Pub Date : 2023-06-19DOI: 10.30744/brjac.2179-3425.ar-122-2022
Lana Pereira, Robson Brito, Kelly Dantas
The objective of this study was to determine and evaluate the bioaccessibility of Ca, Fe, Mg, and Mn in Apis mellifera and Melipona propolis samples. Flame atomic absorption spectrometry was used for the determination of total Ca, Fe, Mg, and Mn in digested propolis, and of the bioaccessible fractions. The levels of Ca, Fe, Mg, and Mn obtained after digestion ranged from 423.43 – 4368.42, 3.85 – 814.92, 139.02 – 1170.68, and 14.28 – 67.29 mg kg–1, respectively. The bioaccessible contents after simulated gastric digestion of Ca, Fe, Mg, and Mn ranged from 17.62 – 63.35, 0.12 – 1.64, 26.06 – 86.34, and 12.08 – 83.05%, respectively. In the simulation of the gastrointestinal tract, the concentrations ranged from 3.05 – 73.08% for Ca, 0.63 – 2.45% for Fe, 0.16 – 43.26% for Mg, and 1.47 – 8.45% for Mn. In general, the simulation showed higher bioaccessible contents after gastric, compared to gastrointestinal digestion.
{"title":"Bioaccessibility of Ca, Fe, Mg, and Mn in Brazilian Propolis","authors":"Lana Pereira, Robson Brito, Kelly Dantas","doi":"10.30744/brjac.2179-3425.ar-122-2022","DOIUrl":"https://doi.org/10.30744/brjac.2179-3425.ar-122-2022","url":null,"abstract":"The objective of this study was to determine and evaluate the bioaccessibility of Ca, Fe, Mg, and Mn in Apis mellifera and Melipona propolis samples. Flame atomic absorption spectrometry was used for the determination of total Ca, Fe, Mg, and Mn in digested propolis, and of the bioaccessible fractions. The levels of Ca, Fe, Mg, and Mn obtained after digestion ranged from 423.43 – 4368.42, 3.85 – 814.92, 139.02 – 1170.68, and 14.28 – 67.29 mg kg–1, respectively. The bioaccessible contents after simulated gastric digestion of Ca, Fe, Mg, and Mn ranged from 17.62 – 63.35, 0.12 – 1.64, 26.06 – 86.34, and 12.08 – 83.05%, respectively. In the simulation of the gastrointestinal tract, the concentrations ranged from 3.05 – 73.08% for Ca, 0.63 – 2.45% for Fe, 0.16 – 43.26% for Mg, and 1.47 – 8.45% for Mn. In general, the simulation showed higher bioaccessible contents after gastric, compared to gastrointestinal digestion.","PeriodicalId":9115,"journal":{"name":"Brazilian Journal of Analytical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48716704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-19DOI: 10.30744/brjac.2179-3425.ar-18-2023
Ma.Helen P. Seco, Eliana Larruscain, Jader Silveira, R. Picoloto, F. Duarte
In this work, a new method for Fe extraction and preconcentration from beer by using dispersive liquid-liquid microextraction (DLLME) and determination by flame atomic absorption spectrometry (F AAS) was developed. Although in DLLME the use of an extraction solvent is mandatory (at least in theory), which is combined with a disperser solvent, in this work only the extraction solvent was used. For Fe extraction by DLLME, the type and volume of extraction solvent, sample volume, type of complexing agent, sample pH, salting out effect, and the number of washing cycles after extraction were evaluated. Thus, after optimization, 10 mL of beer, 75 µL of 1,2-dichlorobenzene of extraction solvent, 500 µL of 0.5% (w/v) pyrrolidine ammonium dithiocarbamate (APDC) as complexing agent were used. It is important to mention that pH adjustment, salt addition or washing cycles were no required. For Fe determination after DLLME, an external calibration curve was performed (R2 > 0.99) by direct extracts (reference solutions through DLLME) introduction in the conventional nebulization system of F AAS instrument. The accuracy evaluation was performed by results comparison with those obtained after microwave-assisted digestion (MAD) and Fe determination by inductively coupled plasma optical emission spectrometry (ICP-OES). The Fe concentration in the studied samples ranged from 59.7 to 68.2 µg L-1 and the limit of quantification for DLLME/F AAS method was 9.0 µg L-1. Finally, the proposed method DLLME/F AAS was applied for Fe determination in several beer samples showing simplicity, low operational costs, suitable accuracy (104 to 119%) and precision (better than 13%), opening the possibility for its use in routine analysis for Fe determination in beer.
{"title":"A Simple Approach for Fe Determination in Beer by using Dispersive Liquid-Liquid Microextraction and Flame Atomic Absorption Spectrometry","authors":"Ma.Helen P. Seco, Eliana Larruscain, Jader Silveira, R. Picoloto, F. Duarte","doi":"10.30744/brjac.2179-3425.ar-18-2023","DOIUrl":"https://doi.org/10.30744/brjac.2179-3425.ar-18-2023","url":null,"abstract":"In this work, a new method for Fe extraction and preconcentration from beer by using dispersive liquid-liquid microextraction (DLLME) and determination by flame atomic absorption spectrometry (F AAS) was developed. Although in DLLME the use of an extraction solvent is mandatory (at least in theory), which is combined with a disperser solvent, in this work only the extraction solvent was used. For Fe extraction by DLLME, the type and volume of extraction solvent, sample volume, type of complexing agent, sample pH, salting out effect, and the number of washing cycles after extraction were evaluated. Thus, after optimization, 10 mL of beer, 75 µL of 1,2-dichlorobenzene of extraction solvent, 500 µL of 0.5% (w/v) pyrrolidine ammonium dithiocarbamate (APDC) as complexing agent were used. It is important to mention that pH adjustment, salt addition or washing cycles were no required. For Fe determination after DLLME, an external calibration curve was performed (R2 > 0.99) by direct extracts (reference solutions through DLLME) introduction in the conventional nebulization system of F AAS instrument. The accuracy evaluation was performed by results comparison with those obtained after microwave-assisted digestion (MAD) and Fe determination by inductively coupled plasma optical emission spectrometry (ICP-OES). The Fe concentration in the studied samples ranged from 59.7 to 68.2 µg L-1 and the limit of quantification for DLLME/F AAS method was 9.0 µg L-1. Finally, the proposed method DLLME/F AAS was applied for Fe determination in several beer samples showing simplicity, low operational costs, suitable accuracy (104 to 119%) and precision (better than 13%), opening the possibility for its use in routine analysis for Fe determination in beer.","PeriodicalId":9115,"journal":{"name":"Brazilian Journal of Analytical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41806955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-02DOI: 10.30744/brjac.2179-3425.ar-21-2023
L. Falchi, M. Pistón, Gabriela Casarotto, S. Carro, C. Cajarville, Fiorella Iaquinta
A simple and fast method for the simultaneous determination of chloride and lactic acid in artisanal Danbo cheese was developed and validated. The proposed methodology combined a sample treatment using only ultrapure water as an extracting agent and ion chromatography with suppressed conductivity for the detection of the analytes. One gram of sample exactly weighted is added to 40.0 mL of ultrapure water at 55 ºC. The suspension was stirred for 5 minutes and after centrifugation the supernatant obtained is ready to be used for the determination of both analytes. The performance of this method was evaluated by comparing the results obtained with those acquired after the analysis of the cheese samples using the AOAC reference methods using a t-student test of mean concentration values. At a 95% significance level, the means obtained by the different methods were comparable. Analytical precision expressed as relative standard deviation (RSD %) was less than 6. Artisanal cheese samples were analyzed (n=12) using the validated methodology, obtaining chloride levels between 0.6 and 1.1% w w-1 expressed in sodium chloride and levels of lactic acid between 1.1 and 2.1% w w-1. Additionally, a greenness analysis was presented, using three different metric tools, concluding that the developed method is significantly in better agreement with the principles of Green Analytical Chemistry than the official methods.
{"title":"Development of a Green Methodology for the Determination of Artisanal Danbo Cheese Quality Parameters","authors":"L. Falchi, M. Pistón, Gabriela Casarotto, S. Carro, C. Cajarville, Fiorella Iaquinta","doi":"10.30744/brjac.2179-3425.ar-21-2023","DOIUrl":"https://doi.org/10.30744/brjac.2179-3425.ar-21-2023","url":null,"abstract":"A simple and fast method for the simultaneous determination of chloride and lactic acid in artisanal Danbo cheese was developed and validated. The proposed methodology combined a sample treatment using only ultrapure water as an extracting agent and ion chromatography with suppressed conductivity for the detection of the analytes. One gram of sample exactly weighted is added to 40.0 mL of ultrapure water at 55 ºC. The suspension was stirred for 5 minutes and after centrifugation the supernatant obtained is ready to be used for the determination of both analytes. The performance of this method was evaluated by comparing the results obtained with those acquired after the analysis of the cheese samples using the AOAC reference methods using a t-student test of mean concentration values. At a 95% significance level, the means obtained by the different methods were comparable. Analytical precision expressed as relative standard deviation (RSD %) was less than 6. Artisanal cheese samples were analyzed (n=12) using the validated methodology, obtaining chloride levels between 0.6 and 1.1% w w-1 expressed in sodium chloride and levels of lactic acid between 1.1 and 2.1% w w-1. Additionally, a greenness analysis was presented, using three different metric tools, concluding that the developed method is significantly in better agreement with the principles of Green Analytical Chemistry than the official methods.","PeriodicalId":9115,"journal":{"name":"Brazilian Journal of Analytical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45596583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-02DOI: 10.30744/brjac.2179-3425.ar-4-2023
Rayane Costa, J. V. Santiago, E. Ferreira, A. Virgilio, J. G. Gomes Neto
The closed-vessel conductively heated digestion system (CHDS) was evaluated to digest dry dog foods for further determination of K, Na, Cu, Fe, Mn, and Zn by high-resolution continuum source flame atomic absorption spectrometry (HR-CS FAAS). The CHDS method was optimized using a fractional factorial design with five variables (HNO3 concentration, H2O2 volume, temperature, holding time, and pre-digestion time) at two levels. The accuracy of the CHDS procedure was checked by the analysis of reference materials from the National Institute of Standards and Technology (NIST SRM 1577b Bovine Liver and 2976 Mussel Tissue) and Brazilian Agricultural Research Corporation (Embrapa MR-E1002A Fish Food). Also, the digestion efficiencies were calculated from residual carbon contents (RCCs). The RCC and blank values in the CHDS digested samples were consistently low, which is suitable for determinations using ICP OES and ICP-MS techniques. For comparison, all samples were also digested by microwave-assisted digestion in closed vessels (MW-AD). Results for Na, K, Cu, Fe, Mn, and Zn determined in sample digests obtained by CHDS were not statistically different at a 95% confidence level from those observed for MW-AD. Limits of quantification (LOQ) calculated from digests in CHDS and MW-AD were comparable, and the values provided adequate limits for elemental determinations in dog foods. Data from mineral composition and moisture were employed in a clustering analysis (HCA) and the discrimination of the samples among different manufacturers and food for dogs at different life stages was possible.
{"title":"Closed-Vessel Conductively Heated Digestion of Dry Dog Food for Spectrometric Determination of Essential Nutrients","authors":"Rayane Costa, J. V. Santiago, E. Ferreira, A. Virgilio, J. G. Gomes Neto","doi":"10.30744/brjac.2179-3425.ar-4-2023","DOIUrl":"https://doi.org/10.30744/brjac.2179-3425.ar-4-2023","url":null,"abstract":"The closed-vessel conductively heated digestion system (CHDS) was evaluated to digest dry dog foods for further determination of K, Na, Cu, Fe, Mn, and Zn by high-resolution continuum source flame atomic absorption spectrometry (HR-CS FAAS). The CHDS method was optimized using a fractional factorial design with five variables (HNO3 concentration, H2O2 volume, temperature, holding time, and pre-digestion time) at two levels. The accuracy of the CHDS procedure was checked by the analysis of reference materials from the National Institute of Standards and Technology (NIST SRM 1577b Bovine Liver and 2976 Mussel Tissue) and Brazilian Agricultural Research Corporation (Embrapa MR-E1002A Fish Food). Also, the digestion efficiencies were calculated from residual carbon contents (RCCs). The RCC and blank values in the CHDS digested samples were consistently low, which is suitable for determinations using ICP OES and ICP-MS techniques. For comparison, all samples were also digested by microwave-assisted digestion in closed vessels (MW-AD). Results for Na, K, Cu, Fe, Mn, and Zn determined in sample digests obtained by CHDS were not statistically different at a 95% confidence level from those observed for MW-AD. Limits of quantification (LOQ) calculated from digests in CHDS and MW-AD were comparable, and the values provided adequate limits for elemental determinations in dog foods. Data from mineral composition and moisture were employed in a clustering analysis (HCA) and the discrimination of the samples among different manufacturers and food for dogs at different life stages was possible.","PeriodicalId":9115,"journal":{"name":"Brazilian Journal of Analytical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41650589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-02DOI: 10.30744/brjac.2179-3425.ar-130-2022
Sofia Martins, Giselle de Sousa, Vânia Teles, L. Costa
Cadmium is a toxic element which can be accumulated in the edible parts of plants compromising an entire food chain with serious damage to the living organisms, presenting synergistic and antagonistic effects with the elemental bioaccessibility. In this work, a simulated gastrointestinal digestion was performed to assess the in vitro bioaccessibility of Cd, Cu, Fe and Zn in basil samples after Cd intoxication. The hydroponic cultivation was made in a Hoagland solution at different concentrations (0, 1.5 and 3.0 µmol L-1). Elemental concentration was achieved using a microwave-assisted acid digestion after the growing up of the plants in vermiculite pots by 15 days. The in vitro gastrointestinal procedure was applied in fresh and lyophilized leaves followed by a clean-up step in a sonoreactor cup horn using 1 mL of the extract, 100 µL of HNO3 and 500 µL of H2O2 by 5 minutes. The results showed that Cd bioaccessibility was statistically different at 95% confidence level (p < 0.05) for the lyophilized and fresh leaves samples. The in vitro bioaccessibility increased with concentration in the contamination treatment. Moreover, a high positive correlation was observed between Cd-Fe and Cu-Zn, and a negative correlation between Cd-Zn and Fe-Zn in lyophilized and fresh leaves, respectively, suggesting that the absorption of essential elements was affected by Cd.
{"title":"In vitro bioaccessibility of Cd, Cu, Fe and Zn in basil (Ocimum Basilicum L. Grecco a palla) after cadmium intoxication","authors":"Sofia Martins, Giselle de Sousa, Vânia Teles, L. Costa","doi":"10.30744/brjac.2179-3425.ar-130-2022","DOIUrl":"https://doi.org/10.30744/brjac.2179-3425.ar-130-2022","url":null,"abstract":"Cadmium is a toxic element which can be accumulated in the edible parts of plants compromising an entire food chain with serious damage to the living organisms, presenting synergistic and antagonistic effects with the elemental bioaccessibility. In this work, a simulated gastrointestinal digestion was performed to assess the in vitro bioaccessibility of Cd, Cu, Fe and Zn in basil samples after Cd intoxication. The hydroponic cultivation was made in a Hoagland solution at different concentrations (0, 1.5 and 3.0 µmol L-1). Elemental concentration was achieved using a microwave-assisted acid digestion after the growing up of the plants in vermiculite pots by 15 days. The in vitro gastrointestinal procedure was applied in fresh and lyophilized leaves followed by a clean-up step in a sonoreactor cup horn using 1 mL of the extract, 100 µL of HNO3 and 500 µL of H2O2 by 5 minutes. The results showed that Cd bioaccessibility was statistically different at 95% confidence level (p < 0.05) for the lyophilized and fresh leaves samples. The in vitro bioaccessibility increased with concentration in the contamination treatment. Moreover, a high positive correlation was observed between Cd-Fe and Cu-Zn, and a negative correlation between Cd-Zn and Fe-Zn in lyophilized and fresh leaves, respectively, suggesting that the absorption of essential elements was affected by Cd.","PeriodicalId":9115,"journal":{"name":"Brazilian Journal of Analytical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49536447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-25DOI: 10.30744/brjac.2179-3425.ar-106-2022
Gabriela Alcantara, Giovanna Spíndola, W. Melchert
Coffee contains different volatile and non-volatile compounds, which are responsible for its characteristic flavor and aroma attributes. The extraction of non-volatile compounds from high-quality and traditional coffee by different methods was evaluated to determine the chemical compounds that discriminate between coffees types. Standard methods of preparing the coffee drink by consumers were evaluated. Method A corresponded to boiling water with coffee, and method B to the strained coffee method. Extraction with different solvents did not distinguish the compounds chosen as markers for coffees. In addition to being non-toxic and low-cost, water was the most suitable solvent, conforming to the principles of green chemistry while enabling direct comparison with sensory analysis. The total dissolved solids, percentage extraction, and non-volatile compounds were quantified to select the most satisfactory extraction method. The TDS value ranged from 1.7 to 3 between methods and coffee types, and the extraction percentage ranged from 25 to 45%. Significant differences in the extracts obtained using methods A and B high-quality versus traditional coffees were detected using the Student’s t-test. Although method A extracted the chemical compounds in more substantial amounts, method B was also efficient in extracting the compounds and was easily executed given its similarity to the usual way of preparing coffee beverages used by consumers. The evaluated non-volatile compounds were identified in both high-quality and traditional coffee samples. In the chosen extraction method (method B), the average concentrations in mg 100 g-1 of the sample found for the compounds were: 5-hydroxymethylfurfural (11±0.5), 3,4-hydroxybenzoic acid (62±4), catechin (58±5), 4-hydroxybenzoic acid (58±2), caffeine (1152±44), chlorogenic acid (598±23), caffeic acid (0.7±0.1), and gallic acid (3±0.2).
{"title":"Extraction of Non-Volatile Chemical Compounds in High-Quality and Traditional Coffee","authors":"Gabriela Alcantara, Giovanna Spíndola, W. Melchert","doi":"10.30744/brjac.2179-3425.ar-106-2022","DOIUrl":"https://doi.org/10.30744/brjac.2179-3425.ar-106-2022","url":null,"abstract":"Coffee contains different volatile and non-volatile compounds, which are responsible for its characteristic flavor and aroma attributes. The extraction of non-volatile compounds from high-quality and traditional coffee by different methods was evaluated to determine the chemical compounds that discriminate between coffees types. Standard methods of preparing the coffee drink by consumers were evaluated. Method A corresponded to boiling water with coffee, and method B to the strained coffee method. Extraction with different solvents did not distinguish the compounds chosen as markers for coffees. In addition to being non-toxic and low-cost, water was the most suitable solvent, conforming to the principles of green chemistry while enabling direct comparison with sensory analysis. The total dissolved solids, percentage extraction, and non-volatile compounds were quantified to select the most satisfactory extraction method. The TDS value ranged from 1.7 to 3 between methods and coffee types, and the extraction percentage ranged from 25 to 45%. Significant differences in the extracts obtained using methods A and B high-quality versus traditional coffees were detected using the Student’s t-test. Although method A extracted the chemical compounds in more substantial amounts, method B was also efficient in extracting the compounds and was easily executed given its similarity to the usual way of preparing coffee beverages used by consumers. The evaluated non-volatile compounds were identified in both high-quality and traditional coffee samples. In the chosen extraction method (method B), the average concentrations in mg 100 g-1 of the sample found for the compounds were: 5-hydroxymethylfurfural (11±0.5), 3,4-hydroxybenzoic acid (62±4), catechin (58±5), 4-hydroxybenzoic acid (58±2), caffeine (1152±44), chlorogenic acid (598±23), caffeic acid (0.7±0.1), and gallic acid (3±0.2).","PeriodicalId":9115,"journal":{"name":"Brazilian Journal of Analytical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43809204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-25DOI: 10.30744/brjac.2179-3425.tn-11-2023
Javier Silva, M. Pistón
Biorsorbents can be used to pack minicolumns for the preconcentration of trace elements and thus improve the detection limits of some techniques. A biosorbent is efficient when presents binding sites as carbonyls, amines, and carboxylic groups, among others. Thus, natural materials as the sisal fiber (Agave sisalana) may be a good candidate. Sisal fiber demonstrated good performance for Cu preconcentration when it was impregnated with alizarine fluorine blue (AFB). However, very good results were reported for the first time by our group with this column, without the use of additional reagents for both, Mn and Cu determinations in water samples by using microwave plasma atomic emission spectroscopy (MP AES). In this paper a comparison of the sisal fiber preparation with and without impregnation with AFB is presented and discussed in terms of the figures of merit, including precision, trueness, limits of detection and quantification. In addition to that, the number of determinations without the need of replacement of the solid phase was evaluated. Results demonstrated that the impregnation of sisal fiber with AFB does not leads to an improvement in the analytical performance. Analytical Greenness Metric Approach (AGREE) was used to evaluate the greenness of both methods and results obtained were similar. Despite this, the method without impregnation of the fiber has some remarkable advantages, that contributes to Green Analytical Chemistry (GAC), related to the lifetime of the sorbent, not considered in the AGREE tool.
{"title":"Evaluations of Sisal Fiber as Biosorbent for online Preconcentration and Determination of Cu and Mn coupled to MP AES using the Analytical Greenness Metric Approach","authors":"Javier Silva, M. Pistón","doi":"10.30744/brjac.2179-3425.tn-11-2023","DOIUrl":"https://doi.org/10.30744/brjac.2179-3425.tn-11-2023","url":null,"abstract":"Biorsorbents can be used to pack minicolumns for the preconcentration of trace elements and thus improve the detection limits of some techniques. A biosorbent is efficient when presents binding sites as carbonyls, amines, and carboxylic groups, among others. Thus, natural materials as the sisal fiber (Agave sisalana) may be a good candidate. Sisal fiber demonstrated good performance for Cu preconcentration when it was impregnated with alizarine fluorine blue (AFB). However, very good results were reported for the first time by our group with this column, without the use of additional reagents for both, Mn and Cu determinations in water samples by using microwave plasma atomic emission spectroscopy (MP AES). In this paper a comparison of the sisal fiber preparation with and without impregnation with AFB is presented and discussed in terms of the figures of merit, including precision, trueness, limits of detection and quantification. In addition to that, the number of determinations without the need of replacement of the solid phase was evaluated. Results demonstrated that the impregnation of sisal fiber with AFB does not leads to an improvement in the analytical performance. Analytical Greenness Metric Approach (AGREE) was used to evaluate the greenness of both methods and results obtained were similar. Despite this, the method without impregnation of the fiber has some remarkable advantages, that contributes to Green Analytical Chemistry (GAC), related to the lifetime of the sorbent, not considered in the AGREE tool.","PeriodicalId":9115,"journal":{"name":"Brazilian Journal of Analytical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45660863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-25DOI: 10.30744/brjac.2179-3425.tn-113-2022
Saphire Dias, Aline Oliveira, I. Gaubeur, C. Nomura, J. Naozuka
For physiological functions development and a healthy life maintenance, a balanced diet, containing carbohydrates, proteins, and lipids, should be practiced, and consumed. The proteins should be highlighted, since are essential macronutrients for cell growth and repair mechanisms in our body. There is an increase in the protein sources consumption, mainly by athletes, aiming an increase of muscle mass and to avoid muscle hypertrophy. Although animal proteins exhibit high digestibility, animal foods can not be accessible and widely consumed, due to high cost or lifestyle choice (vegetarians and vegans). In these sceneries, scientists and food industry are constantly searching for alternative proteins, such as plant and fungi proteins. In view of these information, extraction procedures are proposed to proteins fractionation. However, these procedures must be done to exhaustion to guarantee the acquisition of quantitative values. Therefore, the aims of this work were evaluated the protein distribution in edible mushrooms and optimized the sequential protein extraction procedure to obtain total concentration of albumin, globulin, prolamin and glutelin in edible mushrooms, evidencing the need to carry out extraction procedures until exhaustion to adequately attribute nutritional value to edible mushrooms (pink oyster, shiitake, portobello and champignon). The optimized extraction conditions (extractant, time, concentration, number of extractions) were as follows (H2O, 30 min, ---, 3); (NaCl, 15 min, 0.25 mol L-1, 1); (ethanol, 15 min, 50% (v v-1), 1); (NaOH, 60 min, 0.25 mol L-1, 8) for albumin, globulin, prolamin and glutelin extraction. The champignon mushrooms presented all protein group concentrations below LOD and LOQ. The portobello presented the lowest total proteins concentration. The pink oyster mushroom is the species with the highest concentration of albumin and glutelin as well as total protein concentration 4.7 times higher than shiitake mushroom, which is one of the most consumed mushroom species, showing that this exotic species can be promising mainly due to nutritional characteristics and protein source.
{"title":"Optimization of the Protein Sequential Extraction for Quantitative Determination of Albumins, Globulins, Prolamins and Glutelins in Edible Mushrooms","authors":"Saphire Dias, Aline Oliveira, I. Gaubeur, C. Nomura, J. Naozuka","doi":"10.30744/brjac.2179-3425.tn-113-2022","DOIUrl":"https://doi.org/10.30744/brjac.2179-3425.tn-113-2022","url":null,"abstract":"For physiological functions development and a healthy life maintenance, a balanced diet, containing carbohydrates, proteins, and lipids, should be practiced, and consumed. The proteins should be highlighted, since are essential macronutrients for cell growth and repair mechanisms in our body. There is an increase in the protein sources consumption, mainly by athletes, aiming an increase of muscle mass and to avoid muscle hypertrophy. Although animal proteins exhibit high digestibility, animal foods can not be accessible and widely consumed, due to high cost or lifestyle choice (vegetarians and vegans). In these sceneries, scientists and food industry are constantly searching for alternative proteins, such as plant and fungi proteins. In view of these information, extraction procedures are proposed to proteins fractionation. However, these procedures must be done to exhaustion to guarantee the acquisition of quantitative values. Therefore, the aims of this work were evaluated the protein distribution in edible mushrooms and optimized the sequential protein extraction procedure to obtain total concentration of albumin, globulin, prolamin and glutelin in edible mushrooms, evidencing the need to carry out extraction procedures until exhaustion to adequately attribute nutritional value to edible mushrooms (pink oyster, shiitake, portobello and champignon). The optimized extraction conditions (extractant, time, concentration, number of extractions) were as follows (H2O, 30 min, ---, 3); (NaCl, 15 min, 0.25 mol L-1, 1); (ethanol, 15 min, 50% (v v-1), 1); (NaOH, 60 min, 0.25 mol L-1, 8) for albumin, globulin, prolamin and glutelin extraction. The champignon mushrooms presented all protein group concentrations below LOD and LOQ. The portobello presented the lowest total proteins concentration. The pink oyster mushroom is the species with the highest concentration of albumin and glutelin as well as total protein concentration 4.7 times higher than shiitake mushroom, which is one of the most consumed mushroom species, showing that this exotic species can be promising mainly due to nutritional characteristics and protein source.","PeriodicalId":9115,"journal":{"name":"Brazilian Journal of Analytical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43134693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-19DOI: 10.30744/brjac.2179-3425.ar-112-2022
M. Marques, P. Ferreira, Tháles A. Pereira, Alessandra Sussulini
The instrumental aspect employed in metabolomic and lipidomic strategies has been well developed as of the past two decades, allowing large-scale analysis with high sensitivity and reproducibility, while statistical analysis and bioinformatics are emerging fields for data interpretation. Conversely, sample preparation is an important element that, if not optimized, has an impact on metabolome profiling and may result in erroneous and biased biological interpretation, remaining the bottleneck of the metabolomics workflow. Therefore, this work presents an evaluation of liquid-liquid extraction protocols for blood serum samples aiming at metabolomics and lipidomics analysis using liquid chromatography coupled to mass spectrometry (LC-MS). The Bligh & Dyer, Matyash and SIMPLEX protocols were investigated and the findings demonstrate that the composition of the solvents in each approach has a considerable impact on the putatively identified lipids, as well as the average concentration of extracted proteins. The study of the aqueous fraction, however, revealed no statistically significant variations in the identified metabolites. Hence, the optimization of the sample preparation step is crucial for biomolecules profiling and must be carefully considered in accordance with the objectives of the research and the chosen biological matrices.
{"title":"The Importance of Sample Preparation for Omics Analysis: Which Extraction Method is the most Suited for my Biological Question?","authors":"M. Marques, P. Ferreira, Tháles A. Pereira, Alessandra Sussulini","doi":"10.30744/brjac.2179-3425.ar-112-2022","DOIUrl":"https://doi.org/10.30744/brjac.2179-3425.ar-112-2022","url":null,"abstract":"The instrumental aspect employed in metabolomic and lipidomic strategies has been well developed as of the past two decades, allowing large-scale analysis with high sensitivity and reproducibility, while statistical analysis and bioinformatics are emerging fields for data interpretation. Conversely, sample preparation is an important element that, if not optimized, has an impact on metabolome profiling and may result in erroneous and biased biological interpretation, remaining the bottleneck of the metabolomics workflow. Therefore, this work presents an evaluation of liquid-liquid extraction protocols for blood serum samples aiming at metabolomics and lipidomics analysis using liquid chromatography coupled to mass spectrometry (LC-MS). The Bligh & Dyer, Matyash and SIMPLEX protocols were investigated and the findings demonstrate that the composition of the solvents in each approach has a considerable impact on the putatively identified lipids, as well as the average concentration of extracted proteins. The study of the aqueous fraction, however, revealed no statistically significant variations in the identified metabolites. Hence, the optimization of the sample preparation step is crucial for biomolecules profiling and must be carefully considered in accordance with the objectives of the research and the chosen biological matrices.","PeriodicalId":9115,"journal":{"name":"Brazilian Journal of Analytical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43548632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-19DOI: 10.30744/brjac.2179-3425.rv-136-2022
Hanna de Sá, E. D. dos Santos, Samara Carvalho, Rafaela Nunes, G. Canuto
Metabolomics has become a prominent area within the omics sciences and allows an understanding of complex biological systems. Among several areas of knowledge, the study of microorganisms (microbial metabolomics) has received attention. Due to the many species of microorganisms and their high metabolic complexity, many challenges are involved in metabolomics workflow. Careful experimental design and execution of the experiments will provide reliable results, allowing correct biological interpretation. This review presents the fundamentals of metabolomics and workflow, focusing on the description of the steps and analytical strategies applied to microbial sample preparation, highlighting the current challenges in sample handling. In addition, the state of the art of analytical technologies based on separation techniques hyphenated to mass spectrometry and applications in microbial metabolomics are presented.
{"title":"Fundamentals and Analytical Strategies for Metabolomics Workflow: An Overview and Microbial Applications","authors":"Hanna de Sá, E. D. dos Santos, Samara Carvalho, Rafaela Nunes, G. Canuto","doi":"10.30744/brjac.2179-3425.rv-136-2022","DOIUrl":"https://doi.org/10.30744/brjac.2179-3425.rv-136-2022","url":null,"abstract":"Metabolomics has become a prominent area within the omics sciences and allows an understanding of complex biological systems. Among several areas of knowledge, the study of microorganisms (microbial metabolomics) has received attention. Due to the many species of microorganisms and their high metabolic complexity, many challenges are involved in metabolomics workflow. Careful experimental design and execution of the experiments will provide reliable results, allowing correct biological interpretation. This review presents the fundamentals of metabolomics and workflow, focusing on the description of the steps and analytical strategies applied to microbial sample preparation, highlighting the current challenges in sample handling. In addition, the state of the art of analytical technologies based on separation techniques hyphenated to mass spectrometry and applications in microbial metabolomics are presented.","PeriodicalId":9115,"journal":{"name":"Brazilian Journal of Analytical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46837926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：