Parkinson′s disease(PD) is a neurodegenerative disorder with a complex,multifactorial aetiology.The brains of patients affected with PD are characterized by a loss of neurons in dopamine neurons in the substantia nigra,decreasing of dopamine secretion,and the deposition of Lewy bodies(LBs) in the cytoplasm of remaining neurons.In China the data show that the incidence of Parkinson′s disease increases at least 20 times in recent 20 years,and it makes things worse for the aging society.Developing good anti-PD drugs to improve the patient′s quality of life is particularly important.The treatment of PD using traditional Chinese medicine(TCM) has made remarkable effect,while the the molecular mechanisms of it is still not known,while elucidating the molecular mechanismof TCM is the base of better understanding its function.Using genetically modified PD model of Caenorhabditis elegans,which is suitable for molecular mechanism study,to explore the interference mechanism of TCM to PD might be an effective way.This review briefly introduces the research progress on molecular mechanism of PD,and then discusses the idea of using C.elegans to study molecular mechanism of TCM intervention to PD.
{"title":"[Study of molecular mechanism of anti-Parkinson's disease traditional Chinese medicine using model of Caenorhabditis elegans].","authors":"Wang Xiangming, W. Xiaoyan, Gao Huili, W. Danqiao","doi":"10.4268/cjcmm20100527","DOIUrl":"https://doi.org/10.4268/cjcmm20100527","url":null,"abstract":"Parkinson′s disease(PD) is a neurodegenerative disorder with a complex,multifactorial aetiology.The brains of patients affected with PD are characterized by a loss of neurons in dopamine neurons in the substantia nigra,decreasing of dopamine secretion,and the deposition of Lewy bodies(LBs) in the cytoplasm of remaining neurons.In China the data show that the incidence of Parkinson′s disease increases at least 20 times in recent 20 years,and it makes things worse for the aging society.Developing good anti-PD drugs to improve the patient′s quality of life is particularly important.The treatment of PD using traditional Chinese medicine(TCM) has made remarkable effect,while the the molecular mechanisms of it is still not known,while elucidating the molecular mechanismof TCM is the base of better understanding its function.Using genetically modified PD model of Caenorhabditis elegans,which is suitable for molecular mechanism study,to explore the interference mechanism of TCM to PD might be an effective way.This review briefly introduces the research progress on molecular mechanism of PD,and then discusses the idea of using C.elegans to study molecular mechanism of TCM intervention to PD.","PeriodicalId":9835,"journal":{"name":"China Journal of Chinese Matera Medica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79900678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
OBJECTIVE To investigate the effects of Ginkgo biloba extract (GbE) on the glucose uptake rate and gene expression of glucose transporter 4 (GLUT4) in diaphragm of diabetic rats. METHOD Forty SD male rats were randomly divided into normal control group (n=10) and model group (n=30). Diabetic models were induced by feeding with high-sucrose-high-fat diet and intraperitoneal injecting 25 mg X kg(-1) streptozotocin. 20 successful models were rearranged to two groups: diabetic group and GbE treatment group, 10 rats in each. Then the saline and 8 mg X kg(-1) x d(-1) of GbE were respectively intraperitoneal injected, once a day continuously for 8 weeks. The contents of fasting blood glucose (FBG), fasting insulin (FINS) were detected, respectively. The glucose uptake rate and gene expression of GLUT4 in diaphragm were determinated and the varieties of diaphragm ultrastructure were observed. RESULT Compared with control group, levels of FBG and FINS obviously increased in diabetic rats (P < 0.01), but the glucose uptake rate and expression of GLUT4 mRNA in diaphragm decreased significantly (P < 0.05, P < 0.01). The ultrastructure in diabetic group under electron microscope indicated that diaphragm mitochondrion swelled and degenerated. The above changes were inhibited by GbE. CONCLUSION GbE can improve the glucose metabolism in diabetic rats and reduce the diabetes-induced diaphragm damage. The action mechanism of the drug may be related to promote the mRNA expression of GLUT4 in diaphragm and improve the uptake and metabolism of blood glucose.
目的探讨银杏叶提取物(GbE)对糖尿病大鼠膈肌葡萄糖摄取率及葡萄糖转运蛋白4 (GLUT4)基因表达的影响。方法40只SD雄性大鼠随机分为正常对照组(n=10)和模型组(n=30)。采用高糖高脂饲料喂养，腹腔注射链脲佐菌素25 mg X kg(-1)，建立糖尿病模型。将20只成功模型重新分为糖尿病组和GbE治疗组，每组10只。然后分别腹腔注射GbE生理盐水8 mg X kg(-1) X d(-1)，每天1次，连续8周。分别测定各组空腹血糖(FBG)、空腹胰岛素(FINS)含量。测定大鼠横膈膜葡萄糖摄取率和GLUT4基因表达，观察横膈膜超微结构变化。结果与对照组相比，糖尿病大鼠FBG和FINS水平明显升高(P < 0.01)，膈肌葡萄糖摄取率和GLUT4 mRNA表达显著降低(P < 0.05, P < 0.01)。糖尿病组电镜下超微结构显示膈膜线粒体肿胀变性。GbE抑制了上述变化。结论GbE能改善糖尿病大鼠的糖代谢，减轻糖尿病引起的膈肌损伤。其作用机制可能与促进膈肌GLUT4 mRNA表达，改善血糖摄取和代谢有关。
{"title":"Effect of Ginkgo biloba extract on glucose uptake of diaphragm in diabetic rats","authors":"Li Xusheng","doi":"10.4268/CJCMM20100322","DOIUrl":"https://doi.org/10.4268/CJCMM20100322","url":null,"abstract":"OBJECTIVE To investigate the effects of Ginkgo biloba extract (GbE) on the glucose uptake rate and gene expression of glucose transporter 4 (GLUT4) in diaphragm of diabetic rats. METHOD Forty SD male rats were randomly divided into normal control group (n=10) and model group (n=30). Diabetic models were induced by feeding with high-sucrose-high-fat diet and intraperitoneal injecting 25 mg X kg(-1) streptozotocin. 20 successful models were rearranged to two groups: diabetic group and GbE treatment group, 10 rats in each. Then the saline and 8 mg X kg(-1) x d(-1) of GbE were respectively intraperitoneal injected, once a day continuously for 8 weeks. The contents of fasting blood glucose (FBG), fasting insulin (FINS) were detected, respectively. The glucose uptake rate and gene expression of GLUT4 in diaphragm were determinated and the varieties of diaphragm ultrastructure were observed. RESULT Compared with control group, levels of FBG and FINS obviously increased in diabetic rats (P < 0.01), but the glucose uptake rate and expression of GLUT4 mRNA in diaphragm decreased significantly (P < 0.05, P < 0.01). The ultrastructure in diabetic group under electron microscope indicated that diaphragm mitochondrion swelled and degenerated. The above changes were inhibited by GbE. CONCLUSION GbE can improve the glucose metabolism in diabetic rats and reduce the diabetes-induced diaphragm damage. The action mechanism of the drug may be related to promote the mRNA expression of GLUT4 in diaphragm and improve the uptake and metabolism of blood glucose.","PeriodicalId":9835,"journal":{"name":"China Journal of Chinese Matera Medica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91323069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
OBJECTIVE To investigate the space environment on the role of licorice mutagenesis analysis of proteins. METHOD Licorice (Glycyrrhiza uralensis) seeds were carried by a recoverable satellite for 18 days (the average radiation dose in the flight recovery module was 0.102 m x d(-1), the orbit semidiameter 350 km, gravity 10(-6)). After return, The satellite-flown seeds and the unflown seeds (ground control) were planted in the fields of experimental farm. The leaves of each group were used for studying the effects of space flight on CAT, SOD activity, the protein content and electrophoresis. RESULT After the space flight, CAT, SOD activity of licorice increased in varying degrees, the difference was significant (P<0.05), two types of enzyme activity of sample from Ordos were higher than that from Hangjinqi. The protein content of licorice increased in a certain extent, the difference was significant (P<0.05), while protein electrophoresis also showed differences, weak new bands appeared. CONCLUSION These results indicated that spaceflight has effect on protein of licorice, these changes may be used as a tool for accelerating the progress in G. uralensis breeding.
目的探讨空间环境对甘草蛋白诱变分析的作用。方法利用返回式卫星携带甘草种子18天(返回式卫星返回舱平均辐射剂量0.102 m x d(-1)，轨道半直径350 km，重力10(-6))。返回后，将卫星播撒的种子和未播撒的种子(地面对照)种在试验农场的田地里。以各组叶片为试验材料，研究了航天飞行对叶片CAT、SOD活性、蛋白质含量和电泳的影响。结果航天飞行后，甘草的CAT、SOD活性均有不同程度的升高，差异有统计学意义(P<0.05)，鄂尔多斯样品的两类酶活性均高于杭锦旗样品。甘草蛋白含量有一定程度的提高，差异显著(P<0.05)，蛋白电泳也有差异，出现了较弱的新条带。结论航天飞行对甘草蛋白有一定的影响，这些变化可作为加快乌拉尔氏甘草育种进程的工具。
{"title":"Effects of space flight on protein content and electrophoresis in Glycyrrhiza uralensis","authors":"Gao Wenyuan","doi":"10.4268/CJCMM20100201","DOIUrl":"https://doi.org/10.4268/CJCMM20100201","url":null,"abstract":"OBJECTIVE To investigate the space environment on the role of licorice mutagenesis analysis of proteins. METHOD Licorice (Glycyrrhiza uralensis) seeds were carried by a recoverable satellite for 18 days (the average radiation dose in the flight recovery module was 0.102 m x d(-1), the orbit semidiameter 350 km, gravity 10(-6)). After return, The satellite-flown seeds and the unflown seeds (ground control) were planted in the fields of experimental farm. The leaves of each group were used for studying the effects of space flight on CAT, SOD activity, the protein content and electrophoresis. RESULT After the space flight, CAT, SOD activity of licorice increased in varying degrees, the difference was significant (P<0.05), two types of enzyme activity of sample from Ordos were higher than that from Hangjinqi. The protein content of licorice increased in a certain extent, the difference was significant (P<0.05), while protein electrophoresis also showed differences, weak new bands appeared. CONCLUSION These results indicated that spaceflight has effect on protein of licorice, these changes may be used as a tool for accelerating the progress in G. uralensis breeding.","PeriodicalId":9835,"journal":{"name":"China Journal of Chinese Matera Medica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80699332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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