Biochimica et biophysica acta最新文献

英文中文

On the active site of myosin A-adenosine triphosphatase IV. Properties of binding of trinitrobenzenesulfonate and p-chloromercuribenzoate to myosin a 肌球蛋白a -腺苷三磷酸酶活性位点的研究。三硝基苯磺酸盐和对氯苯甲磺酸盐与肌球蛋白a结合的性质

Biochimica et biophysica acta

Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91035-7

Yuji Tonomura , Junko Yoshimura, Toshiyuki Ohnishi

The addition of ATP or PP_i retarded conspicuously the rate of specific binding of trinitrobenzenesulfonate to one mole of lysine residue in 2.1·10⁵ g of myosin A. The rate of specific binding of trinitrobenzenesulfonate to myosin A was increased in 4M LiBr which melts almost completely the helical structure of myosin A. The rate was also remarkably influenced by the treatment of myosin A with 1.5 M LiBr which inactivated ATPase (ATP phosphohydrolase, EC 3.6.1.3) without changing significantly the helical content of the myosin A molecule as a whole. Furthermore, it was demonstrated that actomyosin reconstituted from myosin A treated with p-chloromercuribenzoate and β-mercaptoethanol does not show a clearing response on addition of high concentrations of ATP and its ATPase activity is not inhibited by the substrate or by EDTA. The p-chloromercuribenzotae added was completely removed from myosin A by the further addition of excess β-mercaptoethanol and the optical rotatory dispersion of myosin A was insignificantly altered by the treatment with p-chloromercuribenzoate and β-mercaptoethanol.

增加ATP或PPi弱智明显的特定绑定trinitrobenzenesulfonate一摩尔的赖氨酸残基105年2.1·克肌凝蛋白A特定绑定的trinitrobenzenesulfonate肌凝蛋白的速度增加4 M LiBr几乎完全融化的螺旋结构肌凝蛋白A率也显著影响肌凝蛋白的治疗与1.5 LiBr灭活的腺苷三磷酸酶(ATP phosphohydrolase,EC 3.6.1.3)，而不显著改变整个肌球蛋白A分子的螺旋含量。此外，研究表明，肌球蛋白A经对氯汞苯甲酸盐和β-巯基乙醇处理后重组的肌动球蛋白对高浓度ATP没有清除反应，其ATP酶活性不受底物或EDTA的抑制。加入过量的β-巯基乙醇后，肌球蛋白A中加入的对氯环苯甲醚被完全去除，对氯环苯甲酯和β-巯基乙醇对肌球蛋白A的旋光性影响不显著。

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引用次数: 19

Studies on the reaction between nicotinamide and iodoacetic acid 烟酰胺与碘乙酸反应的研究

Biochimica et biophysica acta

Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91041-2

D.S.R. Sarma, S. Rajalakshmi, P.S. Sarma

引用次数: 0

Binding of α-amylase to the cell wall of Aspergillus oryzae α-淀粉酶与米曲霉细胞壁的结合

Biochimica et biophysica acta

Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91068-0

Kenzo Tonomura, Fusae Futai, Osamu Tanabe

引用次数: 5

Protonenabhängige strukturönderungen im ferrihämoglobin

Biochimica et biophysica acta

Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91063-1

Hans-Dieter Ohlenbusch, Max Schlaak

引用次数: 1

An improved synthesis of phosphoenol pyruvate 丙酮酸磷酸烯醇的改进合成

Biochimica et biophysica acta

Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91040-0

V.M. Clark, A.J. Kirby

引用次数: 49

Binding of nystatin by mycoplasma (pleuropneumonia-like organisms) 支原体(胸膜肺炎样生物)结合制霉菌素

Biochimica et biophysica acta

Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91056-4

Shmuel Razin

引用次数: 10

The amino acid sequence of bovine heart cytochrome c 牛心脏细胞色素c的氨基酸序列

Biochimica et biophysica acta

Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91064-3

Kerry T. Yasunobu, Tadayoshi Nakashima, Harry Higa, Hiroshi Matsubara, Ann Benson

引用次数: 59

Hydroxyethylation of amino acids in plasma albumin with ethylene oxide 用环氧乙烷对血浆白蛋白中氨基酸的羟乙基化

Biochimica et biophysica acta

Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91025-4

Wesley C. Starbuck, Harris Busch

The reaction of ethylene oxide with amino acids in bovine plasma albumin was studied under various conditions of pH and time. Ethylene oxide reacted with arginyl, cystyl, histidyl, lysyl, methionyl and tyrosyl residues in albumin. The maximal reaction of arginyl, histidyl, lysyl and tyrosyl residues occurred at pH values equal to or greater than the pK of the respective reacting groups.

The reaction of ethylene oxide with the above amino acid residues resulted in the formation of 21 new peaks detected by chromatograpic analysis of the amino acids. The amino acids from which most of these products originated were detected by labeling the albumin biosynthetically with individual radioactive amino acids before hydroxyethylation. The major product of hydroxyethylation of lysine was apparently Nϵ[tris(2-hydroxyethyl)]lysine, since it corresponded to the Nϵ[tris(2-hydroxyethyl)]-lysine, prepared by direct synthesis, with respect to mobility in several chromatographic systems.

The accessibility of some lysyl and methionyl residues in albumin to ethylene oxide may be dependent upon a partial change in the tertiary structure of albumin, resulting from the reaction of cystyl residues in albumin with ethylene oxide.

研究了不同pH和时间条件下环氧乙烷与牛血浆白蛋白中氨基酸的反应。环氧乙烷与白蛋白中的精氨酸基、胱氨酸基、组氨酸基、赖氨酸基、蛋氨酸基和酪氨酸基残基反应。精氨酸基、组氨酸基、赖氨酸基和酪氨酸基残基的最大反应发生在pH值等于或大于各自反应基团的pK时。环氧乙烷与上述氨基酸残基反应形成了21个氨基酸色谱分析检测到的新峰。在羟基乙基化之前，用单个放射性氨基酸对白蛋白进行生物合成标记，检测了这些产物的氨基酸来源。赖氨酸羟乙基化的主要产物显然是n御[三(2-羟乙基)]赖氨酸，因为它对应于直接合成的n御[三(2-羟乙基)]-赖氨酸，在几种色谱系统中的迁移率。白蛋白中赖氨酸基和蛋氨酸基残基对环氧乙烷的接近性可能取决于白蛋白三级结构的部分改变，这是由白蛋白中胱氨酸残基与环氧乙烷反应引起的。

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引用次数: 9

Incomplete separation of human thyroglobulin from serum proteins by chromatography on diethylaminoethyl cellulose 二乙胺乙基纤维素层析法对人甲状腺球蛋白与血清蛋白的不完全分离

Biochimica et biophysica acta

Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91050-3

P.G. Stanley

引用次数: 3

La structure chimique du lysozyme de blanc d'oeuf de poule: étude détaillée 蛋清溶酶的化学结构:详细研究

Biochimica et biophysica acta

Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91033-3

Jacqueline Jollès, Juan Jauregui-Adell, Ida Bernier, Pierre Jollès

All the details concerning the establishment of the formula of the chemical structure of hen's egg-white lysozyme (N-acetylmuramide glycanohydrolase, EC 3.2.1.17) (which the authors had briefly indicated in 1961) are reported in the present study. The 18 tryptic units obtained from reduced lysozyme by chromatography on Dowex-1 X2 have beeb joined in the right order thanks to the chymotryptic units containing a basic amino acid and in two instances thanks to the peptic units. The protein is formed by a single polypeptide chain of 129 amino acid residues folded by the 4 bridges of the cystine residues; one of them has already been determined. Some considerations are made on the relations existing between chemical structure and biological activity.

本文报道了作者在1961年曾简要介绍过的蛋清溶菌酶(n -乙酰muramide glycanohydrolase, EC 3.2.1.17)化学结构公式的建立。通过Dowex-1 X2层析从还原溶菌酶中得到的18个胰酶单位，由于含有一个碱性氨基酸的胰酶单位，在两个例子中由于消化酶单位，它们以正确的顺序连接在一起。该蛋白由一条由129个氨基酸残基组成的单肽链由胱氨酸残基的4个桥折叠而成;其中一个已经确定了。对化学结构与生物活性之间存在的关系作了一些思考。

引用次数: 216

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