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Detection of anti-Toxoplasma gondii antibodies in wild free-living birds and mammals from the northwest region of São Paulo state, Brazil 巴西<s:1>圣保罗州西北地区野生鸟类和哺乳动物刚地弓形虫抗体检测
Q3 Veterinary Pub Date : 2021-08-10 DOI: 10.11606/issn.1678-4456.bjvras.2021.176683
L. Castiglioni, L. Aires, Vinícius Matheus Ferrari, F. Murata, H. Soares, S. Gennari, J. G. Machado, Adriana Santiago Fracischetti, Ricardo Quiterio Sartori, Luiz Carlos de Mattos, C. Brandão, H. Pena
Toxoplasmosis is a protozoonosis caused by an obligate intracellular parasite named Toxoplasma gondii, which can infect humans and a large number of homeothermic animal species with worldwide distribution. The present study aimed to detect anti T. gondii antibodies from serological samples of free living wild animals from the northwest region of São Paulo state, Brazil. Thirty-two samples (eight from birds and 24 from mammals) were analyzed by the modified agglutination test (MAT) using 5 cut-off points for birds and 25 for mammals. Seropositivity was observed in 25% (2/8) of birds, including the species Rupornis magnirostris (roadside hawk) and Caracara plancus (southern caracara), and 29.2% (7/24) animals were seropositive among mammals, including one hoary fox (Lycalopex vetulus), two maned wolves (Chrysocyon brachyurus), one black howler monkey (Alouatta caraya), two crab-eating foxes (Cerdocyon thous) and one gray brocket deer (Mazama gouazoubira). The results obtained with the present study indicate the exposure to T. gondii of free-living wild animals from the northwest region of São Paulo state and, therefore, that they probably play a role in the transmission and maintenance of T. gondii in the environment they inhabit. Thus, identification of the infection in several animal species in the region indicates the environmental contamination of the area. Studies of this nature may help to understand the importance of the prevention and control of this disease in Brazil.
弓形虫病是由一种名为弓形虫的专性细胞内寄生虫引起的一种原体病,它可以感染人类和大量分布在世界各地的恒温动物。本研究旨在从巴西圣保罗州西北地区的自由生活野生动物的血清学样本中检测抗弓形虫抗体。32个样本(8个来自鸟类,24个来自哺乳动物)通过改良凝集试验(MAT)进行分析,其中鸟类使用5个截止点,哺乳动物使用25个截止点。在25%(2/8)的鸟类中观察到血清阳性,包括Rupornis magnirostris(路鹰)和Caracara plancus,两只吃螃蟹的狐狸(Cerdocyon thou)和一只灰色的小鹿(Mazama gouazoubira)。本研究的结果表明,来自圣保罗州西北地区的自由生活野生动物暴露于弓形虫,因此,它们可能在弓形虫在其栖息环境中的传播和维持中发挥了作用。因此,该地区几种动物的感染表明该地区的环境受到污染。这种性质的研究可能有助于理解巴西预防和控制这种疾病的重要性。
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引用次数: 0
Erratum: Investigation of Feline calicivirus infection in cats with upper respiratory tract disease in Diyarbakir, Turkey 勘误表:土耳其迪亚巴克尔猫上呼吸道疾病猫杯状病毒感染的调查
Q3 Veterinary Pub Date : 2021-08-10 DOI: 10.11606/issn.1678-4456.bjvras.2021.188699
Nazan Baksi, A. Simsek
Feline calicivirus is among the most common pathogenic microorganisms in upper respiratory tract disease (URTD) and oral lesions of cats. It leads to stomatitis, oral ulceration, ocular and nasal discharge, conjunctivitis, fever, lameness, anorexia, hypersalivation, pneumonia, respiratory distress, coughing, and depression in infected cats. This study aimed to determine the role of Feline calicivirus (FCV) in cats with the upper respiratory tract disease in the Diyarbakir region, Turkey, to provide treatment for infected cats and contribute to the disease prophylaxis. The study material consisted of 10 cats (control group) considered to be healthy according to the clinical examination and 20 cats with URTD that were not vaccinated against Feline calicivirus infection of different breeds, ages, and genders brought to Dicle University Veterinary Faculty Prof. Dr. Servet SEKIN Polyclinic with URTD. After routine clinical examinations of the animals, oral and conjunctival swabs and blood samples were taken. Hematological and biochemical analyzes of blood samples were performed. Swab samples were analyzed by the polymerase chain reaction (PCR) method for the diagnosis of the agent. Oral lesions, hypersalivation, ocular and nasal discharge, coughing, and breathing difficulties were seen in clinical examinations of cats with URTD. Feline calicivirus was detected in only one cat's conjunctival swab sample in PCR analyses. As a result, we found that Feline calicivirus infection was present in cats with URTD in the Diyarbakir region, and 5% positivity was found in cats with clinical symptoms according to PCR analysis.
猫杯状病毒是猫上呼吸道疾病(URTD)和口腔病变最常见的病原微生物之一。它会导致受感染猫出现口炎、口腔溃疡、眼鼻分泌物、结膜炎、发热、跛行、厌食、唾液分泌过多、肺炎、呼吸窘迫、咳嗽和抑郁。本研究旨在确定猫杯状病毒(FCV)在土耳其迪亚巴克尔地区患有上呼吸道疾病的猫中的作用,为受感染的猫提供治疗并有助于疾病预防。研究材料包括10只经临床检查认为健康的猫(对照组)和20只未接种猫杯状病毒感染疫苗的不同品种、年龄和性别的患有URTD的猫,这些猫被带到Dicle大学兽医学院Servet SEKIN博士教授的URTD综合诊所。在对动物进行常规临床检查后,采集口腔和结膜拭子及血液样本。对血样进行血液学和生化分析。采用聚合酶链反应(PCR)法对拭子样本进行诊断。口腔病变、唾液分泌过多、眼和鼻分泌物、咳嗽和呼吸困难在患有URTD的猫的临床检查中可见。在PCR分析中,仅在一只猫的结膜拭子样本中检测到猫杯状病毒。结果,我们发现在迪亚巴克尔地区患有URTD的猫中存在猫杯状病毒感染,根据PCR分析,在有临床症状的猫中发现5%的阳性。
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引用次数: 0
Serological survey of leptospirosis, brucellosis, and lentivirus in herds of small ruminants in Recôncavo Baiano, Bahia, Brazil 巴西巴伊亚州Recôncavo Baiano小型反刍动物群中钩端螺旋体病、布鲁氏菌病和慢病毒的血清学调查
Q3 Veterinary Pub Date : 2021-07-27 DOI: 10.11606/issn.1678-4456.bjvras.2021.180290
Rodrigo Rezende Mires de Carvalho, L. N. Paz, C. Dias, Giselle Almeida Nocera, Augusto José Pereira de Mesquita, M. Pinna
A serological survey was carried out to assess the frequency of leptospirosis, small ruminants lentivirus (SRLV), and brucellosis in small ruminant herds in the Recôncavo Baiano, State of Bahia, Brazil, from February to December 2017. In four goat herds, 125 animals were tested for SRLV and leptospirosis, while in five sheep herds, 378 animals were tested for leptospirosis, brucellosis, and SRLV. Regarding leptospirosis, MAT detected 80% of goats and 15.34% of sheep seroreactive. Reactivity was most frequent to serogroups Autumnalis and Grippotyphosa in goats and sheep, respectively. Regarding SRLV, 8.8% of goats and 0.79% of sheep were reactive. Search for anti-B. ovis antibodies revealed 0.52% reactivity. In sheep, three animals showed simultaneous seroreactivity for SRLV and leptospirosis, while one animal had a serological response for brucellosis and leptospirosis. In goats, simultaneous seroreactivity for SRLV and leptospirosis was observed in only one animal. Leptospirosis was the most frequent of the three infectious diseases in investigated herds.
2017年2月至12月,对巴西巴伊亚州Recôncavo Baiano的小型反刍动物群中钩端螺旋体病、小型反刍动物慢病毒(SRLV)和布鲁氏菌病的发病率进行了血清学调查。在四个山羊群中,对125只动物进行了SRLV和钩端螺旋体病检测,而在五个绵羊群中,378只动物接受了钩端螺旋菌病、布鲁氏菌病和SRLV检测。关于钩端螺旋体病,MAT检测到80%的山羊和15.34%的绵羊血清反应性。山羊和绵羊对Autumnalis血清群和Grippotyphosa血清群的反应最为频繁。关于SRLV,8.8%的山羊和0.79%的绵羊具有反应性。搜索抗-B。ovis抗体的反应性为0.52%。在绵羊中,三只动物对SRLV和钩端螺旋体病同时表现出血清反应性,而一只动物对布鲁氏菌病和钩端螺体病具有血清反应性。在山羊中,只有一只动物同时出现SRLV和钩端螺旋体病的血清反应性。钩端螺旋体病是调查牛群中最常见的三种传染病。
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引用次数: 0
Fatty acids profile, atherogenic and thrombogenic health lipid indices in the meat of lambs that received canola grain 食用油菜籽粒的羔羊肉中的脂肪酸分布、动脉粥样硬化和血栓形成的健康脂质指数
Q3 Veterinary Pub Date : 2021-07-20 DOI: 10.11606/ISSN.1678-4456.BJVRAS.2021.178023
Mayara Mitiko Yoshihara Carneiro, R. Goes, B. Barros, R. T. Oliveira, A. R. M. Fernandes, N. G. Silva, D. G. Anschau, C. Cardoso, Sullyvan Silva Oliveira, Yasmin dos Santos Picanço
This study evaluated the fatty acid composition and qualitative characteristics of meat from lambs feed in feedlot with increasing levels of canola grain. Meat obtained from 27 lambs fed 0, 8 and 16% inclusion of canola grain were analyzed. There was a reduction in the content of saturated fatty acids (SFA): lauric, heptadecanoic, and stearic; and unsaturated fatty acids (UFA): palmitoleic, oleic, and eicosatrienoic, with the increasing levels of canola grain. There was a linear reduction for the ω3 acid and the ω3: ω6 ratio, while the ω6: ω3 ratio increased. But the values observed for this ratio (ω6: ω3) were lower than 4, which is considered a satisfactory value. There was a slight increase (~2%) for the thrombogenicity index and atherogenicity index with the inclusion of canola grain. There was a reduction in the hypocholesterolemic: hypercholesterolemic ratio, with mean values of 2.09 for the diet with 0% inclusion, and 2.06 for 8 and 16% inclusion of canola. Although the inclusion of canola grain for lambs decreased the content of some UFA, reflecting alterations in the correlated nutritional properties, there is a reduction of SFA. The results for instrumental analysis, proximate composition, and sensory acceptance of the lamb meat were similar among the treatments.
本研究评估了随着油菜籽含量的增加,饲养场羔羊肉的脂肪酸组成和质量特征。对27只饲喂0、8%和16%油菜籽的羔羊肉进行了分析。饱和脂肪酸(SFA)含量减少:月桂酸、十七烷酸和硬脂酸;和不饱和脂肪酸(UFA):棕榈油酸、油酸和二十碳三烯酸,随着油菜籽粒含量的增加。ω3酸和ω3∶ω6的比例呈线性下降,而ω6∶ω3的比例增加。但观察到的这个比值(ω6:ω3)低于4,这被认为是一个令人满意的值。随着油菜籽颗粒的加入,血栓形成指数和动脉粥样硬化指数略有增加(约2%)。低胆固醇血症与高胆固醇血症的比率有所降低,0%的饮食平均值为2.09,8%和16%的油菜籽平均值为2.006。尽管为羔羊添加菜籽油降低了一些UFA的含量,反映了相关营养特性的变化,但SFA也有所降低。不同处理的羊肉的仪器分析、接近成分和感官接受度结果相似。
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引用次数: 6
Effect of cryoprotectant on the motility, viability, fertilization, and DNA integrity of naleh fish Barbonymus sp. (Cyprinidae) sperm 低温保护剂对鲤科Barbonymus sp.精子活力、活力、受精和DNA完整性的影响
Q3 Veterinary Pub Date : 2021-07-16 DOI: 10.11606/ISSN.1678-4456.BJVRAS.2021.168702
S. Maulida, K. Eriani, F. Nur, N. Fadli, A. Batubara, A. A. Muhammadar, M. N. Siti-Azizah, M. Wilkes, Z. Muchlisin
Naleh fish Barbonymus sp. is a commercial freshwater fish, which is indigenous to Aceh, Indonesia. The population of this species has declined over the years as a result of habitat perturbations and overfishing. Hence, the crucial need to develop a cryopreservation method to support breeding programs. This involved the use of a cryoprotectant as an important component. The objective of this study, therefore, was to explore the best cryoprotectant for naleh fish spermatozoa, and a total of five types were tested. These include the DMSO, Methanol, Ethanol, Glycerol, and Ethylene Glycol at a similar concentration of 10%, which were individually combined with 15% egg yolk, and every treatment was performed in three replications. Conversely, Ringer’s solution was adopted as an extender, and the sperm was cryopreserved in liquid nitrogen for 15 days. The results showed significant influence on sperm motility and viability, as well as egg fertility of naleh fish (P <0.05), although the DMSO provided the best outcome, compared to others at 47.17%, 50.13%, and 45.67%, respectively. Furthermore, DNA fragmentation had not occurred in the fresh and cryopreserved sperm samples, indicating the protective effect of tested cryoprotectants. It is concluded that the 10% DMSO and 15% egg yolk is the best cryoprotectant for naleh fish spermatozoa.
Barbonymus sp.是一种商业淡水鱼,原产于印度尼西亚亚齐。由于栖息地的扰动和过度捕捞,这一物种的数量多年来一直在下降。因此,迫切需要开发一种冷冻保存方法来支持育种计划。这涉及到使用冷冻保护剂作为一个重要的组成部分。因此,本研究的目的是探索最适合纳勒鱼精子的冷冻保护剂,共测试了五种类型。其中包括相似浓度为10%的DMSO、甲醇、乙醇、甘油和乙二醇,分别与15%的蛋黄混合,每次处理进行3个重复。反之,用林格液作填充剂,将精子在液氮中低温保存15天。结果显示,DMSO对naleh鱼的精子活力、活力和卵子生育能力有显著影响(P <0.05),但DMSO的效果最好,分别为47.17%、50.13%和45.67%。此外,在新鲜和冷冻保存的精子样本中没有发生DNA断裂,表明所测试的冷冻保护剂具有保护作用。综上所述,10%的DMSO和15%的蛋黄是对纳利鱼精子最好的冷冻保护剂。
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引用次数: 4
Epidemiological characteristics of Leptospira spp. infection in bovine herds in Mato Grosso do Sul, Brazil 巴西南马托格罗索州牛群钩端螺旋体感染的流行病学特征
Q3 Veterinary Pub Date : 2021-07-16 DOI: 10.11606/ISSN.1678-4456.BJVRAS.2021.180127
R. F. Santos, D. Frias, G. Silva, Talita Ribeiro Silva, N. A. Assis, C. R. Bastos, Vanessa Felipe de Souza, L. Mathias
The epidemiological characteristics of bovine leptospirosis in animals and herds in Mato Grosso do Sul were investigated to determine parameters such as disease frequency and the serovars reactant in beef cattle herds. A total of 4,629 beef cattle herds were examined against 33 Leptospira spp. serovars. The serum samples were submitted to the microscopic agglutination test (MAT) for the serological diagnosis of leptospirosis. The MAT results showed that 3,814 (82.39%) of the 4,629 animals evaluated were seropositive for the bacterium, with serological reactions mainly to serogroup Sejroe, serovar Wolffi (36.49%). The observed high frequency of reactive animals demonstrates the relevance of the infection. Therefore, general and specific measures should be implemented to contain and/or prevent infection of the animals in the studied region.
研究了南马托格罗索州动物和牛群中牛钩端螺旋体病的流行病学特征,以确定牛群中的疾病频率和血清反应物等参数。对4629个肉牛群进行了33种钩端螺旋体血清型的检测。将血清样本进行显微镜凝集试验(MAT),用于钩端螺旋体病的血清学诊断。MAT结果显示,在评估的4629只动物中,3814只(82.39%)对该细菌呈血清阳性,血清学反应主要针对血清群Sejroe、血清型Wolffi(36.49%)。观察到的高频率反应动物表明了感染的相关性。因此,应采取一般和具体措施来控制和/或预防研究区域内动物的感染。
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引用次数: 0
De acordo com o governador, a decisão vale para todos que estejam em condições físicas adequadas, mesmo os que ainda não tomaram a vacina contra a Covid-19 根据州长的说法,这一决定适用于所有身体状况合适的人,即使是那些尚未接种Covid-19疫苗的人
Q3 Veterinary Pub Date : 2021-07-14 DOI: 10.11606/ISSN.1678-4456.BJVRAS.2021.181002
Yudit Rodríguez Coipel, Aníbal Domínguez Odio, Odaimis Mena Álvarez, Raiselys Toirac Proenza, Isbel González Marrero, Daniel Leonardo Cala Delgado
The industrial manufacturing of natural products for veterinary use represents a major weakness in the veterinary sector despite increased interest and the traditional ancestral knowledge that supports them. A cross-sectional, descriptive observational study was conducted to characterize the veterinary herbal products marketed worldwide during 2018 and 2019. A comprehensive thematic search limited to the 2018-2019 period was performed in the ScienceDirect, Scopus, PubMed, Web of Science, ResearchGate, and Academic Search Complete databases. The investigation identified 487 products registered in the global market manufactured by 54 companies, led by India, The Netherlands, and the United Kingdom. The market segments of animal production and phytomedicines were dominant with 73.7% and 53.0% of products, respectively. Cattle (22.2%), sheep-goats (16.2%), and canines (16.2%) were the most favored species. The most represented therapeutic indications were those intended to treat gastrointestinal disorders (30.47%), antimicrobials (16.66%), and antiparasitic agents (10.47%). The families Fabaceae, Lamiaceae, Asteraceae, Apiaceae, Malvaceae, and Rutaceae stood out because of their frequent use, encompassing 35.0% of the 137 declared species. Andrographis paniculata (Burm.f.) Nees and Withania somnifera (Lin) Dunal were the most important species. Oral formulations for internal use (72%) and liquids (51%) in 100 mL, 500 mL, and 1 L presentations showed the highest prevalence on the market. The global market for veterinary herbal products during the 2018-2019 period was relevant in the productive and medical animal sector. No differences were found between medicinal plant species used to formulate herbal products for human and animal use.
兽医用天然产品的工业制造是兽医部门的一个主要弱点,尽管人们对其越来越感兴趣,传统的祖先知识也为其提供了支持。进行了一项横断面描述性观察性研究,以确定2018年和2019年全球销售的兽医草药产品的特征。在ScienceDirect、Scopus、PubMed、Web of Science、ResearchGate和Academic search Complete数据库中进行了仅限于2018-2019年期间的全面主题搜索。调查确定了以印度、荷兰和英国为首的54家公司在全球市场上注册的487种产品。动物生产和植物药物市场份额占主导地位,分别占73.7%和53.0%。牛(22.2%)、绵羊-山羊(16.2%)和犬科动物(162%)是最受青睐的物种。最具代表性的治疗适应症是那些旨在治疗胃肠道疾病的适应症(30.47%)、抗菌药物(16.66%)和抗寄生虫药物(10.47%)。蚕豆科、Lamiaceae、菊科、Apeaceae、锦葵科和芸香科因其频繁使用而脱颖而出,占137个申报物种的35.0%。穿心莲(Andrographis paniculata,Burm.f.)Nees和紫檀(Withania somnifera,Lin)Dunal是最重要的物种。100毫升、500毫升和1升的内服口服制剂(72%)和液体(51%)在市场上的流行率最高。2018-2019年期间,全球兽医草药产品市场与生产和医疗动物部门相关。用于配制供人类和动物使用的草药产品的药用植物物种之间没有发现差异。
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引用次数: 1
Occurrence of Leptospira spp. and factors associated with the infection in horses from a military contingent in the Rio Grande do Sul State, Brazil 巴西南大德州某军事特遣队马匹中钩端螺旋体的发生及与感染相关的因素
Q3 Veterinary Pub Date : 2021-07-14 DOI: 10.11606/ISSN.1678-4456.BJVRAS.2021.180884
Mariane Pacheco Bastiani, L. Lovato, A. V. von Laer, L. Pötter, R. Rodrigues, Bianca Costabile de Souza, G. P. Herrmann, L. A. Sangioni, S. Botton
The seroprevalence of Leptospira spp. and infection risk factors were investigated in horses from a military contingent in the Rio Grande do Sul State, Brazil. A total of 446 horses were evaluated and categorized into 309 mares, 11 stallions, and 126 horses with an average age of three years. To determine seroprevalence, serum samples from all horses were submitted to the microscopic agglutination test against 12 serovars belonging to nine serogroups, usually circulating in equine populations. To investigate the possible risk factors of infection by Leptospira spp., questionnaires were applied in that military unit. The seroprevalence in the horses with an average age of 3 years, mares, and stallions were 57.94% (73/126), 54.05% (165/309), and 45.45% (5/11), respectively. The main risk factors identified were contact with different water sources, the presence of rodents, and contact with wild and domestic animals. The high seroprevalence of anti‑Leptospira spp. antibodies may be associated with the presence of these risk factors. Therefore, the exposure of horses to possible reservoirs of Leptospira spp. should be minimized. Also, the immunoprophylaxis protocol should be reviewed and a shorter interval between vaccinations adopted to control leptospirosis in this herd. 
研究了巴西南大德州某军事特遣队马的钩端螺旋体血清流行率和感染危险因素。共有446匹马被评估,分为309匹母马、11匹公马、126匹平均年龄为3岁的马。为了确定血清阳性率,将所有马的血清样本提交给显微镜凝集试验,检测属于9个血清组的12种血清型,这些血清型通常在马种群中流行。为调查该部队钩端螺旋体感染的可能危险因素,采用问卷调查方法。平均年龄为3岁的马、母马和种马的血清阳性率分别为57.94%(73/126)、54.05%(165/309)和45.45%(5/11)。确定的主要危险因素是与不同水源的接触、啮齿动物的存在以及与野生动物和家畜的接触。抗钩端螺旋体抗体的高血清阳性率可能与这些危险因素的存在有关。因此,应尽量减少马与可能的钩端螺旋体宿主的接触。此外,应审查免疫预防方案,并缩短疫苗接种间隔,以控制该群体的钩端螺旋体病。
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引用次数: 5
Buffaloes (Bubalus bubalis) mycotic eye infection: reported herd cases in Thi-Qar province, Iraq 水牛(Bubalus bubalis)眼部真菌感染:伊拉克Thi-Qar省报告的牛群病例
Q3 Veterinary Pub Date : 2021-07-14 DOI: 10.11606/ISSN.1678-4456.BJVRAS.2021.180221
J. M. Khalaf, A. Karim, Karima Akool Al Salihi
Buffaloes are one of the important farm animals in the south of Iraq and play an essential economical role mainly acting as dairy, meat, and draft animals. This study intended to diagnose buffalo mycotic eye infections in Thi-Qar province/Iraq. Some 250 buffaloes in the herd of 3,700 animals suffered from eye infections from December 2017 to November 2018. Eye swabs were collected from each infected eye of the affected buffaloes of both sexes before treatment. The animals were in different age groups. All samples were transferred to the laboratory in transfer media, and cultured on Sabouraud dextrose (SDA) agar with and without 0.05 g/mL and 0.4 g/mL chloramphenicol and cycloheximide, respectively. Later, the agars were incubated at 25o C and 37o C. The total percentage of eye infection was (6.75%), constituting (49.2%) mycotic infections. The predominant clinical manifestations that appeared on the infected buffaloes were eye inflammation represented by congestion, lacrimation, the opacity of cornea and edema, and reduced productivity of the infected animals. Different fungal isolates were identified from the samples including Aspergillus fumigates, Aspergillus flavus, Aspergillus niger, Penicillium spp., Alternaria spp., Fusarium spp., Candida spp., Cladosporium spp., Rhodotorula spp., Mucor spp. and Rhizopus spp. Calves buffaloes below one-year-old were more prone to mycotic infection than one-year-old or more. Additionally, male buffaloes were more susceptible to infection than females. In conclusion, this study isolated various types of fungus from the inflamed eyes of buffaloes. Fungal eye infection and the potential risk factors for fungal keratitis in buffaloes were also investigated. The study also approved the rapid diagnosis of fungi by direct microscopic detection and culture. The author recommends future studies including large numbers of the buffalo herd in Iraq to determine the epidemiology of this condition in the country. 
水牛是伊拉克南部重要的农场动物之一,在经济上发挥着重要作用,主要作为乳制品、肉类和饲养动物。本研究旨在诊断伊拉克锡卡尔省水牛真菌性眼部感染。2017年12月至2018年11月,3700只水牛中约有250只水牛眼睛感染。在治疗前,从受感染的两性水牛的每只眼睛中采集眼拭子。这些动物属于不同的年龄组。将所有样品在转移培养基中转移到实验室,并分别在含有和不含有0.05g/mL和0.4g/mL氯霉素和环己酰亚胺的沙氏葡萄糖(SDA)琼脂上培养。随后,将琼脂在25℃和37℃下孵育。眼睛感染的总百分比为(6.75%),构成(49.2%)真菌感染。感染水牛的主要临床表现是眼部炎症,表现为充血、流泪、角膜混浊和水肿,以及感染动物的生产力下降。从样品中鉴定出不同的真菌分离株,包括烟曲霉、黄曲霉、黑曲霉、青霉、链格孢菌、镰刀菌、念珠菌、枝孢菌、红酵母、毛霉和根霉。此外,雄性水牛比雌性水牛更容易感染。总之,本研究从水牛发炎的眼睛中分离出了各种类型的真菌。还对水牛真菌性眼感染和真菌性角膜炎的潜在危险因素进行了研究。该研究还批准了通过直接显微镜检测和培养快速诊断真菌。作者建议未来对伊拉克大量水牛群进行研究,以确定该国这种疾病的流行病学。
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引用次数: 2
Surgical correction of bilateral ectopic ureter using the neoureterocystostomy technique 应用神经输尿管膀胱造口术矫正双侧输尿管异位
Q3 Veterinary Pub Date : 2021-07-13 DOI: 10.11606/ISSN.1678-4456.BJVRAS.2021.182499
Ana Oliveira, Angélica Andrade Guimarães, Thais Lisboa Machado Romano, Daniel Herreira Jarrouge, D. S. Angrimani
Ureteral ectopy is a rare disorder in the small animals’ clinic. It is characterized as a congenital anomaly, resulting from the ducts differentiation failure during embryogenesis. In this scenario, the ureters present themselves outside the anatomical site, being inserted into the uterus, urethra, urinary vesicle neck, or vagina. The clinical signs are urinary incontinence and perivulvar dermatitis. Surgery is the accepted treatment to correct the anomaly. The surgical procedure is based on relocating the ectopic ureter and treating associated modifications. This report describes a case of intramural bilateral ureteral ectopy, corrected surgically through the neoureterocystostomy technique, making it possible to control the animal’s urinary incontinence.
输尿管异位是小动物临床中一种罕见的疾病。它的特征是一种先天性异常,是由胚胎发育过程中导管分化失败引起的。在这种情况下,输尿管出现在解剖部位外,插入子宫、尿道、尿囊颈或阴道。临床表现为尿失禁和外阴周围皮炎。手术是矫正畸形的公认治疗方法。手术是基于重新定位异位输尿管和治疗相关的改变。本报告描述一例双侧输尿管内异位,通过神经输尿管膀胱造口技术进行手术矫正,使控制动物尿失禁成为可能。
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引用次数: 0
期刊
Brazilian Journal of Veterinary Research and Animal Science
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