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Monitoring of mercury ion in environmental media and biological systems using a red emissive fluorescent probe with a large Stokes shift. 利用具有大斯托克斯位移的红色发射型荧光探针监测环境介质和生物系统中的汞离子。
Pub Date : 2024-10-12 DOI: 10.1016/j.saa.2024.125272
Juan Yin, Zejie Wu, Heng Li, Bianli Cao, Wanzhi Wang

The development of practical fluorescent probe for detecting toxic mercury ions (Hg2+) is desirable for environmental assurance and public health. In this study, a new red emissive fluorescent probe (KJL) was designed and synthesized for monitoring trace Hg2+ both in vitro and in vivo with distinct features including ideal response rate (within 4 min), red emission (596 nm), large Stokes shift (162 nm), highly sensitivity (LOD = 4.79 nM) and excellent specificity. KJL also validated the good capability for accurately monitoring trace Hg2+ levels in actual samples (faucet water, drinking water, river water, lake water, urine and serum) and possessed the eye-catching ability in visualization of Hg2+ under environmental/biological conditions, which revealed the great potential of this red-emitting fluorescent probe for practical applications in complex environmental and biological systems.

开发用于检测有毒汞离子(Hg2+)的实用荧光探针是保证环境和公众健康的需要。本研究设计并合成了一种新型红色发射荧光探针(KJL),用于体外和体内监测痕量 Hg2+,该探针具有理想的响应速度(4 分钟内)、红色发射(596 nm)、大斯托克斯位移(162 nm)、高灵敏度(LOD = 4.79 nM)和卓越的特异性等显著特征。KJL 还验证了在实际样品(水龙头水、饮用水、河水、湖水、尿液和血清)中准确监测痕量 Hg2+ 水平的良好能力,以及在环境/生物条件下可视化 Hg2+ 的引人注目的能力,揭示了这种红色发射荧光探针在复杂环境和生物系统中实际应用的巨大潜力。
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引用次数: 0
Mo-doped carbon-dots nanozyme with peroxide-like activity for sensitive and selective smartphone-assisted colorimetric S2- ion detection and antibacterial application. 掺杂钼的碳点纳米酶具有过氧化物样活性,可用于灵敏、选择性的智能手机辅助比色法 S2- 离子检测和抗菌应用。
Pub Date : 2024-10-12 DOI: 10.1016/j.saa.2024.125274
Dai Li, Huajie Chen, Yutao Zheng, Sheng Zhou, Fengyuan Yong, Xiangbo Zhang, Kui Wang, Huiyun Wen, Jiyong Wu, Weiming Xue, Saipeng Huang

Sulfur ion (S2-) plays a significant and considerable role in many living organisms and ecosystems, while its abnormal content can pose a serious hazard to human health and ecological environment. Hence, it is extremely meaningful to construct a highly sensitive and selective analytical platform for S2- detection in complex microenvironment, particularly in biological systems. In this study, phosphomolybdic acid and L-Arg were utilized to prepare a new molybdenum doped carbon-dots nanozyme (Mo-CDs) with great peroxidase-like activity by one-step hydrothermal approach. In the presence of H2O2, Mo-CDs converted 3,3',5,5'-tetramethyl benzidine (TMB) into blue oxTMB, but S2- strongly reduced the blue solution to colorless and then brown, which established significant selectivity toward S2-. Mo-CDs illustrated a wide linear range (2.5 μM-900 μM) and low detection limit (LOD = 76 nM) by ultraviolet and smartphone-assisted visualized colorimetric analysis. Especially, the smartphone-assisted analysis platform successfully realized quick, portable, sensitive and visible identification of S2- with high recovery (95.7-106.7 %) and excellent specificity in water samples. More importantly, Mo-CDs was developed to antibacterial applications based on good peroxidase-like activity. This research not only constructed a new and efficient carbon-dots nanozyme and a low-cost, portable, visual analysis platform for real-time detection of S2-, but also proposed a novel design strategy and methodology for exploiting multifunctional nanozyme detection tool with great practical application.

硫离子(S2-)在许多生物体和生态系统中发挥着重要的作用,而其异常含量会对人类健康和生态环境造成严重危害。因此,构建一个高灵敏度和高选择性的分析平台来检测复杂微环境,尤其是生物系统中的硫离子(S2-)是非常有意义的。本研究利用磷钼酸和 L-Arg 通过一步水热法制备了一种新型的掺钼碳点纳米酶(Mo-CDs),它具有很强的过氧化物酶样活性。在 H2O2 的存在下,Mo-CDs 能将 3,3',5,5'-四甲基联苯胺(TMB)转化为蓝色的 oxTMB,但 S2- 能将蓝色溶液强烈还原为无色,然后变为棕色,这表明 Mo-CDs 对 S2- 具有显著的选择性。通过紫外分析和智能手机辅助可视比色分析,Mo-CDs 具有宽线性范围(2.5 μM-900 μM)和低检测限(LOD = 76 nM)。特别是智能手机辅助分析平台成功实现了对水样中 S2- 的快速、便携、灵敏和可视鉴定,且回收率高(95.7-106.7 %),特异性好。更重要的是,基于良好的过氧化物酶样活性,Mo-CDs 被开发用于抗菌应用。该研究不仅构建了一种新型、高效的碳点纳米酶和一种低成本、便携式、可视化的实时检测 S2-的分析平台,而且提出了一种新的设计策略和方法来开发多功能纳米酶检测工具,具有重要的实际应用价值。
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引用次数: 0
An interesting aggregation induced red shifted emissive and ESIPT active hydroxycoumarin tagged symmetrical azine: Colorimetric and fluorescent turn on-off-on response towards Cu2+ and Cysteine, real sample analysis and logic gate application. 一种有趣的聚集诱导红移发射和 ESIPT 活性羟基香豆素标记对称叠氮:对 Cu2+ 和半胱氨酸的比色和荧光开关响应、实际样品分析和逻辑门应用。
Pub Date : 2024-10-12 DOI: 10.1016/j.saa.2024.125270
Moorthy Mathivanan, Jan Grzegorz Malecki, Balasubramanian Murugesapandian

We report a newly synthesized 7-diethylamino-4-hydroxycoumarin tagged symmetrical azine derivative (SHC), with an interesting color transformation from yellowish green to orange via aggregation induced red shifted emissive (117 nm) feature in THF-H2O mixture. Interestingly, the single crystal X-ray analysis of this molecule demonstrates that two hydroxycoumarin moieties were present in azine unit, among them one of the coumarin units was exist as enol form and another one transferred to keto form via ground state proton transfer reaction. The optical responses of the compound in different solvents exposed the observation of dual emissive bands which corresponds to the presence of ESIPT phenomenon in SHC molecule. Further, this characteristic was confirmed by absorption, emission, solid state structure and time resolved fluorescence decay measurements. Furthermore, the fluorophore, SHC was exploited as a colorimetric and turn on-off-on fluorescent probe for detection of Cu2+ ions and Cysteine (Cys). The 1:1 binding ratio of the probe with Cu2+ and Cys with SHC-Cu2+, was established via Job plot analysis, mass spectral technique and the DFT calculations. The probe, SHC was employed for the detection of copper ions in the environmental real water samples. Finally, the reversible fluorescent turn on-off-on character of the probe, SHC was established to construct the IMPLICATION logic gate application.

我们报告了一种新合成的 7-二乙氨基-4-羟基香豆素标记对称叠氮衍生物(SHC),该衍生物在 THF-H2O 混合物中通过聚集诱导的红移发射(117 纳米)特征实现了从黄绿色到橙色的有趣颜色转变。有趣的是,该分子的单晶 X 射线分析表明,叠氮单元中存在两个羟基香豆素分子,其中一个香豆素单元以烯醇形式存在,另一个则通过基态质子转移反应转变为酮形式。该化合物在不同溶剂中的光学反应显示出双发射带,这表明 SHC 分子中存在 ESIPT 现象。此外,吸收、发射、固态结构和时间分辨荧光衰减测量也证实了这一特征。此外,SHC 荧光团还被用作比色和开关荧光探针,用于检测 Cu2+ 离子和半胱氨酸(Cys)。通过约伯图分析、质谱技术和 DFT 计算,确定了探针与 Cu2+ 和 Cys 与 SHC-Cu2+ 的结合率为 1:1。探针 SHC 被用于检测真实环境水样中的铜离子。最后,利用探针 SHC 的可逆荧光开关特性构建了 IMPLICATION 逻辑门应用。
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引用次数: 0
Rational design of an isatin-based colorimetric and solvatochromic receptor for carbonate ions and its application in molecular-scale logic gates & memory units. 合理设计基于靛红的碳酸根离子比色和溶解变色受体及其在分子级逻辑门和记忆单元中的应用。
Pub Date : 2024-10-12 DOI: 10.1016/j.saa.2024.125273
M M Akhil Kumar, Annasaheb Dhawale, Darshak R Trivedi

A simple and highly sensitive isatin-based colorimetric sensor ISAT 3(a-d) was synthesized through a single-step reaction. The as-prepared receptor ISAT 3b with carbonate ions (CO32- ions) shows a significant red shift in the UV-visible absorption spectra and a visible color change from pale yellow to pink. Also, the receptor ISAT 3b shows unique solvatochromic behavior with CO32- ions in different aprotic solvents and solvent compositions. Moreover, the receptor's pink coloration (absorption maxima at 544 nm) with CO32- ions could be reversible by adding HSO4- ions (attain initial pale-yellow color, absorption maxima at 425 nm), which can be repeatable. The observed color changes with spectral shift and reversibility of the receptor with CO32- ions and HSO4- ions provide "ON-OFF" switching for applying molecular logic gates. Receptors exhibited properties, such as reversibility and repeatability, benefit the design of a molecular-scale sequential memory unit with a display of "Writing-Reading-Erasing-Reading". The real sample analysis was also carried out to prove the practical applicability of receptor (ISAT 3b) for detecting CO32- ions.

通过一步反应合成了一种简单、高灵敏度的异铂基比色传感器 ISAT 3(a-d)。制备的受体 ISAT 3b 与碳酸根离子(CO32- 离子)在紫外-可见吸收光谱中显示出明显的红移,可见光颜色从淡黄色变为粉红色。此外,ISAT 3b 受体与 CO32- 离子在不同的钝化溶剂和溶剂成分中显示出独特的溶解变色行为。此外,加入 HSO4- 离子后,受体与 CO32- 离子的粉红色变色(最大吸收波长为 544 纳米)可以逆转(达到最初的淡黄色,最大吸收波长为 425 纳米),而且可以重复。观察到的颜色变化与光谱移动以及受体与 CO32- 离子和 HSO4- 离子的可逆性为应用分子逻辑门提供了 "ON-OFF "开关。受体所表现出的可逆性和可重复性等特性,有利于设计具有 "写入-读取-递增-读取 "显示的分子级顺序存储器单元。此外,还进行了实际样品分析,以证明受体(ISAT 3b)在检测 CO32- 离子方面的实用性。
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引用次数: 0
Aggregation induced emission "Turn on" ultra-low detection of anti-inflammatory drug flufenamic acid in human urine samples by carbon dots derived from bamboo stem waste. 利用竹茎废料提取的碳点聚合诱导发射 "开启 "超低检测人体尿样中的消炎药氟芬那酸。
Pub Date : 2024-10-11 DOI: 10.1016/j.saa.2024.125278
Subitha Adaikalapandi, T Daniel Thangadurai, S Sivakumar, D Nataraj, Alex Schechter, Nandakumar Kalarikkal, Sabu Thomas

Carbon dot-based fluorescence sensors have attracted research interest for the selective determination of anti-inflammatory drugs in biological fluids and environments. The overdose and accumulation of anti-inflammatory drugs in tissues can cause chronic side effects including abdominal pain, and renal damage. Herein, we report a new fluorescent probe, bamboo stem waste-derived carbon dots (BS-CDs) for highly sensitive detection of Flufenamic acid (FA), a hazardous anti-inflammatory drug. The UV-vis absorption spectra of BS-CDs show a redshifted absorption peak at 283 nm upon the addition of FA suggesting strong binding interaction between BS-CDs and FA molecule. The BS-CDs showed a fluorescence enhancement (∼2-fold) detection for FA (400 μM) in the linear concentration range (0.40 → 0.65 μM) with a limit of detection (LoD; 17 nM) and binding constant (Ka = 1.33 × 10-3 M-1). The time-resolved fluorescence decay analysis showed that the average lifetime of BS-CDs has slightly changed (4.42 → 4.67 ns) by the interaction with FA through the aggregation-induced emission (AIE) process. The interference, pH, and effect of time results suggest that BS-CDs are highly selective probes for FA detection and do not show any interference involvement during FA detection. The confirmation of the structure and morphology changes of BS-CDs after interaction with FA was carried out by XRD, FESEM, HRTEM, FTIR, and Raman spectroscopy. The practicability of the BS-CDs probe was proved by the detection of FA in human urine samples with recovery of 103-109 %. This suggests that the proposed BS-CDs-based 'turn-on' sensor could be used to determine the FA in biological fluids.

基于碳点的荧光传感器在生物液体和环境中选择性测定消炎药方面引起了研究兴趣。过量服用消炎药并在组织中蓄积会导致慢性副作用,包括腹痛和肾损伤。在此,我们报告了一种新型荧光探针--竹茎废弃物衍生碳点(BS-CDs),用于高灵敏度检测有害消炎药氟芬那酸(FA)。加入氟芬那酸后,BS-CDs 的紫外-可见吸收光谱在 283 纳米处出现红移吸收峰,表明 BS-CDs 与氟芬那酸分子之间有很强的结合作用。在线性浓度范围(0.40 → 0.65 μM)内,BS-CDs 对 FA(400 μM)的检测荧光增强(∼2 倍),检测限(LoD;17 nM)和结合常数(Ka = 1.33 × 10-3 M-1)。时间分辨荧光衰减分析表明,通过聚集诱导发射(AIE)过程与 FA 相互作用,BS-CD 的平均寿命略有改变(4.42 → 4.67 ns)。干扰、pH 值和时间的影响结果表明,BS-CDs 是一种高选择性的 FA 检测探针,在 FA 检测过程中不会受到任何干扰。XRD、FESEM、HRTEM、傅立叶变换红外光谱和拉曼光谱证实了 BS-CD 与 FA 作用后的结构和形态变化。BS-CDs 探针在人体尿液样本中检测到了 FA,回收率为 103-109%,这证明了 BS-CDs 探针的实用性。这表明所提出的基于 BS-CDs 的 "开启式 "传感器可用于测定生物液体中的 FA。
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引用次数: 0
A portable optical sensor combining smartphone with phycocyanin-based fluorescent test paper for rapid, visual and on-site detection of CO32. 一种便携式光学传感器,结合了智能手机和基于藻青素的荧光试纸,用于快速、可视和现场检测 CO32。
Pub Date : 2024-10-11 DOI: 10.1016/j.saa.2024.125276
Yifeng Xu, Rui Sun, Shubo Wang, Fei Fei, Zhangyu Gan, Pin Zhou

With the development of global industry, carbon dioxide emissions surged. The conversion of carbon dioxide from the air results in some CO32-, which can exacerbate environmental disasters like ocean acidification. Therefore, the content of CO32- in seawater is an important indicator of the degree of ocean acidification. In this study, natural fluorescent protein phycocyanin (PC) was used as a fluorescent probe, and a fluorescence detection method was established for quantitative monitoring of CO32- with quick response time (within 50 s), high sensitivity, and selectivity. The fluorescence quenching phenomenon between PC and CO32- was mainly attributed to static quenching. The limit of detection (LOD) was 0.42 μM and the method was successfully applied to monitor CO32- in tap water and seawater, acquiring satisfactory recovery between 99.28 % and 106.40 %. More importantly, paper-based test strips were easily fabricated using PC, enabling the rapid, visual, and on-site detection of CO32- with the aid of a smartphone. The visual detection integrated with the smartphone was converted to data information (RGB value) through a Color Picker APP and successfully used for quantitative identification of CO32-. By capturing fluorescent images and analyzing the corresponding RGB value via a smartphone, the linear calibration ranged from 0.5 μM to 500.0 μM with LOD of 0.11 μM was obtained. Satisfactory recoveries were acquired in tap water (98.00 %-107.50 %) and seawater (97.30 %-101.74 %), respectively. Therefore, integrating the PC fluorescent paper with a smartphone realizes the rapid, visual, and on-site detection of CO32- in the water environment, which is expected to broaden application prospects of monitoring ocean acidification degree.

随着全球工业的发展,二氧化碳排放量激增。空气中的二氧化碳在转化过程中会产生一些 CO32-,这会加剧海洋酸化等环境灾害。因此,海水中 CO32- 的含量是衡量海洋酸化程度的重要指标。本研究以天然荧光蛋白藻蓝蛋白(PC)为荧光探针,建立了一种响应时间快(50 秒内)、灵敏度高、选择性强的荧光检测方法,用于定量监测 CO32-。PC 与 CO32- 之间的荧光淬灭现象主要归因于静态淬灭。该方法的检出限(LOD)为 0.42 μM,并成功用于监测自来水和海水中的 CO32-,回收率在 99.28 % 至 106.40 % 之间。更重要的是,该方法利用个人电脑轻松制作了纸质试纸,可借助智能手机快速、直观地现场检测 CO32-。与智能手机集成的视觉检测通过 Color Picker APP 转换为数据信息(RGB 值),并成功用于 CO32- 的定量鉴定。通过智能手机捕捉荧光图像并分析相应的 RGB 值,获得了从 0.5 μM 到 500.0 μM 的线性定标范围,LOD 为 0.11 μM。在自来水(98.00 %-107.50 %)和海水(97.30 %-101.74 %)中分别获得了令人满意的回收率。因此,将 PC 荧光纸与智能手机相结合,可实现水环境中 CO32- 的快速、直观和现场检测,有望拓宽海洋酸化程度监测的应用前景。
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引用次数: 0
Isomerization enhanced fluorescence brightness of benzobisthiadiazole-based NIR-II fluorophores for highly efficient fluorescence imaging: A theoretical perspective. 用于高效荧光成像的基于苯并二噻二唑的 NIR-II 荧光团的异构化增强荧光亮度:理论视角。
Pub Date : 2024-10-11 DOI: 10.1016/j.saa.2024.125282
Yuying Du, Yujin Zhang, Yulong Xu, Meina Zhang, Jiancai Leng, Wei Hu

As a cutting-edge technique, fluorescence imaging in the second near-infrared window (NIR-II) is vital for both biomedical research and clinical applications. However, its intravital imaging capacity has been restricted by the extremely limited brightness of NIR-II fluorophores. To address this challenge, we elucidated the inner mechanism of constructing high-performance NIR-II chromophores based on molecular isomer engineering from detailed computational investigations. Herein, three pairs of cis-trans isomers (cis-1, 2, 3 and trans-1, 2, 3) are designed by attaching amino, methoxyl and nitro moieties to different positions on the donor-acceptor-donor molecular skeleton with benzobisthiadiazole as the acceptor and triphenylamine as the donor. All the compounds feature efficient NIR-II emission ranging in 1000-1164 nm, and the photophysical characterizations are regulated by molecular isomer manipulation. Interestingly, fluorescence quantum yields of cis-isomers are higher than those of their trans-counterparts. These enhancements can be attributed to the significant reduction in non-radiative transition, as evidenced by the non-adiabatic excitation energy, non-adiabatic electron coupling and electron-vibration coupling. Meanwhile, fluorophores with nitro terminal group exhibit superior performance facilitated by the prominently intramolecular charge transfer. As a result, cis-3 achieves an optimal brightness maxima of 196.36 M-1 cm-1 at 632 nm. Notably, the energy gap and the hole-electron related H index are respectively identified as strongly relevant to the emission wavelength and brightness, making them capable of evaluating the feasibility of fluorophores as effective NIR-II candidates. These findings highlight the correlations between molecular geometry and luminescent properties, which will inspire more insights into the development of highly efficient NIR-II fluorophores through rational isomer engineering for biomedical applications.

作为一项前沿技术,第二近红外窗口(NIR-II)荧光成像对于生物医学研究和临床应用都至关重要。然而,由于 NIR-II 荧光团的亮度极其有限,其体内成像能力一直受到限制。为了应对这一挑战,我们通过详细的计算研究,阐明了基于分子异构体工程构建高性能 NIR-II 发色团的内在机理。在这里,我们以苯并二噻二唑为受体,三苯胺为供体,通过在供体-受体-受体分子骨架的不同位置上连接氨基、甲氧基和硝基,设计出了三对顺反异构体(顺-1, 2, 3 和反-1, 2, 3)。所有化合物都具有 1000-1164 纳米范围内的高效近红外-II 发射,其光物理特性受分子异构体操作的调节。有趣的是,顺式异构体的荧光量子产率高于反式异构体。这些提高可归因于非辐射转变的显著减少,非绝热激发能量、非绝热电子耦合和电子振动耦合都证明了这一点。同时,带有硝基末端基团的荧光团由于分子内电荷转移显著而表现出更优越的性能。因此,顺式-3 在 632 纳米波长处达到了 196.36 M-1 cm-1 的最佳亮度最大值。值得注意的是,能隙和与空穴电子相关的 H 指数分别被确定为与发射波长和亮度密切相关,因此它们能够评估荧光团作为有效的近红外-II 候选物的可行性。这些发现凸显了分子几何形状与发光特性之间的相关性,这将启发人们更深入地了解如何通过合理的异构体工程开发高效的近红外-II荧光团,以应用于生物医学领域。
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引用次数: 0
A novel controllable nanocyclic plasma coupled array in SERS trace detection of multi-component pollutants. 用于 SERS 多组分污染物痕量检测的新型可控纳米环等离子体耦合阵列。
Pub Date : 2024-10-11 DOI: 10.1016/j.saa.2024.125271
Xuanyang Ding, Zhuangzhuang Ling, Jinghuai Fang, Mingfei Cheng, Jing Wu

The development and design of a novel, uniform and highly active local electromagnetic field enhanced structure is crucial for expanding Surface-enhanced Raman Scattering (SERS) applications. In this study, we developed Ag ring-coupled nanoarrays (Ag RCNAs) with controllable nanogaps using a substrate rotary evaporation coating technique with self-assembled polystyrene (PS) microspheres as templates. This straightforward and cost-effective method efficiently prepares plasma-coupled nanoarrays. Ag RCNAs demonstrated high sensitivity in detecting organic dyes, our prepared Ag RCNAs showed high sensitivity (with the limit of detection of 10-8 M), high signal reproducibility (with the relative standard deviation of 6.73 %). Furthermore, Ag RCNAs showed remarkable sensitivity to a broad spectrum of dyes in river water, indicating the large-area uniform and highly active circular-ring-shaped nanogaps can realize highly sensitive detection of various pollutants. This approach offers advantages in electromagnetic field enhancement, tunable nanogaps, uniformity, reproducibility, and recyclability, making it promising for applications in environmental monitoring, bioassays, food safety, and medical diagnostics.

开发和设计一种新颖、均匀和高活性的局部电磁场增强结构对于扩大表面增强拉曼散射(SERS)的应用至关重要。在本研究中,我们以自组装聚苯乙烯(PS)微球为模板,采用基底旋转蒸发镀膜技术开发了具有可控纳米间隙的银环耦合纳米阵列(Ag RCNAs)。这种简单易行、经济高效的方法能有效制备等离子体耦合纳米阵列。我们制备的 Ag RCNAs 在检测有机染料方面表现出高灵敏度(检测限为 10-8 M)、高信号重现性(相对标准偏差为 6.73 %)。此外,银 RCNAs 对河水中的多种染料都表现出显著的灵敏度,这表明大面积均匀、高活性的环形纳米缝隙可以实现对多种污染物的高灵敏度检测。这种方法具有电磁场增强、可调纳米间隙、均匀性、可重复性和可回收性等优点,因此在环境监测、生物测定、食品安全和医疗诊断等领域具有广阔的应用前景。
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引用次数: 0
Raman spectroscopic quantification of polyethylene particles in water using polydimethylsiloxane-coated nickel foam as a particle-capturing platform. 利用聚二甲基硅氧烷涂层泡沫镍作为颗粒捕获平台,对水中的聚乙烯颗粒进行拉曼光谱定量。
Pub Date : 2024-10-10 DOI: 10.1016/j.saa.2024.125269
Sanghoon Cho, Sangjae Kim, Yunjung Kim, Hoeil Chung

Nickel foam (NF) was evaluated as a medium for the capture of polyethylene (PE) particles in water. NF is a hydrophobic and porous material with a large surface area, making it a promising candidate for attracting PE particles. However, the particle-capturing efficiency using bare NF was only 69.5%. To increase capturing efficiency, a circular polydimethylsiloxane (PDMS)-coated NF (PDMS@NF, diameter: 6 mm) was employed to enhance the hydrophobicity. The capturing efficiency using the PDMS@NF was substantially increased to 97.6 % owing to the increase in hydrophobicity. To quantify the captured PE particles on/in the PDMS@NF using Raman spectroscopy, a wide area illumination (WAI) scheme providing 6 mm-diameter laser illumination was adopted to fully cover the PDMS@NF for representative spectroscopic sampling and accurate quantification. The intensity ratios of PE to PDMS peaks in the collected spectra clearly increased with the quantity of dispersed PE particles (0.1 ∼ 4.0 mg range, R2: 0.992) in the water samples, and the limit of detection was 0.08 mg. Moreover, the capturing efficiencies for polypropylene (PP), polystyrene (PS), and polyethylene terephthalate (PET) particles (1 mg of each) using the PDMS@NF were also superior, ranging from 96.4 to 98.2 %. Therefore, the proposed scheme incorporating the PDMS@NF as a particle-capturing and Raman measurement platform has potential as a method for on-line detection of microplastics in water.

对泡沫镍(NF)作为捕获水中聚乙烯(PE)颗粒的介质进行了评估。NF 是一种疏水性多孔材料,具有较大的表面积,因此很有希望吸引聚乙烯颗粒。然而,使用裸 NF 的颗粒捕获效率仅为 69.5%。为了提高捕获效率,采用了圆形聚二甲基硅氧烷(PDMS)涂层 NF(PDMS@NF,直径:6 毫米)来增强疏水性。由于疏水性的增加,使用 PDMS@NF 的捕获效率大幅提高到 97.6%。为了利用拉曼光谱对 PDMS@NF 上/中捕获的聚乙烯颗粒进行量化,采用了广域照明(WAI)方案,提供直径为 6 毫米的激光照射,以完全覆盖 PDMS@NF,从而获得具有代表性的光谱取样和精确量化。采集到的光谱中 PE 与 PDMS 峰的强度比随着水样中分散的 PE 粒子数量(0.1 ∼ 4.0 mg 范围,R2:0.992)的增加而明显增加,检测限为 0.08 mg。此外,PDMS@NF 对聚丙烯(PP)、聚苯乙烯(PS)和聚对苯二甲酸乙二醇酯(PET)颗粒(各 1 毫克)的捕获效率也很高,在 96.4% 到 98.2% 之间。因此,将 PDMS@NF 作为颗粒捕获和拉曼测量平台的拟议方案具有在线检测水中微塑料的潜力。
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引用次数: 0
A colorimetric fluorescent probe for reversible detection of HSO3-/H2O2 and effective discrimination of HSO3-/ClO- and its application in food and bioimaging. 用于可逆检测 HSO3-/H2O2 和有效区分 HSO3-/ClO- 的比色荧光探针及其在食品和生物成像中的应用。
Pub Date : 2024-10-10 DOI: 10.1016/j.saa.2024.125275
Yuexing Gao, Yan Peng, Lei Shi, Siyun Zhang, Ruiyang Bai, Yunhe Lang, Yonggui He, Buyue Zhang, Ziyi Zhang, Xiufeng Zhang

In view of the significant role of reactive sulfur species (RSS) and reactive oxygen species (ROS) in maintaining the redox homeostasis of organisms, we proposed a colorimetric fluorescent probe (HTN) for reversible detection of HSO3-/H2O2 and effective discrimination of HSO3-/ClO-. C = C is the active site for the Michael addition of HSO3- and the oxidation of ClO-. When HTN interacts with HSO3- and ClO-, it exhibits fluorescence quenching. The addition of oxidizing H2O2 to the system can restore the conjugate structure of the addition product of HSO3- (HTN-HSO3-) and the fluorescence recovery, but it cannot restore the structure of the oxidation product of ClO- (HTN-ClO-). By studying the change of the reversibility/non-reversibility of the probe structure with the addition of H2O2, the purpose of reversible detection of HSO3-/H2O2 and distinguishing HSO3-/ClO- is achieved. In addition, HTN can not only be used as a fluorescent ink to detect HSO3- on the test paper, but also has excellent detection effect on HSO3- and ClO- in real food samples and water samples. Meantime, HTN has good biocompatibility and can target mitochondria to achieve reversible detection of HSO3-/H2O2 and effective discrimination of HSO3-/ClO- in living cells. Therefore, HTN has great potential as a molecular tool for studying redox homeostasis in the interaction network of complex living systems.

鉴于活性硫物种(RSS)和活性氧物种(ROS)在维持生物体氧化还原平衡中的重要作用,我们提出了一种比色荧光探针(HTN),用于可逆检测 HSO3-/H2O2,并有效区分 HSO3-/ClO-。C = C 是 HSO3- 迈克尔加成和 ClO- 氧化的活性位点。当 HTN 与 HSO3- 和 ClO- 作用时,会出现荧光淬灭。向体系中加入氧化性 H2O2 可以恢复 HSO3- 的加成产物(HTN-HSO3-)的共轭结构和荧光恢复,但不能恢复 ClO- 的氧化产物(HTN-ClO-)的结构。通过研究加入 H2O2 后探针结构的可逆/不可逆变化,达到了可逆检测 HSO3-/H2O2、区分 HSO3-/ClO-的目的。此外,HTN 不仅可以作为荧光墨水检测试纸上的 HSO3-,而且对实际食品样品和水样中的 HSO3- 和 ClO- 也有很好的检测效果。同时,HTN 具有良好的生物相容性,可靶向线粒体实现对 HSO3-/H2O2 的可逆检测,并能有效区分活细胞中的 HSO3-/ClO-。因此,HTN 作为一种分子工具,在研究复杂生命系统相互作用网络中的氧化还原稳态方面具有巨大潜力。
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引用次数: 0
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Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
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