Pub Date : 2023-06-14DOI: 10.1080/07060661.2023.2221652
Mahboobeh Yazdani, M. Yassaie, S. Rezaee, Abodol Karim Zakeri, M. Patpour
Abstract Puccinia striiformis Westend. f. sp. tritici Eriksson (Pst), the causal agent of stripe rust, is known as an important and destructive pathogen that causes critical wheat losses in many parts of the world. Employing effective resistant genotypes is a safe and operative strategy to combat the disease. Iran is located in the Fertile Crescent, the co-epicentre of wheat and its biotic stresses. Iranian wheat landraces may carry important sources of resistance against rust diseases. Adult plant reactions of 467 Iranian wheat landraces to stripe rust were studied in two cropping seasons in Fars province. One hundred and six landraces showed acceptable levels of resistance and were selected for further seedling and molecular studies based on their lower infection type, coefficient of infection (CI) and average coefficient of infection (ACI). The presence of Yr4, Yr10, Yr24 and Yr32 resistance genes were postulated using 10 different Pst isolates including PstS10, PstS7, PstS3, PstS2+V27, PstS11, PstS8, PstS13, PstS6, PstS0 and Me2018 under greenhouse conditions. Field and greenhouse reactions of near-isogenic lines reconfirmed the presence of the studied Yr resistance genes in 20 genotypes. Moreover, Xcfb3530 150 , Xpsp3000, Xgwm11, and Xwmc198 SSR markers were employed to identify and validate the presence of the above corresponding genes. The presence of Yr4, Yr10, Yr24 and Yr32 resistance genes in five (1.07%), 10 (2.14%), five (1.07%) and seven (1.5%) experimental landraces were confirmed, respectively. Six genotypes are harbouring more than one resistance gene. These effective resistance sources can be employed in national and international breeding programs.
{"title":"Exploring Iranian wheat landraces for stripe rust resistance genes and validation of selected genes using molecular markers","authors":"Mahboobeh Yazdani, M. Yassaie, S. Rezaee, Abodol Karim Zakeri, M. Patpour","doi":"10.1080/07060661.2023.2221652","DOIUrl":"https://doi.org/10.1080/07060661.2023.2221652","url":null,"abstract":"Abstract Puccinia striiformis Westend. f. sp. tritici Eriksson (Pst), the causal agent of stripe rust, is known as an important and destructive pathogen that causes critical wheat losses in many parts of the world. Employing effective resistant genotypes is a safe and operative strategy to combat the disease. Iran is located in the Fertile Crescent, the co-epicentre of wheat and its biotic stresses. Iranian wheat landraces may carry important sources of resistance against rust diseases. Adult plant reactions of 467 Iranian wheat landraces to stripe rust were studied in two cropping seasons in Fars province. One hundred and six landraces showed acceptable levels of resistance and were selected for further seedling and molecular studies based on their lower infection type, coefficient of infection (CI) and average coefficient of infection (ACI). The presence of Yr4, Yr10, Yr24 and Yr32 resistance genes were postulated using 10 different Pst isolates including PstS10, PstS7, PstS3, PstS2+V27, PstS11, PstS8, PstS13, PstS6, PstS0 and Me2018 under greenhouse conditions. Field and greenhouse reactions of near-isogenic lines reconfirmed the presence of the studied Yr resistance genes in 20 genotypes. Moreover, Xcfb3530 150 , Xpsp3000, Xgwm11, and Xwmc198 SSR markers were employed to identify and validate the presence of the above corresponding genes. The presence of Yr4, Yr10, Yr24 and Yr32 resistance genes in five (1.07%), 10 (2.14%), five (1.07%) and seven (1.5%) experimental landraces were confirmed, respectively. Six genotypes are harbouring more than one resistance gene. These effective resistance sources can be employed in national and international breeding programs.","PeriodicalId":9468,"journal":{"name":"Canadian Journal of Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45229639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-19DOI: 10.1080/07060661.2023.2215212
F. Wani, Shahjahan Rashid, Sumiah Wani, Sahar Saleem Bhat, Sanober Bhat, Ebru Derelli Tufekci, A. El Sabagh, S. H. Wani, Aflaq Hamid
Abstract Plant viruses infect a wide variety of economically important crop plants and cause significant loss in agricultural production around the world. Conventional control strategies are insufficient to combat rapidly evolving plant viruses. In recent years, genome editing technologies have paved new ways for manipulating viral genomes (DNA or RNA). Among them, the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) system has been seen to be able to engineer plant virus resistance by directly targeting the viral genome as well as by inactivating host susceptibility genes. In this review, we survey genome editing tools targeting viral genomes, with an emphasis on CRISPR/Cas9. The advantages of the CRISPR/Cas9 system for combating plant viruses as well as its limitations are discussed in detail.
{"title":"Applications of Genome Editing in Plant Virus Disease Management: CRISPR/Cas9 Plays a Central Role","authors":"F. Wani, Shahjahan Rashid, Sumiah Wani, Sahar Saleem Bhat, Sanober Bhat, Ebru Derelli Tufekci, A. El Sabagh, S. H. Wani, Aflaq Hamid","doi":"10.1080/07060661.2023.2215212","DOIUrl":"https://doi.org/10.1080/07060661.2023.2215212","url":null,"abstract":"Abstract Plant viruses infect a wide variety of economically important crop plants and cause significant loss in agricultural production around the world. Conventional control strategies are insufficient to combat rapidly evolving plant viruses. In recent years, genome editing technologies have paved new ways for manipulating viral genomes (DNA or RNA). Among them, the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) system has been seen to be able to engineer plant virus resistance by directly targeting the viral genome as well as by inactivating host susceptibility genes. In this review, we survey genome editing tools targeting viral genomes, with an emphasis on CRISPR/Cas9. The advantages of the CRISPR/Cas9 system for combating plant viruses as well as its limitations are discussed in detail.","PeriodicalId":9468,"journal":{"name":"Canadian Journal of Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43918141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-17DOI: 10.1080/07060661.2023.2215208
N. Afshan, Saliha Afzal, M. Riaz, Arooma Saleem, A. R. Niazi, A. Khalid
Abstract Leaves of purple buckthorn observed with powdery mildew symptoms were collected from the district Abbottabad, Khyber-Pakhtunkhwa, Pakistan during field tours from August to October between 2017 and 2021. Morpho-anatomical features and molecular phylogenetic analysis were used to identify the causal agent as Erysiphe friesii var. dahurica. Pathogenicity was confirmed on healthy leaves of Rhamnus purpurea in a glasshouse at 25°C with 75 to 80% humidity and after inoculation, white powdery colonies appeared after 7 to 10 days. The fungal colonies were checked for the confirmation of pathogen. Erysiphe friesii var. dahurica on R. purpurea is being described here as a new record for Pakistan and Rhamnus purpurea is a new host, reported for the first time.
{"title":"Occurrence of Erysiphe friesii var. dahurica on purple buckthorn (Rhamnus purpurea) from Pakistan","authors":"N. Afshan, Saliha Afzal, M. Riaz, Arooma Saleem, A. R. Niazi, A. Khalid","doi":"10.1080/07060661.2023.2215208","DOIUrl":"https://doi.org/10.1080/07060661.2023.2215208","url":null,"abstract":"Abstract Leaves of purple buckthorn observed with powdery mildew symptoms were collected from the district Abbottabad, Khyber-Pakhtunkhwa, Pakistan during field tours from August to October between 2017 and 2021. Morpho-anatomical features and molecular phylogenetic analysis were used to identify the causal agent as Erysiphe friesii var. dahurica. Pathogenicity was confirmed on healthy leaves of Rhamnus purpurea in a glasshouse at 25°C with 75 to 80% humidity and after inoculation, white powdery colonies appeared after 7 to 10 days. The fungal colonies were checked for the confirmation of pathogen. Erysiphe friesii var. dahurica on R. purpurea is being described here as a new record for Pakistan and Rhamnus purpurea is a new host, reported for the first time.","PeriodicalId":9468,"journal":{"name":"Canadian Journal of Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48176602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-11DOI: 10.1080/07060661.2023.2206378
Rosa E. Prahl, Shahjahan Khan, R. Deo
Abstract This review paper highlights the significant research conducted on fungi belonging to the genus Ampelomyces. Phylogeny based on both ITS and actin sequences has grouped Ampelomyces into different lineages. However, the ITS2 spacer, one constituent of the ITS region, together with their secondary structures (S2s), showed that these lineages are represented by different S2s; also, evidence of pseudogene formation in nuclear ribosomal genes of two isolates was reported, and S2s in Ampelomyces mycoparasites are different from those in Phoma-like fungi. Ampelomyces taxonomy is unresolved and future multi-locus analysis will assist in delimiting species. Members of the genus Ampelomyces are among the first mycoparasites used to control powdery mildew fungi as they can efficiently eliminate mycelial growth and reduce the overwintering inoculum of their mycohosts. In addition, Ampelomyces isolates were found to be resistant to some fungicides and insecticides, e.g. pyrazophos, an attractive feature for their selection as biocontrol agents. Transcriptome analyses have revealed that expression of the genes that encode proteins putatively associated with virulence and plant immune responses were enhanced during host recognition, while genes-encoding proteins linked to antibiotic resistance were predicted within the Ampelomyces genome. Proteomic studies are needed to confirm whether these proteins function in virulence and can therefore be used for biocontrol purposes or as bacterial antibiotic-resistant proteins, and which of these may trigger plant immune responses to facilitate plant protection. We encourage the continuation of these studies to benefit crop protection research.
{"title":"Ampelomyces mycoparasites of powdery mildews – a review","authors":"Rosa E. Prahl, Shahjahan Khan, R. Deo","doi":"10.1080/07060661.2023.2206378","DOIUrl":"https://doi.org/10.1080/07060661.2023.2206378","url":null,"abstract":"Abstract This review paper highlights the significant research conducted on fungi belonging to the genus Ampelomyces. Phylogeny based on both ITS and actin sequences has grouped Ampelomyces into different lineages. However, the ITS2 spacer, one constituent of the ITS region, together with their secondary structures (S2s), showed that these lineages are represented by different S2s; also, evidence of pseudogene formation in nuclear ribosomal genes of two isolates was reported, and S2s in Ampelomyces mycoparasites are different from those in Phoma-like fungi. Ampelomyces taxonomy is unresolved and future multi-locus analysis will assist in delimiting species. Members of the genus Ampelomyces are among the first mycoparasites used to control powdery mildew fungi as they can efficiently eliminate mycelial growth and reduce the overwintering inoculum of their mycohosts. In addition, Ampelomyces isolates were found to be resistant to some fungicides and insecticides, e.g. pyrazophos, an attractive feature for their selection as biocontrol agents. Transcriptome analyses have revealed that expression of the genes that encode proteins putatively associated with virulence and plant immune responses were enhanced during host recognition, while genes-encoding proteins linked to antibiotic resistance were predicted within the Ampelomyces genome. Proteomic studies are needed to confirm whether these proteins function in virulence and can therefore be used for biocontrol purposes or as bacterial antibiotic-resistant proteins, and which of these may trigger plant immune responses to facilitate plant protection. We encourage the continuation of these studies to benefit crop protection research.","PeriodicalId":9468,"journal":{"name":"Canadian Journal of Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46794739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-10DOI: 10.1080/07060661.2023.2212639
K. Hollman, V. Manolii, Y. Aigu, M. Harding, S. Hwang, S. Strelkov
Abstract Clubroot, caused by Plasmodiophora brassicae, is a major soilborne disease of canola (Brassica napus) on the Canadian Prairies. Most canola cultivars carry what is now referred to as ‘first-generation’ resistance, which represents the most widely used clubroot management tool. Unfortunately, new pathotypes of P. brassicae have emerged that can overcome this resistance. By 2018, 36 unique pathotypes of P. brassicae had been identified on the Prairies based on their virulence on the Canadian Clubroot Differential (CCD) set, with pathotypes 3A, 3D and 3 H being predominant. Since the virulence of P. brassicae populations can shift rapidly in response to host selection pressure, continued pathotype monitoring is important for resistance stewardship. Clubbed roots were collected from 133 canola crops (126 in Alberta, 6 in Saskatchewan, 1 in Manitoba) in 2019 and 127 crops (113 in Alberta, 13 in Saskatchewan, 1 in Manitoba) in 2020, and evaluated for their CCD pathotype designations. Twenty-five unique pathotypes were identified across the sampled crops over the two years of the study; these included seven novel pathotypes, six of which could overcome first-generation resistance (3C, 8 G, 8I, 9D, 9E and 9F). While pathotypes 3A, 3D and 3 H continued to be identified frequently in 2019 and 2020, several others, most notably pathotype 8E (virulent on hosts with first-generation resistance), had also become more common by 2020. The results indicate the continued emergence of new P. brassicae pathotypes on canola.
{"title":"Characterization of Plasmodiophora brassicae pathotypes from western Canada in 2019-2020","authors":"K. Hollman, V. Manolii, Y. Aigu, M. Harding, S. Hwang, S. Strelkov","doi":"10.1080/07060661.2023.2212639","DOIUrl":"https://doi.org/10.1080/07060661.2023.2212639","url":null,"abstract":"Abstract Clubroot, caused by Plasmodiophora brassicae, is a major soilborne disease of canola (Brassica napus) on the Canadian Prairies. Most canola cultivars carry what is now referred to as ‘first-generation’ resistance, which represents the most widely used clubroot management tool. Unfortunately, new pathotypes of P. brassicae have emerged that can overcome this resistance. By 2018, 36 unique pathotypes of P. brassicae had been identified on the Prairies based on their virulence on the Canadian Clubroot Differential (CCD) set, with pathotypes 3A, 3D and 3 H being predominant. Since the virulence of P. brassicae populations can shift rapidly in response to host selection pressure, continued pathotype monitoring is important for resistance stewardship. Clubbed roots were collected from 133 canola crops (126 in Alberta, 6 in Saskatchewan, 1 in Manitoba) in 2019 and 127 crops (113 in Alberta, 13 in Saskatchewan, 1 in Manitoba) in 2020, and evaluated for their CCD pathotype designations. Twenty-five unique pathotypes were identified across the sampled crops over the two years of the study; these included seven novel pathotypes, six of which could overcome first-generation resistance (3C, 8 G, 8I, 9D, 9E and 9F). While pathotypes 3A, 3D and 3 H continued to be identified frequently in 2019 and 2020, several others, most notably pathotype 8E (virulent on hosts with first-generation resistance), had also become more common by 2020. The results indicate the continued emergence of new P. brassicae pathotypes on canola.","PeriodicalId":9468,"journal":{"name":"Canadian Journal of Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49131980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-04DOI: 10.1080/07060661.2023.2195251
R. Aboukhaddour, P. Lokuruge, A. Mulenga, G. Peng, W. May, R. Mohr, G. Telmosse, D. Pageau, A. Foster, B. Blackwell, H. Kubota
Pyrenophora tritici-repentis (tan spot) is a complex foliar pathogen that primarily infects hexaploid (bread) and tetraploid (durum) wheat, as well as a variety of wild grass species. Eight races of P. tritici-repentis have been described worldwide, based on their virulence patterns on a host differential set. The virulence of these races reflects their capacity to produce three known necrotrophic effectors (NE), either alone or in various combinations. Recently, ‘atypical’ isolates of P. tritici-repentis , which induce the necrosis typical of some of these races but lack the corresponding NE, were identified from durum wheat in North Africa (Tunisia), a secondary centre of diversity of this crop. The aim of this study was to char-acterize additional collections of P. tritici-repentis from Tunisia, in order to determine their virulence profiles and assist in the identification of novel races and NEs. Preliminary characterization of 53 isolates on the standard differential set indicated that 16.6% were classified as races 3 and 8, while 50% were race 5. In addition, 16.6% of the isolates appeared to induce atypical responses, meaning that the host response to inoculation did not seem to fit the standard reactions to the eight known races. Additional testing is underway to confirm these reactions, and all isolates will be assessed for the presence or absence of the known NE-encoding genes. An improved knowledge of the virulence of P. tritici-repentis , including the occurrence of novel races and NEs, will be important for the sustainable management
{"title":"Alberta regional meeting, 2022 / Réunion régionale de l’Alberta, 2022","authors":"R. Aboukhaddour, P. Lokuruge, A. Mulenga, G. Peng, W. May, R. Mohr, G. Telmosse, D. Pageau, A. Foster, B. Blackwell, H. Kubota","doi":"10.1080/07060661.2023.2195251","DOIUrl":"https://doi.org/10.1080/07060661.2023.2195251","url":null,"abstract":"Pyrenophora tritici-repentis (tan spot) is a complex foliar pathogen that primarily infects hexaploid (bread) and tetraploid (durum) wheat, as well as a variety of wild grass species. Eight races of P. tritici-repentis have been described worldwide, based on their virulence patterns on a host differential set. The virulence of these races reflects their capacity to produce three known necrotrophic effectors (NE), either alone or in various combinations. Recently, ‘atypical’ isolates of P. tritici-repentis , which induce the necrosis typical of some of these races but lack the corresponding NE, were identified from durum wheat in North Africa (Tunisia), a secondary centre of diversity of this crop. The aim of this study was to char-acterize additional collections of P. tritici-repentis from Tunisia, in order to determine their virulence profiles and assist in the identification of novel races and NEs. Preliminary characterization of 53 isolates on the standard differential set indicated that 16.6% were classified as races 3 and 8, while 50% were race 5. In addition, 16.6% of the isolates appeared to induce atypical responses, meaning that the host response to inoculation did not seem to fit the standard reactions to the eight known races. Additional testing is underway to confirm these reactions, and all isolates will be assessed for the presence or absence of the known NE-encoding genes. An improved knowledge of the virulence of P. tritici-repentis , including the occurrence of novel races and NEs, will be important for the sustainable management","PeriodicalId":9468,"journal":{"name":"Canadian Journal of Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45920052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-04DOI: 10.1080/07060661.2023.2202486
G., J., Bilodeau, C. Beaulieu
Emerging outbreaks of plant diseases pose enormous threat to agricultural production and global food security. Early detection and identification of plant pathogens using next-generation sequencing (NGS) technology and bioinformatics analysis are important to cope with the increase of international trade. Here, we present the PolyChrome bioinformatics toolkit for the detection and identification of regulated plant diseases. The PolyChrome toolkit con-sists of two programs, PolyChrome Detector (PCD) and PolyChrome Classifier (PCC). The former detects the presence of specific species from metagenomic and meta-transcriptomic data and the latter focus on the classification of closely related microorganisms at species or sub-species levels. In the PCD workflow, adapters and low-quality reads of raw NGS sequences are removed using Atria, an in-house designed trimming program. Clean reads are mapped to individual genomes, and then assembled to larger contigs, which are aligned to databases with taxonomy assignment. At the end of the pipeline, the annotated contigs are filtered with statistics on identity, alignment lengths, and bit scores, and suspected contigs of pathogens are reported. In PCC platform analysis, we first built curated PCC databases of selected regulated agents, e.g. Clavibacter, Liberibacter, Dickeya and Pectobacter , containing the genome sequences, anno-tations and the pre-analysis results, including average nucleotide identity (ANI) values. Testing dataset goes through the similar pipeline as PCD for contig generation and are classified using ANI values. The PolyChrome with PCD and PCC pipelines have been used to detect and identify plant pathogens, and has great potential in the detection of potato wart pathogen in soil.
{"title":"Annual meeting, the Canadian phytopathological society, 2022/Réunion annuelle, la société canadienne de phytopathologie, 2022","authors":"G., J., Bilodeau, C. Beaulieu","doi":"10.1080/07060661.2023.2202486","DOIUrl":"https://doi.org/10.1080/07060661.2023.2202486","url":null,"abstract":"Emerging outbreaks of plant diseases pose enormous threat to agricultural production and global food security. Early detection and identification of plant pathogens using next-generation sequencing (NGS) technology and bioinformatics analysis are important to cope with the increase of international trade. Here, we present the PolyChrome bioinformatics toolkit for the detection and identification of regulated plant diseases. The PolyChrome toolkit con-sists of two programs, PolyChrome Detector (PCD) and PolyChrome Classifier (PCC). The former detects the presence of specific species from metagenomic and meta-transcriptomic data and the latter focus on the classification of closely related microorganisms at species or sub-species levels. In the PCD workflow, adapters and low-quality reads of raw NGS sequences are removed using Atria, an in-house designed trimming program. Clean reads are mapped to individual genomes, and then assembled to larger contigs, which are aligned to databases with taxonomy assignment. At the end of the pipeline, the annotated contigs are filtered with statistics on identity, alignment lengths, and bit scores, and suspected contigs of pathogens are reported. In PCC platform analysis, we first built curated PCC databases of selected regulated agents, e.g. Clavibacter, Liberibacter, Dickeya and Pectobacter , containing the genome sequences, anno-tations and the pre-analysis results, including average nucleotide identity (ANI) values. Testing dataset goes through the similar pipeline as PCD for contig generation and are classified using ANI values. The PolyChrome with PCD and PCC pipelines have been used to detect and identify plant pathogens, and has great potential in the detection of potato wart pathogen in soil.","PeriodicalId":9468,"journal":{"name":"Canadian Journal of Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47953057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-04DOI: 10.1080/07060661.2023.2195254
Determining the genotypes of Plasmodiophora brassicae populations collected in Western Canada and developing SNP markers for the P. brassicae races
测定加拿大西部采集的芸苔疟原虫群体的基因型并开发芸苔疟原虫小种的SNP标记
{"title":"Saskatchewan regional meeting, 2022 / Réunion régionale de la Saskatchewan, 2022","authors":"","doi":"10.1080/07060661.2023.2195254","DOIUrl":"https://doi.org/10.1080/07060661.2023.2195254","url":null,"abstract":"Determining the genotypes of Plasmodiophora brassicae populations collected in Western Canada and developing SNP markers for the P. brassicae races","PeriodicalId":9468,"journal":{"name":"Canadian Journal of Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45785090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-27DOI: 10.1080/07060661.2023.2208088
Jiaxi Li, An Zhang, Yan Liu, Yaowei Zhang, Ran Gu
Abstract A new kind of postharvest rot on Chinese cabbage (Brassica rapa L. ssp. pekinensis) caused by a fungus was observed in vegetable cellars in Harbin, China in 2019 and 2020, causing considerable economic losses. A water-soaked spot appeared at the base of the midrib of leaves, extended into the upper part of the midrib, and ultimately turned into rot. The diseased part was dark brown or black with a few black spores on the surface. Fungal isolates were obtained from the diseased plants and identified as Aspergillus welwitschiae through morphological observation and multigene sequencing analysis of the internal transcribed spacer, β-tubulin and calmodulin genetic regions. Pathogenicity tests were conducted, and the resulting symptoms on Chinese cabbage were similar to those seen in the vegetable cellar. The isolates were also associated with rot and leaf spot on seedlings and affected the seed germination of Chinese cabbage. A host range test showed that the isolates could infect some common vegetables, including carrot, kidney bean, broccoli, radish, Chinese flowering cabbage, root-mustard, cabbage, non-heading Chinese cabbage, pepper, lettuce, oilseed rape, tomato and cucumber. The isolation, identification, and host range of the pathogen can provide a basis for the study of the occurrence, prevention and management of the disease in the future.
{"title":"Isolation, identification, and host range of Aspergillus welwitschiae causing postharvest rot on Chinese cabbage in China","authors":"Jiaxi Li, An Zhang, Yan Liu, Yaowei Zhang, Ran Gu","doi":"10.1080/07060661.2023.2208088","DOIUrl":"https://doi.org/10.1080/07060661.2023.2208088","url":null,"abstract":"Abstract A new kind of postharvest rot on Chinese cabbage (Brassica rapa L. ssp. pekinensis) caused by a fungus was observed in vegetable cellars in Harbin, China in 2019 and 2020, causing considerable economic losses. A water-soaked spot appeared at the base of the midrib of leaves, extended into the upper part of the midrib, and ultimately turned into rot. The diseased part was dark brown or black with a few black spores on the surface. Fungal isolates were obtained from the diseased plants and identified as Aspergillus welwitschiae through morphological observation and multigene sequencing analysis of the internal transcribed spacer, β-tubulin and calmodulin genetic regions. Pathogenicity tests were conducted, and the resulting symptoms on Chinese cabbage were similar to those seen in the vegetable cellar. The isolates were also associated with rot and leaf spot on seedlings and affected the seed germination of Chinese cabbage. A host range test showed that the isolates could infect some common vegetables, including carrot, kidney bean, broccoli, radish, Chinese flowering cabbage, root-mustard, cabbage, non-heading Chinese cabbage, pepper, lettuce, oilseed rape, tomato and cucumber. The isolation, identification, and host range of the pathogen can provide a basis for the study of the occurrence, prevention and management of the disease in the future.","PeriodicalId":9468,"journal":{"name":"Canadian Journal of Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48369924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-25DOI: 10.1080/07060661.2023.2202481
{"title":"In memoriam / En mémoire: Dr. Susan M. Boyetchko January 4, 1959–February 8, 2023","authors":"","doi":"10.1080/07060661.2023.2202481","DOIUrl":"https://doi.org/10.1080/07060661.2023.2202481","url":null,"abstract":"","PeriodicalId":9468,"journal":{"name":"Canadian Journal of Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46839424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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