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The impact of COVID-19 infection on thyroid function. COVID-19感染对甲状腺功能的影响。
IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-12 DOI: 10.14715/cmb/2024.70.12.19
Esraah Alharris, Dina Saleh, Thair Wali Ali

Extensive research on COVID-19 has revealed a notable link between the disease and thyroid disorders, highlighting complex interactions between thyroid hormones, immunomodulatory signaling molecules within the thyroid gland, and viral infections. This study evaluated the relationship between thyroid function and COVID-19 in Iraqi patients at Adiwaniyah Teaching Hospital. The cohort for this investigation comprised all patients who were admitted to the isolation center at the Teaching Hospital during the timeframe extending from January 2024 to June 2024. Each participant included in this research underwent comprehensive evaluations of their thyroid function, which is composed of the measurement of thyroid-stimulating hormone (TSH), total triiodothyronine (T3), and serum total thyroxine (T4) levels. Results showed that the serum T4 levels in all participants included in the study were observed to range from 20 to 182 (ng/dl), with the average concentration recorded at 87.26 ± 38.29 (ng/dl); no statistically significant disparity was noted in the mean serum T4 levels relative to the severity of the disease (p = 0.291). The serum TSH levels across all enrolled individuals spanned from 0.03 to 82 (mU/L), with a mean concentration of 5.55 ± 12.36 (mU/L); similarly, there was no statistically significant difference in the mean serum TSH levels when assessed against the disease severity (p = 0.926). According to the serum thyroid hormone concentrations, the cohort was stratified into 17 (24.6%) individuals classified as hypothyroid, 34 (49.3%) categorized as euthyroid, and 18 (26.1%) identified as hyperthyroid. Furthermore, no significant correlation was identified between the disease's severity and the participants' thyroid status (p = 0.556). In conclusion, patients with COVID-19 are liable to develop thyroid function abnormalities that may explain several of the long-term symptoms associated with the disease.

对COVID-19的广泛研究揭示了该疾病与甲状腺疾病之间的显著联系,强调了甲状腺激素、甲状腺内免疫调节信号分子和病毒感染之间的复杂相互作用。本研究评估了Adiwaniyah教学医院伊拉克患者甲状腺功能与COVID-19的关系。本调查的队列包括2024年1月至2024年6月期间在教学医院隔离中心住院的所有患者。本研究对每位参与者进行了甲状腺功能的综合评估,包括促甲状腺激素(TSH)、总三碘甲状腺原氨酸(T3)和血清总甲状腺素(T4)水平的测量。结果:所有研究对象血清T4水平在20 ~ 182 (ng/dl)之间,平均为87.26±38.29 (ng/dl);血清T4水平与疾病严重程度的差异无统计学意义(p = 0.291)。所有受试者的血清TSH水平范围为0.03 ~ 82 (mU/L),平均浓度为5.55±12.36 (mU/L);同样,当评估疾病严重程度时,平均血清TSH水平无统计学差异(p = 0.926)。根据血清甲状腺激素浓度,该队列分为17例(24.6%)甲状腺功能低下,34例(49.3%)甲状腺功能正常,18例(26.1%)甲状腺功能亢进。此外,疾病的严重程度与参与者的甲状腺状态之间没有明显的相关性(p = 0.556)。总之,COVID-19患者容易出现甲状腺功能异常,这可以解释与该疾病相关的一些长期症状。
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引用次数: 0
Assessment of bacteriological and immunological markers in urinary tract infection and the effect of antibiotics on the isolated bacteria. 尿路感染细菌学和免疫学指标的评估及抗生素对分离细菌的影响。
IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-12 DOI: 10.14715/cmb/2024.70.12.17
Wisam F Hameed, Bashar S Noomi, Alaa A Khaleel, Dhouha Ghribi

Urinary tract infections (UTIs) are recognized as the second most common medical condition, following respiratory infections. Despite the availability of numerous efficacious antibiotics for the management of UTIs, the rising incidence of bacterial resistance presents significant challenges in the treatment of these infections. Bacteria are endowed with the ability to reproduce and develop resistance mechanisms against antibiotics. The current investigation aimed to evaluate the susceptibility of bacterial isolates from urinary tract infections (UTIs) to a variety of antibiotics, including ciprofloxacin, trimethoprim, amikacin, gentamicin, tetracycline, chloramphenicol, nalidixic acid, nitrofurantoin, meropenem, and novobiocin. Additionally, the study sought to quantify the levels of the inflammatory immune marker interleukin-6 (IL-6) in UTI patients. It also explored the correlation between IL-6 levels in UTI patients and healthy controls, as well as the relationship between IL-6 levels and blood parameters in both infected and healthy individuals. The present study involved the collection of 155 samples from patients diagnosed with urinary tract infections of both genders and varying age groups, ranging from 15 to 75 years, at Salah al-Din General Hospital. The findings revealed that 102 urine samples tested positive for bacterial growth, resulting in a prevalence rate of 68%. In contrast, 53 urine samples were negative for bacterial growth, reflecting a prevalence rate of 32%. The diagnostic outcomes for all isolates, following the application of laboratory diagnostic methodologies, revealed a diverse array of bacterial species, including Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, Staphylococcus saprophyticus, Staphylococcus aureus, and Staphylococcus epidermidis. The immunological analysis revealed a statistically significant increase (p < 0.05) in IL-6 concentrations in the positive control group compared to the levels observed in the infected cohort. Our study concluded that significant antibiotic resistance in UTI pathogens, emphasizing the need for tailored treatments.

尿路感染(UTI)被认为是继呼吸道感染之后的第二大常见疾病。尽管目前有多种有效的抗生素可用于治疗尿路感染,但细菌耐药性的不断增加给这些感染的治疗带来了巨大挑战。细菌具有繁殖和发展抗生素耐药性机制的能力。本次调查旨在评估尿路感染(UTI)细菌分离物对多种抗生素的敏感性,包括环丙沙星、三甲氧苄啶、阿米卡星、庆大霉素、四环素、氯霉素、萘啶酸、硝基呋喃妥因、美罗培南和新生物素。此外,该研究还试图量化尿毒症患者的炎症免疫标志物白细胞介素-6(IL-6)的水平。研究还探讨了UTI 患者和健康对照组 IL-6 水平之间的相关性,以及感染者和健康人 IL-6 水平与血液参数之间的关系。本研究在萨拉赫丁总医院收集了 155 份尿液样本,这些样本来自 15 至 75 岁不同年龄段的尿路感染患者,其中包括男性和女性。研究结果显示,102 份尿液样本的细菌生长呈阳性,患病率为 68%。相比之下,53 份尿样的细菌生长呈阴性,患病率为 32%。应用实验室诊断方法对所有分离物进行诊断的结果显示,细菌种类繁多,包括大肠埃希菌、肺炎克雷伯菌、变形杆菌、铜绿假单胞菌、溶血性葡萄球菌、金黄色葡萄球菌和表皮葡萄球菌。免疫学分析表明,阳性对照组的 IL-6 浓度与感染组相比有显著的统计学增长(p < 0.05)。我们的研究得出结论,UTI 病原体对抗生素具有明显的耐药性,这强调了采取针对性治疗的必要性。
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引用次数: 0
Pharmacognistic, proximate and phytochemical analysis of stem of Cistanche tubulosa (Schenck) Hook. F. 肉苁蓉茎的生药学、近似值及植物化学分析。F。
IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-12 DOI: 10.14715/cmb/2024.70.12.4
Maryum Nousheen, Abdul Basit, Muhammad Kamran Khan, Rasha M Alzayed, Sondos A Alhajouj, Meaad F Alaida, Awatif M E Omran, Abdulrahman Alasmari, Ahmad El Askary, Riyadh S Almalki, Mohamed M Zayed

A medicinal plant is any plant that in one or more of its organs contains substances that can be used by it or their constituent for therapeutic purposes. The present work was done to evaluate pharmacognostic, fluorescence, proximate and phytochemical analysis of ethanolic extracts of Cistanche tubulosa (Orobanchaceae) along with antimicrobial activity. Antimicrobial activity against four bacterial strains S. aureus, E. coli, P. aeruginosa, S. typhi along with five fungal strains such as F. oxyfurum, P. notatum, Candida albicans, A. fumigatus, and A. niger evaluate using agar well diffusion method. The powder drug study of various tissues of plants revealed higher concentrations (20.29 mg/l) of essential macro and micronutrients. Fluorescence analysis of the stem powdered with various chemical reagents showed different colors. Proximate analysis showed the presence of crude substances such as proteins (8.5 %), fat (1.5 %), fibres (6.6 %), carbohydrates (73.87 %), moisture contents (3.23 %) and ash contents (6.3 %) respectively. Phytochemical screening revealed the presence of carbohydrates, proteins and dozen other important secondary metabolites. The presence of these bioactive constituents associated with the antimicrobial activity of S. aureus showed the maximum zone of inhibition (15.1 ± 3.7 mm), while in antifungal activity C. albicans showed the highest zone of inhibition (11.0 ± 3.15 mm). The pharmacognostic study, fluorescence analysis and antimicrobial activity are helpful in the standardization of the drug establishing a good support for the use of C. tubulosa in traditional medicine.

药用植物是指在其一个或多个器官中含有可用于治疗目的的物质或其成分的任何植物。本研究对肉苁蓉(大戟科)乙醇提取物的药理、荧光、近物和植物化学分析以及抗菌活性进行了评估。使用琼脂井扩散法评估了对四种细菌菌株金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌、伤寒杆菌以及五种真菌菌株的抗菌活性,如F. oxyfurum、P. notatum、Candida albicans、A. fumigatus 和 A. niger。对植物各种组织进行的粉末药物研究显示,必需的宏量和微量营养素的浓度较高(20.29 毫克/升)。用各种化学试剂对茎粉进行的荧光分析显示出不同的颜色。近似分析表明存在粗物质,如蛋白质(8.5 %)、脂肪(1.5 %)、纤维(6.6 %)、碳水化合物(73.87 %)、水分含量(3.23 %)和灰分含量(6.3 %)。植物化学筛选显示出了碳水化合物、蛋白质和其他十几种重要的次生代谢物。这些生物活性成分的存在与金黄色葡萄球菌的抗菌活性有关,其抑制面积最大(15.1 ± 3.7 毫米),而在抗真菌活性方面,白僵菌的抑制面积最大(11.0 ± 3.15 毫米)。药理研究、荧光分析和抗菌活性有助于药物的标准化,为在传统医学中使用 C. tubulosa 提供了良好的支持。
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引用次数: 0
Relationships between genetic polymorphisms of IL-1β and rheumatoid arthritis. IL-1β基因多态性与类风湿关节炎的关系
IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-12 DOI: 10.14715/cmb/2024.70.12.13
Nawres Adnan Abdulameer

This study aimed to investigate the association between the interleukin-1 beta (IL-1β) gene polymorphism (rs2853550) and the risk of rheumatoid arthritis (RA) in a sample of the Iraqi population. The study included 100 RA patients and 100 healthy controls. Demographic characteristics, including age and gender, were collected and compared between the two groups. The IL-1β (rs2853550) polymorphism was genotyped using the ARMS-PCR method. The distribution of genotypes and alleles of the IL-1β (rs2853550) polymorphism was significantly different between RA patients and healthy controls. The frequency of the heterozygous AG genotype was significantly higher in the patient group compared to the control group (33% vs. 25%, p=0.001). The odds ratio for RA in individuals with the AG genotype was 1.5038 (95% CI: 0.7274-3.1086), indicating that it may be a potential risk factor. Additionally, the frequency of the G allele was significantly higher in RA patients compared to controls (129 vs. 109, p=0.0021), with an odds ratio of 1.5169 (95% CI: 1.0151-2.2667). The present study demonstrates that the IL-1β (rs2853550) polymorphism is associated with an increased risk of rheumatoid arthritis in the Iraqi population. The AG genotype and the G allele of this polymorphism may serve as genetic markers for susceptibility to RA.

本研究旨在调查伊拉克人群样本中白细胞介素-1β (IL-1β)基因多态性(rs2853550)与类风湿关节炎(RA)风险之间的关系。该研究包括100名RA患者和100名健康对照者。收集人口统计学特征,包括年龄和性别,并在两组之间进行比较。采用ARMS-PCR方法对IL-1β (rs2853550)多态性进行基因分型。IL-1β (rs2853550)多态性的基因型和等位基因分布在RA患者和健康对照组之间存在显著差异。与对照组相比,患者组的AG基因型杂合子频率显著高于对照组(33% vs. 25%, p=0.001)。AG基因型患者RA的优势比为1.5038 (95% CI: 0.7274-3.1086),提示其可能是一个潜在的危险因素。此外,RA患者中G等位基因的频率显著高于对照组(129比109,p=0.0021),优势比为1.5169 (95% CI: 1.0151-2.2667)。目前的研究表明,IL-1β (rs2853550)多态性与伊拉克人群类风湿关节炎风险增加有关。AG基因型和该多态性的G等位基因可作为类风湿关节炎易感性的遗传标记。
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引用次数: 0
Variants of the ABCG2 gene in Mexican mestizo patients with prostate cancer. 墨西哥混血前列腺癌患者的ABCG2基因变异
IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-12 DOI: 10.14715/cmb/2024.70.12.25
Gabriela Monserrat Mimendi-Aguilar, Michael Dean, Silvia Esperanza Flore-Martínez, Alejandra Guadalupe García-Zapien, José Sánchez-Corona, María Fernanda Romero-Morán, Martha Arisbeth Villanueva-Pérez, Erick Sierra-Díaz, Salvador Sánchez-Benavides, Ingrid Patricia Dávalos-Rodríguez, Jesús Alejandro Juárez-Ozuna, Mónica Alejandra Rosales-Reynoso, Maria Cristina Morán Moguel

ABCG2 transporter protein is one of several markers of prostate cancer stem cells (PCSCs). Gene variants of ABCG2 could affect protein expression, function, or both. The aim of this study was to identify the genetic variability of the ABCG2 gene in Mexican patients with prostate cancer. Genomic DNA (gDNA) was obtained from peripheral blood samples of 32 Mexican patients with prostate cancer. ABCG2 gene was sequenced. The electropherograms were analyzed using mutation surveyor DNA mutation analysis software (Softgenetics). The ABCG2 gene sequence revealed the presence of 22 variants: 19 previously described and three previously undescribed gene variants as part of the ABCG2 gene variability in the Mexican mestizo population (R263K G>A, R378K G>A, and Q531Q G>A). No ABCG2 variant was identified in one patient, but 1 to 12 variants were identified in the remaining 31 patients. The transition G>A was the most frequently found substitution. The largest number of ABCG2 variants was located in exon 9, and at least one of them was present in 28 of the 31 subjects in the Mexican population. The individual genetic variability of ABCG2 should be analyzed, considering its possible usefulness in personalized medicine in patients with prostate cancer.

ABCG2转运蛋白是前列腺癌干细胞(PCSCs)的标志物之一。ABCG2基因变异可能影响蛋白表达、功能或两者兼而有之。本研究的目的是确定墨西哥前列腺癌患者ABCG2基因的遗传变异性。从32例墨西哥前列腺癌患者的外周血样本中获得基因组DNA (gDNA)。测定ABCG2基因序列。用突变测量员DNA突变分析软件(Softgenetics)对电泳图进行分析。ABCG2基因序列揭示了22个变体的存在:19个先前描述的和3个先前未描述的基因变体作为墨西哥混血儿群体ABCG2基因变异性的一部分(R263K G>A, R378K G>A和Q531Q G>A)。1例患者未发现ABCG2变异,但在其余31例患者中发现了1至12种变异。跃迁g>a是最常发现的取代。ABCG2变异数量最多的是位于第9外显子,在墨西哥人群的31名受试者中有28人存在至少一种变异。考虑到ABCG2在前列腺癌患者个体化治疗中的可能作用,应分析其个体遗传变异性。
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引用次数: 0
Vitamin E improves the reproductive system of male rats exposed to busulfan chemotherapy. 维生素E可以改善接受布硫凡化疗的雄性大鼠的生殖系统。
IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-12 DOI: 10.14715/cmb/2024.70.12.24
Ameer Hameed Kadhim, Mehdi El Arbi, Hayder Ali Muhammed

Vitamin E is a well-known antioxidant and is frequently used as an adjunct treatment in cancer therapy. Busulfan is a commonly used drug for cancer treatment. In this study, twenty-eight male rats, ten weeks old and weighing between 250 and 300 grams, were divided into four groups. The first group served as the control and received daily intraperitoneal injections of Dimethyl sulfoxide (DMSO) for four weeks. The second group received a single dose of Busulfan at 40 mg/kg body weight via intraperitoneal injection. The third group received the same single dose of Busulfan along with daily intraperitoneal injections of Vitamin E at 100 mg/kg body weight for four weeks. The fourth group was given only Vitamin E at the same dosage for four weeks. At the end of the experiment, the animals were sacrificed, and blood samples were collected to test antioxidant enzyme levels (GSH, SOD, CAT) and analyze serum concentrations of reproductive hormones (FSH, LH, and testosterone). Additionally, sperm motility and viability were assessed after collecting epididymal spermatozoa. The findings revealed that Busulfan significantly increased serum levels of both FSH and LH while causing a notable decrease in testosterone levels. Furthermore, Busulfan treatment resulted in a significant reduction in sperm count, motility, and viability, along with a marked increase in sperm morphological abnormalities. In contrast, supplementation with Vitamin E alongside Busulfan improved hormone levels and enhanced sperm function. In conclusion, Busulfan has a toxic effect on sperm and directly impacts body weight and testicular weight. However, Vitamin E demonstrates beneficial therapeutic effects on testicular tissue and enhances sperm production in rats treated with Busulfan.

维生素E是一种众所周知的抗氧化剂,经常被用作癌症治疗的辅助治疗。布磺胺是一种常用的癌症治疗药物。在这项研究中,28只10周龄、体重在250到300克之间的雄性大鼠被分成四组。第一组为对照组,连续4周每天腹腔注射二甲亚砜(DMSO)。第二组腹腔注射单剂量40 mg/kg体重的布硫凡。第三组给予相同单剂量的布苏凡,同时每天腹腔注射维生素E,剂量为100 mg/kg体重,连续四周。第四组连续四周只服用相同剂量的维生素E。实验结束后,处死动物,采集血液,检测抗氧化酶(GSH、SOD、CAT)水平,分析血清生殖激素(FSH、LH、睾酮)浓度。此外,收集附睾精子后评估精子活力和活力。结果显示,Busulfan显著提高血清FSH和LH水平,同时显著降低睾酮水平。此外,Busulfan治疗导致精子数量、活力和活力显著减少,同时精子形态异常显著增加。相比之下,补充维生素E和Busulfan可以提高激素水平,增强精子功能。综上所述,Busulfan对精子有毒性作用,并直接影响体重和睾丸重量。然而,维生素E显示有益的治疗作用,对睾丸组织和提高精子生产的大鼠治疗布苏凡。
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引用次数: 0
Association between extracellular matrix protein 1 (ECM1) gene polymorphisms (rs3834087 and rs3754217) and Hepatitis B Virus evolution in an African cohort. 非洲人群中细胞外基质蛋白1 (ECM1)基因多态性(rs3834087和rs3754217)与乙型肝炎病毒进化的关系
IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-12 DOI: 10.14715/cmb/2024.70.12.5
Lassina Traore, Minane Nafissa Triande, Sidnooma Véronique Zongo, Abdoul Karim Ouattara, Mousso Savadogo, Nouhoun Nignan, Marie Simone Traore, Tegwindé Rébéca Compaore, Denise P Ilboudo, Tani Sagna, Ina Marie Traore, Birama Diarra, Albert Théophane Yonli, Bolni Marius Nagalo, Augustin Tozoula Bambara, Roger Arsène Sombie, Florencia Wendkuuni Djigma, Jacques Simpore

Hepatitis B virus (HBV) is a significant cause of liver disease and cancer worldwide. Understanding the genetic factors influencing HBV evolution is crucial for developing effective prevention and treatment strategies. Host genetic and environmental factors particularly influence the evolution of this infection. Recent studies have implicated the ECM1 gene in HBV pathogenesis, mainly two specific polymorphisms (rs3834087 and rs3754217). In an African cohort, we comprehensively analyzed these ECM1 gene polymorphisms and their association with HBV evolution.In this case-control analysis, 167 samples, consisting of 59 controls and 108 cases, were examined. The cases included 50 patients with Chronic Hepatitis B(CHB), 16 with cirrhosis, and 42 with hepatocellular carcinoma (HCC). Genomic DNA extraction was executed using INVITROGEN and FAVORGEN kits. Genotyping of rs3834087 and rs3754217 polymorphisms in the ECM1 gene was accomplished via real-time PCR on the QuantStudioTM 5 Real-Time instrument, followed by allelic discrimination using TaqMan Genotyper Software. Data was interpreted using SPSS version 20 and Epi info version 7.5.2.0. Odds ratios (OR), confidence intervals (CI), and p-values were derived for risk and significance evaluation.In our study, the heterozygous genotype (GT) of rs3754217 could confer protection to controls against the onset of chronic hepatitis in the event of infection (OR=0.05; CI=0.006-0.46; p=0.002). In addition, carriage of mutated alleles of the two (2) polymorphisms was associated with the course of infection and may influence the appearance of severe forms at certain stages of the disease.Our study is the first to assess the association between polymorphisms (rs3834087 and rs3754217) in the ECM1 gene and the course of HBV infection in Burkina Faso. It showed that combining specific genotypes of the two (2) polymorphisms would be associated with protection against chronic hepatitis.

乙型肝炎病毒(HBV)是世界范围内肝脏疾病和癌症的重要原因。了解影响HBV进化的遗传因素对于制定有效的预防和治疗策略至关重要。宿主遗传和环境因素特别影响这种感染的演变。最近的研究表明ECM1基因与HBV的发病机制有关,主要是两种特异性多态性(rs3834087和rs3754217)。在一个非洲队列中,我们全面分析了这些ECM1基因多态性及其与HBV进化的关系。在本病例-对照分析中,共检查了167份样本,包括59份对照和108例病例。这些病例包括50例慢性乙型肝炎(CHB), 16例肝硬化,42例肝细胞癌(HCC)。使用INVITROGEN和FAVORGEN试剂盒进行基因组DNA提取。在QuantStudioTM 5实时荧光定量PCR仪上对ECM1基因rs3834087和rs3754217多态性进行基因分型,然后使用TaqMan基因分型软件进行等位基因鉴定。数据分析采用SPSS version 20和Epi info version 7.5.2.0。比值比(OR)、置信区间(CI)和p值用于风险和显著性评估。在我们的研究中,rs3754217的杂合基因型(GT)可以在感染的情况下保护对照者免受慢性肝炎的发作(OR=0.05;CI = 0.006 - -0.46;p = 0.002)。此外,携带两(2)个多态性的突变等位基因与感染过程有关,并可能影响疾病某些阶段严重形式的出现。我们的研究首次评估了布基纳法索ECM1基因多态性(rs3834087和rs3754217)与HBV感染过程之间的关系。结果表明,结合两(2)个多态性的特定基因型可能与慢性肝炎的保护有关。
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引用次数: 0
Clinical and molecular analysis of ESBL, carbapenemase, and colistin-resistant bacteria in UTI patients. 尿路感染患者ESBL、碳青霉烯酶和粘菌素耐药菌的临床和分子分析。
IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-12 DOI: 10.14715/cmb/2024.70.12.23
Farooq Ali, Qismat Shakeela, Shehzad Ahmed, Rahat Ullah Khan, Johar Jamil, Pir Tariq Shah, Tayyaba Afsar, Ali Almajwal, Suhail Razak, Hazrat Bilal

Uropathogens, particularly bacteria, can infect any part of the urinary tract and cause bacteriuria. Our study aimed to examine the antibiotic-resistant profile, associated risk factors, and phenotypic and genotypic features of ESBL, carbapenemase, and mcr resistance genes in multidrug-resistant bacteria. Samples were inoculated on culture media, identified using standard biochemical tests, and species confirmation was performed via 16S rRNA gene amplification. Furthermore, antibiotic susceptibilities were evaluated using the Kirby-Bauer disc diffusion method. The phenotypically confirmed resistant strains were further inspected for ESBL, carbapenemases, and mcr variants using PCR. Merely 57.24% (83/145) of the samples exhibited growth. Of these, 39.70% (33/83) were identified as Klebsiella pneumoniae, 27.70% (23/83) as Escherichia coli, 10.80% (9/83) as Pseudomonas aeruginosa, 9.60% (8/83) as Staphylococcus aureus, 7.20% (6/83) as Proteus mirabilis, and 4.80% (4/83) as Staphylococcus saprophyticus. Overall, 22.54% (16/71) of the gram-negative strains were confirmed molecularly to have resistant genes. The ESBL - producers accounted for 21.74% (5/23) of E. coli, 21.21% (7/33) of K. pneumoniae, and 22.22% (2/9) of P. aeruginosa. Likewise, carbapenemase-harboring strains included 6.06% (2/33) of K. pneumoniae, 4.35% (1/23) of E. coli, and 11.11% (1/9) of P. aeruginosa. Notably, 3.03% (1/33) of K. pneumoniae, 8.70% (2/23) of E. coli, and 11.11% (1/9) of P. aeruginosa strains tested positive for the mcr-1 gene. None of the Proteus strains showed any resistant genes. The most common variants were blaSHV-11 (non-ESBL) and blaCTX-M-15 (ESBL) accounted for 28.57% (4/14) each, blaTEM-116 accounted for 14.29% (2/14), blaSHV-1, blaSHV-75, blaTEM-1 and blaOXA-1 accounted for 7.14% (1/14) each of the ESBL. Similarly, the carbapenemase variants included blaOXA-48, blaNDM-1, blaVIM-1, and blaKPC-2, each accounting for 25.0% (1/4), while 37.50% (6/16) of the strains exhibited co-existence of different gene variants. Based on our findings, it can be concluded that females, especially those in middle age, were more infected. These pathogens exhibited a wide range of ESBL, carbapenemase, and mcr-1 variants. Imipenem was suggested as the preferred medication.

尿路病原体,特别是细菌,可以感染尿路的任何部位并引起细菌性尿症。本研究旨在研究多重耐药细菌中ESBL、碳青霉烯酶和mcr耐药基因的耐药特征、相关危险因素以及表型和基因型特征。将样品接种于培养基上,采用标准生化试验鉴定,并通过16S rRNA基因扩增进行物种确认。此外,采用Kirby-Bauer圆盘扩散法评估抗生素敏感性。表型证实的耐药菌株使用PCR进一步检测ESBL、碳青霉烯酶和mcr变异。只有57.24%(83/145)的样品生长。其中肺炎克雷伯菌占39.70%(33/83),大肠杆菌占27.70%(23/83),铜绿假单胞菌占10.80%(9/83),金黄色葡萄球菌占9.60%(8/83),神奇变形杆菌占7.20%(6/83),腐生葡萄球菌占4.80%(4/83)。总体而言,22.54%(16/71)的革兰氏阴性菌株经分子鉴定具有耐药基因。大肠杆菌产生ESBL的占21.74%(5/23),肺炎克雷伯菌占21.21%(7/33),铜绿假单胞菌占22.22%(2/9)。含碳青霉烯酶菌株中肺炎克雷伯菌占6.06%(2/33),大肠杆菌占4.35%(1/23),铜绿假单胞菌占11.11%(1/9)。其中,3.03%(1/33)的肺炎克雷伯菌、8.70%(2/23)的大肠杆菌和11.11%(1/9)的铜绿假单胞菌的mcr-1基因检测呈阳性。没有一个变形杆菌菌株显示出任何抗性基因。最常见的变异是blaSHV-11(非ESBL)和blaCTX-M-15 (ESBL)各占28.57% (4/14),blaTEM-116占14.29% (2/14),blaSHV-1、blaSHV-75、blaTEM-1和blaOXA-1各占7.14%(1/14)。同样,碳青霉烯酶变异包括blaOXA-48、blaNDM-1、blaVIM-1和blaKPC-2,各占25.0%(1/4),37.50%(6/16)的菌株存在不同基因变异。根据我们的研究结果,可以得出结论,女性,特别是中年女性,更容易感染。这些病原体表现出广泛的ESBL、碳青霉烯酶和mcr-1变体。建议将亚胺培南作为首选药物。
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引用次数: 0
The relationship of serum vaspin level with clinical parameters in patients with fibromyalgia syndrome. 纤维肌痛综合征患者血清vaspin水平与临床参数的关系。
IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-12-20 DOI: 10.14715/cmb/2024.70.11.6
Muhammet Şahin Elbastı, Emine Kaçar

Vaspin plays a regulatory role in lipid and glucose metabolism and is a therapeutic adipokine against impaired glucose intolerance in obese individuals. We aimed to investigate serum vaspin levels in patients with FMS and whether there was any relationship between vaspin levels and metabolic and clinical parameters in fibromyalgia. A total of 64 female patients who applied to an outpatient clinic due to widespread pain lasting more than three months were included in the study. The patients were divided into two groups: 32 in the fibromyalgia group and 32 in the healthy controls. The socio-demographic characteristics of the patients were evaluated with the standard evaluation form. Age, weight, height, blood pressure, body mass index (BMI), waist circumference, presence of menopause were recorded. Pain intensity was evaluated with visual analogue scale (VAS). The Fibromyalgia Impact Scale (FIS) was utilized to measure quality of life and functional status. Metabolic syndrome components were significantly different in the fibromyalgia group compared to the control group (p <0.05). While 22 patients (68.8%) in the fibromyalgia group met the diagnostic criteria for metabolic syndrome, three patients (9.4%) in the control group met these criteria. In the fibromyalgia intra-group correlation, vaspin was significantly positively correlated with BMI and waist circumference (p<0.05). In the control group, vaspin indicated a statistically significant positive correlation with BMI. This study elaborated that waist circumference, insulin, and insulin resistance were significantly higher in the fibromyalgia patients compared to the healthy control group. This was confirmed by the finding that significantly more patients met the diagnostic criteria for metabolic syndrome. Additionally, vaspin was considerably higher in fibromyalgia patients and thus it was positively correlated with BMI and waist circumference.

Vaspin在脂质和葡萄糖代谢中起调节作用,是一种治疗性脂肪因子,可对抗肥胖个体的糖耐受性受损。我们的目的是研究FMS患者的血清vaspin水平,以及vaspin水平与纤维肌痛患者的代谢和临床参数之间是否存在关系。共有64名因持续3个月以上的广泛疼痛而申请门诊的女性患者被纳入研究。患者被分为两组:32名纤维肌痛组和32名健康对照组。采用标准量表对患者的社会人口学特征进行评估。记录年龄、体重、身高、血压、身体质量指数(BMI)、腰围、是否绝经。采用视觉模拟评分法(VAS)评价疼痛强度。采用纤维肌痛影响量表(FIS)测量患者的生活质量和功能状态。与对照组相比,纤维肌痛组代谢综合征成分有显著差异(p
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引用次数: 0
Impact of COVID-19 infection on cyclooxygenase-2 gene expression and mast cell count in testicular tissue of azoospermic men. COVID-19感染对无精子男性睾丸组织环氧化酶-2基因表达和肥大细胞计数的影响
IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-12-04 DOI: 10.14715/cmb/2024.70.11.11
Zahra Kalhor, Azra Allahvaisi, Mohammad Jafar Rezaie, Rezgar Daneshdust, Bahram Nikkhoo, Khaled Rahmani

The COVID-19 pandemic has posed significant threats to human life and health. Numerous studies have shown that men are more vulnerable to this infection, and recent evidence suggests that the presence of angiotensin-converting enzyme 2 (ACE2) receptors in male reproductive tissues may particularly predispose them to viral infection. Therefore, it is crucial to assess the potential impact of COVID-19 infection on male fertility. This study investigates the relationship between COVID-19 and the expression of inflammatory proteins, particularly mast cells and cyclooxygenase-2 (COX-2), in the testicular tissue of azoospermic men undergoing testicular sperm extraction (TESE). The study included 41 TESE candidates who were referred to the Besat Infertility Treatment Center in Kurdistan, Iran. Demographic information, such as age, was recorded for each participant. The subjects were divided into two groups: 20 non-infected and 21 infected with COVID-19. Testicular tissue samples were fixed in formalin and prepared for microscopic examination using toluidine blue staining and immunohistochemistry to assess the distribution and number of mast cells and COX-2 positive cells. Data analysis was performed using SPSS software version 27. The results showed that COX-2 gene expression and the number of mast cells were significantly higher in individuals infected with COVID-19 compared to the non-infected group. This increase in gene expression and mast cell count indicates elevated inflammation in the testicular tissue of COVID-19-infected individuals, which could lead to reduced fertility. This study aligns with previous research highlighting the role of inflammation in testicular tissue damage and decreased fertility.

新冠肺炎疫情给人类生命健康带来重大威胁。大量研究表明,男性更容易受到这种感染,最近的证据表明,男性生殖组织中血管紧张素转换酶2 (ACE2)受体的存在可能使他们特别容易受到病毒感染。因此,评估COVID-19感染对男性生育能力的潜在影响至关重要。本研究探讨了COVID-19与接受睾丸精子提取(TESE)的无精子男性睾丸组织中炎症蛋白,特别是肥大细胞和环氧化酶-2 (COX-2)表达的关系。该研究包括41名TESE候选人,他们被转介到伊朗库尔德斯坦的Besat不孕症治疗中心。每个参与者的年龄等人口统计信息都被记录下来。受试者分为两组:20名未感染和21名感染。将睾丸组织样本固定在福尔马林中,用甲苯胺蓝染色和免疫组织化学进行显微镜检查,以评估肥大细胞和COX-2阳性细胞的分布和数量。数据分析采用SPSS软件27版。结果显示,与未感染组相比,感染COVID-19的个体的COX-2基因表达和肥大细胞数量显著增加。基因表达和肥大细胞计数的增加表明,covid -19感染者睾丸组织炎症升高,这可能导致生育能力下降。这项研究与先前的研究一致,强调炎症在睾丸组织损伤和生育能力下降中的作用。
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引用次数: 0
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