Food research international (Ottawa, Ont.)最新文献

The prevention of coffee fraud through the use of digital and intelligence-based technologies is an analytical challenge because depending on the adulterant, visual inspection is unreliable in roasted and ground coffee due to the similarity in color and texture of the materials used. In this work, a 3D-printed apparatus for smartphone image acquisiton is proposed. The digital images are used to authenticate the geographical origin of indigenous canephora coffees produced at Amazon region, Brazil, against canephora coffees from Espírito Santo, Brazil, and to capture the adulteration of indigenous samples. The results evidenced that the technology is favorable to identify the geographical origin and adulteration with multiple substances using smartphone technology. Pure coffees were adulterated with arabica coffee, spent coffee ground, low-quality Canephora coffee, coffee husks, açaí, corn, and soybean in increasing proportions of 10, 20, 30, 40, 50, 60, and 70 %. These adulterants were roasted and grounded similarly to Canephora coffees to mimetize a highly-sophisticated fraud. The images were converted into Red-Green-Blue (RGB) fingerprinting and used as analytical response to construct Data-Driven Soft Independent Modeling of Class Analogy (DD-SIMCA) models. A total of 95 % of all target and non-target samples in the test set were correctely identified, aiding producers and consumers in ensuring accurate labeling and supporting traditional communities economically and culturally. Smartphone-based method demonstrated potential to innovate the coffee safety control representing a new analytical tecnology.

The objective of this study was to investigate the immunomodulatory effects of whey protein isolate (WPI)-galacto-oligosaccharide conjugates following dynamic high-pressure microfluidics pretreatment (DHPM) in cyclophosphamide-induced immunosuppressed mice. DHPM facilitated the conjugation of WPI and galacto-oligosaccharide, and inhibited the generation of fluorescent advanced glycation end products (AGEs) and pentosidine. The conjugates demonstrated a significant immune recovery effect on CTX-induced immunosuppressed mice, as evidenced by the enhancement of IgG antibody levels (from 3.5 to 4.1) and the reduction of the levels of immunosuppressive effector factors TGF-β (from 148.1 to 111.2) and IFN-γ (from 34.4 to 17.9). Furthermore, the conjugates exhibited a notable ability to repair histological lesion in the spleen of CTX-induced immunosuppressed mice. Spleen transcriptomics revealed that the Marco, Klrc3 and Cd209b genes were associated with the immune enhancement activity of the conjugates. Metabolomic analysis identified arginine biosynthesis, sphingolipid metabolism, alanine, aspartate and glutamate metabolism, and phenylalanine, tyrosine and tryptophan biosynthesis as key pathways in the immune enhancement activity of the conjugates. Metabolomics combined with transcriptomics indicated the importance of macrophage activation in the restoration of immunosuppressed mice's immunity by the conjugates. Therefore, the improvement in immunity observed with WPI-galacto-oligosaccharide conjugates may be related to the activation of macrophages.

This study investigates the comprehensive effects of extraction parameters, freeze-drying, and formulation on the chemical composition, colour properties, antioxidant and anti-inflammatory activities, and reactive oxygen species (ROS) generation of blackberry (BB) and elderberry (EB) extracts, as well as their incorporation into gummies. Using response surface methodology, optimal extraction conditions were identified: BB extracts showed optimal results at 325 W and 7.5 min, while EB extracts were optimal at 400 W and 5 min. The EB extracts consistently exhibited higher total phenolic content, total anthocyanin content, and antioxidant capacity than the BB extracts. Over 120 min, BB extracts demonstrated superior antioxidant potential to mitigate human plasma lipid oxidation. Both extracts displayed pH-dependent colour variations and antioxidant capacities, with EB extracts showing greater stability across a broader pH range. Freeze-drying effectively preserved antioxidant capacity, with EB extracts maintaining higher values than BB extracts. In a cellular model of oxidative stress using THP-1, both extracts were non-cytotoxic and reduced intracellular ROS generation, with EB extracts also more effectively inhibiting IL-6 secretion. When incorporated into gummies, these extracts resulted in higher phenolic and anthocyanin content than commercial counterparts, with EB gummies demonstrating superior antioxidant capacity. Sensory evaluations indicated no significant differences in taste, texture, or overall acceptability among the gummy formulations, though colour preferences tended to favour commercial gummies. This study addresses a gap by providing detailed chemical, biological, and sensory assessments of BB and EB extracts in food applications.

The hepatoprotective potential of tomato juice (TJ) has been reported in chronic liver models, and its potential prebiotic properties may be key to its preventative effects. However, the mechanistic role of the gut microbiota and its derived metabolites in ameliorating nonalcoholic steatohepatitis (NASH) via TJ remains unclear. In this study, we explored how TJ regulates gut microbiota and succinic acid (SA) to restore intestinal barrier function and thus suppress NASH progression. TJ supplementation effectively reduced serum lipid concentrations, alleviated endotoxin levels, and suppressed activation of the endotoxin-TLR4-NF-κB pathway in methionine-choline-deficient (MCD) diet-induced NASH mice. TJ restored the MCD diet-induced gut microbiota dysbiosis, increased the abundance of short-chain fatty acid and SA-producing bacteria (Bifidobacterium, Ileibacterium, Odoribacter, and Parasutterella) and enhanced the expression of intestinal barrier-associated proteins (E-cadherin, Claudin-1, MUC-2, and ZO-1). The hepatoprotective and enteroprotective effects of TJ were abolished in an antibiotic-treated mouse model, underscoring the pivotal role of the gut microbiota in the beneficial effects of TJ on NASH. Fecal metabolomics demonstrated that TJ significantly upregulated the tricarboxylic acid cycle, pyruvate metabolism, and butanoate metabolism pathways, increasing levels of butyric acid (BA) and SA-metabolites associated with reduced hepatic steatosis and intestinal damage. We further found that the physiological concentration of SA, rather than BA, could reduce pro-inflammatory cytokines (TNF-α and IL-6) levels and enhance mucin proteins and tight junction markers in the LPS-induced colon cell line LS174T. This study uncovers new mechanisms by which TJ prevents NASH, highlighting the potential of TJ and SA as effective dietary supplements for patients with chronic liver diseases.

Black Soldier Fly larvae (BSFL) are a promising and sustainable alternative to obtain proteins. Due to their high growth rate and ability to use different substrates as feeding stocks, BSFL can be also used to valorize food waste. Thus, the aim of this research was to unravel the potential use of Spent Coffee Grounds (SCG) and blood meal alone or mixed as feedstocks for BSFL and the nutritional changes for BSFL meal, especially after simulated human in vitro digestion and fermentation. Chicken feed was used as a control. Chicken feed showed the highest BSFL growth (P < 0.05) compared with blood meal and the mix made of blood meal and SCG; the latter caused the lowest growth. The meal obtained from BSFL fed with blood meal had the highest protein content, as well as the highest levels of short chain fatty acids (SCFAs) produced after in vitro fermentation by the human gut microbiota. On the other hand, the meal from larvae fed with SCG showed higher antioxidant capacity than the others in the DPPH, FRAP and ABTS assays. The digestibility of macronutrients, release of antioxidant capacity and production of SCFAs of the BSFL meal were improved when using these substrates, compared to chicken feed.

Propolis is a natural immunomodulator with anticancer activity. This study investigated the immunomodulatory mechanism and anti-tumor activity of supercritical CO₂ extracts of propolis (SEP) in tumor-bearing immunosuppression mice. We used cyclophosphamide (CTX) to construct the immunosuppressive mice model and then inoculated them with CT26 cells to build the CT26 tumor-bearing immunosuppression mice model. Upon treatment with SEP, tumor proliferation in mice was markedly suppressed, with tumor volumes decreasing from 1881.43 mm³ to 1049.95 mm³ and weights reducing from 2.07 g to 1.13 g. Concurrently, the immune system recovered well, and the spleen and thymus indexes increased significantly. The total T lymphocyte (CD3⁺ T cell) contents in the spleen and blood recovered from 11.88 % to 21.19 % and 15.32 % to 22.19 %, respectively. In addition, the CD4⁺ /CD8⁺ ratio has returned to a healthy level, 3.12 in the spleen and 5.42 in the blood. The levels of IL-1β, IL-6, and TNF-α were increased by 2.17, 2.76, and 7.15 times in the spleen, 2.76, 1.92, and 3.02 times in the serum. Moreover, the western blot results showed that SEP treatment increased the expression of toll-like receptor 4 (TLR4) and the phosphorylation of p38, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p65. These results indicated that SEP activated the immune activity of RAW 264.7 macrophages through the TLR4-mitogen-activated protein kinase (MAPK)/nuclear factor kappa B (NF-κB) signaling pathway to exert immunomodulatory function and inhibit tumor proliferation. This study facilitated the further application of SEP as a potential immunomodulatory and anti-tumor functional food.

Insects are a rich source of proteins and are produced in systems that have lower environmental impact. As an alternative protein source, they can be consumed directly or used as an ingredient in other formulations. Recently, there has been growing interest in utilizing insect proteins as a substrate to obtain bioactive peptides as well as in investigating the maintenance of their biological properties under physiological conditions. This study aimed to evaluate the impact of simulated digestion on the bioactive properties of protein hydrolysates from black crickets (Gryllus assimilis). Following simulated digestion of the hydrolysate obtained through the application of Flavourzyme, the scavenging activities of ABTS and DPPH radicals, and ferric reducing antioxidant power (FRAP) increased by approximately 17 %, 246 %, and 173 %, respectively. For the hydrolysate obtained using the binary combination of Flavourzyme/Neutrase, the inhibitory activities of α-amylase and α-glucosidase after digestion were 47.87 % and 12.73 %, respectively, not significantly (p > 0.05) different from non-digested hydrolysates. The angiotensin-converting enzyme (ACE) inhibitory activity of the sample hydrolyzed with Flavourzyme/Alcalase proteases was 42.22 %, but this property was completely lost after in vitro digestion. Untargeted proteomic analysis allowed the identification of 22 peptides in the <3 kDa fraction of the digested black cricket protein. The LPPLP sequence was considered potentially bioactive for all activities tested in silico.

Patatin is an acidic protein found in potatoes that is commonly used in food and pharmaceutical industries due to its excellent emulsifying and gelation abilities. Pichia pastoris is widely used as a host for recombinant protein production because it can incorporate post-translational modifications. In this study, a patatin titre of 2189.8 mg/L was achieved in a 5 L bioreactor using P. pastoris GS115 with signal peptide mutation, dual promoter construction, co-expression of chaperone proteins and optimised fermentation. To enhance the application of recombinant patatin in the food processing field, the level of N-glycosylation was elevated by genetic engineering. Properties of natural patatin, recombinant patatin and patatin^L109T (N-glycosylated modified patatin) were investigated including foaming, hydrophobicity and emulsifying abilities. The functional properties of recombinant patatin were enhanced by introducing N-glycosylation, which also improved the water-holding capacity of its gel. The patatin^L109T gel exhibited superior elasticity and water retention properties. The findings provide valuable insight and serve as a reference for the utilisation of recombinant patatin. The established enhancement strategy could be applied to other recombinant proteins.

This study aimed to assess the impact of adding strawberry and acerola jam, along with Limosilactobacillus mucosae CNPC007, on the technological, nutritional, bioactive, and microbiological properties of Greek-style goat yogurt. Six yogurt formulations were developed: without and with the addition of L. mucosae CNPC007 (CY and PY, respectively), and with 10 % and 15 % jam (CY10, CY15, PY10, and PY15, respectively). The inclusion of jam enriched the yogurt with phenolic compounds and significantly enhanced antioxidant activity, as measured by FRAP and ABTS assays. The highest values were observed after 28 days of storage in the PY15 formulation (0.177 ± 0.01 and 3.43 ± 0.01 µmol TEAC/g, respectively), compared to CY (0.013 ± 0.01 and 0.19 ± 0.01 µmol TEAC/g, respectively) and PY (0.010 ± 0.01 and 0.23 ± 0.01 µmol TEAC/g, respectively). This increase was likely driven by the presence of anthocyanins and flavonoids in the jam, as indicated by heatmap correlation analysis. DPI and EPI were also influenced by the addition of jam and L. mucosae CNPC007, with EPI increasing in the PY10 and PY15 formulations, reaching approximately 40 % after 28 days. The incorporation of jam resulted in a decrease in the L* (<90) and an increase in the b* (>14) color parameters. Additionally, jam-enriched formulations exhibited higher syneresis and lower water retention capacity (WRC) throughout storage compared to control formulations, with average syneresis exceeding 26 % and WRC falling below 75 % after 28 days. In general, all yogurt formulations showed a reduction in lactose, an increase in glucose and galactose, and the production of lactic acid during storage. The lower lactose content observed after 28 days of storage in the PY (0.84 ± 0.01 g/100 g), PY10 (0.82 ± 0.01 g/100 g), and PY15 (0.98 ± 0.01 g/100 g) formulations indicates active sugar metabolism by L. mucosae CNPC007. All formulations met microbiological safety standards, confirming their suitability for consumption. Formulations containing L. mucosae CNPC007 showed viable cell counts exceeding the minimum recommended to produce health benefits (>7 log CFU/g) throughout the 28-day refrigerated storage and after in vitro digestion. These findings underscore the potential of combining tropical fruit jams with probiotics to develop a multifunctional, value-added yogurt product that delivers substantial health benefits to consumers.

Aiming to address the issue of stored grain pests easily breeding during the process of dried fruits in Xinjiang, this study proposes a method and a device for killing raisin parasitic eggs based on a high-voltage pulsed electric field. A one-way test and a Box-Behnken central combination test were conducted to investigate the effects of high-voltage pulsed electric field strength and frequency on the unhatched rate and larval survival rate of Plodia interpunctella eggs on raisin surfaces. The experimental results were qualitatively and quantitatively analyzed using biooptical microscope observation and incubation at constant temperature and humidity post-treatment. The findings revealed that with an output voltage of 22.8 kV, the delivery speed of 0.024 m/s, and the electric field frequency of 3.8 Hz, the unhatched rate of the eggs was 68.14 % while the survival rate of the larvae was 20.36 %. These results can provide new insights for both theoretical development and system implementation regarding the use of high voltage pulsed electric fields for eliminating raisin surface eggs, as well as providing valuable academic references for field crop diseases and pests control strategies.