Pub Date : 2019-11-20DOI: 10.3760/CMA.J.ISSN.0254-1416.2019.11.024
Tuvshin Bao, Lingxia Niu, Bing-qing Nie, P. Gui, Qiang Xu, Leiming Xia, Yun Lin, S. Yao
Objective To evaluate the effects of different modes of anesthesia on maternal and neonatal outcomes in puerperas with placenta previa and accreta undergoing cesarean section for second birth. Methods The trials involving puerperas with a singleton fetus, aged ≥20 yr, at ≥28 weeks of gestation, with placenta previa and accreta, undergoing caesarean section for second birth, retrospectively reviewed from January 2015 to May 2017 in our hospital, were selected.Puerperas were divided into epidural anesthesia group (group E) and general anesthesia group (group G) according to the mode of anesthesia.Maternal outcomes included incision-delivery time, operating time, postpartum hemorrhage and intraoperative hypotension, intraoperative blood loss, volume of blood transfused, volume of crystalloid solution and colloid solution infused, uterine contractions used≥2 types, implementation of surgical hemostatic measures, hysterectomy, postpartum Hb, transfer to intensive care unit after operation, and length of hospital stay.Neonatal outcomes included premature, Apgar score at 1 and 5 min after birth, transfer to pediatrics unit and perinatal death. Results A total of 269 pueroeras were enrolled in the study, and among the 269 pueroeras, there were 219 cases of elective repeat cesarean section (ERCS) and 50 cases of cesarean section after vaginal birth (CAVB). Pueroeras of ERCS: Compared with G group, the emergency rate was significantly decreased, the prenatal Hb concentration was increased, the operating time and length of hospital stay were shortened, and the intraoperative blood loss, volume of blood transfused, volume of crystalloid solution and colloid solution infused, incidence of postpartum hemorrhage, implementing rate of surgical hemostatic measures, incidence of hysterectomy, postpartum Hb, and rate of transfer to intensive care unit after operation were decreased in E group (P<0.05). Pueroeras of CAVB: Compared with G group, the incidence of postpartum hemorrhage and implementing rate of surgical hemostatic measures were significantly decreased, and the rate of Apgar score≤7 at 1 and 5 min after birth and rate of transfer to pediatrics unit were decreased in E group (P<0.05). Conclusion Compared with general anesthesia, epidural anesthesia has less effect on maternal and infant outcomes and should be preferred for this type of puerperas with placenta praevia and accreta undergoing cesarean section for second birth. Key words: Cesarean section; Anesthesia, general; Anesthesia, epidural; Placenta previa
{"title":"Effects of different modes of anesthesia on maternal and neonatal outcomes in puerperas with placenta previa and accreta undergoing cesarean section for second birth","authors":"Tuvshin Bao, Lingxia Niu, Bing-qing Nie, P. Gui, Qiang Xu, Leiming Xia, Yun Lin, S. Yao","doi":"10.3760/CMA.J.ISSN.0254-1416.2019.11.024","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1416.2019.11.024","url":null,"abstract":"Objective \u0000To evaluate the effects of different modes of anesthesia on maternal and neonatal outcomes in puerperas with placenta previa and accreta undergoing cesarean section for second birth. \u0000 \u0000 \u0000Methods \u0000The trials involving puerperas with a singleton fetus, aged ≥20 yr, at ≥28 weeks of gestation, with placenta previa and accreta, undergoing caesarean section for second birth, retrospectively reviewed from January 2015 to May 2017 in our hospital, were selected.Puerperas were divided into epidural anesthesia group (group E) and general anesthesia group (group G) according to the mode of anesthesia.Maternal outcomes included incision-delivery time, operating time, postpartum hemorrhage and intraoperative hypotension, intraoperative blood loss, volume of blood transfused, volume of crystalloid solution and colloid solution infused, uterine contractions used≥2 types, implementation of surgical hemostatic measures, hysterectomy, postpartum Hb, transfer to intensive care unit after operation, and length of hospital stay.Neonatal outcomes included premature, Apgar score at 1 and 5 min after birth, transfer to pediatrics unit and perinatal death. \u0000 \u0000 \u0000Results \u0000A total of 269 pueroeras were enrolled in the study, and among the 269 pueroeras, there were 219 cases of elective repeat cesarean section (ERCS) and 50 cases of cesarean section after vaginal birth (CAVB). Pueroeras of ERCS: Compared with G group, the emergency rate was significantly decreased, the prenatal Hb concentration was increased, the operating time and length of hospital stay were shortened, and the intraoperative blood loss, volume of blood transfused, volume of crystalloid solution and colloid solution infused, incidence of postpartum hemorrhage, implementing rate of surgical hemostatic measures, incidence of hysterectomy, postpartum Hb, and rate of transfer to intensive care unit after operation were decreased in E group (P<0.05). Pueroeras of CAVB: Compared with G group, the incidence of postpartum hemorrhage and implementing rate of surgical hemostatic measures were significantly decreased, and the rate of Apgar score≤7 at 1 and 5 min after birth and rate of transfer to pediatrics unit were decreased in E group (P<0.05). \u0000 \u0000 \u0000Conclusion \u0000Compared with general anesthesia, epidural anesthesia has less effect on maternal and infant outcomes and should be preferred for this type of puerperas with placenta praevia and accreta undergoing cesarean section for second birth. \u0000 \u0000 \u0000Key words: \u0000Cesarean section; Anesthesia, general; Anesthesia, epidural; Placenta previa","PeriodicalId":10053,"journal":{"name":"中华麻醉学杂志","volume":"39 1","pages":"1367-1370"},"PeriodicalIF":0.0,"publicationDate":"2019-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43004267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-20DOI: 10.3760/CMA.J.ISSN.0254-1416.2019.11.007
Ge Xu, Man Yang, Yang Yu, Yonghao Yu
Objective To evaluate the effect of coenzyme Q10 on the expression of apolipoprotein E (ApoE) in brain damage induced by multiple exposure to sevoflurane in mice. Methods Seventy-two SPF female C57BL/6J mice, aged 6 days, weighing 3-5 g, were divided into 4 groups (n=18 each) according to a random number table method: corn oil group (CO), sevoflurane plus corn oil group (SO group), coenzyme Q10 group (CQ group), and sevoflurane plus coenzyme Q10 group (SQ group). Mice were placed in a closed resin box with an inlet and outlet, 3% sevoflurane plus 60% oxygen plus 37% air was continuously delivered to the box at 2 h/d for 3 consecutive days in SO group and SQ group.The mice were placed in the same resin box, and 60% oxygen plus 40% air was delivered in CO group and CQ group.Corn oil 100 μl was intraperitoneally injected, and 30 min later oxygen and sevoflurane were inhaled in CO group and SO group, respectively.CoQ10 (dissolved in corn oil, concentration 1.5 μg/μl) 50 μg/g was intraperitoneally injected, and 30 min later oxygen and sevoflurane were inhaled in CQ group and SQ group, respectively.Eight mice were sacrificed after emerging from anesthesia, and hippocampi were removed for determination of the expression of ApoE, Tau protein, AT8 and PHF1 (by Western blot). The rest 10 mice were used for Morris Water Maze test to evaluate the cognitive function on 22 days after establishing the model. Results Compared with CO group, the expression of ApoE, AT8 and PHF1 was significantly up-regulated, the escape latency was prolonged, and the number of crossing the platform was decreased in SO group (P 0.05). Conclusion The mechanism by which coenzyme Q10 reduces brain damage induced by multiple exposure to sevoflurane is related to inhibiting the up-regulation of ApoE expression in hippocampus of mice. Key words: Coenzyme Q10; Anesthetics, Inhalation; Cognitive impairment; Apolipoprotein E
{"title":"Effect of coenzyme CoQ10 on expression of apolipoprotein E in brain damage induced by multiple exposure to sevoflurane in mice","authors":"Ge Xu, Man Yang, Yang Yu, Yonghao Yu","doi":"10.3760/CMA.J.ISSN.0254-1416.2019.11.007","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1416.2019.11.007","url":null,"abstract":"Objective \u0000To evaluate the effect of coenzyme Q10 on the expression of apolipoprotein E (ApoE) in brain damage induced by multiple exposure to sevoflurane in mice. \u0000 \u0000 \u0000Methods \u0000Seventy-two SPF female C57BL/6J mice, aged 6 days, weighing 3-5 g, were divided into 4 groups (n=18 each) according to a random number table method: corn oil group (CO), sevoflurane plus corn oil group (SO group), coenzyme Q10 group (CQ group), and sevoflurane plus coenzyme Q10 group (SQ group). Mice were placed in a closed resin box with an inlet and outlet, 3% sevoflurane plus 60% oxygen plus 37% air was continuously delivered to the box at 2 h/d for 3 consecutive days in SO group and SQ group.The mice were placed in the same resin box, and 60% oxygen plus 40% air was delivered in CO group and CQ group.Corn oil 100 μl was intraperitoneally injected, and 30 min later oxygen and sevoflurane were inhaled in CO group and SO group, respectively.CoQ10 (dissolved in corn oil, concentration 1.5 μg/μl) 50 μg/g was intraperitoneally injected, and 30 min later oxygen and sevoflurane were inhaled in CQ group and SQ group, respectively.Eight mice were sacrificed after emerging from anesthesia, and hippocampi were removed for determination of the expression of ApoE, Tau protein, AT8 and PHF1 (by Western blot). The rest 10 mice were used for Morris Water Maze test to evaluate the cognitive function on 22 days after establishing the model. \u0000 \u0000 \u0000Results \u0000Compared with CO group, the expression of ApoE, AT8 and PHF1 was significantly up-regulated, the escape latency was prolonged, and the number of crossing the platform was decreased in SO group (P 0.05). \u0000 \u0000 \u0000Conclusion \u0000The mechanism by which coenzyme Q10 reduces brain damage induced by multiple exposure to sevoflurane is related to inhibiting the up-regulation of ApoE expression in hippocampus of mice. \u0000 \u0000 \u0000Key words: \u0000Coenzyme Q10; Anesthetics, Inhalation; Cognitive impairment; Apolipoprotein E","PeriodicalId":10053,"journal":{"name":"中华麻醉学杂志","volume":"39 1","pages":"1302-1305"},"PeriodicalIF":0.0,"publicationDate":"2019-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41934006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective To evaluate the role of hippocampal CD200 receptor 1 (CD200R1) in perioperative neurocognitive disorders (PND) in mice. Methods Sixty clean-grade male C57BL/6 mice, aged 9-10 months, weighing 32-38 g, were used in the study.The experiment was performed in two parts.Experiment Ⅰ Thirty-six mice were divided into 2 groups (n=18 each) using a random number table method: control group (group C) and PND group.Group C only received isoflurane anesthesia.Partial left lobectomy of the liver was performed under isoflurane anesthesia in group PND.Contextual fear conditioning test was performed at 1, 3 and 7 days after surgery, and the freezing time was recorded.The mice were then sacrificed, and the hippocampus was isolated for determination of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) contents (by enzyme-linked immunosorbent assay) and CD200 and CD200R1 expression (by Western blot). Experiment Ⅱ Twenty-four mice were divided into 2 groups (n=12 each) using a random number table method: CD200-Fc group and IgG1-Fc group.Recombinant proteins CD200-Fc and human IgG1-Fc were injected into the lateral cerebral ventricle in CD200-Fc group and IgG1-Fc group, respectively.Partial left lobectomy of the liver was performed after the end of injection.Contextual fear conditioning test was performed at 1 and 3 days after surgery, and the freezing time was recorded. Results Experiment Ⅰ Compared with group C, the freezing time in the contextual fear conditioning test was significantly shortened, and the contents of IL-1β were increased at 1 and 3 days after surgery, the contents of TNF-α were increased at 3 and 7 days after surgery, and the expression of CD200 and CD200R1 was up-regulated at 1 day after surgery in group PND (P<0.05). Experiment Ⅱ Compared with IgG1-Fc group, the freezing time in the contextual fear conditioning test was significantly prolonged at 1 day after surgery in CD200-Fc group (P<0.05). Conclusion Up-regulated expression of hippocampal CD200R1 is the endogenous protective mechanism of PND in mice. Key words: Neurocognitive disorders; CD200R1
目的探讨海马CD200受体1 (CD200R1)在小鼠围手术期神经认知障碍(PND)中的作用。方法选用洁净级雄性C57BL/6小鼠60只，年龄9 ~ 10月龄，体重32 ~ 38 g。实验分两部分进行。实验Ⅰ36只小鼠采用随机数字表法分为2组，每组18只:对照组(C组)和PND组。C组仅异氟醚麻醉。PND组在异氟醚麻醉下行左肝部分肺叶切除术。分别于术后1、3、7天进行情境恐惧条件反射测试，记录冷冻时间。然后处死小鼠，分离海马，测定白细胞介素-1β (IL-1β)和肿瘤坏死因子-α (TNF-α)含量(酶联免疫吸附法)和CD200、CD200R1表达(Western blot法)。实验Ⅱ采用随机数字表法将24只小鼠分为2组，每组12只:CD200-Fc组和IgG1-Fc组。CD200-Fc组和IgG1-Fc组小鼠侧脑室分别注射重组蛋白CD200-Fc和人IgG1-Fc。注射结束后行左侧部分肝切除。分别于术后第1天和第3天进行情境恐惧条件反射测试，记录冷冻时间。结果实验Ⅰ与C组比较，PND组情境恐惧条件反射试验冻结时间显著缩短，术后1、3 d IL-1β含量升高，术后3、7 d TNF-α含量升高，术后1 d CD200、CD200R1表达上调(P<0.05)。实验Ⅱ与IgG1-Fc组比较，CD200-Fc组术后第1天情境恐惧条件反射测试冻结时间显著延长(P<0.05)。结论海马CD200R1表达上调是小鼠PND的内源性保护机制。关键词:神经认知障碍;CD200R1
{"title":"Role of hippocampal CD200R1 in perioperative neurocognitive disorders in mice","authors":"D. Ma, Jinhui Liu, Wenzhen Shen, Changwei Wei, Chao Xiong, Dandan Lin, Zi-Yi Xue, Anshi Wu","doi":"10.3760/CMA.J.ISSN.0254-1416.2019.10.009","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1416.2019.10.009","url":null,"abstract":"Objective \u0000To evaluate the role of hippocampal CD200 receptor 1 (CD200R1) in perioperative neurocognitive disorders (PND) in mice. \u0000 \u0000 \u0000Methods \u0000Sixty clean-grade male C57BL/6 mice, aged 9-10 months, weighing 32-38 g, were used in the study.The experiment was performed in two parts.Experiment Ⅰ Thirty-six mice were divided into 2 groups (n=18 each) using a random number table method: control group (group C) and PND group.Group C only received isoflurane anesthesia.Partial left lobectomy of the liver was performed under isoflurane anesthesia in group PND.Contextual fear conditioning test was performed at 1, 3 and 7 days after surgery, and the freezing time was recorded.The mice were then sacrificed, and the hippocampus was isolated for determination of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) contents (by enzyme-linked immunosorbent assay) and CD200 and CD200R1 expression (by Western blot). Experiment Ⅱ Twenty-four mice were divided into 2 groups (n=12 each) using a random number table method: CD200-Fc group and IgG1-Fc group.Recombinant proteins CD200-Fc and human IgG1-Fc were injected into the lateral cerebral ventricle in CD200-Fc group and IgG1-Fc group, respectively.Partial left lobectomy of the liver was performed after the end of injection.Contextual fear conditioning test was performed at 1 and 3 days after surgery, and the freezing time was recorded. \u0000 \u0000 \u0000Results \u0000Experiment Ⅰ Compared with group C, the freezing time in the contextual fear conditioning test was significantly shortened, and the contents of IL-1β were increased at 1 and 3 days after surgery, the contents of TNF-α were increased at 3 and 7 days after surgery, and the expression of CD200 and CD200R1 was up-regulated at 1 day after surgery in group PND (P<0.05). Experiment Ⅱ Compared with IgG1-Fc group, the freezing time in the contextual fear conditioning test was significantly prolonged at 1 day after surgery in CD200-Fc group (P<0.05). \u0000 \u0000 \u0000Conclusion \u0000Up-regulated expression of hippocampal CD200R1 is the endogenous protective mechanism of PND in mice. \u0000 \u0000 \u0000Key words: \u0000Neurocognitive disorders; CD200R1","PeriodicalId":10053,"journal":{"name":"中华麻醉学杂志","volume":"39 1","pages":"1181-1184"},"PeriodicalIF":0.0,"publicationDate":"2019-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41355579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-20DOI: 10.3760/CMA.J.ISSN.0254-1416.2019.10.023
Cheng Yu, N. Xie, Cui-Min Gao
Objective To evaluate the effect of ticagrelor on plasma P-selectin (CD62P) concentration during acute kidney injury in septic rats. Methods Thirty SPF healthy male Sprague-Dawley rats, aged 6-8 weeks, weighing 180-220 g, were divided into 3 groups(n=10 each)using a random number table method: sham operation group (group S), sepsis group (group Sep) and ticagrelor group (group T). Sepsis was induced by cecal ligation and puncture in anesthetized rats.The abdomen was opened after anesthesia induction and then closed after turning the intestines in group S. Ticagrelor 8.6 mg/kg was given through a gastric tube into stomach at 12 h after establishing the model in group T, while the equal volume of distilled water was given instead in S and Sep groups.Blood samples were collected from hearts at 24 h after establishing the model for determination of the concentrations of Scr and plasma CD62P.The rats were then sacrificed, and bilateral renal tissues were taken for examination of pathological changes and for determination of cell apoptosis by fluorescence microscopy after Annexin/V-FITC double staining was performed. Results Compared with group S, the concentrations of Scr and plasma CD62P, renal Paller score and apoptosis rate of renal cells were significantly increased in Sep and T groups (P<0.05). Compared with group Sep, the concentrations of Scr and plasma CD62P, renal Paller score and apoptosis rate of renal cells were significantly decreased in group T (P<0.05). Conclusion The mechanism by which ticagrelor reduces acute kidney injury is related to decreasing plasma CD62P concentrations and inhibiting cell apoptosis in septic rats. Key words: Platelet aggregation inhibitors; Sepsis; Acute kidney injury; P-selectin
{"title":"Effect of ticagrelor on plasma P-selectin concentration during acute kidney injury in septic rats","authors":"Cheng Yu, N. Xie, Cui-Min Gao","doi":"10.3760/CMA.J.ISSN.0254-1416.2019.10.023","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1416.2019.10.023","url":null,"abstract":"Objective \u0000To evaluate the effect of ticagrelor on plasma P-selectin (CD62P) concentration during acute kidney injury in septic rats. \u0000 \u0000 \u0000Methods \u0000Thirty SPF healthy male Sprague-Dawley rats, aged 6-8 weeks, weighing 180-220 g, were divided into 3 groups(n=10 each)using a random number table method: sham operation group (group S), sepsis group (group Sep) and ticagrelor group (group T). Sepsis was induced by cecal ligation and puncture in anesthetized rats.The abdomen was opened after anesthesia induction and then closed after turning the intestines in group S. Ticagrelor 8.6 mg/kg was given through a gastric tube into stomach at 12 h after establishing the model in group T, while the equal volume of distilled water was given instead in S and Sep groups.Blood samples were collected from hearts at 24 h after establishing the model for determination of the concentrations of Scr and plasma CD62P.The rats were then sacrificed, and bilateral renal tissues were taken for examination of pathological changes and for determination of cell apoptosis by fluorescence microscopy after Annexin/V-FITC double staining was performed. \u0000 \u0000 \u0000Results \u0000Compared with group S, the concentrations of Scr and plasma CD62P, renal Paller score and apoptosis rate of renal cells were significantly increased in Sep and T groups (P<0.05). Compared with group Sep, the concentrations of Scr and plasma CD62P, renal Paller score and apoptosis rate of renal cells were significantly decreased in group T (P<0.05). \u0000 \u0000 \u0000Conclusion \u0000The mechanism by which ticagrelor reduces acute kidney injury is related to decreasing plasma CD62P concentrations and inhibiting cell apoptosis in septic rats. \u0000 \u0000 \u0000Key words: \u0000Platelet aggregation inhibitors; Sepsis; Acute kidney injury; P-selectin","PeriodicalId":10053,"journal":{"name":"中华麻醉学杂志","volume":"39 1","pages":"1240-1242"},"PeriodicalIF":0.0,"publicationDate":"2019-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49556785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-20DOI: 10.3760/CMA.J.ISSN.0254-1416.2019.10.011
Guiping Xu, Juan-juan Fu, Xuan Zhao, Xiaoli Wang
Objective To evaluate the role of phosphatidylinositol-3-kinase/protein kinase B/nuclear factor erythroid 2-related factor 2 (PI3K/Akt/Nrf2) signaling pathway in resveratrol preconditioning-induced cardioprotection in diabetic rats. Methods Clean-grade healthy male Sprague-Dawley rats, aged 2-3 months, weighing 240-280 g, were used in the study.The diabetes model was established by intraperitoneal injection of streptozotocin 30 mg/kg once a day for 7 consecutive days.Forty diabetic rats were selected and divided into 4 groups (n=10 each) according to the random number table method: sham operation group (S group), myocardial ischemia-reperfusion (I/R) group (I/R group), resveratrol plus myocardial I/R group (Res+ I/R group), and PI3K inhibitor LY294002 plus resveratrol plus myocardial I/R group (LY+ Res+ I/R group). The myocardial I/R injury model was established by ligating the left anterior descending coronary artery for 30 min followed by 120-min reperfusion in anesthetized rats.Resveratrol 20 mg/kg was intraperitoneally injected once a day for 7 consecutive days at one week before surgery in Res+ I/R and LY+ Res+ I/R groups.LY294002 1.5 mg/kg was intraperitoneally injected at 30 min before operation in LY+ Res+ I/R group.After 120 min of reperfusion, blood samples were taken for determination of serum creatine kinase-MB (CK-MB) and lactic dehydrogenase (LDH) concentrations (by enzyme-linked immunosorbent assay), and myocardial tissues at the ischemic area were obtained for determination of superoxide dismutase (SOD), glutathione (GSH) and malondialdehyde (MDA)levels (by enzyme-linked immunosorbent assay) and expression of Akt, phosphorylated Akt (p-Akt), glycogen synthase kinase 3β (GSK3β), phosphorylated GSK3β (p-GSK3β) and Nrf2 (by Western blot). Results Compared with group S, serum CK-MB and LDH concentrations were significantly increased, the SOD and GSH levels were decreased, the MDA level was increased, and the expression of p-Akt and p-GSK3β was down-regulated in I/R group (P<0.05). Compared with I/R group, serum CK-MB and LDH concentrations were significantly decreased, the SOD and GSH levels were increased, the MDA level was decreased, and the expression of p-Akt, p-GSK3β and Nrf2 was up-regulated in Res+ I/R group (P<0.05). Compared with Res+ I/R group, serum CK-MB and LDH concentrations were significantly increased, the SOD and GSH levels were decreased, the MDA level was increased, and the expression of p-Akt, p-GSK3β and Nrf2 was down-regulated in LY+ Res+ I/R group (P<0.05). Conclusion The mechanism of resveratrol preconditioning-induced cardioprotection is related to activating PI3K/Akt/Nrf2 signaling pathway and inhibiting oxidative stress responses in diabetic rats. Key words: Myocardial reperfusion injury; Diabetes mellitus; Phenols; 1-phosphatidylinositol 3-kinase; Protein-serine-threonine kinases; NF-E2-related factor 2
{"title":"Role of PI3K/Akt/Nrf2 signaling pathway in resveratrol preconditioning-induced cardioprotection in diabetic rats","authors":"Guiping Xu, Juan-juan Fu, Xuan Zhao, Xiaoli Wang","doi":"10.3760/CMA.J.ISSN.0254-1416.2019.10.011","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1416.2019.10.011","url":null,"abstract":"Objective \u0000To evaluate the role of phosphatidylinositol-3-kinase/protein kinase B/nuclear factor erythroid 2-related factor 2 (PI3K/Akt/Nrf2) signaling pathway in resveratrol preconditioning-induced cardioprotection in diabetic rats. \u0000 \u0000 \u0000Methods \u0000Clean-grade healthy male Sprague-Dawley rats, aged 2-3 months, weighing 240-280 g, were used in the study.The diabetes model was established by intraperitoneal injection of streptozotocin 30 mg/kg once a day for 7 consecutive days.Forty diabetic rats were selected and divided into 4 groups (n=10 each) according to the random number table method: sham operation group (S group), myocardial ischemia-reperfusion (I/R) group (I/R group), resveratrol plus myocardial I/R group (Res+ I/R group), and PI3K inhibitor LY294002 plus resveratrol plus myocardial I/R group (LY+ Res+ I/R group). The myocardial I/R injury model was established by ligating the left anterior descending coronary artery for 30 min followed by 120-min reperfusion in anesthetized rats.Resveratrol 20 mg/kg was intraperitoneally injected once a day for 7 consecutive days at one week before surgery in Res+ I/R and LY+ Res+ I/R groups.LY294002 1.5 mg/kg was intraperitoneally injected at 30 min before operation in LY+ Res+ I/R group.After 120 min of reperfusion, blood samples were taken for determination of serum creatine kinase-MB (CK-MB) and lactic dehydrogenase (LDH) concentrations (by enzyme-linked immunosorbent assay), and myocardial tissues at the ischemic area were obtained for determination of superoxide dismutase (SOD), glutathione (GSH) and malondialdehyde (MDA)levels (by enzyme-linked immunosorbent assay) and expression of Akt, phosphorylated Akt (p-Akt), glycogen synthase kinase 3β (GSK3β), phosphorylated GSK3β (p-GSK3β) and Nrf2 (by Western blot). \u0000 \u0000 \u0000Results \u0000Compared with group S, serum CK-MB and LDH concentrations were significantly increased, the SOD and GSH levels were decreased, the MDA level was increased, and the expression of p-Akt and p-GSK3β was down-regulated in I/R group (P<0.05). Compared with I/R group, serum CK-MB and LDH concentrations were significantly decreased, the SOD and GSH levels were increased, the MDA level was decreased, and the expression of p-Akt, p-GSK3β and Nrf2 was up-regulated in Res+ I/R group (P<0.05). Compared with Res+ I/R group, serum CK-MB and LDH concentrations were significantly increased, the SOD and GSH levels were decreased, the MDA level was increased, and the expression of p-Akt, p-GSK3β and Nrf2 was down-regulated in LY+ Res+ I/R group (P<0.05). \u0000 \u0000 \u0000Conclusion \u0000The mechanism of resveratrol preconditioning-induced cardioprotection is related to activating PI3K/Akt/Nrf2 signaling pathway and inhibiting oxidative stress responses in diabetic rats. \u0000 \u0000 \u0000Key words: \u0000Myocardial reperfusion injury; Diabetes mellitus; Phenols; 1-phosphatidylinositol 3-kinase; Protein-serine-threonine kinases; NF-E2-related factor 2","PeriodicalId":10053,"journal":{"name":"中华麻醉学杂志","volume":"39 1","pages":"1189-1193"},"PeriodicalIF":0.0,"publicationDate":"2019-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43946681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-20DOI: 10.3760/CMA.J.ISSN.0254-1416.2019.10.012
Shouyuan Tian, Wenjie Zhang, Lixia Nie, Lili Wang, Zhijia Guo, Xiang Yu, Zhifang Fan
Objective To evaluate the effects of ketamine anesthesia on proteome in hippocampus in aged rats. Methods Thirty healthy male Wistar rats, aged 20 months, weighing 560-610 g, were divided into 2 groups (n=15 each) using a random number table method: control group (group C) and ketamine group (group K). In group K, ketamine 80 mg/kg was intraperitoneally injected, additional 1/2 initial dose was given when the righting reflex was recovered, and anesthesia was maintained for 3 h. Morris water maze test was performed starting from 1st day after the end of anesthesia.Five rats were selected at days 1 and 7 after the end of anesthesia and sacrificed, and hippocampal tissues were obtained to extract proteins.Proteins extracted from rat hippocampi were identified by 2-dimensional electrophoresis (2-DE). The differentially expressed proteins were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and biological information system. Results Compared with group C, the escape latency and total swimming distance to find the submerged platform in Morris water maze at the 1st day after anesthesia were significantly prolonged in group K (P<0.05 or 0.01). The MALDI-TOF-MS analysis showed that there were 21 differentially expressed proteins at 1st day after ketamine anesthesia, of which 6 proteins (involving maintenance of intracellular protein homeostasis, energy metabolism, etc.) presented with up-regulated expression and 15 proteins (involving synaptic vesicle transport efficiency, synaptic structural and functional plasticity, maintenance of intracellular protein homeostasis, NMDA-mediated Ca2+ signal transport, energy metabolism, etc.) presented with down-regulated expression.There were 8 differentially expressed proteins at 7th day, including 3 proteins with up-regulated expression and 5 proteins with down-regulated expression (P<0.05). Conclusion Ketamine anesthesia can induce 21 differentially expressed proteins in hippocampi of aged rats, involving synaptic vesicle transport efficiency, synaptic structural and functional plasticity, intracellular protein homeostasis, NMDA-mediated Ca2+ signal transport, energy metabolism, and etc.which may be involved in the mechanism of ketamine-induced temporary cognitive dysfunction. Key words: Ketamine; Cognitive function; Hippocampus; Proteome; Aged
{"title":"Effect of ketamine anesthesia on proteome in hippocampus of aged rats","authors":"Shouyuan Tian, Wenjie Zhang, Lixia Nie, Lili Wang, Zhijia Guo, Xiang Yu, Zhifang Fan","doi":"10.3760/CMA.J.ISSN.0254-1416.2019.10.012","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1416.2019.10.012","url":null,"abstract":"Objective \u0000To evaluate the effects of ketamine anesthesia on proteome in hippocampus in aged rats. \u0000 \u0000 \u0000Methods \u0000Thirty healthy male Wistar rats, aged 20 months, weighing 560-610 g, were divided into 2 groups (n=15 each) using a random number table method: control group (group C) and ketamine group (group K). In group K, ketamine 80 mg/kg was intraperitoneally injected, additional 1/2 initial dose was given when the righting reflex was recovered, and anesthesia was maintained for 3 h. Morris water maze test was performed starting from 1st day after the end of anesthesia.Five rats were selected at days 1 and 7 after the end of anesthesia and sacrificed, and hippocampal tissues were obtained to extract proteins.Proteins extracted from rat hippocampi were identified by 2-dimensional electrophoresis (2-DE). The differentially expressed proteins were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and biological information system. \u0000 \u0000 \u0000Results \u0000Compared with group C, the escape latency and total swimming distance to find the submerged platform in Morris water maze at the 1st day after anesthesia were significantly prolonged in group K (P<0.05 or 0.01). The MALDI-TOF-MS analysis showed that there were 21 differentially expressed proteins at 1st day after ketamine anesthesia, of which 6 proteins (involving maintenance of intracellular protein homeostasis, energy metabolism, etc.) presented with up-regulated expression and 15 proteins (involving synaptic vesicle transport efficiency, synaptic structural and functional plasticity, maintenance of intracellular protein homeostasis, NMDA-mediated Ca2+ signal transport, energy metabolism, etc.) presented with down-regulated expression.There were 8 differentially expressed proteins at 7th day, including 3 proteins with up-regulated expression and 5 proteins with down-regulated expression (P<0.05). \u0000 \u0000 \u0000Conclusion \u0000Ketamine anesthesia can induce 21 differentially expressed proteins in hippocampi of aged rats, involving synaptic vesicle transport efficiency, synaptic structural and functional plasticity, intracellular protein homeostasis, NMDA-mediated Ca2+ signal transport, energy metabolism, and etc.which may be involved in the mechanism of ketamine-induced temporary cognitive dysfunction. \u0000 \u0000 \u0000Key words: \u0000Ketamine; Cognitive function; Hippocampus; Proteome; Aged","PeriodicalId":10053,"journal":{"name":"中华麻醉学杂志","volume":"39 1","pages":"1194-1198"},"PeriodicalIF":0.0,"publicationDate":"2019-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44437834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-20DOI: 10.3760/CMA.J.ISSN.0254-1416.2019.10.014
Jingjing Jiang, Heng Li, Baojun Fu, Weihua Liu, Zonghang Lin
Objective To evaluate the role of 2B-containing NMDA receptors (NR2B) in sevoflurane anesthesia-induced cognitive dysfunction in aged rats. Methods Thirty-two healthy male Sprague-Dawley rats, aged 18 months, weighing 570-630 g, were divided into 4 groups (n=8 each) using a random number table method: control group (group C), sevoflurane anesthesia group (group S), sevoflurane anesthesia plus NR2B specific inhibitor Ro 25-6981 group (group S+ RO) and Ro 25-6981 group (group RO). S and S+ RO groups inhaled 3% sevoflurane for 4 h. Ro 25-6981 1 mg/kg was intraperitoneally injected at 15 min before inhaling sevoflurane in group S+ RO.Morris water maze test was performed at 2 days after the end of anesthesia to assess cognitive function.The rats were then sacrificed, and hippocampal tissues were obtained for determination of the expression and phosphorylation of ERK1/2 by Western blot. Results Compared with group C, the escape latency was significantly prolonged, the frequency of crossing the original platform was reduced, the time of staying at the original platform quadrant was shortened, and the phosphorylation of ERK1/2 was decreased in group S (P 0.05). Compared with group S, the escape latency was significantly shortened, the frequency of crossing the original platform was increased, the time of staying at the original platform quadrant was prolonged, and the phosphorylation of ERK1/2 was increased in group S+ RO(P 0.05). Conclusion The mechanism by which sevoflurane anesthesia induces cognitive dysfunction is related to up-regulating the expression of NR2B and inhibiting the activity of ERK1/2 in aged rats. Key words: Receptors, N-methyl-D-aspartate; Anesthetics, inhalation; Cognition disorders; Aged
{"title":"Role of NR2B in sevoflurane anesthesia-induced cognitive dysfunction in aged rats","authors":"Jingjing Jiang, Heng Li, Baojun Fu, Weihua Liu, Zonghang Lin","doi":"10.3760/CMA.J.ISSN.0254-1416.2019.10.014","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1416.2019.10.014","url":null,"abstract":"Objective \u0000To evaluate the role of 2B-containing NMDA receptors (NR2B) in sevoflurane anesthesia-induced cognitive dysfunction in aged rats. \u0000 \u0000 \u0000Methods \u0000Thirty-two healthy male Sprague-Dawley rats, aged 18 months, weighing 570-630 g, were divided into 4 groups (n=8 each) using a random number table method: control group (group C), sevoflurane anesthesia group (group S), sevoflurane anesthesia plus NR2B specific inhibitor Ro 25-6981 group (group S+ RO) and Ro 25-6981 group (group RO). S and S+ RO groups inhaled 3% sevoflurane for 4 h. Ro 25-6981 1 mg/kg was intraperitoneally injected at 15 min before inhaling sevoflurane in group S+ RO.Morris water maze test was performed at 2 days after the end of anesthesia to assess cognitive function.The rats were then sacrificed, and hippocampal tissues were obtained for determination of the expression and phosphorylation of ERK1/2 by Western blot. \u0000 \u0000 \u0000Results \u0000Compared with group C, the escape latency was significantly prolonged, the frequency of crossing the original platform was reduced, the time of staying at the original platform quadrant was shortened, and the phosphorylation of ERK1/2 was decreased in group S (P 0.05). Compared with group S, the escape latency was significantly shortened, the frequency of crossing the original platform was increased, the time of staying at the original platform quadrant was prolonged, and the phosphorylation of ERK1/2 was increased in group S+ RO(P 0.05). \u0000 \u0000 \u0000Conclusion \u0000The mechanism by which sevoflurane anesthesia induces cognitive dysfunction is related to up-regulating the expression of NR2B and inhibiting the activity of ERK1/2 in aged rats. \u0000 \u0000 \u0000Key words: \u0000Receptors, N-methyl-D-aspartate; Anesthetics, inhalation; Cognition disorders; Aged","PeriodicalId":10053,"journal":{"name":"中华麻醉学杂志","volume":"39 1","pages":"1203-1206"},"PeriodicalIF":0.0,"publicationDate":"2019-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42701771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-20DOI: 10.3760/CMA.J.ISSN.0254-1416.2019.10.027
Shuang Han, Kun He, Junfang Rong
Objective To evaluate the effect of ixeris sonchifolia pretreatment on the expression of hypoxia-inducible factor-1α (HIF-1α) following myocardial injury induced by exhausting exercise in rats. Methods Fifty-six healthy clean-grade male Wistar rats, weighing 200-220 g, were divided into 3 groups using a random number table method: control group (group C, n=8), exhausting exercise group (group E, n=24) and ixeris sonchifolia pretreatment group (group IS, n=24). In E and IS groups, the model of myocardial injury was established by exhausting swimming.In group IS, the rats were subjected to exhausting swimming after intraperitoneal ixeris sonehifolia 20 ml/kg.In E and IS groups, blood samples were taken from the inferior vena cava at 0, 6 and 24 h after exhaustion (T1-3) for determination of serum cardiac troponin I (cTnI) concentrations by enzyme-linked immunosorbent assay.The animals were sacrificed after anesthesia, and myocardial specimens were obtained for determination of the cell apoptosis index (by TUNEL) and expression of HIF-1α, Bax and Bcl-2 (by immunohistochemistry), and Bax/Bcl-2 ratio was calculated.The area of myocardial injury was observed using HBFP assay. Results Compared with group C, the area of myocardial injury, concentration of serum cTnl, Bax/Bcl-2 ratio and apoptosis index were significantly increased, and the expression of HIF-1α in myocardial tissues was up-regulated at each time point in E and IS groups (P<0.05). Compared with group E, the area of myocardial injury, concentration of serum cTnl, Bax/Bcl-2 ratio and apoptosis index were significantly decreased, and the expression of HIF-1α in myocardial tissues was down-regulated at each time point in group IS(P<0.05). Conclusion The mechanism by which ixeris sonchifolia pretreatment mitigates myocardial injury induced by exhausting exercise is related to inhibiting up-regulated expression of HIF-1α in myocardial tissues and reducing cell apoptosis in rats. Key words: Asteraceae; Hyperkinesis; Cardiomyopathies; Hypoxia-inducible factor 1, alpha subunit
{"title":"Effect of ixeris sonchifolia pretreatment on expression of hypoxia-inducible factor-1α following myocardial injury induced by exhausting exercise in rats","authors":"Shuang Han, Kun He, Junfang Rong","doi":"10.3760/CMA.J.ISSN.0254-1416.2019.10.027","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1416.2019.10.027","url":null,"abstract":"Objective \u0000To evaluate the effect of ixeris sonchifolia pretreatment on the expression of hypoxia-inducible factor-1α (HIF-1α) following myocardial injury induced by exhausting exercise in rats. \u0000 \u0000 \u0000Methods \u0000Fifty-six healthy clean-grade male Wistar rats, weighing 200-220 g, were divided into 3 groups using a random number table method: control group (group C, n=8), exhausting exercise group (group E, n=24) and ixeris sonchifolia pretreatment group (group IS, n=24). In E and IS groups, the model of myocardial injury was established by exhausting swimming.In group IS, the rats were subjected to exhausting swimming after intraperitoneal ixeris sonehifolia 20 ml/kg.In E and IS groups, blood samples were taken from the inferior vena cava at 0, 6 and 24 h after exhaustion (T1-3) for determination of serum cardiac troponin I (cTnI) concentrations by enzyme-linked immunosorbent assay.The animals were sacrificed after anesthesia, and myocardial specimens were obtained for determination of the cell apoptosis index (by TUNEL) and expression of HIF-1α, Bax and Bcl-2 (by immunohistochemistry), and Bax/Bcl-2 ratio was calculated.The area of myocardial injury was observed using HBFP assay. \u0000 \u0000 \u0000Results \u0000Compared with group C, the area of myocardial injury, concentration of serum cTnl, Bax/Bcl-2 ratio and apoptosis index were significantly increased, and the expression of HIF-1α in myocardial tissues was up-regulated at each time point in E and IS groups (P<0.05). Compared with group E, the area of myocardial injury, concentration of serum cTnl, Bax/Bcl-2 ratio and apoptosis index were significantly decreased, and the expression of HIF-1α in myocardial tissues was down-regulated at each time point in group IS(P<0.05). \u0000 \u0000 \u0000Conclusion \u0000The mechanism by which ixeris sonchifolia pretreatment mitigates myocardial injury induced by exhausting exercise is related to inhibiting up-regulated expression of HIF-1α in myocardial tissues and reducing cell apoptosis in rats. \u0000 \u0000 \u0000Key words: \u0000Asteraceae; Hyperkinesis; Cardiomyopathies; Hypoxia-inducible factor 1, alpha subunit","PeriodicalId":10053,"journal":{"name":"中华麻醉学杂志","volume":"39 1","pages":"1258-1260"},"PeriodicalIF":0.0,"publicationDate":"2019-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46965866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-20DOI: 10.3760/CMA.J.ISSN.0254-1416.2019.10.028
L. Ding
{"title":"Effects of dexmedetomidine on perioperative systemic inflammatory responses of elderly patients undergoing radical operation for intestinal cancer","authors":"L. Ding","doi":"10.3760/CMA.J.ISSN.0254-1416.2019.10.028","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1416.2019.10.028","url":null,"abstract":"","PeriodicalId":10053,"journal":{"name":"中华麻醉学杂志","volume":"39 1","pages":"1261-1262"},"PeriodicalIF":0.0,"publicationDate":"2019-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46894345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-20DOI: 10.3760/CMA.J.ISSN.0254-1416.2019.10.008
Murat Marjan, Zhi-Hua Wang, Cheng Chen, Y. Huang, Ping-ping Xia, Fan Zhang, E. Wang, Q. Guo, Z. Ye
Objective To evaluate the role of long non-coding RNA maternally expressed gene 3 (MEG3) in hyperglycose-induced neurocyte damage and the relationship with mitochondrion-dependent apoptosis in rats. Methods Normally cultured PC12 cells were divided into 5 groups (n=18 each) using a random number table method: normal concentration of glucose control group (C group), normal concentration of glucose plus MEG3 group (C+ MEG3 group), high-concentration glucose group (HG group), high-concentration glucose plus MEG3 group(HG+ MEG3 group), and high-concentration glucose plus negative lentiviral vector (LV-NC) group (HG+ NC group). PC12 cells were cultured in DMEM medium with 25 mmol/L glucose in group C. PC12 cells were cultured in DMEM medium with 25 mmol/L glucose after being transfected with MEG3 lentiviral vector (LV-MEG3) in C+ MEG3 group.PC12 cells were cultured in DMEM medium with 250 mmol/L glucose in HG group.PC12 cells were incubated in DMEM medium containing 250 mmol/L glucose after being transfected with LV-MEG3 in HG+ MEG3 group.PC12 cells were incubated in DMEM medium containing 250 mmol/L glucose after being transfected with LV-NC in HG+ NC group.After the cells were cultured or incubated for 1 day, the cell viability was measured by CCK8 assay, the apoptosis rate and level of reactive oxygen species (ROS) were determined by flow cytometry, and the amount of lactic dehydrogenase (LDH) released was measured by DCFH-DA, the expression of Cyt c, caspase-3, caspase-9, Bcl-2, Bax and Apaf-1 was determined by Western blot, and the opening of mitochondrial permeability transition pore (mPTP) was determined by fluorescent method.Blc-2/Bax ratio was calculated. Results Compared with group C, the cell viability was significantly decreased, the amount of LDH released, ROS level and apoptosis rate were increased, the opening of mPTP was increased, and the expression of caspase-3, caspase-9, Cyt c, Bax, Bcl-2 and Apaf-1 was up-regulated in HG, HG+ MEG3 and HG+ NC groups, and Bcl-2/Bax ratio was increased in HG+ MEG3 group and decreased in HG and HG+ NC groups (P<0.05). Compared with HG group and HG+ NC group, the cell activity was significantly increased, the amount of LDH released, ROS level and apoptosis rate were decreased, the opening of mPTP was decreased, the expression of caspase-3, caspase-9, Cyt c, Bax, and Apaf-1 was down-regulated, the expression of Bcl-2 was up-regulated, and Bcl-2/Bax ratio was increased in HG+ MEG3 group (P<0.01). Conclusion MEG3 may be involved in the endogenous protective mechanism during hyperglycose-induced neurocyte damage by inhibiting mitochondrion-dependent apoptosis in rats. Key words: RNA, long noncoding; Diabetes mellitus; Neurons; Apoptosis
{"title":"Role of long non-coding RNA MEG3 in hyperglycose-induced neurocyte damage in rats: relationship with mitochondrion-dependent apoptosis","authors":"Murat Marjan, Zhi-Hua Wang, Cheng Chen, Y. Huang, Ping-ping Xia, Fan Zhang, E. Wang, Q. Guo, Z. Ye","doi":"10.3760/CMA.J.ISSN.0254-1416.2019.10.008","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1416.2019.10.008","url":null,"abstract":"Objective \u0000To evaluate the role of long non-coding RNA maternally expressed gene 3 (MEG3) in hyperglycose-induced neurocyte damage and the relationship with mitochondrion-dependent apoptosis in rats. \u0000 \u0000 \u0000Methods \u0000Normally cultured PC12 cells were divided into 5 groups (n=18 each) using a random number table method: normal concentration of glucose control group (C group), normal concentration of glucose plus MEG3 group (C+ MEG3 group), high-concentration glucose group (HG group), high-concentration glucose plus MEG3 group(HG+ MEG3 group), and high-concentration glucose plus negative lentiviral vector (LV-NC) group (HG+ NC group). PC12 cells were cultured in DMEM medium with 25 mmol/L glucose in group C. PC12 cells were cultured in DMEM medium with 25 mmol/L glucose after being transfected with MEG3 lentiviral vector (LV-MEG3) in C+ MEG3 group.PC12 cells were cultured in DMEM medium with 250 mmol/L glucose in HG group.PC12 cells were incubated in DMEM medium containing 250 mmol/L glucose after being transfected with LV-MEG3 in HG+ MEG3 group.PC12 cells were incubated in DMEM medium containing 250 mmol/L glucose after being transfected with LV-NC in HG+ NC group.After the cells were cultured or incubated for 1 day, the cell viability was measured by CCK8 assay, the apoptosis rate and level of reactive oxygen species (ROS) were determined by flow cytometry, and the amount of lactic dehydrogenase (LDH) released was measured by DCFH-DA, the expression of Cyt c, caspase-3, caspase-9, Bcl-2, Bax and Apaf-1 was determined by Western blot, and the opening of mitochondrial permeability transition pore (mPTP) was determined by fluorescent method.Blc-2/Bax ratio was calculated. \u0000 \u0000 \u0000Results \u0000Compared with group C, the cell viability was significantly decreased, the amount of LDH released, ROS level and apoptosis rate were increased, the opening of mPTP was increased, and the expression of caspase-3, caspase-9, Cyt c, Bax, Bcl-2 and Apaf-1 was up-regulated in HG, HG+ MEG3 and HG+ NC groups, and Bcl-2/Bax ratio was increased in HG+ MEG3 group and decreased in HG and HG+ NC groups (P<0.05). Compared with HG group and HG+ NC group, the cell activity was significantly increased, the amount of LDH released, ROS level and apoptosis rate were decreased, the opening of mPTP was decreased, the expression of caspase-3, caspase-9, Cyt c, Bax, and Apaf-1 was down-regulated, the expression of Bcl-2 was up-regulated, and Bcl-2/Bax ratio was increased in HG+ MEG3 group (P<0.01). \u0000 \u0000 \u0000Conclusion \u0000MEG3 may be involved in the endogenous protective mechanism during hyperglycose-induced neurocyte damage by inhibiting mitochondrion-dependent apoptosis in rats. \u0000 \u0000 \u0000Key words: \u0000RNA, long noncoding; Diabetes mellitus; Neurons; Apoptosis","PeriodicalId":10053,"journal":{"name":"中华麻醉学杂志","volume":"39 1","pages":"1176-1180"},"PeriodicalIF":0.0,"publicationDate":"2019-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42737468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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