Pub Date : 2018-12-30DOI: 10.33687/ZOOBIOL.001.01.1579
Helena Schneider, D. Fischer, K. Failing, C. Ehling, S. Meinecke-Tillmann, A. Wehrend, M. Lierz
The aim of the present study was the modification and evaluation of three different semen extenders for cockatiel semen in order to achieve a long survival time for transport, examination purpose and for potential cryopreservation, respectively. Therefore, individual and pooled semen samples of 30 cockatiels (Nymphicus hollandicus) were investigated for pH and osmolality values and subsequently pH and osmolality values of the semen extenders were adjusted to those values in the semen. Pooled semen samples were then partitioned into four equal parts and diluted with the three different semen extenders in 1:4 and 1:8 dilution. 1 % glucose-Ringer’s solution was used as control, respectively. A total of 64 incremental diluted semen samples were obtained for investigation. Each dilution was investigated regarding sperm motility immediately after dilution and another four times every 30 minutes. Sperm viability was evaluated 0 and 120 minutes after dilution via eosin B-stain on the diluted semen samples and in pure semen samples. Additionally, the fluorescence stain SYBR® Green/propidium iodide was used to assess sperm viability. The results indicate that cockatiel spermatozoa are highly sensitive to variations in pH and osmolality, requiring adjustment of commercial diluents to pH = 7.42 and osmolality = 300 mOsm/kg. Modified Lake diluent maintained higher viability and motility than other diluents tested. Sperm morphology was indicated to be least adversely affected by modified Lake diluent in 1:4 concentration compared to other semen extenders and concentrations used.
{"title":"Investigations on different Semen Extenders for Cockatiel Semen","authors":"Helena Schneider, D. Fischer, K. Failing, C. Ehling, S. Meinecke-Tillmann, A. Wehrend, M. Lierz","doi":"10.33687/ZOOBIOL.001.01.1579","DOIUrl":"https://doi.org/10.33687/ZOOBIOL.001.01.1579","url":null,"abstract":"The aim of the present study was the modification and evaluation of three different semen extenders for cockatiel semen in order to achieve a long survival time for transport, examination purpose and for potential cryopreservation, respectively. Therefore, individual and pooled semen samples of 30 cockatiels (Nymphicus hollandicus) were investigated for pH and osmolality values and subsequently pH and osmolality values of the semen extenders were adjusted to those values in the semen. Pooled semen samples were then partitioned into four equal parts and diluted with the three different semen extenders in 1:4 and 1:8 dilution. 1 % glucose-Ringer’s solution was used as control, respectively. A total of 64 incremental diluted semen samples were obtained for investigation. Each dilution was investigated regarding sperm motility immediately after dilution and another four times every 30 minutes. Sperm viability was evaluated 0 and 120 minutes after dilution via eosin B-stain on the diluted semen samples and in pure semen samples. Additionally, the fluorescence stain SYBR® Green/propidium iodide was used to assess sperm viability. The results indicate that cockatiel spermatozoa are highly sensitive to variations in pH and osmolality, requiring adjustment of commercial diluents to pH = 7.42 and osmolality = 300 mOsm/kg. Modified Lake diluent maintained higher viability and motility than other diluents tested. Sperm morphology was indicated to be least adversely affected by modified Lake diluent in 1:4 concentration compared to other semen extenders and concentrations used.","PeriodicalId":92816,"journal":{"name":"Journal of zoo biology","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79623630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-30DOI: 10.33687/ZOOBIOL.001.01.2004
Yukari Sotohira, H. Okui, Kazuyuki Suzuki, M. Asakawa, T. Sano
Kangaroo disease (lumpy jaw disease; LJD) is a disease of the oral cavity in Macropodidae that may be caused by stress-related factors; however, detailed information about its pathogenesis is lacking. Therefore, in this study, we evaluated markers of stress in kangaroos with and without LJD to determine the factors that cause an LJD outbreak. We evaluated the oxidative stress value, antioxidant activity, and plasma cortisol concentration in blood samples. Additionally, we measured the cortisol concentration in saliva samples. The oxidative stress value and serum cortisol concentrations were statistically significantly different between the two groups, but the antioxidant activity and saliva cortisol concentrations did not differ significantly. Relatively large variations were observed for each value within individuals.
{"title":"Association Between the Levels of Stress Markers and the Onset of Kangaroo Disease (Lumpy Jaw Disease) in Captive Kangaroos","authors":"Yukari Sotohira, H. Okui, Kazuyuki Suzuki, M. Asakawa, T. Sano","doi":"10.33687/ZOOBIOL.001.01.2004","DOIUrl":"https://doi.org/10.33687/ZOOBIOL.001.01.2004","url":null,"abstract":"Kangaroo disease (lumpy jaw disease; LJD) is a disease of the oral cavity in Macropodidae that may be caused by stress-related factors; however, detailed information about its pathogenesis is lacking. Therefore, in this study, we evaluated markers of stress in kangaroos with and without LJD to determine the factors that cause an LJD outbreak. We evaluated the oxidative stress value, antioxidant activity, and plasma cortisol concentration in blood samples. Additionally, we measured the cortisol concentration in saliva samples. The oxidative stress value and serum cortisol concentrations were statistically significantly different between the two groups, but the antioxidant activity and saliva cortisol concentrations did not differ significantly. Relatively large variations were observed for each value within individuals.","PeriodicalId":92816,"journal":{"name":"Journal of zoo biology","volume":"109 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73133726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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