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The same-stage reduction plasty for labia minora hypertrophy with redundant praeputium clitoridis 小阴唇肥大伴阴蒂前突肥大的同期整复术
Pub Date : 2019-06-15 DOI: 10.3760/CMA.J.ISSN.1671-0290.2019.03.016
Yulin Dong, Liwei Dong, Hao Dongyue, Wensen Xia
Objective To evaluate the clinical outcome of reduction plasty for labia minora hypertrophy with redundant praeputium clitoridis. Methods A total of 84 cases were included from June 2013 to December 2016. Arc-shaped resection of labia minora and crescent resection of redundant praeputium clitoridis were the two essntial surgical procedure, and 5-0 VICRYL Rapide was used for suturing the incision. Results The 84 cases were operated with this mothod with satisfactory aesthetic result. Complications, such as hematoma, infection and poor wound healing, were not observed. Edema was resoluted after one to two weeks. After the reduction plasty of the labial labia, the vagina and perineum were completely covered in the natural state, without scar, also the sensitivity of clitoris was improved due to the better expoure post-operationally. Conclusions The same-stage reduction plasty for labia minora hypertrophy with redundant praeputium clitoridis is a reliable and effective method. Both satisfactory aesthetic result and improved sexual sensitivity are achived, without hematoma, infection and poor wound healing. Key words: Clitoris; Reconstructive surgical procedures; Labia minora hypertrophy; Reduction plasty
目的探讨小阴唇肥大伴阴蒂前体过厚的临床效果。方法选取2013年6月至2016年12月收治的84例患者。小阴唇弧形切除和月牙形切除多余阴蒂前体是两种必要的手术方式,切口采用5-0 VICRYL Rapide缝合。结果84例手术均获得满意的美观效果。未见血肿、感染、伤口愈合不良等并发症。水肿在一至两周后消退。阴唇缩小成形术后,阴道和会阴完全覆盖在自然状态下,无瘢痕,阴蒂也因术后更好的暴露而提高了敏感性。结论小阴唇肥大伴阴蒂前体赘肉同期缩小成形术是一种可靠、有效的治疗方法。既达到满意的审美效果,又提高了性敏感性,无血肿、感染和伤口愈合不良。关键词:阴蒂;重建外科手术;小阴唇肥大;减少成形术
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引用次数: 0
Clinical effect of combined bandage contact lenses and artificial tears on corneal epithelial injury due to correction of blepharoptosis 绷带隐形眼镜联合人工泪液治疗上睑下垂矫正术后角膜上皮损伤的临床观察
Pub Date : 2019-06-15 DOI: 10.3760/CMA.J.ISSN.1671-0290.2019.03.003
Wang Jingxian, Yang Yuan, Liu Wenying
Objective To explore the effect of the combination of bandage contact lenses and artificial tears on corneal epithelial injury, due to correction of blepharoptosis in patients. Methods Thirty-five patients (56 eyes) were included in this randomized controlled trial during May 2013 to May 2018, and randomly divided into observation group (combination of bandage contact lenses and artificial tears) and control group (combination of erythromycin eye ointment and artificial tears), and followed up for 3 months. The corneal wound healing, corneal fluorescent staining (FL), Schirmer test (Slt), break-up time (BUT) and the severity of eye pain were documented and compared by statistical method. Results The scores of FL in the observation group were reduced (t=-3.211, P 0.05; the scores of eye pain, t=-1.800, P>0.05; the corneal wound healing, χ2=1.018, P>0.05; the BUT score, t=1.661, P>0.05). Conclusions The combination of bandage contact lenses and artificial tears is recommended for the early use after correction of blepharoptosis, to alleviate eye symptoms and promote corneal wound healing. Key words: Blepharoptosis; Corneal injuries; Ptosis; Corneal epithelial injury; Contact lens
目的探讨带式隐形眼镜联合人工泪液治疗上睑下垂患者角膜上皮损伤的疗效。方法2013年5月至2018年5月,本随机对照试验纳入35例(56眼)患者,随机分为观察组(绷带隐形眼镜与人工泪液联合使用)和对照组(红霉素眼膏与人工泪剂联合使用),随访3个月。记录角膜伤口愈合、角膜荧光染色(FL)、Schirmer试验(Slt)、破裂时间(BUT)和眼痛的严重程度,并用统计学方法进行比较。结果观察组FL评分降低(t=-3.211,P<0.05;眼痛评分t=-1.800,P>0.05;角膜创伤愈合率χ2=1.018,P>0.01;BUT评分t=1.661,P<0.01),以减轻眼部症状并促进角膜伤口愈合。关键词:眼睑下垂;角膜损伤;Ptosis;角膜上皮损伤;隐形眼镜
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引用次数: 0
Clinical effects of nasal glabellar flap on reconstruction of nasal dorsal and tip 鼻眉间皮瓣在鼻背侧和鼻尖重建中的临床应用
Pub Date : 2019-04-15 DOI: 10.3760/CMA.J.ISSN.1671-0290.2019.02.010
Xin Su, Fangzhu Zhou
Objective To introduce an excellent one-stage procedure for repairing defects of the middle and distal part of the nose: nasal dorsal glabellar flap. Methods According to the size, shape and location of nasal defect, homologous nasal dorsal glabellar flap was selected to cover the nasal defect in 17 cases. Among 17 cases, there were 7 men and 10 women, with age of 38 to 76 years. The locations were nasal tip in 9 cases and dorsum of nose in 8 cases, The area of the defect ranged from 15 mm×15 mm to 30 mm×26 mm. The nasal dorsal glabellar flap, which received a versatile axial blood supply from branches of the facial artery and ophthalmic artery, took tissue from the lax glabellar skin and might appear to be too aggressive for closure of a distal nasal or mid-nasal defect. However, because its design was primarily that of a rotation flap with a back-cut in the glabella region, it was greater than the primary defect in order to maximize tissue movement and decreased wound closure tension at the flap donor site. The secondary defect of donor site was directly sutured. Results The nasal defect was successfully repaired in all patients, and the all flaps survived. The patients were available for follow-up of 1 to 6 months, no tumor recurrence occurred, and the repaired tissue were good match with surrounding tissue in color, thickness and texture, good nasal contour was obtained and no secondary deformity occurred, and therefore the cosmetic results were satisfactory. Conclusions The nasal glabellar flap has evolved into a choice flap for nasal defects of cover since it provides local skin with an exact color, thickness, and texture match for the nasal skin. It allows large reconstructions for up to 30 mm defects leaving minimal scars. It represents an interesting alternative for the reconstruction of defects of the nasal tip or supra tip of the nose. Key words: Nose; Skin; Reconstructive surgical procedures; Surgical flaps; Nasal glabellar flap; Defects
目的介绍一种修复鼻中、远端缺损的一期手术方法:鼻背侧眉间皮瓣。方法根据鼻缺损的大小、形状和位置,选择相应的鼻眉间背侧皮瓣修复鼻缺损17例。在17例病例中,有7名男性和10名女性,年龄在38至76岁之间。位置为鼻尖9例,鼻背8例,缺损面积为15mm×15mm至30mm×26mm,从松弛的眉间皮肤上取下组织,可能看起来过于激进,无法闭合鼻远端或中鼻缺损。然而,由于其设计主要是在眉间区域有背部切口的旋转皮瓣,因此它比原发性缺损更大,以最大限度地增加组织运动并降低皮瓣供区的伤口闭合张力。供区继发性缺损直接缝合。结果所有患者均成功修复鼻腔缺损,皮瓣全部成活。患者随访1~6个月,无肿瘤复发,修复后的组织在颜色、厚度和质地上与周围组织匹配良好,鼻腔轮廓良好,无继发畸形,美容效果满意。结论鼻眉间皮瓣为局部皮肤提供了与鼻腔皮肤完全匹配的颜色、厚度和质地,已成为治疗鼻腔覆盖缺损的首选皮瓣。它允许对多达30毫米的缺陷进行大型重建,留下最小的疤痕。它代表了重建鼻尖或鼻尖上缺损的一种有趣的替代方案。关键词:鼻子;皮肤;重建外科手术;外科皮瓣;鼻眉间皮瓣;缺陷
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引用次数: 0
Application of fat redistribution combined with shortening of upper eyelid levator muscle in repair of wide double-eyelid 脂肪再分配联合上睑提肌缩短术在宽双眼皮修复中的应用
Pub Date : 2019-04-15 DOI: 10.3760/CMA.J.ISSN.1671-0290.2019.02.016
An-li Zhang, Ze-Chun Huang
Objective To evaluate the clinical efficacy of topical fat redistribution in combination with shortening of upper eyelid levator muscle in patients with excessively wide palpebral fissure after double-eyelid operation. Methods Twenty patients with excessively wide palpebral fissure after undergoing double-eyelid operation were selected. The double-eyelid line was aesthetically re-designed, the skin or incisional scar between the original and new double-eyelid lines was excised, and the scarring adhesion among different tissues was fully isolated. The fat tissues within the orbital septum, deep orbit and anterior orbital septum combined with ROOF reconstruction were performed to block the adhesion tissues, shortening of levator muscle of the upper eyelid was equally conducted to enhance the cosmetic effect. Results Seventeen patients were satisfied with the cosmetic appearance following once double-eyelid operation. Two cases underwent twice operations due to different height between bilateral papebral fissures. One patient was unsatisfied with the cosmetic appearance. Conclusions Fat redistribution in combination with shortening of levator muscle of the upper eyelid can not only repair excessively wide palpebral fissure complicated with ptosis and upper eyelid depression, but also enhance the exposure rate of the cornea and increase the cosmetic appearance, which is consistent with contemporary aesthetic perception. Key words: Blepharoplasty; Reconstructive surgical procedures; Construction of double eyelid; Repair; Shortening of upper eyelid levator muscle
目的探讨局部脂肪重分布联合上睑提肌缩短术治疗睑裂过宽双眼皮术后患者的临床疗效。方法选择20例双眼皮术后睑裂过宽患者。对双眼皮线进行美学重新设计,切除原双眼皮线与新双眼皮线之间的皮肤或切口疤痕,充分隔离不同组织间的瘢痕粘连。眶隔内、眶深、眶前隔脂肪组织联合ROOF重建,阻断粘连组织,同时缩短上睑提上肌,增强美容效果。结果17例患者一次双眼皮手术后外观满意。2例因双侧睑裂高度不同,接受两次手术。一名患者对美容外观不满意。结论脂肪重分布联合提上睑肌缩短术不仅能修复睑裂过宽并发上睑下垂、上睑凹陷,而且能提高角膜暴露率,增加美观度,符合当代审美观念。关键词:眼睑成形术;重建外科手术;双眼皮构造;修理;上睑提肌缩短
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引用次数: 0
Changes of macrophages phenotype markers in fibrous capsules around silicone implants 硅胶植入物周围纤维囊巨噬细胞表型标志物的变化
Pub Date : 2019-04-15 DOI: 10.3760/CMA.J.ISSN.1671-0290.2019.02.001
Jianke Ding, L. Lei, Zhou Yu, Yingjun Su
Objective To study the temporal distribution of macrophage and its phenotype markers in fibrous capsules around silicone implants. Methods Thirty rats were randomly divided into five groups: days 1, 3, 7, 14 and 35. Silicone prostheses (10 ml) were implanted subcutaneously into backs of rats. On each indicated day, the tissue specimens were collected, fixed in 4% paraformaldehyde for 24 hours and embedded in paraffin. Immunofluorescence was used to detect temporal distribution of M1/M2 macrophages. Results The number of CD68+ macrophages at day 1 (65.8±12.9) was smaller than that at day 3 (102.8±14.5, P<0.05) and day 7 (116.8±14.2, P<0.05); and the number of CD68+ macrophages at day 7 was larger than that at day 14 (56.8±12.9, P<0.05) and day 35 (21.40±6.35, P<0.05); the proportion of iNOS+ CD68+ M1 cells at day 1 and day 3 was 0.48±0.13, 0.60±0.13, respectively, and they were higher than that at day 7 (0.21±0.03, P<0.05), day 14 (0.21±0.03, P<0.05) and day 35 (0.17±0.04, P<0.05); the proportions of CD206+ CD68+ M2 cells at day 1, day 3, day 7, day 14, day 35 were 0.70±0.06, 0.60±0.07, 0.70±0.08, 0.67±0.02 and 0.60±0.06, respectively. Conclusions After the implantation of silicone prostheses, M1 cells increase in early stages and M2 cells maintain in high level throughout the experiment period. Key words: Macrophages; Silica gel; Prosthesis implantation; Implant capsular contracture; Rats
目的研究硅胶植入物周围纤维囊巨噬细胞的时间分布及其表型标志物。方法30只大鼠随机分为5组:第1、3、7、14和35天。将硅胶假体(10ml)皮下植入大鼠背部。在每个指定的日子,收集组织标本,在4%多聚甲醛中固定24小时,并包埋在石蜡中。免疫荧光法检测M1/M2巨噬细胞的时间分布。结果CD68+巨噬细胞在第1天(65.8±12.9)少于第3天(102.8±14.5,P<0.05)和第7天(116.8±14.2,P<0.05);CD68+巨噬细胞在第7天的数量大于第14天(56.8±12.9,P<0.05)和第35天(21.40±6.35,P<0.05);iNOS+CD68+M1细胞在第1天和第3天的比例分别为0.48±0.13、0.60±0.13,高于第7天(0.21±0.03,P<0.05)、第14天(0.211±0.03,P<0.01)和第35天(0.17±0.04,P<0.05);CD206+CD68+M2细胞在第1天、第3天、第7天、第14天和第35天的比例分别为0.70±0.06、0.60±0.07、0.70±0.08、0.67±0.02和0.60±0.06。结论硅胶假体植入后,M1细胞在早期增加,M2细胞在整个实验期间保持高水平。关键词:巨噬细胞;硅胶;假体植入;种植体包膜挛缩;大鼠
{"title":"Changes of macrophages phenotype markers in fibrous capsules around silicone implants","authors":"Jianke Ding, L. Lei, Zhou Yu, Yingjun Su","doi":"10.3760/CMA.J.ISSN.1671-0290.2019.02.001","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1671-0290.2019.02.001","url":null,"abstract":"Objective \u0000To study the temporal distribution of macrophage and its phenotype markers in fibrous capsules around silicone implants. \u0000 \u0000 \u0000Methods \u0000Thirty rats were randomly divided into five groups: days 1, 3, 7, 14 and 35. Silicone prostheses (10 ml) were implanted subcutaneously into backs of rats. On each indicated day, the tissue specimens were collected, fixed in 4% paraformaldehyde for 24 hours and embedded in paraffin. Immunofluorescence was used to detect temporal distribution of M1/M2 macrophages. \u0000 \u0000 \u0000Results \u0000The number of CD68+ macrophages at day 1 (65.8±12.9) was smaller than that at day 3 (102.8±14.5, P<0.05) and day 7 (116.8±14.2, P<0.05); and the number of CD68+ macrophages at day 7 was larger than that at day 14 (56.8±12.9, P<0.05) and day 35 (21.40±6.35, P<0.05); the proportion of iNOS+ CD68+ M1 cells at day 1 and day 3 was 0.48±0.13, 0.60±0.13, respectively, and they were higher than that at day 7 (0.21±0.03, P<0.05), day 14 (0.21±0.03, P<0.05) and day 35 (0.17±0.04, P<0.05); the proportions of CD206+ CD68+ M2 cells at day 1, day 3, day 7, day 14, day 35 were 0.70±0.06, 0.60±0.07, 0.70±0.08, 0.67±0.02 and 0.60±0.06, respectively. \u0000 \u0000 \u0000Conclusions \u0000After the implantation of silicone prostheses, M1 cells increase in early stages and M2 cells maintain in high level throughout the experiment period. \u0000 \u0000 \u0000Key words: \u0000Macrophages; Silica gel; Prosthesis implantation; Implant capsular contracture; Rats","PeriodicalId":10094,"journal":{"name":"Chinese Journal of Medical Aesthetics and Cosmetology","volume":"25 1","pages":"171-175"},"PeriodicalIF":0.0,"publicationDate":"2019-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45716422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Efficacy of 308 nm monochromatic excimer light and 311 nm narrow band ultraviolet on facial vitiligo 308nm单色准分子光和311nm窄带紫外线治疗面部白癜风疗效观察
Pub Date : 2019-04-15 DOI: 10.3760/CMA.J.ISSN.1671-0290.2019.02.018
Xuehong Yu
Objective To compare the efficacy of monochromatic excimer light and narrow-band ultraviolet in the treatment of facial vitiligo patients. Methods Seventy-nine patients with facial vitiligo were randomly divided into MEL group and NB-UVB group. The MEL and 311-nm NB-UVB were applied respectively. Both groups were treated twice a week, total 30 times. Results After ten times treatment, the effective rate of MEL group and NB-UVB group was 39.02% and 15.79%, respectively. There was significant difference between the two groups (P 0.05). The efficacy of both groups showed that disease duration was a negative factor. It seemed that shorter duration of facial vitiligo would obtain better clinical effect. MEL and NB-UVB were safe and effective for the progressive vitiligo. In both groups, few adverse effets in some patients were observed. Conclusions The clinical effect of MEL in treating facial vitiligo is more rapidly than NB-UVB, but as the treatment times increase, the efficacy becomes similar. Both methods are safe in treating facial vitiligo of different stages. Key words: Vitiligo; Face; 308 nm monochromatic excimer light; Ultraviolet B, narrow-band
目的比较单色准分子光与窄带紫外线治疗面部白癜风的疗效。方法79例面部白癜风患者随机分为MEL组和NB-UVB组。分别采用MEL和311 nm NB-UVB。两组患者每周治疗2次,共30次。结果经10次治疗后,MEL组和NB-UVB组的有效率分别为39.02%和15.79%。两组比较差异有统计学意义(p0.05)。两组疗效均显示病程为负向因素。面部白癜风持续时间越短,临床效果越好。MEL和NB-UVB治疗进行性白癜风安全有效。两组患者均未见不良反应。结论MEL治疗面部白癜风的临床疗效快于NB-UVB,但随着治疗次数的增加,两者疗效相近。两种方法治疗不同阶段面部白癜风均安全。关键词:白癜风;脸;308 nm单色准分子光;紫外线B,窄带
{"title":"Efficacy of 308 nm monochromatic excimer light and 311 nm narrow band ultraviolet on facial vitiligo","authors":"Xuehong Yu","doi":"10.3760/CMA.J.ISSN.1671-0290.2019.02.018","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1671-0290.2019.02.018","url":null,"abstract":"Objective \u0000To compare the efficacy of monochromatic excimer light and narrow-band ultraviolet in the treatment of facial vitiligo patients. \u0000 \u0000 \u0000Methods \u0000Seventy-nine patients with facial vitiligo were randomly divided into MEL group and NB-UVB group. The MEL and 311-nm NB-UVB were applied respectively. Both groups were treated twice a week, total 30 times. \u0000 \u0000 \u0000Results \u0000After ten times treatment, the effective rate of MEL group and NB-UVB group was 39.02% and 15.79%, respectively. There was significant difference between the two groups (P 0.05). The efficacy of both groups showed that disease duration was a negative factor. It seemed that shorter duration of facial vitiligo would obtain better clinical effect. MEL and NB-UVB were safe and effective for the progressive vitiligo. In both groups, few adverse effets in some patients were observed. \u0000 \u0000 \u0000Conclusions \u0000The clinical effect of MEL in treating facial vitiligo is more rapidly than NB-UVB, but as the treatment times increase, the efficacy becomes similar. Both methods are safe in treating facial vitiligo of different stages. \u0000 \u0000 \u0000Key words: \u0000Vitiligo; Face; 308 nm monochromatic excimer light; Ultraviolet B, narrow-band","PeriodicalId":10094,"journal":{"name":"Chinese Journal of Medical Aesthetics and Cosmetology","volume":"25 1","pages":"233-236"},"PeriodicalIF":0.0,"publicationDate":"2019-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41541639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Repair of extremity scar contracture by expanded local flaps 扩张局部皮瓣修复四肢瘢痕挛缩
Pub Date : 2019-04-15 DOI: 10.3760/CMA.J.ISSN.1671-0290.2019.02.012
Xianjie Ma, Yang Li, Hengxin Liu, Liwei Dong
Objective To investigate the application of expanded local flap in repairing extremity scars. Methods Thirty patients with extremity scars contracture as a result of trauma, surgery or infection during July 2000 and March 2013 were included. There were 11 male and 19 female patients with age ranging from 11 to 34 years. Tissue expansion, scar resection, flap transfer and scar management were involved in this process. Results There 24 patients suffered from upper extremity scars and 6 patients suffered from lower extremity scars. Expander exposure was noted in 3 patients, and 3 patients suffered from infection during the expansion. During the 6-month to 1-year follow-up, all patients satisfied with the results in terms of the skin texture, color, flexibility after the treatment with expanded local skin flap. Conclusions The expanded local flaps are suitable for the repair of the longitudinal and oblique scars in the extremities. Key words: Cicatrix; Contracture; Extremities; Dilatation; Surgical flaps
目的探讨扩张局部皮瓣在四肢瘢痕修复中的应用。方法对2000年7月至2013年3月期间30例因外伤、手术或感染导致的四肢瘢痕挛缩症患者进行分析。共有11名男性和19名女性患者,年龄从11岁到34岁不等。该过程涉及组织扩张、瘢痕切除、皮瓣转移和瘢痕处理。结果上肢瘢痕24例,下肢瘢痕6例。3名患者出现扩张器暴露,3名患者在扩张器期间感染。在6个月至1年的随访中,所有患者都对扩张局部皮瓣治疗后的皮肤质地、颜色、柔韧性等方面的结果感到满意。结论扩张局部皮瓣适用于四肢纵、斜向瘢痕的修复。关键词:Ciatrix;合同;极端主义;扩张;外科皮瓣
{"title":"Repair of extremity scar contracture by expanded local flaps","authors":"Xianjie Ma, Yang Li, Hengxin Liu, Liwei Dong","doi":"10.3760/CMA.J.ISSN.1671-0290.2019.02.012","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1671-0290.2019.02.012","url":null,"abstract":"Objective \u0000To investigate the application of expanded local flap in repairing extremity scars. \u0000 \u0000 \u0000Methods \u0000Thirty patients with extremity scars contracture as a result of trauma, surgery or infection during July 2000 and March 2013 were included. There were 11 male and 19 female patients with age ranging from 11 to 34 years. Tissue expansion, scar resection, flap transfer and scar management were involved in this process. \u0000 \u0000 \u0000Results \u0000There 24 patients suffered from upper extremity scars and 6 patients suffered from lower extremity scars. Expander exposure was noted in 3 patients, and 3 patients suffered from infection during the expansion. During the 6-month to 1-year follow-up, all patients satisfied with the results in terms of the skin texture, color, flexibility after the treatment with expanded local skin flap. \u0000 \u0000 \u0000Conclusions \u0000The expanded local flaps are suitable for the repair of the longitudinal and oblique scars in the extremities. \u0000 \u0000 \u0000Key words: \u0000Cicatrix; Contracture; Extremities; Dilatation; Surgical flaps","PeriodicalId":10094,"journal":{"name":"Chinese Journal of Medical Aesthetics and Cosmetology","volume":"25 1","pages":"212-214"},"PeriodicalIF":0.0,"publicationDate":"2019-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49279135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of autologous platelet-rich plasma on directional induced differentiation of rabbit bone marrow mesenchymal stem cells 自体富血小板血浆对兔骨髓间充质干细胞定向诱导分化的影响
Pub Date : 2019-04-15 DOI: 10.3760/CMA.J.ISSN.1671-0290.2019.02.020
Ma Zhibing, Fengying Yuan, P. Jin, A. Zhang, Chang-bo Tao, Xue-yang Li, Caiqi Shen, Yanping Guo
Objective To study the cell morphology and differentiation efficiency when rabbit bone marrow mesenchymal stem cells (BMSCs) were induced osteogenic differentiation as culturing by autologous platelet-rich plasma (PRP) instead of serum, and to explore a new method of inducing BMSCs osteogenic differentiation. Methods The PRP was prepared by arterial blood of rabbit. Punctured and The bone marrow was sampled from rabbit's iliac bone, and BMSCs were collected, which divided into PRP group, fetal calf serum (FBS) group and serum-free control group, and cultured in 10% autologous PRP, 10% FBS and serum-free respectively, combined with DMEM-F12 medium. The second generation cells were divided into experimental and control groups. The experimental groups' medium was added dexamethasone, β-sodium glycerophosphate and ascorbic acid, and the control groups went on. The cell morphological difference of each group was Observed between anterior and after inducing differentiation, and compared between each group. Results BMSCs of PRP and FBS groups grew quickly, presented like fusiform form before induction, and increasd in volume, became a triangle, polygonal and round form gradually after osteogenic induction. Cells of PRP and FBS groups aggregated spontaneously and multilayered, and formed calcium nodules and bone-like structure after induced 7 days averagely, which could be stained red by alizarin red S; cells of serum-free groups were induced 14 days averagely, only three samples showed osteogenesis performance. Cells of PRP and FBS groups differentiation efficiency was superior to serum-free groups when inducd 20 days, the difference was statistically significant (P 0.05). Conclusions Autologous PRP could be used to proliferate and induce osteogenic differentiation of BMSCs instead of serum. Key words: Rabbits; Bone marrow; Stromal cells; Stem cells; Platelet-rich plasma; Osteoblasts; Cell Differentiation
目的研究自体富血小板血浆(PRP)代替血清诱导兔骨髓间充质干细胞(BMSCs)成骨分化时的细胞形态和分化效率,探索一种诱导BMSCs成骨分化的新方法。方法采用家兔动脉血制备PRP。取兔髂骨穿刺骨髓,采集骨髓间充质干细胞,分为PRP组、胎牛血清(FBS)组和无血清对照组,分别在10%自体PRP、10%胎牛血清和无血清中培养,并结合DMEM-F12培养基。第二代细胞分为实验组和对照组。实验组在培养液中加入地塞米松、β-甘油磷酸钠和抗坏血酸,对照组继续。观察各组细胞分化前和诱导分化后的形态差异,并比较各组间的差异。结果PRP组和FBS组骨髓间充质干细胞生长迅速,诱导前呈梭状,成骨诱导后体积逐渐增大,逐渐变成三角形、多边形和圆形。PRP组和FBS组细胞自发聚集,呈多层状,平均诱导7 d后形成钙结节和骨样结构,茜素红S染色;无血清组细胞平均诱导14 d,只有3个样本显示成骨能力。诱导20 d时,PRP组和FBS组细胞分化效率优于无血清组,差异有统计学意义(p0.05)。结论自体PRP可替代血清诱导骨髓间充质干细胞增殖和成骨分化。关键词:家兔;骨髓;基质细胞;干细胞;富含血小板血浆;成骨细胞;细胞分化
{"title":"Effect of autologous platelet-rich plasma on directional induced differentiation of rabbit bone marrow mesenchymal stem cells","authors":"Ma Zhibing, Fengying Yuan, P. Jin, A. Zhang, Chang-bo Tao, Xue-yang Li, Caiqi Shen, Yanping Guo","doi":"10.3760/CMA.J.ISSN.1671-0290.2019.02.020","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1671-0290.2019.02.020","url":null,"abstract":"Objective \u0000To study the cell morphology and differentiation efficiency when rabbit bone marrow mesenchymal stem cells (BMSCs) were induced osteogenic differentiation as culturing by autologous platelet-rich plasma (PRP) instead of serum, and to explore a new method of inducing BMSCs osteogenic differentiation. \u0000 \u0000 \u0000Methods \u0000The PRP was prepared by arterial blood of rabbit. Punctured and The bone marrow was sampled from rabbit's iliac bone, and BMSCs were collected, which divided into PRP group, fetal calf serum (FBS) group and serum-free control group, and cultured in 10% autologous PRP, 10% FBS and serum-free respectively, combined with DMEM-F12 medium. The second generation cells were divided into experimental and control groups. The experimental groups' medium was added dexamethasone, β-sodium glycerophosphate and ascorbic acid, and the control groups went on. The cell morphological difference of each group was Observed between anterior and after inducing differentiation, and compared between each group. \u0000 \u0000 \u0000Results \u0000BMSCs of PRP and FBS groups grew quickly, presented like fusiform form before induction, and increasd in volume, became a triangle, polygonal and round form gradually after osteogenic induction. Cells of PRP and FBS groups aggregated spontaneously and multilayered, and formed calcium nodules and bone-like structure after induced 7 days averagely, which could be stained red by alizarin red S; cells of serum-free groups were induced 14 days averagely, only three samples showed osteogenesis performance. Cells of PRP and FBS groups differentiation efficiency was superior to serum-free groups when inducd 20 days, the difference was statistically significant (P 0.05). \u0000 \u0000 \u0000Conclusions \u0000Autologous PRP could be used to proliferate and induce osteogenic differentiation of BMSCs instead of serum. \u0000 \u0000 \u0000Key words: \u0000Rabbits; Bone marrow; Stromal cells; Stem cells; Platelet-rich plasma; Osteoblasts; Cell Differentiation","PeriodicalId":10094,"journal":{"name":"Chinese Journal of Medical Aesthetics and Cosmetology","volume":"25 1","pages":"240-244"},"PeriodicalIF":0.0,"publicationDate":"2019-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46208133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Treatment of blepharoptosis with pseudo-Graefe phenomenon caused by oculomotor nerve paralysis: report of three cases 动眼神经麻痹引起的假性graefe现象治疗上睑下垂3例
Pub Date : 2019-04-15 DOI: 10.3760/CMA.J.ISSN.1671-0290.2019.02.007
Xinyu Huang, Xingxing Wang, Haitao Xu, Rui Tian, Yuan Y Du
Objective To explore the treatment of unilateral blepharoptosis caused by oculomotor nerve paralysis with pseudo-Graefe phenomenon after craniocerebral trauma or operation. Methods Three patients suffered from blepharoptosis caused by oculomotor nerve paralysis with pseudo-Graefe phenomenon were collected in this study and the modified levator resection technique was applied to correct ptosis. The observation index after operation included the height of upper palpebral margin, the radian of double eyelid, the change of eye movement and the corresponding upper eyelid height, the eye closure function and the incidence of exposure keratitis. Results One case of oculomotor nerve palsy with moderate ptosis achieved an ideal height of upper eyelid after surgery, two cases of oculomotor nerve palsy with severe ptosis were undercorrection. All the three patients had smooth double eyelid radian, no obvious change of eye movement and corresponding upper eyelid height compared with preoperative. The two severe ptoses could close eyes well, but the moderate ptosis patient couldnot close eyes completely. Conclusions The technique of modified levator resection is an effective method to treat the moderate ptosis caused by oculomotor nerve paralysis with pseudo-Graefe phenomenon, but the results are unfavorable in the treatment of severe ptosis. Key words: Oculomotor nerve; Paralysis; Blepharoptosis; Pseudo-Graefe phenomenon, ptosis; Modified levator resection technique
目的探讨颅脑损伤或手术后并发假性Graefe现象的动眼神经麻痹所致单侧上睑下垂的治疗方法。方法收集3例上睑下垂患者,采用改良提上肌切除术矫正上睑下垂。术后观察指标包括上睑缘高度、双眼皮弧度、眼球运动及相应上睑高度的变化、闭眼功能及暴露性角膜炎的发生率。结果1例中度上睑下垂的动眼神经麻痹患者术后上睑高度达到理想,2例重度上睑下垂患者术后矫正不足。三例患者双眼皮弧度均较术前平滑,眼球运动及相应的上眼睑高度均无明显变化。两例重度上睑下垂均能很好地闭上眼睛,而中度上睑下垂患者则不能完全闭上眼睛。结论改良提上睑肌切除术是治疗动眼神经麻痹伴假性Graefe现象的中度上睑下垂的有效方法,但对严重上睑下垂疗效不佳。关键词:眼球运动神经;麻痹;眼睑下垂;假性Graefe现象,上睑下垂;改良提肌切除术
{"title":"Treatment of blepharoptosis with pseudo-Graefe phenomenon caused by oculomotor nerve paralysis: report of three cases","authors":"Xinyu Huang, Xingxing Wang, Haitao Xu, Rui Tian, Yuan Y Du","doi":"10.3760/CMA.J.ISSN.1671-0290.2019.02.007","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1671-0290.2019.02.007","url":null,"abstract":"Objective \u0000To explore the treatment of unilateral blepharoptosis caused by oculomotor nerve paralysis with pseudo-Graefe phenomenon after craniocerebral trauma or operation. \u0000 \u0000 \u0000Methods \u0000Three patients suffered from blepharoptosis caused by oculomotor nerve paralysis with pseudo-Graefe phenomenon were collected in this study and the modified levator resection technique was applied to correct ptosis. The observation index after operation included the height of upper palpebral margin, the radian of double eyelid, the change of eye movement and the corresponding upper eyelid height, the eye closure function and the incidence of exposure keratitis. \u0000 \u0000 \u0000Results \u0000One case of oculomotor nerve palsy with moderate ptosis achieved an ideal height of upper eyelid after surgery, two cases of oculomotor nerve palsy with severe ptosis were undercorrection. All the three patients had smooth double eyelid radian, no obvious change of eye movement and corresponding upper eyelid height compared with preoperative. The two severe ptoses could close eyes well, but the moderate ptosis patient couldnot close eyes completely. \u0000 \u0000 \u0000Conclusions \u0000The technique of modified levator resection is an effective method to treat the moderate ptosis caused by oculomotor nerve paralysis with pseudo-Graefe phenomenon, but the results are unfavorable in the treatment of severe ptosis. \u0000 \u0000 \u0000Key words: \u0000Oculomotor nerve; Paralysis; Blepharoptosis; Pseudo-Graefe phenomenon, ptosis; Modified levator resection technique","PeriodicalId":10094,"journal":{"name":"Chinese Journal of Medical Aesthetics and Cosmetology","volume":"25 1","pages":"193-197"},"PeriodicalIF":0.0,"publicationDate":"2019-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42059247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Application of internal fixation technique with absorbable material for rhinoplasty to correct secondary nose deformity of unilateral cleft lip with or without cleft palate 可吸收材料内固定技术在单侧唇裂伴或不伴腭裂鼻整形术中的应用
Pub Date : 2019-04-15 DOI: 10.3760/CMA.J.ISSN.1671-0290.2019.02.014
Xia Zhou
Objective It is crucial to restore and fix the position of nasal septum which elaborates supportive function in rhinoplasty for secondary nose deformity of cleft lip and palate patients. The aim of this study was to explore the fixation technique with absorbable material in rhinoplasty for correction cleft lip nose deformity. Methods Nineteen cases with unilateral cleft lip /with or without palate cleft were included in this study. Absorbable screw was used to fix the implanted and re-positioned grafted cartilage septum which was shaped into strip with anterior nasal spine. Results Full success was achieved for all of 19 cases with internal fixation technique to re-position nasal septum to correct nasal deformity. There was no complications occurred. Conclusions Internal fixation technique with absorbable material could be used to correct secondary nasal deformity in cleft lip and palate patients and fixed re-positioned grafted cartilage septum can apply a strong support to ala nasi cartilage in cleft site. Key words: Cleft lip; Internal fixation; Nose; Deformity; Absorbable screw
目的探讨鼻中隔在唇腭裂继发性鼻畸形鼻成形术中的支撑作用,对鼻中隔的修复和固定至关重要。本研究旨在探讨可吸收材料在鼻整形术中的固定技术,以矫正唇裂鼻畸形。方法对19例单侧唇腭裂患者进行临床分析。采用可吸收螺钉固定已植入并重新定位的移植软骨间隔,该间隔与前鼻棘呈条状。结果采用鼻中隔复位内固定技术矫正鼻畸形19例,均获得圆满成功。没有出现并发症。结论可吸收材料内固定技术可用于唇腭裂患者继发性鼻畸形的矫正,固定复位移植软骨隔膜可对腭裂部位的鼻翼软骨提供强有力的支撑。关键词:唇裂;内固定;鼻子;变形;可吸收螺钉
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中华医学美学美容杂志
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