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The occurrence of cyst nematodes in agricultural fields of Southern Manitoba 马尼托巴省南部农田的胞囊线虫发生情况
IF 2 4区 农林科学 Q3 PLANT SCIENCES Pub Date : 2023-12-19 DOI: 10.1080/07060661.2023.2293128
M. Madani, Mario Tenuta, P. Castillo
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引用次数: 0
Species diversity of Fusarium head blight and deoxynivalenol (DON) levels in Western Canadian wheat fields 加拿大西部麦田头孢镰刀菌的物种多样性和脱氧雪腐镰刀菌烯醇(DON)水平
IF 2 4区 农林科学 Q3 PLANT SCIENCES Pub Date : 2023-12-19 DOI: 10.1080/07060661.2023.2290034
S. K. Kannangara, P. Bullock, S. Walkowiak, W. G. D. Fernando
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引用次数: 0
Metabarcoding for plant pathologists 植物病理学家的元标码
IF 2 4区 农林科学 Q3 PLANT SCIENCES Pub Date : 2023-12-15 DOI: 10.1080/07060661.2023.2290041
Martha A. Sudermann, Z. Foster, Jeff H. Chang, N. Grünwald
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引用次数: 0
Artificial intelligence analysis of contributive factors in determining blackleg disease severity in canola farmlands 人工智能分析确定油菜籽农田黑胫病严重程度的有利因素
IF 2 4区 农林科学 Q3 PLANT SCIENCES Pub Date : 2023-12-15 DOI: 10.1080/07060661.2023.2290039
Liang Zhao, Michael W. Harding, Gary Peng, Ralph Lange, S. Walkowiak, W. G. D. Fernando
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引用次数: 0
Some soilborne pathogens causing root rot and wilting of pistachio trees in southeastern Turkey 导致土耳其东南部开心果树根部腐烂和枯萎的一些土传病原体
IF 2 4区 农林科学 Q3 PLANT SCIENCES Pub Date : 2023-12-07 DOI: 10.1080/07060661.2023.2282549
Mehmet Hadi Aydin, Sirel Canpolat, Tuba Uzun
Soilborne fungal pathogens that cause root rot, wilting and dying are the most important problems in pistachio production. The purpose of this study was to examine, isolate, and diagnose the pathog...
土传真菌病原体会导致根部腐烂、枯萎和死亡,是开心果生产中最重要的问题。这项研究的目的是检查、分离和诊断土壤中的病原体。
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引用次数: 0
First report of yeast spot caused by Eremothecium coryli on soybean in South Korea 韩国首次报告由 Eremothecium coryli 在大豆上引起的酵母斑病
IF 2 4区 农林科学 Q3 PLANT SCIENCES Pub Date : 2023-12-06 DOI: 10.1080/07060661.2023.2287164
Okhee Choi, Haeun Noh, Yongsung Kang, Hyun Jin Chun, Min Chul Kim, Jinwoo Kim
An ascomycete yeast was isolated from tissue cultures of soybean explants (cv. Williams 82) exhibiting blanching and discoloration. Stink bug damage was confirmed in soybean seeds that were grown a...
从表现出褪色和变色的大豆(Williams 82 栽培品种)外植体组织培养物中分离出一种子囊酵母菌。从大豆种子中证实了臭虫的危害,这些种子是在...
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引用次数: 0
Symptomology, prevalence, and impact of Hop latent viroid on greenhouse-grown cannabis (Cannabis sativa L.) plants in Canada 加拿大温室种植大麻(大麻sativa L.)植物的症状学,流行率和啤酒花潜伏病毒的影响
IF 2 4区 农林科学 Q3 PLANT SCIENCES Pub Date : 2023-11-27 DOI: 10.1080/07060661.2023.2279184
Zamir K. Punja, Keri Wang, Samantha Lung, Liam Buirs
The incidence of Hop latent viroid (HLVd) affecting cannabis plants in licenced production facilities in Canada during 2020–2023 was determined by RT-PCR analyses of 15 947 samples from nine provin...
采用RT-PCR分析了来自加拿大9个省的15947份样本,确定了2020-2023年加拿大许可生产设施中大麻植物的啤酒花潜伏病毒(HLVd)发病率。
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引用次数: 0
Differential occurrence and strain composition of potato viruses between two major seed potato production zones in China suggest the need for different management strategies for the zones 中国两个主要种子马铃薯产区之间马铃薯病毒发生和品系组成的差异表明,需要采取不同的管理策略
IF 2 4区 农林科学 Q3 PLANT SCIENCES Pub Date : 2023-11-15 DOI: 10.1080/07060661.2023.2276224
Yanling Gao, Guoquan Fan, Shengqun Cheng, Wei Zhang, Yu Shen, Shu Zhang, Cailing Qiu, Xianzhou Nie, Yanju Bai, Wenhe Lu
Viral disease is one of the major reasons leading to yield and quality reduction of potato production. To gain better knowledge about seed potato quality in China, the occurrences of six common vir...
病毒病是马铃薯产量和品质下降的主要原因之一。为了更好地了解中国的种薯质量,我们对6种常见的马铃薯病害进行了分析。
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引用次数: 0
Comparative proteomic analysis of the moderately resistant and susceptible peanut cultivars during infestation by Cylindrocladium parasiticum 中抗和易感花生品种在筒蛾侵染期间的蛋白质组学比较分析
4区 农林科学 Q3 PLANT SCIENCES Pub Date : 2023-11-14 DOI: 10.1080/07060661.2023.2274327
Xinyu Chen, Mei Luo, Meimei Xiang, Lina Xiong, Yongxin Shu, Guohui Yu, Zhangyong Dong, Yunhao Sun
AbstractCylindrocladium parasiticum Crous, Wingfield & Alfenas, the causal agent of Cylindrocladium black rot of peanut, has led to economic losses in China. However, the response of peanut plant to C. parasiticum is poorly understood. Here, two contrasting peanut cultivars, T09 (C. parasiticum-moderately resistant) and P562 (C. parasiticum-susceptible) were used for comparative analysis of protein profiles in root segment of plant tissues in responses to C. parasiticum infection. Proteomic profiling identified 1647 and 391 differentially accumulated proteins (DAPs) in P562 and T09 relative to uninoculated control, respectively. A total of 350 and 1095 DAPs were identified between P562 and T09 after 9 day(s) post infection (dpi), respectively. Functional categorization showed that C. parasiticum-responsive proteins were mainly involved in catalytic activity and binding functions. KEGG pathway analysis indicated both moderately resistant and susceptible peanut cultivars can regulate gene expression in the phenylpropanoid pathway, terpenoid backbone biosynthesis, SA, and JA pathways to induce defensive genes and protein expression which enhances plant defence or susceptibility capacity. However, MAPK signal pathway was more pronounced in moderately resistant peanut cultivar T09. We also observed an increase of CYP73A100 involved in phenylpropanoid and flavonoid biosynthesis pathways in susceptible peanut ecotype P562, while decrease in moderately resistant interaction. Additionally, there was a marked activation of brassinosteroid biosynthesis in T09, which indicated a possible involvement of activation of plant immune response in moderately resistant responses of peanut to C. parasiticum. This study provides some insights into molecular networks involved in cellular and physiological responses to C. parasiticum in peanut.RésuméCylindrocladium parasiticum Crous, Wingfield & Alfenas, l’agent causal de la pourriture noire à Cylindrocladium de l’arachide (Arachis hypogaea), est un champignon qui infecte les racines, causant ainsi d’importantes pertes économiques en Chine, aux États-Unis et ailleurs dans le monde. Toutefois, les réactions physiologiques et biochimiques des plants d’arachides à C. parasiticum sont mal comprises. Nous avons étudié, dans le cadre de cette recherche, deux cultivars de plants d’arachides dont la sensibilité à l’infection causée par C.parasiticum varie, T09 (modérément résistant) et P562 (sensible), en nous basant sur l’analyse protéomique comparative de segments de racines à la suite de l’infection provoquée par C. parasiticum. Nous avons identifié 1 647 et 391 protéines différentiellement abondantes (PDA) chez les plants infectés de P562 et de T09, respectivement, comparativement aux plants témoins non inoculés. Nous avons également identifié 350 et 1 095 PDA chez les plants inoculés et non inoculés de P562 et de T09, et ce, 9 jours après l’infection. La catégorisation fonctionnelle des PDA a montré que les protéines sensi
【摘要】花生黑腐病的病原是花生黑腐病的主要病原菌——克罗斯、Wingfield和Alfenas。然而,花生植株对寄生蜂的反应尚不清楚。以2个花生品种T09(中度抗)和P562(敏感)为研究对象,比较分析了花生对寄生蜂侵染反应的根系蛋白谱。蛋白质组学分析发现,与未接种对照相比,P562和T09分别有1647和391个差异积累蛋白(DAPs)。感染后9天(dpi), P562和T09分别鉴定出350和1095个dap。功能分类表明,寄生蜂应答蛋白主要参与催化活性和结合功能。KEGG途径分析表明,中抗性和易感花生品种均可通过调控苯丙素途径、萜类主干生物合成途径、SA和JA等途径的基因表达,诱导防御基因和蛋白表达,增强植株的防御或易感能力。而MAPK信号通路在中抗性花生品种T09中更为明显。我们还观察到,在花生易感型P562中,参与苯丙素和类黄酮生物合成途径的CYP73A100增加,而在中度抗性相互作用中减少。此外,在T09中,油菜素内酯的生物合成被显著激活,这表明花生对寄生蜂的中度抗性反应可能参与了植物免疫应答的激活。本研究为花生对寄生蜂的细胞和生理反应的分子网络提供了一些新的认识。ransamsamicadium parasiticum Crous, Wingfield & Alfenas, l 'agent causal de la pourcure noire (Arachis hypogaea), est un champignon qui感染的疫苗,causant ainsi 'importantes peres在中国的<s:1> <s:1> (États-Unis), et ailleurs dans le monde。昆虫,昆虫,昆虫,昆虫,昆虫,昆虫,昆虫等。Nous avons <s:2>, dans le cadre de ette recherche,两个品种的植物d 'arachides不具有敏感性,<s:2> '感染引起的csm .parasiticum变种,T09 (modsamacimement, csm .抗)和P562 (sensible),甚至Nous basant sur l 'analyse prosamomique de segments de racines, <s:2> la suite de感染引起的csm .parasiticum。Nous avons分别鉴定了1 647和391种丰富的蛋白质与植物感染的P562和T09的不同,比较了植物感染的P562和T09的不同。Nous avons对350株和1095株植物的感染进行了鉴定,这些植物接种了P562和T09的感染和非接种了P562和T09的感染。寄生c.p atticum sonsonprinciple隐含的原理,即:<s:2> <s:2> <s:2>的变性变性变性,<s:2> <s:2>的变性变性,<s:2> <s:2>的变性变性,<s:2> <s:2>的变性,<s:2>的变性,<s:2>的变性,<s:2>的变性,<s:2>的变性,<s:2>的变性,<s:2>的变性,<s:2>的变性,<s:2>的变性,<s:2>的变性,<s:2>的变性,<s:2>的变性,<s:2>的变性,<s:2>的变性,<s:2>的变性,<s:2>的变性,<s:2>的变性,<s:2>的变性,<s:2>的变性,以及植物的变性。lavoie de signalisation MAPK a semblblous jouer un rôle加上marqueise chechele栽培的modsamr2013.2013.03。复仇,le品种合理P562 affichait des taux + eleves de CYP73A100联合国细胞色素P450 implique在biosynthese des phenilpropanoides et des黄酮类,缆车品种T09。<s:1> <s:1> <s:1>生物合成<e:1>与<s:1> <s:1>生物合成<e:1>与<s:1> <s:1>生物合成<e:1>与<s:1> <s:1>生物合成与<s:1>生物合成与<s:1>生物合成与<s:1>生物合成与<s:1>生物合成与<s:1>生物合成与生物合成与<s:1>生物合成与生物合成与生物合成与生物合成与个体激活的可能性。catia的研究表明:catia的研究表明:catia的研究表明:catia的研究表明:catia的研究表明:catia的研究表明:catia的研究表明:catia的研究表明:catia的研究表明:catia的研究表明:catia的研究表明:catia的研究表明:catia的研究表明:catia的研究表明:catia的研究表明:catia的研究表明:catia的研究表明:catia的研究表明:catia的研究表明:catia的研究表明:关键词:寄生蜂;细胞和生理反应;蛋白质组;感谢仲恺农业工程大学郑义雄教授提供花生品系T09和P562。披露声明作者未报告潜在的利益冲突。作者贡献:李洪志和张志勇构思并监督了这个项目,李洪志、张志勇和张志勇设计了实验。X.Y.C.做了大部分的实验。m.l.、M.M.X、L.N.X、Y.X.S和G.H.Y.做了一些实验。y.h.s., z.y.d., x.y.c.和L.N.X.分析了数据并撰写了手稿。质谱蛋白质组学数据已通过PRIDE合作伙伴存储库存储到ProteomeXchange Consortium,数据集标识符为PXD024578。https://www.ebi.ac。
{"title":"Comparative proteomic analysis of the moderately resistant and susceptible peanut cultivars during infestation by <i>Cylindrocladium parasiticum</i>","authors":"Xinyu Chen, Mei Luo, Meimei Xiang, Lina Xiong, Yongxin Shu, Guohui Yu, Zhangyong Dong, Yunhao Sun","doi":"10.1080/07060661.2023.2274327","DOIUrl":"https://doi.org/10.1080/07060661.2023.2274327","url":null,"abstract":"AbstractCylindrocladium parasiticum Crous, Wingfield & Alfenas, the causal agent of Cylindrocladium black rot of peanut, has led to economic losses in China. However, the response of peanut plant to C. parasiticum is poorly understood. Here, two contrasting peanut cultivars, T09 (C. parasiticum-moderately resistant) and P562 (C. parasiticum-susceptible) were used for comparative analysis of protein profiles in root segment of plant tissues in responses to C. parasiticum infection. Proteomic profiling identified 1647 and 391 differentially accumulated proteins (DAPs) in P562 and T09 relative to uninoculated control, respectively. A total of 350 and 1095 DAPs were identified between P562 and T09 after 9 day(s) post infection (dpi), respectively. Functional categorization showed that C. parasiticum-responsive proteins were mainly involved in catalytic activity and binding functions. KEGG pathway analysis indicated both moderately resistant and susceptible peanut cultivars can regulate gene expression in the phenylpropanoid pathway, terpenoid backbone biosynthesis, SA, and JA pathways to induce defensive genes and protein expression which enhances plant defence or susceptibility capacity. However, MAPK signal pathway was more pronounced in moderately resistant peanut cultivar T09. We also observed an increase of CYP73A100 involved in phenylpropanoid and flavonoid biosynthesis pathways in susceptible peanut ecotype P562, while decrease in moderately resistant interaction. Additionally, there was a marked activation of brassinosteroid biosynthesis in T09, which indicated a possible involvement of activation of plant immune response in moderately resistant responses of peanut to C. parasiticum. This study provides some insights into molecular networks involved in cellular and physiological responses to C. parasiticum in peanut.RésuméCylindrocladium parasiticum Crous, Wingfield & Alfenas, l’agent causal de la pourriture noire à Cylindrocladium de l’arachide (Arachis hypogaea), est un champignon qui infecte les racines, causant ainsi d’importantes pertes économiques en Chine, aux États-Unis et ailleurs dans le monde. Toutefois, les réactions physiologiques et biochimiques des plants d’arachides à C. parasiticum sont mal comprises. Nous avons étudié, dans le cadre de cette recherche, deux cultivars de plants d’arachides dont la sensibilité à l’infection causée par C.parasiticum varie, T09 (modérément résistant) et P562 (sensible), en nous basant sur l’analyse protéomique comparative de segments de racines à la suite de l’infection provoquée par C. parasiticum. Nous avons identifié 1 647 et 391 protéines différentiellement abondantes (PDA) chez les plants infectés de P562 et de T09, respectivement, comparativement aux plants témoins non inoculés. Nous avons également identifié 350 et 1 095 PDA chez les plants inoculés et non inoculés de P562 et de T09, et ce, 9 jours après l’infection. La catégorisation fonctionnelle des PDA a montré que les protéines sensi","PeriodicalId":9468,"journal":{"name":"Canadian Journal of Plant Pathology","volume":"28 25","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134954476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Onion leaf blight caused by Alternaria alternata sensu lato and Stemphylium vesicarium in Michoacán, México Michoacán、m<s:1>、xco .洋葱叶枯病
4区 农林科学 Q3 PLANT SCIENCES Pub Date : 2023-11-03 DOI: 10.1080/07060661.2023.2277819
Alfredo Reyes-Tena, Amelia Cristina Montoya-Martínez, Sylvia Patricia Fernández-Pavía, Ricardo Santillán-Mendoza, Adanely Jiménez-Villegas, Daniela Pineda-Vaca, Gerardo Rodríguez-Alvarado
AbstractMéxico is among the main producers of onions, which are cultivated mainly in the states of Baja California, Chihuahua, Guanajuato, Michoacán, and Zacatecas. During June and November 2017, foliar blight symptoms were observed in commercial onion crops in the municipality of Copándaro, Michoacán. The incidence of the disease was 20% in June and 80% in November. The objective of this work was to identify the causal agent or agents of this disease. Fungal isolates recovered from symptomatic plants presented conidia typical of Alternaria and Stemphylium. Molecular identification and phylogenetic analysis using partial sequences of the nuclear ribosomal internal transcribed spacer (ITS) region, the translation elongation factor-1 alpha (TEF1), and RNA polymerase subunit II (RPB2) genes confirmed the identity of the isolates as species within the Alternaria alternata species complex, and Stemphylium vesicarium. The isolates reproduced foliar blight symptoms in inoculated onion plants in pathogenicity tests. This is the first report of Alternaria alternata sensu lato and Stemphylium vesicarium causing foliar blight in commercial onion crops in Michoacán.Le Mexique est un des principaux producteurs d’oignons, ceux-ci étant cultivés dans les États de Basse-Californie, de Chihuahua, de Guanajuato, de Michoacán et de Zacatecas. Au cours de juin et de novembre 2017, des symptômes de brûlure helminthosporienne ont été observés dans les cultures commerciales de la municipalité de Copándaro, dans le Michoacán. L’incidence de la maladie était de 20 % en juin et de 80 % en novembre. Le but de cette étude était d’identifier l’agent ou les agents causaux de cette maladie. Des isolats fongiques provenant de plants symptomatiques comportaient les conidies typiques d’Alternaria et de Stemphylium. L’identification moléculaire et l’analyse phylogénétique basée sur des séquences partielles de la région de l’espaceur transcrit interne (ITS) de l’ADN ribosomique nucléaire, le facteur d’élongation de la traduction-1 alpha (TEF1) et les gènes de la sous-unité II de l’ARN polymérase (RPB2) ont confirmé l’identité des isolats en tant qu’espèces du complexe Alternaria alternata et Stemphylium vesicarium. Les isolats ont reproduit les symptômes de la brûlure helminthosporienne chez les plants d’oignons inoculés dans le cadre de tests de pathogénicité. Il s’agit de la première mention d’Alternaria alternata et de Stemphylium vesicarium causant la brûlure helminthosporienne dans des cultures commerciales d’oignons au Michoacán.Keywords: Allium cepaKoch’s postulatesleaf blightmultilocus phylogenypathogenicityKEYWORDS: Mots clésAllium cepabrûlure helminthosporiennepathogénicitéphylogénie multilocuspostulats de KochDisclaimerAs a service to authors and researchers we are providing this version of an accepted manuscript (AM). Copyediting, typesetting, and review of the resulting proofs will be undertaken on this manuscript before final publication of the Version of Record (VoR)
墨西哥是洋葱的主要生产商之一,洋葱主要种植在下加利福尼亚州、奇瓦瓦州、瓜纳华托州、Michoacán和萨卡特卡斯州。2017年6月和11月,在Copándaro和Michoacán市的商品洋葱作物中观察到叶枯病症状。6月和11月的发病率分别为20%和80%。这项工作的目的是确定这种疾病的致病因子。从有症状植物中分离的真菌具有典型的互交菌和茎孢菌的分生孢子。利用核糖体内部转录间隔区(ITS)、翻译延伸因子-1 α (TEF1)和RNA聚合酶亚基II (RPB2)基因的部分序列进行分子鉴定和系统发育分析,证实了分离物属于互花孢属(Alternaria alternata)种复合体和水仙花(Stemphylium vesicarium)种。病原菌在接种洋葱植株上重现叶枯病症状。本文是Michoacán地区首次报道了引起洋葱叶枯病的洋葱互花霉和洋葱茎霉。墨西哥的主要生产地是墨西哥、墨西哥、墨西哥、墨西哥、墨西哥、墨西哥、墨西哥、墨西哥、墨西哥、墨西哥和墨西哥。在2017年6月和11月的课程中,des symptômes de br引诱helminthosporienne和观察与les culculescommerciales de la municipalit Copándaro, dans le Michoacán。6月和11月的发病率分别为20%和80%。但是,如果你的病是由你的病引起的,你的病是由你的病引起的。该菌株分离了植物病原性症状,并与典型的交替病和茎类疾病相结合。L 'identification moleculaire等分析phylogenetique basee苏尔des序列partielles de la地区de L 'espaceur transcrit实习医师(其)de L 'ADN ribosomique远离,le因素d 'elongation de la traduction-1α(TEF1) et de la sous-unite les基因2 de L 'ARN聚合酶(RPB2)证实L 'identite des isolats en瞿如此更'especes du complexe主产et Stemphylium vesicarium。分离株不复制株symptômes de la br, helminthosporienne,植物d 'oignons inocul,干系株de tests de pathogsamnicit。我将会在《植物互生线虫》中提到《植物互生线虫》和《植物茎孢病菌》,并在《植物互生线虫》中提到《植物互生线虫》。关键词:葱属植物cepaKoch的postulatesleaf blightmultilocus phylogenypathogenicityKEYWORDS:著clesAllium cepabrulure helminthosporiennepathogenicitephylogenie multilocuspostulats de KochDisclaimerAs作者和研究人员服务我们提供这个版本的一个公认的手稿(AM)。在最终出版版本记录(VoR)之前,将对该手稿进行编辑、排版和审查。在制作和印前,可能会发现可能影响内容的错误,所有适用于期刊的法律免责声明也与这些版本有关。作者感谢Marlene Díaz-Celaya在分离和开发致病性测试过程中的后勤支持。数据可用性声明本研究中分析的分离株可在CM-CNRG (Colección de Microorganismos, Centro Nacional de Recursos gen, Jalisco, m xico)和INIFAP (Instituto Nacional de investigacones Forestales, Agrícolas y Pecuarias) (https://vun.inifap.gob.mx/portalweb/_Centros?C=007)获得。生成的DNA序列可在国家生物技术信息中心(https://www.ncbi.nlm.nih.gov/)的GenBank中获得,登录号为MN596827-MN596834, MW429366, MW429367, ON745744-ON745746, ON859132, ON859133, ON873736, ON873737, ON911543-ON911545。
{"title":"Onion leaf blight caused by <i>Alternaria alternata sensu lato</i> and <i>Stemphylium vesicarium</i> in Michoacán, México","authors":"Alfredo Reyes-Tena, Amelia Cristina Montoya-Martínez, Sylvia Patricia Fernández-Pavía, Ricardo Santillán-Mendoza, Adanely Jiménez-Villegas, Daniela Pineda-Vaca, Gerardo Rodríguez-Alvarado","doi":"10.1080/07060661.2023.2277819","DOIUrl":"https://doi.org/10.1080/07060661.2023.2277819","url":null,"abstract":"AbstractMéxico is among the main producers of onions, which are cultivated mainly in the states of Baja California, Chihuahua, Guanajuato, Michoacán, and Zacatecas. During June and November 2017, foliar blight symptoms were observed in commercial onion crops in the municipality of Copándaro, Michoacán. The incidence of the disease was 20% in June and 80% in November. The objective of this work was to identify the causal agent or agents of this disease. Fungal isolates recovered from symptomatic plants presented conidia typical of Alternaria and Stemphylium. Molecular identification and phylogenetic analysis using partial sequences of the nuclear ribosomal internal transcribed spacer (ITS) region, the translation elongation factor-1 alpha (TEF1), and RNA polymerase subunit II (RPB2) genes confirmed the identity of the isolates as species within the Alternaria alternata species complex, and Stemphylium vesicarium. The isolates reproduced foliar blight symptoms in inoculated onion plants in pathogenicity tests. This is the first report of Alternaria alternata sensu lato and Stemphylium vesicarium causing foliar blight in commercial onion crops in Michoacán.Le Mexique est un des principaux producteurs d’oignons, ceux-ci étant cultivés dans les États de Basse-Californie, de Chihuahua, de Guanajuato, de Michoacán et de Zacatecas. Au cours de juin et de novembre 2017, des symptômes de brûlure helminthosporienne ont été observés dans les cultures commerciales de la municipalité de Copándaro, dans le Michoacán. L’incidence de la maladie était de 20 % en juin et de 80 % en novembre. Le but de cette étude était d’identifier l’agent ou les agents causaux de cette maladie. Des isolats fongiques provenant de plants symptomatiques comportaient les conidies typiques d’Alternaria et de Stemphylium. L’identification moléculaire et l’analyse phylogénétique basée sur des séquences partielles de la région de l’espaceur transcrit interne (ITS) de l’ADN ribosomique nucléaire, le facteur d’élongation de la traduction-1 alpha (TEF1) et les gènes de la sous-unité II de l’ARN polymérase (RPB2) ont confirmé l’identité des isolats en tant qu’espèces du complexe Alternaria alternata et Stemphylium vesicarium. Les isolats ont reproduit les symptômes de la brûlure helminthosporienne chez les plants d’oignons inoculés dans le cadre de tests de pathogénicité. Il s’agit de la première mention d’Alternaria alternata et de Stemphylium vesicarium causant la brûlure helminthosporienne dans des cultures commerciales d’oignons au Michoacán.Keywords: Allium cepaKoch’s postulatesleaf blightmultilocus phylogenypathogenicityKEYWORDS: Mots clésAllium cepabrûlure helminthosporiennepathogénicitéphylogénie multilocuspostulats de KochDisclaimerAs a service to authors and researchers we are providing this version of an accepted manuscript (AM). Copyediting, typesetting, and review of the resulting proofs will be undertaken on this manuscript before final publication of the Version of Record (VoR)","PeriodicalId":9468,"journal":{"name":"Canadian Journal of Plant Pathology","volume":"41 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135819604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Canadian Journal of Plant Pathology
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