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Ectomycorrhizal fungi: Potential guardians of terrestrial ecosystems. 外生菌根真菌:陆地生态系统的潜在守护者。
IF 4.5 Q1 MICROBIOLOGY Pub Date : 2024-07-31 eCollection Date: 2024-09-01 DOI: 10.1002/mlf2.12127
Wenchen Song
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引用次数: 0
HPClas: A data‐driven approach for identifying halophilic proteins based on catBoost HPClas:基于 catBoost 的数据驱动型嗜卤蛋白质识别方法
IF 4.5 Q1 MICROBIOLOGY Pub Date : 2024-07-20 DOI: 10.1002/mlf2.12125
Shantong Hu, Xiao-Yong Wang, Zhikang Wang, Menghan Jiang, Shihui Wang, Wenya Wang, Jiangning Song, Guimin Zhang
Halophilic proteins possess unique structural properties and show high stability under extreme conditions. This distinct characteristic makes them invaluable for application in various aspects such as bioenergy, pharmaceuticals, environmental clean‐up, and energy production. Generally, halophilic proteins are discovered and characterized through labor‐intensive and time‐consuming wet lab experiments. In this study, we introduce the Halophilic Protein Classifier (HPClas), a machine learning‐based classifier developed using the catBoost ensemble learning technique to identify halophilic proteins. Extensive in silico calculations were conducted on a large public dataset of 12,574 samples and HPClas achieved an area under the receiver operating characteristic curve (AUROC) of 0.844 on an independent test set of 200 samples. The source code and curated dataset of HPClas are publicly available at https://github.com/Showmake2/HPClas. In conclusion, HPClas can be explored as a promising tool to aid in the identification of halophilic proteins and accelerate their application in different fields.
嗜卤蛋白质具有独特的结构特性,在极端条件下表现出高度稳定性。这一显著特点使它们在生物能源、制药、环境清洁和能源生产等各方面的应用变得非常宝贵。一般来说,嗜卤蛋白质的发现和表征需要通过耗费大量人力和时间的湿实验室实验来完成。在本研究中,我们介绍了嗜卤蛋白质分类器(HPClas),这是一种基于机器学习的分类器,采用 catBoost 集合学习技术开发,用于识别嗜卤蛋白质。在一个包含12574个样本的大型公共数据集上进行了广泛的硅计算,在一个包含200个样本的独立测试集上,HPClas的接收者操作特征曲线下面积(AUROC)达到了0.844。HPClas 的源代码和数据集可在 https://github.com/Showmake2/HPClas 上公开获取。总之,HPClas 是一种很有前途的工具,可以帮助鉴定嗜卤蛋白质并加速其在不同领域的应用。
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引用次数: 0
Cofitness network connectivity determines a fuzzy essential zone in open bacterial pangenome. 协同网络连通性决定了开放细菌泛基因组的模糊基本区。
IF 4.5 Q1 MICROBIOLOGY Pub Date : 2024-06-28 eCollection Date: 2024-06-01 DOI: 10.1002/mlf2.12132
Pan Zhang, Biliang Zhang, Yuan-Yuan Ji, Jian Jiao, Ziding Zhang, Chang-Fu Tian

Most in silico evolutionary studies commonly assumed that core genes are essential for cellular function, while accessory genes are dispensable, particularly in nutrient-rich environments. However, this assumption is seldom tested genetically within the pangenome context. In this study, we conducted a robust pangenomic Tn-seq analysis of fitness genes in a nutrient-rich medium for Sinorhizobium strains with a canonical open pangenome. To evaluate the robustness of fitness category assignment, Tn-seq data for three independent mutant libraries per strain were analyzed by three methods, which indicates that the Hidden Markov Model (HMM)-based method is most robust to variations between mutant libraries and not sensitive to data size, outperforming the Bayesian and Monte Carlo simulation-based methods. Consequently, the HMM method was used to classify the fitness category. Fitness genes, categorized as essential (ES), advantage (GA), and disadvantage (GD) genes for growth, are enriched in core genes, while nonessential genes (NE) are over-represented in accessory genes. Accessory ES/GA genes showed a lower fitness effect than core ES/GA genes. Connectivity degrees in the cofitness network decrease in the order of ES, GD, and GA/NE. In addition to accessory genes, 1599 out of 3284 core genes display differential essentiality across test strains. Within the pangenome core, both shared quasi-essential (ES and GA) and strain-dependent fitness genes are enriched in similar functional categories. Our analysis demonstrates a considerable fuzzy essential zone determined by cofitness connectivity degrees in Sinorhizobium pangenome and highlights the power of the cofitness network in understanding the genetic basis of ever-increasing prokaryotic pangenome data.

大多数硅学进化研究通常假定,核心基因对细胞功能至关重要,而附属基因则可有可无,尤其是在营养丰富的环境中。然而,这种假设很少在泛基因组背景下进行基因测试。在本研究中,我们对营养丰富的培养基中具有典型开放庞基因组的肉毒杆菌菌株进行了稳健的庞基因组 Tn-seq 分析。结果表明,基于隐马尔可夫模型(HMM)的方法对突变库之间的变化最为稳健,而且对数据量不敏感,优于基于贝叶斯和蒙特卡罗模拟的方法。因此,我们使用 HMM 方法来划分适合度类别。适合度基因分为生长必需基因(ES)、优势基因(GA)和劣势基因(GD),它们在核心基因中富集,而非必需基因(NE)在附属基因中比例过高。与核心 ES/GA 基因相比,附属 ES/GA 基因表现出较低的适应性效应。共效网络中的连接度按照ES、GD和GA/NE的顺序递减。除附属基因外,3284个核心基因中有1599个基因在不同的测试品系中显示出不同的重要程度。在泛基因组核心中,共享的准基本基因(ES 和 GA)和依赖于品系的适生性基因都富集在类似的功能类别中。我们的分析表明,在根瘤菌庞基因组中,共适性连接度决定了一个相当大的模糊必要区,并突出了共适性网络在理解不断增加的原核生物庞基因组数据的遗传基础方面的作用。
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引用次数: 0
Identification of 3-ketocapnine reductase activity within the human microbiota. 鉴定人体微生物群中的 3-Ketocapnine 还原酶活性。
IF 4.5 Q1 MICROBIOLOGY Pub Date : 2024-06-28 eCollection Date: 2024-06-01 DOI: 10.1002/mlf2.12134
Xiaotong Wu, Lukuan Hou, Haili Zhang, Yi Ma, Jufang Wang, Mingwei Cai, Xiaoyu Tang

The microbial synthesis of sulfonolipids within the human body is likely involved in maintaining human health or causing diseases. However, the enzymes responsible for their biosynthesis remain largely unknown. In this study, we identified and verified the role of 3-ketocapnine reductase, the third-step enzyme, in the four-step conversion of l-phosphoserine into sulfobacin B both in vivo and in vitro. This finding builds upon our previous research into sulfonolipid biosynthesis, which focused on the vaginal bacterium Chryseobacterium gleum DSM 16776 and the gut bacterium Alistipes finegoldii DSM 17242. Through comprehensive gene mapping, we demonstrate the widespread presence of potential sulfonolipid biosynthetic genes across diverse bacterial species inhabiting various regions of the human body. These findings shed light on the prevalence of sulfonolipid-like metabolites within the human microbiota, suggesting a potential role for these lipid molecules in influencing the intricate biointeractions within the complex microbial ecosystem of the human body.

微生物在人体内合成的磺脂类很可能与维持人体健康或导致疾病有关。然而,负责其生物合成的酶在很大程度上仍不为人所知。在这项研究中,我们发现并验证了 3-酮巯基还原酶(第三步酶)在体内和体外将 l-磷酸丝氨酸转化为磺胺酸 B 的四步转化过程中的作用。这一发现建立在我们以前对磺脂类生物合成的研究基础之上,以前的研究主要集中在阴道细菌 Chryseobacterium gleum DSM 16776 和肠道细菌 Alistipes finegoldii DSM 17242 上。通过全面的基因图谱绘制,我们证明了潜在的磺脂类生物合成基因广泛存在于栖息在人体不同区域的不同细菌物种中。这些发现揭示了人体微生物群中磺脂类代谢物的普遍性,表明这些脂质分子在影响人体复杂的微生物生态系统中错综复杂的生物相互作用方面具有潜在的作用。
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引用次数: 0
A novel alcohol dehydrogenase in the hyperthermophilic crenarchaeon Hyperthermus butylicus. 高热嗜温性丁香菌(Hyperthermus butylicus)中的一种新型醇脱氢酶。
IF 4.5 Q1 MICROBIOLOGY Pub Date : 2024-06-28 eCollection Date: 2024-06-01 DOI: 10.1002/mlf2.12126
Ching Tse, Kesen Ma

Hyperthermus butylicus is a hyperthermophilic crenarchaeon that produces 1-butanol as an end product. A thermostable alcohol dehydrogenase (ADH) must be present in H. butylicus to act as the key enzyme responsible for this production; however, the gene that encodes the ADH has not yet been identified. A novel ADH, HbADH2, was purified from a cell-free extract of H. butylicus, and its characteristics were determined. The gene that encodes HbADH2 was demonstrated to be HBUT_RS04850 and annotated as a hypothetical protein in H. butylicus. HbADH2 was found to be a primary-secondary ADH capable of using a wide range of substrates, including butyraldehyde and butanol. Butyraldehyde had the highest specificity constant, calculated as k c at/K m, with k cat and apparent K m values of 8.00 ± 0.22 s-1 and 0.59 ± 0.07 mM, respectively. The apparent K m values for other substrates, including ethanol, 1-propanol, 2-propanol, butanol, acetaldehyde, propanal, and acetone, were 4.36 ± 0.42, 4.69 ± 0.41, 3.74 ± 0.46, 2.44 ± 0.30, 1.27 ± 0.18, 1.55 ± 0.20, and 0.68 ± 0.04 mM, respectively. The optimal pH values for catalyzing aldehyde reduction and alcohol oxidation were 6.0 and 9.0, respectively, while the optimal temperature was higher than 90°C due to the increase in enzymatic activity from 60°C to 90°C. Based on its substrate specificity, enzyme kinetics, and thermostability, HbADH2 may be the ADH that catalyzes the production of 1-butanol in H. butylicus. The putative conserved motif sites for NAD(P)+ and iron binding were identified by aligning HbADH2 with previously characterized Fe-containing ADHs.

丁醇嗜热菌(Hyperthermus butylicus)是一种嗜热栗色菌,其最终产物为 1-丁醇。丁醇嗜热菌中必须存在一种可恒温的醇脱氢酶(ADH),它是产生这种产物的关键酶;然而,编码 ADH 的基因尚未确定。我们从丁酸梭菌的无细胞提取物中纯化出了一种新型 ADH--HbADH2,并确定了它的特征。编码 HbADH2 的基因被证明是 HBUT_RS04850,并被注释为丁酸杆菌中的一种假定蛋白。研究发现,HbADH2 是一种初级-次级 ADH,能够使用多种底物,包括丁醛和丁醇。丁醛的特异性常数最高,以 k c at/K m 计算,k cat 和表观 K m 值分别为 8.00 ± 0.22 s-1 和 0.59 ± 0.07 mM。其他底物(包括乙醇、1-丙醇、2-丙醇、丁醇、乙醛、丙醛和丙酮)的表观 K m 值分别为 4.36 ± 0.42、4.69 ± 0.41、3.74 ± 0.46、2.44 ± 0.30、1.27 ± 0.18、1.55 ± 0.20 和 0.68 ± 0.04 mM。催化醛还原和醇氧化的最佳 pH 值分别为 6.0 和 9.0,而最佳温度则高于 90°C,这是因为酶活性从 60°C 升高到 90°C。根据其底物特异性、酶动力学和恒温性,HbADH2 可能是催化丁酸杆菌产生 1-丁醇的 ADH。通过将 HbADH2 与先前表征的含铁 ADH 进行比对,确定了 NAD(P)+ 和铁结合的推定保守基团位点。
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引用次数: 0
Elucidating microbial iron corrosion mechanisms with a hydrogenase-deficient strain of Desulfovibrio vulgaris. 利用氢化酶缺陷的普通脱硫弧菌菌株阐明微生物的铁腐蚀机制。
IF 4.5 Q1 MICROBIOLOGY Pub Date : 2024-06-28 eCollection Date: 2024-06-01 DOI: 10.1002/mlf2.12133
Di Wang, Toshiyuki Ueki, Peiyu Ma, Dake Xu, Derek R Lovley

Sulfate-reducing microorganisms extensively contribute to the corrosion of ferrous metal infrastructure. There is substantial debate over their corrosion mechanisms. We investigated Fe0 corrosion with Desulfovibrio vulgaris, the sulfate reducer most often employed in corrosion studies. Cultures were grown with both lactate and Fe0 as potential electron donors to replicate the common environmental condition in which organic substrates help fuel the growth of corrosive microbes. Fe0 was corroded in cultures of a D. vulgaris hydrogenase-deficient mutant with the 1:1 correspondence between Fe0 loss and H2 accumulation expected for Fe0 oxidation coupled to H+ reduction to H2. This result and the extent of sulfate reduction indicated that D. vulgaris was not capable of direct Fe0-to-microbe electron transfer even though it was provided with a supplementary energy source in the presence of abundant ferrous sulfide. Corrosion in the hydrogenase-deficient mutant cultures was greater than in sterile controls, demonstrating that H2 removal was not necessary for the enhanced corrosion observed in the presence of microbes. The parental H2-consuming strain corroded more Fe0 than the mutant strain, which could be attributed to H2 oxidation coupled to sulfate reduction, producing sulfide that further stimulated Fe0 oxidation. The results suggest that H2 consumption is not necessary for microbially enhanced corrosion, but H2 oxidation can indirectly promote corrosion by increasing sulfide generation from sulfate reduction. The finding that D. vulgaris was incapable of direct electron uptake from Fe0 reaffirms that direct metal-to-microbe electron transfer has yet to be rigorously described in sulfate-reducing microbes.

硫酸盐还原微生物广泛造成黑色金属基础设施的腐蚀。关于它们的腐蚀机制还存在大量争议。我们研究了硫酸盐还原菌(Desulfovibrio vulgaris)对 Fe0 的腐蚀,它是腐蚀研究中最常用的硫酸盐还原菌。培养物以乳酸盐和 Fe0 作为潜在的电子供体,以复制有机底物有助于腐蚀性微生物生长的常见环境条件。在一种 D. vulgaris 氢酶缺陷突变体的培养物中,Fe0 被腐蚀,Fe0 氧化与 H+ 还原成 H2 的过程中,Fe0 损失与 H2 积累的比例为 1:1。这一结果和硫酸盐还原的程度表明,即使在大量硫化亚铁存在的情况下为 D. vulgaris 提供了补充能源,它也无法实现 Fe0 到微生物的直接电子传递。氢化酶缺乏的突变体培养物的腐蚀程度高于无菌对照组,这表明在有微生物存在的情况下,H2的去除并不是增强腐蚀的必要条件。亲本消耗 H2 的菌株比突变菌株腐蚀更多的 Fe0,这可能是由于 H2 氧化与硫酸盐还原耦合,产生了进一步刺激 Fe0 氧化的硫化物。结果表明,微生物增强腐蚀并不需要消耗 H2,但 H2 氧化可通过增加硫酸盐还原产生的硫化物来间接促进腐蚀。发现 D. vulgaris 无法直接从 Fe0 吸收电子再次证明,在硫酸盐还原微生物中,金属与微生物之间的直接电子传递尚未得到严格描述。
{"title":"Elucidating microbial iron corrosion mechanisms with a hydrogenase-deficient strain of <i>Desulfovibrio vulgaris</i>.","authors":"Di Wang, Toshiyuki Ueki, Peiyu Ma, Dake Xu, Derek R Lovley","doi":"10.1002/mlf2.12133","DOIUrl":"10.1002/mlf2.12133","url":null,"abstract":"<p><p>Sulfate-reducing microorganisms extensively contribute to the corrosion of ferrous metal infrastructure. There is substantial debate over their corrosion mechanisms. We investigated Fe<sup>0</sup> corrosion with <i>Desulfovibrio vulgaris</i>, the sulfate reducer most often employed in corrosion studies. Cultures were grown with both lactate and Fe<sup>0</sup> as potential electron donors to replicate the common environmental condition in which organic substrates help fuel the growth of corrosive microbes. Fe<sup>0</sup> was corroded in cultures of a <i>D. vulgaris</i> hydrogenase-deficient mutant with the 1:1 correspondence between Fe<sup>0</sup> loss and H<sub>2</sub> accumulation expected for Fe<sup>0</sup> oxidation coupled to H<sup>+</sup> reduction to H<sub>2</sub>. This result and the extent of sulfate reduction indicated that <i>D. vulgaris</i> was not capable of direct Fe<sup>0</sup>-to-microbe electron transfer even though it was provided with a supplementary energy source in the presence of abundant ferrous sulfide. Corrosion in the hydrogenase-deficient mutant cultures was greater than in sterile controls, demonstrating that H<sub>2</sub> removal was not necessary for the enhanced corrosion observed in the presence of microbes. The parental H<sub>2</sub>-consuming strain corroded more Fe<sup>0</sup> than the mutant strain, which could be attributed to H<sub>2</sub> oxidation coupled to sulfate reduction, producing sulfide that further stimulated Fe<sup>0</sup> oxidation. The results suggest that H<sub>2</sub> consumption is not necessary for microbially enhanced corrosion, but H<sub>2</sub> oxidation can indirectly promote corrosion by increasing sulfide generation from sulfate reduction. The finding that <i>D. vulgaris</i> was incapable of direct electron uptake from Fe<sup>0</sup> reaffirms that direct metal-to-microbe electron transfer has yet to be rigorously described in sulfate-reducing microbes.</p>","PeriodicalId":94145,"journal":{"name":"mLife","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11211667/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141474094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cinnamaldehyde targets SarA to enhance β‐lactam antibiotic activity against methicillin‐resistant Staphylococcus aureus 肉桂醛靶向 SarA,增强β-内酰胺类抗生素对耐甲氧西林金黄色葡萄球菌的活性
Pub Date : 2024-06-14 DOI: 10.1002/mlf2.12121
Jianguo Li, Tingyin Lu, Yue-Fei Chu, Yuejun Zhang, Jing Zhang, Wenzhen Fu, Jian Sun, Yahong Liu, Xiaoping Liao, Yu-Feng Zhou
Methicillin‐resistant Staphylococcus aureus (MRSA) is a current global public health problem due to its increasing resistance to the most recent antibiotic therapies. One critical approach is to develop ways to revitalize existing antibiotics. Here, we show that the phytogenic compound cinnamaldehyde (CIN) and β‐lactam antibiotic combinations can functionally synergize and resensitize clinical MRSA isolates to β‐lactam therapy and inhibit MRSA biofilm formation. Mechanistic studies indicated that the CIN potentiation effect on β‐lactams was primarily the result of inhibition of the mecA expression by targeting the staphylococcal accessory regulator sarA. CIN alone or in combination with β‐lactams decreased sarA gene expression and increased SarA protein phosphorylation that impaired SarA binding to the mecA promoter element and downregulated virulence genes such as those encoding biofilm, α‐hemolysin, and adhesin. Perturbation of SarA–mecA binding thus interfered with PBP2a biosynthesis and this decreased MRSA resistance to β‐lactams. Furthermore, CIN fully restored the anti‐MRSA activities of β‐lactam antibiotics in vivo in murine models of bacteremia and biofilm infections. Together, our results indicated that CIN acts as a β‐lactam adjuvant and can be applied as an alternative therapy to combat multidrug‐resistant MRSA infections.
由于耐甲氧西林金黄色葡萄球菌(MRSA)对最新抗生素疗法的耐药性不断增强,它已成为当前全球的一个公共卫生问题。一种关键的方法是开发出能使现有抗生素重新焕发活力的方法。在这里,我们展示了植物源化合物肉桂醛(CIN)与β-内酰胺类抗生素的组合能在功能上协同增效,使临床 MRSA 分离物对β-内酰胺治疗重新敏感,并抑制 MRSA 生物膜的形成。机理研究表明,CIN 对 β-内酰胺类药物的增效作用主要是通过靶向葡萄球菌附属调节因子 sarA 抑制 mecA 表达的结果。CIN 单独或与β-内酰胺类药物联合使用会降低 sarA 基因的表达,增加 SarA 蛋白的磷酸化,从而影响 SarA 与 mecA 启动子元件的结合,并下调毒力基因,如编码生物膜、α 溶血素和粘附素的基因。因此,扰乱 SarA 与 mecA 的结合会干扰 PBP2a 的生物合成,从而降低 MRSA 对 β 内酰胺类药物的耐药性。此外,在小鼠菌血症和生物膜感染模型中,CIN完全恢复了β-内酰胺类抗生素的抗MRSA活性。总之,我们的研究结果表明,CIN 可作为一种 β-内酰胺类抗生素辅助剂,并可作为一种替代疗法用于抗耐多药 MRSA 感染。
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引用次数: 0
Phage-displayed heptapeptide sequence conjugation significantly improves the specific targeting ability of antimicrobial peptides against Staphylococcus aureus. 噬菌体显示的七肽序列连接显著提高了抗菌肽对金黄色葡萄球菌的特异性靶向能力。
IF 4.5 Q1 MICROBIOLOGY Pub Date : 2024-05-27 eCollection Date: 2024-06-01 DOI: 10.1002/mlf2.12123
Tao Wang, Peng Tan, Qi Tang, Chenlong Zhou, Yakun Ding, Shenrui Xu, Mengda Song, Huiyang Fu, Yucheng Zhang, Xiaohui Zhang, Yueyu Bai, Zhihong Sun, Xi Ma

Broad-spectrum antibacterial drugs often lack specificity, leading to indiscriminate bactericidal activity, which can disrupt the normal microbial balance of the host flora and cause unnecessary cytotoxicity during systemic administration. In this study, we constructed a specifically targeted antimicrobial peptide against Staphylococcus aureus by introducing a phage-displayed peptide onto a broad-spectrum antimicrobial peptide and explored its structure-function relationship through one-factor modification. SFK2 obtained by screening based on the selectivity index and the targeting index showed specific killing ability against S. aureus. Moreover, SFK2 showed excellent biocompatibility in mice and piglet, and demonstrated significant therapeutic efficacy against S. aureus infection. In conclusion, our screening of phage-derived heptapeptides effectively enhances the specific bactericidal ability of the antimicrobial peptides against S. aureus, providing a theoretical basis for developing targeted antimicrobial peptides.

广谱抗菌药物往往缺乏特异性,导致无差别的杀菌活性,从而破坏宿主菌群的正常微生物平衡,并在全身用药时引起不必要的细胞毒性。在这项研究中,我们通过在广谱抗菌肽上引入噬菌体显示肽,构建了一种针对金黄色葡萄球菌的特异性靶向抗菌肽,并通过单因素修饰探索了其结构与功能的关系。根据选择性指数和靶向性指数筛选得到的 SFK2 对金黄色葡萄球菌具有特异性杀灭能力。此外,SFK2 在小鼠和仔猪体内表现出良好的生物相容性,对金黄色葡萄球菌感染有显著疗效。总之,我们对噬菌体衍生七肽的筛选有效地提高了抗菌肽对金黄色葡萄球菌的特异性杀菌能力,为开发靶向抗菌肽提供了理论依据。
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引用次数: 0
Harnessing marine microbiomes to develop a sustainable, all-Atlantic bioeconomy. 利用海洋微生物群发展可持续的全大西洋生物经济。
IF 4.5 Q1 MICROBIOLOGY Pub Date : 2024-05-27 eCollection Date: 2024-06-01 DOI: 10.1002/mlf2.12124
Cristiane Thompson, Alice C Ortmann, Henk Bolhuis, Thulani Makhalanyane, Fabiano Thompson
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引用次数: 0
Microbiome-driven anticancer therapy: A step forward from natural products. 微生物驱动的抗癌疗法:从天然产品向前迈进。
IF 4.5 Q1 MICROBIOLOGY Pub Date : 2024-05-27 eCollection Date: 2024-06-01 DOI: 10.1002/mlf2.12118
Yunxuan Guan, Di Wu, Hui Wang, Ning-Ning Liu

Human microbiomes, considered as a new emerging and enabling cancer hallmark, are increasingly recognized as critical effectors in cancer development and progression. Manipulation of microbiome revitalizing anticancer therapy from natural products shows promise toward improving cancer outcomes. Herein, we summarize our current understanding of the human microbiome-driven molecular mechanisms impacting cancer progression and anticancer therapy. We highlight the potential translational and clinical implications of natural products for cancer prevention and treatment by developing targeted therapeutic strategies as adjuvants for chemotherapy and immunotherapy against tumorigenesis. The challenges and opportunities for future investigations using modulation of the microbiome for cancer treatment are further discussed in this review.

人类微生物组被认为是一种新出现的致癌标志,越来越多的人认识到微生物组是癌症发生和发展的关键影响因素。操纵微生物组,利用天然产品振兴抗癌疗法,有望改善癌症预后。在此,我们总结了我们目前对人类微生物组驱动的影响癌症进展和抗癌疗法的分子机制的理解。我们强调了天然产物通过开发靶向治疗策略作为化疗和免疫疗法的辅助剂来预防和治疗癌症的潜在转化和临床意义。本综述还进一步讨论了未来利用微生物组调节进行癌症治疗研究的挑战和机遇。
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引用次数: 0
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