Biochimica et biophysica acta最新文献

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Study of [1-14C]glycine incorporation into extractable and residual glycogen of guinea-pig liver in vivo [1-14C]甘氨酸在豚鼠肝脏可提取和残留糖原中的掺入研究

Biochimica et biophysica acta

Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91045-X

Vishwa Nath Singh , T.A. Venkitasubramanian

引用次数: 0

Action de différents agents réducteurs sur le spectre, à basse température, de fractions subcellulaires de levure 不同还原剂对酵母亚细胞组分低温谱的影响

Biochimica et biophysica acta

Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91052-7

Thérèse Heyman-Blanchet

引用次数: 4

On the internal pH value of bacteria 细菌内部的pH值

Biochimica et biophysica acta

Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91053-9

Lawrence J. Kadish, Arthur B. Pardee

引用次数: 8

The effect of oxygen on the composition and organisation of the electron transport system of yeast 氧对酵母电子传递系统组成和组织的影响

Biochimica et biophysica acta

Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91062-X

D.R. Biggs, Anthony W. Linnane

引用次数: 33

The optical rotatory dispersion of myosin a IV. Conformational changes in meromyosins 肌凝蛋白的旋光色散。肌凝蛋白的构象变化

Biochimica et biophysica acta

Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91034-5

Yuji Tonomura , Kazuko Sekiya , Kiichi Imamura

The effect of 8% dioxane on the optical rotatory dispersion of myosin A purified by treatment with DEAE-cellulose was measured in 0.6 M KCl and at pH 7.0 and 20°. The increase in the —b₀ term by 5% was followed by a gradual decrease to 0.88 of the original. The —b₀ term of L₁-meromyosin in 0.6 M KCl and at pH 7.0 and 20% was 575 and was unchanged by the addition of dioxane, ATP of PP₁. On the other hand, the —b₀ term of H₂-meromyosin in 0.3 M KCl and at pH 7.0 and 20% was 263. It increased by several percent immediately after the addition of 8% dioxane and decreased gradually with time. It increased by several percent on the addition of 4 moles p-chloromercuribenzoate, but decreased by several percent on the addition of 8 moles p-chloromercuribenzoate per 10⁵ g protein. It was also decreased by the binding of H₂-meromyosin with F-actin. ATP increased the —b₀ term of H₂-meromyosin by several percent, though PP₁ decreased the term by several percent. Thus these reagents change the helical content of H₂-meromyosin in ways similar to the case of myosin A. The extents of the changes in the helical content of H₂-meromyosin by F-actin and ATP were, however, higher than those observed in myosin A.

在0.6 M KCl条件下，在pH 7.0和20°条件下，测定了8%二氧六环对deae -纤维素纯化的肌球蛋白A旋光性的影响。在- 0项增加5%之后，逐渐减少到原来的0.88。在0.6 M KCl和ph7.0和20%条件下，L1-meromyosin的-b0 term为575，并且随着PP1的ATP二氧六环的加入而保持不变。而在0.3 M KCl、7.0 pH和20%条件下，H2-meromyosin的-b0项为263。在加入8%二氧六环后，它立即上升几个百分点，并随着时间的推移逐渐下降。当每105g蛋白质中加入4mol对氯脲苯甲酸盐时，它增加了几个百分点，但当每105g蛋白质中加入8mol对氯脲苯甲酸盐时，它下降了几个百分点。H2-meromyosin与F-actin的结合也使其降低。ATP使h2 -肌凝蛋白的-b0期增加了几个百分点，而PP1使其减少了几个百分点。因此，这些试剂以类似于肌凝蛋白A的方式改变H2-meromyosin的螺旋含量。然而，F-actin和ATP对H2-meromyosin螺旋含量的改变程度高于在肌凝蛋白A中观察到的。

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引用次数: 12

Interaction of oestrogen quinones with ethylene diamine 雌激素醌类与乙二胺的相互作用

Biochimica et biophysica acta

Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91059-X

P.H. Jellinck, Louise Irwin

引用次数: 21

Changes in free and acetylated amino acids during tobacco mosaic virus multiplication 烟草花叶病毒增殖过程中游离氨基酸和乙酰化氨基酸的变化

Biochimica et biophysica acta

Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91030-8

Marvin Karasek

Changes in free and acetylated amino acids have been determined in control and infected tobacco plants during logarithmic tobacco mosaic virus multiplication. An increase in free amino acids occurs in infected tissue in the early stages of tobacco mosaic virus synthesis (72 and 144 h) followed by a decrease after 216 h. Acetylamino acids are not present in infected or in normal plant tissue in amounts that exceed 0.2 μmoles per 100 g of plant tissue. The induction of serine acetylase (acetyl-CoA: L-serine N-acetyltransferase) could not be demonstrated during logarithmic tobacco mosaic virus biosynthesis.

在对数烟草花叶病毒增殖过程中，测定了对照和侵染烟草植株游离氨基酸和乙酰化氨基酸的变化。在烟草花叶病毒合成的早期(72和144 h)，侵染组织中游离氨基酸增加，216 h后下降。在侵染组织和正常植物组织中，乙酰氨基酸的含量不超过0.2 μmol / 100 g。在对数烟草花叶病毒的生物合成过程中，不能证明丝氨酸乙酰化酶(乙酰辅酶a: l-丝氨酸n -乙酰转移酶)的诱导作用。

引用次数: 6

Changes in D-glucaric acid excretion induced by stimulators of ascorbic acid biosynthesis 抗坏血酸生物合成刺激剂诱导d -葡萄糖酸排泄的变化

Biochimica et biophysica acta

Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91038-2

C.A. Marsh, Lorna M. Reid

引用次数: 36

The effect of insulin on extracellular space and tissue-water content of the isolated rat diaphragm 胰岛素对离体大鼠横膈膜细胞外间隙及组织含水量的影响

Biochimica et biophysica acta

Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91057-6

G.R. Fritz, E. Knobil

引用次数: 11

Biosynthèse de l'arginine chez la levure I. Le sort de la Nα-acétylornithine 精氨酸在酵母中的生物合成I. Nα-乙酰氯硝胺的命运

Biochimica et biophysica acta

Pub Date : 1963-12-13 DOI: 10.1016/0006-3002(63)91026-6

R.H. De Deken

An enzyme, transferring the acetyl group of N-α-acetylornithine to the amino group of glutamate, has been demonstrated for Saccharomyces cerevisiae. On the other hand, the enzymic reduction of N-α-acetylglutamate with the formation of N-α-acetyl-glutamic-γ-semialdehyde has been reported previously. The absence of transacetylase in an arginine-requiring mutant (gene ar₇) as well as the above findings prove that:

1.
1. The biosynthesis of ornithine, in yeast, proceeds through acetylated intermediates.
2.
2. The transacetylase is the only functional pathway for the conversion of N-α-acetylornithine to ornithine, in spite of the simultaneous presence of an N-α-acetylornithinase (N-α-acetylornithine amidohydrolase).

At least two steps in the biosynthesis of ornithine are repressible by arginine.

一种能将N-α-乙酰鸟氨酸的乙酰基转化为谷氨酸的氨基的酶已被证实存在于酿酒酵母中。另一方面，N-α-乙酰谷氨酸的酶还原生成N-α-乙酰-谷氨酸-γ-半醛也有报道。需要精氨酸的突变体(ar7基因)中转乙酰化酶的缺失以及上述发现证明:1.1。鸟氨酸在酵母中的生物合成是通过乙酰化的中间体进行的。转乙酰化酶是将N-α-乙酰鸟氨酸转化为鸟氨酸的唯一功能途径，尽管同时存在N-α-乙酰鸟氨酸氨基水解酶。在鸟氨酸的生物合成过程中，至少有两个步骤受到精氨酸的抑制。

引用次数: 32

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