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Morphology exploration of pollen using deep learning latent space 基于深度学习潜空间的花粉形态探索
Pub Date : 2022-12-01 DOI: 10.1088/2633-1357/acadb9
J. Grant-Jacob, M. Zervas, B. Mills
The structure of pollen has evolved depending on its local environment, competition, and ecology. As pollen grains are generally of size 10–100 microns with nanometre-scale substructure, scanning electron microscopy is an important microscopy technique for imaging and analysis. Here, we use style transfer deep learning to allow exploration of latent w-space of scanning electron microscope images of pollen grains and show the potential for using this technique to understand evolutionary pathways and characteristic structural traits of pollen grains.
花粉结构的进化取决于当地的环境、竞争和生态。由于花粉颗粒一般为10-100微米大小,具有纳米级的亚结构,因此扫描电子显微镜是一种重要的成像和分析显微镜技术。在这里,我们使用风格迁移深度学习来探索花粉粒扫描电镜图像的潜在w空间,并展示了使用该技术了解花粉粒进化途径和特征结构特征的潜力。
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引用次数: 0
The infection and recovery periods of the 2022 outbreak of monkey-pox virus disease 2022年猴痘病毒病爆发的感染和恢复期
Pub Date : 2022-11-21 DOI: 10.1088/2633-1357/aca4ad
Marwan Al-Raeei
The Monkey-pox virus disease (MPXD) is a type of the pox disease similar to the smallpox disease. This disease produces rashes with lesions on the skin. The MPXD is an endemic in some countries of Africa, however, a recent outbreak of this disease started to appear in some countries, such as the United Kingdom, Spain, Greece, Portugal, Australia, Brazil, and the United States. Lots of indicators are employed for forecasting the 2022 outbreak of the MPXD such as the infection period, the recovery period, the force of infection, the incubation period of the disease…..etc. The aim of this study is finding the infection period, and the recovery period of the 2022 outbreak of the monkey-pox virus disease in two countries: the United States, and Spain. We apply the simulation and analytical methods on a simple epidemic model, which is the SIRD infectious disease model, for finding the previous periods. We found that the infection period of the recent outbreak of the MPXD varies from 10 days to 20 days, while we found that the recovery periods of the recent outbreak of the MPXD varies from 15 days to 30 days. Besides, we found that the average value of the infection period of the 2022 outbreak of the MPXD is about two weeks, and the average value of the recovery period of the 2022 outbreak of the MPXD is about three weeks. The analytical solution with the simulation algorithm which we used in this study can be expanded for other forecasting parameters of the MPXD, and also for multiple countries.
猴痘病毒病(MPXD)是一种类似于天花的天花疾病。这种疾病会在皮肤上产生带有损伤的皮疹。MPXD是非洲一些国家的地方病,然而,最近在一些国家开始出现这种疾病的爆发,如英国、西班牙、希腊、葡萄牙、澳大利亚、巴西和美国。许多指标用于预测2022年MPXD的爆发,如感染期、恢复期、感染力、疾病潜伏期…。。等。本研究的目的是找出美国和西班牙这两个国家2022年爆发猴痘病毒病的感染期和恢复期。我们将模拟和分析方法应用于一个简单的流行病模型,即SIRD传染病模型,以寻找以前的时期。我们发现,最近爆发的MPXD的感染期从10天到20天不等,而我们发现最近爆发的MPCD的恢复期从15天到30天不等。此外,我们发现2022年MPXD爆发的感染期平均值约为两周,2022年MPXD爆发的恢复期平均值为三周。我们在本研究中使用的模拟算法的分析解可以扩展到MPXD的其他预测参数,也可以扩展到多个国家。
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引用次数: 1
Generated datasets from dynamic reproduction of projectiles in ballistic environments for advanced research (DROPBEAR) testbed 为高级研究(DROPBEAR)试验台从弹道环境中弹丸的动态再现生成数据集
Pub Date : 2022-11-07 DOI: 10.1088/2633-1357/aca0d2
Matthew Nelson, S. Laflamme, Chao Hu, A. Moura, Jonathan Hong, Austin Downey, P. Lander, Yang Wang, Erik Blasch, J. Dodson
High-rate dynamics occur when a system’s acceleration is larger than 100 gn over durations less than 100 ms. Structural health monitoring algorithms must be created for high-rate dynamic systems to maximize safety and minimize economic losses. There is a need to evaluate these algorithms for precision and accuracy prior to real-world implementation. An experimental testbed was created to simulate large-magnitude events while maintaining repeatability to accurately and robustly assess various structural health monitoring algorithms’ capability to monitor high-rate dynamic systems. All previous datasets created on the experimental testbed are discussed, examining various sensor setups, excitations, and boundary condition changes to properly simulate near-high-rate events and provide robust experimental data to evaluate structural health monitoring algorithms.
当系统加速度大于100gn,持续时间小于100ms时,就会出现高速率动力学。为了使高速率动态系统的安全性最大化和经济损失最小化,必须创建结构健康监测算法。在现实世界实现之前,需要评估这些算法的精度和准确性。建立了一个实验测试平台来模拟大震级事件,同时保持可重复性,以准确、稳健地评估各种结构健康监测算法监测高速率动态系统的能力。讨论了之前在实验测试台上创建的所有数据集,检查了各种传感器设置、激励和边界条件变化,以正确模拟近高速率事件,并提供可靠的实验数据来评估结构健康监测算法。
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引用次数: 2
Genome analysis of a plastisphere-associated Oceanimonas sp. NSJ1 sequenced on Nanopore MinION platform 在Nanopore Mineion平台上测序的一种与质体相关的Oceanimonas sp.NSJ1的基因组分析
Pub Date : 2022-10-07 DOI: 10.1088/2633-1357/ac986e
Nirupama Saini, P. Bhadury
Oceanimonas sp. NSJ1 was isolated from macroplastic debris collected previously from Junput, an intertidal beach, facing the northeast coastal Bay of Bengal of the Northern Indian Ocean. The genome of this isolate is closely related to Oceanimonas doudoroffii with a genome size of 3.56 Mbp. The genome annotation confirmed the presence of 5919 total genes, out of which 5809 were CDSs (coding sequences) and all are protein-coding. The genome codes for 110 RNA with 25 rRNA, 84 tRNA (transfer RNA), and one tmRNA (transfer-messenger RNA). Analyses of the annotated genome of Oceanimonas sp. NSJ1 revealed the presence of enzymes involved in the degradation of polycyclic aromatic hydrocarbons. The presence of phthalate 4,5-dioxygenase oxygenase reductase subunit pht2 within the genome also highlights the novelty of this isolate and future functional potential for studying phthalate degradation in marine environment.
Oceanimonas sp.NSJ1是从之前从Junput收集的大塑料碎片中分离出来的,Junput是一个潮间带海滩,面向北印度洋东北海岸孟加拉湾。该分离株的基因组大小为3.56Mbp,与杜多罗菲氏大洋藻(Oceanimonas doudoroffii)亲缘关系密切。基因组注释证实了5919个总基因的存在,其中5809个是CDSs(编码序列),并且都是蛋白质编码。基因组编码110个RNA,其中25个rRNA、84个tRNA(转移RNA)和一个tmRNA(转移信使RNA)。对Oceanimonas sp.NSJ1的注释基因组的分析揭示了参与多环芳烃降解的酶的存在。基因组中邻苯二甲酸盐4,5-二氧合酶加氧酶还原酶亚基pht2的存在也突出了该分离物的新颖性和未来研究邻苯二酸盐在海洋环境中降解的功能潜力。
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引用次数: 0
Prediction of malignant transformation in oral epithelial dysplasia using machine learning. 利用机器学习预测口腔上皮发育不良的恶性转化。
Pub Date : 2022-09-01 Epub Date: 2022-10-07 DOI: 10.1088/2633-1357/ac95e2
James Ingham, Caroline I Smith, Barnaby G Ellis, Conor A Whitley, Asterios Triantafyllou, Philip J Gunning, Steve D Barrett, Peter Gardener, Richard J Shaw, Janet M Risk, Peter Weightman

A machine learning algorithm (MLA) has been applied to a Fourier transform infrared spectroscopy (FTIR) dataset previously analysed with a principal component analysis (PCA) linear discriminant analysis (LDA) model. This comparison has confirmed the robustness of FTIR as a prognostic tool for oral epithelial dysplasia (OED). The MLA is able to predict malignancy with a sensitivity of 84 ± 3% and a specificity of 79 ± 3%. It provides key wavenumbers that will be important for the development of devices that can be used for improved prognosis of OED.

一种机器学习算法(MLA)被应用于傅立叶变换红外光谱(FTIR)数据集,该数据集之前曾用主成分分析(PCA)线性判别分析(LDA)模型进行过分析。这一比较证实了傅立叶变换红外光谱作为口腔上皮发育不良(OED)预后工具的稳健性。MLA 预测恶性肿瘤的灵敏度为 84 ± 3%,特异度为 79 ± 3%。它提供的关键波数对于开发可用于改善 OED 预后的设备非常重要。
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引用次数: 0
Voltammetric investigation on iron-(nickel-)sulfur surface under conditions for the emergence of life 生命出现条件下铁(镍)硫表面的伏安研究
Pub Date : 2022-06-17 DOI: 10.1088/2633-1357/ac79e7
T. Altair, D. Galante, H. Varela
Iron (II) sulfide minerals have gained attention in the last decades due to their relevance in hypotheses for the emergence of life on the early Earth around 4 billion years ago. In the submarine vent theory, it has been proposed that those minerals, especially mackinawite, had a key role in prebiotic processes. Those are estimated to be present in a natural electrochemical setting, analogous to a chemiosmotic one, formed in the interface between the early ocean and the interior of the alkaline hydrothermal systems, the early vent-ocean interface. To evaluate this and other hypotheses, voltammetric studies were performed to better understand the electrochemical behavior of minerals under conditions analogous to the vent-ocean interface. The preliminary results presented here indicate that, in the potential range estimated to exist in that interface, mackinawite can transition to other mineral phases and may posibly coexist with other minerals, resulting from its oxidation. This can create a local chemical diversity. In addition, it has been tested a protocol for Ni incorporation in mackinawite structure, resulting in a surface that showed an interesting behavior in the presence of CO2, although definitive experiments showed necessary for a deeper comprehension of that behavior. Overall, the results are consistent with previous results on electrocatalytical properties of Fe-Ni-S materials for CO2 reduction, and also could lead to the emergence of a protometabolism on early Earth.
铁(II)硫化物矿物在过去的几十年里受到了人们的关注,因为它们与40亿年前地球早期生命出现的假设有关。在海底喷口理论中,有人提出这些矿物质,特别是mackinawite,在益生元过程中起着关键作用。据估计,它们存在于自然的电化学环境中,类似于化学渗透环境,形成于早期海洋和碱性热液系统内部的界面,即早期的喷口-海洋界面。为了评估这一假设和其他假设,进行了伏安研究,以更好地了解类似于喷口-海洋界面条件下矿物的电化学行为。初步结果表明,在该界面估计存在的电位范围内,mackinawite可以过渡到其他矿物相,并可能因氧化而与其他矿物共存。这可以创造当地的化学多样性。此外,它还测试了一种将Ni掺入mackinawite结构的方案,结果表明,在二氧化碳存在的情况下,表面表现出有趣的行为,尽管明确的实验表明需要更深入地理解这种行为。总的来说,这一结果与之前关于Fe-Ni-S材料对CO2还原的电催化性能的结果一致,并且也可能导致早期地球上原代谢的出现。
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引用次数: 0
Preliminary studies on soil radon activity at geothermal hotspot of Bakreswar-Tantloi Bakreswar-Tantloi地热热点土壤氡活性的初步研究
Pub Date : 2022-06-14 DOI: 10.1088/2633-1357/ac78ac
Arkajit Aich
Bakreswar-Tantloi region of India, besides being a geothermally active region, also harbours a high amount of Radon in soil-gas and groundwater of the region. Measurement of Radon-content across the region is an active area of research aimed to properly understand the geothermal potential of the region, the study of local geology and to determine the risks of Radon exposure in the area. In an aim to evaluate the Radon potential of this important geothermal hub, we have done a pilot study on the region by doing spot measurements of Soil-Radon activity in 41 specific geographic locations spread out across the region of interest which will act as groundwork for further in-depth analysis. The measurements showed that Radon activity varies widely in the region with the Radon activity ranging from 1 kBqm−3 to 52 kBqm−3 across the measured locations. Mean Radon activity across the measured locations was found to be 16 kBqm−3 with standard deviation of the sample set of 14 kBqm−3. Seven locations recorded Radon activity to be greater than standard deviation from average while four locations recorded Radon activity greater than twice the standard deviation from average.
印度Bakreswar Tantloi地区除了是一个地热活动区外,该地区的土壤气体和地下水中也含有大量的氡。测量整个地区的氡含量是一个活跃的研究领域,旨在正确了解该地区的地热潜力,研究当地地质,并确定该地区氡暴露的风险。为了评估这一重要地热枢纽的氡潜力,我们对该地区进行了试点研究,对分布在感兴趣地区的41个特定地理位置的土壤氡活动进行了现场测量,这将为进一步深入分析奠定基础。测量结果表明,该地区的氡活性变化很大,测量位置的氡活性范围为1 kBqm−3至52 kBqmm−3。测量位置的平均氡活度为16 kBqm−3,样本集的标准偏差为14 kBqmm−3。七个地点记录的氡活动大于平均值的标准偏差,而四个地点的氡活动则大于平均值标准偏差的两倍。
{"title":"Preliminary studies on soil radon activity at geothermal hotspot of Bakreswar-Tantloi","authors":"Arkajit Aich","doi":"10.1088/2633-1357/ac78ac","DOIUrl":"https://doi.org/10.1088/2633-1357/ac78ac","url":null,"abstract":"Bakreswar-Tantloi region of India, besides being a geothermally active region, also harbours a high amount of Radon in soil-gas and groundwater of the region. Measurement of Radon-content across the region is an active area of research aimed to properly understand the geothermal potential of the region, the study of local geology and to determine the risks of Radon exposure in the area. In an aim to evaluate the Radon potential of this important geothermal hub, we have done a pilot study on the region by doing spot measurements of Soil-Radon activity in 41 specific geographic locations spread out across the region of interest which will act as groundwork for further in-depth analysis. The measurements showed that Radon activity varies widely in the region with the Radon activity ranging from 1 kBqm−3 to 52 kBqm−3 across the measured locations. Mean Radon activity across the measured locations was found to be 16 kBqm−3 with standard deviation of the sample set of 14 kBqm−3. Seven locations recorded Radon activity to be greater than standard deviation from average while four locations recorded Radon activity greater than twice the standard deviation from average.","PeriodicalId":93771,"journal":{"name":"IOP SciNotes","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48756008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Effect of different fixatives on yield of DNA from human fecal samples 不同固定剂对人粪便样品DNA产率的影响
Pub Date : 2022-05-05 DOI: 10.1088/2633-1357/ac6d2e
Gauraw Kumar, P. Bhadury
Fixation and transportation of human fecal samples is often difficult in geographically remote locations due to unavailability of options for immediate freezing. In this study effectiveness of five different chemical fixatives were evaluated on human fecal samples including for supernatant using RNAprotect® Bacteria Reagent (Qiagen), 95% ethanol, acetone, TRIzol® and a mixture of all these fixatives, in addition to immediate freezing. DNA was extracted from the fecal samples using QIAamp® Fast DNA Stool Mini Kit as well as quality and yield of extracted DNA was monitored for a period of 30 days. It was found that except TRIzol®, all other preservatives showed good DNA quality and yield for a period of one month based on agarose gel electrophoresis, Nanodrop and Qubit fluorometric measurements. It was also found that supernatant of fecal sample fixed with RNAprotect Bacteria Reagent gave reliable DNA yield in comparison to other various fixatives. The study also revealed that quality and yield of DNA from fecal samples fixed in acetone were very promising since it is a cost-effective fixative. Overall, the study shows future applicability for downstream DNA analyses of the RNAprotect® Bacteria Reagent, 95% ethanol, acetone, and a mixture of all these fixatives for fixing human fecal samples to be collected from geographically remote locations or in regions where available resources are largely limited.
在地理位置偏远的地方,由于无法选择立即冷冻,人类粪便样本的固定和运输通常很困难。在这项研究中,除了立即冷冻外,还评估了五种不同化学固定剂对人类粪便样本的有效性,包括使用RNAprotect®细菌试剂(Qiagen)、95%乙醇、丙酮、TRIzol®和所有这些固定剂的混合物的上清液。使用QIAamp®Fast DNA Stool Mini试剂盒从粪便样本中提取DNA,并监测提取DNA的质量和产量30天。根据琼脂糖凝胶电泳、Nanodrop和Qubit荧光测量,发现除TRIzol®外,所有其他防腐剂在一个月内都显示出良好的DNA质量和产量。还发现,与其他各种固定剂相比,用RNAprotect细菌试剂固定的粪便样品的上清液提供了可靠的DNA产量。该研究还表明,固定在丙酮中的粪便样本的DNA的质量和产量非常有希望,因为它是一种具有成本效益的固定剂。总的来说,这项研究表明,RNAprotect®细菌试剂、95%乙醇、丙酮和所有这些固定剂的混合物未来适用于下游DNA分析,用于固定从地理偏远地区或可用资源有限的地区收集的人类粪便样本。
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引用次数: 0
Generating images of hydrated pollen grains using deep learning 使用深度学习生成水合花粉粒的图像
Pub Date : 2022-04-14 DOI: 10.1088/2633-1357/ac6780
J. Grant-Jacob, M. Praeger, R. Eason, B. Mills
Pollen grains dehydrate during their development and following their departure from the host stigma. Since the size and shape of a pollen grain can be dependent on environmental conditions, being able to predict both of these factors for hydrated pollen grains from their dehydrated state could be beneficial in the fields of climate science, agriculture, and palynology. Here, we use deep learning to transform images of dehydrated Ranunculus pollen grains into images of hydrated Ranunculus pollen grains. We also then use a deep learning neural network that was trained on experimental images of different genera of pollen grains to identify the hydrated pollen grains from the generated transformed images, to test the accuracy of the image generation neural network. This pilot work demonstrates the first steps needed towards creating a general deep learning-based rehydration model that could be useful in understanding and predicting pollen morphology.
花粉粒在发育过程中以及离开寄主柱头后会脱水。由于花粉粒的大小和形状可能取决于环境条件,因此能够从脱水状态预测水合花粉粒的这两个因素在气候科学、农业和孢粉学领域都是有益的。在这里,我们使用深度学习将脱水毛茛花粉粒的图像转换为水合毛茛花粉粒的图像。然后,我们还使用在不同属花粉粒的实验图像上训练的深度学习神经网络,从生成的转换图像中识别水合花粉粒,以测试图像生成神经网络的准确性。这项试点工作展示了创建一个基于深度学习的通用再水合模型所需的第一步,该模型可能有助于理解和预测花粉形态。
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引用次数: 3
Automatic rule-based generation of spinal cord connectome model for a neuro-musculoskeletal limb in-silico 基于规则的神经-肌肉-骨骼肢体脊髓连接体模型的自动生成
Pub Date : 2022-02-24 DOI: 10.1088/2633-1357/ac585e
M. V. Pithapuram, M. Raghavan
Studying spinal interactions with muscles has been of great importance for over a century. However, with surging spinal-related movement pathologies, the need for computational models to study spinal pathways is increasing. Although spinal cord connectome models have been developed, anatomically relevant spinal neuromotor models are rare. However, building and maintaining such models is time-consuming. In this study, the concept of the rule-based generation of a spinal connectome was introduced and lumbosacral connectome generation was demonstrated as an example. Furthermore, the rule-based autogenerated connectome models were synchronized with lower-limb musculoskeletal models to create an in-silico testbed. Using this setup, the role of the autogenic Ia-excitatory pathway in controlling the ankle angle was tested.
一个多世纪以来,研究脊柱与肌肉的相互作用一直具有重要意义。然而,随着脊柱相关运动病理学的激增,对研究脊柱路径的计算模型的需求越来越大。尽管脊髓连接体模型已经开发出来,但与解剖学相关的脊髓神经运动模型却很少见。然而,构建和维护这样的模型非常耗时。在本研究中,引入了基于规则的脊椎连接体生成的概念,并以腰骶连接体生成为例进行了演示。此外,基于规则的自动生成连接体模型与下肢肌肉骨骼模型同步,以创建一个计算机试验台。使用这种装置,测试了自发Ia兴奋通路在控制踝关节角度中的作用。
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引用次数: 0
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