Pub Date : 2022-10-01DOI: 10.15407/biotech15.05.005
I. Kosakivska
Background. Aquatic ecosystems are subjected to significant stress loads and depletion due to the influx of pollutants of inorganic and organic origin, that pose a serious threat to human health. The United Nations Environment Program has defined phytoremediation as an effective eco-technology for the removal, detoxification and immobilization of pollutants using plants. Water ferns of the Salviniaceae family belong to promising phytoremediants. They are characterized by high growth rates, resistance to adverse environmental factors, capable of adsorbing pollutants, including heavy metals. Species of the genus Salvinia and Azolla are used to assess the ecological state of water and study ecotoxicological effects of pollutants. Aim. Analysis and generalization of the latest scientific results on the use of species of the Salviniaceae family for phytoremediation and phytoindication of contaminated water. Results. In this review, we have highlighted key information on emerging phytotechnologies, including phytodegradation, phytostabilization, rhizofiltration, rhizodegradation, and phytovolatization. The growth and distribution features of species of the genus Salvinia and Azolla were described and current information on the use of water ferns for cleaning polluted water from heavy metals, inorganic and organic pollutants was presented. Data on the physiological and molecular mechanisms of the genus Salvinia and Azolla species adaptation to the toxic effect of pollutants of various origins were discussed. We focused special attention on the use of water ferns of the Salviniaceae family to control water pollution.
{"title":"WATER FERNS OF Salviniaceae FAMILY IN PHYTOREMEDIATION AND PHYTOINDICATION OF CONTAMINATED WATER","authors":"I. Kosakivska","doi":"10.15407/biotech15.05.005","DOIUrl":"https://doi.org/10.15407/biotech15.05.005","url":null,"abstract":"Background. Aquatic ecosystems are subjected to significant stress loads and depletion due to the influx of pollutants of inorganic and organic origin, that pose a serious threat to human health. The United Nations Environment Program has defined phytoremediation as an effective eco-technology for the removal, detoxification and immobilization of pollutants using plants. Water ferns of the Salviniaceae family belong to promising phytoremediants. They are characterized by high growth rates, resistance to adverse environmental factors, capable of adsorbing pollutants, including heavy metals. Species of the genus Salvinia and Azolla are used to assess the ecological state of water and study ecotoxicological effects of pollutants. Aim. Analysis and generalization of the latest scientific results on the use of species of the Salviniaceae family for phytoremediation and phytoindication of contaminated water. Results. In this review, we have highlighted key information on emerging phytotechnologies, including phytodegradation, phytostabilization, rhizofiltration, rhizodegradation, and phytovolatization. The growth and distribution features of species of the genus Salvinia and Azolla were described and current information on the use of water ferns for cleaning polluted water from heavy metals, inorganic and organic pollutants was presented. Data on the physiological and molecular mechanisms of the genus Salvinia and Azolla species adaptation to the toxic effect of pollutants of various origins were discussed. We focused special attention on the use of water ferns of the Salviniaceae family to control water pollution.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48430292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-31DOI: 10.15407/biotech15.04.027
D. Ostapchenko
Aim. This study was aimed to examine influence of сytokinin fraction of basidiomycete Hericium coralloides on the spontaneous and induced phagocytic activity of murine peritoneal macrophages. Materials and methods. Mononuclear phagocyte (macrophage) fraction of peritoneal exudate of C57BL/6 mice was used. Macrophages were incubated under standard conditions at 37 °C, 100% humidity and 5% CO2 for 4 hours. Phorbol 12-myristate 13-acetate (PMA) was added to part of the samples to activate oxidative metabolism. Hericium coralloides was added in two concentrations that were 5 and 10 times lower than the IC50, defined as an antiproliferative effect on colon cancer cells. Incubation with samples was carried out for 2 hours. Results. When adding PMA, Hericium coralloides (0.017 and 0.035 μg/ml) and in the combination of PMA with Hericium coralloides, activation of reactive oxygen species (ROS) in peritoneal macrophages was revealed by 1.37-1.7 times, compared to the spontaneous activity of phagocytes. Conclusions. Thus, the effect of the cytokinin e.tract of the basidium fungus Hericium coralloides was manifested by an increase in the phagocytic activity of peritoneal macrophages as one of the possible mechanisms of immunomodulatory action.
{"title":"CYTOKININ FRACTION OF THE Hericium coralloides INCREASES OXIDATIVE METABOLISM OF MURINE PERITONEAL MACROPHAGIES","authors":"D. Ostapchenko","doi":"10.15407/biotech15.04.027","DOIUrl":"https://doi.org/10.15407/biotech15.04.027","url":null,"abstract":"Aim. This study was aimed to examine influence of сytokinin fraction of basidiomycete Hericium coralloides on the spontaneous and induced phagocytic activity of murine peritoneal macrophages. Materials and methods. Mononuclear phagocyte (macrophage) fraction of peritoneal exudate of C57BL/6 mice was used. Macrophages were incubated under standard conditions at 37 °C, 100% humidity and 5% CO2 for 4 hours. Phorbol 12-myristate 13-acetate (PMA) was added to part of the samples to activate oxidative metabolism. Hericium coralloides was added in two concentrations that were 5 and 10 times lower than the IC50, defined as an antiproliferative effect on colon cancer cells. Incubation with samples was carried out for 2 hours. Results. When adding PMA, Hericium coralloides (0.017 and 0.035 μg/ml) and in the combination of PMA with Hericium coralloides, activation of reactive oxygen species (ROS) in peritoneal macrophages was revealed by 1.37-1.7 times, compared to the spontaneous activity of phagocytes. Conclusions. Thus, the effect of the cytokinin e.tract of the basidium fungus Hericium coralloides was manifested by an increase in the phagocytic activity of peritoneal macrophages as one of the possible mechanisms of immunomodulatory action.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41464242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-31DOI: 10.15407/biotech15.04.037
Ya.S. Kulyk
Background. Better and high-potency vaccines against diphtheria are urgently needed to provide broader protection against diverse strains and subtypes. Identification of novel broadly neutralizing epitopes targeted by protective antibodies could aid in such efforts. Aim. In this study we focused on the search of binding sites identification of anti diphtheria toxin monoclonal antibodies and their neutralizing activity to block binding of recombinant exotoxin derivates with host receptors. Methods. Vero cells were cultured in the complete RPMI-1640 medium under standard conditions and used for flow cytometry assay. Recombinant antigens and products of tryptic hydrolysis of CRM197 and SbB were characterized by Ni2+-NTA affinity chromatography and SDS-PAGE under reducing conditions with following ECL Western-Blot using several hybridomas clones of anti-diphtheria toxin monoclonal antibodies. Results. ECL western blot film results for clone 9.1-E11 showed the specific binding both to whole CRM197 molecule, and to almost all fragments of CRM197 formed as a result of limited proteolysis. In particular, a band corresponding to SbB in molecular weight can be identified. Thus, epitope region of the CRM197 molecule specific to 9.1-E1 mAbs is located within the structure of SbB. At the same time 16.4-E9 clone antibodies had high specificity to R-domain of SbB. In addition, both hybridoma clones antibodies have neutralizing activity against the DT binding subunit, which is a key factor in blocking between cell receptor and it ligand, C.diphtheriae exotoxin. Conclusions. The results obtained indicate that obtained antibodies are prospective for improving new diagnostic tools and therapeutic agents, which are used for treatment and understanding of the molecular mechanisms of diphtheria pathogenesis.
背景。迫切需要更好和高效的白喉疫苗，以提供针对不同毒株和亚型的更广泛保护。鉴定保护性抗体靶向的新的广泛中和表位可以帮助实现这一目标。的目标。在本研究中，我们主要研究了抗白喉毒素单克隆抗体的结合位点鉴定及其阻断重组外毒素衍生物与宿主受体结合的中和活性。方法。Vero细胞在标准条件下于完整的RPMI-1640培养基中培养，并用于流式细胞术检测。利用几种抗白喉毒素单克隆抗体杂交瘤克隆，采用Ni2+-NTA亲和层析和SDS-PAGE在还原条件下对CRM197和SbB的重组抗原和产物进行了表征。结果。克隆9.1-E11的ECL western blot结果显示，该蛋白既能与CRM197的整个分子结合，又能与由于有限蛋白水解而形成的几乎所有CRM197片段结合。特别是，可以识别出分子量与SbB相对应的条带。因此，CRM197分子对9.1-E1单克隆抗体特异性的表位区域位于SbB结构内。同时，16.4-E9克隆抗体对SbB的r结构域具有较高的特异性。此外，这两种杂交瘤克隆抗体对DT结合亚基具有中和活性，DT结合亚基是阻断细胞受体与其配体白喉白喉外毒素的关键因素。结论。结果表明，所获得的抗体有望开发新的诊断工具和治疗剂，用于治疗和了解白喉发病机制的分子机制。
{"title":"EPITOPES IDENTIFICATION OF BROADLY NEUTRALIZING MONOCLONAL ANTIBODIES AGAINST Corynebacterium diphtheriae EXOTOXIN","authors":"Ya.S. Kulyk","doi":"10.15407/biotech15.04.037","DOIUrl":"https://doi.org/10.15407/biotech15.04.037","url":null,"abstract":"Background. Better and high-potency vaccines against diphtheria are urgently needed to provide broader protection against diverse strains and subtypes. Identification of novel broadly neutralizing epitopes targeted by protective antibodies could aid in such efforts. Aim. In this study we focused on the search of binding sites identification of anti diphtheria toxin monoclonal antibodies and their neutralizing activity to block binding of recombinant exotoxin derivates with host receptors. Methods. Vero cells were cultured in the complete RPMI-1640 medium under standard conditions and used for flow cytometry assay. Recombinant antigens and products of tryptic hydrolysis of CRM197 and SbB were characterized by Ni2+-NTA affinity chromatography and SDS-PAGE under reducing conditions with following ECL Western-Blot using several hybridomas clones of anti-diphtheria toxin monoclonal antibodies. Results. ECL western blot film results for clone 9.1-E11 showed the specific binding both to whole CRM197 molecule, and to almost all fragments of CRM197 formed as a result of limited proteolysis. In particular, a band corresponding to SbB in molecular weight can be identified. Thus, epitope region of the CRM197 molecule specific to 9.1-E1 mAbs is located within the structure of SbB. At the same time 16.4-E9 clone antibodies had high specificity to R-domain of SbB. In addition, both hybridoma clones antibodies have neutralizing activity against the DT binding subunit, which is a key factor in blocking between cell receptor and it ligand, C.diphtheriae exotoxin. Conclusions. The results obtained indicate that obtained antibodies are prospective for improving new diagnostic tools and therapeutic agents, which are used for treatment and understanding of the molecular mechanisms of diphtheria pathogenesis.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44392404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-31DOI: 10.15407/biotech15.04.018
A. Nefodova
Aim. The purpose of the study was to evaluate manifestations of systemic inflammation in rats with Alzheimer disease (AD) induced by injections of Aβ1-40 and Aβ25-35 by the assessment of functional polarization of circulating phagocytes. Methods. AD was induced by intracerebral injections of Aβ1–40 and Aβ25–35 Wistar male rats. Intact and sham-operated animals were used as a control. AD development was affirmed by the assessment of cognitive impairment in behavioral tests (‘Open field’ test, apomorphine test, Barnes maze test), as well as by the level of death of dopaminergic neurons. The functional polarization of circulating phagocytes was designated by phagocytic activity, oxidative metabolism, and the expression of phenotypic markers CD80 and CD206, which were examined by flow cytometry. Results. Circulating phagocytes from rats with Aβ1-40-induced AD were characterized by increased fraction of phagocytizing monocytes with decreased endocytic activity, moderately up-regulated granulocyte ROS generation along with temperate increase of CD86+ mononuclear phagocyte fraction and high level of CD206 expression. Two widely accepted indices of systemic inflammation: NLR and SII were higher in these animals than those in control rats by 6,5 and 7,5 times respectively. In rats with Aβ25-35-induced disease, significantly increased granulocyte ROS generation was registered. NLR and SII values in these animals were slightly higher than those in control rats. Conclusion. Therefore, Aβ1-40 AD model reproduces disease-associated systemic inflammation at the greater extent than Aβ25-35-induced pathology, and is more appropriate for the study of inflammation in the disease pathophysiology.
{"title":"FUNCTIONAL AND PHENOTYPIC CHARACTERISTICS OF CIRCULATING PHAGOCYTES IN RATS WITH DIFFERENT MODELS OF ALZHEIMER’S DISEASE","authors":"A. Nefodova","doi":"10.15407/biotech15.04.018","DOIUrl":"https://doi.org/10.15407/biotech15.04.018","url":null,"abstract":"Aim. The purpose of the study was to evaluate manifestations of systemic inflammation in rats with Alzheimer disease (AD) induced by injections of Aβ1-40 and Aβ25-35 by the assessment of functional polarization of circulating phagocytes. Methods. AD was induced by intracerebral injections of Aβ1–40 and Aβ25–35 Wistar male rats. Intact and sham-operated animals were used as a control. AD development was affirmed by the assessment of cognitive impairment in behavioral tests (‘Open field’ test, apomorphine test, Barnes maze test), as well as by the level of death of dopaminergic neurons. The functional polarization of circulating phagocytes was designated by phagocytic activity, oxidative metabolism, and the expression of phenotypic markers CD80 and CD206, which were examined by flow cytometry. Results. Circulating phagocytes from rats with Aβ1-40-induced AD were characterized by increased fraction of phagocytizing monocytes with decreased endocytic activity, moderately up-regulated granulocyte ROS generation along with temperate increase of CD86+ mononuclear phagocyte fraction and high level of CD206 expression. Two widely accepted indices of systemic inflammation: NLR and SII were higher in these animals than those in control rats by 6,5 and 7,5 times respectively. In rats with Aβ25-35-induced disease, significantly increased granulocyte ROS generation was registered. NLR and SII values in these animals were slightly higher than those in control rats. Conclusion. Therefore, Aβ1-40 AD model reproduces disease-associated systemic inflammation at the greater extent than Aβ25-35-induced pathology, and is more appropriate for the study of inflammation in the disease pathophysiology.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49638412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-31DOI: 10.15407/biotech15.04.008
R. Dovhyi
Aim. This study aimed to investigate the circadian rhythm of the murine peritoneal macrophage (PM) metabolic profile. Methods. The metabolic profile of PM was characterized by phagocytic activity, reactive oxygen species (ROS) generation, and by the expression of phenotypic markers, associated with a pro- and anti-inflammatory metabolic shift. Phagocytosis of FITC-labeled inactivated Staphylococcus aureus, ROS generation, CD80, CD86, and CD206 expression were estimated by flow cytometry at a regular 4h interval over the daily light-dark cycle. Results. The phagocytic index and percentage of ROS-producing PM were found to be lower in the resting phase (ZT4) as compared to the active phase. In contrast, the level of CD86 expression was the highest in the inactive phase (ZT8). There was also a statistically significant peak in the proportion of ROS-producing PM, as well as in the level of ROS production per cell at the time of awakening (ZT12). As opposed to ROS generation, ZT12 was characterized by the lowest level of cell-surface CD206 expression. Conclusions. Our results indicate that there is a circadian rhythm in functional polarization of murine PM with an anti-inflammatory activation state in the resting phase in comparison to the active phase.
{"title":"CIRCADIAN VARIATION IN FUNCTIONAL POLARIZATION OF MURINE PERITONEAL MACROPHAGES","authors":"R. Dovhyi","doi":"10.15407/biotech15.04.008","DOIUrl":"https://doi.org/10.15407/biotech15.04.008","url":null,"abstract":"Aim. This study aimed to investigate the circadian rhythm of the murine peritoneal macrophage (PM) metabolic profile. Methods. The metabolic profile of PM was characterized by phagocytic activity, reactive oxygen species (ROS) generation, and by the expression of phenotypic markers, associated with a pro- and anti-inflammatory metabolic shift. Phagocytosis of FITC-labeled inactivated Staphylococcus aureus, ROS generation, CD80, CD86, and CD206 expression were estimated by flow cytometry at a regular 4h interval over the daily light-dark cycle. Results. The phagocytic index and percentage of ROS-producing PM were found to be lower in the resting phase (ZT4) as compared to the active phase. In contrast, the level of CD86 expression was the highest in the inactive phase (ZT8). There was also a statistically significant peak in the proportion of ROS-producing PM, as well as in the level of ROS production per cell at the time of awakening (ZT12). As opposed to ROS generation, ZT12 was characterized by the lowest level of cell-surface CD206 expression. Conclusions. Our results indicate that there is a circadian rhythm in functional polarization of murine PM with an anti-inflammatory activation state in the resting phase in comparison to the active phase.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47139576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-31DOI: 10.15407/biotech15.04.015
I.G. Kolobova
Aim. The purpose of the study was to examine beneficial effect of conditioned media (CM) of progenitor cells of different origin (neurogenic progenitor cells, or NPCs, and adipose-derived mononuclear cells, or AMCs) as a source of mesenchymal multipotent stromal cells (MMSCs) on brain cortex neurons in rats with traumatic brain injury (TBI). Methods. TBI was reproduced in outbred sexually mature male rats by developing the model of free-falling load (50 g) with damage to the left hemisphere of the brain. The rats were injected 3 times with an interval of every other day intraperitoneally with NPCs CM and AMCs CM (dose 0.8 mg by total protein) that were obtained from cell cultures of fetal rat brain and adult rat adipose tissue. On the 5th day after TBI, the morphologic study of brain tissue was performed. Results. The number of neurons in the cortex of rats on the 5th day after TBI in damaged hemisphere as well as in contralateral hemisphere compared to control group decreased by half. Three i.p. injections of NPCs CM or AMCs CM increased the number of neurons in the cortex in both hemispheres in rats of corresponding groups compared to the rats with TBI without additional treatment. Conclusion. Obtained results indicate that CM obtained from NPCs and AMCs have noticeable neuroprotective effect on the damaged neurons and might be considered as an additional mode to treatment of TBI.
{"title":"EFFECTS OF PROGENITOR CELL CONDITIONED MEDIA ON THE AMOUNT OF BRAIN CORTEX NEURONS IN A RAT MODEL OF TRAUMATIC BRAIN INJURY","authors":"I.G. Kolobova","doi":"10.15407/biotech15.04.015","DOIUrl":"https://doi.org/10.15407/biotech15.04.015","url":null,"abstract":"Aim. The purpose of the study was to examine beneficial effect of conditioned media (CM) of progenitor cells of different origin (neurogenic progenitor cells, or NPCs, and adipose-derived mononuclear cells, or AMCs) as a source of mesenchymal multipotent stromal cells (MMSCs) on brain cortex neurons in rats with traumatic brain injury (TBI). Methods. TBI was reproduced in outbred sexually mature male rats by developing the model of free-falling load (50 g) with damage to the left hemisphere of the brain. The rats were injected 3 times with an interval of every other day intraperitoneally with NPCs CM and AMCs CM (dose 0.8 mg by total protein) that were obtained from cell cultures of fetal rat brain and adult rat adipose tissue. On the 5th day after TBI, the morphologic study of brain tissue was performed. Results. The number of neurons in the cortex of rats on the 5th day after TBI in damaged hemisphere as well as in contralateral hemisphere compared to control group decreased by half. Three i.p. injections of NPCs CM or AMCs CM increased the number of neurons in the cortex in both hemispheres in rats of corresponding groups compared to the rats with TBI without additional treatment. Conclusion. Obtained results indicate that CM obtained from NPCs and AMCs have noticeable neuroprotective effect on the damaged neurons and might be considered as an additional mode to treatment of TBI.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47934583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-31DOI: 10.15407/biotech15.04.005
I.O. Bortyanuy
Successful colonization of Antarctic lands by vascular plants Deschampsia antarctica and Colobanthus quitensis and their adaptation to stressful environments is associated not only with climate change but also with the functioning of microbial groups of phylo- and endosphere of these plants. The aim of our study was to screen plant growth-promoting traits in endophytic bacteria of antarctic vascular plants. Materials and methods. We have studied 8 bacterial cultures isolated from D. antarctica collected during the 25th Ukrainian Antarctic Expedition (January-April 2020) along the Western part of the Antarctic Peninsula. Overnight liquid cultures were obtained on Nutrient Broth medium (HiMedia, Ltd.) in a shaking incubator (26 ℃, 160 rpm). Bacterial isolates were grown on Ashby's combined-nitrogen-free medium with sucrose. Drop collapse assay for cyclic lipopeptide production (CLP), motility assay, exoprotease production and phosphate solubilizing ability were performed using generally accepted methods. Results. All studied isolates have shown plant growth-promoting traits. The most abundant were nitrogen-fixing activity and motility. Both these play important role in plant colonization and promoting the growth of plants in harsh environments. The evidences of CLP were shown by two strains only. There was no notice of phosphate solubilizing ability and exoprotease production. Conclusions. Endophytic bacteria of antarctic vascular plants could support the growth and nutrition needs of the plants.
维管束植物南极Deschampsia and Colobanthus quitensis在南极陆地上的成功定殖及其对逆境环境的适应不仅与气候变化有关，而且与这些植物的门层和内圈微生物群的功能有关。本研究的目的是筛选南极维管植物内生细菌的植物促生性状。材料和方法。我们研究了在南极半岛西部第25次乌克兰南极考察(2020年1月至4月)期间收集的8种细菌培养物。在营养肉汤培养基(HiMedia, Ltd)的摇箱(26℃，160 rpm)中获得隔夜液体培养。分离的细菌在含蔗糖的Ashby无氮联合培养基上生长。采用普遍接受的方法进行了环脂肽生成(CLP)、活力测定、外蛋白酶生成和磷酸盐溶解能力的跌落试验。结果。所有研究的分离株都显示出促进植物生长的性状。最丰富的是固氮活性和运动性。这两种物质在植物定殖和促进植物在恶劣环境下的生长中起着重要作用。只有两株菌株显示了CLP的证据。没有注意到磷酸盐的溶解能力和外蛋白酶的产生。结论。南极维管植物的内生细菌能够支持植物的生长和营养需求。
{"title":"PLANT GROWTH-PROMOTING TRAITS OF ANTARCTIC ENDOPHYTIC BACTERIA","authors":"I.O. Bortyanuy","doi":"10.15407/biotech15.04.005","DOIUrl":"https://doi.org/10.15407/biotech15.04.005","url":null,"abstract":"Successful colonization of Antarctic lands by vascular plants Deschampsia antarctica and Colobanthus quitensis and their adaptation to stressful environments is associated not only with climate change but also with the functioning of microbial groups of phylo- and endosphere of these plants. The aim of our study was to screen plant growth-promoting traits in endophytic bacteria of antarctic vascular plants. Materials and methods. We have studied 8 bacterial cultures isolated from D. antarctica collected during the 25th Ukrainian Antarctic Expedition (January-April 2020) along the Western part of the Antarctic Peninsula. Overnight liquid cultures were obtained on Nutrient Broth medium (HiMedia, Ltd.) in a shaking incubator (26 ℃, 160 rpm). Bacterial isolates were grown on Ashby's combined-nitrogen-free medium with sucrose. Drop collapse assay for cyclic lipopeptide production (CLP), motility assay, exoprotease production and phosphate solubilizing ability were performed using generally accepted methods. Results. All studied isolates have shown plant growth-promoting traits. The most abundant were nitrogen-fixing activity and motility. Both these play important role in plant colonization and promoting the growth of plants in harsh environments. The evidences of CLP were shown by two strains only. There was no notice of phosphate solubilizing ability and exoprotease production. Conclusions. Endophytic bacteria of antarctic vascular plants could support the growth and nutrition needs of the plants.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43283737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-31DOI: 10.15407/biotech15.04.041
V. Sokolenko
Aim. The analysis of the indicators of skin microbiota and phagocytic activity of neutrophils and monocytes in employees of the "Peremoga Nova" poultry farm. Methods. The presence of sanitary and epidemiologically important groups of bacteria and the number of mesophilic aerobic and facultative anaerobic microorganisms (MAFAnM) on the skin surface, leukogram parameters and phagocytic activity of professional phagocytes were determined. The indicators of students of the Bohdan Khmelnytsky National University of Cherkasy were used as a control. Results. It was found that the MAFAnM index in the experimental group (3.2×103 CFU/cm3) is significantly lower than in the control group (2.7×103 CFU/cm3), however, the percentage of Staphylococcus spp. carriers is higher (67.5% versus 40.0%). In the experimental group, the relative and total number of monocytes is significantly higher compared to the control group. There is a positive correlation between the phagocytic number and the phagocytic index of monocytes and the value of MAFAnM. Conclusions. There was an increase of the level of monocytes in meat and egg products workers against the background of the presence of bacteria of the Staphylococcus spp. group on the skin. It may indicate the activation of pro-inflammatory factors at the level of peripheral blood. An increased percentage of staphylococcal carriers is a sign of adaptation of Staphylococcus spp. bacteria to the antibiotics used in the manufacturing process.
{"title":"INDICATORS OF THE SKIN MICROBIOTA AND THE PHAGOCYTIC ACTIVITY IN MEAT AND EGG PRODUCTION WORKERS","authors":"V. Sokolenko","doi":"10.15407/biotech15.04.041","DOIUrl":"https://doi.org/10.15407/biotech15.04.041","url":null,"abstract":"Aim. The analysis of the indicators of skin microbiota and phagocytic activity of neutrophils and monocytes in employees of the \"Peremoga Nova\" poultry farm. Methods. The presence of sanitary and epidemiologically important groups of bacteria and the number of mesophilic aerobic and facultative anaerobic microorganisms (MAFAnM) on the skin surface, leukogram parameters and phagocytic activity of professional phagocytes were determined. The indicators of students of the Bohdan Khmelnytsky National University of Cherkasy were used as a control. Results. It was found that the MAFAnM index in the experimental group (3.2×103 CFU/cm3) is significantly lower than in the control group (2.7×103 CFU/cm3), however, the percentage of Staphylococcus spp. carriers is higher (67.5% versus 40.0%). In the experimental group, the relative and total number of monocytes is significantly higher compared to the control group. There is a positive correlation between the phagocytic number and the phagocytic index of monocytes and the value of MAFAnM. Conclusions. There was an increase of the level of monocytes in meat and egg products workers against the background of the presence of bacteria of the Staphylococcus spp. group on the skin. It may indicate the activation of pro-inflammatory factors at the level of peripheral blood. An increased percentage of staphylococcal carriers is a sign of adaptation of Staphylococcus spp. bacteria to the antibiotics used in the manufacturing process.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47087740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-31DOI: 10.15407/biotech15.04.011
N. Fedosova
Aim. The purpose of the study was to evaluate the effect of B. subtilis IMV B-7724 lectin on the functional activity of macrophages (Mph) and natural killer cells (NK) of intact Balb/c mice. Materials and Methods. Balb/c mice were subjected to 10 consecutive administrations of the lectin in a dose of 1 mg/kg of body weight. The functional activity of peritoneal Mph and NK were studied. Statistical analysis of the results was performed according to the widely accepted methods of variational statistics. Results. Administration of bacterial lectin increased Mph and NK cytotoxic activity; maximal increase was registered after the complete course of injections. A significant increase in the NO production indicates the polarization of peritoneal Mph into pro-inflammatory type M1. The transcription factors of IRF (at the early stage) and STAT (at the latter stages) signalling pathways were involved in the process of Mph polarization. Conclusion. The ability of B. subtillis IMV B-7724 lectin to increase in vivo cytotoxic activity of innate immunity effectors and to maintain the long-term Mph M1 polarization urges further studies on the lectin effectiveness.
{"title":"THE EFFECT OF B. subtillis ІМV B-7724 LECTIN ON FUNCTIONAL ACTIVITY OF THE MAIN EFFECTORS OF ANTITUMOR IMMUNITY OF INTACT MICE","authors":"N. Fedosova","doi":"10.15407/biotech15.04.011","DOIUrl":"https://doi.org/10.15407/biotech15.04.011","url":null,"abstract":"Aim. The purpose of the study was to evaluate the effect of B. subtilis IMV B-7724 lectin on the functional activity of macrophages (Mph) and natural killer cells (NK) of intact Balb/c mice. Materials and Methods. Balb/c mice were subjected to 10 consecutive administrations of the lectin in a dose of 1 mg/kg of body weight. The functional activity of peritoneal Mph and NK were studied. Statistical analysis of the results was performed according to the widely accepted methods of variational statistics. Results. Administration of bacterial lectin increased Mph and NK cytotoxic activity; maximal increase was registered after the complete course of injections. A significant increase in the NO production indicates the polarization of peritoneal Mph into pro-inflammatory type M1. The transcription factors of IRF (at the early stage) and STAT (at the latter stages) signalling pathways were involved in the process of Mph polarization. Conclusion. The ability of B. subtillis IMV B-7724 lectin to increase in vivo cytotoxic activity of innate immunity effectors and to maintain the long-term Mph M1 polarization urges further studies on the lectin effectiveness.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47118704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-31DOI: 10.15407/biotech15.04.050
D. Volodkina
The use of photodynamic therapy of drugs capable of selective accumulation in the tumor or in affected cells, in particular photosensitive peptidomimetics, increases its effectiveness in various treatment schemes. Aim. Determination of weight indices (WI) of thymus and spleen in animals with carcinoma after photodynamic therapy with peptidomimetics: LMB002 and LMB033. Methods. Studies of WI of immune organs were carried out in mice of the C57 Black line on the 28th day after transplantation of Lewis lung carcinoma. The therapeutic effect was studied using photosensitive peptidomimetics: LMB002 and LMB033. The natural antibiotic gramicidin C was used as a control. Results. Comparison of the WI of the spleen of intact animals and animals with tumors showed a twofold (p< 0.05) increase in animals with tumors. As for the WI of the thymus, a tendency to its increase was observed in animals with tumors, compared to intact ones, but no significant difference was found. The following results were obtained: a decrease in the WI of the spleen in animals with tumors and the introduction of placebo and an increase in the WI of the spleen in animals treated with gramicidin C; an increase in spleen WI and a slight decrease in thymus WI under the influence of LMB002, an increase in spleen WI under the influence of LMB033, while the thymus WI did not change. Conclusions. It was established that the WI of the spleen during therapy with photosensitive peptidomimetics LMB033 and LMB002 increased in all groups of experimental animals compared to intact ones. The most significant effect on the spleen index was observed for the schemes of double administration of LMB033 and double phototherapy. No significant changes in the weight index of the thymus during therapy with photosensitive peptidomimetics LMB033 and LMB002 were found.
{"title":"THE INFLUENCE OF PHOTOSENSITIVE PEPTIDOMIMETICS ON WEIGHT INDICES OF IMMUNE ORGANS OF EXPERIMENTAL ANIMALS WITH TRANSPLANTABLE LEWIS LUNG CARCINOMA","authors":"D. Volodkina","doi":"10.15407/biotech15.04.050","DOIUrl":"https://doi.org/10.15407/biotech15.04.050","url":null,"abstract":"The use of photodynamic therapy of drugs capable of selective accumulation in the tumor or in affected cells, in particular photosensitive peptidomimetics, increases its effectiveness in various treatment schemes. Aim. Determination of weight indices (WI) of thymus and spleen in animals with carcinoma after photodynamic therapy with peptidomimetics: LMB002 and LMB033. Methods. Studies of WI of immune organs were carried out in mice of the C57 Black line on the 28th day after transplantation of Lewis lung carcinoma. The therapeutic effect was studied using photosensitive peptidomimetics: LMB002 and LMB033. The natural antibiotic gramicidin C was used as a control. Results. Comparison of the WI of the spleen of intact animals and animals with tumors showed a twofold (p< 0.05) increase in animals with tumors. As for the WI of the thymus, a tendency to its increase was observed in animals with tumors, compared to intact ones, but no significant difference was found. The following results were obtained: a decrease in the WI of the spleen in animals with tumors and the introduction of placebo and an increase in the WI of the spleen in animals treated with gramicidin C; an increase in spleen WI and a slight decrease in thymus WI under the influence of LMB002, an increase in spleen WI under the influence of LMB033, while the thymus WI did not change. Conclusions. It was established that the WI of the spleen during therapy with photosensitive peptidomimetics LMB033 and LMB002 increased in all groups of experimental animals compared to intact ones. The most significant effect on the spleen index was observed for the schemes of double administration of LMB033 and double phototherapy. No significant changes in the weight index of the thymus during therapy with photosensitive peptidomimetics LMB033 and LMB002 were found.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43815181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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