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Antiproliferative effects mechanism of β-sitosterul in hepatoma HepG2 cells β-谷甾醇对肝癌HepG2细胞的抗增殖作用机制
Pub Date : 2011-08-01 DOI: 10.4268/CJCMM20111529
Zhong-quan Zhang, Yujun Xing, Guo-qiang Hu, Songqiang Xie
OBJECTIVE To study the antiproliferative effects of beta-sitosterul and its mechanism in hepatoma HepG2 cells. METHOD Cell proliferation was assessed by MTT assay. Cell cycle distribution, apoptosis and mitochondrial membrane potential were measured by high content screening (HCS). The protein expression of caspase-3, caspase-8, caspase-9, Bcl-2, Bax, tBid and cytochrome c in the HepG2 cells were evaluated by Western Blots. RESULT beta-Sitosterul exerted significant antiproliferative effects in HepG2 cells. Furthermore, beta-sitosterul also induced HepG2 cells apoptosis, lost mitochondrial membrane potential, activated caspase-3, caspase-8 and caspase-9, up-regulate Bax, tBid protein, down-regulation Bcl-2 protein. However, beta-sitosterul had hardly any effects on QSG7701 cells. CONCLUSION beta-Sitosterul exerted antiproliferative effects and induced HepG2 cells apoptosis via mitochondrial pathway and membrane death receptor pathway.
目的研究β -谷甾醇对肝癌HepG2细胞的抗增殖作用及其机制。方法采用MTT法检测细胞增殖情况。采用高含量筛选法(HCS)检测细胞周期分布、凋亡及线粒体膜电位。Western Blots检测HepG2细胞中caspase-3、caspase-8、caspase-9、Bcl-2、Bax、tBid和细胞色素c的表达。结果β -西妥妥对HepG2细胞有明显的抗增殖作用。此外,β -谷甾醇还能诱导HepG2细胞凋亡,线粒体膜电位丧失,激活caspase-3、caspase-8和caspase-9,上调Bax、tBid蛋白,下调Bcl-2蛋白。然而,β -sitosterul对QSG7701细胞几乎没有任何影响。结论β -西妥妥可通过线粒体途径和膜死亡受体途径诱导HepG2细胞凋亡。
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引用次数: 2
Summary of revised contents of medical standards of preparation recorded in Chinese Pharmacopeia 中国药典制剂医学标准修订内容摘要
Pub Date : 2011-07-15 DOI: 10.4268/CJCMM20111434
Bai Xiaoju
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引用次数: 2
Advance of polysorbate 80 for injection accessories 注射配件用聚山梨酯80的研制进展
Pub Date : 2011-07-01 DOI: 10.4268/CJCMM20111415
Zhang Meiyu
: Polysorbate 80 for injection accessories is one of the main reactions of clinical adverse to which may be leaded by a variety of traditional Chinese medicine injection. In this review, on the basis of literature research and experimental studies, polysorbate 80 are summarized in areas which conclude the chemical structure, basic nature,quality standards, pharmacological activity, safety evaluation and toxicity studies. The review aimed to provide theoretical basis for the rational selections of Chinese medicine, safe and efficient usages of injection accessories, and to promote healthy development of traditional Chinese medicine injections.
聚山梨酯80是各种中药注射剂引起的临床主要不良反应之一。本文在文献研究和实验研究的基础上,对聚山梨酯80的化学结构、基本性质、质量标准、药理活性、安全性评价和毒性研究等方面进行了综述。旨在为合理选用中药、安全高效使用注射辅料提供理论依据,促进中药注射剂的健康发展。
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引用次数: 2
Reseach on changes of varieties of ancient and modern Chinese herbs 古今中草药品种变化研究
Pub Date : 2011-07-01 DOI: 10.4268/CJCMM20111328
Tang Decai
Because of many reasons, big changes occur in the varieties of ancient and modern Chinese herbs. Besides a significant increase in the number, there are many other features, such as significant differences among mainstream varieties, partly resolving the chaotic situation of uncoincidence of different herb names, increasing proportion of plant herbs, and herbal multi-polarization, etc.
由于种种原因,古今中草药的品种发生了很大的变化。除数量显著增加外,还有主流品种间差异显著,部分解决了不同药名不重合的混乱局面,植物类药材比例增加,药材多极化等特点。
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引用次数: 0
Analysis of content changes of volatile oil and β-elemene in oil in Sarcandrae 菝葜属精油挥发油及β-榄香烯含量变化分析
Pub Date : 2011-06-01 DOI: 10.4268/CJCMM20111221
Suxiang Wu, Shengna Zhang, Suhong Chen, Zhaohuan Lou, Guiyuan Lv
OBJECTIVE To reveal the factors that affect the contents of volatile oil and beta-elemene in oil in Sarcandrae and to provide scientific basis for Sarcandrae's reasonable exploitation and quality assessment. METHOD A GC method was established and the contents of volatile oil and beta-elemene were determined in Sarcandrae from different length of time to grow, different medicament portions, different collection periods and different length of time to dry. RESULT The length of time to grow had no significant effect and the collection periods had effect on the volatile oil yield and content of beta-elemene. The volatile oil yield and content of beta-elemene were the highest in Sarcandrae harvested in December. The yield of volatile oil in different medicament portions descended in an order of roots, leaves and stems. The content of beta-elemene was the highest in leaves and the lowest in stems. With the increasing of the length of time to dry, the volatile oil yield and content of beta-elemene decreased calculated on an anhydrous basis. CONCLUSION The established method is simple, accurate and repeatable. It can be used for the quality control of beta-elemene in Sarcandrae. The study provides a valuable basis for the quality assessment, the development and utilization of Sarcandrae.
目的揭示影响菝葜精油挥发油和β -榄香烯含量的因素,为菝葜药材的合理开发利用和质量评价提供科学依据。方法建立气相色谱法,测定不同生长时间、不同给药部位、不同采收期和不同干燥时间下菝葜挥发油和β -榄香烯的含量。结果生长时间对挥发油收率和β -榄香烯含量无显著影响,采收时间对挥发油收率和β -榄香烯含量有显著影响。12月采收的菝葜挥发油产量和β -榄香烯含量最高。不同药剂部位挥发油得率按根、叶、茎依次递减。β -榄香烯含量以叶片最高,茎部最低。在无水条件下,随着干燥时间的延长,挥发油得率和β -榄香烯含量降低。结论所建立的方法简便、准确、重复性好。该方法可用于菝葜中-榄香烯的质量控制。该研究为菝葜属植物的质量评价和开发利用提供了有价值的依据。
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引用次数: 1
[Treatment of premenstrual syndrome with Chinese medicine formula, baixiangdan capsule: implications for neuron viability and GABA(A) receptor modulation in rat cortex]. [中药方剂、白香丹胶囊治疗经前期综合征:对大鼠皮层神经元活力及GABA受体调节的影响]。
Pub Date : 2011-06-01 DOI: 10.4268/CJCMM20111122
Sun Shiguang
OBJECTIVE: To explore the effects of Baixiangdan capsule (BXD), a Chinese medicinal formula, on the premenstrual syndrome (PMS) rats with Liver-qi invasion and the possible underlying micro-mechanisms. METHOD: Wistar rats were randomly assigned to two groups, nomal group and BXD group After evaluated by macro-behavior observation and open-field test, MTT assay and the whole-cell patch clamp recording were performed respectively to evaluate the effects of serum from BXD capsule-treated rats on the viability and GABA(A) R-induced currents of cortical neurons in vitro. RESULT: In the open-field test, the crossing score, rearing score and total score of BXD rats decreased significantly (P < 0.05), compared by the normal rats. Compared with cells exposed to serum of normal rats, the viability values of those incubated with serum of BXD group for 24 h and 48 h significantly increased (P < 0.05), measured by MTT assay. The results of whole-cell patch clamp recording showed that concentration-response relationship curves revealed an EC50 value of (29.0 +/- 4.4) micromol x L(-1) and a Hill coefficient of 1.07 for normal-exposed cultures, (63.5 +/- 8.2) micromol x L(-1), 1.04 for BXD-exposed cultures after incubation for 24 h. Furthermore, the difference in EC50 values was statistically significant (P < 0.01), that in the Hill coefficient was not obvious. CONCLUSION: BXD capsule could significantly decrease crossing score and total score of open-field test and effectively enhance the neuron viability and GABA(A) receptor activity in rat cortex. Paeonimetabolins I and paeonol may play a significant role in treating PMS model rats with Liver-qi invasion by BXD capsule, and paeonol may target at GABA(A) receptor, especially.
目的:探讨中药方剂白香丹胶囊(BXD)对经前症候群(PMS)肝气侵大鼠的影响及其可能的微观机制。方法:将Wistar大鼠随机分为正常组和BXD组,分别采用宏观行为学观察、开场实验、MTT法和全细胞膜片钳法观察BXD胶囊处理大鼠血清对体外皮质神经元活力和GABA(A) r诱导电流的影响。结果:与正常大鼠相比,BXD大鼠的杂交评分、饲养评分和总分均显著降低(P < 0.05)。MTT法测定,与正常大鼠血清相比,BXD组细胞孵育24 h和48 h的细胞活力值显著升高(P < 0.05)。全细胞膜片钳记录结果显示,培养24 h后,正常暴露培养的EC50值为(29.0 +/- 4.4)micromol x L(-1), Hill系数为1.07,bxd暴露培养的EC50值为(63.5 +/- 8.2)micromol x L(-1), Hill系数为1.04,差异有统计学意义(P < 0.01), Hill系数差异不明显。结论:BXD胶囊能显著降低大鼠开场试验交叉评分和总分,有效提高大鼠皮层神经元活力和GABA(A)受体活性。芍药代谢素I和丹皮酚可能在BXD胶囊治疗PMS模型大鼠肝气侵害中发挥重要作用,且丹皮酚可能以GABA(a)受体为靶点。
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引用次数: 2
Study on chemical constituents from branches and leaves of Polyalthia nemoralis 毛茛枝叶化学成分的研究
Pub Date : 2011-04-01 DOI: 10.4268/CJCMM20110817
Lu Ziming
OBJECTIVE To investigate the chemical constituents of the branches and leaves of Polyalthia nemoralis. METHOD The compounds were isolated and purified by silica gel, macroporous adsorption resin and Sephadex LH-20 column chromatographic methods. Their chemical structures were elucidated on the basis of physicochemical properties and spectral data. RESULT Fourteen compounds were isolated and identified as syringic acid (1), 3-methoxy-4-hydroxycinnamic acid (2), vanillic acid (3), 4-hydroxybenzoic acid (4), mauritianin (5), (+)-xylopinidine (6), (+)-oblongine(7), (+)-tembetarine (8), eythritol (9), D-mannitol (10), ethyl-beta-D-glucopyranoside (11), (+)-magnoflorine (12), stepharanine (13), (2S, 4R)-4-hydroxy-2-piperidine-carboxylic acid (14), respectively. CONCLUSION All the compounds were isolated from the genus Polyalthia for the first time; compounds 6 and 13 showed inhibitation activities against multi tumor cell lines.
目的研究毛茛枝叶的化学成分。方法采用硅胶、大孔吸附树脂和Sephadex LH-20柱层析分离纯化化合物。根据理化性质和光谱数据对其化学结构进行了分析。结果共分离得到14个化合物,分别鉴定为丁香酸(1)、3-甲氧基-4-羟基肉桂酸(2)、香草酸(3)、4-羟基苯甲酸(4)、毛柳香苷(5)、(+)-木氯吡啶(6)、(+)-长叶碱(7)、(+)-丹betarine(8)、乙四醇(9)、d -甘露醇(10)、乙基- β - d -葡萄糖吡喃苷(11)、(+)-木兰花碱(12)、stephananine(13)、(2S, 4R)-4-羟基-2-哌酸(14)。结论所有化合物均为首次从该属植物中分离得到;化合物6和13对多种肿瘤细胞系均有抑制作用。
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引用次数: 1
Composition and thermal stability of traditional Tibetan mineral medicine Nanhanshuishi 藏药南寒水石的组成及热稳定性研究
Pub Date : 2011-03-01 DOI: 10.4268/CJCMM20110607
Quan Zhengxiang
OBJECTIVE: To determine the composition, structure, trace elements and thermal stability of Tibetan medicine Nanhanshuishi. METHOD: The trace elements, the structure, and the thermal stability of Nanhanshuishi were assayed and calculated by X-ray fluorescence spectrometry (XRF), inductively coupled plasma optical emission spectrometer (ICP-OES), atomic fluorescence spectrometry (AFS), inductively coupled plasma mass spectrometry (ICP-MS), X-ray power diffraction (XRD), TG-DTA. RESULT: The results indicated that the phase is mainly made up of CaCO3 (Rhombohedral, R-3c) in Nanhanshuishi. The analysis of elements show that Nanhanshuishi is rich in Ca and O, and contains other more than 20 minor elements, such as Si, Mg, Fe, Al, Na, K, Zn, Mn, Pb, As, Hg etc. The result of TG-DTA show that the weight of Nanhanshuishi starts to decline from near 700 degrees C and get steady above 850 degrees C. CONCLUSION: The study provided scientific data for the establishment of quality standards of Tibetan medicine Nanhanshuishi.
目的:测定藏药南寒水石的组成、结构、微量元素及热稳定性。方法:采用x射线荧光光谱法(XRF)、电感耦合等离子体光学发射光谱仪(ICP-OES)、原子荧光光谱法(AFS)、电感耦合等离子体质谱法(ICP-MS)、x射线功率衍射仪(XRD)、TG-DTA对南寒水石的微量元素、结构和热稳定性进行测定和计算。结果:南汉水石的相主要由CaCO3 (Rhombohedral, R-3c)组成。元素分析表明,南汉水石富含Ca和O,并含有Si、Mg、Fe、Al、Na、K、Zn、Mn、Pb、as、Hg等20多种微量元素。TG-DTA结果表明,在700℃附近,南寒水石的质量开始下降,在850℃以上趋于稳定。结论:本研究为藏药南寒水石质量标准的制定提供了科学依据。
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引用次数: 1
Effects of soil water content on seedlings growth and active ingredients of Salvia miltiorrhiza 土壤含水量对丹参幼苗生长及有效成分的影响
Pub Date : 2011-02-01 DOI: 10.4268/CJCMM20110320
Liu Dahui
OBJECTIVE: In order to provide a scientific water management for the standardized cultivation, the effects of soil water content on the seedlings growth and active ingredients of S. miltiorrhiza were studied. METHOD: A water stress experiment with pot culture was applied to study the effects of different soil water content on the seedlings growth, biomass and the accumulation of tanshinone, salvianolic acid B and mineral nutrition of S. miltiorrhiza. RESULT: Soil water content had serious influence on the growth, yield, outer appearance and inner quality of S. miltiorrhiza when the soil was under severe drought or with too much water. But the shoot and root biomass of S. miltiorrhiza was increased significantly under mild drought. As well as the content and cumulant of dihydrotanshinone I , cryptotanshinone, tanshinone I , tanshinone IIA and salvianolic acid B in root of mild drought were increased. It also enhanced the P, K, Ca, Mg, Mn, Zn, Cu and Fe contents of S. miltiorrhiza under mild drought. CONCLUSION: The overall results from the experiment suggest that the appropriate soil water content is 55% to 60% in seedling stage of S. miltiorrhiza. And it will be strongly recommended that the ridge culture and suitable soil moisture management must be carry out in production of S. miltiorrhiza in order to improve the yield and quality of medicinal materials.
目的:研究土壤含水量对丹参幼苗生长及有效成分的影响,为丹参标准化栽培提供科学的水分管理方法。方法:采用盆栽水分胁迫试验,研究不同土壤含水量对丹参幼苗生长、生物量以及丹参酮、丹酚酸B和矿质营养积累的影响。结果:在严重干旱或水分过多的土壤条件下,土壤含水量对丹参的生长、产量、外观和内在品质均有严重影响。但在轻度干旱条件下,丹参的茎、根生物量显著增加。轻度干旱根中二氢丹参酮I、隐丹参酮、丹参酮I、丹参酮IIA和丹酚酸B的含量和累积量均增加。在轻度干旱条件下,对丹参的P、K、Ca、Mg、Mn、Zn、Cu和Fe含量也有提高作用。结论:综合试验结果,丹参苗期土壤含水量以55% ~ 60%为宜。建议在丹参生产中实行垄作栽培和适宜的土壤水分管理,以提高丹参药材的产量和质量。
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引用次数: 6
Study on 11 mental element contents in Dendrobium officinale 铁皮石斛中11种金属元素含量的研究
Pub Date : 2011-02-01 DOI: 10.4268/CJCMM20110128
Zhu Yan
{"title":"Study on 11 mental element contents in Dendrobium officinale","authors":"Zhu Yan","doi":"10.4268/CJCMM20110128","DOIUrl":"https://doi.org/10.4268/CJCMM20110128","url":null,"abstract":"","PeriodicalId":9835,"journal":{"name":"China Journal of Chinese Matera Medica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84285070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
期刊
China Journal of Chinese Matera Medica
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