Pub Date : 2019-01-17DOI: 10.19080/IJCSMB.2019.05.555668
W. Onuigbo
A rare form of laryngeal carcinoma is the undifferentiated form. There are but a few cases in the literature. Therefore, two cases from among the Ibos of South Eastern Nigeria are deemed to be worthy of documentation.
{"title":"Undifferentiated Carcinoma of the Larynx in a Developing Community","authors":"W. Onuigbo","doi":"10.19080/IJCSMB.2019.05.555668","DOIUrl":"https://doi.org/10.19080/IJCSMB.2019.05.555668","url":null,"abstract":"A rare form of laryngeal carcinoma is the undifferentiated form. There are but a few cases in the literature. Therefore, two cases from among the Ibos of South Eastern Nigeria are deemed to be worthy of documentation.","PeriodicalId":93190,"journal":{"name":"International journal of cell science & molecular biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44868217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-14DOI: 10.19080/IJCSMB.2019.05.555667
Obroucheva Nv
Abscisic Acid (ABA) is the phytohormone inducing seed dormancy, whereas seed germination occurs at low ABA concentration. Two pathways of their regulation are considered. One represents ABA reception and its signal transduction to ABA-responsive genes to induce dormancy genes, a modification of this way leading to germination in the absence of ABA. Another way suggests direct ABA inhibition of plasmamembrane H+-ATPase in dormant seeds that prevents germination and its activation in the absence of ABA, thus promoting seed germination.
{"title":"Distinct Pathways of Regulation Seed Dormancy and Germination by Abscisic Acid","authors":"Obroucheva Nv","doi":"10.19080/IJCSMB.2019.05.555667","DOIUrl":"https://doi.org/10.19080/IJCSMB.2019.05.555667","url":null,"abstract":"Abscisic Acid (ABA) is the phytohormone inducing seed dormancy, whereas seed germination occurs at low ABA concentration. Two pathways of their regulation are considered. One represents ABA reception and its signal transduction to ABA-responsive genes to induce dormancy genes, a modification of this way leading to germination in the absence of ABA. Another way suggests direct ABA inhibition of plasmamembrane H+-ATPase in dormant seeds that prevents germination and its activation in the absence of ABA, thus promoting seed germination.","PeriodicalId":93190,"journal":{"name":"International journal of cell science & molecular biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49452520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-11DOI: 10.19080/IJCSMB.2019.05.555666
S. Sreehari
Primary malignant non-Hodgkin’s lymphomas in the female genital tract are rare, accounting for less than 1% of all extranodal non-Hodgkin’s lymphomas. HPV infection has well been documented as the causative factor for Carcinoma cervix; not malignant lymphoma of cervix. Here we report an interesting case of primary non-Hodgkin’s lymphomas of uterine cervix with proven HPV 16 infection and High grade squamous intraepithelial lesion with co-existing diffuse large B-cell lymphomas (DLBCL) on histopathological examination and immunohistochemical study. The patient was successfully treated with chemotherapy after Cervical Conisation for HSIL and is now in complete remission with follow up period of 11 months. Gynecological lymphomas can be a diagnostic challenge due to nonspecific symptoms. However, when diagnosed and treated they have a good prognosis. Clinicians should therefore include lymphoma in their differential diagnosis when investigating gynecological symptoms.
{"title":"High Risk HPV, HSIL and Primary Diffuse Large B Cell Lymphoma of Cervix: An Unsual Case","authors":"S. Sreehari","doi":"10.19080/IJCSMB.2019.05.555666","DOIUrl":"https://doi.org/10.19080/IJCSMB.2019.05.555666","url":null,"abstract":"Primary malignant non-Hodgkin’s lymphomas in the female genital tract are rare, accounting for less than 1% of all extranodal non-Hodgkin’s lymphomas. HPV infection has well been documented as the causative factor for Carcinoma cervix; not malignant lymphoma of cervix. Here we report an interesting case of primary non-Hodgkin’s lymphomas of uterine cervix with proven HPV 16 infection and High grade squamous intraepithelial lesion with co-existing diffuse large B-cell lymphomas (DLBCL) on histopathological examination and immunohistochemical study. The patient was successfully treated with chemotherapy after Cervical Conisation for HSIL and is now in complete remission with follow up period of 11 months. Gynecological lymphomas can be a diagnostic challenge due to nonspecific symptoms. However, when diagnosed and treated they have a good prognosis. Clinicians should therefore include lymphoma in their differential diagnosis when investigating gynecological symptoms.","PeriodicalId":93190,"journal":{"name":"International journal of cell science & molecular biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42737576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-09DOI: 10.19080/IJCSMB.2019.05.555665
W. Onuigbo
Squamous cell carcinoma of the tongue is characterized by malignant cells which show squamous differentiation in the direction of “formation of keratin and/or the presence of intercellular bridges.” As single case reports have appeared in the literature recently, this paper presents a good number of them traced among the Ibo ethnic group in Nigeria, this being facilitated by the author who heads an institution that provides a histopathology data pool.
{"title":"Squamous Cell Carcinoma of The Tongue in A Developing Community","authors":"W. Onuigbo","doi":"10.19080/IJCSMB.2019.05.555665","DOIUrl":"https://doi.org/10.19080/IJCSMB.2019.05.555665","url":null,"abstract":"Squamous cell carcinoma of the tongue is characterized by malignant cells which show squamous differentiation in the direction of “formation of keratin and/or the presence of intercellular bridges.” As single case reports have appeared in the literature recently, this paper presents a good number of them traced among the Ibo ethnic group in Nigeria, this being facilitated by the author who heads an institution that provides a histopathology data pool.","PeriodicalId":93190,"journal":{"name":"International journal of cell science & molecular biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43030169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-08DOI: 10.19080/IJCSMB.2019.05.555664
W. Onuigbo
{"title":"Basosquamous Carcinoma of Skin in A Developing Community","authors":"W. Onuigbo","doi":"10.19080/IJCSMB.2019.05.555664","DOIUrl":"https://doi.org/10.19080/IJCSMB.2019.05.555664","url":null,"abstract":"","PeriodicalId":93190,"journal":{"name":"International journal of cell science & molecular biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42658934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ana M Blázquez Medela, Ashley Penton, Kristina I Bostrom, Arman Saparov, Medet Jumabay
Multipotent cells derived from white mature adipocytes, referred to as dedifferentiated fat (DFAT) cells have the capacity differentiate into endothelial cells. The objective of this study was to modify the isolation method for DFAT cells in order to optimize the endothelial lineage potential. The adipocytes were preincubated for 24 hours, washed, and then incubated for 5 days to allow the generated DFAT cells to remain in proximity to the adipocytes while the cells aggregated into cell clusters. The DFAT cells rapidly differentiated into adipocytes after which endothelial-like cells (ECs) emerged and formed tube-like structure closely associated with the newly differentiated adipocytes. The lipid-filled cells then gradually disappeared whereas the network of tube structure expanded over the course of 3 weeks. ECs accounted for 35-45% of the cells derived from the DFAT cells, as assessed by qPCR, immunofluorescence and fluorescence-activated cell sorting. The DFAT cell-derived ECs could also be further enriched by magnetic sorting, thereby serving as a mouse cell line for further research.
来自白色成熟脂肪细胞的多能细胞，称为去分化脂肪(DFAT)细胞，具有分化为内皮细胞的能力。本研究的目的是改进DFAT细胞的分离方法，以优化内皮细胞系的潜力。脂肪细胞预孵育24小时，清洗，然后孵育5天，使生成的DFAT细胞保持在脂肪细胞附近，同时细胞聚集成细胞簇。DFAT细胞迅速分化为脂肪细胞，内皮样细胞(endothelial like cells, ECs)出现并形成管状结构，与新分化的脂肪细胞密切相关。3周后，脂质细胞逐渐消失，而管状结构网络逐渐扩大。通过qPCR、免疫荧光和荧光激活细胞分选评估，ECs占DFAT细胞衍生细胞的35-45%。DFAT细胞衍生的ECs也可以通过磁分选进一步富集，从而作为小鼠细胞系进行进一步研究。
{"title":"Generation of Vascular Networks from Adipocytes <i>In Vitro</i>.","authors":"Ana M Blázquez Medela, Ashley Penton, Kristina I Bostrom, Arman Saparov, Medet Jumabay","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Multipotent cells derived from white mature adipocytes, referred to as dedifferentiated fat (DFAT) cells have the capacity differentiate into endothelial cells. The objective of this study was to modify the isolation method for DFAT cells in order to optimize the endothelial lineage potential. The adipocytes were preincubated for 24 hours, washed, and then incubated for 5 days to allow the generated DFAT cells to remain in proximity to the adipocytes while the cells aggregated into cell clusters. The DFAT cells rapidly differentiated into adipocytes after which endothelial-like cells (ECs) emerged and formed tube-like structure closely associated with the newly differentiated adipocytes. The lipid-filled cells then gradually disappeared whereas the network of tube structure expanded over the course of 3 weeks. ECs accounted for 35-45% of the cells derived from the DFAT cells, as assessed by qPCR, immunofluorescence and fluorescence-activated cell sorting. The DFAT cell-derived ECs could also be further enriched by magnetic sorting, thereby serving as a mouse cell line for further research.</p>","PeriodicalId":93190,"journal":{"name":"International journal of cell science & molecular biology","volume":"6 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8095932/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38954379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-04DOI: 10.19080/ijcsmb.2017.03.555621
S. R.
{"title":"MicroRNA: A Major Key in Pain Neurobiology","authors":"S. R.","doi":"10.19080/ijcsmb.2017.03.555621","DOIUrl":"https://doi.org/10.19080/ijcsmb.2017.03.555621","url":null,"abstract":"","PeriodicalId":93190,"journal":{"name":"International journal of cell science & molecular biology","volume":"73 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68373501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-15DOI: 10.19080/IJCSMB.2018.05.555662
W. Onuigbo
{"title":"Translational Cell Carcinoma of the Nose in a Developing Community","authors":"W. Onuigbo","doi":"10.19080/IJCSMB.2018.05.555662","DOIUrl":"https://doi.org/10.19080/IJCSMB.2018.05.555662","url":null,"abstract":"","PeriodicalId":93190,"journal":{"name":"International journal of cell science & molecular biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49287005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-14DOI: 10.19080/IJCSMB.2018.05.555661
Dahryn Trivedi, S. Jana
Author(s): Trivedi, Dahryn; Jana, Snehasis | Abstract: Mitochondrial dysfunction lead to various serious disorders, which are considered as one of the important components related with the aging, such as type-2 diabetes and Alzheimer’s disease. The aim of the present study was to examine the effect of Consciousness Energy Healing based DMEM medium on murine myoblasts (C2C12) cells to evaluate the mitochondrial mass content using 10-N-nonyl acridine orange (NAO) dye assay. The test item (DMEM medium) was divided into two parts, one part received Consciousness Energy Healing Treatment by a renowned Biofield Energy Healer, Dahryn Trivedi and was labeled as the Biofield Energy Treated DMEM group, while the other part did not receive any kind of Treatment and denoted as the untreated DMEM group. The level of mitochondrial mass content was assessed using 10-N-nonyl acridine orange (NAO) dye method. Cell viability of the test items using MTT assay showed 72.32% and 125.32% viable cells in the untreated DMEM and Biofield Energy Treated DMEM groups, respectively suggested a safe and nontoxic profile of the test items. Besides, the mitochondrial mass content in terms of Fluorescence Unit (FU) was significantly (p≤0.05) increased by 81.78% in the Biofield Energy Treated DMEM group compared to the untreated DMEM group. Overall, the experimental data suggested that the Consciousness Energy Healing Based DMEM showed a significant improvement of mitochondrial mass content and results in better thermogenesis with respect to naive DMEM. Thus, an increased level of NAO dye accumulation in muscle cells indicated increased mitochondrial mass content and hence, better thermogenesis. In the present study, results demonstrated that an increased mitochondrial mass content in the cells when treated with The Trivedi Effect®. This indicates that the test sample has the potential to improve thermogenesis, which can be used against various metabolic diseases, such as insulin resistance, type-2 diabetes, and cardiovascular diseases, etc.
作者:Trivedi, Dahryn;摘要线粒体功能障碍导致多种严重的疾病，被认为是与衰老相关的重要组成部分之一，如2型糖尿病和阿尔茨海默病。本研究的目的是研究基于意识能量愈合的DMEM培养基对小鼠成肌细胞(C2C12)细胞的影响，并使用10- n -壬基吖啶橙(NAO)染色法评估线粒体质量含量。测试项目(DMEM介质)分为两部分，一部分接受著名生物场能量治疗师Dahryn Trivedi的意识能量治疗，标记为生物场能量治疗的DMEM组，另一部分不接受任何治疗，标记为未经治疗的DMEM组。采用10- n -壬基吖啶橙(NAO)染色法测定线粒体质量含量。MTT法检测各组DMEM未处理组和生物场能量处理组的细胞存活率分别为72.32%和125.32%，说明各组DMEM安全无毒。以荧光单位(FU)计算，生物场能量处理DMEM组线粒体质量含量较未处理DMEM组显著(p≤0.05)提高81.78%。总的来说，实验数据表明，基于意识能量治疗的DMEM与原始DMEM相比，线粒体质量含量显著提高，产热效果更好。因此，肌细胞中NAO染料积累水平的增加表明线粒体质量含量增加，因此产热作用更好。在本研究中，结果表明，使用the Trivedi Effect®处理后，细胞中线粒体质量含量增加。这表明该测试样品具有改善产热的潜力，可用于治疗各种代谢疾病，如胰岛素抵抗、2型糖尿病、心血管疾病等。
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Pub Date : 2018-10-24DOI: 10.19080/ijcsmb.2018.05.555660
Santosh Kumar
{"title":"Molecular Characterization of Protease Producing Stenotrophomonas and Their Cytotoxic Effects on Cancer Cell Lines","authors":"Santosh Kumar","doi":"10.19080/ijcsmb.2018.05.555660","DOIUrl":"https://doi.org/10.19080/ijcsmb.2018.05.555660","url":null,"abstract":"","PeriodicalId":93190,"journal":{"name":"International journal of cell science & molecular biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44730241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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