中华麻醉学杂志最新文献

{"title":"Role of Nrf2-Gpx4 signaling pathway in Shenmai injection-induced reduction of myocardial ischemia-reperfusion injury: relationship with ferroptosis in rats","authors":"Sheng-lan Mei, Z. Xia, Xiao-jing Wu, S. Lei, Qingtao Meng, Zhen Qiu, Bin Zhou, H. Ming, Jinjian Zhou","doi":"10.3760/CMA.J.ISSN.0254-1416.2019.11.031","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1416.2019.11.031","url":null,"abstract":"Objective \u0000To evaluate the role of nuclear factor erythroid 2-related factor 2 (Nrf2)-glutathione peroxidase 4 (GPX4) signaling pathway in Shenmai injection-induced reduction of myocardial ischemia-reperfusion (I/R) injury and the relationship with ferroptosis in rats. \u0000 \u0000 \u0000Methods \u0000Forty-eight SPF healthy adult male Sprague-Dawley rats, weighing 220-250 g, were divided into 4 groups (n=12 each) by a random number table method: sham operation group (S group), myocardial I/R group (IR group), Shenmai injection group (SM group), and Shenmai injection plus Nrf2 inhibitor group (SMM group). Acute myocardial I/R injury was induced by ligating the anterior descending brach of the left coronary artery for 30 min followed by 120-min reperfusion in anesthetized rats.In SM group, Shenmai injection 9 ml/kg was intravenously injected immediately before reperfusion.In group SMM, Nrf2 inhibitor ML385 30 mg/kg was intraperitoneally injected at 30 min before ischemia, and Shenmai injection 9 ml/kg was intravenously injected immediately before reperfusion.Six rats in each group were selected at 120 min of reperfusion, blood samples were collected from the left ventricle, the rats were then sacrificed, specimens were obtained from cardiac apex for examination of the ultrastructure and for determination of serum cardiac tropomin I( cTnI) level (by enzyme-linked immunosorbent assay), contents of Fe2+ and malondialdehyde (MDA) and superoxide dismutase (SOD) activity (by colometry), and expression of Nrf2, GPX4 and AcylCoA synthetase longchain family member 4 (ACSL4) (by Western blot). Another 6 rats in each group were selected to measure the myocardial infarct size. \u0000 \u0000 \u0000Results \u0000Compared with group S, the myocardial infarct size, serum cTnI concentrations, and myocardial Fe2+ and MDA contents were significantly increased, the activity of SOD was decreased, the expression of Nrf2 and GPX4 was down-regulated, and the expression of ACSL4 was up-regulated in IR, SM and SMM groups (P 0.05). Compared with group SM, the myocardial infarct size, serum cTnI concentrations, and myocardial Fe2+ contents were significantly increased, the activity of SOD was decreased, the expression of Nrf2 and GPX4 was down-regulated, and the expression of ACSL4 was up-regulated in group SMM (P<0.05). \u0000 \u0000 \u0000Conclusion \u0000Activation of Nrf2-Gpx4 signaling pathway is involved in Shenmai injection-induced reduction of myocardial I/R injury and is related to inhibiting ferroptosis in rats. \u0000 \u0000 \u0000Key words: \u0000NF-E2-related factor 2; Glutathione peroxidase; SAPONINS; Myocardial reperfusion injury; Cell death","PeriodicalId":10053,"journal":{"name":"中华麻醉学杂志","volume":"39 1","pages":"1395-1398"},"PeriodicalIF":0.0,"publicationDate":"2019-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46527255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of butorphanol combined with saphenous never block on rehabilitation after total knee arthroplasty in elderly patients","authors":"Wen-qi Xin, Jun-jun Huang, Zeng Yan, Qing Yang, Aixiang Li, Yixuan Zhang","doi":"10.3760/CMA.J.ISSN.0254-1416.2019.11.035","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1416.2019.11.035","url":null,"abstract":"","PeriodicalId":10053,"journal":{"name":"中华麻醉学杂志","volume":"39 1","pages":"1407-1408"},"PeriodicalIF":0.0,"publicationDate":"2019-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47891740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of new technique in general anesthesia monitoring: what else can you see beyond EEG?","authors":"Hailong Dong, Wen Li","doi":"10.3760/CMA.J.ISSN.0254-1416.2019.11.002","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1416.2019.11.002","url":null,"abstract":"","PeriodicalId":10053,"journal":{"name":"中华麻醉学杂志","volume":"39 1","pages":"1284-1286"},"PeriodicalIF":0.0,"publicationDate":"2019-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46448578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Superior effect of dexmedetomidine used for induction of general anesthesia: prolonging duration of non-hypoxic apnoea","authors":"Yunbin Xie, Jin Wu, Yan Xia, Yongfeng Zheng, Xiaotian Liu, L. Min, Dong-hua Shao","doi":"10.3760/CMA.J.ISSN.0254-1416.2019.11.023","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1416.2019.11.023","url":null,"abstract":"Objective \u0000To evaluate the effect of dexmedetomidine on the duration of non-hypoxic apnoea when used for induction of general anesthesia. \u0000 \u0000 \u0000Methods \u0000Eighty female patients, aged 18-64 yr, with body mass index of 18.5-24.9 kg/m2, of American Society of Anesthesiology physical status Ⅰ or Ⅱ, with Mallampati grade Ⅰ or Ⅱ, scheduled for elective surgery with general anesthesia requiring tracheal intubation, were divided into 2 groups (n=40 each) by a random number table method: dexmedetomidine group (group D) and conventional group (group C). Dexmedetomidine 0.5 μg/kg was intravenously infused over 10 min in group D, and the normal saline 40 ml was intravenously infused in group C. In two groups, pure oxygen 8 L/min was inhaled via the closed mask, 3 min later fentanyl 3 μg/kg, etomidate 0.3 mg/kg and rocuronium 0.6 mg/kg were intravenously injected in turn, assisted ventilation was not performed in this period.The anesthesia machine was immediately connected to perform mechanical ventilation when SpO2 was reduced to 92% after tracheal intubation.Arterial blood samples were collected for blood gas analysis, and PaO2 was recorded at the time of entering the operation room, immediately before inhaling pure oxygen, at the beginning of injecting fentanyl, and when SpO2 was decreased to 92%.The development of adverse cardiovascular events during the trial, and duration of non-hypoxic apnoea were recorded. \u0000 \u0000 \u0000Results \u0000Compared with group C, the incidence of adverse cardiovascular events was significantly decreased, and the duration of non-hypoxic apnoea was prolonged, and no significant change was found in PaO2 at the time of entering the operation room, immediately before inhaling pure oxygen, at the beginning of injecting fentanyl, and when SpO2 was decreased to 92% in group D (P<0.05). \u0000 \u0000 \u0000Conclusion \u0000Dexmedetomidine can prolong the duration of non-hypoxic apnoea and raise the safety of endotracheal intubation during general anesthesia induction when used for induction of general anesthesia. \u0000 \u0000 \u0000Key words: \u0000Dexmedetomidine; Anesthesia, general; Duration of non-hypoxic apnoea","PeriodicalId":10053,"journal":{"name":"中华麻醉学杂志","volume":"39 1","pages":"1364-1366"},"PeriodicalIF":0.0,"publicationDate":"2019-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43664600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of effects of different labor analgesia regimens on perinatal mental state of parturients","authors":"Yu-qi Liu, Y. Nie, Jing Jiao, Shaoqiang Huang, Q. Luo","doi":"10.3760/CMA.J.ISSN.0254-1416.2019.11.012","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1416.2019.11.012","url":null,"abstract":"Objective \u0000To compare the effects of sufentanil mixed with ropivacaine and ropivacaine alone for peripartum analgesia on perinatal mental state of parturients. \u0000 \u0000 \u0000Methods \u0000Three hundred and eighty-nine primiparous acquiring labor analgesia in our hospital from April 2016 to April 2019, of American Society of Anesthesiologists physical status Ⅰ or Ⅱ, at ≥37 weeks of gestation, aged 25-45 yr, were divided into 2 groups using a random number table method: ropivacaine group (R group, n=193) and ropivacaine mixed with sufentanil group (RS group, n=196). Epidural labor analgesia was performed with 1.5% ropivacaine in group R. Epidural labor analgesia was performed with 1% ropivacaine mixed with 0.5 μg/ml sufentanil in group RS.The Edinburgh depression scale (EPDS), multidimensional social support perception scale (MSPSS) and breast-feeding self-brief scale (BSES) were used to evaluate the maternal psychological status at 30 min after labor analgesia induction and 42 days after delivery. \u0000 \u0000 \u0000Results \u0000One hundred and sixty-one patients completed the study in group RS and 157 patients in group R. Compared with group RS, the EPDS score was significantly decreased, and the BSES score was increased at 42 days after delivery (P 0.05). \u0000 \u0000 \u0000Conclusion \u0000Compared with epidural labor analgesia with ropivacaine, epidural labor analgesia with sufentanil mixed with ropivacaine has a marked adverse effect on the perinatal mental state of parturients. \u0000 \u0000 \u0000Key words: \u0000Analgesia, obstetrical; Depression, postpartum","PeriodicalId":10053,"journal":{"name":"中华麻醉学杂志","volume":"39 1","pages":"1322-1325"},"PeriodicalIF":0.0,"publicationDate":"2019-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49580851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of different doses of dexmedetomidine on lung injury during laparoscopic gallbladder surgery in elderly patients with mild obstructive ventilation dysfunction","authors":"Ji Ma, Haiyun Wang, Huaqing Wei, Junzhang Xiao, H. Mou, Mingshu Zhao, Qingkai Tang","doi":"10.3760/CMA.J.ISSN.0254-1416.2019.11.020","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1416.2019.11.020","url":null,"abstract":"Objective \u0000To evaluate the effect of different doses of dexmedetomidine on the lung injury during laparoscopic gallbladder surgery in elderly patients with mild obstructive ventilation dysfunction. \u0000 \u0000 \u0000Methods \u0000One hundred and twenty patients of both sexes, aged 65-75 yr, with body mass index of 18.5-23.9 kg/m2, of American Society of Anesthesiologists physical status Ⅱ or Ⅲ, who were diagnosed with mild obstructive ventilation dysfunction during the preoperative pulmonary function test, scheduled for elective laparoscopic gallbladder surgery under general anesthesia, were divided into 4 groups (n = 30 each) using a random number table method: control group (group C) and different doses of dexmedetomidine groups (Dex1, Dex2 and Dex3 groups). In Dex1, Dex2 and Dex3 groups, dexmedetomidine was intravenously infused as a bolus of 1 μg/kg over 10 min, followed by an infusion of 0.2, 0.4 and 0.6 μg·kg-1·h-1 until the end of operation, respectively.The equal volume of normal saline was given instead in group C. Airway peak pressure (Ppeak), mean airway pressure (Pmean), airway plateau pressure (Pplat), and positive end-expiratory pressure were measured immediately before pneumoperitoneum (T1), at the end of pneumoperitoneum (T2), and 10 min after the end of pneumoperitoneum (T3), and driving pressure was calculated.Arterial blood samples were obtained to record PaO2 and PaCO2, and oxygenation index (OI), respiratory index (RI), physiologic dead space fraction (VD/VT) and alveolar-arterial oxygen difference (A-aDO2) were calculated.The extubation time and development of complications such as hypercapnia and hypoxemia within 48 h after operation were recorded. \u0000 \u0000 \u0000Results \u0000Compared with group C, Ppeak, Pmean and driving pressure were significantly decreased, OI was increased, and the RI, VD/VT and A-aDO2 were decreased at T1-3, the intraoperative consumption of norepinephrine and atropine was increased, the extubation time was shortened, and the incidence of hyoxemia was decreased after operation in Dex1, Dex2 and Dex3 groups (P<0.05). Compared with group Dex1, Ppeak, Pmean and driving pressure were significantly decreased, OI was increased, and the RI, VD/VT and A-aDO2 were decreased at T1-3 in Dex2 and Dex3 groups, and the intraoperative consumption of norepinephrine and atropine was increased in group Dex3 (P<0.05). The intraoperative consumption of norepinephrine and atropine was significantly higher in group Dex3 than in group Dex2 (P<0.05). \u0000 \u0000 \u0000Conclusion \u0000The optimal maintenance dose of dexmedetomidine in improving pulmonary function during laparoscopic gallbladder surgery is 0.4 μg·kg-1·h-1 in elderly patients with mild obstructive ventilation dysfunction. \u0000 \u0000 \u0000Key words: \u0000Dexmedetomidine; Laparoscopic surgery; Respiratory function tests; Aged","PeriodicalId":10053,"journal":{"name":"中华麻醉学杂志","volume":"39 1","pages":"1352-1356"},"PeriodicalIF":0.0,"publicationDate":"2019-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46013476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of model for predicting mortality risk after abdominal surgery using preoperative indices based on different machine learning algorithms","authors":"Hong-Yu Zhi, Mengyue Gu, Yu-jie Li, Zhi-Yong Yang, Kunhua Zhong, Yuwen Chen, Ju Zhang, B. Yi, K. Lu","doi":"10.3760/CMA.J.ISSN.0254-1416.2019.11.003","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1416.2019.11.003","url":null,"abstract":"Objective \u0000To establish the model for predicting the mortality risk after abdominal surgery using preoperative indices based on different machine learning algorithms. \u0000 \u0000 \u0000Methods \u0000Fifty patients died after abdominal surgery with general anesthesia from June 2015 to December 2018 in our hospital were enrolled in the study.Based on the types of surgery and age of dead patients, 150 patients who were discharged from hospital upon recovery postoperatively were randomly selected from our database as control cases with a ratio of 1∶3.The total dataset of 200 patients was randomly divided into training dataset (n=140) and testing dataset (n=60). Preoperative indices (each index of baseline characteristics, each index of anesthesia interview information and indices of preoperative examination) were used to develop the model for predicting the mortality risk after abdominal surgery based on four machine learning algorithms AdaBoost, GBDT, LR, and SVM, and the model was evaluated in the testing dataset. \u0000 \u0000 \u0000Results \u0000The area under the receiver operating characteristic curves of models developed using preoperative index based on AdaBoost, GBDT, LR, and SVM for predicting the postoperative mortality risk were 0.796, 0.794, 0.846 and 0.781, respectively.There were no significant differences in area under the receiver operating characteristic curves among different models (P>0.05). \u0000 \u0000 \u0000Conclusion \u0000The model for predicting mortality risk after abdominal surgery using preoperative indicators based on different machine learning algorithms is successfully established. \u0000 \u0000 \u0000Key words: \u0000Artificial intelligence; Machine learning; Forecasting; Death; Postoperative complications","PeriodicalId":10053,"journal":{"name":"中华麻醉学杂志","volume":"39 1","pages":"1287-1290"},"PeriodicalIF":0.0,"publicationDate":"2019-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46386815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in expression of caspase recruitment domain protein 3 during ventilator-induced lung injury in rats","authors":"Benwang Zhang, Qiu-jie Li, Qiang Wang, Fu-guo Ma, Lixin Sun, Mingshan Wang","doi":"10.3760/CMA.J.ISSN.0254-1416.2019.11.029","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1416.2019.11.029","url":null,"abstract":"Objective \u0000To evaluate the changes in the expression of caspase recruitment domain protein 3 (NLRC3) during ventilator-induced lung injury (VILI) in rats. \u0000 \u0000 \u0000Methods \u0000Twenty-four clean-grade healthy adult male Sprague-Dawley rats, aged 8 weeks, weighing 180-220 g, were divided into 2 groups (n=12 each) according to the random number table method: control group (group C) and group VILI.Both groups underwent tracheotomy and intubation, group C kept spontaneous breathing, and the animals were mechanically ventilated, with tidal volume 20 ml/kg, respiratory rate 80 breaths/min, inspiratory/expiratory ratio 1∶1, fraction of inspired oxygen 21%, and positive end-expiratory pressure 0 in group VILI.The rats were sacrificed 4 h later, bronchoalveolar lavage fluid (BALF) was collected for determination of concentrations of interleukin-1beta (IL-1β)and IL-18 in BALF (by enzyme-linked immunosorbent assay), and lung tissues were obtained for examination of the pathological changes which were scored and for determination of wet to dry weight ratio (W/D ratio) and expression of nucleotide binding oligomerization domain-like receptor family caspase recruitment domain containing 3 (NLRC3), NOD-like receptor pyrin domain containing 3 (NLRP3), ASC and caspase-1 protein and mRNA (by Western blot and real-time polymerase chain reaction). \u0000 \u0000 \u0000Results \u0000Compared with group C, the score of pathological damage to lung tissues, W/D ratio, and concentrations of IL-1β and IL-18 in BALF were significantly increased, the expression of NLRP3, ASC and caspase-1 protein and mRNA in lung tissues was up-regulated, and the expression of NLRC3 protein and mRNA in lung tissues was down-regulated in group VILI (P<0.05). \u0000 \u0000 \u0000Conclusion \u0000The mechanism of VILI may be related to down-regulating NLRC3 expression and activating NLRP3 inflammasomes in rats. \u0000 \u0000 \u0000Key words: \u0000Ventilator-induced lung injury; NLR family, pyrin domain-containing 3 protein","PeriodicalId":10053,"journal":{"name":"中华麻醉学杂志","volume":"39 1","pages":"1387-1390"},"PeriodicalIF":0.0,"publicationDate":"2019-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48969677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of oxycodone hydrochloride controlled-release tablets for titration in treating moderate to severe cancer pain: a prospective, multicenter, randomized, parallel, controlled study","authors":"Min-min Zhu, Xiao Zhang, L. Xiao, Guo Zhen, Li Zhang, G. Xie, Shiying Yu, Ya-Qin Wang, Jianguo Xu","doi":"10.3760/CMA.J.ISSN.0254-1416.2019.11.014","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1416.2019.11.014","url":null,"abstract":"Objective \u0000To evaluate the effect of oxycodone hydrochloride controlled-release tablets for titration in treating moderate to severe cancer pain in a prospective, multicenter, randomized, parallel, controlled study. \u0000 \u0000 \u0000Methods \u0000Hospitalized cancer patients with moderate to severe pain, aged 18-64 yr, weighing 48-69 kg, with numerical rating scale(NRS)score ≥ 4 points, were divided into 4 groups according to the multicenter stratified block randomization: naive IR group, naive CR group, tolerant IR group, and tolerant CR group.A 72-h rapid titration protocol was adopted, the initial dosage: immediate-release morphine tablets 10 mg in naive IR group, oxycodone hydrochloride controlled-release tablets 10 mg in naive CR group, and 10% of the total equivalent dosage of immediate-release morphine tablets (total opioids dosage) used the day before oral administration in tolerant IR group, 10% of the total equivalent dosage of oxycodone hydrochloride controlled-release tablets (total opioids dosage)used the day before oral administration in tolerant CR group.Titration method: pain assessment was performed every hour after administration until patients went to bed; when the NRS score was increased and ≥7 points, the equivalent immediate-release morphine tablet dose was increased by 100% than the previous dose; when the NRS score was not increased but still ≥7 points, the equivalent immediate-release morphine tablet dose was increased by 50% than the previous dose; when the NRS score was 4-6 points, the equivalent immediate-release morphine tablet dose was the same as before; when the NRS score was decreased and ≤3 points, patients were continuously observed without additional medication.Maintenance method: after the end of titration, the total amount of immediate-release morphine tablets for titration was converted into an equivalent dose of oxycodone hydrochloride controlled-release tablets and was given once a day for 2 days in naive IR and tolerant IR groups; the initial dose of oxycodone hydrochloride controlled-release tablets plus the total amount of immediate-release morphine tablets for titration was converted into an equivalent dose of oxycodone hydrochloride controlled-release tablets and was given once a day for 2 days in naive CR group and tolerant CR group.When break-through pain occurred, immediate release morphine tablets 5-10 mg was taken orally to record administration, degree of pain relief, dose of oxycodone hydrochloride controlled-release tablets used, titration cycle and amount, and the effective analgesia and adverse reactions were recorded. \u0000 \u0000 \u0000Results \u0000Compared with naive IR group, the titration cycle and total titration dose were significantly reduced, the rate of effective analgesia was increased, the degree of pain relief was better, the frequency of oral morphine tablets was reduced, and the dosage of oxycodone hydrochloride controlled-release tablets used was decreased in naive CR group (P 0.05). \u0000 \u0000 \u0000Conclusion \u0000Oxycodone","PeriodicalId":10053,"journal":{"name":"中华麻醉学杂志","volume":"39 1","pages":"1330-1333"},"PeriodicalIF":0.0,"publicationDate":"2019-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47271768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of dexmedetomidine on necroptosis during intestinal injury in rats undergoing autologous orthotopic liver transplantation","authors":"Yong-wang Wang, Qingping Wang, Gang Wang, Weihua Liu, H. Du, Wenli Yu, Yonghao Yu","doi":"10.3760/CMA.J.ISSN.0254-1416.2019.11.006","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1416.2019.11.006","url":null,"abstract":"Objective \u0000To evaluate the effect of dexmedetomidine on necroptosis during intestinal injury in rats undergoing autologous orthotopic liver transplantation (AOLT). \u0000 \u0000 \u0000Methods \u0000Twenty-four SPF adult male Sprague-Dawley rats, aged 8-10 weeks, weighing 250-280 g, were divided into 3 groups (n=8 each) using a random number table method: sham operation group (group S), AOLT group (group T) and dexmedetomidine group (group D). Dexmedetomidine 50 μg/kg was intraperitoneally injected at 30 min before surgery in group D. Blood samples were collected from the inferior vena cava at 6 h after opening the hepatic portal vein (at 6 h after the end of surgery in group S) for determination of serum diamine oxidase (DAO), D-lactic acid (D-LA) and tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) concentrations.The intestine was removed for examination of the pathological changes (with a light microscope) and for determination of the level of malondialdehyde (MDA) content and superoxide dismutase (SOD) activity (using spectrophotometry) and expression of receptor-interacting protein kinase-1 (RIPK1), RIPK3, and mixed lineage kinase domain-like (MLKL) in intestinal tissues (by Western blot). Intestinal damage was assessed and scored according to Chiu. \u0000 \u0000 \u0000Results \u0000Compared with group S, the serum DAO, D-LA, TNF-α and IL-10 concentrations, intestinal MDA content and Chiu′s score were significantly increased, the SOD activity was decreased, and the expression of RIPK1, RIPK3 and MLKL was up-regulated in group T (P<0.05). Compared with group T, the serum DAO, D-LA and TNF-α concentrations, intestinal MDA content and Chiu′s score were significantly decreased, the SOD activity and serum IL-10 concentration were increased, and the expression of RIPK1, RIPK3 and MLKL was down-regulated in group D (P<0.05). \u0000 \u0000 \u0000Conclusion \u0000The mechanism by which dexmedetomidine attenuates intestinal injury is related to inhibiting necroptosis in rats undergoing AOLT. \u0000 \u0000 \u0000Key words: \u0000Dexmedetomidine; Liver transplantation; Intestine; Necrosis","PeriodicalId":10053,"journal":{"name":"中华麻醉学杂志","volume":"39 1","pages":"1298-1301"},"PeriodicalIF":0.0,"publicationDate":"2019-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44787439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}