A. Moghadamnia, M. Hashemi, Parvin Sajadi Kaboudi, Z. Geraili
Background and Aim. In traditional medicine, the use of Trachyspermum ammi, commonly known as 'Ajwain', is recommended to improve digestive function of the stomach and has effects on body weight. The present study aims to determine the effect different concentrations of mixed organic solvents extract (MOSE) of Trachyspermum ammi on mice compared with control group. Materials and Methods. This experimental-interventional study was performed on mice weighing an average of 21 gr, selected through random allocation method. The mice were divided into four groups of 24 (one control group and three case groups), receiving 0.5, 1 and 2% concentrations or density of MOSE of Trachyspermum ammi (0.001 mg/kg). First, the dried fruit of Trachyspermum ammi was powdered and then, it was extracted using various organic solvents including petroleum ether, chloroform, ethyl acetate and ethyl alcohol. The extract was fed to the mice for 14 days and their weight was controlled every day. Results. The hypothesis of diversity in the weight gain of mice among the four groups after 15 days was significant; weight gain in control group was significantly higher than other groups (p<0.05). In addition, comparing the weight gain of the mice that received different concentrations of MOSE of Trachyspermum ammi (0.5, 1 and 2%) did not show a significant difference (p<0.05). Conclusion. The use of Trachyspermum ammi plant can not be effect on body weight compared to the control group. This could not be a suitable way to weight change through diet.
{"title":"The Effect of Mixed Organic Solvents Extract of Trachyspermum ammi with Different Concentrations on the Body Weight of Mice","authors":"A. Moghadamnia, M. Hashemi, Parvin Sajadi Kaboudi, Z. Geraili","doi":"10.5539/JMBR.V9N1P49","DOIUrl":"https://doi.org/10.5539/JMBR.V9N1P49","url":null,"abstract":"Background and Aim. In traditional medicine, the use of Trachyspermum ammi, commonly known as 'Ajwain', is recommended to improve digestive function of the stomach and has effects on body weight. The present study aims to determine the effect different concentrations of mixed organic solvents extract (MOSE) of Trachyspermum ammi on mice compared with control group. \u0000 \u0000Materials and Methods. This experimental-interventional study was performed on mice weighing an average of 21 gr, selected through random allocation method. The mice were divided into four groups of 24 (one control group and three case groups), receiving 0.5, 1 and 2% concentrations or density of MOSE of Trachyspermum ammi (0.001 mg/kg). First, the dried fruit of Trachyspermum ammi was powdered and then, it was extracted using various organic solvents including petroleum ether, chloroform, ethyl acetate and ethyl alcohol. The extract was fed to the mice for 14 days and their weight was controlled every day. \u0000 \u0000Results. The hypothesis of diversity in the weight gain of mice among the four groups after 15 days was significant; weight gain in control group was significantly higher than other groups (p<0.05). In addition, comparing the weight gain of the mice that received different concentrations of MOSE of Trachyspermum ammi (0.5, 1 and 2%) did not show a significant difference (p<0.05). \u0000 \u0000Conclusion. The use of Trachyspermum ammi plant can not be effect on body weight compared to the control group. This could not be a suitable way to weight change through diet.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44099059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We evaluated biochemical analysis results with the aim of discovering serum levels that have possible effects and differences on age-related macular degeneration (AMD) and diabetic retinopathy (DRP). A retrospective case-control study was conducted between January 2017 and January 2018 on a total of 114 patients (84 DRP, 30 AMD) and 24 age and sex-matched control individuals. Four groups were created; 52 patients with proliferative DRP (PDR), 32 patients with nonproliferative PDR (non-PDR), 30 patients with wet AMD and 24 control individuals. Serum biochemical (HbA1C, fasting glucose, AST, ALT, C-reactive protein, albumin, total protein, uric acid, triglyceride, HDL, LDL, total cholesterol, Na, K, urine albumin) and complete blood count (CBC) analyses were performed at the time of diagnosis. Descriptive statistics, one-way ANOVA and Kruskal-Wallis tests were performed using SPSS to analyse data (Version 22.0). The mean age of patients was 63.3 years ± 6.4 (49-91year), and that of control individuals was 65.3 years ± 9.3 (50-88 year). Post hoc analysis showed statistically significant differences in HbA1c and fasting glucose levels among PDR-AMD, PDR-control, non-PDR-AMD, and non-PDR-control groups (whole, P
{"title":"Comparison of Serum Biochemical and Haematological Analyses in Patients with Age-Related Macular Degeneration and Diabetic Retinopathy","authors":"M. Akdogan, Y. Ustundağ, M. Sabaner, M. Doğan","doi":"10.5539/JMBR.V9N1P41","DOIUrl":"https://doi.org/10.5539/JMBR.V9N1P41","url":null,"abstract":"We evaluated biochemical analysis results with the aim of discovering serum levels that have possible effects and differences on age-related macular degeneration (AMD) and diabetic retinopathy (DRP). \u0000A retrospective case-control study was conducted between January 2017 and January 2018 on a total of 114 patients (84 DRP, 30 AMD) and 24 age and sex-matched control individuals. Four groups were created; 52 patients with proliferative DRP (PDR), 32 patients with nonproliferative PDR (non-PDR), 30 patients with wet AMD and 24 control individuals. Serum biochemical (HbA1C, fasting glucose, AST, ALT, C-reactive protein, albumin, total protein, uric acid, triglyceride, HDL, LDL, total cholesterol, Na, K, urine albumin) and complete blood count (CBC) analyses were performed at the time of diagnosis. Descriptive statistics, one-way ANOVA and Kruskal-Wallis tests were performed using SPSS to analyse data (Version 22.0). \u0000The mean age of patients was 63.3 years ± 6.4 (49-91year), and that of control individuals was 65.3 years ± 9.3 (50-88 year). Post hoc analysis showed statistically significant differences in HbA1c and fasting glucose levels among PDR-AMD, PDR-control, non-PDR-AMD, and non-PDR-control groups (whole, P","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48068487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Shams, M. Razavi, A. Mehrabi, S. Salehin, Parisa Sarikhani
Introduction: this study aimed at determining gender and age by mandibular anatomy landmarks in computed tomography with Cone-Beam (CBCT). Methodology: this cross sectional study was performed on 147 CBCT images available in archive of radiology in the dentistry department of Ahvaz Jondi Shapoor medical science university. In this research, we assessed parameters including SMEF: Distance from mental foramen to the highest point of alveolar crest ridge, BIAC: distance from lowest point of IAC to the most anterior tangent point of buccal mandibular plate, LIAC: distance from the lowest IAC point to the most posterior tangent point o mandibular lingual plate, IMEF: distance from the lowest mental hole border to the lowest tangent point on inferior mandibular border, D2: distance from the lowest IAC canal border to the lowest tangent point on inferior mandibular border and gonial angle: junction of inferior mandibular border and posterior ramus border. Data were analysed by SPSS software 20th version and Spearman correlation coefficient tests, one-way variance analysis, Kruskal-Wallis, independent t, and Uman Withney. Results: SMEF level was significantly different in groups and in 25-34 group it was significantly higher than under 25 group. In right side it was significantly higher than female. IMEF had no significant difference in age groups and in both side it was higher in male than female. BIAC in both sides had no significant difference. LIAC in both sides an in different ages had no significant difference in male and female. D2 had no significant difference in both sides. But in a group with patients older than 55 it was significantly higher than 45-54 group. In addition, in left side it was higher in male than female there was no significant difference in gonial angle in different groups in left side with in right side there was significant difference in different age groups. But there was no significant difference in gender. Conclusion: evaluated indices in this research are not ry accurate to forecast age and gender and they cannot be used as accurate tools in estimating age and gender of people.
{"title":"Determining Gender and Age by Mandibular Anatomy Landmarks in Computed Tomography with Cone-Beam (CBCT)","authors":"N. Shams, M. Razavi, A. Mehrabi, S. Salehin, Parisa Sarikhani","doi":"10.5539/JMBR.V9N1P33","DOIUrl":"https://doi.org/10.5539/JMBR.V9N1P33","url":null,"abstract":"Introduction: this study aimed at determining gender and age by mandibular anatomy landmarks in computed tomography with Cone-Beam (CBCT). \u0000 \u0000Methodology: this cross sectional study was performed on 147 CBCT images available in archive of radiology in the dentistry department of Ahvaz Jondi Shapoor medical science university. In this research, we assessed parameters including SMEF: Distance from mental foramen to the highest point of alveolar crest ridge, BIAC: distance from lowest point of IAC to the most anterior tangent point of buccal mandibular plate, LIAC: distance from the lowest IAC point to the most posterior tangent point o mandibular lingual plate, IMEF: distance from the lowest mental hole border to the lowest tangent point on inferior mandibular border, D2: distance from the lowest IAC canal border to the lowest tangent point on inferior mandibular border and gonial angle: junction of inferior mandibular border and posterior ramus border. Data were analysed by SPSS software 20th version and Spearman correlation coefficient tests, one-way variance analysis, Kruskal-Wallis, independent t, and Uman Withney. \u0000 \u0000Results: SMEF level was significantly different in groups and in 25-34 group it was significantly higher than under 25 group. In right side it was significantly higher than female. IMEF had no significant difference in age groups and in both side it was higher in male than female. BIAC in both sides had no significant difference. LIAC in both sides an in different ages had no significant difference in male and female. D2 had no significant difference in both sides. But in a group with patients older than 55 it was significantly higher than 45-54 group. In addition, in left side it was higher in male than female there was no significant difference in gonial angle in different groups in left side with in right side there was significant difference in different age groups. But there was no significant difference in gender. \u0000 \u0000Conclusion: evaluated indices in this research are not ry accurate to forecast age and gender and they cannot be used as accurate tools in estimating age and gender of people.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43520040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background & Aim. The aim of this study was to compare the bond strength of the orthodontic brackets bonded to the composite restorations following preparations by CO2 and Er;Cr:YSGG lasers and conventional phosphoric acid etching adult orthodontic treatment. Materials & Methods. Class V cavities were prepared on the buccal surfaces of 60 acrylic teeth and restored by composite after etching by 37% acid-etch gel. The specimens’ surfaces were prepared randomly by 37% phosphoric acid etching or Er;Cr:YSGG or CO2 lasers. Central metal brackets were installed on the teeth's surfaces. The shear bond strength of the brackets to composite surfaces was measured by the crosshead speed of 1mm/min. The scores of the remaining adhesive on the surfaces were calculated by ARI index in 5 scales. The shear bond strength values and the ARI scores were analyzed by one-way ANOVA and Chi-square tests respectively. Results. There were no significant differences among the surface preparation methods regarding bond strength between composite surfaces and the brackets. Most specimens showed ARI index of 3 in the acid phosphoric etching. In CO2 laser application, ARI index of 2 and 3 were more frequent. In Er;Cr:YSGG laser, ARI index of 3 was predominant. No significant differences existed among 3 modalities regarding scores of ARI index. Conclusion. Irradiation of CO2 and Er;Cr:YSGG lasers is recommended for clinical applications due to adequate bond strength created between the brackets and composite surfaces as well as advantages such as lower chair time and no damage to the gingival tissues.
{"title":"Comparison of Shear Bond Strength of Orthodontic Metal Brackets Adhered to Composite Restorations Pretreated with Er;Cr:YSGG and CO2 Lasers and Phosphoric Acid","authors":"M. Mofateh, M. Khanemasjedi, Meysam Noori","doi":"10.5539/jmbr.v9n1p14","DOIUrl":"https://doi.org/10.5539/jmbr.v9n1p14","url":null,"abstract":"Background & Aim. The aim of this study was to compare the bond strength of the orthodontic brackets bonded to the composite restorations following preparations by CO2 and Er;Cr:YSGG lasers and conventional phosphoric acid etching adult orthodontic treatment. Materials & Methods. Class V cavities were prepared on the buccal surfaces of 60 acrylic teeth and restored by composite after etching by 37% acid-etch gel. The specimens’ surfaces were prepared randomly by 37% phosphoric acid etching or Er;Cr:YSGG or CO2 lasers. Central metal brackets were installed on the teeth's surfaces. The shear bond strength of the brackets to composite surfaces was measured by the crosshead speed of 1mm/min. The scores of the remaining adhesive on the surfaces were calculated by ARI index in 5 scales. The shear bond strength values and the ARI scores were analyzed by one-way ANOVA and Chi-square tests respectively. Results. There were no significant differences among the surface preparation methods regarding bond strength between composite surfaces and the brackets. Most specimens showed ARI index of 3 in the acid phosphoric etching. In CO2 laser application, ARI index of 2 and 3 were more frequent. In Er;Cr:YSGG laser, ARI index of 3 was predominant. No significant differences existed among 3 modalities regarding scores of ARI index. Conclusion. Irradiation of CO2 and Er;Cr:YSGG lasers is recommended for clinical applications due to adequate bond strength created between the brackets and composite surfaces as well as advantages such as lower chair time and no damage to the gingival tissues.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45632574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Eluka-Okoludoh, K. Ekwemalor, S. Adjei-Fremah, B. Mulakala, M. Worku
Galectins (Gals) are a family of animal lectins that bind β-galactosides through a carbohydrate recognition domain. Galectin-8 is a tandem-repeat galectin, secreted intracellularly and extracellularly. It is associated with neutrophil migration and has been studied as a possible therapeutic to combat inflammation. The objective of this study was to evaluate the translational and the transcriptional effects of recombinant Galectin-8 (rGal-8) on cow neutrophils. Blood was collected aseptically from Holstein-Friesian cows (n=10) from the North Carolina A&T State University Dairy Unit. Neutrophils isolated were treated with rGal-8 (2μg), or PBS (control) and were incubated at 37°C, 5% CO2 for 1 hour. Supernatant from treated neutrophils was evaluated for total protein concentration, and galectin-8 secretion using bovine Galectin-8 Enzyme Linked-Immuno-Sorbent Assay (ELISA) kit. Total RNA was extracted, reverse transcribed, and RT-qPCR was performed using the RT² Profiler Cow Innate & Adaptive Immune Responses Array with 84 genes. The Livak method was used to calculate transcript abundance and fold change (FC>2 considered significant). Total protein concentration increased (P=0.0361) after rGal-8 treatment compared to the untreated control. Galectin-8 secretion was not significantly different in control compared to treated group (P=0.5819). Out of the 84 genes, 81 genes were differentially expressed in response to rGal-8; 14 up-regulated, 5 down-regulated, 61 genes remained unchanged. Treatment with rGal8 induced the expression of IRF7. The top five up-regulated genes include FAS, CD40, CD86, IFNGR1, STAT1; down-regulated genes were TLR9, CD14, CCR6, TICAM1, and TLR1. Selected genes were probed to validate fold change; the levels of gene expression were comparable to data from RT2 array. Exposure of bovine neutrophils to rGal-8 modified expression of immune response genes. The functional significance of the change needs further studies.
{"title":"Galectin-8 Modulates Innate and Adaptive Immune Response Genes in Bovine Neutrophils","authors":"E. Eluka-Okoludoh, K. Ekwemalor, S. Adjei-Fremah, B. Mulakala, M. Worku","doi":"10.5539/JMBR.V9N1P24","DOIUrl":"https://doi.org/10.5539/JMBR.V9N1P24","url":null,"abstract":"Galectins (Gals) are a family of animal lectins that bind β-galactosides through a carbohydrate recognition domain. Galectin-8 is a tandem-repeat galectin, secreted intracellularly and extracellularly. It is associated with neutrophil migration and has been studied as a possible therapeutic to combat inflammation. The objective of this study was to evaluate the translational and the transcriptional effects of recombinant Galectin-8 (rGal-8) on cow neutrophils. Blood was collected aseptically from Holstein-Friesian cows (n=10) from the North Carolina A&T State University Dairy Unit. Neutrophils isolated were treated with rGal-8 (2μg), or PBS (control) and were incubated at 37°C, 5% CO2 for 1 hour. Supernatant from treated neutrophils was evaluated for total protein concentration, and galectin-8 secretion using bovine Galectin-8 Enzyme Linked-Immuno-Sorbent Assay (ELISA) kit. Total RNA was extracted, reverse transcribed, and RT-qPCR was performed using the RT² Profiler Cow Innate & Adaptive Immune Responses Array with 84 genes. The Livak method was used to calculate transcript abundance and fold change (FC>2 considered significant). Total protein concentration increased (P=0.0361) after rGal-8 treatment compared to the untreated control. Galectin-8 secretion was not significantly different in control compared to treated group (P=0.5819). Out of the 84 genes, 81 genes were differentially expressed in response to rGal-8; 14 up-regulated, 5 down-regulated, 61 genes remained unchanged. Treatment with rGal8 induced the expression of IRF7. The top five up-regulated genes include FAS, CD40, CD86, IFNGR1, STAT1; down-regulated genes were TLR9, CD14, CCR6, TICAM1, and TLR1. Selected genes were probed to validate fold change; the levels of gene expression were comparable to data from RT2 array. Exposure of bovine neutrophils to rGal-8 modified expression of immune response genes. The functional significance of the change needs further studies.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48615631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Elahe Bazri, S. Khorram, M. Mesgari, M. Asghari-Jafarabadi, A. Tarighat-Esfanjani, Yalda Salari
The inflammatory efficacy of supplementation of natural nano-sized clinoptilolite (NCLN) and Nigella sativa (NS) was evaluated At the end of 7th week in interleukin1β (IL1β), interleukin10 (IL-10), interleukin6 (IL-6) and platelet (PLT), white blood cell (WBC), red blood cell (RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular volume (MCV). 42 rats were divided into two groups as diabetic and non-diabetic. Diabetic group divided into 4 subgroups as normal control (NC), NS 1% food, NCLN 2%/food, NS 1%/food + NCLN 2%/food and fed high fat diet (HFD) for 1 month, then injected 35mg/kg BW STZ to induce type 2 diabetes (T2D). Our results showed IL-10 in NCLN and NCLN+NS groups were significantly higher NC group (p<0.05). IL-6 decreased in NS group in comparison with DC group. And PLT decreased significantly in NCLN group in comparison with other groups. Our data suggests NS and NCLN may have synergistic beneficial effects on increasing some of anti-inflammatory factors.
{"title":"Synergistic Effects of Nano-Sized Clinoptilolite and Nigella Sativa on Inflammatory and Hematological Factors in Rats with Type 2 Diabetes","authors":"Elahe Bazri, S. Khorram, M. Mesgari, M. Asghari-Jafarabadi, A. Tarighat-Esfanjani, Yalda Salari","doi":"10.5539/JMBR.V9N1P7","DOIUrl":"https://doi.org/10.5539/JMBR.V9N1P7","url":null,"abstract":"The inflammatory efficacy of supplementation of natural nano-sized clinoptilolite (NCLN) and Nigella sativa (NS) was evaluated At the end of 7th week in interleukin1β (IL1β), interleukin10 (IL-10), interleukin6 (IL-6) and platelet (PLT), white blood cell (WBC), red blood cell (RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular volume (MCV). 42 rats were divided into two groups as diabetic and non-diabetic. Diabetic group divided into 4 subgroups as normal control (NC), NS 1% food, NCLN 2%/food, NS 1%/food + NCLN 2%/food and fed high fat diet (HFD) for 1 month, then injected 35mg/kg BW STZ to induce type 2 diabetes (T2D). Our results showed IL-10 in NCLN and NCLN+NS groups were significantly higher NC group (p<0.05). IL-6 decreased in NS group in comparison with DC group. And PLT decreased significantly in NCLN group in comparison with other groups. Our data suggests NS and NCLN may have synergistic beneficial effects on increasing some of anti-inflammatory factors.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42609610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Takzare, A. Maleki, M. Goudarzi, A. E. Soltani, Irandokht Ghorbani Sepehr
Objective: Acute pain is a common complication in children undergoing surgery. Acute pain and delirium are associated with agitated behavior, in which there is a risk that the patient causes harm to himself and others. Acute pain and delirium also dissatisfy parents and caregivers during recovery, prolong the recovery and hospitalization, and make more nursing care necessary. Material and Methods: In this study, 90 ASA I children, aged 3-8, and two candidates for elective inguinal hernia surgery were divided into three groups based on a random number table using block size of four. Parental permission was obtained beforehand. In group 1, 1mg/kg of ketamine (3cc) was administered orally. In group 2, 0.5mg/kg of ketamine was administered subcutaneously after the anesthetic induction. In Group 3, or control group, the same amount of normal saline was administered as the placebo, then the surgery got underway. Results: Comparing the administration of oral ketamine, subcutaneous ketamine and normal saline, the study showed that the pain was significantly higher in the placebo group at 30, 60, 90 and 120 minutes. Subcutaneous ketamine resulted in higher delirium at 30, 60, 90 and 120 minutes than the placebo and oral ketamine. Satisfaction of nurses from the patient’s recovery was significantly higher in the oral ketamine group and subcutaneous ketamine group (p=0/007). The need for narcotics in the oral ketamine group and subcutaneous ketamine group was lower than the placebo group (p=0/001). Ketamine administration prolonged the length of stay in the PACU, but it did not increase the frequency of complications such as postoperative nausea and vomiting and laryngospasm or bronchospasm in the two groups during the recovery. Conclusion: In conclusion, the study indicated that oral administration of low-dose ketamine before surgery can reduce pain severity and spare the need for analgesics after surgery.
{"title":"A Comparative Study on the Effect of Low-Dose Oral Ketamine and Subcutaneous Ketamine on Post General Anesthesia Delirium and Pain in Children Undergoing Inguinal Hernia Surgery","authors":"A. Takzare, A. Maleki, M. Goudarzi, A. E. Soltani, Irandokht Ghorbani Sepehr","doi":"10.5539/jmbr.v9n1p1","DOIUrl":"https://doi.org/10.5539/jmbr.v9n1p1","url":null,"abstract":"Objective: Acute pain is a common complication in children undergoing surgery. Acute pain and delirium are associated with agitated behavior, in which there is a risk that the patient causes harm to himself and others. Acute pain and delirium also dissatisfy parents and caregivers during recovery, prolong the recovery and hospitalization, and make more nursing care necessary. \u0000 \u0000Material and Methods: In this study, 90 ASA I children, aged 3-8, and two candidates for elective inguinal hernia surgery were divided into three groups based on a random number table using block size of four. Parental permission was obtained beforehand. In group 1, 1mg/kg of ketamine (3cc) was administered orally. In group 2, 0.5mg/kg of ketamine was administered subcutaneously after the anesthetic induction. In Group 3, or control group, the same amount of normal saline was administered as the placebo, then the surgery got underway. \u0000 \u0000Results: Comparing the administration of oral ketamine, subcutaneous ketamine and normal saline, the study showed that the pain was significantly higher in the placebo group at 30, 60, 90 and 120 minutes. Subcutaneous ketamine resulted in higher delirium at 30, 60, 90 and 120 minutes than the placebo and oral ketamine. Satisfaction of nurses from the patient’s recovery was significantly higher in the oral ketamine group and subcutaneous ketamine group (p=0/007). The need for narcotics in the oral ketamine group and subcutaneous ketamine group was lower than the placebo group (p=0/001). Ketamine administration prolonged the length of stay in the PACU, but it did not increase the frequency of complications such as postoperative nausea and vomiting and laryngospasm or bronchospasm in the two groups during the recovery. \u0000 \u0000Conclusion: In conclusion, the study indicated that oral administration of low-dose ketamine before surgery can reduce pain severity and spare the need for analgesics after surgery.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44594033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biao Wu, Menglong Li, Libing Zheng, Zhihong Liu, A. Yang, Xiujun Sun, Liqing Zhou, Chao Sun
As one crucial member of heat shock proteins (HSPs) family, HSP70s play many important roles in a large amount of physiological processes including immune response. However, information regarding HSP70 in ark shell Scapharca broughtonii is still rather limited. Here the full-length cDNA of HSP70 gene (named SbHSP70) of S. broughtonii was identified by using reverse transcription PCR (RT-PCR) and rapid amplification ends (RACE) methods. The SbHSP70 cDNA was 2423 bp in length containing a 5′ untranslated region (UTR) of 131 bp, 3′-UTR of 330 bp, and an open reading frame (ORF) of 1962 bp which encodes a peptide of 653 amino acids.The multiple alignment and phylogeny analysis showed that the SbHSP70 shared high homology sequence with other mollusk species, and clustered together with gastropods to form a sister group. The mRNA expression profiles of SbHSP70 in tissues of foot, gill, mantle, adductor muscle, haemocytes and hepatopancreas analyzed by quantitative real-time PCR (qRT-PCR) suggested the mRNA transcripts of SbHSP70 distributed in all the examined tissues, and the highest expression level was observed in foot, and a significant difference could be detected between gill and adductor muscle (p<0.05), no significant difference among the gill, mantle and hemocytes (p > 0.05). Its dynamic change during the early stage of larvae showed that it could be transferred from parent and may be involved in some key developmental process. What’s more, Vibrio anguillarum challenge resulted in regular change of expression of SbHSP70 mRNA, indicating SbHSP70 actively participated in the immune response process.
{"title":"Identification, Characterization and Expression of Heat Shock Protein 70 in Scapharca broughtonii","authors":"Biao Wu, Menglong Li, Libing Zheng, Zhihong Liu, A. Yang, Xiujun Sun, Liqing Zhou, Chao Sun","doi":"10.5539/JMBR.V8N1P167","DOIUrl":"https://doi.org/10.5539/JMBR.V8N1P167","url":null,"abstract":"As one crucial member of heat shock proteins (HSPs) family, HSP70s play many important roles in a large amount of physiological processes including immune response. However, information regarding HSP70 in ark shell Scapharca broughtonii is still rather limited. Here the full-length cDNA of HSP70 gene (named SbHSP70) of S. broughtonii was identified by using reverse transcription PCR (RT-PCR) and rapid amplification ends (RACE) methods. The SbHSP70 cDNA was 2423 bp in length containing a 5′ untranslated region (UTR) of 131 bp, 3′-UTR of 330 bp, and an open reading frame (ORF) of 1962 bp which encodes a peptide of 653 amino acids.The multiple alignment and phylogeny analysis showed that the SbHSP70 shared high homology sequence with other mollusk species, and clustered together with gastropods to form a sister group. The mRNA expression profiles of SbHSP70 in tissues of foot, gill, mantle, adductor muscle, haemocytes and hepatopancreas analyzed by quantitative real-time PCR (qRT-PCR) suggested the mRNA transcripts of SbHSP70 distributed in all the examined tissues, and the highest expression level was observed in foot, and a significant difference could be detected between gill and adductor muscle (p<0.05), no significant difference among the gill, mantle and hemocytes (p > 0.05). Its dynamic change during the early stage of larvae showed that it could be transferred from parent and may be involved in some key developmental process. What’s more, Vibrio anguillarum challenge resulted in regular change of expression of SbHSP70 mRNA, indicating SbHSP70 actively participated in the immune response process.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45726109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Osei, M. Worku, S. Adjei-Fremah, E. Asiamah, K. Ekwemalor, E. Eluka-Okoludoh, B. Mulakala
Galectins (GAL) are β-galactoside binding proteins that can modulate both pregnancy and the response to pathogens. Increased susceptibility to infection by pathogens is linked to periparturient immune suppression and a periparturient rise (PPR) in parasite eggs on pasture. The possible role of GAL in periparturient immune relaxation and PRR needs definition. The objective of this study was to evaluate galectin secretion and its relationship to measures of the immune relaxation and PRR in periparturient sheep. Samples were collected from pregnant St. Croix sheep (n=6) weekly at days -21 to + 21 relative to lambing. Fecal samples were collected and evaluated for strongyle and coccidia parasite eggs. The concentration of IgA and IgE coproantibodies, total microbial DNA, Bifidobacteria and Lactobacillus levels in fecal samples were used as indicators of gut health. Blood samples were collected by jugular venipuncture and assessed for Packed Cell Volume (PCV), total and differential white blood cell counts. Total protein concentrations and protein profile were evaluated in serum. Secretion of GAL 1, 3, 9 and 14 were evaluated using ELISAs. Data were analyzed by one-way ANOVA and statistical significance was declared at P <0.05. Galectins tested were secreted in sheep blood. Differential modulation of GAL secretion and correlation with periparturient immune suppression and parasite infection was observed. Galectin secretion was modulated by the periparturient period, type and status of parasite infection. This first insight into a possible role of secreted galectins in periparturient immune relaxation and PRR improves the understanding of the immune response, informs development of management programs and therapeutics and presents Galectin profiles as biomarkers with diagnostic potential.
{"title":"Galectin Secretion and Modulation in Sheep Blood","authors":"B. Osei, M. Worku, S. Adjei-Fremah, E. Asiamah, K. Ekwemalor, E. Eluka-Okoludoh, B. Mulakala","doi":"10.5539/JMBR.V8N1P183","DOIUrl":"https://doi.org/10.5539/JMBR.V8N1P183","url":null,"abstract":"Galectins (GAL) are β-galactoside binding proteins that can modulate both pregnancy and the response to pathogens. Increased susceptibility to infection by pathogens is linked to periparturient immune suppression and a periparturient rise (PPR) in parasite eggs on pasture. The possible role of GAL in periparturient immune relaxation and PRR needs definition. The objective of this study was to evaluate galectin secretion and its relationship to measures of the immune relaxation and PRR in periparturient sheep. Samples were collected from pregnant St. Croix sheep (n=6) weekly at days -21 to + 21 relative to lambing. Fecal samples were collected and evaluated for strongyle and coccidia parasite eggs. The concentration of IgA and IgE coproantibodies, total microbial DNA, Bifidobacteria and Lactobacillus levels in fecal samples were used as indicators of gut health. Blood samples were collected by jugular venipuncture and assessed for Packed Cell Volume (PCV), total and differential white blood cell counts. Total protein concentrations and protein profile were evaluated in serum. Secretion of GAL 1, 3, 9 and 14 were evaluated using ELISAs. Data were analyzed by one-way ANOVA and statistical significance was declared at P <0.05. Galectins tested were secreted in sheep blood. Differential modulation of GAL secretion and correlation with periparturient immune suppression and parasite infection was observed. Galectin secretion was modulated by the periparturient period, type and status of parasite infection. This first insight into a possible role of secreted galectins in periparturient immune relaxation and PRR improves the understanding of the immune response, informs development of management programs and therapeutics and presents Galectin profiles as biomarkers with diagnostic potential.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41460653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Moradpoor, Farshad Rahimi, A. Golshah, N. Akbari, S. Raissi
Introduction: Due to the fundamental role of esthetics in the outcomes of dental treatments, especially in the anterior region (esthetic zone), the necessity of considering the matter of esthetics in clinical studies has become into focus in the current era. The aim of this study was the evaluation of esthetic outcomes of two treatment protocols in the treatment of congenital uni-lateral missing of maxillary lateral incisors as well as patient satisfaction from the treatment outcomes. Methods: in this study the sample size was 24 people (16 women and 8 men), These individuals sought dental treatment for replacement of the congenitally missing maxillary lateral incisor. Convenience sampling method was used and patients were divided into two groups regarding the kind of treatment they received. The two treatment protocols included: 1. Space closure by means of orthodontic treatment and then reshaping the canines; and 2. Space regaining by means of orthodontic treatment and replacing the lateral incisor with dental implants. Photographs of patients were acquired from the frontal view with retraction of the lips using digital cameras. Photographs were evaluated for Pink esthetic score. Results: No significant difference was detected between the two study groups in the evaluated factors in this study. Conclusion: The results of this study indicated that there is no significant difference in esthetic results in the two groups. Furthermore, both groups lead to similar results in patient satisfaction from treatment outcomes.
{"title":"Comparison of Esthetic Outcomes of Maxillary Lateral Incisor Agenesis Treatment by Orthodontic Space Closure Versus Implant Placement (Evaluated by Pink Esthetic Score)","authors":"H. Moradpoor, Farshad Rahimi, A. Golshah, N. Akbari, S. Raissi","doi":"10.5539/jmbr.v8n1p178","DOIUrl":"https://doi.org/10.5539/jmbr.v8n1p178","url":null,"abstract":"Introduction: Due to the fundamental role of esthetics in the outcomes of dental treatments, especially in the anterior region (esthetic zone), the necessity of considering the matter of esthetics in clinical studies has become into focus in the current era. The aim of this study was the evaluation of esthetic outcomes of two treatment protocols in the treatment of congenital uni-lateral missing of maxillary lateral incisors as well as patient satisfaction from the treatment outcomes. \u0000 \u0000Methods: in this study the sample size was 24 people (16 women and 8 men), These individuals sought dental treatment for replacement of the congenitally missing maxillary lateral incisor. Convenience sampling method was used and patients were divided into two groups regarding the kind of treatment they received. The two treatment protocols included: 1. Space closure by means of orthodontic treatment and then reshaping the canines; and 2. Space regaining by means of orthodontic treatment and replacing the lateral incisor with dental implants. Photographs of patients were acquired from the frontal view with retraction of the lips using digital cameras. Photographs were evaluated for Pink esthetic score. \u0000 \u0000Results: No significant difference was detected between the two study groups in the evaluated factors in this study. \u0000 \u0000Conclusion: The results of this study indicated that there is no significant difference in esthetic results in the two groups. Furthermore, both groups lead to similar results in patient satisfaction from treatment outcomes.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42253350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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