Biomedical Technology最新文献_第3页

Integrated bioinformatics analysis and machine learning approach for the identification of immune-related genes in the diagnosis of aortic valve calcification with periodontitis 综合生物信息学分析和机器学习方法在牙周炎主动脉瓣钙化诊断中的免疫相关基因鉴定

Biomedical Technology

Pub Date : 2025-06-01 DOI: 10.1016/j.bmt.2025.100087

Duolikun Mutailifu, Abudousaimi Aini, Abudunaibi Maimaitiaili

Background

Aortic valve calcification (AVC) represents a progressive, age-associated disorder characterized by substantial mortality, yet effective early diagnostic markers for AVC complicated by periodontitis, a common inflammatory condition linked to systemic inflammation, remain elusive. Our investigation sought to uncover immune-specific molecular indicators for AVC in patients with periodontitis using bioinformatics and machine learning.

Methods

Gene expression data for AVC (utilizing datasets GSE153555, GSE148219, GSE51472) and periodontitis (from dataset GSE16134) underwent analysis. We identified differentially expressed genes (DEGs) and determined the overlapped genes between AVC and periodontitis. The study included functional enrichment, protein-protein interaction (PPI) network construction, and immune infiltration analyses. To screen potential target genes, four machine learning models were developed (SVM, RF, XGB, GLM), with validation performed using an external dataset and clinical specimens via qRT-PCR.

Results

A total of 30 intersecting genes between AVC and periodontitis were identified. Four key genes—CXCL12, HCST, ITGA4, and GZMK—were selected through machine learning. The nomogram model combining these genes demonstrated high diagnostic accuracy, with an AUC of 0.985 in the training set and AUC values of 0.8, 0.72, 0.88, and 0.76 for HCST, ITGA4, CXCL12, and GZMK, respectively, in the external validation using the GSE51472 dataset. qRT-PCR validation in clinical samples confirmed that these genes were significantly upregulated in AVC patients with periodontitis. These genes were also correlated with immune cell infiltration, suggesting their potential role in AVC pathogenesis.

Conclusion

These findings provide new clinical molecular diagnostics, treatment related molecular markers for AVC in patients with periodontitis and may facilitate further basic research into biological functions.

主动脉瓣钙化（AVC）是一种进行性、与年龄相关的疾病，其特点是死亡率高，但AVC合并牙周炎（一种与全身性炎症相关的常见炎症）的有效早期诊断标志物仍然难以找到。我们的研究旨在利用生物信息学和机器学习揭示牙周炎患者AVC的免疫特异性分子指标。方法分析AVC（数据集GSE153555、GSE148219、GSE51472）和牙周炎（数据集GSE16134）的基因表达数据。我们鉴定了差异表达基因（DEGs），并确定了AVC和牙周炎之间的重叠基因。研究包括功能富集、蛋白相互作用（PPI）网络构建和免疫浸润分析。为了筛选潜在的靶基因，开发了四种机器学习模型（SVM， RF， XGB， GLM），并通过qRT-PCR使用外部数据集和临床标本进行验证。结果共鉴定出30个AVC与牙周炎的交叉基因。通过机器学习选择4个关键基因：cxcl12、HCST、ITGA4和gzmk。结合这些基因的nomogram model显示出较高的诊断准确率，在训练集中的AUC为0.985，在使用GSE51472数据集进行外部验证时，HCST、ITGA4、CXCL12和GZMK的AUC分别为0.8、0.72、0.88和0.76。临床样本的qRT-PCR验证证实，这些基因在伴有牙周炎的AVC患者中显著上调。这些基因也与免疫细胞浸润相关，提示它们在AVC发病机制中的潜在作用。结论本研究结果为牙周炎患者AVC提供了新的临床分子诊断和治疗相关分子标志物，并为进一步开展AVC生物学功能的基础研究奠定了基础。

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引用次数: 0

A comprehensive review of cancer drug nanoparticles synthesis, processing technology and its effect in drug delivery 综述了抗癌药物纳米颗粒的合成、加工技术及其在给药中的作用

Biomedical Technology

Pub Date : 2025-06-01 DOI: 10.1016/j.bmt.2025.100085

Mujibur Khan , Jannatul Ferdaus , Khaleda Akter , Hossain Ahmed , Mahrima Parvin , Sawaiz Kashif , Ali S. Arbab

The advent of nanotechnology has significantly advanced cancer treatment by introducing innovative approaches to targeted drug delivery and enhanced therapeutic efficiency. Nano drugs and nanocarriers, owing to their nanoscale dimensions, extensive surface area, and ability to modulate biodistribution, have proven effective in localizing tumor sites and enabling sustained drug release. These properties result in greater cytotoxicity and minimize systemic side effects compared to conventional therapies. Moreover, nanoparticles can be functionalized with molecular targeting agents, such as peptides, and combined with imaging dyes to improve the precision and monitoring of in-vivo treatments. A promising development in this domain is the utilization of biological carriers, particularly exosomes. These extracellular vesicles (30–150 nm in size) are secreted by various cells and possess a unique capacity to influence the tumor microenvironment through intercellular interactions and direct fusion with cell membranes. This review examines recent advancements in cancer drug delivery, with an emphasis on the design and processing of nano drugs and evaluates the potential of engineered exosomes as a transformative modality in cancer therapeutics.

纳米技术的出现通过引入创新的靶向给药方法和提高治疗效率，显著地推进了癌症治疗。纳米药物和纳米载体由于其纳米尺度的尺寸、广泛的表面积和调节生物分布的能力，已被证明在肿瘤部位定位和实现药物持续释放方面是有效的。与传统疗法相比，这些特性导致更大的细胞毒性和最大限度地减少全身副作用。此外，纳米颗粒可以与分子靶向剂（如肽）功能化，并与成像染料结合，以提高体内治疗的精度和监测。生物载体，特别是外泌体的利用是这一领域的一个有前景的发展。这些细胞外囊泡（30 - 150nm大小）由多种细胞分泌，具有通过细胞间相互作用和与细胞膜直接融合影响肿瘤微环境的独特能力。本文综述了癌症药物传递的最新进展，重点介绍了纳米药物的设计和加工，并评估了工程外泌体作为癌症治疗变革模式的潜力。

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引用次数: 0

Superparamagnetic hydrogels: Precision-driven platforms for biomedicine, robotics, and environmental remediation 超顺磁水凝胶：用于生物医学、机器人和环境修复的精密驱动平台

Biomedical Technology

Pub Date : 2025-06-01 DOI: 10.1016/j.bmt.2025.100084

Huaibin Wang , Yingying Hou , Long Chen , Weihong Mo , Leyan Xuan , Jialin Wu , Jie Wang , Maobin Xie , Shufang Wang , Guosheng Tang

Hydrogels are widely recognized for their biocompatibility and structural adaptability in regenerative medicine and three-dimensional (3D) bioprinting, yet their inherent static nature fundamentally limits applications demanding dynamic spatiotemporal control. The incorporation of superparamagnetic iron oxide nanoparticles (SPIONs) addresses this issue. The incorporation of SPIONs enables real-time programmable manipulation through magnetic field gradients. This amalgamation not only endows hydrogels with abilities such as magnetic propulsion, positioning, magnetoguidance, movement, and levitation, typical of magnetic materials, but also introduces novel functionalities like responsiveness to thermal effects and enhanced adsorption capabilities. This review delves into the transformative potential unlocked by the integration of SPIONs into hydrogels, showcasing their unique functional enhancements and targeted applications in robotics, precision medicine, and wastewater treatment.

水凝胶因其生物相容性和结构适应性在再生医学和三维生物打印中得到广泛认可，但其固有的静态特性从根本上限制了需要动态时空控制的应用。超顺磁性氧化铁纳米颗粒（SPIONs）的加入解决了这个问题。SPIONs的结合可以通过磁场梯度实现实时可编程操作。这种融合不仅赋予了水凝胶磁性材料的磁性推进、定位、磁导向、运动和悬浮等能力，而且还引入了新的功能，如对热效应的响应和增强的吸附能力。这篇综述深入探讨了将SPIONs集成到水凝胶中所释放的变革潜力，展示了它们独特的功能增强以及在机器人、精准医疗和废水处理方面的针对性应用。

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引用次数: 0

A noninvasive accelerated quantitative MR technique to distinguish drug resistance in prolactinomas: Early results 一种无创加速定量MR技术鉴别催乳素瘤的耐药性：早期结果

Biomedical Technology

Pub Date : 2025-06-01 DOI: 10.1016/j.bmt.2025.100086

Rong Lu , Tingfang Hwang , Kaibo Tang , Qing Li , Caixia Fu , Ying-Hua Chu , Shangxuan Shi , Tobias Kober , Tom Hibert , Bin Lu , Yiming Li , Yao Lu , Weijun Tang , Lijin Ji

Management of dopamine agonist (DA) resistance in prolactinoma (PRLoma) remains challenging due to the lack of detecting approach and reliable imaging marker. In this cross-sectional study, PRLoma patients were recruited prospectively from a single-center outpatient clinic from May 2024 to September 2024. A noninvasive accelerated quantitative MR technique (GRAPPATINI, GeneRalized Autocalibrating Partially Parallel Acquisitions acceleraTed T2 mappINg) was applied to investigate the correlation between quantitative T2 values and DA resistance. The T2 values of tumors with multiple echo times (TEs) and signal intensity (SI) ratios were analyzed. A total of 30 participants were included, of which 20 were DA-sensitive and 10 were DA-resistant. The T2 values were significantly lower in DA-resistant PRLoma than those in DA-sensitive PRLoma (76.85 ± 29.84 ms vs 135.88 ± 69.86 ms, P = 0.0048), whereas the T2 SI ratio revealed no significant difference. The area under the curve (AUC) of T2 values and T2 SI ratio for distinguishing DA sensitivity were 0.850 and 0.668, respectively. The ratio distributions at different TEs (TE10 ms, TE38 ms, TE42 ms, TE55 ms, and TE90 ms) were significantly different at TE38 ms (P = 0.026) between two groups. The AUC of T2 values and TEs for DA sensitivity in PRLoma were 0.850 (T2map), 0.680 (TE10 ms), 0.730 (TE38 ms), 0.690 (TE42 ms), 0.520 (TE55 ms), and 0.670 (TE90 ms), respectively (P = 0.0107). These findings highlight the translational potential of T2 values as a promising imaging biomarker for evaluating DA resistance in PRLoma.

由于缺乏检测方法和可靠的成像标记，催乳素瘤（proloma）患者多巴胺激动剂（DA）耐药性的管理仍然具有挑战性。在这项横断面研究中，从2024年5月至2024年9月的单中心门诊诊所前瞻性地招募了proma患者。采用无创加速定量MR技术（GRAPPATINI，广义自校准部分平行采集加速T2映射）研究定量T2值与DA电阻之间的相关性。分析肿瘤多次回波时间（TEs）和信号强度（SI）比值的T2值。共纳入30例受试者，其中da敏感20例，da耐药10例。da耐药PRLoma的T2值明显低于da敏感PRLoma(76.85±29.84 ms vs 135.88±69.86 ms, P = 0.0048)，而T2 SI比值无显著差异。T2值和T2 SI比值的曲线下面积（AUC）分别为0.850和0.668。两组在TE10 ms、TE38 ms、TE42 ms、TE55 ms和TE90 ms时的比值分布在TE38 ms时差异有统计学意义（P = 0.026）。前列腺癌DA敏感性的T2值和TEs的AUC分别为0.850 （T2map）、0.680 （TE10 ms）、0.730 （TE38 ms）、0.690 （TE42 ms）、0.520 （TE55 ms）和0.670 (TE90 ms) （P = 0.0107）。这些发现强调了T2值作为评估前列腺增生瘤DA耐药性的有希望的成像生物标志物的转化潜力。

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引用次数: 0

Gαi1 activation induced by short-term hypoxia promotes epidermal cell migration in wound healing through the Akt-mTOR pathway 短期缺氧诱导的Gαi1激活通过Akt-mTOR通路促进表皮细胞在创面愈合中的迁移

Biomedical Technology

Pub Date : 2025-03-05 DOI: 10.1016/j.bmt.2025.100072

Jianghe Zhang , Yan Yan , Jun Wan , Yiming Zhang , Junli Zhou

Hypoxia is commonly observed in the wound microenvironment; however, the specific mechanism by which it affects epidermal cell migration remains unclear. This study aimed to examine the impact of hypoxia on epidermal cell migration and explore the underlying mechanisms involved. The impact of hypoxia (1 % oxygen) on the migration of an immortal keratinocyte cell line, HaCaT, was evaluated using a scratch assay and live cell imaging system. The activation of Gαi1 in HaCaT cells following hypoxia treatment was detected by immunoprecipitation. Possible biological mechanisms were explored through gene microarray assays and bioinformatics methods. Furthermore, the effect of Gαi1 loss-of-function on wound healing was investigated using a rat wound model. Short-term hypoxia significantly enhances HaCaT cell migration and Gαi activation, also observed in rat wound tissue. However, the migration difference between hypoxic and normoxic conditions is negligible after Gαi1 knockdown, highlighting Gαi1's role. Bioinformatics analysis points to the PI3K-Akt pathway as a key mediator, which is confirmed by Akt inhibitor experiments. Additionally, Gαi1 promotes cell migration via the PI3K/Akt/mTORC pathway under hypoxia, and Gαi1 knockdown in rats results in slower wound healing and reduced re-epithelialization. Short-term hypoxia promotes epidermal cell migration through the activation of the Akt-mTOR pathway by Gαi1. Defects in Gαi1 lead to impaired re-epithelialization and delayed wound healing in rats.

缺氧常见于伤口微环境；然而，其影响表皮细胞迁移的具体机制尚不清楚。本研究旨在探讨缺氧对表皮细胞迁移的影响，并探讨其潜在机制。缺氧（1%氧气）对不朽角质形成细胞系HaCaT迁移的影响，使用划痕试验和活细胞成像系统进行评估。免疫沉淀法检测缺氧后HaCaT细胞中Gαi1的活化情况。通过基因微阵列分析和生物信息学方法探讨了可能的生物学机制。此外，采用大鼠创面模型研究了Gαi1功能缺失对创面愈合的影响。短期缺氧可显著增强HaCaT细胞的迁移和g - αi的活化。然而，Gαi1敲低后，低氧和常氧条件下的迁移差异可以忽略不计，这突出了Gαi1的作用。生物信息学分析指出PI3K-Akt通路是关键的介质，Akt抑制剂实验证实了这一点。此外，Gαi1在缺氧条件下通过PI3K/Akt/mTORC途径促进细胞迁移，Gαi1敲低导致大鼠伤口愈合减慢和再上皮化减少。短期缺氧通过g α 1激活Akt-mTOR通路促进表皮细胞迁移。Gαi1缺陷导致大鼠再上皮化受损和伤口愈合延迟。

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引用次数: 0

Breathable functional aerogel dressings facilitate the healing of diabetic wounds 透气功能气凝胶敷料促进糖尿病伤口愈合

Biomedical Technology

Pub Date : 2025-02-21 DOI: 10.1016/j.bmt.2025.100071

Mingyue Liu , Yu Chen , Yu Zhang , Pengzhen Zhuang , Juan Wang

Due to the complexity of the microenvironment and healing process of diabetic wounds, developing wound dressings that offer good biocompatibility, breathability, and promote vascular regeneration is essential but remains a significant challenge. In this study, we prepared loose and porous aerogel wound dressings using electrospinning and freeze-drying methods with natural polymer compounds: gelatin, polylactic acid, and magnesium oxide nanoparticles (MgO) as raw materials. MgO serves as a functional modification component that regulates the wound microenvironment and promotes vascular regeneration by releasing bioactive ions, thereby facilitating wound healing. Additionally, this scaffold provides excellent breathability and exudate management due to its loose porous structure. These advantages enable the aerogel scaffold to effectively promote collagen deposition and neovascularization, accelerating the healing of diabetic infected wounds.

由于糖尿病创面微环境和愈合过程的复杂性，开发具有良好生物相容性、透气性和促进血管再生的创面敷料至关重要，但仍是一个重大挑战。在本研究中，我们以明胶、聚乳酸和氧化镁纳米颗粒（MgO）为原料，采用静电纺丝和冷冻干燥的方法制备了疏松多孔的气凝胶伤口敷料。MgO作为一种功能性修饰成分，通过释放生物活性离子调节创面微环境，促进血管再生，促进创面愈合。此外，由于其松散的多孔结构，这种支架提供了出色的透气性和渗出物管理。这些优点使得气凝胶支架能够有效促进胶原沉积和新生血管，加速糖尿病感染伤口的愈合。

引用次数: 0

Progress of metal-organic frameworks in improving the effect of sonodynamic therapy 金属-有机骨架提高声动力治疗效果的研究进展

Biomedical Technology

Pub Date : 2025-02-14 DOI: 10.1016/j.bmt.2025.100070

Yu Bai , Jine Wang , Ruhui Yan , Yuxian Zhao , Shuang Han , Zhichao Zhang , Yuewu Zhao

Sonodynamic therapy (SDT) is a new non-invasive and precise tumor treatment method, which is a promising anticancer treatment and is becoming a cutting-edge interdisciplinary research field. Based on the research progress of SDT, this paper reviews the latest situation of metal-organic frameworks (MOF) based sonosensitizer in recent years, and introduces the preparation methods and related properties of MOF materials. By describing the many in-depth observations and understandings of MOF-assisted SDT strategies in anticancer applications, we aim to highlight the advantages and improvements of MOF-enhanced SDT and synergistic therapy. The methods to improve the effect of SDT were discussed by exploring the measures of combining hypoxia activating drugs, improving hypoxia microenvironment, accelerating glutathione consumption, and enhancing cavitation effect. Based on the extensive application of MOF materials in SDT and the related technical challenges, this review hopes to bring some enlightenment to improve the therapeutic effect of cancer and promote the development of nanomedicine.

声动力治疗（SDT）是一种新型的无创、精准的肿瘤治疗方法，是一种很有前景的抗癌治疗方法，正在成为一个前沿的跨学科研究领域。基于SDT的研究进展，综述了近年来基于金属有机框架（MOF）的声敏剂的最新研究进展，介绍了MOF材料的制备方法及相关性能。通过描述对mof辅助SDT策略在抗癌应用中的许多深入观察和理解，我们旨在突出mof增强SDT和协同治疗的优势和改进。从联合缺氧激活药物、改善缺氧微环境、加速谷胱甘肽消耗、增强空化效应等方面探讨提高SDT效果的方法。基于MOF材料在SDT中的广泛应用以及相关的技术挑战，本文希望对提高肿瘤治疗效果，促进纳米医学的发展带来一些启示。

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引用次数: 0

Virus-inspired biogenic delivery system for advancing cancer therapy 促进癌症治疗的病毒激发生物传递系统

Biomedical Technology

Pub Date : 2025-02-08 DOI: 10.1016/j.bmt.2025.100069

Di Sun , Hao Liang , Qianwen Mu , Chengchao Chu , Gang Liu , Chao Liu

Virus-inspired particles have been utilized in various applications, including vaccination, gene therapy, drug therapy, and diagnostics. Biogenic delivery systems imitating the natural structure of viruses are regarded as innovative nanoplatforms used to deliver drug compounds to related sites and target cells in organisms. Among them, the components comprised of virus-like particles (VLPs) derive from the proteins or peptides of the viruses; the glycoproteins on their surface exert significant function as specific targeting. Types of assembled glycoproteins and encapsulated drug molecules confer the complexity and varieties of structure, function, and treatment of VLPs. VLPs lack viral virulence, resulting from a viral genetic material deficiency. In vaccine research, Virus-mimic nanovesicles have been effectively verified against cancer via the immunogenicity and the pharmacological effect of drug molecules delivered to mediate an immune response in the body. This review summarizes the research status of virus-inspired drug delivery platforms for cancer therapy utilization.

病毒激发颗粒已被用于各种应用，包括疫苗接种、基因治疗、药物治疗和诊断。模仿病毒自然结构的生物源传递系统被认为是一种创新的纳米平台，用于将药物化合物传递到生物体内的相关部位和靶细胞。其中，由病毒样颗粒（vlp）组成的组分来源于病毒的蛋白质或多肽；其表面的糖蛋白具有特异性靶向作用。组装的糖蛋白和包封的药物分子的类型决定了VLPs的结构、功能和治疗的复杂性和多样性。由于病毒遗传物质缺乏，VLPs缺乏病毒毒力。在疫苗研究中，模拟病毒的纳米囊泡通过药物分子的免疫原性和药理学作用在体内介导免疫反应，已被有效地证实具有抗癌作用。本文综述了病毒启发给药平台在肿瘤治疗中的应用研究现状。

引用次数: 0

Embracing the future: The application of regenerative biomaterials in the spinal disorders 拥抱未来：再生生物材料在脊柱疾病中的应用

Biomedical Technology

Pub Date : 2024-12-25 DOI: 10.1016/j.bmt.2024.100068

Yiwen Xu , Miaojie Fang , Zilong Li , Yucheng Xue , Kelei Wang , Feng Lin , Ning Zhang

Spinal disorders, particularly disc degeneration, vertebral fractures, and loss of spinal stability, have become a major health problem affecting quality of life worldwide. Although conventional treatments such as surgery and conservative therapy provide some relief, these methods often fail to fully address the underlying problems of spinal disorders. In recent years, the application of regenerative biomaterials has provided new ideas and solutions for the repair and regeneration of spinal disorders. This paper provides a systematic review of the application of regenerative biomaterials in the treatment of spinal disorders, including natural materials, synthetic materials and composites. In addition, this paper describes several advanced tissue engineering fabrication techniques, such as 3D printing and bioprinting, which enable regenerative biomaterials to be applied more precisely in the treatment of spinal disorders. This paper also explores the role of regenerative biomaterials in spinal disorders. The aim of this paper is to provide references and new insights for future exploration of the application of regenerative biomaterials in spinal disorders, and to promote research and clinical practice in related fields, with a view to achieving more effective and safer therapeutic strategies for spinal disorders.

脊柱疾病，特别是椎间盘退变、椎体骨折和脊柱稳定性丧失，已成为影响全世界生活质量的主要健康问题。尽管手术和保守疗法等传统治疗方法可以缓解一些症状，但这些方法往往不能完全解决脊柱疾病的潜在问题。近年来，再生生物材料的应用为脊柱疾病的修复和再生提供了新的思路和解决方案。本文系统综述了再生生物材料在脊柱疾病治疗中的应用，包括天然材料、合成材料和复合材料。此外，本文还介绍了几种先进的组织工程制造技术，如3D打印和生物打印，这些技术使再生生物材料能够更精确地应用于脊柱疾病的治疗。本文还探讨了再生生物材料在脊柱疾病中的作用。本文旨在为未来再生生物材料在脊柱疾病中的应用探索提供参考和新的见解，促进相关领域的研究和临床实践，以期获得更有效、更安全的脊柱疾病治疗策略。

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引用次数: 0

Developing biotechnologies in organoids for liver cancer 开发治疗肝癌的有机体生物技术

Biomedical Technology

Pub Date : 2024-11-26 DOI: 10.1016/j.bmt.2024.100067

Yingzhe Hu , Zheng Peng , Mengdi Qiu , Lingling Xue , Haozhen Ren , Xingyu Wu , Xinhua Zhu , Yitao Ding

Organoids, three-dimensional cellular constructs, have revolutionized in vitro culture by replicating the histological and physiological functions of organs, offering a model that closely mimics physiological conditions. Liver cancer presents a significant challenge due to its heterogeneity and the influence of the liver's microenvironment on therapeutic responses. Organoid technology addresses this complexity by simulating the tumor microenvironment in vitro, capturing the heterogeneity of liver cancer, and facilitating personalized treatment approaches. This study explores the integration of organoids in liver cancer research, focusing on genetic and phenotypic fidelity, disease modeling, and drug screening. We discuss the latest advancements in biotechnology, including CRISPR/Cas9, 3D bioprinting, and microfluidics, and their role in personalized medicine. Despite challenges in scalability and variability, organoids offer a promising avenue for liver cancer research and precision oncology, with the potential to transform our understanding and treatment of this disease.

器官组织是一种三维细胞构建体，通过复制器官的组织学和生理功能，提供了一种近似生理条件的模型，从而彻底改变了体外培养。肝癌因其异质性和肝脏微环境对治疗反应的影响而成为一项重大挑战。类器官技术通过在体外模拟肿瘤微环境、捕捉肝癌的异质性和促进个性化治疗方法，解决了这一复杂性。本研究探讨了有机体在肝癌研究中的整合，重点关注遗传和表型保真度、疾病建模和药物筛选。我们讨论了生物技术的最新进展，包括CRISPR/Cas9、三维生物打印和微流控技术，以及它们在个性化医疗中的作用。尽管在可扩展性和可变性方面存在挑战，但器官组织为肝癌研究和精准肿瘤学提供了一个前景广阔的途径，有可能改变我们对这种疾病的理解和治疗。

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引用次数: 0