every yacon concentration tested; however, juice concentration affected their growth. Latilactobacillus sakei grew at μ = 0.256 ± 0.01 giving the highest bacterial density at 10 mL/100 mL (Log DOmax 0.33 ± 0.01). While 5 mL/100 mL yacon juice provided the best conditions for S. vitulinus growth (μ = 0.215 ± 0.016; Log DOmax 0.32 ± 0.01). After lyophilization, the survival rate was 91.1% for L. sakei and 65.8% for S. vitulinus. Throughout storage, high cell counts suggested good stability of both bacteria. Results revealed that yacon juice comprises a nutritive substrate for the growth and cryopreservation of tested strains from the genus Latilactobacillus and Staphylococcus.
摘要雪莲果主要由水和碳水化合物(单糖和低聚果糖)组成,是细菌培养物生长和保存的优良替代品。酒井乳酸杆菌ACU-2和白葡萄球菌ACU-10组成了为生产干香肠而设计的本土发酵剂SAS-1。本研究评估了雪糕汁作为这些细菌的潜在生长培养基和冷冻保护剂的使用。以雪莲果汁为培养基,添加蛋白胨和磷酸二钾。细胞生长后,用雪糕汁(5、10和25 mL/100 mL)重悬并冻干。在冻干前、冻干后和冷藏6个月时分别对活细胞进行计数。两种细菌在每一种测试浓度的雪莲中都有生长;但果汁浓度对其生长有影响。酒井乳酸杆菌在μ = 0.256±0.01时生长,10 mL/100 mL时细菌密度最高(Log DOmax为0.33±0.01)。而5 mL/100 mL雪莲汁的最佳生长条件为(μ = 0.215±0.016;Log DOmax 0.32±0.01)。经冻干处理后,sakei和S. vitulinus的存活率分别为91.1%和65.8%。在整个储存过程中,高细胞计数表明这两种细菌都具有良好的稳定性。结果表明,雪莲果汁含有供乳酸杆菌属和葡萄球菌属菌株生长和低温保存的营养基质。
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引用次数: 0
Determination of exogenous water in grape juice through the isotopic analysis of 18O/16O 18O/16O同位素分析法测定葡萄汁中外源水分
Q3 Agricultural and Biological Sciences Pub Date : 2023-01-27 DOI: 10.1590/1981-6723.17222
Mirella Mallmann Kercher, S. Leonardelli, Gilberto João Cargnel, R. Vanderlinde
Abstract The consumption of grape juice has been growing significantly, so its quality is becoming an issue of great importance, both for the consumer and for the industry. However, identifying adulteration in juice is a great challenge and requires a reliable analytical process. The isotope ratio (18O/16O) is an important tool to determine the addition of exogenous water in beverages, however, there is no official method for juice in Brazil. This study aimed to develop and validate a method for detecting exogenous water in grape juice through isotopic analysis of 18O/16O. The development and validation of the analytical method were performed using Isotope Ratio Mass Spectrometry (IRMS). The effect of temperature and evaporation of δ18O in experimental juices was evaluated, and reference values were found for juices based on the δ 18O of musts. The influence of the juice industrial production process on 18O values was verified, and commercial juices were evaluated in relation to the values of reference regarding the addition of water. The temperature and evaporation parameters did not influence the results of the 18O of the juice, as they presented differences lower than the method uncertainty. The heat exchanger system did not influence the proposed method. The reference values for juice can come from the musts, without affecting the interpretation of the final results. Of the thirty real juices analyzed, nine had exogenous water, three proved to be reconstituted juices and eighteen were considered to have no exogenous water. The method proposed and validated in this study presented values for the limit of detection (LOD) of 0.24‰, the limit of quantification (LOQ) of 0.97‰ and measurement uncertainty of 0.71‰, proving to be effective for the detection of exogenous water in grape juice, through of the analysis of the isotopic ratio of 18O/16O by IRMS.
摘要葡萄汁的消费量一直在显著增长,因此葡萄汁的质量对消费者和行业来说都是一个非常重要的问题。然而,识别果汁中的掺假是一个巨大的挑战,需要一个可靠的分析过程。同位素比率(18O/16O)是确定饮料中外源水添加的重要工具,然而,巴西没有官方的果汁方法。本研究旨在建立并验证一种通过18O/16O同位素分析来检测葡萄汁中外源水分的方法。采用同位素比质谱(IRMS)对分析方法进行了开发和验证。评价了温度和蒸发对实验果汁δ18O的影响,并以must的δ18O为基准得出了果汁的参考值。验证了果汁工业生产过程对18O值的影响,并评估了商业果汁在添加水方面的参考值。温度和蒸发参数的差异小于方法不确定度,对果汁18O的测定结果没有影响。换热器系统对所提出的方法没有影响。果汁的参考值可以来自must,而不影响对最终结果的解释。在分析的30种真实果汁中,9种含有外源水,3种被证明是重组果汁,18种被认为没有外源水。本研究提出并验证的方法检出限为0.24‰,定量限为0.97‰,测量不确定度为0.71‰,通过IRMS对18O/16O同位素比值的分析,证明该方法对葡萄汁中外源水的检测是有效的。
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引用次数: 0
The usefulness of ecotoxicological approaches to assess environmental impacts caused by oil spills 生态毒理学方法评估石油泄漏造成的环境影响的有效性
Q3 Agricultural and Biological Sciences Pub Date : 2023-01-27 DOI: 10.38152/bjtv6n1-005
D. Abessa
Accidents involving oil spills have a high potential to cause catastrophic environmental impacts. However, the Brazilian legislation and guidelines to plan and determine response actions in case of oil accidents are vague and generally do not include the assessment of toxic effects of oil on the exposed biota and ecosystems. Therefore, such actions are unable to adequately assess the environmental effects of oil in the short, medium and long terms. Ecotoxicological methods consist of cost-effective and robust alternatives to identify and quantify the effects of oil on the exposed ecosystems. Such approaches should be incorporated into response actions in episodes of oil spills in aquatic environments.
涉及石油泄漏的事故极有可能造成灾难性的环境影响。然而,巴西在石油事故中规划和确定应对行动的立法和准则是含糊不清的,通常不包括评估石油对暴露的生物群和生态系统的毒性影响。因此,此类行动无法充分评估石油在短期、中期和长期的环境影响。生态毒理学方法包括具有成本效益和可靠的替代方法,以确定和量化石油对暴露的生态系统的影响。这些办法应纳入在水生环境中发生溢油事件时采取的应对行动。
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引用次数: 0
Aplicação do FMEA na prevenção de acidentes ocupacionais na construção civil FMEA在土木建筑职业事故预防中的应用
Q3 Agricultural and Biological Sciences Pub Date : 2023-01-24 DOI: 10.38152/bjtv6n1-004
Lucio Garcia Veraldo Junior, Matheus Marcelino Lescura, Henrique César Sampaio, Paulo França Barbosa Neto
Esta pesquisa trata da aplicação da ferramenta FMEA na construção civil a fim de prevenir acidentes ocupacionais durante o trabalho. Diante das características das atividades, a construção civil é um dos setores mais problemáticos em relação à ocorrência de acidentes do trabalho. Assim, a implementação da segurança ocupacional é extremamente importante para prevenir acidentes, e a utilização da ferramenta FMEA para analisar riscos ocupacionais definindo medidas preventivas para os trabalhadores. O propósito desta pesquisa é por meio do uso da ferramenta FMEA analisar os possíveis riscos no trabalho da construção civil, a fim de estabelecer a criticidade entre eles e implementar um plano de ações preventivas de modo a reduzir o número de acidentes. Neste estudo pode-se concluir que, por meio da utilização da ferramenta FMEA, é possível elencar os riscos de forma quantitativa e definir prioridades para ações. Assim, permitiu identificar a redução de acidentes e o alcance da meta estabelecida, resultando em 1 ocorrência durante um período de 6 meses.
本研究探讨FMEA工具在建筑施工中的应用,以防止工作过程中的职业事故。鉴于活动的特点,建筑是与工作事故发生有关的问题最严重的部门之一。因此,职业安全的实施对预防事故非常重要,使用FMEA工具分析职业风险,为工人制定预防措施。本研究的目的是利用FMEA工具分析建筑施工中可能存在的风险,以建立它们之间的临界性,并实施预防行动计划,以减少事故的数量。在本研究中,我们可以得出结论,通过使用FMEA工具,可以定量地列出风险并确定行动的优先次序。因此,它确定了事故的减少和既定目标的实现,导致在6个月内发生1起事故。
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引用次数: 0
Microbiological evaluation of feet-cutter disc for broiler with a self-washing system using heated and non-heated water 用热水和非热水自洗系统的肉鸡切脚盘的微生物学评价
Q3 Agricultural and Biological Sciences Pub Date : 2023-01-23 DOI: 10.1590/1981-6723.04622
Ricardo Cavani, M. Rubio, L. Alves, Khauston Augusto Pereira Alves, M. Cardozo
The increased worldwide demand for chicken meat requires constant automation of operating systems and the proper implementation of self-control programs by slaughterhouses. Thus, this study aimed to evaluate the efficacy of a self-washing system using heated water on reducing the load of public health concern bacteria on a broiler feet-cutter disc. In a three-month period, 84 samples (n = 42 per group) were collected for microbiological analysis from the feet-cutter disc with an operational self-washing process with Non-Heated Water (NHW) and Heated Water (HW). The logarithmic counts of Mesophilic Aerobics, Sulphite-Reducing Clostridia (SRC), Escherichia coli and Staphylococcus aureus in the NHW group were 1.82, 0.56, 0.31 and 0.14 Log 10 CFU/cm 2 , respectively, whereas in the HW group were 1.17, 0.04, 0.15 and 0.08 Log 10 CFU/cm 2 . Salmonella spp. was absent in all evaluated samples in both groups. The heated-water system reduced bacterial count, but statistical differences were solely observed for Mesophilic Aerobics and SRC. Nevertheless, its usage could be an additive in the operational procedure to avoid microbiological violations of food safety, in addition to being easy-to-implement and affordable for the industry.
世界范围内对鸡肉需求的增加要求操作系统不断自动化,并要求屠宰场适当实施自我控制程序。因此,本研究旨在评估使用热水的自洗系统对减少肉鸡切脚盘上公共卫生问题细菌负荷的功效。在三个月的时间里,采用非热水(NHW)和热水(HW)自洗工艺,从切脚盘上收集84个样本(每组n = 42个)进行微生物分析。NHW组嗜酸性好氧梭菌、亚硫酸盐还原梭菌、大肠杆菌和金黄色葡萄球菌的对数分别为1.82、0.56、0.31和0.14 Log 10 CFU/cm 2,而HW组的对数分别为1.17、0.04、0.15和0.08 Log 10 CFU/cm 2。两组样品均未发现沙门氏菌。热水系统降低了细菌数量,但统计学差异仅在嗜温好氧菌和SRC中观察到。尽管如此,它的使用可以作为操作程序中的添加剂,以避免微生物违反食品安全,除了易于实施和负担得起的行业。
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引用次数: 0
Adição de cinzas de casca de ovo e concha marinhas para melhorar as propriedades físicas e mecânicas do concreto 添加蛋壳灰和贝壳灰,改善混凝土的物理力学性能
Q3 Agricultural and Biological Sciences Pub Date : 2023-01-13 DOI: 10.38152/bjtv6n1-003
Abigail Kenia Hurtado Cueva, Mayli Pariona Auccatoma, Luis Jimmy Clemente Condori, Leopoldo Choque Flores
Nossa pesquisa visa modificar as propriedades físicas e mecânicas do concreto pela adição de cinzas de casca de ovo e concha marinhas. Esta investigação ocorre em decorrência da implantação de novas adições de materiais alternativos onde há a necessidade de se investigar a adição de cinzas de casca de ovo e concha marinhas e como isso pode influenciar nas propriedades físicas e mecânicas do concreto. A investigação teve um delineamento experimental, onde foram realizados 36 tubos de ensaio de resistência à compressão e 36 tubos de ensaio de tração e 24 ensaios físicos, onde foram adicionados CCH e CCA nas proporções de 2% CCH e 1% CCA; 4% CCH e 1,5% CCA e 6% CCH e 2,5% CCA que foram avaliados aos 7, 14 e 28 dias.Portanto, concluiu-se que a adição de CCH e CCA em (4% e 1,5%) e (6% e 2,5%) modificam as propriedades negativamente, enquanto as porcentagens de menor adição de cinzas (2% e 1%) modificam positivamente melhorando propriedades físicas e mecânicas do concreto.
本研究旨在通过添加蛋壳灰和贝壳灰来改善混凝土的物理力学性能。这项研究是由于在有必要研究蛋壳和贝壳灰的添加以及它如何影响混凝土的物理和机械性能的情况下,实施了新的替代材料添加。本研究采用实验设计,共进行了36个抗压强度试管和36个拉伸试管,并进行了24项物理试验,分别以2% CCH和1% CCA的比例加入CCH和CCA;分别于第7、14和28 d测定4% hcc、1.5% hcc、6% hcc和2.5% hcc。因此,我们得出结论,在(4%和1.5%)和(6%和2.5%)中添加CCH和CCA对混凝土的性能有负面影响,而添加少量灰分(2%和1%)对混凝土的物理力学性能有积极影响。
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引用次数: 0
Adição de resíduos de café e cana-de-açúcar na resistência à compressão e à tração do concreto estrutural 添加咖啡渣和甘蔗对结构混凝土抗压和抗拉强度的影响
Q3 Agricultural and Biological Sciences Pub Date : 2023-01-12 DOI: 10.38152/bjtv6n1-002
Ana Iris Castro Mendoza, Fiorela Yselina Perez Pereda, Luis Jimmy Clemente Condori, Leopoldo Choque Flores
A pesquisa sobre a adição de resíduos de café e cana-de-açúcar na resistência à compressão e à tração do concreto estrutural teve como objetivo principal melhorar a resistência à compressão e à tração do concreto estrutural através da adição de resíduos da casca de café e cana-de-açúcar na proporção de GE 1 (2% CCC + 0.25% FBCA), GE 2 (4% CCC + 0.25% FBCA), GE 3 (2% CCC + 0.50%) e GE 4 (4% CCC + 0.50% FBCA) em substituição ao agregado miúdo, portanto, a investigação se justifica para melhorar as propriedades do concreto e sua contribuição para o ambiente. A metodologia usado um tipo e delineamento de pesquisa aplicada - experimental, que foi de amostragem não probabilística em que houve um padrão e amostra experimental de acordo com os critérios relevantes do pesquisador com Nesta investigação, o foram realizados os respectivos testes do concreto com base em 3 tubos de ensaio por dose de incorporação de cinza de casca de café e fibras de bagaço de cana para comodidade do pesquisador que incluiu vários fatores, posteriormente foram realizados os testes em laboratório e por fim foram analisados pelo Minitab ferramentas estatísticas.
研究添加咖啡和甘蔗废料在压缩和拉伸强度的混凝土结构主要是为了改善混凝土的压缩和拉伸强度增加结构性浪费的咖啡和甘蔗在由通用电气1 (CCC + 0 2%比例FBCA),通用电气CCC + 0分别FBCA 2(4%),通用电气3 (CCC + 0除了2%)和通用电气(GE) 4 (CCC + 0 4%除了FBCA)代替孩子,所以总为了改善混凝土的性能及其对环境的贡献,这项研究是合理的。的使用方法和应用研究设计的一个人—概率抽样实验,这是有一个标准和样品实验根据相关标准的研究和调查,具体的执行他们的测试基于三灰剂量的挑战是试管的咖啡和甘蔗蔗渣纤维品质的研究包括很多因素,随后在实验室进行测试,最后用Minitab统计工具进行分析。
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引用次数: 0
Comparação da fibra acrílica e da fibra de borracha nas propriedades físicas e mecânicas do concreto f'c= 280 kg/cm2 丙烯酸纤维和橡胶纤维对混凝土f'c= 280kg /cm2物理力学性能的比较
Q3 Agricultural and Biological Sciences Pub Date : 2023-01-12 DOI: 10.38152/bjtv6n1-001
Aldair Enrique Sandoval Tamariz, Jeremi Jheferson Solano Vega, Luis Jimmy Clemente Condori, Leopoldo Choque Flores
O objetivo deste artigo é mostrar a diferença de desempenho nas propriedades físicas e mecânicas entre a fibra de borracha e a fibra acrílica incorporada ao concreto f'c=280 kg/cm2. A pesquisa é do tipo aplicada, com abordagem quantitativa e em nível experimental. Foram confeccionados 126 espécimes com as proporções de FA (0,3%, 0,6%, 0,9%) e FC (3%, 5%, 7%) avaliados aos 7 e 28 dias. Nosso propósito é encontrar novos materiais ecológicos que possam produzir concretos sustentáveis que atuem com mais eficiência na construção civil. Os resultados obtidos no artigo mostram que a adição de 0,3% de FA aumenta a resistência à compressão e tração em 13,14% e 9,81%, porém, nos ensaios no estado fresco, qual é o peso unitário e o recalque, uma redução.
本文的目的是展示橡胶纤维和丙烯酸纤维在混凝土f'c=280 kg/cm2的物理力学性能上的差异。本研究为应用研究,采用定量研究和实验研究相结合的方法。采用FA(0.3%, 0.6%, 0.9%)和FC(3%, 5%, 7%)的比例制备126个标本,分别于第7天和第28天进行评估。我们的目标是寻找新的生态材料,可以生产可持续的混凝土,在建筑中更有效地工作。结果表明,添加0.3%的FA可使其抗压强度和抗拉强度分别提高13.14%和9.81%,但在新鲜状态下的试验中,单位重量和沉降降低。
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引用次数: 0
Knowledge and good food handling practices for products of animal origin in the home setting 家庭环境中动物源性产品的知识和良好的食品处理规范
Q3 Agricultural and Biological Sciences Pub Date : 2023-01-01 DOI: 10.1590/1981-6723.05523
Eduarda de Cássia Lima Brugeff, Fernando de Almeida Borges, Daniele Bier
Abstract Good food handling practices should be adopted in the home setting, as inadequate practices could lead to the outbreak of foodborne illnesses. The aim of the present study was to investigate knowledge and good food handling practices for products of animal origin by consumers in the home setting and test associations with the socioeconomic characteristics of the respondents. A questionnaire was answered by a non-probabilistic sample. The questionnaire was available online on free access digital platforms for seven days and was used to collect information on the gender, age, income, and schooling of the respondents as well as safe food handling practices in the home setting. Data analysis involved descriptive statistics and the chi-square test to compare the frequencies of the answers per category. A total of 666 responses were obtained. Most respondents were women, had a university/college education, were between 30 and 59 years of age, and had an income of more than four times the monthly minimum wage. The majority of respondents reported positive practices and attitudes regarding food handling in the home setting; 99.5% washed their hands prior to handling food, 89% performed the cooking of animal products and 85.6% washed the cutting surface after cutting each food. Associations were found between some attitudes reported by consumers and the categories of gender, age, schooling, and income (p ≤ 0.05). Women had more good food handling practices compared to men. The results suggest that most respondents practiced good food handling practices. Safe practices were related to the level of schooling, monthly income and age of the participants.
良好的食品处理规范应在家庭环境中采用,因为不适当的规范可能导致食源性疾病的爆发。本研究的目的是调查家庭环境中消费者对动物源性产品的知识和良好食品处理规范,并测试与受访者社会经济特征的关联。问卷是用非概率样本来回答的。该问卷可在免费访问的数字平台上在线获取七天,用于收集有关受访者的性别、年龄、收入和学校教育以及家庭环境中安全食品处理做法的信息。数据分析涉及描述性统计和卡方检验,以比较每个类别的答案频率。共收到666份回复。大多数受访者是女性,受过大学/学院教育,年龄在30至59岁之间,收入是每月最低工资的四倍以上。大多数答复者报告了在家庭环境中处理食物的积极做法和态度;99.5%的人在处理食物前洗手,89%的人烹煮动物产品,85.6%的人在切完每一种食物后清洗切面。消费者报告的一些态度与性别、年龄、教育程度和收入类别之间存在关联(p≤0.05)。与男性相比,女性有更多好的食物处理方法。结果表明,大多数受访者都有良好的食品处理习惯。安全实践与参与者的教育程度、月收入和年龄有关。
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引用次数: 0
Physicochemical properties of extrudate-based flakes from whole banana flour and rice flour blends 由全香蕉粉和米粉混合制成的挤出基薄片的物理化学性质
Q3 Agricultural and Biological Sciences Pub Date : 2023-01-01 DOI: 10.1590/1981-6723.02923
Ronel Joel Bazán-Colque, Fiorella Ivette Ruiz-Barreto, Jhony Willian Vargas-Solórzano, Arturo Meléndez-Arévalo, José Luis Ramírez Ascheri
This study aimed to understand how adding whole-banana flour (WBF) affected the physicochemical properties of extrudates intended for the development of breakfast flakes. The parameters: WBF addition (50% to 70%, dry basis), feed moisture (27% to 33%, wet basis), and barrel temperature in the last zone of the extruder (80 ºC to 100 °C) were varied according to a 23 factorial design with augmented center points. The addition of WBF linearly affected all the physical and chemical properties evaluated and showed a synergistic effect with barrel temperature for changes in hardness and carbohydrate content. WBF additions above 64.9%, adjusted to feed moisture between 29.6% to 32.6%, and thermomechanically cooked at barrel temperatures above 87 °C, consumed 216 to 257 kJ/kg of mechanical energy, which produced flakes with a hardness between 160 and 180 N. This work could contribute to the incorporation of WBF for developing more nutritious breakfast cereals, with a fiber content greater than 7.2 g/100 g.
本研究旨在了解添加全香蕉面粉(WBF)如何影响用于早餐片开发的挤出物的物理化学性质。参数:WBF添加量(50%至70%,干基),饲料水分(27%至33%,湿基),以及挤出机最后区域的筒体温度(80ºC至100°C)根据23因子设计增加中心点。WBF的添加对所有理化性质均有线性影响,并与桶温对硬度和碳水化合物含量的变化有协同效应。WBF添加量在64.9%以上,调节进料水分在29.6% ~ 32.6%之间,在87℃以上的桶温下进行热机械蒸煮,消耗216 ~ 257 kJ/kg的机械能,得到硬度在160 ~ 180 n之间的薄片。该研究有助于WBF的加入,开发出纤维含量大于7.2 g/100 g的营养丰富的早餐谷物。
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引用次数: 0
期刊
Brazilian Journal of Food Technology
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