Pub Date : 2023-10-31DOI: 10.15407/biotech16.05.055
M.A Zhelavskyi
Snake venom-derived platelet aggregation inhibitors can be promising antiplatelet medications that can allow to avoid the risk of bleeding and treatment resistance, particularly in aspirin-resistant patients. Our study aimed to assess the effectiveness of a platelet aggregation inhibitor derived from Echis multisquamatis snake venom in various settings, including in vitro, in vivo, and ex vivo. Methods. We examined a polypeptide from Echis multisquamatis venom, purified using a recently developed chromatography protocol, across multiple models. This polypeptide was introduced into platelet-rich blood plasma and administered intravenously to rats. The effects on platelet aggregation were assessed using aggregometry, focusing on ADP-induced aggregation. Results & Discussion. Our findings revealed that a concentration of 0.040 mg/ml significantly reduced platelet aggregation in vitro. Remarkably, this dosage also proved effective when administered intravenously in laboratory animals, reaffirming its potential as a robust antiplatelet agent. In the final phase of our study, the polypeptide demonstrated its ability to inhibit platelet aggregation in blood plasma of pregnant woman with aspirin resistance, presenting a promising avenue for innovative treatment approaches in such cases. Conclusion. This study underscores the potential of the Echis multisquamatis venom-derived polypeptide as a promising antiplatelet agent, effective in diverse scenarios, including aspirin resistance. Further research and clinical trials are imperative to fully harness its therapeutic potential.
{"title":"APROBATION OF PLATELET AGGREGATION INHIBITOR FROM ECHIS MULTISQUAMATIS SNAKE VENOM IN VITRO, IN VIVO AND EX VIVO","authors":"M.A Zhelavskyi","doi":"10.15407/biotech16.05.055","DOIUrl":"https://doi.org/10.15407/biotech16.05.055","url":null,"abstract":"Snake venom-derived platelet aggregation inhibitors can be promising antiplatelet medications that can allow to avoid the risk of bleeding and treatment resistance, particularly in aspirin-resistant patients. Our study aimed to assess the effectiveness of a platelet aggregation inhibitor derived from Echis multisquamatis snake venom in various settings, including in vitro, in vivo, and ex vivo. Methods. We examined a polypeptide from Echis multisquamatis venom, purified using a recently developed chromatography protocol, across multiple models. This polypeptide was introduced into platelet-rich blood plasma and administered intravenously to rats. The effects on platelet aggregation were assessed using aggregometry, focusing on ADP-induced aggregation. Results & Discussion. Our findings revealed that a concentration of 0.040 mg/ml significantly reduced platelet aggregation in vitro. Remarkably, this dosage also proved effective when administered intravenously in laboratory animals, reaffirming its potential as a robust antiplatelet agent. In the final phase of our study, the polypeptide demonstrated its ability to inhibit platelet aggregation in blood plasma of pregnant woman with aspirin resistance, presenting a promising avenue for innovative treatment approaches in such cases. Conclusion. This study underscores the potential of the Echis multisquamatis venom-derived polypeptide as a promising antiplatelet agent, effective in diverse scenarios, including aspirin resistance. Further research and clinical trials are imperative to fully harness its therapeutic potential.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139307894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-31DOI: 10.15407/biotech16.05.045
M. Dudarenko
Graphene materials are widely used in different technologies and certainly released into aquatic and air surroundings being environmental pollution components. Nitrogen‑doped graphene nanomaterials have great potential for application, in particular, in energy storage, as electrochemical sensors and waste water treatment. Aim. Evaluate neurotoxic risk of nitrogen-doped multilayer graphene. Methods. Here, nitrogen-doped multilayer graphene nanoparticles (N-MLG) were synthesized by means of electrochemical exfoliation of high-purity graphite rods in NaN3-based electrolyte and characterised using TEM, AFM and UV-vis spectroscopy. Neuroactive features of N-MLG were assessed in isolated cortex nerve terminals (synaptosomes) analysing the extracellular level of excitatory neurotransmitter L-[14C] glutamate and inhibitory one [3H]GABA. Results. It was revealed that N-MLG did not affect the extracellular synaptosomal levels of L-[14C] glutamate and [3H]GABA within the concentration range 0.01–0.5 mg/ml, and an increase in a concentration up to 1 mg/ml caused an insignificant increase (tendency to increase) in these levels for both neurotransmitters. To analyse a capability of interaction with heavy metals in biological system, N-MLG was investigated using model of acute Cd2+/Pb2+/Hg2+-induced neurotoxicity in nerve terminals. In was revealed that Cd2+/Pb2+/Hg2+-induced increase in the extracellular level of L-[14C] glutamate and [3H]GABA was not changed by N-MLG. Conclusions. N-MLG does not possess neurotoxic signs and is biocompatible within the concentration range 0.01–1 mg/ml. In biological system, N-MLG did not mitigate/aggravate Cd2+/Pb2+/Hg2+-induced neurotoxicity in nerve terminals.
{"title":"ASSESSMENT OF ACUTE NEUROTOXICITY OF NITROGEN-DOPED MULTILAYER GRAPHENE NANOPARTICLES AND THEIR CAPABILITY TO CHANGE Cd2+/Pb2+/Hg2+-INDUCED INJURY IN BRAIN CORTEX NERVE TERMINALS","authors":"M. Dudarenko","doi":"10.15407/biotech16.05.045","DOIUrl":"https://doi.org/10.15407/biotech16.05.045","url":null,"abstract":"Graphene materials are widely used in different technologies and certainly released into aquatic and air surroundings being environmental pollution components. Nitrogen‑doped graphene nanomaterials have great potential for application, in particular, in energy storage, as electrochemical sensors and waste water treatment. Aim. Evaluate neurotoxic risk of nitrogen-doped multilayer graphene. Methods. Here, nitrogen-doped multilayer graphene nanoparticles (N-MLG) were synthesized by means of electrochemical exfoliation of high-purity graphite rods in NaN3-based electrolyte and characterised using TEM, AFM and UV-vis spectroscopy. Neuroactive features of N-MLG were assessed in isolated cortex nerve terminals (synaptosomes) analysing the extracellular level of excitatory neurotransmitter L-[14C] glutamate and inhibitory one [3H]GABA. Results. It was revealed that N-MLG did not affect the extracellular synaptosomal levels of L-[14C] glutamate and [3H]GABA within the concentration range 0.01–0.5 mg/ml, and an increase in a concentration up to 1 mg/ml caused an insignificant increase (tendency to increase) in these levels for both neurotransmitters. To analyse a capability of interaction with heavy metals in biological system, N-MLG was investigated using model of acute Cd2+/Pb2+/Hg2+-induced neurotoxicity in nerve terminals. In was revealed that Cd2+/Pb2+/Hg2+-induced increase in the extracellular level of L-[14C] glutamate and [3H]GABA was not changed by N-MLG. Conclusions. N-MLG does not possess neurotoxic signs and is biocompatible within the concentration range 0.01–1 mg/ml. In biological system, N-MLG did not mitigate/aggravate Cd2+/Pb2+/Hg2+-induced neurotoxicity in nerve terminals.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":"216 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139308229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-31DOI: 10.15407/biotech16.05.022
D. М. Pylypenko
The emergence of many pathogenic microorganisms, which are resistant to known antibiotics, indicates the need to find new strategies to fight them. Aim. The article is devoted to the analysis of modern research on liposomal forms of phages as a promising strategy for fighting microbial infections. Methods. Analysis of modern national and foreign research devoted to the bacteriophage encapsulation into liposomes and the evaluation of the effecacy of this drug delivery system in antimicrobial therapy. Results. Bacteriophage encapsulation into liposomal nanoparticles protects phages from the negative effects of external factors, increases the period of circulation in the organism, ensures increased bioavailability of phage particles and, as a result, increases the efficacy of antimicrobial treatment. Liposomal forms of phages have demonstrated their effectiveness in fighting many common pathogenic bacteria, including Staphylococcus aureus, Klebsiella pneumoniae, Mycobacterium tuberculosis, Salmonella, etc. Conclusions. Liposomal phages have prospects as antimicrobial drugs, however, for their widespread use in clinical practice, preclinical and clinical studies are required to confirm their effecace and safety.
{"title":"PROSPECTS FOR THE CREATION OF LIPOSOMAL ANTIMICROBIALS BASED ON PHAGES","authors":"D. М. Pylypenko","doi":"10.15407/biotech16.05.022","DOIUrl":"https://doi.org/10.15407/biotech16.05.022","url":null,"abstract":"The emergence of many pathogenic microorganisms, which are resistant to known antibiotics, indicates the need to find new strategies to fight them. Aim. The article is devoted to the analysis of modern research on liposomal forms of phages as a promising strategy for fighting microbial infections. Methods. Analysis of modern national and foreign research devoted to the bacteriophage encapsulation into liposomes and the evaluation of the effecacy of this drug delivery system in antimicrobial therapy. Results. Bacteriophage encapsulation into liposomal nanoparticles protects phages from the negative effects of external factors, increases the period of circulation in the organism, ensures increased bioavailability of phage particles and, as a result, increases the efficacy of antimicrobial treatment. Liposomal forms of phages have demonstrated their effectiveness in fighting many common pathogenic bacteria, including Staphylococcus aureus, Klebsiella pneumoniae, Mycobacterium tuberculosis, Salmonella, etc. Conclusions. Liposomal phages have prospects as antimicrobial drugs, however, for their widespread use in clinical practice, preclinical and clinical studies are required to confirm their effecace and safety.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139309429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.15407/biotech16.04.050
Sukumar Dandapat
Aim. The molecular identification of Pycnoporus sanguineus, a previously morphologically mushroom, was done to see the antibacterial activity against pathogenic bacteria Staphylococcus aureus and Salmonella typhi. Methods. A fragment of the D2 region of 28S rDNA was amplified by PCR, sequenced, and BLAST was performed using the consensus sequence. The maximum identity score was used to build a phylogenetic tree. Agar well diffusion was used to study the antibacterial activity. Results. Sequencing of a 700 base pair PCR amplicon was carried and a 616 base pair of D2 region of large subunit gene was generated. The 100 blast hits on the D2 region of LSU gene showed similarity to Trametes sanguineus voucher PRSC95 (GenBank Accession Number: JN164795.1) based on nucleotide homology and phylogenetic analysis. Antibacterial screening revealed that the crude extract had higher activity on Staphylococcus aureus, with a 3mm to 13mm zone of inhibition and a 100µg minimum inhibitory concentration, compared to Salmonella typhi. Salmonella typhi had a 5 mm to 15 mm zone of inhibition and a 200 µg minimum inhibitory concentration. Conclusion. According to the obtained result, the morphologically identified mushroom Pycnoporus sanguies can be referred to as Trametes sanguine, and it can be used for producinig antibacterial agents.
{"title":"MOLECULAR IDENTIFICATION AND ANTIBACTERIAL ACTIVITY OF MACROFUNGUS Trametes sanguineus (L.)","authors":"Sukumar Dandapat","doi":"10.15407/biotech16.04.050","DOIUrl":"https://doi.org/10.15407/biotech16.04.050","url":null,"abstract":"Aim. The molecular identification of Pycnoporus sanguineus, a previously morphologically mushroom, was done to see the antibacterial activity against pathogenic bacteria Staphylococcus aureus and Salmonella typhi. Methods. A fragment of the D2 region of 28S rDNA was amplified by PCR, sequenced, and BLAST was performed using the consensus sequence. The maximum identity score was used to build a phylogenetic tree. Agar well diffusion was used to study the antibacterial activity. Results. Sequencing of a 700 base pair PCR amplicon was carried and a 616 base pair of D2 region of large subunit gene was generated. The 100 blast hits on the D2 region of LSU gene showed similarity to Trametes sanguineus voucher PRSC95 (GenBank Accession Number: JN164795.1) based on nucleotide homology and phylogenetic analysis. Antibacterial screening revealed that the crude extract had higher activity on Staphylococcus aureus, with a 3mm to 13mm zone of inhibition and a 100µg minimum inhibitory concentration, compared to Salmonella typhi. Salmonella typhi had a 5 mm to 15 mm zone of inhibition and a 200 µg minimum inhibitory concentration. Conclusion. According to the obtained result, the morphologically identified mushroom Pycnoporus sanguies can be referred to as Trametes sanguine, and it can be used for producinig antibacterial agents.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136036217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.15407/biotech16.04.060
Yevheniia Ivchenko
Modern enzyme biotechnology is a promising and rapidly developing field that requires the latest research on the conditions of enzyme biosynthesis. Optimizing the composition of the nutrient medium depending on the needs of microorganisms and physicochemical factors directly affect the increase in the efficiency of the biosynthesis of amylolytic enzymes, namely the biosynthetic capacity of the strain Streptomyces recifensis var. lyticus 2P-15. Modulation of the biosynthetic activity of strains producing amylolytic enzymes will allow to significantly increase their economic yield. Aim. The purpose of this work is to optimize the biosynthetic capacity of the strain Streptomyces recifensis var. lyticus 2P-15 in terms of the synthesis of amylolytic enzymes and the study of the dynamics of the influence of physical and chemical factors on optimization. Methods. The object of the study is the strain Streptomyces recifensis var. lyticus 2P-15, obtained by three-stage selection of the producer. The simplex method of selecting the composition of the environment was used for the research. The ratio of amylolytic activity to the level of biomass accumulation was taken as the biosynthetic capacity of the strain. A photocolometric method was used to determine amylolytic activity. The level of biomass accumulation was determined by the weight method. Results. It was established that as a result of optimizing the composition of the simplex nutrient medium by the method of mathematical modeling, the biosynthetic capacity increased by 3.63 compared to the control variant. It was also investigated that the optimal concentration of such a component of the nutrient medium as monosodium glutamate С5Н8NO4Na・H2O is 1.5%, which increases the amylolytic activity by 2.63 and increases the accumulation of biomass. Separately, it should be noted the obtained results of the study of the optimal concentrations of heavy metal ions added to the optimized version of the nutrient medium, which allow further research in this aspect to be continued and the use of Co, Mo, Cd ions in the composition of the nutrient medium. With the obtained results, there is an increase in amylolytic activity in the best response by 3.54. The obtained results have theoretical and practical significance for further research in the biotechnology of enzymes. Conclusions. The prospect of further research into the optimization of the biosynthesis of actinomycetes by the simplex method of other aspects of its regulation will be to increase the biosynthetic capacity of the studied strain, which will have a positive effect on the economic output of the production of amylolytic enzyme preparations by obtaining microbial synthesis.
现代酶生物技术是一个前景广阔、发展迅速的领域，需要对酶的生物合成条件进行最新的研究。根据微生物的需要和理化因素对营养培养基的组成进行优化，直接影响到解淀粉酶生物合成效率的提高，即解淀粉链霉菌(Streptomyces refensis var. lyticus 2P-15)的生物合成能力。调节生产淀粉酶的菌株的生物合成活性将使其经济产量显著提高。的目标。本研究旨在从解淀粉酶的合成方面对重组链霉菌裂解变种2P-15的生物合成能力进行优化，并研究理化因素对优化的动态影响。方法。本研究以经三期筛选获得的累解链霉菌裂解变种2P-15菌株为研究对象。采用单纯形法选择环境成分进行研究。以淀粉水解活性与生物量积累水平之比作为菌株的生物合成能力。采用光比色法测定淀粉水解活性。生物量积累水平采用重量法测定。结果。结果表明，通过数学建模的方法优化单形培养基的组成，单形培养基的生物合成能力比对照菌株提高了3.63%。研究还发现，在营养培养基中添加味精С5Н8NO4Na·H2O等成分的最佳浓度为1.5%时，可使淀粉水解活性提高2.63，增加生物量的积累。另外，值得注意的是，所获得的优化版营养培养基中重金属离子的最佳添加浓度的研究结果，可以继续进行这方面的进一步研究，并在营养培养基的组成中使用Co、Mo、Cd离子。根据所得结果，最佳反应的解淀粉活性提高了3.54。所得结果对酶生物技术的进一步研究具有理论和实际意义。结论。进一步研究利用单纯形法优化放线菌生物合成的其他方面调控的前景将是提高所研究菌株的生物合成能力，这将对获得微生物合成的淀粉酶制剂生产的经济产量产生积极影响。
{"title":"FACTORS ON THE BIOSYNTHESIS OF AMYLOLITIC ENZYMES OF STREPTOMYCETE ORIGIN","authors":"Yevheniia Ivchenko","doi":"10.15407/biotech16.04.060","DOIUrl":"https://doi.org/10.15407/biotech16.04.060","url":null,"abstract":"Modern enzyme biotechnology is a promising and rapidly developing field that requires the latest research on the conditions of enzyme biosynthesis. Optimizing the composition of the nutrient medium depending on the needs of microorganisms and physicochemical factors directly affect the increase in the efficiency of the biosynthesis of amylolytic enzymes, namely the biosynthetic capacity of the strain Streptomyces recifensis var. lyticus 2P-15. Modulation of the biosynthetic activity of strains producing amylolytic enzymes will allow to significantly increase their economic yield. Aim. The purpose of this work is to optimize the biosynthetic capacity of the strain Streptomyces recifensis var. lyticus 2P-15 in terms of the synthesis of amylolytic enzymes and the study of the dynamics of the influence of physical and chemical factors on optimization. Methods. The object of the study is the strain Streptomyces recifensis var. lyticus 2P-15, obtained by three-stage selection of the producer. The simplex method of selecting the composition of the environment was used for the research. The ratio of amylolytic activity to the level of biomass accumulation was taken as the biosynthetic capacity of the strain. A photocolometric method was used to determine amylolytic activity. The level of biomass accumulation was determined by the weight method. Results. It was established that as a result of optimizing the composition of the simplex nutrient medium by the method of mathematical modeling, the biosynthetic capacity increased by 3.63 compared to the control variant. It was also investigated that the optimal concentration of such a component of the nutrient medium as monosodium glutamate С5Н8NO4Na・H2O is 1.5%, which increases the amylolytic activity by 2.63 and increases the accumulation of biomass. Separately, it should be noted the obtained results of the study of the optimal concentrations of heavy metal ions added to the optimized version of the nutrient medium, which allow further research in this aspect to be continued and the use of Co, Mo, Cd ions in the composition of the nutrient medium. With the obtained results, there is an increase in amylolytic activity in the best response by 3.54. The obtained results have theoretical and practical significance for further research in the biotechnology of enzymes. Conclusions. The prospect of further research into the optimization of the biosynthesis of actinomycetes by the simplex method of other aspects of its regulation will be to increase the biosynthetic capacity of the studied strain, which will have a positive effect on the economic output of the production of amylolytic enzyme preparations by obtaining microbial synthesis.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136036559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.15407/biotech16.04.031
I.Y. Shchenyavskyi
Cardiovascular diseases are currently the most common cause of death worldwide. In this regard, experimental and clinical studies of the effectiveness of therapy of ischemic and non-ischemic heart diseases using stem cells are relevant. The purpose of this review was to evaluate the prospects of using cord blood stem cells in the treatment of cardiovascular diseases. Methods. The following databases were searched: «BIGG International database of GRADE guidelines», “Database of GRADE EtD's and Guidelines”, “Dynamed”, “ebmafrica.net”, “ECRI”, “MAGIC authoring and publication platform (MAGICapp)”, “National Health and Medical Research Council (NHMRC) portal”, “NICE Evidence”, “Pubmed”, “TRIP database”, “U.S. Preventive Services Task Force”. Results. An analysis of research related to this problem, which was conducted in recent years, was made, and considerations regarding the prospects of using umbilical cord blood in the treatment of diseases of the cardiovascular system were outlined. Conclusions. Despite some successes, realizing the full potential of cord blood stem cells in the treatment of cardiovascular diseases still requires further serious, targeted and well-funded research and expanded clinical trials.
{"title":"PROSPECTS FOR THE USE OF UMBILICAL CORD BLOOD IN THE TREATMENT OF DISEASES OF THE CARDIOVASCULAR SYSTEM","authors":"I.Y. Shchenyavskyi","doi":"10.15407/biotech16.04.031","DOIUrl":"https://doi.org/10.15407/biotech16.04.031","url":null,"abstract":"Cardiovascular diseases are currently the most common cause of death worldwide. In this regard, experimental and clinical studies of the effectiveness of therapy of ischemic and non-ischemic heart diseases using stem cells are relevant. The purpose of this review was to evaluate the prospects of using cord blood stem cells in the treatment of cardiovascular diseases. Methods. The following databases were searched: «BIGG International database of GRADE guidelines», “Database of GRADE EtD's and Guidelines”, “Dynamed”, “ebmafrica.net”, “ECRI”, “MAGIC authoring and publication platform (MAGICapp)”, “National Health and Medical Research Council (NHMRC) portal”, “NICE Evidence”, “Pubmed”, “TRIP database”, “U.S. Preventive Services Task Force”. Results. An analysis of research related to this problem, which was conducted in recent years, was made, and considerations regarding the prospects of using umbilical cord blood in the treatment of diseases of the cardiovascular system were outlined. Conclusions. Despite some successes, realizing the full potential of cord blood stem cells in the treatment of cardiovascular diseases still requires further serious, targeted and well-funded research and expanded clinical trials.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136037234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.15407/biotech16.04.044
І.О. Nitovska
The aim of the work was to investigate detection of different modifications of the green fluorescent protein gene (gfp) in the transgenic tobacco and maize plants by polymerase chain reaction (PCR). Methods. Total DNA isolation, PCR, electrophoresis of DNA in agarose gel, bioinformatic resources. Results. Three pairs of primers were used for PCR analysis of tobacco and maize containing wild-type gfp or mutant synthetic gene S65Tpgfp. The primer pair gfp1F-gfp1R interacted with the wild-type gfp gene only. The gfp2F-gfp2R primers interacted with the gfp gene of different modifications both in tobacco and maize. The gfp3F-gfp3R primer pair interacted with the modified S65Tpgfp gene in tobacco DNA, but not with maize samples. Conclusions. Primers for detection of heterologous gfp gene, which were both narrowly specific (only one gene modification could be detected), and universal (more than one gene modification could be detected), were verified. It was shown that the primer pair gfp2F-gfp2R was universal for gfp gene detection both in tobacco and maize plants by PCR. The results obtained with gfp2F-gfp2R were reliably reproducible, so this primer pair is recommended for general use.
{"title":"PECULIARITIES OF GREEN FLUORESCENT PROTEIN TRANSGENE DETECTION IN TOBACCO AND MAIZE PLANTS BY PCR","authors":"І.О. Nitovska","doi":"10.15407/biotech16.04.044","DOIUrl":"https://doi.org/10.15407/biotech16.04.044","url":null,"abstract":"The aim of the work was to investigate detection of different modifications of the green fluorescent protein gene (gfp) in the transgenic tobacco and maize plants by polymerase chain reaction (PCR). Methods. Total DNA isolation, PCR, electrophoresis of DNA in agarose gel, bioinformatic resources. Results. Three pairs of primers were used for PCR analysis of tobacco and maize containing wild-type gfp or mutant synthetic gene S65Tpgfp. The primer pair gfp1F-gfp1R interacted with the wild-type gfp gene only. The gfp2F-gfp2R primers interacted with the gfp gene of different modifications both in tobacco and maize. The gfp3F-gfp3R primer pair interacted with the modified S65Tpgfp gene in tobacco DNA, but not with maize samples. Conclusions. Primers for detection of heterologous gfp gene, which were both narrowly specific (only one gene modification could be detected), and universal (more than one gene modification could be detected), were verified. It was shown that the primer pair gfp2F-gfp2R was universal for gfp gene detection both in tobacco and maize plants by PCR. The results obtained with gfp2F-gfp2R were reliably reproducible, so this primer pair is recommended for general use.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136036754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.15407/biotech16.04.005
N. V. Borzova
One of the important problems of current biotechnology is the usage of enzymes of microbial origin for destruction of poorly soluble compounds and synthesis of new drugs. In recent years a great deal of researchers’ attention has been given to such technologically promising carbohydrases as O-glycosylhydrolases catalyzing the hydrolysis of O-glycoside links in glycosides, oligo- and polysaccharides, glycolipids, and other glycoconjugates. Aim. The review provides data on the position of α-L-rhamnosidases in the modern hierarchical classification of glycosidases and presents data available in the literature on the features of the enzyme structure in various microorganisms. Methods. The publications from the following databases were analyzed: PubMed (https://pubmed.nsbi.nlm.nih.gov/), the Carbohydrate-Active enZYmes (http://www.cazy.org/), the BRENDA Enzyme Database (https://www.brenda-enzymes.org/). Results. Data on the physicochemical, catalytic, and kinetic properties of α-L-rhamnosidases in microorganisms of different taxonomic groups have been systematized. The peculiarities of the substrate specificity of the enzyme depending on the nature of the protein and the growing conditions of the producer are characterized. Conclusions. Functional properties and specificity action of microbial α-L-rhamnosidases suggest their broad-range applicability for food and animal feed processing, as well as obtaining biologically active compounds for the pharmaceutical industry and medicine.
当前生物技术的一个重要问题是利用微生物来源的酶来破坏难溶性化合物和合成新药。近年来，研究人员将大量的注意力放在了一些技术上很有前途的糖酶上，如o-糖基水解酶，这些酶可以催化糖苷、低聚糖和多糖、糖脂和其他糖缀合物中的o-糖苷键的水解。的目标。本文综述了α- l -鼠李糖苷酶在现代糖苷酶等级分类中的地位，并介绍了各种微生物中该酶结构特征的文献资料。方法。分析了来自以下数据库的出版物:PubMed (https://pubmed.nsbi.nlm.nih.gov/)，碳水化合物活性酶(http://www.cazy.org/)， BRENDA酶数据库(https://www.brenda-enzymes.org/)。结果。对不同分类类群微生物中α- l -鼠李糖苷酶的理化、催化和动力学性质进行了系统的研究。酶的底物特异性的特点取决于蛋白质的性质和生产者的生长条件。结论。微生物α- l -鼠李糖苷酶的功能特性和特异性作用表明其在食品和动物饲料加工以及制药工业和医药中获得生物活性化合物方面具有广泛的适用性。
{"title":"MICROBIAL α-L-RHAMNOSIDASES: CLASSIFICATION, DISTRIBUTION, PROPERTIES AND PRACTICAL APPLICATION","authors":"N. V. Borzova","doi":"10.15407/biotech16.04.005","DOIUrl":"https://doi.org/10.15407/biotech16.04.005","url":null,"abstract":"One of the important problems of current biotechnology is the usage of enzymes of microbial origin for destruction of poorly soluble compounds and synthesis of new drugs. In recent years a great deal of researchers’ attention has been given to such technologically promising carbohydrases as O-glycosylhydrolases catalyzing the hydrolysis of O-glycoside links in glycosides, oligo- and polysaccharides, glycolipids, and other glycoconjugates. Aim. The review provides data on the position of α-L-rhamnosidases in the modern hierarchical classification of glycosidases and presents data available in the literature on the features of the enzyme structure in various microorganisms. Methods. The publications from the following databases were analyzed: PubMed (https://pubmed.nsbi.nlm.nih.gov/), the Carbohydrate-Active enZYmes (http://www.cazy.org/), the BRENDA Enzyme Database (https://www.brenda-enzymes.org/). Results. Data on the physicochemical, catalytic, and kinetic properties of α-L-rhamnosidases in microorganisms of different taxonomic groups have been systematized. The peculiarities of the substrate specificity of the enzyme depending on the nature of the protein and the growing conditions of the producer are characterized. Conclusions. Functional properties and specificity action of microbial α-L-rhamnosidases suggest their broad-range applicability for food and animal feed processing, as well as obtaining biologically active compounds for the pharmaceutical industry and medicine.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":"2015 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136036219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.15407/biotech16.04.022
L.P. Buchatskyi
Vesiculoviruses are widely used in various fields of biotechnology. This article analyzes the results of published experimental works devoted to the development of oncolytic and recombinant vaccines against emergent viral infections based on vesiculoviruses. The use of genetic engineering methods makes it possible to strengthen their immunogenicity and oncolytic potential. Aim. Analysis and summarization of available information devoted to the development of oncolytic and other vaccines based on vesiculoviruses. Materials and methods. Publications were selected based on the PubMed (https://pubmed.ncbi.nlm.nih.gov/) and Google Scholar (https://scholar.google.com/) databases published in 2010–2023. They include information on development of oncolytic and other vaccines based on vesiculoviruses. Resalts. The article describes in detail the use of vesiculoviruses as a tool for creating highly active recombinant viral vaccines. These vaccines are able to protect people from emergent viral infections in various countries and may find application in anticancer therapy.
{"title":"VESICULOVIRUSES AS A TOOL OF BIOTECHNOLOGY","authors":"L.P. Buchatskyi","doi":"10.15407/biotech16.04.022","DOIUrl":"https://doi.org/10.15407/biotech16.04.022","url":null,"abstract":"Vesiculoviruses are widely used in various fields of biotechnology. This article analyzes the results of published experimental works devoted to the development of oncolytic and recombinant vaccines against emergent viral infections based on vesiculoviruses. The use of genetic engineering methods makes it possible to strengthen their immunogenicity and oncolytic potential. Aim. Analysis and summarization of available information devoted to the development of oncolytic and other vaccines based on vesiculoviruses. Materials and methods. Publications were selected based on the PubMed (https://pubmed.ncbi.nlm.nih.gov/) and Google Scholar (https://scholar.google.com/) databases published in 2010–2023. They include information on development of oncolytic and other vaccines based on vesiculoviruses. Resalts. The article describes in detail the use of vesiculoviruses as a tool for creating highly active recombinant viral vaccines. These vaccines are able to protect people from emergent viral infections in various countries and may find application in anticancer therapy.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136036755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.15407/biotech16.03.005
Yuliya Skril
An analytical review of the biotechnological process of production of various hard and semi-hard cheeses in the EU and Ukraine, as well as domestic recipes of fermented cheeses for production at craft cheese factories and at home, was conducted. An analysis of the conditions of the key stages of production, including fermentation, coagulation and ripening, was carried out. The composition and type of lactic acid bacteria in sourdough for fermented cheeses, as well as enzymes for fermentolysis and coagulation of milk casein, were studied. As a result of a complex study, a total of 73 types of hard and semi-hard cheeses were analyzed: 35 recipes of the New England Cheesemaking Supply Company by Jim Wallace; 30 production processes of hard and semi-hard cheeses from the EU; 8 technical conditions of hard cheeses of Ukrainian producers. It is shown that the prospects for optimizing the development of new types of hard and semi-hard cheeses in Ukraine are mainly related to the regulation of the time and temperature of fermentation, cooking and ripening of cheeses, as well as the expansion of the biodiversity of the primary and secondary microbiome of starter cultures to improve the taste and aroma of the ready-to-use product. Purpose: to analyze the fermentation process and recipes for the production of hard cheeses in Ukraine with the main world samples, to compare the composition and type of lactic acid bacteria in industrial and craft starters, as well as the types of enzymes for fermentolysis and casein coagulation of milk, in order to optimize production to improve the taste and aroma of ready-to-use product. Materials and methods. Methodical analysis and abstract-logical method for summarizing the evaluation criteria of the biotechnological process of various hard and semi-hard domestic cheeses with world samples according to recommendations, requirements and standards with the development of patents, technical conditions of their production in the EU and Ukraine; DSTU 6003 (Solid cheeses); New England Cheesemaking Supply Company cheese recipes by Jim Wallace. The obtained data were processed by methods of statistical analysis, systematization, comparison and generalization of information. Results. In this study, the documentation was examined and the data of standards, regulations, requirements and recommendations regarding the biotechnology of hard and semi-hard cheeses were analyzed, an analysis of the market of hard cheeses and the peculiarities of the fermentation process of their production was carried out with the determination of critical points and key stages of production using industrial deposited fermentation producers and protein enzymes coagulation and biodiversity of lactic acid bacteria in sourdoughs for fermented cheeses at world productions, with an assessment of the prospects for developing new and improving the biotechnology of Ukrainian benign and safe hard cheeses for healthy nutrition.
对欧盟和乌克兰生产各种硬奶酪和半硬奶酪的生物技术过程以及在手工奶酪工厂和国内生产的发酵奶酪配方进行了分析审查。对发酵、混凝和成熟等关键生产阶段的条件进行了分析。研究了奶酪发酵酵母中乳酸菌的组成和类型，以及牛奶酪蛋白的发酵和凝固酶。经过一项复杂的研究，总共分析了73种硬奶酪和半硬奶酪:吉姆·华莱士(Jim Wallace)的新英格兰奶酪制造供应公司(New England cheesmaker Supply Company)的35种食谱;来自欧盟的30种硬奶酪和半硬奶酪的生产工艺;乌克兰硬奶酪生产商的8项技术条件。结果表明，在乌克兰，优化新型硬奶酪和半硬奶酪开发的前景主要与调节奶酪发酵、烹饪和成熟的时间和温度，以及扩大发酵剂初级和次级微生物组的生物多样性有关，以改善即用产品的口感和香气。目的:与世界主要样品分析乌克兰硬奶酪的发酵工艺和配方，比较工业发酵剂和工艺发酵剂中乳酸菌的组成和类型，以及牛奶的发酵酶和酪蛋白凝固酶的类型，以优化生产，改善即用产品的口感和香气。材料和方法。方法分析和抽象逻辑的方法总结了各种硬质和半硬质奶酪的生物技术过程的评价标准与世界样品，根据专利的发展要求和标准，在欧盟和乌克兰的生产技术条件;DSTU 6003(固体奶酪);新英格兰奶酪制作供应公司的奶酪食谱由吉姆华莱士。通过统计分析、系统化、比较和信息概括等方法对所得数据进行处理。结果。在本研究中，研究了文献资料，并分析了有关硬奶酪和半硬奶酪生物技术的标准、法规、要求和建议的数据。分析了硬奶酪的市场和其生产的发酵过程的特点，确定了生产的关键点和关键阶段，使用工业沉积发酵生产者和蛋白酶，凝固和乳酸菌的生物多样性在发酵奶酪的酵母在世界生产。评估了开发新的和改进乌克兰良性和安全硬奶酪的生物技术以促进健康营养的前景。
{"title":"ANALYTICAL REVIEW OF BIOTECHNOLOGICAL PROBLEM OF UKRAINIAN HARD CHEESES","authors":"Yuliya Skril","doi":"10.15407/biotech16.03.005","DOIUrl":"https://doi.org/10.15407/biotech16.03.005","url":null,"abstract":"An analytical review of the biotechnological process of production of various hard and semi-hard cheeses in the EU and Ukraine, as well as domestic recipes of fermented cheeses for production at craft cheese factories and at home, was conducted. An analysis of the conditions of the key stages of production, including fermentation, coagulation and ripening, was carried out. The composition and type of lactic acid bacteria in sourdough for fermented cheeses, as well as enzymes for fermentolysis and coagulation of milk casein, were studied. As a result of a complex study, a total of 73 types of hard and semi-hard cheeses were analyzed: 35 recipes of the New England Cheesemaking Supply Company by Jim Wallace; 30 production processes of hard and semi-hard cheeses from the EU; 8 technical conditions of hard cheeses of Ukrainian producers. It is shown that the prospects for optimizing the development of new types of hard and semi-hard cheeses in Ukraine are mainly related to the regulation of the time and temperature of fermentation, cooking and ripening of cheeses, as well as the expansion of the biodiversity of the primary and secondary microbiome of starter cultures to improve the taste and aroma of the ready-to-use product. Purpose: to analyze the fermentation process and recipes for the production of hard cheeses in Ukraine with the main world samples, to compare the composition and type of lactic acid bacteria in industrial and craft starters, as well as the types of enzymes for fermentolysis and casein coagulation of milk, in order to optimize production to improve the taste and aroma of ready-to-use product. Materials and methods. Methodical analysis and abstract-logical method for summarizing the evaluation criteria of the biotechnological process of various hard and semi-hard domestic cheeses with world samples according to recommendations, requirements and standards with the development of patents, technical conditions of their production in the EU and Ukraine; DSTU 6003 (Solid cheeses); New England Cheesemaking Supply Company cheese recipes by Jim Wallace. The obtained data were processed by methods of statistical analysis, systematization, comparison and generalization of information. Results. In this study, the documentation was examined and the data of standards, regulations, requirements and recommendations regarding the biotechnology of hard and semi-hard cheeses were analyzed, an analysis of the market of hard cheeses and the peculiarities of the fermentation process of their production was carried out with the determination of critical points and key stages of production using industrial deposited fermentation producers and protein enzymes coagulation and biodiversity of lactic acid bacteria in sourdoughs for fermented cheeses at world productions, with an assessment of the prospects for developing new and improving the biotechnology of Ukrainian benign and safe hard cheeses for healthy nutrition.","PeriodicalId":9267,"journal":{"name":"Biotechnologia Acta","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41405559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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