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Molecular and Morphological Variation among Populations ofPediomelum tenuiflorum (Pursh) A.N. Egan (Fabaceae) inNebraska, USA 美国内布拉斯加州豆科小檗(pediomelum tenuflorum, Pursh) A.N. Egan)居群的分子和形态变异
Pub Date : 2019-07-31 DOI: 10.32873/UNL.DC.TNAS.39.17
C. Kellar, P. R. Kellar
Individuals of Pediomelum tenuiflorum, “wild alfalfa”, from disjunct populations in Nebraska vary extensively in their overall gestalt.Those in the western and central part of the state have a very slender growth habit, with thin stems and few, small flowers; whereas,those in the southeast have a very robust growth habit with heavy-looking stems and many tightly clustered flowers. For nearly 200years, taxonomists have alternated between splitting P. tenuiflorum into two species, with the many-flowered morphotype namedP. floribundum, and lumping all the morphological variants into one species as they are now. In this study, we investigated morphologicaland molecular characters that could be used to clarify taxonomic classifications of these morphotypes. We measured 10morphological characters on 51 specimens and sequenced nearly 300,000 nucleotide characters on the Illumina platform from threecellular genomes in seven samples of Pediomelum plus an outgroup taxon. Results revealed six significantly different morphologicalcharacters but ambiguous evolutionary histories of the plastid and mitochondrial genomes in P. tenuiflorum. Our complete plastidgenomes and genes and noncoding regions of the mitochondrial genome may be used as a foundation for studying the evolutionaryhistories of these genomes. Additionally, we identified seven highly variable genomic regions in the chloroplast genome uponwhich a molecular phylogenetic investigation on an expanded set of samples from across the species’ geographic distribution canbe conducted to further define the taxonomic placements of P. tenuiflorum and P. floribundum.
来自内布拉斯加州间断种群的细花Pediomelum“野生苜蓿”的个体在整体格式塔上差异很大。该州西部和中部的植物生长习性非常纤细,茎细,花朵很少;而东南部的植物则有着非常健壮的生长习惯,茎干粗壮,花朵紧密簇生。近200年来,分类学家一直在交替地将P.teniflorum分为两个物种,多花形态型命名为dP。floribundum,并将所有形态变异集中到一个物种中。在本研究中,我们研究了形态和分子特征,这些特征可用于阐明这些形态类型的分类。我们测量了51个标本的10个形态特征,并在Illumina平台上对7个Pediomelum样本和一个外群分类单元的3个细胞基因组中的近300000个核苷酸特征进行了测序。结果表明,细花P.teniflorum的质体和线粒体基因组有六个明显不同的形态特征,但进化史不明确。我们完整的质体单体、基因和线粒体基因组的非编码区可以作为研究这些基因组进化史的基础。此外,我们在叶绿体基因组中确定了七个高度可变的基因组区域,在这些区域上,可以对来自不同物种地理分布的一组扩展样本进行分子系统发育研究,以进一步确定细花P.teniflorum和花P.floribundum的分类位置。
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引用次数: 0
Status of the Plains Harvest Mouse (Reithrodontomys montanus griseus) in eastern Nebraska 内布拉斯加州东部平原收割鼠(Reithrodontomys montanus griseus)现状
Pub Date : 2019-03-27 DOI: 10.32873/unl.dc.tnas.39.10
Keith Geluso, Greg D. Wright
In eastern Nebraska, current status of the Plains Harvest Mouse (Reithrodontomys montanus griseus) is not well understood. Infrequent captures during the last century have led to a paucity of information regarding this taxon, and some researchers postulate that its distribution has contracted in the state. In 2008, we conducted a field survey for R. m. griseus in eastern Nebraska, amassed prior specimen records, and examined most of the specimens for this subspecies from the state to better understand its distribution, natural history, and subspecific status. In our field efforts, we only captured a single individual despite > 8,000 trap nights in suitable habitats. Our literature review and queries for vouchers yielded 20 specimens of R. m. griseus from eastern Nebraska, based on published distributional limits for this subspecies. In eastern Nebraska, R. m. griseus has been captured in tallgrass prairies, short-grass upland pastures, roadside ditches, and open areas associated with salt flats. Observations across eastern Nebraska in the last 40 years demonstrate that this taxon still exists across the entire region and has been captured more frequently in cooler months. We suspect that some combination of low abundance, trap shyness, and trapping biases towards heavily vegetated habitats and warm seasons likely has led to infrequent captures of R. m. griseus in eastern Nebraska. After examination of many museum specimens of this species from across Nebraska, Kansas, and Missouri, we questioned the delineations in distribution for the two subspecies in the region. On the basis of dorsal gray fur coloration, R. m. albescens appears limited to the Sandhill and Panhandle regions of Nebraska, whereas all of eastern and southern Nebraska as well as Kansas and western Missouri represent R. m. griseus, a subspecies with brown dorsal fur coloration. Based on our proposed distributional changes for these two subspecies in Nebraska, we do not find that either subspecies requires conservation efforts. Reithrodontomys montanus griseus likely will persist at low densities throughout eastern Nebraska in appropriate habitats and persist at higher densities farther westward in southern parts of the state. Reithrodontomys montanus albescens always has been more common in the Sandhill Region of Nebraska, as individuals still are observed and captured with regularity.
在内布拉斯加州东部,平原收获鼠(Reithrodontomys montanus griseus)的现状尚不清楚。上个世纪的罕见捕获导致了有关该分类单元的信息匮乏,一些研究人员推测其在该州的分布已经缩小。2008年,我们在内布拉斯加州东部对灰尾蛇进行了一次实地调查,收集了之前的标本记录,并检查了该州该亚种的大部分标本,以更好地了解其分布、自然历史和亚种状况。在我们的野外工作中,尽管在合适的栖息地有8000多个陷阱之夜,但我们只捕获了一个个体。根据已公布的该亚种的分布限制,我们的文献综述和对凭证的查询从内布拉斯加州东部获得了20个灰色R.m.griseus标本。在内布拉斯加州东部,R.m.griseus在高草草原、短草高地牧场、路边沟渠和与盐滩相关的开阔地带被捕获。过去40年在内布拉斯加州东部的观测表明,该分类单元仍然存在于整个地区,并且在较冷的月份被更频繁地捕获。我们怀疑,低丰度、陷阱害羞、对植被茂密的栖息地和温暖季节的陷阱偏见等因素的结合,可能导致在内布拉斯加州东部很少捕捉到灰蝶。在检查了来自内布拉斯加州、堪萨斯州和密苏里州的许多该物种的博物馆标本后,我们对该地区这两个亚种的分布情况提出了质疑。根据背部灰色皮毛的颜色,R.m.albescens似乎仅限于内布拉斯加州的Sandhill和Panhandle地区,而内布拉斯加州东部和南部以及堪萨斯州和密苏里州西部的R.m.griseus是一个背部棕色皮毛的亚种。根据我们提出的这两个亚种在内布拉斯加州的分布变化,我们没有发现任何一个亚种需要保护。灰脊灰尾蠊可能会在内布拉斯加州东部的适当栖息地以低密度持续存在,并在该州南部以更高密度持续存在。在内布拉斯加的桑德希尔地区,由于仍有规律地观察和捕捉到个体,因此山地反照雷throdontomys一直更为常见。
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引用次数: 0
Population Characteristics of Co-Managed White Bass and Hybrid Striped Bass in Lake McConaughy, Nebraska 内布拉斯加州麦康纳湖白鲈鱼和杂交条纹鲈鱼的种群特征
Pub Date : 2019-01-01 DOI: 10.32873/unl.dc.tnas.39.1
B. J. Schall, C. Schoenebeck, K. Koupal
White Bass (Morone chrysops) and Hybrid Striped Bass (M. saxatillis × M. chrysops) populations often coexist in the same waterbody and are known to achieve different lengths, have differing reproductive success, and provide different opportunities for anglers. However, comparative population dynamics from systems where Moronids are managed with the same regulation is often lacking. This study aimed to assess the recruitment, mortality, and growth of these species from seasonal samples collected at Lake McConaughy in 2015 and fall 2016. White Bass demonstrated highly variable recruitment in Lake McConaughy despite stocking efforts (mean recruitment variability index = 0.157). Hybrid Striped Bass year class strength was also variable, but RVI was not calculated as the number of missing year classes exceed the number of present year classes. Total annual mortality estimates for Hybrid Striped Bass (39.7%) and White Bass (41.4%) were consistent with values reported in other populations, but both species were observed to achieve uncommon longevity. Both species exhibited sexual size dimorphism, and growth was moderate and consistent with average values presented for North American populations. This study provides insight into the population dynamics of two species with trophy potential managed by the same regulation in Lake McConaughy. Differences in growth patterns indicate the current regulation affects each species differently, and managers may be able to utilize this to inform decisions about management of coexisting Moronid populations.
白鲈鱼(Morone chrysops)和杂交条纹鲈鱼(M. saxatillis × M. chrysops)经常在同一水域共存,已知它们的长度不同,繁殖成功率不同,为垂钓者提供了不同的机会。然而,往往缺乏来自Moronids以相同规则管理的系统的比较人口动态。本研究旨在评估2015年和2016年秋季在麦康纳湖收集的季节性样本中这些物种的招募、死亡率和生长情况。尽管放养努力,白鲈鱼在McConaughy湖的捕捞表现出高度可变(平均捕捞变异性指数= 0.157)。杂交条纹鲈鱼的班级强度也有变化,但由于缺失班级的数量超过了当前班级的数量,因此没有计算RVI。杂交条纹鲈鱼(39.7%)和白鲈鱼(41.4%)的年总死亡率与其他种群的报告值一致,但观察到这两个物种的寿命都不寻常。这两个物种都表现出性别大小的二态性,生长适中,与北美种群的平均值一致。本研究对麦康纳湖两种具有狩猎潜力的物种的种群动态进行了深入研究。生长模式的差异表明当前的法规对每个物种的影响不同,管理者可以利用这一点来决定如何管理共存的Moronid种群。
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引用次数: 0
Spatial analysis of borrow pits along the Platte River in south-central Nebraska, USA, in 1957 and 2016 1957年和2016年美国内布拉斯加州中南部普拉特河沿岸取土坑的空间分析
N. Pauley, M. Harner, E. Buckley, P. Burger, Keith Geluso
: The Central Platte River Valley (CPRV) of Nebraska provides critical habitat for wildlife, while serving agricultural, indus trial, and other human uses. Mining of sand and gravel from the floodplain of the Platte River has supported construction of roads and other uses, and this extraction has created many borrow-pit ponds, lakes, and other small bodies of standing water (hereafter borrow-pits), further transforming riparian and prairie habitats. The objective of this study was to compare the abundance, size, and distribution of borrow pits before construction of Interstate 80 (1957) and at present (2016) from Lexington to Chapman, Nebraska, a length of river spanning about 146 km (90 mi) and sometimes referred to as the Big Bend Reach. Orthorectified aerial imagery of the Platte River was obtained for years 1957 and 2016, and we digitized the standing bodies of water within the floodplain in Arc -GIS. Total numbers of borrow pits and measures of pit shape were calculated and compared between floodplain regions where im agery overlapped. From 1957 to 2016, the number of borrow pits increased from 300 to 786, total area occupied by pits expanded by 538%, and total shoreline of pits increased by 261%. In 2016, aerial imagery was available for a larger extent of the floodplain and contained a total of 1,062 borrow pits covering 16 km 2 (6 mi 2 ), with a total shoreline of 581 km (361 mi). For context, the Platte River channels’ approximate area was 45 km 2 (17 mi 2 ) and shoreline 1,582 km (983 mi) between Lexington and Chapman, Nebraska, in 2016. Results provide insight into historical and current presence, distribution, and shape of borrow pits along the Platte River, as well as serve as a reference point for future studies investigating regional landscape change and ecological effects of creating hun dreds of borrow pits on the floodplain.
:内布拉斯加州的中央普拉特河谷(CPRV)为野生动物提供了重要的栖息地,同时为农业、工业和其他人类用途服务。从普拉特河泛滥平原开采沙子和砾石支持了道路建设和其他用途,这种开采创造了许多取土坑池塘、湖泊和其他小型积水(以下简称取土坑),进一步改变了河岸和草原栖息地。本研究的目的是比较80号州际公路建设之前(1957年)和现在(2016年)从列克星敦到内布拉斯加州查普曼的取土坑的丰度、大小和分布,这条河长约146公里(90英里),有时被称为大弯河段。普拉特河的正射校正航空图像是在1957年和2016年获得的,我们在Arc-GIS中对泛滥平原内的直立水体进行了数字化。计算并比较了河流重叠的洪泛平原地区的取土坑总数和坑形测量。从1957年到2016年,取土坑的数量从300个增加到786个,取土坑占用的总面积扩大了538%,取土坑总海岸线增加了261%。2016年,航空图像可用于更大范围的泛滥平原,共包含1062个取土坑,占地16平方公里(6英里),总海岸线为581公里(361英里)。就上下文而言,2016年,位于内布拉斯加州列克星敦和查普曼之间的普拉特河河道的大致面积为45平方公里(17英里),海岸线为1582公里(983英里)。研究结果深入了解了普拉特河沿岸取土坑的历史和现状、分布和形状,并为未来研究区域景观变化和在泛滥平原上建造大量取土坑的生态影响提供了参考。
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引用次数: 4
Small mammals in cornfields and associated peripheral habitats in central Nebraska 内布拉斯加州中部玉米地及周边栖息地的小型哺乳动物
Tyson J. Spanel, Keith Geluso
In the Great Plains, many native grasslands have been converted to agricultural fields during the last two centuries. Peripheral habi tats along edges of crop fields generally consist of linear habitats along roads, with many of these habitats used by native fauna. Our study examined capture rates and species composition of small mammals in cornfields, herbaceous roadside ditches, and wooded shelterbelts in central Nebraska. We captured nine species of small mammals. The Prairie Vole ( Microtus ochrogaster ) and Western Harvest Mouse ( Reithrodontomys megalotis ) were captured almost exclusively in roadside ditches, the White-footed Deermouse ( Peromyscus leucopus ) was captured most often in wooded shelterbelts, and the North American Deermouse ( Peromyscus maniculatus ) was common to abundant in all three habitats. Capture rates of small mammals were greatest in roadside ditches and least in cornfields. Herbaceous roadside ditches contained the greatest species richness with seven species, whereas shelterbelts and corn fields each had five species. Species composition of rodents differed in shelterbelts comprised solely of eastern red-cedars ( Juniperus virginiana ) compared to those with a mixture deciduous and coniferous trees. Our study demonstrated that roadside ditches asso ciated with agricultural fields serve as habitats for many prairie species whereas wooded shelterbelts along agricultural fields sup port both woodland and prairie species in central Nebraska. Cornfields mainly were inhabited by North American Deermice but did not support many other species of small mammals. Although agricultural practices have reduced the quantity of grasslands for prairie species across the region, habitats associated with periphery of fields appear to serve as alternative habitats for small mam mals throughout the Great Plains.
在过去的两个世纪里,大平原上的许多原生草原都变成了农田。农田边缘的外围栖息地通常由道路沿线的线性栖息地组成,其中许多栖息地由本地动物使用。我们的研究调查了内布拉斯加州中部玉米地、草本路边沟渠和树木繁茂的防护林中小型哺乳动物的捕获率和物种组成。我们捕获了九种小型哺乳动物。草原田鼠(Microtus ochragaster)和西部收获鼠(Reithrodontomys megalotis。小型哺乳动物的捕获率在路边沟渠中最高,在玉米地中最低。草本路边沟的物种丰富度最高,有7种,而防护林和玉米地各有5种。与落叶树和针叶树混合的防护林相比,仅由东部红雪松(Juniperus virginiana)组成的防护林中啮齿动物的物种组成不同。我们的研究表明,与农田相关的路边沟渠是许多草原物种的栖息地,而农田沿线的树木防护林则支持内布拉斯加州中部的林地和草原物种。康菲尔德主要居住着北美鹿,但不支持许多其他种类的小型哺乳动物。尽管农业实践减少了该地区草原物种的草原数量,但与田地周边相关的栖息地似乎是整个大平原小马头的替代栖息地。
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引用次数: 2
Can Lactate Dehydrogenase be used to Index Anaerobic Activity in Fishes? 乳酸脱氢酶能否作为鱼类厌氧活性指标?
C. Schoenebeck, Erik Prenosil, Brianna Pallas, A. Abrams, K. A. Carlson
Anaerobic activities such as those associated with spawning, foraging for prey, and predator avoidance are difficult to quantify in wild fish. This study experimentally evaluated if the muscle enzyme lactate dehydrogenase (LDH) can be used to index recent anaerobic activity in fish by testing the hypothesis that muscle LDH activity will be greater in exercised fish than in rested fish. We used burst swimming motions in a swim tunnel to elicit anaerobic metabolism in a 5 day anaerobic exercise treatment (n = 30) and a rested control group (n = 30). On average the exercised fish produced significantly more LDH in their muscle tissue (average = 19.5 IU/ug, SE = 1.8, F = 12.88, df = 2, 57, P < 0.001) due to the increased anaerobic activity than rested fish (average = 13.4 IU/ug, SE = 0.9). However, large individual variability in LDH activity within groups resulted in some overlap between treatment groups. Therefore we suggest limiting the use of LDH activity to infer relative comparisons of anaerobic activity among groups until the relationship is more clearly understood.
厌氧活动,如与产卵、觅食和躲避捕食者有关的活动,在野生鱼类中很难量化。本研究通过测试运动鱼类的肌肉LDH活性高于休息鱼类的假设,通过实验评估了肌肉酶LDH是否可以用于指示鱼类最近的厌氧活性。在为期5天的无氧运动治疗(n=30)和休息的对照组(n=30。平均而言,与休息鱼类(平均=13.4 IU/ug,SE=0.9)相比,由于无氧活性增加,运动鱼类的肌肉组织中产生的LDH显著增加(平均=19.5 IU/ug、SE=1.8、F=12.88、df=2、57,P<0.001)。然而,组内LDH活性的个体差异较大,导致治疗组之间存在一些重叠。因此,我们建议限制使用LDH活性来推断各组之间厌氧活性的相对比较,直到更清楚地了解这种关系。
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引用次数: 1
Sister Chromatid Exchanges in A Male With A Y/Y Translocation. 雌雄易位的雄性姐妹染色单体交换。
Merlin G Butler

Sister chromatid exchanges (SCE) are considered indicators of genetic damage and early chromosome changes. The SCE frequency from an infertile 27-year-old, white male with tall stature and a nondicentric Y/Y translocation consisting of one short arm and two long arms of the Y chromosome was determined. The SCE frequency was 7.9 ± SD 1.4 while the SCE frequency from ten control subjects was 8.4 ± SD 0.51. A two-tailed t-test was applied to the SCE data and no significant difference was found between the individual with the Y/Y translocation and control subjects. Apparently, this altered chromosome does not interfere with the total chromosome behavior and the number of SCEs produced when compared to ten control subjects of both sexes.

姐妹染色单体交换(SCE)被认为是遗传损伤和早期染色体改变的指标。测定了一名27岁的白人男性不育患者的SCE频率,该患者身高高,Y染色体有一条短臂和两条长臂的非双中心Y/Y易位。10例对照的SCE频率为8.4±SD 0.51,对照组的SCE频率为7.9±SD 1.4。对SCE数据进行双尾t检验,发现Y/Y易位个体与对照组之间无显著差异。显然,与10个两性对照对象相比,这条改变的染色体不会干扰染色体的总体行为和产生的sce数量。
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引用次数: 0
期刊
Transactions of the Nebraska Academy of Sciences and affiliated societies
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