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Contraceptive activity of 4-(4-hydroxy-3-methyl-hex-5-enyl)-chroman-2,7-diol via inhibiting ovulation in Gonadotropin-primed immature rat model 促性腺激素诱导的未成熟大鼠模型中,4-(4-羟基-3-甲基-己-5-烯基)-铬-2,7-二醇通过抑制排卵的避孕活性
Pub Date : 2014-01-01 DOI: 10.1016/j.biomag.2013.12.001
Ashok Kumar , Sumeet Gullaiya , Vishal Dubey , Ashish Nagar , Varun Singh , Shyam S. Agrawal

Gonadotropin-primed immature rats (GPIR) were used to study the anti-ovulatory effect of 4-(4-hydroxy-3-methyl-hex-5-enyl)-chroman-2,7-diol (chroman derivative) isolated from seeds of Ensete superbum on ovulation. Present results depicted dose-related anti-ovulatory effect of the chroman derivative at 2 mg/kg bw and 4 mg/kg bw. Chroman derivative showed dose-dependent anti-estrogenic activity on MCF-7 cell line. Attenuation of ovulation was associated with a lowered ovarian estrogen and progesterone levels along with reduced levels of some major cytokines, i.e. TNFα, VEGF, IL-6 and IL-1β, indicating the disrupted cytokinine signaling. Unruptured preovulatory follicles were found in all the ovaries extracted from animals treated with chroman derivative. Our findings demonstrated that chroman derivative has potential contraceptive activity through its anti-estrogenic activity which leads to inhibition in ovulation and attenuated ovarian cytokinine signaling.

采用促性腺激素诱导的未成熟大鼠(GPIR)研究了从麻麻种子中分离的4-(4-羟基-3-甲基-己-5-烯基)-铬-2,7-二醇(铬衍生物)对排卵的抑制作用。目前的研究结果显示,在2 mg/kg bw和4 mg/kg bw时,chroman衍生物的抗排卵作用与剂量相关。Chroman衍生物对MCF-7细胞株显示剂量依赖性的抗雌激素活性。排卵减弱与卵巢雌激素和孕激素水平降低以及一些主要细胞因子如TNFα、VEGF、IL-6和IL-1β水平降低有关,表明细胞分裂素信号通路中断。经chroman衍生物处理的动物卵巢均有未破裂的排卵前卵泡。我们的研究结果表明,色素衍生物具有潜在的避孕活性,通过其抗雌激素活性,导致排卵抑制和卵巢细胞分裂素信号减弱。
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引用次数: 2
Application of chitosan and herbal nanocomposites to develop antibacterial medical textile 壳聚糖与草药纳米复合材料在抗菌医用纺织品中的应用
Pub Date : 2014-01-01 DOI: 10.1016/j.biomag.2013.10.007
S. Chandrasekar , S. Vijayakumar , R. Rajendran

Aim

To enhance the efficiency of biological, chemical and physical properties like antibacterial activity, wash durability, air-permeability and biocompatibility, cotton fabric materials were finished with chitosan and herbal nanocomposites.

Methodology

Extracts of Senna auriculata and Achyranthes aspera with chitosan solution was bulk finished on 40 cotton fabrics. To increase the functional properties, chitosan and herbal extract nanocomposites were finished on to another set of fabrics (nanocomposite finishing). Different functional properties were carried out for both the sets of fabrics and comparatively analyzed. Antibacterial activity was carried out using standard EN ISO 20645 method and durability of finished fabrics was done using a standard AATCC–124 test method. Physical properties like tensile strength, abrasion resistance and air-permeability were analyzed using the standards, ASTM D 5035-2006, AATCC 119-2004 and ASTM D 737-1996 respectively. Biocompatible properties of the finished fabric were determined by HET-CAM to ensure that material do not cause irritation points on skin surface.

Results

Antibacterial activity of nanocomposite finished fabrics showed more inhibitory zones of 31 mm for E. coli and 29 mm for S. aureus when compared to bulk finished fabrics. Nanocomposite finished fabrics showed good durable properties and physical properties than bulk finished fabrics. Bulk and nanocomposite finished fabrics showed good biocompatible properties when analyzed using a standard HET-CAM test.

Conclusion

The study concludes that, nanocomposites could provide better functional properties than the bulk finished fabrics. The nano-sized particles in the composites was considered significant for its functional applications in hospital based fabrics to prevent the transmission of nosocomial infections.

目的为提高棉织物的生物、化学和物理性能,如抗菌活性、洗涤耐久性、透气性和生物相容性,采用壳聚糖和草药纳米复合材料对棉织物材料进行整理。方法采用壳聚糖溶液对木耳番泻和牛膝的提取液进行批量整理。为了提高功能性能,将壳聚糖和草药提取物纳米复合材料整理在另一组织物上(纳米复合整理)。对两组织物的不同功能性能进行了对比分析。抗菌活性采用标准EN ISO 20645测试方法,织物耐久性采用标准AATCC-124测试方法。采用ASTM D 5035-2006、AATCC 119-2004和ASTM D 737-1996分别对拉伸强度、耐磨性和透气性等物理性能进行了分析。成品织物的生物相容性由ht - cam测定,以确保材料不会对皮肤表面造成刺激点。结果纳米复合织物对大肠杆菌和金黄色葡萄球菌的抑制区分别为31 mm和29 mm。纳米复合整理织物具有较好的耐久性能和物理性能。当使用标准的高温热成像(ht - cam)测试分析时,散装和纳米复合织物显示出良好的生物相容性。结论纳米复合材料具有较好的功能性。该复合材料中的纳米级颗粒被认为是显著的可用于医院基织物的功能应用,以防止医院感染的传播。
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引用次数: 36
Poly lactic acid (PLA) nanoparticles sustain the cytotoxic action of temozolomide in C6 Glioma cells 聚乳酸(PLA)纳米颗粒维持替莫唑胺对C6胶质瘤细胞的细胞毒性作用
Pub Date : 2013-10-01 DOI: 10.1016/j.biomag.2013.08.003
Darshana S. Jain , Rajani B. Athawale , Amrita N. Bajaj , Shruti S. Shrikhande , Peeyush N. Goel , Yuvraj Nikam , Rajiv P. Gude

Targeting brain tumor largely depends on the chemotherapeutic agent uptake by the cancerous cells. The uptake further depends on its dosage form, route of administration and surface modifications. In the present study, temozolomide (TMZ) loaded into poly lactic acid (PLA) nanoparticles was employed to evaluate the efficacy against C6 glioma cells. Solvent evaporation method was used to fabricate PLA nanoparticles. Particle size determination, zeta potential and diffusion studies were performed for the plain drug and drug-loaded nanoparticulate delivery system. The functional assessment was performed using in vitro studies, such as MTT, clonogenic and wound scratch assays respectively. The cellular uptake of formulated nanoparticles was investigated using flow cytometry and confocal microscopy. The results indicate that the action of TMZ against C6 glioma is sustained when loaded into nanoparticles. This observation is of clinical significance, as it would avoid repeated administration of the drug as well as minimising the side effects and toxicity.

脑肿瘤的靶向治疗在很大程度上取决于肿瘤细胞对化疗药物的摄取。吸收进一步取决于其剂型、给药途径和表面修饰。本研究采用替莫唑胺(TMZ)负载聚乳酸(PLA)纳米颗粒来评价其对C6胶质瘤细胞的作用。采用溶剂蒸发法制备聚乳酸纳米颗粒。对普通药物和载药纳米颗粒递送系统进行了粒径测定、zeta电位和扩散研究。功能评估采用体外研究,如MTT,克隆和伤口划伤试验分别进行。利用流式细胞术和共聚焦显微镜研究了配方纳米颗粒的细胞摄取。结果表明,TMZ对C6胶质瘤的作用是持续的。这一观察结果具有临床意义,因为它可以避免重复给药,并将副作用和毒性降到最低。
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引用次数: 21
Antinociceptive effect of Phyllanthus fraternus extract in complete Freund's adjuvant induced chronic pain in mice 余甘子提取物对完全弗氏佐剂诱导的小鼠慢性疼痛的抗伤性作用
Pub Date : 2013-10-01 DOI: 10.1016/j.biomag.2013.09.001
Atul R. Chopade, F.J. Sayyad

Objective

To evaluate the effects of Phyllanthus fraternus (PF) extract on chronic inflammatory pain.

Method

Complete Freund's adjuvant (CFA) induced inflammatory pain model was used to investigate the effects of (PF) extract on chronic inflammatory hypersensitivity in adult Swiss albino mice. Twenty-four hours after CFA injection, we used Von Frey filaments and acetone-evoked cooling to evaluate tactile and thermal hypersensitivity, respectively. The paw edema was measured with the help of a digital plethysmometer.

Results

Single intraperitoneal injections of PF extract (200 & 400 mg/kg) administered 30 minutes before testing reduced CFA-induced mechanical hypersensitivity. Similarly, PF reduced acetone-evoked hypersensitivity up to 4 hours after treatment. Compared with vehicle, PF produced a marked reduction in CFA-induced paw edema. Multiple treatments with PF extracts given chronically (twice a day from 1st to 5th day and from 9th to 10th day) significantly reduced mechanical hypersensitivity induced by CFA. This multi-dose strategy did not cause tolerance.

Conclusion

The present study reveals the antinociceptive activity of PF in chronic inflammatory hyperalgesia and also indicates that it might be potentially effective in management of the persistent pain.

目的评价余甘子提取物对慢性炎症性疼痛的治疗作用。方法采用完全弗氏佐剂(CFA)诱导的炎症性疼痛模型,研究(PF)提取物对成年瑞士白化小鼠慢性炎症超敏反应的影响。注射CFA 24小时后,我们分别用Von Frey纤维和丙酮诱发冷却来评估触觉和热超敏反应。脚掌水肿在数字容积计的帮助下测量。结果单次腹腔注射PF提取物(200 &试验前30分钟给药400mg /kg,可降低cfa引起的机械超敏反应。同样,PF可在治疗后4小时内降低丙酮诱发的超敏反应。与对照组相比,PF显著减少了cfa诱导的足部水肿。长期给予PF提取物多次处理(第1 ~ 5天和第9 ~ 10天每天两次)可显著降低CFA引起的机械超敏反应。这种多剂量策略没有引起耐受性。结论本研究揭示了PF在慢性炎症性痛觉过敏中的抗痛觉活性,并提示其可能对治疗持续性疼痛有效。
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引用次数: 10
Modulation of insulin transport by D-glucose in alveolar epithelial cells d -葡萄糖在肺泡上皮细胞中对胰岛素转运的调节
Pub Date : 2013-10-01 DOI: 10.1016/j.biomag.2013.09.003
Keisuke Oda , Ryoko Yumoto , Junya Nagai , Mikihisa Takano

D-glucose concentration in alveolar lining fluid is maintained much lower than that in the plasma, but is elevated by hyperglycemia. In the present study, we examined insulin uptake in A549 human alveolar epithelial cells; especially focusing on the effect of D-glucose on the uptake. Time- and temperature-dependence were observed in FITC-insulin uptake in A549 cells in the presence of D-glucose. Confocal laser scanning microscopic analysis showed the punctate localization of FITC-insulin in the cells. These results indicated that FITC-insulin was taken up by the cells through an endocytotic pathway. FITC-insulin uptake was increased 2–4 fold by extracellular D-glucose, but glucose analogs such as 3-O-methyl-D-glucose and 2-deoxy-D-glucose did not show any enhancement effect. Moreover, FITC-albumin and FD-4 uptake were not affected by D-glucose. Intracellular ATP amount was not affected by D-glucose during the incubation. Phloretin, a facilitative glucose transporter inhibitor, significantly inhibited D-glucose uptake as well as D-glucose-stimulated FITC-insulin uptake. Taken together, D-glucose may regulate the insulin uptake after being taken up by A549 cells, through the mechanism that is not directly related to ATP production. The effect of D-glucose on insulin absorption was also examined in in-vivo pulmonary administration study in rats, and co-administration of D-glucose potentiated the hypoglycemic action of insulin. These findings suggest that endocytosis is involved in insulin uptake, and D-glucose specifically enhances the endocytosis of insulin in alveolar epithelial cells. D-glucose concentration in alveolar lining fluid may play an important role in the absorption of insulin from the lung.

肺泡衬液中的d -葡萄糖浓度维持在远低于血浆中的水平,但会因高血糖而升高。在本研究中,我们检测了A549人肺泡上皮细胞的胰岛素摄取;尤其关注d -葡萄糖对摄取的影响。在d -葡萄糖存在的情况下,A549细胞对fitc -胰岛素的摄取具有时间和温度依赖性。共聚焦激光扫描显微镜分析显示fitc -胰岛素在细胞中的点状定位。这些结果表明fitc -胰岛素通过内吞途径被细胞吸收。细胞外d -葡萄糖使fitc -胰岛素摄取增加2-4倍,而葡萄糖类似物如3- o -甲基-d -葡萄糖和2-脱氧-d -葡萄糖没有任何增强作用。此外,fitc -白蛋白和FD-4摄取不受d -葡萄糖的影响。在孵育期间,细胞内ATP的数量不受d -葡萄糖的影响。根皮素是一种促进性葡萄糖转运蛋白抑制剂,可显著抑制d -葡萄糖摄取以及d -葡萄糖刺激的fitc -胰岛素摄取。综上所述,d -葡萄糖被A549细胞摄取后,可能通过与ATP产生无直接关系的机制调节胰岛素的摄取。d -葡萄糖对胰岛素吸收的影响也在大鼠体内肺给药研究中得到验证,同时给药d -葡萄糖可增强胰岛素的降糖作用。这些发现表明,内吞作用参与胰岛素摄取,而d -葡萄糖特异性地增强了肺泡上皮细胞对胰岛素的内吞作用。肺泡衬液中d -葡萄糖浓度可能在肺吸收胰岛素中起重要作用。
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引用次数: 0
Chemopreventive and therapeutic efficacy of Salsola inermis extract against N-nitrosodiethylamine-initiated and phenobarbital-promoted hepatocellular carcinogenesis in Wistar rats 荆芥提取物对n -亚硝基二乙胺引发和苯巴比妥促进的Wistar大鼠肝细胞癌的化学预防和治疗作用
Pub Date : 2013-10-01 DOI: 10.1016/j.biomag.2013.08.002
Samir F. Zohny , Ahlam H. Mahmoud , Ibrahim H. Borai , Faten S. Bayoumi , Eman Eissa

Hepatocellular carcinoma is one of the most common cancers worldwide and is known to be resistant to conventional chemotherapy. Therefore, we aimed to assess the Salsola inermis extract as a novel chemopreventive and/or therapeutic agent against N-nitrosodiethylamine (DNE)/phenobarbital (PB)-induced hepatocarcinogenesis in rats. Adult male Wistar albino rats were divided into five groups: group 1 rats were served as normal controls; group 2 rats were injected intraperitoneally with S. inermis extract (100 mg/kg body weight/day) for 20 weeks; group 3 rats were subjected to two-phase hepatocarcinogenic regimen (initiation of hepatocarcinogenesis was performed by a single intraperitoneal injection of DEN at a dose of 200 mg/kg body weight, 2 weeks later, the carcinogenic effect was promoted by supplementation of rats with 0.05% PB for 16 weeks); group 4 rats were injected intraperitoneally with S. inermis extract 2 weeks prior to the injection of DEN, the daily injection of S. inermis extract was then continued for 18 weeks along with two-phase hepatocarcinogenic regimen (chemoprevention group); and group 5 rats were subjected to the two-phase hepatocarcinogenic regimen, and then, the animals were injected intraperitoneally with S. inermis extract for 4 weeks (treatment group). The activities of serum liver enzymes and levels of total bilirubin, conjugated bilirubin, α-fetoprotein, vascular endothelial growth factor (VEGF) and soluble intercellular adhesion molecule-1 (sICAM-1) in serum were decreased in chemopreventive and treated rats compared with DEN/PB-administered rats. Interestingly, the serum levels of total protein and albumin were normalized in chemopreventive and treated rats. Moreover, the majority of chemopreventive and treated rats showed an almost normal histological pattern of liver. In conclusion, S. inermis extract possessed chemopreventive and therapeutic activities against hepatocarcinogenesis in rats partially through the inhibition of VEGF and sICAM-1.

肝细胞癌是世界范围内最常见的癌症之一,并且已知对常规化疗具有耐药性。因此,我们的目的是评估Salsola inermis提取物作为一种新的化学预防和/或治疗剂对n -亚硝基二乙胺(DNE)/苯巴比妥(PB)诱导的大鼠肝癌的发生。将成年雄性Wistar白化大鼠分为5组:1组大鼠为正常对照组;2组大鼠腹腔注射金针叶提取物(100 mg/kg体重/天),连续20周;3组大鼠采用两期肝癌方案(以200 mg/kg体重单次腹腔注射DEN开始肝癌形成,2周后再补充0.05% PB,持续16周促进其致癌作用);4组大鼠在注射DEN前2周腹腔注射金针叶提取物,每日注射金针叶提取物,连续18周,并给予两期肝癌治疗方案(化学预防组);第5组大鼠采用两期肝癌治疗方案,然后腹腔注射金针叶提取物4周(治疗组)。与DEN/ pb组相比,化学预防组大鼠血清肝酶活性降低,血清总胆红素、结合胆红素、α-胎蛋白、血管内皮生长因子(VEGF)和可溶性细胞间粘附分子-1 (sICAM-1)水平降低。有趣的是,化学预防和治疗大鼠的血清总蛋白和白蛋白水平恢复正常。此外,大多数化学预防和治疗大鼠的肝脏组织学模式几乎正常。综上所述,荆芥提取物部分通过抑制VEGF和sICAM-1具有化学防治大鼠肝癌的作用。
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引用次数: 2
Antidiabetic and antihyperlipidemic effect of p-hydroxycinnamic acid on streptozotocin-induced diabetic Wistar rats 对羟基肉桂酸对链脲佐菌素诱导的糖尿病Wistar大鼠的降糖降脂作用
Pub Date : 2013-10-01 DOI: 10.1016/j.biomag.2013.09.004
Sagadevan Ambika , Ramalingam Saravanan , Kumarasamy Thirumavalavan

The objective of this study was to examine the hypothesis that intake of p-hydroxycinnamic acid (p-HCA) regulates blood glucose levels and hypolipidemic responses in rats with diabetes mellitus induced by injection of streptozotocin (STZ). The diabetic rats exhibited lower levels of body weight, insulin, hemoglobin (Hb), high-density lipoprotein (HDL) and higher levels of glucose, lipid profiles, lipid peroxidation markers in blood hepatic and renal tissues as compared with normal rats. In addition, diabetic rats showed decreased levels of superoxide dismutase (SOD) and catalase (CAT). Oral administration of p-HCA at the doses of 10, 20 and 40 mg/kg BW. was given once daily started after the rise of blood glucose for 45 days. Our result demonstrated that p-HCA administration could be lowered blood glucose, glycosylated hemoglobin, lipid profiles and significant increase in plasma insulin, hemogloblin, and HDL. In addition exert protective action against oxidative markers, TBARS and lipid hydroperoxides and improves antioxidant. The overall results suggest that p-HCA possesses potential hyperglycemic, hyperlipidemic and antioxidant activity in STZ-induced diabetic rats.

本研究旨在探讨对羟基肉桂酸(p-HCA)对注射链脲佐菌素(STZ)诱导的糖尿病大鼠血糖水平和低血脂反应的调节作用。与正常大鼠相比,糖尿病大鼠表现出较低的体重、胰岛素、血红蛋白(Hb)、高密度脂蛋白(HDL)水平和较高的葡萄糖、脂质谱、血液中肝脏和肾脏组织中的脂质过氧化标志物水平。此外,糖尿病大鼠超氧化物歧化酶(SOD)和过氧化氢酶(CAT)水平降低。口服10、20、40 mg/kg BW的p-HCA。每日1次,血糖升高后开始服用,持续45天。我们的研究结果表明,p-HCA可以降低血糖、糖化血红蛋白、血脂,并显著增加血浆胰岛素、血红蛋白和高密度脂蛋白。对氧化标志物、TBARS和脂质氢过氧化物均有保护作用,提高抗氧化能力。综上所述,p-HCA对stz诱导的糖尿病大鼠具有潜在的高血糖、高血脂和抗氧化活性。
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引用次数: 29
Amelioration of cardiac hypertrophy induced by abdominal aortic banding in ferulic acid treated rats 阿魏酸对大鼠腹主动脉束带所致心肌肥厚的改善作用
Pub Date : 2013-10-01 DOI: 10.1016/j.biomag.2013.08.001
Urmila Aswar, Umesh Mahajan, Geetanjali Nerurkar, Manoj Aswar

The objective of the present study was to investigate the beneficial effects of treatment of the abundant phytochemical Ferulic acid (FA), alone or in combination with angiotensin II receptor antagonist telmisartan on pressure overload–induced cardiac hypertrophy in rats. Albino Wistar rats were divided into 6 groups: Sham, abdominal aorta constriction (AAB) and three FA (20 mg/kg) treatment groups given as a pretreatment or post-treatment or in combination with telmisartan (Tel; 10 mg/kg). The standard drug used was Tel (10 mg/kg). The pretreatment was given for 35 days while the other treatments were started from the 29th day of surgery for a period of 7 days orally. After recording the exercise testing and hemodynamic parameters, animals were sacrificed for biochemical and histopathological studies. The AAB group had a significant increase in left ventricular weight (LVW) (p < 0.001), elevated blood pressure (p < 0.001), a significant increase in reactive oxygen species (ROS) (p < 0.001) and serum creatinine kinase-MB (CK-MB) (p < 0.001), significantly lowered glutathione concentration and superoxide dismutase (SOD) (p < 0.001). Pretreatment with FA alone or post-treatment of FA in combination with telmisartan attenuated the rise in LVW and blood pressure (p < 0.001). It eliminated the ROS significantly (p < 0.01), reduced CK-MB, raised the glutathione and SOD (p < 0.001). These findings confirm the protective role of pretreatment of FA and post-treatment of FA with telmisartan in pressure overload–induced cardiac hypertrophy mediated through its integrated effect of potent antioxidative, anti-hypertensive and anti-inflammatory activity.

本研究的目的是探讨丰富的植物化学物质阿魏酸(FA)单独或与血管紧张素II受体拮抗剂替米沙坦联合治疗大鼠压力超载诱导的心脏肥厚的有益作用。将白化Wistar大鼠分为6组:假手术组、腹主动脉缩窄组(AAB)和3个FA (20 mg/kg)治疗组,分别作为治疗前、治疗后或联合替米沙坦(Tel;10毫克/公斤)。标准用药为Tel (10 mg/kg)。预处理35 d,其余治疗从手术第29天开始口服,为期7 d。记录运动试验和血流动力学参数后,处死动物进行生化和组织病理学研究。AAB组左室重量(LVW)显著升高(p <0.001),血压升高(p <0.001),活性氧(ROS)显著增加(p <0.001)和血清肌酐激酶- mb (CK-MB) (p <0.001),显著降低谷胱甘肽浓度和超氧化物歧化酶(SOD) (p <0.001)。单用FA预处理或后用FA联合替米沙坦可减轻LVW和血压升高(p <0.001)。它能显著消除ROS (p <0.01),降低CK-MB,升高谷胱甘肽和SOD (p <0.001)。这些发现证实了FA预处理和替米沙坦后处理FA通过其强大的抗氧化、抗高血压和抗炎活性的综合作用介导的压力超负荷性心脏肥厚的保护作用。
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引用次数: 7
Effect of Cyperus rotundus tubers ethanolic extract on learning and memory in animal model of Alzheimer 香附乙醇提取物对阿尔茨海默病动物模型学习记忆的影响
Pub Date : 2013-10-01 DOI: 10.1016/j.biomag.2013.08.006
Zahra Rabiei , Mohammadreza Hojjati , Mahmoud Rafieian-Kopaeia , Zahra Alibabaei

Alzheimer's disease (AD) is a degenerative disorder that leads to progressive cognitive decline. The memory dysfunction of AD has been associated with a cortical cholinergic deficiency and loss of cholinergic neurons of the nucleus basalis of Meynert (NBM). This study investigated the effect of Cyperus rotundus tubers ethanolic extract on learning and memory in the rat model of Alzheimer's and intact rats. In this experimental study, 49 male Wistar rats were divided randomly into seven groups. Wistar rats received bilateral electric lesions of the NBM. The NBM-lesioned rats received Cyperus rotundus tubers ethanolic extract (100 and 200 mg/kg; ip) for 15 days while the control and sham groups received distilled water. Intact rats received extract without any surgery. Passive avoidance learning and memory performance was assessed using passive avoidance paradigm, and Morris water maze test used for spatial learning and memory evaluation. In shuttle box test, the initial latency significantly decreased in Alzh+ CRT (NBM-lesioned rats that received extract) groups. Step through latency significantly increased in Alzh+ CRT groups. In Morris water maze task, in the probe trial, Alzh+ CRT groups demonstrated a significant preference for the quadrant in which the platform was located on the preceding day. The decrease in the escape latency improved significantly in Alzh+ CRT groups when compared with Alzh (NBM-lesioned rats) group. The results suggest that Cyperus rotundus tubers ethanolic extract has some repairing effects on the memory and behavioral disorders produced by lesioning of the NBM in rats.

阿尔茨海默病(AD)是一种导致进行性认知能力下降的退行性疾病。阿尔茨海默病的记忆功能障碍与皮层胆碱能缺乏和Meynert基底核胆碱能神经元的丧失有关。本研究探讨了香附乙醇提取物对阿尔茨海默病大鼠模型和正常大鼠学习记忆的影响。本实验选取雄性Wistar大鼠49只,随机分为7组。Wistar大鼠双侧脑基底电损伤。nbm损伤大鼠分别给予香附乙醇提取物100、200 mg/kg;对照组和假手术组接受蒸馏水治疗。未做任何手术的大鼠接受提取物。采用被动回避范式评价被动回避学习记忆能力,Morris水迷宫测验评价空间学习记忆能力。穿梭箱试验中,Alzh+ CRT(接受提取物的nbm损伤大鼠)组的初始潜伏期明显降低。Alzh+ CRT组步过潜伏期明显增加。在Morris水迷宫任务中,在探针试验中,Alzh+ CRT组表现出对前一天平台所在象限的显著偏好。与Alzh (nbm损伤大鼠)组相比,Alzh+ CRT组逃避潜伏期的减少明显改善。结果提示,香附乙醇提取物对大鼠脑基底膜损伤所致的记忆和行为障碍具有一定的修复作用。
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引用次数: 48
Protection against aluminium neurotoxicity: A repertoire of lettuce antioxidants 对铝神经毒性的保护:生菜抗氧化剂的曲目
Pub Date : 2013-10-01 DOI: 10.1016/j.biomag.2013.08.004
S.N. Harsha, K.R. Anilakumar

Exposure to aluminium, though toxic in various ways is unavoidable owing to the abundant presence and use of aluminium in everyday living. Also, the fact that aluminium toxicity can lead to serious, life-threatening complications provides compelling reasons to find novel and effective therapeutic interventions. A distinct feature of aluminium toxicity is its ability to create pro-oxidant environment. The present study investigates the effectiveness of the anti-oxidant property of Lactuca sativa as a neuroprotective agent against aluminium induced neurotoxicity. The lyophilized hydro-ethanol extract of Lactuca sativa (LS) was reconstituted in water and administered orally at doses of 200 and 400 mg/kg body weight to mice for a period of 30 days. Aluminium chloride was injected intraperitonially at a conc. of 10 mg/kg body weight. Behavioral tests viz., novel object recognition and open field test were performed to assess the exploratory behavior of mice. Biochemical parameters including lipid peroxidation, nitrite levels, ROS, LDH, glutathione, catalase and acetylcholinesterase activity was assayed in the plasma and brain homogenates. Administration of the plant extract significantly improved the exploratory behavior of mice and also attenuated oxidative stress and decreased the acetylcholinesterase activity. Hence, we report a significant recoupment in the blood and biochemical parameters with the treatment of Lactuca sativa extract against aluminium chloride induced neurotoxicity.

由于铝在日常生活中的大量存在和使用,虽然铝以各种方式有毒,但接触铝是不可避免的。此外,铝毒性可导致严重的、危及生命的并发症,这一事实为寻找新颖有效的治疗干预措施提供了令人信服的理由。铝毒性的一个显著特征是它能够创造促氧化环境。本研究探讨了苜蓿作为一种神经保护剂对铝诱导的神经毒性的抗氧化作用。将lacuca sativa (LS)冻干水乙醇提取物(hydro-ethanol extract)复水,按200和400 mg/kg体重的剂量口服给药30天。腹腔注射氯化铝。每公斤体重10毫克。通过行为测试,即新物体识别和开放场测试来评估小鼠的探索行为。测定血浆和脑匀浆中脂质过氧化、亚硝酸盐水平、ROS、LDH、谷胱甘肽、过氧化氢酶和乙酰胆碱酯酶活性等生化指标。植物提取物能显著改善小鼠的探索行为,减轻氧化应激,降低乙酰胆碱酯酶活性。因此,我们报道了油菜提取物对氯化铝诱导的神经毒性在血液和生化参数上的显著补偿。
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引用次数: 11
期刊
Biomedicine & Aging Pathology
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