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Functional assessment of MeCP2 in Rett syndrome and cancers of breast, colon, and prostate. MeCP2在Rett综合征和乳腺癌、结肠癌、前列腺癌中的功能评估。
Somnath Pandey, Kevin Pruitt
Ever since the first report that mutations in methyl-CpG-binding protein 2 (MeCP2) causes Rett syndrome (RTT), a severe neurological disorder in females world-wide, there has been a keen interest to gain a comprehensive understanding of this protein. While the classical model associated with MeCP2 function suggests its role in gene suppression via recruitment of co-repressor complexes and histone deacetylases to methylated CpG-sites, recent discoveries have brought to light its role in transcription activation, modulation of RNA splicing, and chromatin compaction. Various post-translational modifications (PTMs) of MeCP2 further increase its functional versatility. Involvement of MeCP2 in pathologies other than RTT, such as tumorigenesis however, remains poorly explored and understood. This review provides a survey of the literature implicating MeCP2 in breast, colon and prostate cancer.
自从首次报道甲基cpg结合蛋白2 (MeCP2)突变导致Rett综合征(RTT)以来,人们一直对该蛋白有浓厚的兴趣,以获得对该蛋白的全面了解。Rett综合征是一种严重的女性神经系统疾病。虽然与MeCP2功能相关的经典模型表明,它通过向甲基化的cpg位点募集共阻遏物复合物和组蛋白去乙酰化酶来抑制基因,但最近的发现揭示了它在转录激活、RNA剪接调节和染色质压实中的作用。MeCP2的各种翻译后修饰(PTMs)进一步增加了其功能的通用性。然而,MeCP2参与RTT以外的病理,如肿瘤发生,仍然缺乏探索和了解。本文综述了MeCP2与乳腺癌、结肠癌和前列腺癌相关的文献。
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引用次数: 12
Potential regulatory mechanisms of lncRNA in diabetes and its complications. lncRNA在糖尿病及其并发症中的潜在调控机制。
Shuidong Feng, Ji-Hua Yang, C. Yao, Sisi Yang, Ze-Mei Zhu, Di Wu, H. Ling, Liang Zhang
Long non-coding RNAs (lncRNAs) are transcripts longer than 200 nucleotides without protein-coding potential. Although these molecules were initially considered as "junk products" of transcription without biological relevance, recent advances in research have shown that lncRNA plays an important role, not only in cellular processes such as proliferation, differentiation, and metabolism, but also in the pathological processes of cancers, diabetes, and neurodegenerative diseases. In this review, we focus on the potential regulatory roles of lncRNA in diabetes and the complications associated with diabetes.
长链非编码rna (lncRNAs)是长度超过200个核苷酸的转录本,没有蛋白质编码潜力。虽然这些分子最初被认为是转录的“垃圾产品”,没有生物学相关性,但最近的研究进展表明,lncRNA不仅在细胞增殖、分化和代谢等过程中发挥重要作用,而且在癌症、糖尿病和神经退行性疾病的病理过程中也发挥着重要作用。在这篇综述中,我们重点关注lncRNA在糖尿病和糖尿病相关并发症中的潜在调节作用。
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引用次数: 60
Chronic intermittent hypoxia disturbs insulin secretion and causes pancreatic injury via the MAPK signaling pathway. 慢性间歇性缺氧通过MAPK信号通路干扰胰岛素分泌,导致胰腺损伤。
Yeying Wang, B. Hai, Xiaoqun Niu, Li Ai, Yu Cao, Ran Li, Yongxia Li
Obstructive sleep apnea (OSA) is a breathing disorder during sleep, with a most prominent character of chronic intermittent hypoxia (CIH), which induces the generation of reactive oxygen species (ROS) that damages multiple tissues and causes metabolic disorders. In this study, we established a rat model of varying OSA with different grades of CIH (12.5% O2, 10% O2, 7.5% O2, and 5% O2) for 12 weeks, and found that CIH stimulated insulin secretion, reduced the insulin:proinsulin ratio in pancreatic tissue, and caused pancreatic tissue lesions and cell apoptosis in a dose-dependent manner. Moreover, CIH promoted the production of tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6, and activated mitogen-activated protein kinase (MAPK) family members, extracellular regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), and P38, depending on the O2 concentration. In summary, CIH disturbed insulin secretion, and caused inflammation, lesions, and cell apoptosis in pancreatic tissue via the MAPK signaling pathway, which may be of great significance for clinical treatment of OSA and type 2 diabetes mellitus (T2DM).
阻塞性睡眠呼吸暂停(OSA)是一种睡眠中的呼吸障碍,其最突出的特征是慢性间歇性缺氧(CIH),它诱导活性氧(ROS)的产生,损害多组织并引起代谢紊乱。本研究建立不同浓度CIH (12.5% O2、10% O2、7.5% O2、5% O2)持续12周的OSA大鼠模型,发现CIH刺激胰岛素分泌,降低胰腺组织胰岛素:胰岛素原比值,引起胰腺组织病变和细胞凋亡呈剂量依赖性。此外,CIH促进肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β和IL-6的产生,并根据O2浓度激活丝裂原活化蛋白激酶(MAPK)家族成员、细胞外调节蛋白激酶(ERK)、c-Jun n-末端激酶(JNK)和P38。综上所述,CIH通过MAPK信号通路干扰胰腺组织胰岛素分泌,引起胰腺组织炎症、病变和细胞凋亡,对OSA和2型糖尿病(T2DM)的临床治疗可能具有重要意义。
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引用次数: 27
Linker histones: novel insights into structure-specific recognition of the nucleosome. 连接体组蛋白:核小体结构特异性识别的新见解。
Amber R. Cutter, J. Hayes
Linker histones (H1s) are a primary component of metazoan chromatin, fulfilling numerous functions, both in vitro and in vivo, including stabilizing the wrapping of DNA around the nucleosome, promoting folding and assembly of higher order chromatin structures, influencing nucleosome spacing on DNA, and regulating specific gene expression. However, many molecular details of how H1 binds to nucleosomes and recognizes unique structural features on the nucleosome surface remain undefined. Numerous, confounding studies are complicated not only by experimental limitations, but the use of different linker histone isoforms and nucleosome constructions. This review summarizes the decades of research that has resulted in several models of H1 association with nucleosomes, with a focus on recent advances that suggest multiple modes of H1 interaction in chromatin, while highlighting the remaining questions.
连接体组蛋白(h1)是后生动物染色质的主要组成部分,在体外和体内都具有多种功能,包括稳定DNA在核小体周围的包裹,促进高阶染色质结构的折叠和组装,影响DNA上的核小体间距,以及调节特定基因的表达。然而,H1如何与核小体结合并识别核小体表面独特结构特征的许多分子细节仍不清楚。许多混杂的研究不仅由于实验限制,而且由于使用了不同的连接蛋白异构体和核小体结构而变得复杂。这篇综述总结了几十年来的研究结果,得出了H1与核小体相关的几种模型,重点介绍了H1在染色质中相互作用的多种模式的最新进展,同时强调了剩余的问题。
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引用次数: 20
MgF3- and AlF4- transition state analogue complexes of yeast phosphoglycerate kinase. 酵母磷酸甘油酸激酶的过渡态类似物MgF3-和AlF4。
N. McCormick, S. Forget, R. Syvitski, D. Jakeman
The phospho-transfer mechanism of yeast phosphoglycerate kinase (PGK) has been probed through formation of trifluoromagnesate (MgF3-) and tetrafluoroaluminate (AlF4-) transition state analogue complexes and analyzed using 19F, 1H waterLOGSY and 1H chemical shift perturbation NMR spectroscopy. We observed the first 19F NMR spectroscopic evidence for the formation of metal fluoride transition state analogues of yeast PGK and also observed significant changes to proton chemical shifts of PGK in the presence, but not in the absence, of fluoride upon titration of ligands, providing indirect evidence of the formation of a closed ternary transition state. WaterLOGSY NMR spectroscopy experiments using an uncompetitive model were used in an attempt to measure ligand binding affinities within the transition state analogue complexes.
通过三氟镁酸盐(MgF3-)和四氟铝酸盐(AlF4-)过渡态类似物的形成,探讨了酵母磷酸甘油酸激酶(PGK)的磷酸转移机制,并利用19F、1H waterLOGSY和1H化学位移摄动NMR谱分析了PGK的磷酸转移机制。我们观察到第一个19F核磁共振光谱证据,证明了酵母PGK的金属氟化物过渡态类似物的形成,并观察到在配体滴定时,PGK的质子化学位移在有氟的情况下(而不是没有氟的情况下)发生了显著变化,为形成封闭的三联过渡态提供了间接证据。使用非竞争模型的WaterLOGSY核磁共振波谱实验被用于测量过渡态类似物内的配体结合亲和力。
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引用次数: 2
Vitamin D3 protects against prednisolone-induced liver injury associated with the impairment of the hepatic NF-κB/iNOS/NO pathway. 维生素D3对强的松龙诱导的肝损伤具有保护作用,肝损伤与肝NF-κB/iNOS/NO通路的损害有关。
O. Lisakovska, I. Shymanskyy, A. Mazanova, A. Khomenko, M. Veliky
The study was carried out to define whether prednisolone-induced damage to hepatic cells is accompanied by excessive nitric oxide (NO) levels associated with nuclear factor kappa B (NF-κB)/inducible NO synthase (iNOS) activation and evaluate the efficacy of the treatment with vitamin D3. Histopathological examination, activities of liver transaminases (alanine aminotransferase and aspartate aminotransferase), and cell death assays consistently showed that prednisolone (5 mg/kg body weight, 30 days) induces chronic liver injury in female Wistar rats. Specifically, increased hepatocellular necrosis and caspase-3-dependent apoptosis were observed. Prednisolone enhanced iNOS protein expression, NO generation, and tyrosine nitration in liver cells. Despite unchanged hepatic level of the NF-κB/p65 protein, prednisolone increased inhibitory κB-α (IκB-α) degradation, nuclear translocation, and phosphorylation of NF-κB/p65 at Ser311, indicating that NF-κB activation can be involved in the induction of iNOS/NO. All changes were associated with a 2.9-fold decrease in the serum content of 25-hydroxyvitamin D3 and significant reduction of hepatic vitamin D3 receptor (VDR) expression that points reliably to vitamin D3 deficiency and failures in VDR signaling. Vitamin D3 co-administration (100 IU/rat, 30 days) prevented glucocorticoid-evoked abnormalities in hepatic tissue. In conclusion, prednisolone-induced liver disturbances were associated with the impairment of NF-κB/iNOS/NO responses that can be ameliorated by vitamin D3 treatment through VDR-mediated mechanisms.
本研究旨在确定强的松龙诱导的肝细胞损伤是否伴有与核因子κB (NF-κB)/诱导型NO合成酶(iNOS)激活相关的过量一氧化氮(NO)水平,并评估维生素D3治疗的疗效。组织病理学检查、肝转氨酶(丙氨酸转氨酶和天冬氨酸转氨酶)活性和细胞死亡实验一致表明,强的松龙(5 mg/kg体重,30天)诱导雌性Wistar大鼠慢性肝损伤。具体而言,观察到肝细胞坏死和caspase-3依赖性凋亡增加。强的松龙增强肝细胞iNOS蛋白表达、NO生成和酪氨酸硝化。尽管肝脏中NF-κB/p65蛋白水平不变,但强的松龙增加了抑制κB-α (i -κB -α)降解、核易位和NF-κB/p65在311位点的磷酸化,表明NF-κB活化可能参与了iNOS/NO的诱导。所有这些变化都与血清25-羟基维生素D3含量降低2.9倍和肝脏维生素D3受体(VDR)表达显著降低有关,这可靠地表明维生素D3缺乏和VDR信号传导失败。维生素D3联合给药(100 IU/大鼠,30天)可防止糖皮质激素引起的肝组织异常。综上所述,强的松龙引起的肝脏紊乱与NF-κB/iNOS/NO反应的损害有关,而维生素D3治疗可以通过vdr介导的机制改善这种损害。
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引用次数: 21
Ethanol-dependent expression of the NKG2D ligands MICA/B in human cell lines and leukocytes. NKG2D配体MICA/B在人细胞系和白细胞中的乙醇依赖性表达。
M. Streltsova, A. V. Klinkova, Anastasia A Kuchukova, A. Y. Kadin, L. Kanevskiy, E. Kovalenko
Alcohol consumption affects the human immune system, causing a variety of disorders. However, the mechanisms of development of these changes are not fully understood. We hypothesized that ethanol may influence the expression of MICA and MICB, stress-induced molecules capable of regulating the activity of cytotoxic lymphocytes through the interaction with receptor NKG2D, which substantially affects the functionality of cellular immunity. We analyzed the effects of ethanol on MICA/B expression in tumor cell lines and human leukocytes. In the cell line models, ethanol caused different changes in the surface expression of MICA/B; in particular, it induced the translocation of intracellular proteins MICA/B to the cell surface and shedding of MICA (in soluble and microparticle-associated forms) from the plasma membrane. The observed results are not linked with cell death in cultures, taking place only under higher doses of ethanol. Ethanol at physiologically relevant concentrations (and higher) stimulated expression of MICA/B genes in different cell types. The effect of ethanol was more pronounced in hepatocyte line HepG2 compared with hematopoietic cell lines K562, Jurkat, and THP-1. Among the tested leukocytes, the most sensitive to ethanol action were T cells activated ex vivo with IL-2, in which the increase of MICA/B mRNA expression was registered with the smallest dose of ethanol (0.125%). In human monocytes, ethanol may lead to elevations in surface MICA/B levels. Presumably, changes in MICA/B expression caused by ethanol can affect the functions of NKG2D-positive cytotoxic lymphocytes, modulating immune reactions at excessive alcohol consumption.
饮酒会影响人体免疫系统,导致各种疾病。然而,这些变化的发展机制尚不完全清楚。我们假设乙醇可能影响MICA和MICB的表达,MICA和MICB是能够通过与受体NKG2D相互作用调节细胞毒性淋巴细胞活性的应激诱导分子,从而实质性地影响细胞免疫功能。我们分析了乙醇对肿瘤细胞系和人白细胞MICA/B表达的影响。在细胞系模型中,乙醇引起MICA/B表面表达的不同变化;特别是,它诱导细胞内蛋白MICA/B易位到细胞表面,并从质膜上脱落MICA(以可溶性和微粒相关形式)。观察到的结果与培养中的细胞死亡无关,仅在较高剂量的乙醇下发生。生理相关浓度(或更高)的乙醇刺激MICA/B基因在不同细胞类型中的表达。与造血细胞系K562、Jurkat和THP-1相比,乙醇对肝细胞系HepG2的影响更为明显。在被检测的白细胞中,对乙醇作用最敏感的是体外被IL-2激活的T细胞,其中MICA/B mRNA的表达在最小剂量的乙醇(0.125%)下增加。在人单核细胞中,乙醇可导致表面MICA/B水平升高。据推测,乙醇引起的MICA/B表达变化可影响nkg2d阳性细胞毒性淋巴细胞的功能,调节过量饮酒时的免疫反应。
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引用次数: 1
Cellular stress associated with the differentiation of Plasmodium berghei ookinetes. 细胞应激与伯氏疟原虫的分化有关。
Josefina Duran-Bedolla, J. Téllez-Sosa, H. Valdovinos-Torres, N. Pavón, M. Buelna-Chontal, Ángel T. Tello-López, R. Argotte-Ramos, Mario H. Rodríguez, M. C. Rodríguez
For malaria transmission, Plasmodium parasites must develop in the mosquito vector. Oxidative stress in the insect midgut, triggered by environmental changes (e.g., pH and temperature), influences the cellular signaling involved in differentiation from gametocytes to mobile ookinetes for the purpose of parasite survival. Oxidative stress activates the homeostatic response to stress characterized by the phosphorylation eIF2α, the attenuation of protein synthesis, and the transcription of genes participating in the unfolded protein response and antioxidant processes, forming a part of an integrated stress response (ISR). We hypothesized that ISR operates during the differentiation of gametocytes to ookinetes to assure Plasmodium survival. Using in-vitro conditions resembling the mosquito midgut conditions, we cultured Plasmodium berghei gametocytes to ookinetes and evaluated the redox balance by detecting reactive oxygen species and superoxide dismutase activity. Additionally, we evaluated the phosphorylation of eIF2α, the attenuation of the global protein synthesis, and the gene expression of cellular stress markers (e.g., endoplasmic reticulum chaperones and antioxidant molecules, measured by reverse-transcription quantitative polymerase chain reaction), finding that these processes were all taking place, probably to improve survival during the differentiation of Plasmodium berghei ookinetes.
对于疟疾传播,疟原虫必须在蚊子载体中发育。由环境变化(如pH值和温度)引发的昆虫中肠氧化应激影响了从配子体向活动卵母细胞分化的细胞信号传导,从而使寄生虫存活。氧化应激激活以eIF2α磷酸化、蛋白合成衰减以及参与未折叠蛋白反应和抗氧化过程的基因转录为特征的应激稳态反应,构成综合应激反应(ISR)的一部分。我们假设ISR在配子细胞向单胞细胞分化的过程中起作用,以确保疟原虫的存活。在类似于蚊子中肠的体外条件下,我们将伯氏疟原虫配子体培养成单核细胞,并通过检测活性氧和超氧化物歧化酶活性来评估氧化还原平衡。此外,我们评估了eIF2α的磷酸化,全球蛋白合成的衰减,以及细胞应激标志物(如内质网伴侣和抗氧化分子,通过逆转录定量聚合酶链反应测量)的基因表达,发现这些过程都在发生,可能是为了提高伯氏疟原虫在分化过程中的存活率。
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引用次数: 8
Proteomics analysis of latex from Hevea brasiliensis (clone RRIM 600). 巴西橡胶树(克隆RRIM 600)乳胶的蛋白质组学分析。
Mohd Afiq Hazlami Habib, Gan Chee Yuen, Fazilah Othman, Nurul Nabilah Zainudin, A. Latiff, M. Ismail
The natural rubber latex extracted from the bark of Hevea brasiliensis plays various important roles in today's modern society. Following ultracentrifugation, the latex can be separated into 3 layers: C-serum, lutoids, and rubber particles. Previous studies have shown that a large number of proteins are present in these 3 layers. However, a complete proteome for this important plant is still unavailable. Protein sequences have been recently translated from the completed draft genome database of H. brasiliensis, leading to the creation of annotated protein databases of the following H. brasiliensis biosynthetic pathways: photosynthesis, latex allergens, rubberwood formation, latex biosynthesis, and disease resistance. This research was conducted to identify the proteins contained within the latex by way of de novo sequencing from mass spectral data obtained from the 3 layers of the latex. Peptides from these proteins were fragmented using collision-induced dissociation, higher-energy collisional dissociation, and electron-transfer dissociation activation methods. A large percentage of proteins from the biosynthetic pathways (63% to 100%) were successfully identified. In addition, a total of 1839 unique proteins were identified from the whole translated draft genome database (AnnHBM).
从巴西橡胶树树皮中提取的天然胶乳在当今现代社会中发挥着各种重要作用。经过超离心处理,乳胶可分为三层:c -血清、类鲁特体和橡胶颗粒。先前的研究表明,在这3层中存在大量的蛋白质。然而,这种重要植物的完整蛋白质组仍然不可用。最近,从巴西橡胶树已完成的基因组草图数据库中翻译了蛋白质序列,从而建立了巴西橡胶树生物合成途径的注释蛋白质数据库:光合作用、乳胶过敏原、橡胶木形成、乳胶生物合成和抗病。本研究利用从三层乳胶中获得的质谱数据,采用从头测序的方法鉴定乳胶中所含的蛋白质。通过碰撞诱导解离、高能碰撞解离和电子转移解离激活等方法,将这些蛋白质的肽片段化。从生物合成途径中成功鉴定了很大比例的蛋白质(63%至100%)。此外,从整个翻译草图基因组数据库(AnnHBM)中共鉴定出1839个独特的蛋白质。
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引用次数: 13
Adaptations to excess choline in insulin resistant and Pcyt2 deficient skeletal muscle. 胰岛素抵抗和Pcyt2缺乏骨骼肌对过量胆碱的适应。
Adrian Taylor, L. Schenkel, Maiya K. Yokich, M. Bakovic
It was hypothesized that choline supplementation in insulin resistant (IR) CTP:phosphoethanolamine cytidylyltransferase deficient (Pcyt2+/-) mice would ameliorate muscle function by remodeling glucose and fatty acid (FA) metabolism. Pcyt2+/- mice either received no treatment or were allowed access to 2 mg/mL choline in drinking water for 4 weeks. Skeletal muscle was harvested from choline treated and untreated mice. Lipid analysis and metabolic gene expression and signaling pathways were compared between untreated Pcyt2+/- mice, treated Pcyt2+/- mice, and Pcyt2+/+ mice. The major positive effect of choline supplementation on IR muscle was the reduction of glucose utilization for FA and triglyceride (TAG) synthesis and increased muscle glucose storage as glycogen. Choline reduced the expression of genes for FA and TAG formation (Scd1, Fas, Srebp1c, Dgat1/2), upregulated the genes for FA oxidation (Cpt1, Pparα, Pgc1α), and had minor effects on phospholipid and lipolysis genes. Pcyt2+/- muscle had reduced insulin signaling (IRS1), autophagy (LC3), and choline transport (CTL1) proteins that were restored by choline treatment. Additionally, choline activated AMPK and Akt while inhibiting mTORC1 phosphorylation. These data established that choline supplementation could restore muscle glucose metabolism by reducing lipogenesis and improving mitochondrial and intracellular signaling for protein and energy metabolism in insulin resistant Pcyt2 deficient mice.
据推测,在胰岛素抵抗(IR) CTP:磷酸乙醇胺胞基转移酶缺陷(Pcyt2+/-)小鼠中补充胆碱可以通过重塑葡萄糖和脂肪酸(FA)代谢来改善肌肉功能。Pcyt2+/-小鼠要么不接受治疗,要么连续4周在饮用水中加入2 mg/mL胆碱。从胆碱处理和未处理的小鼠身上采集骨骼肌。比较Pcyt2+/-小鼠、Pcyt2+/-小鼠和Pcyt2+/+小鼠的脂质分析、代谢基因表达和信号通路。补充胆碱对IR肌肉的主要积极影响是减少了FA和甘油三酯(TAG)合成的葡萄糖利用,增加了作为糖原的肌肉葡萄糖储存。胆碱降低了FA和TAG形成基因(Scd1, Fas, Srebp1c, Dgat1/2)的表达,上调了FA氧化基因(Cpt1, Pparα, Pgc1α)的表达,对磷脂和脂肪分解基因的影响较小。Pcyt2+/-肌肉的胰岛素信号(IRS1)、自噬(LC3)和胆碱运输(CTL1)蛋白减少,这些蛋白通过胆碱处理得到恢复。此外,胆碱激活AMPK和Akt,抑制mTORC1磷酸化。这些数据表明,在胰岛素抵抗Pcyt2缺陷小鼠中,补充胆碱可以通过减少脂肪生成和改善线粒体和细胞内蛋白质和能量代谢的信号传导来恢复肌肉葡萄糖代谢。
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引用次数: 10
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Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire
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