Pub Date : 2021-07-13DOI: 10.11606/ISSN.1678-4456.BJVRAS.2021.175224
L. C. Carneiro, E. Williams, J. Saut, R. Santos, E. Celeghini
In dairy cattle, supplementation with polyunsaturated fatty acids (PUFAs) is considered to be an important tool to decrease the negative energy balance of periparturient dairy cows and improve the reproductive and immune systems. The most common PUFAs added to ruminant diets are omega 3 (n-3 PUFA) as linolenic acid and omega 6 (n-6 PUFA) as linoleic acid. This paper aims to review the potential effects of n-3 PUFA. We consider the effects of n-3 PUFA on the bovine immune system, especially on immune cells, and on in vivo and in vitro reproductive parameters, emphasizing how n-3 PUFAs act as modulators through one or more molecular mechanisms. The incorporation of n-3 PUFA in the dairy cow diet has positive effects on animal fertility and immunity. Future research on n-3 PUFA should be more explored concerning reproduction and immune function, starting from the investigation of basic biology to their potential for application in the clinical and preventive medicine fields.
{"title":"The effect of n- 3 polyunsaturated fatty acid supplementation on immune and reproductive parameters in dairy cows","authors":"L. C. Carneiro, E. Williams, J. Saut, R. Santos, E. Celeghini","doi":"10.11606/ISSN.1678-4456.BJVRAS.2021.175224","DOIUrl":"https://doi.org/10.11606/ISSN.1678-4456.BJVRAS.2021.175224","url":null,"abstract":"In dairy cattle, supplementation with polyunsaturated fatty acids (PUFAs) is considered to be an important tool to decrease the negative energy balance of periparturient dairy cows and improve the reproductive and immune systems. The most common PUFAs added to ruminant diets are omega 3 (n-3 PUFA) as linolenic acid and omega 6 (n-6 PUFA) as linoleic acid. This paper aims to review the potential effects of n-3 PUFA. We consider the effects of n-3 PUFA on the bovine immune system, especially on immune cells, and on in vivo and in vitro reproductive parameters, emphasizing how n-3 PUFAs act as modulators through one or more molecular mechanisms. The incorporation of n-3 PUFA in the dairy cow diet has positive effects on animal fertility and immunity. Future research on n-3 PUFA should be more explored concerning reproduction and immune function, starting from the investigation of basic biology to their potential for application in the clinical and preventive medicine fields.","PeriodicalId":9119,"journal":{"name":"Brazilian Journal of Veterinary Research and Animal Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64845415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-12DOI: 10.11606/ISSN.1678-4456.BJVRAS.2021.182579
Sergio Andrés Vélez Gil, Juan José Patiño Marulanda, J. R. Martínez Aranzales
Enteroliths are concretions of minerals that cause partial or total obstruction of the intestinal lumen, resulting in recurrent and chronic colic in horses. This pilot study aimed to evaluate the in vitro solvent effect of carbonated beverages (Coca-Cola® and Coca-Cola® Zero), and papain and cellulase enzymes (Robinson Pharma®, Santa Ana, CA, USA) on enteroliths obtained from horses. Six 51-grams samples of six enteroliths were assigned to six treatments of immersion solutions: T1, Coca-Cola®; T2: Coca-Cola® Zero; T3: distilled water + papain (90 mg) and cellulase (120 mg); T4: Coca-Cola® + papain and cellulase; T5: Coca-Cola® Zero + papain and cellulase; and, CT: distilled water (control). The volume for immersion in the assigned solution was 150 mL, at a pH of 7.1, using an incubation shaker (Heidolph® , Germany) at 37ºC and 25 rpm, for 72 h. The evaluation periods of the dissolution percentage (difference between the initial weight and final weight of the samples), were 0, 3, 12, 24, 36, 48, 60, and 72 h. After 72 h of immersion, solutions T4, T5, and T1 presented 47, 38.8, and 14.9% of dissolution, respectively. The other solutions did not have major differences with CT (control). Under the in vitro conditions of this pilot study, papain and cellulase enzymes potentiated the dissolving effect of the carbonated solutions on the enteroliths obtained from horses. Further studies are suggested since the existing literature is on the dissolution of phytobezoars and not of enteroliths.
{"title":"In vitro evaluation of the dissolving effect of carbonated beverages (Coca-Cola® ) and enzyme-based solutions on enteroliths obtained from horses: pilot study","authors":"Sergio Andrés Vélez Gil, Juan José Patiño Marulanda, J. R. Martínez Aranzales","doi":"10.11606/ISSN.1678-4456.BJVRAS.2021.182579","DOIUrl":"https://doi.org/10.11606/ISSN.1678-4456.BJVRAS.2021.182579","url":null,"abstract":"Enteroliths are concretions of minerals that cause partial or total obstruction of the intestinal lumen, resulting in recurrent and chronic colic in horses. This pilot study aimed to evaluate the in vitro solvent effect of carbonated beverages (Coca-Cola® and Coca-Cola® Zero), and papain and cellulase enzymes (Robinson Pharma®, Santa Ana, CA, USA) on enteroliths obtained from horses. Six 51-grams samples of six enteroliths were assigned to six treatments of immersion solutions: T1, Coca-Cola®; T2: Coca-Cola® Zero; T3: distilled water + papain (90 mg) and cellulase (120 mg); T4: Coca-Cola® + papain and cellulase; T5: Coca-Cola® Zero + papain and cellulase; and, CT: distilled water (control). The volume for immersion in the assigned solution was 150 mL, at a pH of 7.1, using an incubation shaker (Heidolph® , Germany) at 37ºC and 25 rpm, for 72 h. The evaluation periods of the dissolution percentage (difference between the initial weight and final weight of the samples), were 0, 3, 12, 24, 36, 48, 60, and 72 h. After 72 h of immersion, solutions T4, T5, and T1 presented 47, 38.8, and 14.9% of dissolution, respectively. The other solutions did not have major differences with CT (control). Under the in vitro conditions of this pilot study, papain and cellulase enzymes potentiated the dissolving effect of the carbonated solutions on the enteroliths obtained from horses. Further studies are suggested since the existing literature is on the dissolution of phytobezoars and not of enteroliths. ","PeriodicalId":9119,"journal":{"name":"Brazilian Journal of Veterinary Research and Animal Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41388113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-08DOI: 10.11606/ISSN.1678-4456.BJVRAS.2021.183219
G. Mothé, N. F. Reis, C. S. I. Melivilu, Aguinaldo Francisco Mendes Junior, Cinthia S Dos Santos, A. Dieckmann, R. L. Machado, Elisabeth M. S. Rocha, A. Baptista
Sporotrichosis is a dermatozoonosis, caused by dimorphic pathogenic fungi of the genus Sporothrix. Although Sporothrix brasiliensis is the most frequent and pathogenic species identified from the Brazilian sporotrichosis hyperendemic, to the best of our knowledge this is the first report of its molecular diagnosis from a cat with ocular lesions. A 3-month old female, domestic feline presented an ocular manifestation with granuloma in the lower-left palpebral conjunctiva, in addition to mucocutaneous lesions in varied locations throughout the body. Samples were collected for subsequent cytopathology, fungal culture, serology, and molecular genotyping. Itraconazole was prescribed for the treatment of sporotrichosis and the animal was considered clinically cured at the end of 5 months of treatment and discharged. S. brasiliensis cat interactions can manifest with a multitude of clinical forms that resemble either infectious or noninfectious diseases. Both the need for meticulous cat physical evaluation by a veterinarian followed by accurate laboratory diagnosis are key Public Health measures in the Brazilian sporotrichosis hyperendemic area.
{"title":"Ocular lesions in a domestic feline:","authors":"G. Mothé, N. F. Reis, C. S. I. Melivilu, Aguinaldo Francisco Mendes Junior, Cinthia S Dos Santos, A. Dieckmann, R. L. Machado, Elisabeth M. S. Rocha, A. Baptista","doi":"10.11606/ISSN.1678-4456.BJVRAS.2021.183219","DOIUrl":"https://doi.org/10.11606/ISSN.1678-4456.BJVRAS.2021.183219","url":null,"abstract":"Sporotrichosis is a dermatozoonosis, caused by dimorphic pathogenic fungi of the genus Sporothrix. Although Sporothrix brasiliensis is the most frequent and pathogenic species identified from the Brazilian sporotrichosis hyperendemic, to the best of our knowledge this is the first report of its molecular diagnosis from a cat with ocular lesions. A 3-month old female, domestic feline presented an ocular manifestation with granuloma in the lower-left palpebral conjunctiva, in addition to mucocutaneous lesions in varied locations throughout the body. Samples were collected for subsequent cytopathology, fungal culture, serology, and molecular genotyping. Itraconazole was prescribed for the treatment of sporotrichosis and the animal was considered clinically cured at the end of 5 months of treatment and discharged. S. brasiliensis cat interactions can manifest with a multitude of clinical forms that resemble either infectious or noninfectious diseases. Both the need for meticulous cat physical evaluation by a veterinarian followed by accurate laboratory diagnosis are key Public Health measures in the Brazilian sporotrichosis hyperendemic area.","PeriodicalId":9119,"journal":{"name":"Brazilian Journal of Veterinary Research and Animal Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48652537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-07DOI: 10.11606/ISSN.1678-4456.BJVRAS.2021.180388
A. Dourado, I. S. Cordero, Anabela Filipa Rodrigues Gomes, Luís Lobo, Maria Isabel Ribeiro Dias
A clinical case in which skin and hair color change occurred after sacrococcygeal epidural anesthesia in a nine-month-old Siamese cross queen undergoing ovariohysterectomy (OHE) is described. Six weeks after surgery, during a re-check, it was noted that in the sacrococcygeal region the color of the skin and new hair growth was dark with a color comparable to the color present on the body extremities (muzzle, pinnae, legs, and tail). The skin and new hair growth of the shaved abdomen presented a standard color. The key enzyme of the melanogenic pathway in mammals is tyrosinase (TYR), and the Siamese temperature-sensitive phenotype is the result of genetic mutations that makes TYR function thermolabile. The activity of TYR in these cats is limited to the extremities where the temperature is lower while pigment production is impaired in the other body areas. The trichotomy of the sacrococcygeal region performed during wintertime in an outdoor cat was probably the trigger for increased activity of TYR in this area promoting pigment production. The absence of the same alterations in the abdominal area may be justified by less exposure of that region to the external environment, as well as to the feline habits of sedentarism, that avoid significant cooling in these regions. This report highlights the importance of taking this type of occurrence into account when performing an epidural in the Siamese cat breed. Also, to avoid skin color change in this breed, the authors recommend a midline abdominal instead of a flank approach to perform OHE.
{"title":"Transient skin and fur color change after trichotomy of the sacrococcygeal region for epidural anesthesia in a domestic cat (Felis catus)","authors":"A. Dourado, I. S. Cordero, Anabela Filipa Rodrigues Gomes, Luís Lobo, Maria Isabel Ribeiro Dias","doi":"10.11606/ISSN.1678-4456.BJVRAS.2021.180388","DOIUrl":"https://doi.org/10.11606/ISSN.1678-4456.BJVRAS.2021.180388","url":null,"abstract":"A clinical case in which skin and hair color change occurred after sacrococcygeal epidural anesthesia in a nine-month-old Siamese cross queen undergoing ovariohysterectomy (OHE) is described. Six weeks after surgery, during a re-check, it was noted that in the sacrococcygeal region the color of the skin and new hair growth was dark with a color comparable to the color present on the body extremities (muzzle, pinnae, legs, and tail). The skin and new hair growth of the shaved abdomen presented a standard color. The key enzyme of the melanogenic pathway in mammals is tyrosinase (TYR), and the Siamese temperature-sensitive phenotype is the result of genetic mutations that makes TYR function thermolabile. The activity of TYR in these cats is limited to the extremities where the temperature is lower while pigment production is impaired in the other body areas. The trichotomy of the sacrococcygeal region performed during wintertime in an outdoor cat was probably the trigger for increased activity of TYR in this area promoting pigment production. The absence of the same alterations in the abdominal area may be justified by less exposure of that region to the external environment, as well as to the feline habits of sedentarism, that avoid significant cooling in these regions. This report highlights the importance of taking this type of occurrence into account when performing an epidural in the Siamese cat breed. Also, to avoid skin color change in this breed, the authors recommend a midline abdominal instead of a flank approach to perform OHE.","PeriodicalId":9119,"journal":{"name":"Brazilian Journal of Veterinary Research and Animal Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49416111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-06DOI: 10.11606/issn.1678-4456.bjvras.2021.186535
A. J. Zanella, Mariana Bombo Perozzi Gameiro
Editorial for the special Donkeys and Mules
《驴和骡子》特辑社论
{"title":"Editorial","authors":"A. J. Zanella, Mariana Bombo Perozzi Gameiro","doi":"10.11606/issn.1678-4456.bjvras.2021.186535","DOIUrl":"https://doi.org/10.11606/issn.1678-4456.bjvras.2021.186535","url":null,"abstract":"Editorial for the special Donkeys and Mules","PeriodicalId":9119,"journal":{"name":"Brazilian Journal of Veterinary Research and Animal Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44120934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-28DOI: 10.11606/issn.1678-4456.bjvras.2021.174325
J. Rodrigues, Z. Raw, E. Santurtun, Fiona Cooke, Cara L. Clancy
Donkeys have a long history in the development of human societies. Typically referred to as a beast of burden, traditional uses for donkeys have included the transportation of goods and people, use in agricultural and forestry activities, to access water, and provide citizens in low- and middle-income countries a means of making an income for communities. However, the rise of mechanization, the development of modern farming techniques, and the increasing availability of motorized vehicles have led to donkeys and mules becoming redundant from traditional roles in many parts of the world. We provide examples of where donkeys have successfully transitioned from traditional roles to new, non-traditional roles in Europe and North America, and demonstrate that, although the roles and use of donkeys and mules are changing in a rapidly developing world, we can learn lessons from the past and apply them to current challenges. As the need for working equids declines in transport and agriculture, they still hold great value for recreational, therapeutic, and environmentally friendly methods of animal traction.
{"title":"Donkeys in transition","authors":"J. Rodrigues, Z. Raw, E. Santurtun, Fiona Cooke, Cara L. Clancy","doi":"10.11606/issn.1678-4456.bjvras.2021.174325","DOIUrl":"https://doi.org/10.11606/issn.1678-4456.bjvras.2021.174325","url":null,"abstract":"Donkeys have a long history in the development of human societies. Typically referred to as a beast of burden, traditional uses for donkeys have included the transportation of goods and people, use in agricultural and forestry activities, to access water, and provide citizens in low- and middle-income countries a means of making an income for communities. However, the rise of mechanization, the development of modern farming techniques, and the increasing availability of motorized vehicles have led to donkeys and mules becoming redundant from traditional roles in many parts of the world. We provide examples of where donkeys have successfully transitioned from traditional roles to new, non-traditional roles in Europe and North America, and demonstrate that, although the roles and use of donkeys and mules are changing in a rapidly developing world, we can learn lessons from the past and apply them to current challenges. As the need for working equids declines in transport and agriculture, they still hold great value for recreational, therapeutic, and environmentally friendly methods of animal traction. ","PeriodicalId":9119,"journal":{"name":"Brazilian Journal of Veterinary Research and Animal Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44350971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-23DOI: 10.11606/issn.1678-4456.bjvras.2021.178032
A. Berghiche, Chahinése Djebrane, N. Belahcene, N. Boulebda
Currently, the use of alternative medicine is necessary, even in animals. Our study focused on the valorisation of the use of herbal products in Algerian veterinary medicine. This study is based on a 6-month exploratory survey of 257 private practicing veterinarians, followed by a study of the bacteriological activity of the essential oil of the Pistacia lentiscus plant. The survey results generally indicate that private veterinarians support phytotherapy as an alternative to conventional drugs in several areas and species. Also, the results of research conducted on the antibacterial activity of Pistacia lentiscus essential oil show that it inactivates 50% of the bacterial strains. This confirms the well defined role of this medicinal plant used as an alternative in veterinary medicine.
{"title":"Veterinary phytotherapy in Algeria","authors":"A. Berghiche, Chahinése Djebrane, N. Belahcene, N. Boulebda","doi":"10.11606/issn.1678-4456.bjvras.2021.178032","DOIUrl":"https://doi.org/10.11606/issn.1678-4456.bjvras.2021.178032","url":null,"abstract":"Currently, the use of alternative medicine is necessary, even in animals. Our study focused on the valorisation of the use of herbal products in Algerian veterinary medicine. This study is based on a 6-month exploratory survey of 257 private practicing veterinarians, followed by a study of the bacteriological activity of the essential oil of the Pistacia lentiscus plant. The survey results generally indicate that private veterinarians support phytotherapy as an alternative to conventional drugs in several areas and species. Also, the results of research conducted on the antibacterial activity of Pistacia lentiscus essential oil show that it inactivates 50% of the bacterial strains. This confirms the well defined role of this medicinal plant used as an alternative in veterinary medicine.","PeriodicalId":9119,"journal":{"name":"Brazilian Journal of Veterinary Research and Animal Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45476685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-07DOI: 10.11606/ISSN.1678-4456.BJVRAS.2021.179885
N. F. Paretsis, Vagner Gonçalves Junior, Nicolle Queiroz Hazarbassanov, G. M. Marcondes, A. M. G. Plepis, V. Martins, V. Arana-Chavez, J. Fülber, A. L. D. De Zoppa
Hydroxyapatite, chitosan, and carbon nanotube composite biomaterial were developed to improve bone healing. Previous studies suggested that a combination of biomaterials and mesenchymal stem cells (MSCs) can potentially help promote bone regeneration. In the present study, we first developed hydroxyapatite, chitosan, and carbon nanotube composite biomaterial. Then, the effect of different concentrations of the extract on the viability of Vero cells (ATCC CCL-81) and MSCs obtained from sheep bone marrow using methylthiazol tetrazolium (MTT) and propidium iodide (PI) assays were evaluated. The biomaterial group demonstrated an absence of cytotoxicity, similar to the control group. Samples with 50% and 10% biomaterial extract concentrations showed higher cell viability compared to samples from the control group (MTT assay). These results suggest that the presence of this composite biomaterial can be used with MSCs. This study also concluded that hydroxyapatite, chitosan, and carbon nanotube composite biomaterial were not cytotoxic. Therefore, these could be used for performing in vivo tests.
{"title":"Avaliação in vitro do biomaterial compósito à base de hidroxiapatita, quitosana e nanotubo de carbono como adjuvante no reparo ósseo","authors":"N. F. Paretsis, Vagner Gonçalves Junior, Nicolle Queiroz Hazarbassanov, G. M. Marcondes, A. M. G. Plepis, V. Martins, V. Arana-Chavez, J. Fülber, A. L. D. De Zoppa","doi":"10.11606/ISSN.1678-4456.BJVRAS.2021.179885","DOIUrl":"https://doi.org/10.11606/ISSN.1678-4456.BJVRAS.2021.179885","url":null,"abstract":"Hydroxyapatite, chitosan, and carbon nanotube composite biomaterial were developed to improve bone healing. Previous studies suggested that a combination of biomaterials and mesenchymal stem cells (MSCs) can potentially help promote bone regeneration. In the present study, we first developed hydroxyapatite, chitosan, and carbon nanotube composite biomaterial. Then, the effect of different concentrations of the extract on the viability of Vero cells (ATCC CCL-81) and MSCs obtained from sheep bone marrow using methylthiazol tetrazolium (MTT) and propidium iodide (PI) assays were evaluated. The biomaterial group demonstrated an absence of cytotoxicity, similar to the control group. Samples with 50% and 10% biomaterial extract concentrations showed higher cell viability compared to samples from the control group (MTT assay). These results suggest that the presence of this composite biomaterial can be used with MSCs. This study also concluded that hydroxyapatite, chitosan, and carbon nanotube composite biomaterial were not cytotoxic. Therefore, these could be used for performing in vivo tests.","PeriodicalId":9119,"journal":{"name":"Brazilian Journal of Veterinary Research and Animal Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43103073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-03DOI: 10.11606/ISSN.1678-4456.BJVRAS.2021.178109
G. M. Costa, Núbia Aparecida Ribeiro, M. Gonçalves, J. Silva, D. Custódio, G. F. Mian
Bovine mastitis is the most important disease of dairy herds worldwide. Its main etiologic agents are bacteria, including Streptococcus agalactiae. The importance of this agent in bovine mastitis is because it is highly contagious and has a high impact on the occurrence of clinical mastitis cases and in the increase of the bulk milk somatic cell counts. The dry cow therapy and the treatment of the clinical mastitis cases stand out among the measures to control intramammary infections in cows. However, these strategies require knowledge about the antimicrobial susceptibility of the causal microorganisms. Thus, this study aimed to evaluate the antimicrobial susceptibility of 89 S. agalactiae strains isolated from bovine mastitis between the years 2004 and 2008 in dairy herds from Campo das Vertentes region, Minas Gerais State, Brazil. The disc diffusion technique was used and the antimicrobials currently used in mastitis therapy were tested. The isolates tested showed 100% susceptibility to chloramphenicol, ceftiofur, cefotaxime, enrofloxacin, and cefquinome. High frequencies of susceptibility (>95%) were also observed for the beta-lactams (penicillin G, ampicillin, and oxacillin), cephalosporins (cephalotin, ceftiofur, cefotaxime, cefoperazone, and cefquinome), florfenicol, gentamicin, lincomycin, nitrofurantoin, and sulfamethoprim. The strains showed high frequencies of resistance to neomycin (15.74%), and tetracycline (21.35%). Multidrug resistance was detected in 2.25% of the tested isolates. The results pointed to variations in the antimicrobial susceptibility profiles of the studied strains and the importance of the use of the susceptibility tests to determine the correct antimicrobial to be applied in the treatment of bovine mastitis caused by S. agalactiae. The high frequencies of resistance observed to some antimicrobials, such as neomycin and tetracycline, commonly used in the treatment of mastitis and other pathologies, highlighted the need for more judicious use of antimicrobials on dairy farms.
乳腺炎是世界范围内奶牛群中最重要的疾病。它的主要病原体是细菌，包括无乳链球菌。这种制剂在牛乳腺炎中的重要性在于，它具有高度传染性，对临床乳腺炎病例的发生和散装乳体细胞计数的增加有很大影响。干牛疗法和临床乳腺炎病例的治疗是控制奶牛乳内感染的重要措施。然而，这些策略需要了解致病微生物的抗菌易感性。因此，本研究旨在评估2004年至2008年间从巴西米纳斯吉拉斯州Campo das Vertentes地区的奶牛群中分离的89株无乳链球菌的抗菌敏感性。采用椎间盘扩散技术，并对目前用于乳腺炎治疗的抗菌药物进行了测试。测试的分离株对氯霉素、头孢噻呋、头孢噻肟、恩诺沙星和头孢喹酮的敏感性为100%。β-内酰胺类药物（青霉素G、氨苄青霉素和苯唑西林）、头孢菌素类药物（头孢噻呋、头孢噻肟、头孢哌酮和头孢喹酮）、氟苯尼考、庆大霉素、林可霉素、呋喃妥因和磺胺甲脒的易感性也很高（>95%）。菌株对新霉素和四环素的耐药率分别为15.74%和21.35%，2.25%的菌株对多种药物产生耐药性。研究结果表明，所研究菌株的抗微生物药敏特性存在差异，使用药敏试验来确定治疗无乳链球菌引起的牛乳腺炎的正确抗菌药物的重要性。对一些常用于治疗乳腺炎和其他疾病的抗菌药物，如新霉素和四环素，观察到的耐药性频率很高，这突出表明在奶牛场更明智地使用抗菌药物的必要性。
{"title":"Antimicrobial susceptibility profile of Streptococcus agalactiae strains isolated from bovine mastitis","authors":"G. M. Costa, Núbia Aparecida Ribeiro, M. Gonçalves, J. Silva, D. Custódio, G. F. Mian","doi":"10.11606/ISSN.1678-4456.BJVRAS.2021.178109","DOIUrl":"https://doi.org/10.11606/ISSN.1678-4456.BJVRAS.2021.178109","url":null,"abstract":"Bovine mastitis is the most important disease of dairy herds worldwide. Its main etiologic agents are bacteria, including Streptococcus agalactiae. The importance of this agent in bovine mastitis is because it is highly contagious and has a high impact on the occurrence of clinical mastitis cases and in the increase of the bulk milk somatic cell counts. The dry cow therapy and the treatment of the clinical mastitis cases stand out among the measures to control intramammary infections in cows. However, these strategies require knowledge about the antimicrobial susceptibility of the causal microorganisms. Thus, this study aimed to evaluate the antimicrobial susceptibility of 89 S. agalactiae strains isolated from bovine mastitis between the years 2004 and 2008 in dairy herds from Campo das Vertentes region, Minas Gerais State, Brazil. The disc diffusion technique was used and the antimicrobials currently used in mastitis therapy were tested. The isolates tested showed 100% susceptibility to chloramphenicol, ceftiofur, cefotaxime, enrofloxacin, and cefquinome. High frequencies of susceptibility (>95%) were also observed for the beta-lactams (penicillin G, ampicillin, and oxacillin), cephalosporins (cephalotin, ceftiofur, cefotaxime, cefoperazone, and cefquinome), florfenicol, gentamicin, lincomycin, nitrofurantoin, and sulfamethoprim. The strains showed high frequencies of resistance to neomycin (15.74%), and tetracycline (21.35%). Multidrug resistance was detected in 2.25% of the tested isolates. The results pointed to variations in the antimicrobial susceptibility profiles of the studied strains and the importance of the use of the susceptibility tests to determine the correct antimicrobial to be applied in the treatment of bovine mastitis caused by S. agalactiae. The high frequencies of resistance observed to some antimicrobials, such as neomycin and tetracycline, commonly used in the treatment of mastitis and other pathologies, highlighted the need for more judicious use of antimicrobials on dairy farms.","PeriodicalId":9119,"journal":{"name":"Brazilian Journal of Veterinary Research and Animal Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43309528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-03DOI: 10.11606/ISSN.1678-4456.BJVRAS.2021.177172
Nazan Baksi, A. Simsek
O calicivirus felino esta entre os microrganismos patogenicos mais comuns nas doencas do trato respiratorio superior de gatos, determinando estomatites, ulceracoes orais, descarga ocular e nasal, conjuntivite, febre, manqueira, anorexia, hipersalivacao, pneumonia, disturbios respiratorios, tosse e depressao. O presente trabalho foi delineado para determinar o papel do calicivirus felino (CVF) em gatos com doencas do trato respiratorio superior na regiao de Diyarbakir, Turquia. Com o objetivo de orientar a prescricao do tratamento para os gatos infectados e contribuir com a profilaxia da doenca. O material de estudo consistiu em 10 gatos saudaveis sem qualquer problema de saude e 20 gatos acometidos por doencas do trato respiratorio superior que nao haviam sido vacinados contra a infeccao pelo calicivirus felino. Os animais de diferentes racas, idades e generos foram encaminhados para a Universidade de Dicle, na Faculdade de Veterinaria, na policlinica Professor Dr. Servet Sekin. Apos o exame clinico de rotina dos animais, foram colhidos swabs orais e da conjuntiva e amostras de sangue. Analises hematologicas e bioquimicas das amostras de sangue foram realizadas e os swabs foram analisados pelo metodo da reacao em cadeia pela polimerase (PCR) para diagnostico do agente. Nos gatos infectados foram constatadas: lesoes orais, hipersalivacao, descargas oculares e nasais, tosse e dificuldade respiratoria. O calicivirus felino foi detectado pela tecnica de PCR no swab conjuntival de apenas um gato. A conclusao obtida foi que a infeccao pelo calicivirus felino foi detectada pela tecnica de PCR na regiao de Diyarbakir, Turquia, em gatos com doenca do trato respiratorio superior com a frequencia de 5%.
{"title":"Investigation of Feline calicivirus infection in cats with upper respiratory tract disease in Diyarbakir, Turkey","authors":"Nazan Baksi, A. Simsek","doi":"10.11606/ISSN.1678-4456.BJVRAS.2021.177172","DOIUrl":"https://doi.org/10.11606/ISSN.1678-4456.BJVRAS.2021.177172","url":null,"abstract":"O calicivirus felino esta entre os microrganismos patogenicos mais comuns nas doencas do trato respiratorio superior de gatos, determinando estomatites, ulceracoes orais, descarga ocular e nasal, conjuntivite, febre, manqueira, anorexia, hipersalivacao, pneumonia, disturbios respiratorios, tosse e depressao. O presente trabalho foi delineado para determinar o papel do calicivirus felino (CVF) em gatos com doencas do trato respiratorio superior na regiao de Diyarbakir, Turquia. Com o objetivo de orientar a prescricao do tratamento para os gatos infectados e contribuir com a profilaxia da doenca. O material de estudo consistiu em 10 gatos saudaveis sem qualquer problema de saude e 20 gatos acometidos por doencas do trato respiratorio superior que nao haviam sido vacinados contra a infeccao pelo calicivirus felino. Os animais de diferentes racas, idades e generos foram encaminhados para a Universidade de Dicle, na Faculdade de Veterinaria, na policlinica Professor Dr. Servet Sekin. Apos o exame clinico de rotina dos animais, foram colhidos swabs orais e da conjuntiva e amostras de sangue. Analises hematologicas e bioquimicas das amostras de sangue foram realizadas e os swabs foram analisados pelo metodo da reacao em cadeia pela polimerase (PCR) para diagnostico do agente. Nos gatos infectados foram constatadas: lesoes orais, hipersalivacao, descargas oculares e nasais, tosse e dificuldade respiratoria. O calicivirus felino foi detectado pela tecnica de PCR no swab conjuntival de apenas um gato. A conclusao obtida foi que a infeccao pelo calicivirus felino foi detectada pela tecnica de PCR na regiao de Diyarbakir, Turquia, em gatos com doenca do trato respiratorio superior com a frequencia de 5%.","PeriodicalId":9119,"journal":{"name":"Brazilian Journal of Veterinary Research and Animal Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45752209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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