A. Verghese, J. Arora, Uma Sharma, A. Rai, D. Chattopadhya
Despite common incidence of GB virus C/Hepatitis G virus (GBV-C/HGV) and Torque Teno Virus (TTV) co-infections in subjects with Hepatitis C virus (HCV) infection, pathogenic role of these co-infections in HCV infected subjects has not been clear since studies have mostly been based on liver enzyme profile yielding variable results. Pro-inflammatory cytokines that participate in host immune response against viruses are generated as a consequence of triggering of related genes by Nuclear Factor Kappa B (NF-kB) that is a member of transcription family. Study of three pro-inflammatory cytokines e.g. interleukin 1 beta (IL-1β), Interleukin-6 (IL-6), interleukin-8 (IL-8) in a group of multi-transfused thalassemic subjects in relation to positivity for HCV, HGV and TTV infections showed elevated levels of these cytokines in serum as well as in supernatant of peripheral blood mononuclear cell (PBMCs) cultures in HCV-GBV-C/HGV co-infected subgroup compared to HCV mono-infected subgroup (p <0.05 for comparison of all three cytokines) while HCV-TTV co-infected subgroup showed lowering of these levels compared to HCV mono-infected subgroup (p <0.05 for comparison of all three cytokines). Levels of p65 component of NF-kB i.e.NF-kB p65 in nuclear extracts of lipopolysaccharide stimulated PBMCs correlated positively with the levels of pro-inflammatory cytokines in serum as well as that in supernatants of PBMC cultures in both HCV- GBV-C/HGV co-infected and HCV-TTV co-infected subgroups (p values ranging from <0.001 to 0.004). Levels of NF-kB p65 in nuclear extracts and levels of pro-inflammatory cytokines in serum as well as in supernatants of PBMC culture did not show any alteration compared to thalassemic subjects with HGV or TTV infections alone and healthy non-transfused subjects. Based on the inhibitory role of TTV on activation of NF-kB in TTV-HCV co-infected cases observed in the present study and the reported contribution of NF-kB towards development of hepatocellular carcinoma (HCC) due to establishment of chronicity, it may be worth evaluating if TTV or any component of TTV can be utilized as therapeutic vaccine against development of HCC in HCV infected subjects.
尽管GB病毒C/ G型肝炎病毒(GBV-C/HGV)和Torque Teno病毒(TTV)合并感染在丙型肝炎病毒(HCV)感染的受试者中发生率很高，但这些合并感染在HCV感染受试者中的致病作用尚不清楚，因为研究大多基于肝酶谱，结果不同。参与宿主对病毒免疫应答的促炎细胞因子是转录家族成员核因子κ B (NF-kB)触发相关基因的结果。三种促炎细胞因子如白细胞介素1β (IL-1β)、白细胞介素6 (IL-6)、白细胞介素8 (IL-8)与多次输血地中海贫血患者HCV阳性的关系研究在HCV- gbv - c /HGV共感染亚组中，与HCV单感染亚组相比，HCV- gbv - c /HGV共感染亚组的血清和外周血单核细胞培养上清中这些细胞因子的水平升高(三种细胞因子的比较p <0.05)，而HCV-TTV共感染亚组与HCV单感染亚组相比，这些水平降低(三种细胞因子的比较p <0.05)。在HCV- GBV-C/ HCV- ttv共感染亚组和HCV- GBV-C/ HCV共感染亚组中，NF-kB p65成分即脂多糖刺激的PBMC核提取物中NF-kB p65的水平与血清中促炎细胞因子水平以及PBMC培养上清液中促炎细胞因子水平呈正相关(p值<0.001 ~ 0.004)。与单纯感染HGV或TTV的地中海贫血患者和未输血的健康受试者相比，核提取物中NF-kB p65水平和血清中促炎细胞因子水平以及PBMC培养上清液中未显示出任何变化。基于本研究中观察到的TTV对TTV-HCV共感染病例中NF-kB激活的抑制作用，以及NF-kB因慢性建立而对肝细胞癌(HCC)发展的贡献，TTV或TTV的任何成分是否可以用作抗HCV感染者HCC发展的治疗性疫苗，可能值得评估。
{"title":"Divergent Pro-Inflammatory Cytokine Response Induced by GB Virus C/ Hepatitis G virus (GBV-C/HGV) and Torque Teno Virus (TTV) Co-Infections in Hepatitis C Virus (HCV) Infected Thalassemia Patients","authors":"A. Verghese, J. Arora, Uma Sharma, A. Rai, D. Chattopadhya","doi":"10.5539/JMBR.V8N1P137","DOIUrl":"https://doi.org/10.5539/JMBR.V8N1P137","url":null,"abstract":"Despite common incidence of GB virus C/Hepatitis G virus (GBV-C/HGV) and Torque Teno Virus (TTV) co-infections in subjects with Hepatitis C virus (HCV) infection, pathogenic role of these co-infections in HCV infected subjects has not been clear since studies have mostly been based on liver enzyme profile yielding variable results. Pro-inflammatory cytokines that participate in host immune response against viruses are generated as a consequence of triggering of related genes by Nuclear Factor Kappa B (NF-kB) that is a member of transcription family. Study of three pro-inflammatory cytokines e.g. interleukin 1 beta (IL-1β), Interleukin-6 (IL-6), interleukin-8 (IL-8) in a group of multi-transfused thalassemic subjects in relation to positivity for HCV, HGV and TTV infections showed elevated levels of these cytokines in serum as well as in supernatant of peripheral blood mononuclear cell (PBMCs) cultures in HCV-GBV-C/HGV co-infected subgroup compared to HCV mono-infected subgroup (p <0.05 for comparison of all three cytokines) while HCV-TTV co-infected subgroup showed lowering of these levels compared to HCV mono-infected subgroup (p <0.05 for comparison of all three cytokines). Levels of p65 component of NF-kB i.e.NF-kB p65 in nuclear extracts of lipopolysaccharide stimulated PBMCs correlated positively with the levels of pro-inflammatory cytokines in serum as well as that in supernatants of PBMC cultures in both HCV- GBV-C/HGV co-infected and HCV-TTV co-infected subgroups (p values ranging from <0.001 to 0.004). Levels of NF-kB p65 in nuclear extracts and levels of pro-inflammatory cytokines in serum as well as in supernatants of PBMC culture did not show any alteration compared to thalassemic subjects with HGV or TTV infections alone and healthy non-transfused subjects. Based on the inhibitory role of TTV on activation of NF-kB in TTV-HCV co-infected cases observed in the present study and the reported contribution of NF-kB towards development of hepatocellular carcinoma (HCC) due to establishment of chronicity, it may be worth evaluating if TTV or any component of TTV can be utilized as therapeutic vaccine against development of HCC in HCV infected subjects.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48039918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of the present work is to follow the capacity of adsorption of CO2 by natural cellulose nanofibers, extracted from banana peel and mixed with nano-silver. Following initial characterization, the raw material and the isolated sample were investigated by Energy Dispersive Analysis (EDS), X-ray diffraction (XRD); Fourier- Transformed infrared spectroscopy (FT-IR) and thermal analysis (Hi Res TG). Nitrogen gas adsorption-desorption isotherms of isolated cellulose nanofibers reveal a type I- isotherm, specific to the nano-porous materials.The results of adsorption isotherms of CO2 at 304 K showed that the extracted sample exhibit an adsorption capacity of 12 mmol/g of CO2.
研究了从香蕉皮中提取的天然纤维素纳米纤维与纳米银混合后对CO2的吸附性能。初步表征后，采用能谱分析(EDS)、x射线衍射(XRD)对原料和分离样品进行了表征;傅里叶变换红外光谱(FT-IR)和热分析(Hi Res TG)。分离的纤维素纳米纤维的氮气吸附-解吸等温线显示为I型等温线，这是纳米多孔材料所特有的。304 K下CO2吸附等温线结果表明，提取的样品对CO2的吸附量为12 mmol/g。
{"title":"Synthesis of New Porous Natural Membranes with Nanoparticles Used in Pollute Gas Removal From Petroleum Industries","authors":"R. Ahmed, K. Belgacem, H. Abo-Dief","doi":"10.5539/JMBR.V8N1P159","DOIUrl":"https://doi.org/10.5539/JMBR.V8N1P159","url":null,"abstract":"The aim of the present work is to follow the capacity of adsorption of CO2 by natural cellulose nanofibers, extracted from banana peel and mixed with nano-silver. Following initial characterization, the raw material and the isolated sample were investigated by Energy Dispersive Analysis (EDS), X-ray diffraction (XRD); Fourier- Transformed infrared spectroscopy (FT-IR) and thermal analysis (Hi Res TG). Nitrogen gas adsorption-desorption isotherms of isolated cellulose nanofibers reveal a type I- isotherm, specific to the nano-porous materials.The results of adsorption isotherms of CO2 at 304 K showed that the extracted sample exhibit an adsorption capacity of 12 mmol/g of CO2.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41425659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zakiyeh Donyavi, M. Arabestani, D. Dastan, M. Esmaeilzadeh, Nazanin Shahsavand
Background and Aim: The present study investigated the antimicrobial effect of Berberine as an endodontic irrigant on the microorganisms involved in persistent endodontic infections. In this experimental in vitro trial, organisms Enterococcus Faecalis, Staphylococcus Aureus, and Staphylococcus Epidermidis were assessed in a multi-species biofilm tooth model. Methods: Seventy-five single-rooted anterior teeth were collected and standardized to a length of 10mm. The teeth were randomly assigned into 5 groups. The teeth were then autoclaved to confirm being sterile. Afterwards, a biofilm consisting of the three selected bacteria was inoculated into the teeth and they were incubated for 21 days. Results: The comparison of the amount of reduction in viable bacterial counts after irrigation by different solutions among the groups was done by Kruskal-wallis test while the changes of viable bacterial counts before and after irrigation with each solution was done by Wilocoxon Signed Ranks test. No significant difference existed among the studied irrigation solutions regarding the mean reduction of total viable bacterial counts, neither did it exist regarding the viable staphylococcus aureus, staphylococcus epidermidis or Enterococcus faecalis counts after irrigation. MIC of berberine against staphylococcus aureus, staphylococcus epidermidis and Enterococcus faecalis species were 0.5ug/ml, 0.03ug/ml and 1ug/ml, respectively. These values were found to be 0.39ug/ml, 0.09ug/ml and 0.78ug/ml for NaOCl and 0.04ug/ml, 0.04ug/ml and 0.09ug/ml for chlorhexidine, respectively. Conclusion: it is concluded that Berberine can be used as a natural alternative instead of conventional root canal irrigants. However, more studies are required in order to confirm that characteristics of this substance are appropriate from other aspects.
{"title":"Comparison of Antimicrobial Effect of Berberine as an Endodontic Irrigant with that of Other Common Root Canal Irrigants on Three Microorganisms Involved in Persistent Endodontic Infections","authors":"Zakiyeh Donyavi, M. Arabestani, D. Dastan, M. Esmaeilzadeh, Nazanin Shahsavand","doi":"10.5539/jmbr.v8n1p153","DOIUrl":"https://doi.org/10.5539/jmbr.v8n1p153","url":null,"abstract":"Background and Aim: The present study investigated the antimicrobial effect of Berberine as an endodontic irrigant on the microorganisms involved in persistent endodontic infections. In this experimental in vitro trial, organisms Enterococcus Faecalis, Staphylococcus Aureus, and Staphylococcus Epidermidis were assessed in a multi-species biofilm tooth model. \u0000 \u0000Methods: Seventy-five single-rooted anterior teeth were collected and standardized to a length of 10mm. The teeth were randomly assigned into 5 groups. The teeth were then autoclaved to confirm being sterile. Afterwards, a biofilm consisting of the three selected bacteria was inoculated into the teeth and they were incubated for 21 days. \u0000 \u0000Results: The comparison of the amount of reduction in viable bacterial counts after irrigation by different solutions among the groups was done by Kruskal-wallis test while the changes of viable bacterial counts before and after irrigation with each solution was done by Wilocoxon Signed Ranks test. No significant difference existed among the studied irrigation solutions regarding the mean reduction of total viable bacterial counts, neither did it exist regarding the viable staphylococcus aureus, staphylococcus epidermidis or Enterococcus faecalis counts after irrigation. MIC of berberine against staphylococcus aureus, staphylococcus epidermidis and Enterococcus faecalis species were 0.5ug/ml, 0.03ug/ml and 1ug/ml, respectively. These values were found to be 0.39ug/ml, 0.09ug/ml and 0.78ug/ml for NaOCl and 0.04ug/ml, 0.04ug/ml and 0.09ug/ml for chlorhexidine, respectively. \u0000 \u0000Conclusion: it is concluded that Berberine can be used as a natural alternative instead of conventional root canal irrigants. However, more studies are required in order to confirm that characteristics of this substance are appropriate from other aspects.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43319279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The evolutionarily conserved Wnt signaling pathway plays essential roles during embryonic development, tissue homeostasis and differentiation. This pathway is deregulated in many cancers especially colorectal cancer. MiRNAs are a class of small noncoding RNAs that may play a major role in post transcriptional regulation of many genes and signaling pathway such as WNT signaling pathway. Here, we intended to investigate if miR-186-5p is capable of regulating WNT signaling pathway wia suppression TCF4 gene expression. miR-186-5p was bioinformatically predicted as a candidate regulator of TCF4 gene expression and then, in this experimental study, miR-186-5p was overexpressed in SW480 cell line and its increased expression was detected through quantitative reverse transcription polymerase chain reaction (RT-qPCR). The effect of miR-186-5p on WNT pathway was analysied with TOP/FOP flash assay in SW480 cell line. Finally, flow cytometery was used to inves tigate the effect of miR-186-5p overexpression on cell cycle progression in SW480 cell line. miR-186-5p was overexpressed in the SW480 cell line and its overexpression resulted in significant reduction of the TCF4 mRNA level. TOP/FOP flash assay, confirmed the negative effect of miR-186-5p on the Wnt pathway in SW480 cells. Finally, Overexpression of miR186-5p in SW480 cells resulted in cell cycle arrest in subG1 phase, detected by flow cytometry. Overall, accumulative results indi-cated that miR-186-5p by targeting TCF4 is potentially one of the regulators of the WNT signaling pathway.
{"title":"Mir-186-5p Regulates WNT Signaling Pathway by Targeting TCF4 Transcription Factor","authors":"Z. Bayat, B. Soltani","doi":"10.5539/jmbr.v8n1p132","DOIUrl":"https://doi.org/10.5539/jmbr.v8n1p132","url":null,"abstract":"The evolutionarily conserved Wnt signaling pathway plays essential roles during embryonic development, tissue homeostasis and differentiation. This pathway is deregulated in many cancers especially colorectal cancer. MiRNAs are a class of small noncoding RNAs that may play a major role in post transcriptional regulation of many genes and signaling pathway such as WNT signaling pathway. Here, we intended to investigate if miR-186-5p is capable of regulating WNT signaling pathway wia suppression TCF4 gene expression. miR-186-5p was bioinformatically predicted as a candidate regulator of TCF4 gene expression and then, in this experimental study, miR-186-5p was overexpressed in SW480 cell line and its increased expression was detected through quantitative reverse transcription polymerase chain reaction (RT-qPCR). The effect of miR-186-5p on WNT pathway was analysied with TOP/FOP flash assay in SW480 cell line. Finally, flow cytometery was used to inves tigate the effect of miR-186-5p overexpression on cell cycle progression in SW480 cell line. miR-186-5p was overexpressed in the SW480 cell line and its overexpression resulted in significant reduction of the TCF4 mRNA level. TOP/FOP flash assay, confirmed the negative effect of miR-186-5p on the Wnt pathway in SW480 cells. Finally, Overexpression of miR186-5p in SW480 cells resulted in cell cycle arrest in subG1 phase, detected by flow cytometry. Overall, accumulative results indi-cated that miR-186-5p by targeting TCF4 is potentially one of the regulators of the WNT signaling pathway.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5539/jmbr.v8n1p132","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47999742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. V. Ostroverkhova, Kucher An, N. Babushkina, O. L. Konusova, I. Sharakhov
An assessment of the genetic diversity of the microsatellite locus mrjp3 in honeybee of European Apis mellifera subspecies was conducted. Differences in the frequency of alleles for the mrjp3 locus were found in honeybees of different evolutionary branches. Allele "529" was found to be specific for the Apis mellifera mellifera, evolutionary branch M, while alleles "406", "518", and "485" were characteristic of the southern subspecies of bees (A. m. carnica, A. m. carpatica (some researchers consider this breed as a derivative of A. m. carnica), and A. m. caucasica; evolutionary branches C and O, respectively). A high correspondence of the studied nucleotide sequences (>=99% identity) with the reference sequences (Genbank) was established indicating a high conservation of the repetitive region of the mrjp3 gene in A. mellifera subspecies inhabiting different geographic regions of Europe and Siberia. Locus mrjp3 is of considerable interest for further study as a candidate marker for differentiation of bee subspecies.
对欧洲蜜蜂亚种微卫星位点mrjp3的遗传多样性进行了评价。在不同进化分支的蜜蜂中发现mrjp3位点的等位基因频率存在差异。等位基因“529”是蜜蜂进化分支“蜜蜂蜜蜂”所特有的，而等位基因“406”、“518”和“485”是南方亚种蜜蜂(a . M . carnica)、a . M . carpatica(一些研究者认为该品种是a . M . carnica的衍生物)和a . M . caucasica)所特有的;进化分支C和O分别)。所研究的核苷酸序列与参考序列(Genbank)高度对应(>=99%)，表明在生活在欧洲和西伯利亚不同地理区域的A. mellifera亚种中mrjp3基因的重复区域具有高度保守性。mrjp3基因座作为蜜蜂亚种分化的候选标记，值得进一步研究。
{"title":"Variability and Structure of the Repetitive Region of the Major Royal Jelly Protein Gene mrjp3 in Honeybee Apis mellifera of Different Evolutionary Branches","authors":"N. V. Ostroverkhova, Kucher An, N. Babushkina, O. L. Konusova, I. Sharakhov","doi":"10.5539/jmbr.v8n1p122","DOIUrl":"https://doi.org/10.5539/jmbr.v8n1p122","url":null,"abstract":"An assessment of the genetic diversity of the microsatellite locus mrjp3 in honeybee of European Apis mellifera subspecies was conducted. Differences in the frequency of alleles for the mrjp3 locus were found in honeybees of different evolutionary branches. Allele \"529\" was found to be specific for the Apis mellifera mellifera, evolutionary branch M, while alleles \"406\", \"518\", and \"485\" were characteristic of the southern subspecies of bees (A. m. carnica, A. m. carpatica (some researchers consider this breed as a derivative of A. m. carnica), and A. m. caucasica; evolutionary branches C and O, respectively). A high correspondence of the studied nucleotide sequences (>=99% identity) with the reference sequences (Genbank) was established indicating a high conservation of the repetitive region of the mrjp3 gene in A. mellifera subspecies inhabiting different geographic regions of Europe and Siberia. Locus mrjp3 is of considerable interest for further study as a candidate marker for differentiation of bee subspecies.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5539/jmbr.v8n1p122","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45563549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background and Objectives: Traumatic dental injuries (TDIs) are unpleasant experiences for children and they necessitate to be treated as soon as possible. This cross-sectional study aimed to assess the knowledge and practice of general dental practitioners (GDPs) regarding emergency management of TDIs in Ahvaz, Iran.Subjects and Methods: In this study, a two-part questionnaire was responded by 100 GDPs. The first section included questions on demographic information and the second section was composed of questions on different dental Injuries. One score was assigned to each correct answer; the total score of 10 to 30 was considered as low knowledge and practice, while scores 30-50, 50-70 and above 70 were considered as moderate, good, and high levels of knowledge and practice, respectively. The data were analyzed using Pearson’s Correlation, t-test and regression.Results: With regards to the level of GDP’s knowledge, the mean score was 59.2%. A total of 100 (51%) dentists showed a good level of knowledge. A significant association was found between knowledge and practice of GDPs in their practice encountering and treating TDI (P=0.001).Conclusion: The overall knowledge of GDPs about management of TDI in the selected community was good.
{"title":"Knowledge and Practice of General Dental Practitioners Concerning Dental Trauma Management in Children in Ahvaz, Iran","authors":"L. Basir, Mohsen Shayesteh, M. Heydari","doi":"10.5539/JMBR.V8N1P114","DOIUrl":"https://doi.org/10.5539/JMBR.V8N1P114","url":null,"abstract":"Background and Objectives: Traumatic dental injuries (TDIs) are unpleasant experiences for children and they necessitate to be treated as soon as possible. This cross-sectional study aimed to assess the knowledge and practice of general dental practitioners (GDPs) regarding emergency management of TDIs in Ahvaz, Iran.Subjects and Methods: In this study, a two-part questionnaire was responded by 100 GDPs. The first section included questions on demographic information and the second section was composed of questions on different dental Injuries. One score was assigned to each correct answer; the total score of 10 to 30 was considered as low knowledge and practice, while scores 30-50, 50-70 and above 70 were considered as moderate, good, and high levels of knowledge and practice, respectively. The data were analyzed using Pearson’s Correlation, t-test and regression.Results: With regards to the level of GDP’s knowledge, the mean score was 59.2%. A total of 100 (51%) dentists showed a good level of knowledge. A significant association was found between knowledge and practice of GDPs in their practice encountering and treating TDI (P=0.001).Conclusion: The overall knowledge of GDPs about management of TDI in the selected community was good.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5539/JMBR.V8N1P114","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41800774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Abdolsamadi, M. Vahedi, S. Borzouei, A. Soltanian, A. Hosseini, Meghdad Zakavati Avval
Introduction: The International Diabetes Federation (IDF) Announces 415 million people around the world are suffering from diabetes. There is a high proportion of UDM (undiagnosed diabetes) at the level of the world and particularly in developing countries. The number of people with UDM in Iran in 2015 are 2197.96 per 1000. Vitamin D3 deficiency is associated with increased risk of type 2 diabetes and for a long time, been recognized as a risk factor for glucose intolerance. Salivary composition, as the mirror of oral health, its use as a diagnostic tool is increasing and diabetes is also can be effective on the flow rate of saliva and its compounds. Analysis of saliva can be used as part of the evaluation of endocrine function. Material and Methods: In this case-control study in 2016 in Hamadan, we selected 57 newly diagnosed patients with type 2 diabetes mellitus with mean aged 47.73 years and 57 healthy controls with mean aged 45.36 years. By using Spitting method, 5ml of completely unstimulated saliva samples were collected from diabetic patients and control subjects. The serum and saliva 25(OH)Vit.Dconcentrations were measured by ELISA. The results are analyzed by SPSS 16.Results: Significant difference was found in serum concentrations between the two groups (p<0.001). But in unstimulated whole salivary 1, 25(OH) 2D3 concentrations between the two groups, difference was not significant and the relationship, was reversed. There was a significant correlation (P=0.013) between serum and saliva 1, 25(OH) 2D3concentrations in the control group. In other words, the correlation was approximately 0.33. Correlation between serum and saliva 1, 25(OH) 2D3 concentrations in case group was not significant. Serum levels of 1, 25(OH) 2D3 in 21.1% of control group, showed the inadequate level (Vit.D3= 20-29ng/ml) and in the case group, showed the critical situation and overall 91.2% of the newly diagnosed diabetics, suffered from lack of vitamin D3. According to the salivary levels of 1, 25(OH) 2D3, in general, a total of 58% of the case group, have deficiency of vitamin D3.Conclusion: The results was confirmed vitamin D3 deficiency in participating in the study population (both case and control). The findings showed that there is lower concentration of 1, 25(OH) 2D3 in serum and after that in saliva in diabetic patients that newly diagnosed. Saliva would play a helpful diagnostic role in the early detection, the monitoring and progression of diabetes, but still serum is the better method for detecting vit.D3 levels and more research needs to be done on saliva for detecting Vit.D3 concentrations.
{"title":"Evaluation the Relationship Between Serum and Salivary Levels of 25(OH)Vit.D with Type II Diabetes in Newly Diagnosed Diabetics","authors":"H. Abdolsamadi, M. Vahedi, S. Borzouei, A. Soltanian, A. Hosseini, Meghdad Zakavati Avval","doi":"10.5539/JMBR.V8N1P108","DOIUrl":"https://doi.org/10.5539/JMBR.V8N1P108","url":null,"abstract":"Introduction: The International Diabetes Federation (IDF) Announces 415 million people around the world are suffering from diabetes. There is a high proportion of UDM (undiagnosed diabetes) at the level of the world and particularly in developing countries. The number of people with UDM in Iran in 2015 are 2197.96 per 1000. Vitamin D3 deficiency is associated with increased risk of type 2 diabetes and for a long time, been recognized as a risk factor for glucose intolerance. Salivary composition, as the mirror of oral health, its use as a diagnostic tool is increasing and diabetes is also can be effective on the flow rate of saliva and its compounds. Analysis of saliva can be used as part of the evaluation of endocrine function. Material and Methods: In this case-control study in 2016 in Hamadan, we selected 57 newly diagnosed patients with type 2 diabetes mellitus with mean aged 47.73 years and 57 healthy controls with mean aged 45.36 years. By using Spitting method, 5ml of completely unstimulated saliva samples were collected from diabetic patients and control subjects. The serum and saliva 25(OH)Vit.Dconcentrations were measured by ELISA. The results are analyzed by SPSS 16.Results: Significant difference was found in serum concentrations between the two groups (p<0.001). But in unstimulated whole salivary 1, 25(OH) 2D3 concentrations between the two groups, difference was not significant and the relationship, was reversed. There was a significant correlation (P=0.013) between serum and saliva 1, 25(OH) 2D3concentrations in the control group. In other words, the correlation was approximately 0.33. Correlation between serum and saliva 1, 25(OH) 2D3 concentrations in case group was not significant. Serum levels of 1, 25(OH) 2D3 in 21.1% of control group, showed the inadequate level (Vit.D3= 20-29ng/ml) and in the case group, showed the critical situation and overall 91.2% of the newly diagnosed diabetics, suffered from lack of vitamin D3. According to the salivary levels of 1, 25(OH) 2D3, in general, a total of 58% of the case group, have deficiency of vitamin D3.Conclusion: The results was confirmed vitamin D3 deficiency in participating in the study population (both case and control). The findings showed that there is lower concentration of 1, 25(OH) 2D3 in serum and after that in saliva in diabetic patients that newly diagnosed. Saliva would play a helpful diagnostic role in the early detection, the monitoring and progression of diabetes, but still serum is the better method for detecting vit.D3 levels and more research needs to be done on saliva for detecting Vit.D3 concentrations.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5539/JMBR.V8N1P108","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41867569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Abdolsamadi, M. Vahedi, F. Fariba, A. Soltanian, Meghdad Zakavati Avval, A. Hosseini
Introduction: Vitamin D deficiency is a major public health problem. Low vitamin D levels associated with adverse health consequences such as musculoskeletal health, cognitive decline and progression of cancer and death. The lack of vitamin D associated with major risk factors for cardiovascular disease (CVD) includes hypertension is considered. The ability to assess the general health, disease and treatment outcomes through saliva as a non-invasive, inexpensive and simple method of interest is located. The aim of this study was a comparative study of salivary and serum levels of vitamin D3 in patients with a history of developing high blood pressure and a healthy person.Methods: This study was a case - control survey, in which 40 patients with high blood pressure were examined. The control group including 40 healthy subjects. Both groups were matched in terms of age and gender. After collecting samples of serum and saliva, the amount of vitamin D level samples were measured using ELISA method by electrochemiluminescence (ELC), and then analyzed the results using software SPSS 16 and statistical test including Chi Square Test, Independent-Samples, linear regression model, the Mann-Whitney Test and Spearman correlation coefficient.Results: There was no significant difference in the mean serum levels of vitamin D among patients and healthy subjects (p= 0.588). In addition, there was no significant difference in the mean salivary levels of vitamin D between patients and healthy subjects (p= 0.833). There was no significant relationship between salivary and serum level of vitamin D in healthy individuals (p= 0.095). As well as there was no significant correlation between salivary and serum level of vitamin D in patients (p= 0.5).Conclusions: This study showed that vitamin D is a measurable marker in saliva, but its analysis in saliva, may not be a reliable tool for determining the vitamin D levels.
{"title":"Comparative Study of Salivary and Serum Levels of Vitamin D in Patients with a History of High Blood Pressure and Healthy People","authors":"H. Abdolsamadi, M. Vahedi, F. Fariba, A. Soltanian, Meghdad Zakavati Avval, A. Hosseini","doi":"10.5539/JMBR.V8N1P101","DOIUrl":"https://doi.org/10.5539/JMBR.V8N1P101","url":null,"abstract":"Introduction: Vitamin D deficiency is a major public health problem. Low vitamin D levels associated with adverse health consequences such as musculoskeletal health, cognitive decline and progression of cancer and death. The lack of vitamin D associated with major risk factors for cardiovascular disease (CVD) includes hypertension is considered. The ability to assess the general health, disease and treatment outcomes through saliva as a non-invasive, inexpensive and simple method of interest is located. The aim of this study was a comparative study of salivary and serum levels of vitamin D3 in patients with a history of developing high blood pressure and a healthy person.Methods: This study was a case - control survey, in which 40 patients with high blood pressure were examined. The control group including 40 healthy subjects. Both groups were matched in terms of age and gender. After collecting samples of serum and saliva, the amount of vitamin D level samples were measured using ELISA method by electrochemiluminescence (ELC), and then analyzed the results using software SPSS 16 and statistical test including Chi Square Test, Independent-Samples, linear regression model, the Mann-Whitney Test and Spearman correlation coefficient.Results: There was no significant difference in the mean serum levels of vitamin D among patients and healthy subjects (p= 0.588). In addition, there was no significant difference in the mean salivary levels of vitamin D between patients and healthy subjects (p= 0.833). There was no significant relationship between salivary and serum level of vitamin D in healthy individuals (p= 0.095). As well as there was no significant correlation between salivary and serum level of vitamin D in patients (p= 0.5).Conclusions: This study showed that vitamin D is a measurable marker in saliva, but its analysis in saliva, may not be a reliable tool for determining the vitamin D levels.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5539/JMBR.V8N1P101","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45431474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Falahi, K. Gazvini, M. Mirzaei, Araz Majnoni, H. Lotfi, S. Jamehdar
Background: Mycobacterium tuberculosis is an infectious agent responsible for major health problems and a large number of mortalities. The prevalence of Mycobacterium tuberculosis infection varies across countries. Knowing the infection prevalence can aid in improving public health and reduce the associated costs. The aim of this study was to determine the prevalence of tuberculosis (TB) infection in suspected cases in Mashhad, Iran.Methods: All the clinical specimens suspected of TB infection were sent to a laboratory for diagnosis during -March 2017 to March 2018. The samples were analyzed microscopically using Ziehl–Neelsen staining, by polymerase chain reaction (PCR) to identify the Mycobacterium tuberculosis species using IS6110 primers, and the samples were also grown on Lowenstein–Jensen medium.Results: Of 2,755 clinical samples analyzed, 153 (5.55%) were identified as Mycobacterium tuberculosis-positive, of which 54.9% originated from females and 45.1% from males. The highest rate of infection was observed in spring, especially in May (15%). Most TB cases were found in patients in VIP (43.1%), thorax (17%), and internal (15%) wards. TB infection was mostly detected in bronchial tube (70%) and sputum (23.5%) samples. The most common positive smear was 1+ (36%). Of the 153 cases, (147) 96.1% were culture –positive and 2% were PCR-negative also 84.3% were smear –positive.Conclusion: The highest rate of infection occurred in spring, when the number of religious tourists entering the city was at its peak. Considering the sensitive location of this city, awareness regarding TB status can lead to improved health in the community and development of basic strategies to control and eliminate the transmission of this infection from Mashhad to other areas.
{"title":"Prevalence of Mycobacterium tuberculosis Infection in Suspected Patients in a Teaching Hospital in Northeastern Iran: A Cross-Sectional Study","authors":"J. Falahi, K. Gazvini, M. Mirzaei, Araz Majnoni, H. Lotfi, S. Jamehdar","doi":"10.5539/JMBR.V8N1P95","DOIUrl":"https://doi.org/10.5539/JMBR.V8N1P95","url":null,"abstract":"Background: Mycobacterium tuberculosis is an infectious agent responsible for major health problems and a large number of mortalities. The prevalence of Mycobacterium tuberculosis infection varies across countries. Knowing the infection prevalence can aid in improving public health and reduce the associated costs. The aim of this study was to determine the prevalence of tuberculosis (TB) infection in suspected cases in Mashhad, Iran.Methods: All the clinical specimens suspected of TB infection were sent to a laboratory for diagnosis during -March 2017 to March 2018. The samples were analyzed microscopically using Ziehl–Neelsen staining, by polymerase chain reaction (PCR) to identify the Mycobacterium tuberculosis species using IS6110 primers, and the samples were also grown on Lowenstein–Jensen medium.Results: Of 2,755 clinical samples analyzed, 153 (5.55%) were identified as Mycobacterium tuberculosis-positive, of which 54.9% originated from females and 45.1% from males. The highest rate of infection was observed in spring, especially in May (15%). Most TB cases were found in patients in VIP (43.1%), thorax (17%), and internal (15%) wards. TB infection was mostly detected in bronchial tube (70%) and sputum (23.5%) samples. The most common positive smear was 1+ (36%). Of the 153 cases, (147) 96.1% were culture –positive and 2% were PCR-negative also 84.3% were smear –positive.Conclusion: The highest rate of infection occurred in spring, when the number of religious tourists entering the city was at its peak. Considering the sensitive location of this city, awareness regarding TB status can lead to improved health in the community and development of basic strategies to control and eliminate the transmission of this infection from Mashhad to other areas.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5539/JMBR.V8N1P95","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42966605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Bagheri, M. Shooriabi, M. Amin, Fatemeh Babadi, A. Ahmadzadeh, M. Sabet, H. Mozaffari
Introduction: Performing dental practices in the oral environment leads to the transmission of microorganisms in saliva and blood to working surfaces and dental devices and their infection. Preventing transmission of infection through these devices is an important task of a dentist. Hence, this research was conducted to evaluate the disinfection quality of the dental faculty units of Ahvaz Jundishapur University of Medical Sciences (AJUMS) in Iran.Materials and Methods: In order to evaluate the quality of disinfection of the units, sampling was performed from all glasses spittoon surfaces of 90 units of the clinical unit of the AJUMS dental faculty before and after disinfection by personnel. Then, the bacteria were cultured in a medium and examined.Results: The mean (and standard deviation) of the total infection of units of the dental faculty was 46534.4 (583380.4) colonies per 1 ml before disinfection and 40265.6 (52131.1) colonies per 1 ml after disinfection, reflecting significant decrease in number of bacterial colonies after disinfection (P <0.001). In addition, a significant decrease was seen in the number of bacterial colonies in the restoration, pediatric, orthodontic and diagnosis units (p <0.05), but the difference before and after disinfection was not significant in the prosthetic, endodontic, surgical and periodontal units. In addition, the most common types of microorganisms in the whole units of the dental faculty were pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus, respectively, and after disinfection, the most common types of microorganisms were Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus, respectively.Conclusion: In general, this study showed that the disinfection method of units in dental faculty can not reduce the severity of infection of the units. Given what was stated, it is recommended that the method and the substances used to disinfect the unit to be changed.
{"title":"Evaluation of Disinfection Quality of Dental Faculty Units of Ahvaz Jundishapur University of Medical Sciences, Southwest of Iran in 2017","authors":"S. Bagheri, M. Shooriabi, M. Amin, Fatemeh Babadi, A. Ahmadzadeh, M. Sabet, H. Mozaffari","doi":"10.5539/JMBR.V8N1P88","DOIUrl":"https://doi.org/10.5539/JMBR.V8N1P88","url":null,"abstract":"Introduction: Performing dental practices in the oral environment leads to the transmission of microorganisms in saliva and blood to working surfaces and dental devices and their infection. Preventing transmission of infection through these devices is an important task of a dentist. Hence, this research was conducted to evaluate the disinfection quality of the dental faculty units of Ahvaz Jundishapur University of Medical Sciences (AJUMS) in Iran.Materials and Methods: In order to evaluate the quality of disinfection of the units, sampling was performed from all glasses spittoon surfaces of 90 units of the clinical unit of the AJUMS dental faculty before and after disinfection by personnel. Then, the bacteria were cultured in a medium and examined.Results: The mean (and standard deviation) of the total infection of units of the dental faculty was 46534.4 (583380.4) colonies per 1 ml before disinfection and 40265.6 (52131.1) colonies per 1 ml after disinfection, reflecting significant decrease in number of bacterial colonies after disinfection (P <0.001). In addition, a significant decrease was seen in the number of bacterial colonies in the restoration, pediatric, orthodontic and diagnosis units (p <0.05), but the difference before and after disinfection was not significant in the prosthetic, endodontic, surgical and periodontal units. In addition, the most common types of microorganisms in the whole units of the dental faculty were pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus, respectively, and after disinfection, the most common types of microorganisms were Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus, respectively.Conclusion: In general, this study showed that the disinfection method of units in dental faculty can not reduce the severity of infection of the units. Given what was stated, it is recommended that the method and the substances used to disinfect the unit to be changed.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5539/JMBR.V8N1P88","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45524606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：