Human blood cells (erythrocytes) were imaged by the digital holographic interference microscope (DHIM) without any preparation. DHIM represents an effective technique for 3D imaging of phase microscopic objects and measurement of their morphological parameters. It has been detected that, in addition to hematological diseases, the diseases of different genesis and external factors serve as the reason for the morphological modification of blood erythrocytes.
{"title":"Holographic Method for Blood Cell Imaging","authors":"T. Tishko, D. Tishko, V. Titar","doi":"10.1002/IMIC.200990063","DOIUrl":"https://doi.org/10.1002/IMIC.200990063","url":null,"abstract":"Human blood cells (erythrocytes) were imaged by the digital holographic interference microscope (DHIM) without any preparation. DHIM represents an effective technique for 3D imaging of phase microscopic objects and measurement of their morphological parameters. It has been detected that, in addition to hematological diseases, the diseases of different genesis and external factors serve as the reason for the morphological modification of blood erythrocytes.","PeriodicalId":100658,"journal":{"name":"Imaging & Microscopy","volume":"136 1","pages":"46-48"},"PeriodicalIF":0.0,"publicationDate":"2009-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84365464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A critical step in automated microscopy is the autofocusing algorithm. When focusing multiple fields of view, the variation of the focus plane in neighboring fields is low, and a subset of these can be used for focusing, estimating the rest by interpolation. The choice of the sampling points has an influence on the interpolation error. We introduce and test a new sampling algorithm based on the Halton set, which significantly improves the interpolation error.
{"title":"Automatic Focusing Using Halton Sampling","authors":"T. Pengo","doi":"10.1002/IMIC.200990060","DOIUrl":"https://doi.org/10.1002/IMIC.200990060","url":null,"abstract":"A critical step in automated microscopy is the autofocusing algorithm. When focusing multiple fields of view, the variation of the focus plane in neighboring fields is low, and a subset of these can be used for focusing, estimating the rest by interpolation. The choice of the sampling points has an influence on the interpolation error. We introduce and test a new sampling algorithm based on the Halton set, which significantly improves the interpolation error.","PeriodicalId":100658,"journal":{"name":"Imaging & Microscopy","volume":"2 1","pages":"39-41"},"PeriodicalIF":0.0,"publicationDate":"2009-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86625223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Carl Zeiss will launch a new product line for high resolution microscopy this year. Thanks to the combination of two techniques in Zeiss microscopes – HR-SIM (High Resolution Structured Illumination Microscopy) and PAL-M (Photoactivated Localization Microscopy) – biomedical scientists are able to examine objects at maximum resolution.
{"title":"High Resolution Microscopy","authors":"U. Simon","doi":"10.1002/IMIC.200990064","DOIUrl":"https://doi.org/10.1002/IMIC.200990064","url":null,"abstract":"Carl Zeiss will launch a new product line for high resolution microscopy this year. Thanks to the combination of two techniques in Zeiss microscopes – HR-SIM (High Resolution Structured Illumination Microscopy) and PAL-M (Photoactivated Localization Microscopy) – biomedical scientists are able to examine objects at maximum resolution.","PeriodicalId":100658,"journal":{"name":"Imaging & Microscopy","volume":"37 1","pages":"49-49"},"PeriodicalIF":0.0,"publicationDate":"2009-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75392695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Most processes in the eukaryotic cell nucleus are spatially and temporally regulated. To understand the dynamics in the underlying networks in quantitative terms necessitates the employment of technologies that can measure molecular concentrations, interactions and diffusion of proteins with high resolution. Using a combination of the available toolkit offers the potential to get a full picture of the dynamics of nuclear proteins in their most natural setting, the living cell.
{"title":"Multiscale Dynamics Assessment","authors":"P. Hemmerich, K. Weißhart","doi":"10.1002/IMIC.200990061","DOIUrl":"https://doi.org/10.1002/IMIC.200990061","url":null,"abstract":"Most processes in the eukaryotic cell nucleus are spatially and temporally regulated. To understand the dynamics in the underlying networks in quantitative terms necessitates the employment of technologies that can measure molecular concentrations, interactions and diffusion of proteins with high resolution. Using a combination of the available toolkit offers the potential to get a full picture of the dynamics of nuclear proteins in their most natural setting, the living cell.","PeriodicalId":100658,"journal":{"name":"Imaging & Microscopy","volume":"21 1","pages":"42-45"},"PeriodicalIF":0.0,"publicationDate":"2009-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74175305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This newsletter will appear in the I&M issue that will also be distributed at the 2009 EMS Extension at the MC2009 congress, the 9th Multinational Congress on Microscopy and the Dreilandertagung, taking place in Graz, Austria, from August 30 till September 4 of this year. From the website at http://www.microscopy09.tugraz.at/, which is constantly being updated, it is clear that the preparations are going well and are promising a very exciting meeting with sessions on all major and up-to-date topics in various fields of modern microscopy. After a broad call, EMS received 23 applications for scholarships for young researchers. Of these, 18 were selected and will receive sponsorship of 250 euro to support their participation at MC2009. So please, do look for the EMS logo on posters and lecture slides!
{"title":"EMS Newsletter 27, July 2009","authors":"D. Schryvers","doi":"10.1002/IMIC.200990047","DOIUrl":"https://doi.org/10.1002/IMIC.200990047","url":null,"abstract":"This newsletter will appear in the I&M issue that will also be distributed at the 2009 EMS Extension at the MC2009 congress, the 9th Multinational Congress on Microscopy and the Dreilandertagung, taking place in Graz, Austria, from August 30 till September 4 of this year. From the website at http://www.microscopy09.tugraz.at/, which is constantly being updated, it is clear that the preparations are going well and are promising a very exciting meeting with sessions on all major and up-to-date topics in various fields of modern microscopy. After a broad call, EMS received 23 applications for scholarships for young researchers. Of these, 18 were selected and will receive sponsorship of 250 euro to support their participation at MC2009. So please, do look for the EMS logo on posters and lecture slides!","PeriodicalId":100658,"journal":{"name":"Imaging & Microscopy","volume":"168 1","pages":"12-12"},"PeriodicalIF":0.0,"publicationDate":"2009-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83918033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In recent years there has been an ongoing trend to design specialized scanning electron microscopes, dedicated to specific applications. As a result two different system configurations in the SEM market have emerged: One configuration is dedicated to deliver high resolution imaging. The other configuration is dedicated to optimized analytical performance. However, these two diverging development lines force users to decide whether they will limit their research to high resolution imaging or optimized analytical investigations. With the introduction of the new Merlin, Carl Zeiss now has launched an optimized solution fulfilling the requirements for both: the high resolution and the analytical needs. Its imaging system attaining a maximum secondary electron detection resolution of 0.8 nm combines a wealth of analytical capabilities based on a maximum probe current of 300 nA.
{"title":"Analytical Power for the Sub‐Nanometer World","authors":"M. Wiederspahn","doi":"10.1002/IMIC.200990055","DOIUrl":"https://doi.org/10.1002/IMIC.200990055","url":null,"abstract":"In recent years there has been an ongoing trend to design specialized scanning electron microscopes, dedicated to specific applications. As a result two different system configurations in the SEM market have emerged: One configuration is dedicated to deliver high resolution imaging. The other configuration is dedicated to optimized analytical performance. However, these two diverging development lines force users to decide whether they will limit their research to high resolution imaging or optimized analytical investigations. With the introduction of the new Merlin, Carl Zeiss now has launched an optimized solution fulfilling the requirements for both: the high resolution and the analytical needs. Its imaging system attaining a maximum secondary electron detection resolution of 0.8 nm combines a wealth of analytical capabilities based on a maximum probe current of 300 nA.","PeriodicalId":100658,"journal":{"name":"Imaging & Microscopy","volume":"11 1","pages":"25-26"},"PeriodicalIF":0.0,"publicationDate":"2009-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75335305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"TIRF: Using Evanescent Waves for High Resolution Membrane Fluorescence Imaging","authors":"S. Treves, F. Zorzato","doi":"10.1002/IMIC.200990065","DOIUrl":"https://doi.org/10.1002/IMIC.200990065","url":null,"abstract":"","PeriodicalId":100658,"journal":{"name":"Imaging & Microscopy","volume":"117 10 1","pages":"52-53"},"PeriodicalIF":0.0,"publicationDate":"2009-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84257235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this number of the EMS newsletter we have 4 major issues. First of all we would very much like to welcome Prof. Dr. Christian Schofer from the Medical University of Vienna, Austria, as the new Treasurer of EMS, taking over the task of Prof. Dr. Leo Ginsel who passed away last January. The search for a new Treasurer was not easy but we are convinced that we have found a very reliable and conscientious colleague in Christian. All relevant paperwork that was left by Leo and his co-worker Jack Theuws is now with Christian, the info for the bank account has been transferred (but the account number and bank haven't changed!), and Christian is ready to come and to join us on the EMS Executive Board.
{"title":"EMS Newsletter 26, April 2009","authors":"C. Schöfer","doi":"10.1002/IMIC.200990023","DOIUrl":"https://doi.org/10.1002/IMIC.200990023","url":null,"abstract":"In this number of the EMS newsletter we have 4 major issues. First of all we would very much like to welcome Prof. Dr. Christian Schofer from the Medical University of Vienna, Austria, as the new Treasurer of EMS, taking over the task of Prof. Dr. Leo Ginsel who passed away last January. The search for a new Treasurer was not easy but we are convinced that we have found a very reliable and conscientious colleague in Christian. All relevant paperwork that was left by Leo and his co-worker Jack Theuws is now with Christian, the info for the bank account has been transferred (but the account number and bank haven't changed!), and Christian is ready to come and to join us on the EMS Executive Board.","PeriodicalId":100658,"journal":{"name":"Imaging & Microscopy","volume":"23 1","pages":"10-10"},"PeriodicalIF":0.0,"publicationDate":"2009-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74916667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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