Pub Date : 2025-02-13DOI: 10.1016/j.jcjo.2025.01.017
Michele Zaman, Davin Johnson
{"title":"Periocular humidity with different forms of face masking.","authors":"Michele Zaman, Davin Johnson","doi":"10.1016/j.jcjo.2025.01.017","DOIUrl":"https://doi.org/10.1016/j.jcjo.2025.01.017","url":null,"abstract":"","PeriodicalId":9606,"journal":{"name":"Canadian journal of ophthalmology. Journal canadien d'ophtalmologie","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143432577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-11DOI: 10.1016/j.jcjo.2025.01.011
Fanghan Anna Yang, Leanne Stunkel, Manu S Goyal, Gregory P Van Stavern
{"title":"Mixed vascular lesion presenting as isolated vertical diplopia.","authors":"Fanghan Anna Yang, Leanne Stunkel, Manu S Goyal, Gregory P Van Stavern","doi":"10.1016/j.jcjo.2025.01.011","DOIUrl":"10.1016/j.jcjo.2025.01.011","url":null,"abstract":"","PeriodicalId":9606,"journal":{"name":"Canadian journal of ophthalmology. Journal canadien d'ophtalmologie","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143051676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-06DOI: 10.1016/j.jcjo.2025.01.003
Rodolfo Bonatti, Kevin Hodgson, Marcelo Nicolela
{"title":"Successful resolution of early fibrin occlusion of PreserFlo MicroShunt with Nd:YAG laser treatment.","authors":"Rodolfo Bonatti, Kevin Hodgson, Marcelo Nicolela","doi":"10.1016/j.jcjo.2025.01.003","DOIUrl":"10.1016/j.jcjo.2025.01.003","url":null,"abstract":"","PeriodicalId":9606,"journal":{"name":"Canadian journal of ophthalmology. Journal canadien d'ophtalmologie","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143051681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-05DOI: 10.1016/j.jcjo.2025.01.004
Haaris M Khan, Steven Schendel, Frederick S Mikelberg, Mahyar Etminan
{"title":"Acute angle-closure glaucoma risk: a comparative study of escitalopram and antidepressants.","authors":"Haaris M Khan, Steven Schendel, Frederick S Mikelberg, Mahyar Etminan","doi":"10.1016/j.jcjo.2025.01.004","DOIUrl":"10.1016/j.jcjo.2025.01.004","url":null,"abstract":"","PeriodicalId":9606,"journal":{"name":"Canadian journal of ophthalmology. Journal canadien d'ophtalmologie","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143051667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-05DOI: 10.1016/j.jcjo.2024.12.006
Alaa Tayyib, Deepika C Parameswarappa, Peter J Kertes, Rajeev Muni, Anupreet Tumber, Gregory Costain, Alex Schramm, Heather MacDonald, Regan Klatt, Ajoy Vincent, Elise Héon
Objective: Assess safety and effectiveness of subretinal gene replacement therapy at 18 months post treatment.
Design: Retrospective, longitudinal study conducted at the Hospital for Sick Children in Toronto, Canada.
Participants: Patients with bi-allelic RPE65 variants, early onset retinal degeneration, and residual viable retina who underwent voretigene neparvovec r-zyl gene replacement therapy.
Methods: Data collected included demographic information, molecular genetic results, and comprehensive ocular assessment results from preoperataive and postoperative visits up to 18 months. To assess the treatment's efficacy, postoperative best corrected visual acuity, full-field stimulus test (FST), visual field (VF) area, optical coherence tomography, and global satisfaction were compared to preoperative findings.
Results: The procedures were safe with no complications. There was no significant improvement in visual acuity. Three eyes showed a slight reduction in VF area. All showed a reduction in ellipsoid zone thickness and area, but the outer nuclear layer thickness and area were stable. All had a significant improvement in retinal sensitivity, as per FST, allowing better navigation in a dim environment. All 3 patients reported being "very satisfied".
Conclusion: Following a safe gene replacement therapy, the 3 first Canadian cases had an improvement in retinal sensitivity as per FST, and our patients described their experience as positively life changing.
{"title":"Insights into the effects of subretinal voretigene neparvovec-rzyl in RPE65-associated Leber congenital amaurosis.","authors":"Alaa Tayyib, Deepika C Parameswarappa, Peter J Kertes, Rajeev Muni, Anupreet Tumber, Gregory Costain, Alex Schramm, Heather MacDonald, Regan Klatt, Ajoy Vincent, Elise Héon","doi":"10.1016/j.jcjo.2024.12.006","DOIUrl":"10.1016/j.jcjo.2024.12.006","url":null,"abstract":"<p><strong>Objective: </strong>Assess safety and effectiveness of subretinal gene replacement therapy at 18 months post treatment.</p><p><strong>Design: </strong>Retrospective, longitudinal study conducted at the Hospital for Sick Children in Toronto, Canada.</p><p><strong>Participants: </strong>Patients with bi-allelic RPE65 variants, early onset retinal degeneration, and residual viable retina who underwent voretigene neparvovec r-zyl gene replacement therapy.</p><p><strong>Methods: </strong>Data collected included demographic information, molecular genetic results, and comprehensive ocular assessment results from preoperataive and postoperative visits up to 18 months. To assess the treatment's efficacy, postoperative best corrected visual acuity, full-field stimulus test (FST), visual field (VF) area, optical coherence tomography, and global satisfaction were compared to preoperative findings.</p><p><strong>Results: </strong>The procedures were safe with no complications. There was no significant improvement in visual acuity. Three eyes showed a slight reduction in VF area. All showed a reduction in ellipsoid zone thickness and area, but the outer nuclear layer thickness and area were stable. All had a significant improvement in retinal sensitivity, as per FST, allowing better navigation in a dim environment. All 3 patients reported being \"very satisfied\".</p><p><strong>Conclusion: </strong>Following a safe gene replacement therapy, the 3 first Canadian cases had an improvement in retinal sensitivity as per FST, and our patients described their experience as positively life changing.</p>","PeriodicalId":9606,"journal":{"name":"Canadian journal of ophthalmology. Journal canadien d'ophtalmologie","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-04DOI: 10.1016/j.jcjo.2025.01.001
Meziane Silhadi, Wissam B Nassrallah, David Mikhail, Daniel Milad, Mona Harissi-Dagher
Objective: To evaluate the performance of large language models (LLMs), specifically Microsoft Copilot, GPT-4 (GPT-4o and GPT-4o mini), and Google Gemini (Gemini and Gemini Advanced), in answering ophthalmological questions and assessing the impact of prompting techniques on their accuracy.
Design: Prospective qualitative study.
Participants: Microsoft Copilot, GPT-4 (GPT-4o and GPT-4o mini), and Google Gemini (Gemini and Gemini Advanced).
Methods: A total of 300 ophthalmological questions from StatPearls were tested, covering a range of subspecialties and image-based tasks. Each question was evaluated using 2 prompting techniques: zero-shot forced prompting (prompt 1) and combined role-based and zero-shot plan-and-solve+ prompting (prompt 2).
Results: With zero-shot forced prompting, GPT-4o demonstrated significantly superior overall performance, correctly answering 72.3% of questions and outperforming all other models, including Copilot (53.7%), GPT-4o mini (62.0%), Gemini (54.3%), and Gemini Advanced (62.0%) (p < 0.0001). Both Copilot and GPT-4o showed notable improvements with Prompt 2 over Prompt 1, elevating Copilot's accuracy from the lowest (53.7%) to the second highest (72.3%) among the evaluated LLMs.
Conclusions: While newer iterations of LLMs, such as GPT-4o and Gemini Advanced, outperformed their less advanced counterparts (GPT-4o mini and Gemini), this study emphasizes the need for caution in clinical applications of these models. The choice of prompting techniques significantly influences performance, highlighting the necessity for further research to refine LLMs capabilities, particularly in visual data interpretation, to ensure their safe integration into medical practice.
{"title":"Assessing the performance of Microsoft Copilot, GPT-4 and Google Gemini in ophthalmology.","authors":"Meziane Silhadi, Wissam B Nassrallah, David Mikhail, Daniel Milad, Mona Harissi-Dagher","doi":"10.1016/j.jcjo.2025.01.001","DOIUrl":"10.1016/j.jcjo.2025.01.001","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the performance of large language models (LLMs), specifically Microsoft Copilot, GPT-4 (GPT-4o and GPT-4o mini), and Google Gemini (Gemini and Gemini Advanced), in answering ophthalmological questions and assessing the impact of prompting techniques on their accuracy.</p><p><strong>Design: </strong>Prospective qualitative study.</p><p><strong>Participants: </strong>Microsoft Copilot, GPT-4 (GPT-4o and GPT-4o mini), and Google Gemini (Gemini and Gemini Advanced).</p><p><strong>Methods: </strong>A total of 300 ophthalmological questions from StatPearls were tested, covering a range of subspecialties and image-based tasks. Each question was evaluated using 2 prompting techniques: zero-shot forced prompting (prompt 1) and combined role-based and zero-shot plan-and-solve+ prompting (prompt 2).</p><p><strong>Results: </strong>With zero-shot forced prompting, GPT-4o demonstrated significantly superior overall performance, correctly answering 72.3% of questions and outperforming all other models, including Copilot (53.7%), GPT-4o mini (62.0%), Gemini (54.3%), and Gemini Advanced (62.0%) (p < 0.0001). Both Copilot and GPT-4o showed notable improvements with Prompt 2 over Prompt 1, elevating Copilot's accuracy from the lowest (53.7%) to the second highest (72.3%) among the evaluated LLMs.</p><p><strong>Conclusions: </strong>While newer iterations of LLMs, such as GPT-4o and Gemini Advanced, outperformed their less advanced counterparts (GPT-4o mini and Gemini), this study emphasizes the need for caution in clinical applications of these models. The choice of prompting techniques significantly influences performance, highlighting the necessity for further research to refine LLMs capabilities, particularly in visual data interpretation, to ensure their safe integration into medical practice.</p>","PeriodicalId":9606,"journal":{"name":"Canadian journal of ophthalmology. Journal canadien d'ophtalmologie","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143037245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-03DOI: 10.1016/j.jcjo.2024.12.001
Lee-Wing Nathan, Stockl Frank, Wiens James
Objective: To assess the cataract surgery (CS) complication rates before and during the COVID-19 pandemic.
Design: Retrospective, cross sectional study.
Methods: Medical records of patients undergoing CS at two surgery centers in Winnipeg from January 2018 to April 2022 were reviewed. The study periods were pre-COVID-19 (January 1, 2018 to March 31, 2020) and COVID-19 (April 1, 2020 to April 30, 2022). Complication rates for endophthalmitis, sulcus-positioned intraocular lens (IOL) implant, anterior chamber IOL (ACIOL) implant, capsular tension ring (CTR) insertion, cumulative dissipated phacoemulsification energy (CDE), posterior capsular rupture (PCR), unplanned anterior vitrectomy, and return to the OR within one month of surgery were compared.
Results: 45,559 patients were included. Pre-COVID-19 rates were compared to COVID-19 rates and stated as per 100 cataract surgeries. Endophthalmitis rates increased from 0.008 to 0.028 (RR 0.27, p=0.09). Sulcus IOL implant rates increased from 0.61 to 1.00 (RR 0.61, p<0.0001). ACIOL implant rates increased from 0.53 to 0.68 (RR 0.78, p=0.05). CTR usage rates increased from 0.48 to 0.75 (RR 0.63, p=0.001). Average CDE increased from 4.6 to 6.7 (p=0.0030). PCR rates decreased from 0.49 to 0.38 (RR 1.3, p=0.14). Unplanned anterior vitrectomy rates decreased from 0.43 to 0.36 (RR 1.18, p=0.35). Return to OR rates remained unchanged at 0.59 and 0.58 (RR 1.01, p=0.93).
Conclusions: Our study found significant increases in some CS complication rates when comparing the defined pre-COVID interval to the COVID interval.
{"title":"Complication rates in cataract surgery before and during the COVID-19 pandemic.","authors":"Lee-Wing Nathan, Stockl Frank, Wiens James","doi":"10.1016/j.jcjo.2024.12.001","DOIUrl":"https://doi.org/10.1016/j.jcjo.2024.12.001","url":null,"abstract":"<p><strong>Objective: </strong>To assess the cataract surgery (CS) complication rates before and during the COVID-19 pandemic.</p><p><strong>Design: </strong>Retrospective, cross sectional study.</p><p><strong>Methods: </strong>Medical records of patients undergoing CS at two surgery centers in Winnipeg from January 2018 to April 2022 were reviewed. The study periods were pre-COVID-19 (January 1, 2018 to March 31, 2020) and COVID-19 (April 1, 2020 to April 30, 2022). Complication rates for endophthalmitis, sulcus-positioned intraocular lens (IOL) implant, anterior chamber IOL (ACIOL) implant, capsular tension ring (CTR) insertion, cumulative dissipated phacoemulsification energy (CDE), posterior capsular rupture (PCR), unplanned anterior vitrectomy, and return to the OR within one month of surgery were compared.</p><p><strong>Results: </strong>45,559 patients were included. Pre-COVID-19 rates were compared to COVID-19 rates and stated as per 100 cataract surgeries. Endophthalmitis rates increased from 0.008 to 0.028 (RR 0.27, p=0.09). Sulcus IOL implant rates increased from 0.61 to 1.00 (RR 0.61, p<0.0001). ACIOL implant rates increased from 0.53 to 0.68 (RR 0.78, p=0.05). CTR usage rates increased from 0.48 to 0.75 (RR 0.63, p=0.001). Average CDE increased from 4.6 to 6.7 (p=0.0030). PCR rates decreased from 0.49 to 0.38 (RR 1.3, p=0.14). Unplanned anterior vitrectomy rates decreased from 0.43 to 0.36 (RR 1.18, p=0.35). Return to OR rates remained unchanged at 0.59 and 0.58 (RR 1.01, p=0.93).</p><p><strong>Conclusions: </strong>Our study found significant increases in some CS complication rates when comparing the defined pre-COVID interval to the COVID interval.</p>","PeriodicalId":9606,"journal":{"name":"Canadian journal of ophthalmology. Journal canadien d'ophtalmologie","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.jcjo.2024.05.019
Abdullah Al-Ani , Mohamed S Bondok , Kian Madjedi , Shellina Kherani , Amin Kherani
Objectif
Analyser l’évaluation et la prise en charge des corps étrangers intraoculaires (CEIO) en milieu urbain au Canada pour générer une perspective clinique précieuse afin de replacer la prise en charge dans son contexte.
Méthodes
Examen rétrospectif des dossiers médicaux des patients d'un seul chirurgien datés de janvier 2002 à janvier 2023 comprenant les caractéristiques démographiques de patients qui ont eu un CEIO, leurs examens, leurs traitements, leurs complications et leur meilleure acuité visuelle corrigée (MAVC).
Résultats
Notre étude a porté sur la présence d'un CEIO dans 32 yeux de 31 patients (96,8 % des patients étaient de sexe masculin). La taille du CEIO variait de 1 à 12 mm; 28 corps étrangers (87,5 %) étaient en métal; il s'agissait d'un accident de travail dans 15 cas (46,9 %). Dans le cadre de l'examen diagnostique, 19 patients (61,3 %) ont fait l'objet d'une tomographie assistée par ordinateur (TAO), et 8 patients (25,8 %), d'une échographie oculaire (B-scan). La TAO a permis de déceler les CEIO dans 100 % des cas, et les B-scans, dans 87,5 % des cas. Lors du suivi final, la MAVC de 17 yeux (53,1 %) était ≥ 20/40, comparativement à 7 yeux (23,3 %) au départ. Une MAVC ≥ 20/200 lors de l'examen initial était associée à une MAVC finale ≥ 20/40 (p = 0,027). Le CEIO a pu être retiré dans 27 yeux (84,4 %), est demeuré en place dans 4 yeux (12,5 %) et a entraîné l’énucléation de 1 œil (3,1 %). Des antibiotiques ont été administrés par voie intravitréenne dans 19 yeux (59,4 %), ce qui a donné lieu à 1 cas présumé de toxicité médicamenteuse. Des complications sont survenues dans 30 yeux (93,8 %) pour un total de 119, dont 72 (60,5 %) sont apparues pendant les 24 premières heures. La cataracte post-traumatique était la complication la plus fréquente (27 yeux; 84,4 %). Les complications moins fréquentes comprenaient la sidérose oculaire et le décollement de la rétine s'accompagnant d'une vitréorétinopathie proliférante (chacune ayant touché 1 œil; 3,1 %). Enfin, un glaucome secondaire est apparu dans 4 yeux (12,5 %), dont 3 où le CEIO était demeuré en place ou avait été extrait tardivement.
Conclusions
Les caractéristiques des CEIO et les paramètres démographiques des patients cadrent avec ceux d'autres régions. La TAO était l'outil d'examen le plus efficace. On recommande un suivi prolongé pour surveiller les complications, surtout en cas de rétention du CEIO ou lorsque son extraction est significativement retardée.
{"title":"Clinical outcomes and characterization of intraocular foreign body injuries from a Canadian centre: a 20-year retrospective study and literature review","authors":"Abdullah Al-Ani , Mohamed S Bondok , Kian Madjedi , Shellina Kherani , Amin Kherani","doi":"10.1016/j.jcjo.2024.05.019","DOIUrl":"10.1016/j.jcjo.2024.05.019","url":null,"abstract":"<div><h3>Objectif</h3><div>Analyser l’évaluation et la prise en charge des corps étrangers intraoculaires (CEIO) en milieu urbain au Canada pour générer une perspective clinique précieuse afin de replacer la prise en charge dans son contexte.</div></div><div><h3>Méthodes</h3><div>Examen rétrospectif des dossiers médicaux des patients d'un seul chirurgien datés de janvier 2002 à janvier 2023 comprenant les caractéristiques démographiques de patients qui ont eu un CEIO, leurs examens, leurs traitements, leurs complications et leur meilleure acuité visuelle corrigée (MAVC).</div></div><div><h3>Résultats</h3><div>Notre étude a porté sur la présence d'un CEIO dans 32 yeux de 31 patients (96,8 % des patients étaient de sexe masculin). La taille du CEIO variait de 1 à 12 mm; 28 corps étrangers (87,5 %) étaient en métal; il s'agissait d'un accident de travail dans 15 cas (46,9 %). Dans le cadre de l'examen diagnostique, 19 patients (61,3 %) ont fait l'objet d'une tomographie assistée par ordinateur (TAO), et 8 patients (25,8 %), d'une échographie oculaire (B-scan). La TAO a permis de déceler les CEIO dans 100 % des cas, et les B-scans, dans 87,5 % des cas. Lors du suivi final, la MAVC de 17 yeux (53,1 %) était ≥ 20/40, comparativement à 7 yeux (23,3 %) au départ. Une MAVC ≥ 20/200 lors de l'examen initial était associée à une MAVC finale ≥ 20/40 (<em>p</em> = 0,027). Le CEIO a pu être retiré dans 27 yeux (84,4 %), est demeuré en place dans 4 yeux (12,5 %) et a entraîné l’énucléation de 1 œil (3,1 %). Des antibiotiques ont été administrés par voie intravitréenne dans 19 yeux (59,4 %), ce qui a donné lieu à 1 cas présumé de toxicité médicamenteuse. Des complications sont survenues dans 30 yeux (93,8 %) pour un total de 119, dont 72 (60,5 %) sont apparues pendant les 24 premières heures. La cataracte post-traumatique était la complication la plus fréquente (27 yeux; 84,4 %). Les complications moins fréquentes comprenaient la sidérose oculaire et le décollement de la rétine s'accompagnant d'une vitréorétinopathie proliférante (chacune ayant touché 1 œil; 3,1 %). Enfin, un glaucome secondaire est apparu dans 4 yeux (12,5 %), dont 3 où le CEIO était demeuré en place ou avait été extrait tardivement.</div></div><div><h3>Conclusions</h3><div>Les caractéristiques des CEIO et les paramètres démographiques des patients cadrent avec ceux d'autres régions. La TAO était l'outil d'examen le plus efficace. On recommande un suivi prolongé pour surveiller les complications, surtout en cas de rétention du CEIO ou lorsque son extraction est significativement retardée.</div></div>","PeriodicalId":9606,"journal":{"name":"Canadian journal of ophthalmology. Journal canadien d'ophtalmologie","volume":"60 1","pages":"Pages e83-e91"},"PeriodicalIF":3.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141878441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.jcjo.2023.10.024
Sarah B. Pike , Mark W Reid , Chen-Ching Peng , Christina Chang , Benjamin Y. Xu , Dan S. Gombos , Sapna Patel , Liya Xu , Jesse L. Berry
<div><h3>Objective</h3><div><span>Uveal melanoma<span><span> (UM) tumour biopsy is limited by size and intratumour heterogeneity. We explored the potential of </span>aqueous humour (AH) </span></span>liquid biopsy<span> for UM by quantifying analytes in samples collected at diagnosis and after brachytherapy to look for clinical correlations with tumour features.</span></div></div><div><h3>Design</h3><div>Case-series study.</div></div><div><h3>Participants</h3><div>Sixty-six UM patients and 16 control subjects from a tertiary care hospital.</div></div><div><h3>Methods</h3><div><span><span>The study included 119 UM AH samples and 16 control samples analyzed for unprocessed analytes (i.e., dsDNA, </span>miRNA, and protein) using </span>Qubit<span> fluorescence assays.</span></div></div><div><h3>Results</h3><div><span>Analytes were widely quantifiable among available UM AH samples (dsDNA: 94.1%; miRNA: 88.0%; protein: 95.2%) at significantly higher concentrations than among control samples (dsDNA, </span><em>p</em><span> = 0.008; miRNA, </span><em>p</em> < 0.0001; protein, <em>p</em><span><span> = 0.007). In samples taken at diagnosis, concentrations were higher at more advanced American Joint Cancer Commission stages; when comparing most advanced stage III with least advanced stage I, median </span>dsDNA was 4 times greater (</span><em>p</em> < 0.0001), miRNA was 2 times greater (<em>p</em> = 0.001), and protein was 3 times greater (<em>p</em> < 0.0001). Analytes were quantifiable in >70% of diagnostic samples from eyes with tumours <2 mm tall. Height had a positive association with diagnostic analyte concentrations (dsDNA: <em>R</em> = 0.43, <em>p</em> = 0.0007; miRNA: <em>R</em> = 0.35, <em>p</em> = 0.01; protein: <em>R</em> = 0.39, <em>p</em> = 0.005). Samples taken after brachytherapy showed significantly higher concentrations than diagnostic samples (<em>p</em> < 0.01 for all).</div></div><div><h3>Conclusions</h3><div>UM AH is a rich repository of analytes. Samples from eyes with more advanced stage and larger tumours had higher concentrations, though analytes also were quantifiable in eyes with smaller, less advanced tumours. Future analysis of AH analytes may be informative in the pursuit of personalized UM treatments.</div></div><div><h3>Objectif</h3><div>En présence d'un mélanome de l'uvée (MU), la biopsie de la tumeur est limitée par sa taille et par l'hétérogénéité intratumorale. Nous avons examiné l'intérêt de la biopsie liquide de l'humeur aqueuse (HA) dans le MU en quantifiant les analytes dans les échantillons recueillis au moment du diagnostic et après la curiethérapie afin de faire ressortir des corrélations cliniques en lien avec les caractéristiques de la tumeur.</div></div><div><h3>Nature</h3><div>Série de cas.</div></div><div><h3>Participants</h3><div>L’étude portait sur 66 patients présentant un MU et 16 sujets témoins provenant d'un hôpital de soins tertiaires.</div></div><div><h3>Méthodes</h3><div>L’étude co
目的:葡萄膜黑色素瘤(UM)肿瘤活检受大小和肿瘤内异质性的限制。我们通过对诊断时和近距离放疗后收集的样本中的分析物进行定量分析,以寻找与肿瘤特征的临床相关性,探索了房水(AH)液体活检对UM的潜力。设计:案例系列研究。参与者:66名UM患者和16名来自三级护理医院的对照受试者。方法:本研究包括119份UM AH样品和16份对照样品,使用量子比特荧光法分析未处理的分析物(即dsDNA、miRNA和蛋白质)。结果:在现有UM AH样品中,分析物可广泛定量(dsDNA: 94.1%;microrna: 88.0%;蛋白:95.2%),显著高于对照样品(dsDNA, p = 0.008;miRNA, p < 0.0001;蛋白质、p = 0.007)。在诊断时采集的样本中,在美国联合癌症委员会的晚期,浓度更高;当比较最晚期III期和最晚期I期时,dsDNA的中位数是4倍(p < 0.0001), miRNA的中位数是2倍(p = 0.001),蛋白的中位数是3倍(p < 0.0001)。结论:umah是一个丰富的分析物库。晚期肿瘤和较大肿瘤的眼睛样本的浓度更高,尽管在较小的、不太严重的肿瘤的眼睛中也可以定量分析。未来对AH分析物的分析可能为追求个性化UM治疗提供信息。
{"title":"Multicentre analysis of nucleic acid quantification using aqueous humour liquid biopsy in uveal melanoma: implications for clinical testing","authors":"Sarah B. Pike , Mark W Reid , Chen-Ching Peng , Christina Chang , Benjamin Y. Xu , Dan S. Gombos , Sapna Patel , Liya Xu , Jesse L. Berry","doi":"10.1016/j.jcjo.2023.10.024","DOIUrl":"10.1016/j.jcjo.2023.10.024","url":null,"abstract":"<div><h3>Objective</h3><div><span>Uveal melanoma<span><span> (UM) tumour biopsy is limited by size and intratumour heterogeneity. We explored the potential of </span>aqueous humour (AH) </span></span>liquid biopsy<span> for UM by quantifying analytes in samples collected at diagnosis and after brachytherapy to look for clinical correlations with tumour features.</span></div></div><div><h3>Design</h3><div>Case-series study.</div></div><div><h3>Participants</h3><div>Sixty-six UM patients and 16 control subjects from a tertiary care hospital.</div></div><div><h3>Methods</h3><div><span><span>The study included 119 UM AH samples and 16 control samples analyzed for unprocessed analytes (i.e., dsDNA, </span>miRNA, and protein) using </span>Qubit<span> fluorescence assays.</span></div></div><div><h3>Results</h3><div><span>Analytes were widely quantifiable among available UM AH samples (dsDNA: 94.1%; miRNA: 88.0%; protein: 95.2%) at significantly higher concentrations than among control samples (dsDNA, </span><em>p</em><span> = 0.008; miRNA, </span><em>p</em> < 0.0001; protein, <em>p</em><span><span> = 0.007). In samples taken at diagnosis, concentrations were higher at more advanced American Joint Cancer Commission stages; when comparing most advanced stage III with least advanced stage I, median </span>dsDNA was 4 times greater (</span><em>p</em> < 0.0001), miRNA was 2 times greater (<em>p</em> = 0.001), and protein was 3 times greater (<em>p</em> < 0.0001). Analytes were quantifiable in >70% of diagnostic samples from eyes with tumours <2 mm tall. Height had a positive association with diagnostic analyte concentrations (dsDNA: <em>R</em> = 0.43, <em>p</em> = 0.0007; miRNA: <em>R</em> = 0.35, <em>p</em> = 0.01; protein: <em>R</em> = 0.39, <em>p</em> = 0.005). Samples taken after brachytherapy showed significantly higher concentrations than diagnostic samples (<em>p</em> < 0.01 for all).</div></div><div><h3>Conclusions</h3><div>UM AH is a rich repository of analytes. Samples from eyes with more advanced stage and larger tumours had higher concentrations, though analytes also were quantifiable in eyes with smaller, less advanced tumours. Future analysis of AH analytes may be informative in the pursuit of personalized UM treatments.</div></div><div><h3>Objectif</h3><div>En présence d'un mélanome de l'uvée (MU), la biopsie de la tumeur est limitée par sa taille et par l'hétérogénéité intratumorale. Nous avons examiné l'intérêt de la biopsie liquide de l'humeur aqueuse (HA) dans le MU en quantifiant les analytes dans les échantillons recueillis au moment du diagnostic et après la curiethérapie afin de faire ressortir des corrélations cliniques en lien avec les caractéristiques de la tumeur.</div></div><div><h3>Nature</h3><div>Série de cas.</div></div><div><h3>Participants</h3><div>L’étude portait sur 66 patients présentant un MU et 16 sujets témoins provenant d'un hôpital de soins tertiaires.</div></div><div><h3>Méthodes</h3><div>L’étude co","PeriodicalId":9606,"journal":{"name":"Canadian journal of ophthalmology. Journal canadien d'ophtalmologie","volume":"60 1","pages":"Pages e23-e31"},"PeriodicalIF":3.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138458103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}