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Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy最新文献

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UV/visible absorption maxima prediction of water-soluble organic compounds and generation of library of new organic compounds. 预测水溶性有机化合物的紫外/可见吸收最大值并生成新的有机化合物库。
Pub Date : 2024-11-19 DOI: 10.1016/j.saa.2024.125453
Aftab Farrukh, Ibrahim A Shaaban, Mohammed A Assiri, Mudassir Hussain Tahir, Zeinhom M El-Bahy

In this study, UV/visible absorption maxima of organic compounds are predicted with the help of machine learning (ML). Four ML models are evaluated, the gradient boosting model has performed best. We also analyzed feature importance. Using Python-based tools, we generated and visualized a new set of 5,000 organic compounds. These compounds were screened based on their predicted UV/visible absorption maxima, selecting those with red-shifted absorption. The assessment of synthetic accessibility indicated that most of the chosen compounds are relatively easy to synthesize.

在这项研究中,利用机器学习(ML)预测了有机化合物的紫外/可见吸收最大值。我们评估了四种 ML 模型,其中梯度提升模型表现最佳。我们还分析了特征的重要性。利用基于 Python 的工具,我们生成并可视化了一组新的 5,000 种有机化合物。我们根据预测的紫外/可见吸收最大值对这些化合物进行了筛选,选出了具有红移吸收的化合物。对合成可得性的评估表明,大多数被选中的化合物都比较容易合成。
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引用次数: 0
Fluorescent probes with dual-targeting organelles monitor polarity in non-alcoholic fatty liver disease. 具有双靶向细胞器的荧光探针可监测非酒精性脂肪肝的极性。
Pub Date : 2024-11-19 DOI: 10.1016/j.saa.2024.125455
Yi Deng, Jie Wang, Sichen Zhang, Jiale Li, Aobo Sun, Xue Zhang, Lei Hu, Chunfei Wang, Hui Wang

Non-alcoholic fatty liver disease (NAFLD) becomes a world health issue due to its rising prevalence and lack of a definitive pathogeny. However, the excessive accumulation of fat droplets has been recognized as a crucial characteristic of NAFLD, accompanied with endoplasmic reticulum stress in its onset and progression as well. Therefore, real-time monitoring the dynamic of lipid droplets (LDs) and endoplasmic reticulum (ER) within cells is paramount. In this regard, four D-A-π-D structural fluorescent probes COB1-COB4 were designed and synthesized wherein coumarin connected with carbazole acted as precursors while the side chains attached to carbazole groups are different. Here, probes COB1-COB4 exhibited high sensitivity towards polarity, while COB2 was chosen for further study attributing to its excellent anti-interference property. Cell imaging demonstrated that COB2 could accurately target both LDs and ER at the same time and monitor the changes of the two organelles under different physiological conditions. Notably, probe COB2 also exhibited the ability to distinguish normal liver from fatty liver at the tissue level. The above results lay an experimental foundation for developing novel dual-targeted probes with potential for early diagnosis of non-alcoholic fatty liver.

非酒精性脂肪肝(NAFLD)因其发病率不断上升和缺乏明确的病原体而成为一个世界性的健康问题。然而,脂肪滴的过度积聚已被认为是非酒精性脂肪肝的一个重要特征,在其发生和发展过程中还伴有内质网应激。因此,实时监测细胞内脂滴(LDs)和内质网(ER)的动态至关重要。为此,我们设计并合成了四种 D-A-π-D 结构荧光探针 COB1-COB4,其中香豆素与咔唑相连作为前体,而连接到咔唑基团的侧链则各不相同。其中,探针 COB1-COB4 对极性具有高灵敏度,而 COB2 则因其出色的抗干扰性能而被选作进一步研究的对象。细胞成像结果表明,COB2 可以同时准确地靶向 LD 和 ER,并监测这两种细胞器在不同生理条件下的变化。值得注意的是,探针 COB2 还能在组织水平上区分正常肝脏和脂肪肝。上述结果为开发新型双靶向探针奠定了实验基础,有望用于非酒精性脂肪肝的早期诊断。
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引用次数: 0
Rapid detection of thiram on apple surfaces using a flexible and sticky SERS substrate coupled with chemometric methods. 利用柔性和粘性 SERS 基底以及化学计量学方法快速检测苹果表面的噻喃。
Pub Date : 2024-11-19 DOI: 10.1016/j.saa.2024.125435
Sasa Peng, Zhilong Zhang, Jialin Guo, Tianchen Ma, Dongli Liu

In this paper, we developed a simple, rapid and sensitive method for detection of thiram on apple surfaces by surface enhance Raman spectroscopy (SERS) combined with chemometric methods. Ag NCs (Ag nanocubes) were firstly prepared by a sulfide-mediated polyol method. Then the flexible and adhesive Ag NCs@PDMS substrates were obtained by combining Ag NCs self-assembled films with PDMS films. Thiram residues on apple surfaces were transferred to the substrate using adhesion properties of Ag NCs@PDMS. And the SERS spectra were obtained by Raman microscopy and analyzed with chemometric methods. The results were analyzed by principal component analysis (PCA), for the limit of detection (LOD) of thriam on apple surfaces was 0.01 ppm. Principal component regression (PCR) and partial least squares regression (PLSR) were explored to develop quantitative models. Both models represented higher correlation coefficients (close to 1), but PLSR models exhibited better predictive performance, with the correlation coefficient was 0.99282 with a low root mean squared error of calibration (RMSEC = 0.438) and root mean squared error of validation (RMSECV = 0.597). The developed SERS method based on Ag NCs@PDMS substrate provide a simpler and more sensitive way to monitor thiram on apple surfaces.

本文采用表面增强拉曼光谱(SERS)结合化学计量学方法,开发了一种简单、快速、灵敏的苹果表面噻拉姆检测方法。首先用硫化物介导的多元醇法制备了 Ag NCs(Ag 纳米立方体)。然后将 Ag NCs 自组装薄膜与 PDMS 薄膜结合,获得了柔性和粘性 Ag NCs@PDMS 基底。利用 Ag NCs@PDMS 的粘附特性,将苹果表面的噻拉姆残留物转移到基底上。通过拉曼显微镜获得了 SERS 光谱,并用化学计量学方法进行了分析。通过主成分分析法(PCA)对结果进行了分析,苹果表面的噻吩检测限(LOD)为 0.01 ppm。探索了主成分回归(PCR)和偏最小二乘回归(PLSR)来建立定量模型。两种模型的相关系数都较高(接近 1),但 PLSR 模型的预测性能更好,相关系数为 0.99282,校准的均方根误差(RMSEC = 0.438)和验证的均方根误差(RMSECV = 0.597)都较低。所开发的基于 Ag NCs@PDMS 基质的 SERS 方法为监测苹果表面的噻喃提供了一种更简单、更灵敏的方法。
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引用次数: 0
A turn-on AIE dual-channel fluorescent probe for sensing Cr3+/ClO- and application in cell imaging. 用于感测 Cr3+/ClO- 的开启式 AIE 双通道荧光探针及其在细胞成像中的应用。
Pub Date : 2024-11-17 DOI: 10.1016/j.saa.2024.125444
Huizhen Wang, Yan Tang, Kezhen Gou, Dingxin Qiu, Cheng Du, Zhengfeng Xie, Guanjun Chang

A Cr3+/ClO--enhanced fluorescent probe, DNS (5-(dimethylamino)-N'-(2-hydroxy-4,6-dimethoxybenzylidene)-naphthalene-1-sulfonyl hydrazide), with aggregation-induced emission (AIE) properties was synthesized using dansylhydrazide and 4,6-dimethoxysalicylaldehyde as starting materials. The probe rapidly and selectively detects Cr3+ and ClO- in a solvent system of H2O/DMSO (2:8). Upon binding with Cr3+/ClO-, the probe exhibits a significant fluorescence enhancement, with minimal interference from other ions. The detection limits (LOD) were determined to be 5.36 × 10-7 mol/L for Cr3+ and 3.65 × 10-7 mol/L for ClO-. The binding mechanisms of DNS with Cr3+/ClO- were investigated through Job's plot, 1H NMR titration, and mass spectrometry. Furthermore, the probe's low cytotoxicity and biocompatibility suggest its potential for detecting exogenous Cr3+/ClO- and endogenous ClO- in living cells. DNS shows promise for real-time detection and bioimaging applications.

以丹酰肼和 4,6-二甲氧基水杨醛为起始原料,合成了一种具有聚集诱导发射(AIE)特性的 Cr3+/ClO- 增强荧光探针 DNS(5-(dimethylamino)-N'-(2-hydroxy-4,6-dimethoxybenzylidene)-naphthalene-1-sulfonyl hydrazide)。该探针可在 H2O/DMSO (2:8) 溶剂体系中快速、选择性地检测 Cr3+ 和 ClO-。与 Cr3+/ClO- 结合后,探针的荧光明显增强,其他离子的干扰极小。经测定,Cr3+ 的检测限(LOD)为 5.36 × 10-7 mol/L,ClO- 的检测限(LOD)为 3.65 × 10-7 mol/L。通过约伯图、1H NMR 滴定和质谱分析,研究了 DNS 与 Cr3+/ClO- 的结合机制。此外,该探针的低细胞毒性和生物相容性表明,它具有在活细胞中检测外源 Cr3+/ClO- 和内源 ClO- 的潜力。DNS 显示了实时检测和生物成像应用的前景。
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引用次数: 0
A graphene quantum dots based dual-modal fluorometric and visualized detection of copper ions. 基于石墨烯量子点的铜离子荧光和可视化双模式检测。
Pub Date : 2024-11-17 DOI: 10.1016/j.saa.2024.125442
Yuping Guo, Qihan Huang, Fengzhou Xu, Zhishan Luo, Yaojin Wei, Zhijun Chen, Zhichao Zeng, Huihui Zhang, Hui Shi

In this study, we present a dual-modal fluorometric and visualized detection method for Cu2+ ion, leveraging the synergistic properties of graphene quantum dots (GQDs) and Cu2+ ion catalyzed Fenton-like reaction. The Fenton-like reaction of Cu2+ ions and ascorbate generates highly reactive hydroxyl radical (·OH), which effectively disrupt the structure of GQDs, leading to fluorescence quenching. Under optimized conditions, the fluorescence quenching degree exhibited a linear correlation with Cu2+ concentration within the range of 40 to 2000 nM, enabling the detection of Cu2+ ions as low as 40 nM. Furthermore, we demonstrated the feasibility of semi-quantitative visual detection of Cu2+ ion concentrations in water using a portable ultraviolet instrument. The method achieved a minimum detectable concentration of Cu2+ ion as low as 10 μM, surpassing the maximum contaminant level goals of 20.47 μM set by the EPA and the guideline value of 31.47 μM recommended by the WHO. As such, this approach holds promise as a point-of-care testing (POCT) method for Cu2+ ion detection during copper pollution emergency events in water. Additionally, this method can be adapted for the detection of ascorbic acid. Our findings showcase the potential of this dual-modal detection approach, offering a sensitive, rapid, and efficient means for detecting Cu2+ ion, thereby contributing to environmental monitoring and public health applications.

在本研究中,我们利用石墨烯量子点(GQDs)和 Cu2+ 离子催化的 Fenton 类反应的协同特性,提出了一种 Cu2+ 离子的荧光和可视化双模式检测方法。Cu2+ 离子和抗坏血酸的 Fenton 类反应会产生高活性羟基自由基(-OH),从而有效破坏 GQDs 的结构,导致荧光淬灭。在优化条件下,荧光淬灭度在 40 到 2000 nM 范围内与 Cu2+ 浓度呈线性相关,从而实现了对低至 40 nM 的 Cu2+ 离子的检测。此外,我们还证明了利用便携式紫外仪器对水中 Cu2+ 离子浓度进行半定量视觉检测的可行性。该方法实现了低至 10 μM 的 Cu2+ 离子最低可检测浓度,超过了美国环保局设定的 20.47 μM 的最大污染物水平目标和世界卫生组织推荐的 31.47 μM 的指导值。因此,这种方法有望成为在铜污染紧急事件期间检测水中 Cu2+ 离子的护理点检测 (POCT) 方法。此外,这种方法还可用于检测抗坏血酸。我们的研究结果展示了这种双模式检测方法的潜力,它提供了一种灵敏、快速、高效的 Cu2+ 离子检测方法,从而有助于环境监测和公共卫生应用。
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引用次数: 0
Colorimetric fluorescence of the 1,10-phenantholineyl-imidazole sensor probe for the selective detection of Zn2+ and Cd2+ ions. 用于选择性检测 Zn2+ 和 Cd2+ 离子的 1,10-菲啶基咪唑传感器探针的比色荧光。
Pub Date : 2024-11-17 DOI: 10.1016/j.saa.2024.125436
Chang Liu, Lu Ren, Xiudong Yang, Yanqi Liu, Huihong Zhang, Wanru Jia, Dawei Zhang

A colorimetric fluorescent probe, 4-(1H-imidazolo[4,5-f][1,10]phenanthroline-2-yl)-N, N-diphenylaniline (PIN), was designed, synthesized and characterized for the sensitive and selective detection of Zn2+ and Cd2+. The color of the solution changed from blue to yellow visible to the naked eye with the addition of Zn2+ and Cd2+. The probe PIN showed good anti-interference to Zn2+ and Cd2+ in the presence of a variety of metal ions, and the fluorescence intensity showed a good linear relationship with the concentrations of Zn2+ and Cd2+, with detection limits of 34.84 nM and 35.76 nM, respectively. The probe PIN complexed 2:1 with Zn2+ and Cd2+, and the complexation constants were 1.03 × 104 M-1 (PIN - Zn2+, R2 = 0.9971) and 1.50 × 104 M-1 (PIN - Cd2+, R2 = 0.9981), respectively. In addition, the PIN could be recovered by EDTA and could be effectively monitored for Zn2+ and Cd2+ at pH 4-11, with good results in actual water samples. The HepG-2 cells maintained over 95 % of viability after 24 h exposure to PIN, which identified the extremely low toxic of PIN and could be used for in vivo cell imaging.

本研究设计、合成并鉴定了一种比色荧光探针--4-(1H-咪唑并[4,5-f][1,10]菲罗啉-2-基)-N, N-二苯基苯胺(PIN),用于灵敏、选择性地检测 Zn2+ 和 Cd2+。加入 Zn2+ 和 Cd2+ 后,溶液的颜色由蓝色变为肉眼可见的黄色。在多种金属离子存在的情况下,探针 PIN 对 Zn2+ 和 Cd2+ 具有良好的抗干扰性,荧光强度与 Zn2+ 和 Cd2+ 的浓度呈良好的线性关系,检测限分别为 34.84 nM 和 35.76 nM。探针 PIN 与 Zn2+ 和 Cd2+ 的络合比例为 2:1,络合常数分别为 1.03 × 104 M-1 (PIN - Zn2+,R2 = 0.9971)和 1.50 × 104 M-1 (PIN - Cd2+,R2 = 0.9981)。此外,PIN 可通过 EDTA 回收,并可在 pH 值为 4-11 时有效监测 Zn2+ 和 Cd2+,在实际水样中效果良好。HepG-2 细胞在接触 PIN 24 小时后仍能保持 95% 以上的活力,这表明 PIN 的毒性极低,可用于体内细胞成像。
{"title":"Colorimetric fluorescence of the 1,10-phenantholineyl-imidazole sensor probe for the selective detection of Zn<sup>2+</sup> and Cd<sup>2+</sup> ions.","authors":"Chang Liu, Lu Ren, Xiudong Yang, Yanqi Liu, Huihong Zhang, Wanru Jia, Dawei Zhang","doi":"10.1016/j.saa.2024.125436","DOIUrl":"https://doi.org/10.1016/j.saa.2024.125436","url":null,"abstract":"<p><p>A colorimetric fluorescent probe, 4-(1H-imidazolo[4,5-f][1,10]phenanthroline-2-yl)-N, N-diphenylaniline (PIN), was designed, synthesized and characterized for the sensitive and selective detection of Zn<sup>2+</sup> and Cd<sup>2+</sup>. The color of the solution changed from blue to yellow visible to the naked eye with the addition of Zn<sup>2+</sup> and Cd<sup>2+</sup>. The probe PIN showed good anti-interference to Zn<sup>2+</sup> and Cd<sup>2+</sup> in the presence of a variety of metal ions, and the fluorescence intensity showed a good linear relationship with the concentrations of Zn<sup>2+</sup> and Cd<sup>2+</sup>, with detection limits of 34.84 nM and 35.76 nM, respectively. The probe PIN complexed 2:1 with Zn<sup>2+</sup> and Cd<sup>2+</sup>, and the complexation constants were 1.03 × 10<sup>4</sup> M<sup>-1</sup> (PIN - Zn<sup>2+</sup>, R<sup>2</sup> = 0.9971) and 1.50 × 10<sup>4</sup> M<sup>-1</sup> (PIN - Cd<sup>2+</sup>, R<sup>2</sup> = 0.9981), respectively. In addition, the PIN could be recovered by EDTA and could be effectively monitored for Zn<sup>2+</sup> and Cd<sup>2+</sup> at pH 4-11, with good results in actual water samples. The HepG-2 cells maintained over 95 % of viability after 24 h exposure to PIN, which identified the extremely low toxic of PIN and could be used for in vivo cell imaging.</p>","PeriodicalId":94213,"journal":{"name":"Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy","volume":"328 ","pages":"125436"},"PeriodicalIF":0.0,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142690185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Application of excitation-emission matrix fluorescence spectroscopy and chemometrics for quantitative analysis of emulsified oil concentration. 将激发-发射矩阵荧光光谱和化学计量学应用于乳化油浓度的定量分析。
Pub Date : 2024-11-17 DOI: 10.1016/j.saa.2024.125423
Zhiwei Wang, Peiliang Wu, Yuhan Zhao, Xinyi Li, Deming Kong

Emulsified oil concentration is an important index for quantitative analysis of sea surface oil spill pollution, and the development of a fast and effective quantitative analysis method for emulsified oil concentration plays a crucial role in the estimation of oil spill volume and post-spill assessment. A quantitative analysis method for emulsified oil concentration based on excitation-emission matrix (EEM) fluorescence spectroscopy and chemometrics was proposed. Firstly, the EEM fluorescence spectra of two emulsified oils were measured using a FLS1000 fluorescence spectrometer. Then, the measured EEM fluorescence spectra were decomposed by parallel factor analysis (PARAFAC), and several key excitation wavelengths were filtered from the loading matrix obtained from the decomposition. Subsequently, the three-band fluorescence index (TBFI) at these excitation wavelengths was calculated and combined with the optimal band selection algorithm, from which the optimal emission band combinations were selected. Finally, the selected optimal emission bands were combined with partial least squares regression (PLSR) to establish a prediction model for emulsified oil concentration. By comparing the prediction results with those based on PARAFAC-PLSR and multivariate curve resolved-alternating least squares (MCR-ALS)-PLSR models, the TBFI-PLSR model showed the best results in the quantitative analysis of emulsified oil concentration. The coefficient of determination, mean square relative error, and ratio of performance to interquartile distance for the gasoline and diesel fuel emulsion validation sets were 0.93, 3.67%, 4.72, and 0.93, 3.72%, 4.60, respectively.

乳化油浓度是定量分析海面溢油污染的重要指标,建立快速有效的乳化油浓度定量分析方法对溢油量估算和溢油后评估具有重要作用。本文提出了一种基于激发-发射矩阵(EEM)荧光光谱和化学计量学的乳化油浓度定量分析方法。首先,使用 FLS1000 荧光光谱仪测量了两种乳化油的 EEM 荧光光谱。然后,利用并行因子分析(PARAFAC)对所测得的 EEM 荧光光谱进行分解,并从分解得到的载荷矩阵中筛选出几个关键的激发波长。随后,计算这些激发波长的三波段荧光指数(TBFI),并结合最佳波段选择算法,从中选出最佳发射波段组合。最后,将选定的最佳发射波段与偏最小二乘回归(PLSR)相结合,建立乳化油浓度预测模型。通过与基于 PARAFAC-PLSR 和多变量曲线解析-最小二乘(MCR-ALS)-PLSR 模型的预测结果进行比较,TBFI-PLSR 模型在乳化油浓度的定量分析中显示出最佳结果。汽油和柴油乳化液验证集的判定系数、均方相对误差和性能与四分位距之比分别为 0.93、3.67%、4.72 和 0.93、3.72%、4.60。
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引用次数: 0
A Green analytical method for simultaneous determination of dexamethasone sodium phosphate and prednisolone acetate in veterinary formulations using UV spectroscopy and dimension reduction algorithms. 利用紫外光谱和降维算法同时测定兽药配方中地塞米松磷酸钠和醋酸泼尼松龙的绿色分析方法。
Pub Date : 2024-11-16 DOI: 10.1016/j.saa.2024.125446
Marwan A Ibrahim, Ayman M Algohary, Youssef O Al-Ghamdi, Ahmed M Ibrahim

Precise determination of veterinary pharmaceutical concentrations represents a critical foundation for delivering safe and efficacious animal healthcare interventions. Two synthetic glucocorticoids - dexamethasone sodium phosphate (DXM) and prednisolone acetate (PRD) - are extensively employed in veterinary medicine due to their potent anti-inflammatory capabilities. Our research presents a novel, cost-effective, and environmentally sustainable analytical methodology that enables simultaneous quantification of DXM and PRD within binary veterinary formulations. The method synergistically combines UV spectroscopy with dimension reduction algorithms (DRAs), representing a significant advancement in pharmaceutical analysis. A comprehensive evaluation of seventeen DRAs was conducted using four distinct performance metrics: mean squared error (MSE), mean absolute error (MAE), median absolute error (MedAE), and coefficient of determination (R2). Among the assessed algorithms, mini-batch sparse principal component analysis demonstrated superior predictive accuracy for this specific analytical challenge. The developed method was validated using the accuracy profile approach, yielding results that confirm its satisfactory accuracy. An ecological impact assessment was conducted using five greenness evaluation tools: the Green Solvent Selection Tool (GSST), National Environmental Methods Index (NEMI), Green Certificate modified Eco-Scale, carbon footprint analysis, and the Modified GAPI (MoGAPI). In addition, whiteness was evaluated with Red-Green-Blue 12 (RGB 12) algorithms. The proposed method showed elevated GSST scores and a greener profile according to NEMI. The calculated carbon footprint was 0.0006 kg CO2 equivalent per sample, with a Green Certificate modified Eco-Scale score of 84, a MoGAPI score of 81, and a whiteness assessment of 90.1 by the RGB12 algorithm. Statistical comparison between the proposed spectrophotometric method and a previously reported HPLC method for pharmaceutical dosage form analysis revealed no statistically significant differences at the 95 % confidence level. This study underscores the innovative combination of UV spectroscopy with dimension reduction algorithms, presenting substantial improvements over traditional UV techniques for drug analysis. This method enhances both the efficiency and accuracy of active ingredient determination in pharmaceutical dosage forms while also supporting environmental sustainability.

精确测定兽药浓度是提供安全有效的动物保健干预措施的重要基础。两种合成糖皮质激素--地塞米松磷酸钠(DXM)和醋酸泼尼松龙(PRD)--因其强大的抗炎能力而被广泛应用于兽医领域。我们的研究提出了一种新颖、具有成本效益和环境可持续性的分析方法,可同时对二元兽药配方中的 DXM 和 PRD 进行定量。该方法将紫外光谱与降维算法(DRA)协同结合,是药物分析领域的一大进步。采用四种不同的性能指标对 17 种降维算法进行了全面评估:平均平方误差 (MSE)、平均绝对误差 (MAE)、中位数绝对误差 (MedAE) 和判定系数 (R2)。在所评估的算法中,微型批次稀疏主成分分析法在这一特定的分析挑战中表现出更高的预测准确性。使用准确性曲线方法对所开发的方法进行了验证,结果证实其准确性令人满意。使用五种绿色评价工具进行了生态影响评估:绿色溶剂选择工具(GSST)、国家环境方法指数(NEMI)、绿色证书修订生态尺度、碳足迹分析和修订 GAPI(MoGAPI)。此外,还采用红绿蓝 12 (RGB 12) 算法对白度进行了评估。根据 NEMI,建议的方法显示出更高的 GSST 分数和更绿色的轮廓。计算得出的每个样品的碳足迹为 0.0006 千克二氧化碳当量,绿色证书修改后的生态尺度得分为 84 分,MoGAPI 得分为 81 分,RGB12 算法的白度评估为 90.1 分。对拟议的分光光度法和之前报道的用于药物剂型分析的高效液相色谱法进行统计比较后发现,在 95% 的置信水平下,两者在统计上没有显著差异。这项研究强调了紫外光谱与降维算法的创新性结合,与传统的药物分析紫外技术相比有了实质性的改进。该方法提高了药物剂型中活性成分测定的效率和准确性,同时也支持环境的可持续发展。
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引用次数: 0
A Rhodamine-based high-sensitivity low-cytotoxicity probe for rapid turn-on detection of Hg2. 一种基于罗丹明的高灵敏度、低毒性探针,用于快速开启 Hg2 检测。
Pub Date : 2024-11-16 DOI: 10.1016/j.saa.2024.125443
Yongdi Liang, Xiaofeng Wang, Meixi Chen, Brian Musikavanhu, Attiq Ur Rehman, Xiuli Wang, Zhaoli Xue, Long Zhao

By integrating Rhodamine B and 4-phenylmorpholine moieties, a novel fluorescent probe named RhPy is synthesized for detecting Hg2+. Its recognition mechanism involves the reaction of Hg2+ with dithiooxamide, ultimately triggering the opening of the Rhodamine spirolactam and forming a new molecule RhPy-S with strong emission. The probe exhibits impressive limit of detection (0.015 μM) and short response time (<10 s). Importantly, RhPy shows almost none-cytotoxicity and RhPy-S has the emission spectrum peaking at 596 nm, which endow the probe with a good tissue penetration ability and practical utility in living cells, zebrafish and in vivo mice models. This work advances the field by providing a highly sensitive chemosensor for both environmental and biological applications.

通过整合罗丹明 B 和 4-苯基吗啉分子,合成了一种用于检测 Hg2+ 的新型荧光探针 RhPy。它的识别机制包括 Hg2+ 与二硫代草酰氨反应,最终引发罗丹明螺内酰胺打开,形成具有强发射的新分子 RhPy-S。该探针具有极高的检测限(0.015 μM)和较短的响应时间(0.015 μM)。
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引用次数: 0
Eco-friendly synchronous spectrofluorimetry coupled with chemometrics for simultaneous determination of ezetimibe and propranolol in pharmaceutical formulations and spiked plasma samples. 环保型同步分光荧光测定法与化学计量学相结合,用于同时测定药物制剂和加标血浆样品中的依折麦布和普萘洛尔。
Pub Date : 2024-11-16 DOI: 10.1016/j.saa.2024.125447
Ali Alqahtani, Taha Alqahtani, Adel Al Fatease, Adil Alshehri, Ahmed A Almrasy

In this study, synchronous fluorescence spectroscopic methods coupled with chemometric techniques were developed and evaluated for the simultaneous quantification of ezetimibe and propranolol, two commonly prescribed cardiovascular drugs. Both drugs exhibit overlapping native fluorescence, posing a challenge for their selective determination. To address this, chemometric models including partial least squares (PLS) and genetic algorithm-based variable selection (GA) were constructed using a calibration dataset based on a 52 factorial design resulting in 25 synthetic mixtures. The developed method has been optimized to account for factors such as solvent composition, micellar systems, and excitation/emission wavelengths that affect the fluorescence signals. The PLS and GA-PLS models were validated using an independent test set of 13 samples based on central composite design revealing the GA-PLS model provided improved quantitative performance with relative root mean square error of prediction (RRMSEP) values of 1.3939 and 1.0005 % for ezetimibe and propranolol, respectively, compared to 2.2502 and 2.3526 % for the PLS models. Hence, the GA-PLS models were successfully applied for the determination of ezetimibe and propranolol in pharmaceutical formulations and spiked plasma samples. Furthermore, the greenness and blueness of the proposed methods were compared against reported HPLC procedures using the AGREE and BAGI tools, revealing a greener analytical footprint for the developed method and higher analytical practicability posing as an environmental-friendly alternative to the standard HPLC technique.

本研究开发并评估了同步荧光光谱方法与化学计量学技术,用于同时定量分析依折麦布和普萘洛尔这两种常用的心血管药物。这两种药物的原生荧光相互重叠,给选择性测定带来了挑战。为了解决这个问题,我们利用基于 52 个因子设计的校准数据集构建了化学计量学模型,包括偏最小二乘法 (PLS) 和基于遗传算法的变量选择 (GA),从而得到 25 种合成混合物。对所开发的方法进行了优化,以考虑到溶剂成分、胶束系统和激发/发射波长等影响荧光信号的因素。使用基于中心复合设计的 13 个样本的独立测试集对 PLS 和 GA-PLS 模型进行了验证,结果表明 GA-PLS 模型的定量性能有所提高,对依折麦布和普萘洛尔的预测相对均方根误差(RRMSEP)值分别为 1.3939 % 和 1.0005 %,而 PLS 模型的预测相对均方根误差(RRMSEP)值分别为 2.2502 % 和 2.3526 %。因此,GA-PLS 模型成功地应用于药物制剂和血浆样品中依折麦布和普萘洛尔的测定。此外,利用 AGREE 和 BAGI 工具将所提出方法的绿色度和蓝色度与已报道的 HPLC 程序进行了比较,结果表明所开发方法的分析足迹更环保,分析实用性更高,是标准 HPLC 技术的环保型替代方法。
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引用次数: 0
期刊
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
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