With global warming, crop productivity is increasingly threatened by heat stress. Source-sink relations—critical yet vulnerable components of plant productivity, especially under climate extremes—have recently become the focus of innovative biotechnological interventions. Lou et al. (2024) successfully used prime editing to engineer heat-resilient crops by enhancing carbon partitioning, thereby mitigating yield losses without compromising quality. This groundbreaking strategy offers a promising pathway for developing climate-smart crops and achieving sustainable agriculture, with broad implications for the future of food security in a warming world.
{"title":"Precision engineering of carbon partitioning enhances crop resilience to heat stress","authors":"Xusheng Zhao, Shengjie Chen, Zhili Wang, Hon-Ming Lam","doi":"10.1016/j.ncrops.2025.100086","DOIUrl":"10.1016/j.ncrops.2025.100086","url":null,"abstract":"<div><div>With global warming, crop productivity is increasingly threatened by heat stress. Source-sink relations—critical yet vulnerable components of plant productivity, especially under climate extremes—have recently become the focus of innovative biotechnological interventions. Lou et al. (2024) successfully used prime editing to engineer heat-resilient crops by enhancing carbon partitioning, thereby mitigating yield losses without compromising quality. This groundbreaking strategy offers a promising pathway for developing climate-smart crops and achieving sustainable agriculture, with broad implications for the future of food security in a warming world.</div></div>","PeriodicalId":100953,"journal":{"name":"New Crops","volume":"3 ","pages":"Article 100086"},"PeriodicalIF":0.0,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145265482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-15DOI: 10.1016/j.ncrops.2025.100084
Sujuan Xu , Hanhan Feng , Ting Li , Yinyi Zhang , Ze Wu , Genlou Sun , Nianjun Teng
WRKY genes represent a major family of transcription factors that play key roles in regulating plant responses to both biotic and abiotic stresses. While WRKY genes have been extensively studied in various plant species, their functions in lily remain largely unknown. Here, we identified 115 WRKY family genes in the lily genome through bioinformatics analyses. These genes encode proteins ranging from 72 to 708 amino acids, with predicted isoelectric points between 4.90 and 11.06. Phylogenetic analysis grouped these LdWRKY genes into clusters Ⅰ, Ⅱ, and Ⅲ, and promoter analysis identified numerous cis-acting elements related to growth, development, and hormone and stress responses. Gene expression heatmaps revealed significant variation in the expression of LdWRKY family members across different tissues, with 62 out of 115 LdWRKYs showing differential expression under heat stress conditions. Using transcriptome data, we cloned the heat stress-responsive candidate gene LdWRKY87, which encodes a nuclear-localized protein with transcriptional repression activity. Transient transformation assays confirmed the role of LdWRKY87 in regulating lily heat tolerance. Further studies suggest that LdWRKY87 may modulate heat tolerance by affecting the expression of heat tolerance-related genes, such as LdHSFA2, LdHSP70, and LdMBF1c. These findings provide a foundation for future studies on the regulatory mechanisms of WRKY transcription factors in lily heat tolerance.
{"title":"Genome-wide analysis of the WRKY gene family in lily and functional characterization of LdWRKY87 in response to heat stress","authors":"Sujuan Xu , Hanhan Feng , Ting Li , Yinyi Zhang , Ze Wu , Genlou Sun , Nianjun Teng","doi":"10.1016/j.ncrops.2025.100084","DOIUrl":"10.1016/j.ncrops.2025.100084","url":null,"abstract":"<div><div><em>WRKY</em> genes represent a major family of transcription factors that play key roles in regulating plant responses to both biotic and abiotic stresses. While <em>WRKY</em> genes have been extensively studied in various plant species, their functions in lily remain largely unknown. Here, we identified 115 <em>WRKY</em> family genes in the lily genome through bioinformatics analyses. These genes encode proteins ranging from 72 to 708 amino acids, with predicted isoelectric points between 4.90 and 11.06. Phylogenetic analysis grouped these <em>LdWRKY</em> genes into clusters Ⅰ, Ⅱ, and Ⅲ, and promoter analysis identified numerous <em>cis</em>-acting elements related to growth, development, and hormone and stress responses. Gene expression heatmaps revealed significant variation in the expression of <em>LdWRKY</em> family members across different tissues, with 62 out of 115 <em>LdWRKYs</em> showing differential expression under heat stress conditions. Using transcriptome data, we cloned the heat stress-responsive candidate gene <em>LdWRKY87</em>, which encodes a nuclear-localized protein with transcriptional repression activity. Transient transformation assays confirmed the role of LdWRKY87 in regulating lily heat tolerance. Further studies suggest that LdWRKY87 may modulate heat tolerance by affecting the expression of heat tolerance-related genes, such as <em>LdHSFA2</em>, <em>LdHSP70</em>, and <em>LdMBF1c</em>. These findings provide a foundation for future studies on the regulatory mechanisms of WRKY transcription factors in lily heat tolerance.</div></div>","PeriodicalId":100953,"journal":{"name":"New Crops","volume":"3 ","pages":"Article 100084"},"PeriodicalIF":0.0,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145362196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-25DOI: 10.1016/j.ncrops.2025.100083
XiaoFei Du, Yu Zhang, Hailong Guo
The Toll/interleukin-1 receptor (TIR) domain, a conserved immune signaling module across all kingdoms, is frequently fused to other domains to enhance host defense against microbial pathogens. TIR domains exhibit multifunctional enzymatic activities, catalyzing diverse substrates into structurally distinct nucleotide metabolites that serve as second messengers to couple TNL signaling to RNL activation. Two recent studies published in Science revealed that the EDS1–PAD4–ADR1 signaling module, which transduces TIR enzymatic activity into immune responses, is evolutionarily conserved in both monocots and dicots. Remarkably, this module is activated not only by plant TIR domains but also by bacterial TIR domains. Advancing our understanding of cross-kingdom TIR enzymatic functions and their downstream signaling mechanisms offers promising strategies for engineering durable disease resistance in crops.
{"title":"An evolutionarily conserved module links TIR signaling to disease resistance","authors":"XiaoFei Du, Yu Zhang, Hailong Guo","doi":"10.1016/j.ncrops.2025.100083","DOIUrl":"10.1016/j.ncrops.2025.100083","url":null,"abstract":"<div><div>The Toll/interleukin-1 receptor (TIR) domain, a conserved immune signaling module across all kingdoms, is frequently fused to other domains to enhance host defense against microbial pathogens. TIR domains exhibit multifunctional enzymatic activities, catalyzing diverse substrates into structurally distinct nucleotide metabolites that serve as second messengers to couple TNL signaling to RNL activation. Two recent studies published in <em>Science</em> revealed that the EDS1–PAD4–ADR1 signaling module, which transduces TIR enzymatic activity into immune responses, is evolutionarily conserved in both monocots and dicots. Remarkably, this module is activated not only by plant TIR domains but also by bacterial TIR domains. Advancing our understanding of cross-kingdom TIR enzymatic functions and their downstream signaling mechanisms offers promising strategies for engineering durable disease resistance in crops.</div></div>","PeriodicalId":100953,"journal":{"name":"New Crops","volume":"3 ","pages":"Article 100083"},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145104420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-10DOI: 10.1016/j.ncrops.2025.100082
Tongtong Xiao , Shidong Zhang , Yun Gu , Haiying Hu , Liangyong Sun , Chuwen Lu , Marilyn L. Warburton , Hui Li , Jiantang Zhu
Flavanone 3-hydroxylase (F3H) plays a pivotal role in the biosynthesis of flavonoid compounds, which are involved in growth and development processes, as well as stress responses in plants. However, little information has been uncovered about the F3H gene family in maize (Zea mays) to date. In this study, 15 ZmF3H genes were identified in the maize genome and clustered into four phylogenetic groups with homologs from other plant species. Expression profile analysis revealed that most ZmF3H genes exhibited differential expression patterns across various maize tissues. qRT-PCR expression analysis of ZmF3H genes under salt treatment identified ZmF3H6 as an excellent candidate gene for salt resistance. Overexpression of ZmF3H6 in Arabidopsis led to increased tolerance to salt stress, possibly by enhancing flavonol accumulation and antioxidant capacity. Conversely, a mutation in the sequence of ZmF3H6 resulted in compromised salt tolerance of maize seedlings. Molecular docking identified that ZmF3H6 binds to naringenin at specific amino acid residues necessary for hydroxylation. Dual-luciferase reporter and electrophoretic mobility shift assays demonstrated that the transcription factor ZmMYB33 activates ZmF3H6 expression by binding to the MBS domains in the ZmF3H6 promoter. Our findings provide a foundation for further investigation into the roles of the ZmF3H genes in plant abiotic stress responses and present a novel genetic resource for creating salt-resistant maize.
{"title":"Systematic analysis of the F3H family in maize reveals a role for ZmF3H6 in salt stress tolerance","authors":"Tongtong Xiao , Shidong Zhang , Yun Gu , Haiying Hu , Liangyong Sun , Chuwen Lu , Marilyn L. Warburton , Hui Li , Jiantang Zhu","doi":"10.1016/j.ncrops.2025.100082","DOIUrl":"10.1016/j.ncrops.2025.100082","url":null,"abstract":"<div><div>Flavanone 3-hydroxylase (F3H) plays a pivotal role in the biosynthesis of flavonoid compounds, which are involved in growth and development processes, as well as stress responses in plants. However, little information has been uncovered about the <em>F3H</em> gene family in maize (<em>Zea mays</em>) to date. In this study, 15 <em>ZmF3H</em> genes were identified in the maize genome and clustered into four phylogenetic groups with homologs from other plant species. Expression profile analysis revealed that most <em>ZmF3H</em> genes exhibited differential expression patterns across various maize tissues. qRT-PCR expression analysis of <em>ZmF3H</em> genes under salt treatment identified <em>ZmF3H6</em> as an excellent candidate gene for salt resistance. Overexpression of <em>ZmF3H6</em> in <em>Arabidopsis</em> led to increased tolerance to salt stress, possibly by enhancing flavonol accumulation and antioxidant capacity. Conversely, a mutation in the sequence of <em>ZmF3H6</em> resulted in compromised salt tolerance of maize seedlings. Molecular docking identified that ZmF3H6 binds to naringenin at specific amino acid residues necessary for hydroxylation. Dual-luciferase reporter and electrophoretic mobility shift assays demonstrated that the transcription factor ZmMYB33 activates <em>ZmF3H6</em> expression by binding to the MBS domains in the <em>ZmF3H6</em> promoter. Our findings provide a foundation for further investigation into the roles of the <em>ZmF3H</em> genes in plant abiotic stress responses and present a novel genetic resource for creating salt-resistant maize.</div></div>","PeriodicalId":100953,"journal":{"name":"New Crops","volume":"3 ","pages":"Article 100082"},"PeriodicalIF":0.0,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-05-19DOI: 10.1016/j.ncrops.2025.100081
Yilei Zhang , Panpan Li , Bin Yi , Dongli He
Plant mitochondria are vital organelles that play a decisive role in key agricultural traits, including cytoplasmic male sterility (CMS). CMS has been a cornerstone in the hybrid breeding of crops. Although many genes responsible for CMS in mitochondria have been identified, due to the lack of efficient plant mitochondrial transgenic technology and rapid mitochondrial isolation technology, the molecular mechanisms of their function and coordination with nuclear-encoded fertility recovery (Rf) genes are not fully understood. The double-layer membrane of plant mitochondria encases a relatively complex genome, posing challenges for direct genetic transformation and complicating the understanding of the CMS/Rf system. This review focuses on recent advances in the general characteristics, gene editing, and isolation techniques of plant mitochondria. We further explore the potential revolutionary applications of CMS by improving or creating new CMS or restoration lines based on the progress of plant mitochondria.
{"title":"Current advances in plant mitochondria: Application revolution of cytoplasmic male sterility","authors":"Yilei Zhang , Panpan Li , Bin Yi , Dongli He","doi":"10.1016/j.ncrops.2025.100081","DOIUrl":"10.1016/j.ncrops.2025.100081","url":null,"abstract":"<div><div>Plant mitochondria are vital organelles that play a decisive role in key agricultural traits, including cytoplasmic male sterility (CMS). CMS has been a cornerstone in the hybrid breeding of crops. Although many genes responsible for CMS in mitochondria have been identified, due to the lack of efficient plant mitochondrial transgenic technology and rapid mitochondrial isolation technology, the molecular mechanisms of their function and coordination with nuclear-encoded fertility recovery (<em>Rf</em>) genes are not fully understood. The double-layer membrane of plant mitochondria encases a relatively complex genome, posing challenges for direct genetic transformation and complicating the understanding of the CMS/Rf system. This review focuses on recent advances in the general characteristics, gene editing, and isolation techniques of plant mitochondria. We further explore the potential revolutionary applications of CMS by improving or creating new CMS or restoration lines based on the progress of plant mitochondria.</div></div>","PeriodicalId":100953,"journal":{"name":"New Crops","volume":"3 ","pages":"Article 100081"},"PeriodicalIF":0.0,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145265483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-05-02DOI: 10.1016/j.ncrops.2025.100080
Leidi Liu , Cheng Li , Feng Hu , Jingzhe Zhao , Roshan Zameer , Jingyao Li , Chengde Yu , Huazhong Shi , Zhifang Li , Chun-Peng Song , Changsong Zou
Cotton fiber length is a crucial attribute that significantly affects yarn production and fabric quality, making it a primary focus in cotton breeding efforts. Both current and previous studies have indicated a lack of correlation between fiber length and gene expression dynamics, underscoring the importance of phenotyping fiber elongation. Traditional methods for measuring fiber length, however, tend to be impractical and labor-intensive, particularly for developing fibers that are fragile and prone to twisting. In this study, we present an innovative phenotyping method to measure the elongation of developing cotton fibers. Our key findings reveal a strong linear relationship between the total volume of fiber bundles and fiber length. This relationship allows for the straightforward estimation of the linear correlation coefficient from the final fiber length and the final volume of the fiber bundle within a boll. Upon measuring both the fiber bundle volume and fiber length, we discovered that their growth dynamics were well-represented by a logistic curve. Additionally, the expression dynamics of several newly identified genes demonstrated a significant positive correlation with the rate of fiber elongation. This research marks an important advancement in quantifying gene expression dynamics and fiber elongation. We believe that measuring the elongation of developing fibers will greatly accelerate the development of high-quality cotton varieties and enhance our understanding of plant developmental biology.
{"title":"Enhancements in cotton fiber length phenotyping and optimization of weighted gene co-expression network analysis through fiber elongation rate metrics","authors":"Leidi Liu , Cheng Li , Feng Hu , Jingzhe Zhao , Roshan Zameer , Jingyao Li , Chengde Yu , Huazhong Shi , Zhifang Li , Chun-Peng Song , Changsong Zou","doi":"10.1016/j.ncrops.2025.100080","DOIUrl":"10.1016/j.ncrops.2025.100080","url":null,"abstract":"<div><div>Cotton fiber length is a crucial attribute that significantly affects yarn production and fabric quality, making it a primary focus in cotton breeding efforts. Both current and previous studies have indicated a lack of correlation between fiber length and gene expression dynamics, underscoring the importance of phenotyping fiber elongation. Traditional methods for measuring fiber length, however, tend to be impractical and labor-intensive, particularly for developing fibers that are fragile and prone to twisting. In this study, we present an innovative phenotyping method to measure the elongation of developing cotton fibers. Our key findings reveal a strong linear relationship between the total volume of fiber bundles and fiber length. This relationship allows for the straightforward estimation of the linear correlation coefficient from the final fiber length and the final volume of the fiber bundle within a boll. Upon measuring both the fiber bundle volume and fiber length, we discovered that their growth dynamics were well-represented by a logistic curve. Additionally, the expression dynamics of several newly identified genes demonstrated a significant positive correlation with the rate of fiber elongation. This research marks an important advancement in quantifying gene expression dynamics and fiber elongation. We believe that measuring the elongation of developing fibers will greatly accelerate the development of high-quality cotton varieties and enhance our understanding of plant developmental biology.</div></div>","PeriodicalId":100953,"journal":{"name":"New Crops","volume":"3 ","pages":"Article 100080"},"PeriodicalIF":0.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144570490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-11DOI: 10.1016/j.ncrops.2025.100079
Jiaxian He , Yifei Chen , Manting Zhang , Yongjian Qiu , Huapeng Zhou , Meina Li
Soil salinity is an increasing threat to global food security and environmental sustainability. Soybean, the leading dietary protein and oil content crop in animal feed, also provides humans with 30% of their dietary fat intake and contributes to 67% of global protein powder consumption annually. The improvement and utilization of saline-alkaline land can expand arable land for soybean production and decrease the yield penalty, ensuring food security for the growing world population. Over the past decades, identifying salt-tolerant cultivars and understanding salt stress signaling and responses in soybeans have made some progress. However, few successful studies about improved soybean field performance have been reported. Here, we provide recent advances in functionally characterized genes and major quantitative trait loci (QTLs) contributing to soybean salt tolerance. We focus on the strategies that we could take to achieve salt-tolerant soybean cultivars with high-yield, which includes unveiling the underlying cellular and molecular mechanisms that regulate the soybean response to high pH alkaline stress, to gain better knowledge of the soybean circadian clock and time-gate the response to saline-alkaline stress and minimize the fitness cost, and lay out the audacious plans to make soybean a halophyte. We aim to inspire the researchers in salt-tolerant breeding and research to new frontiers.
{"title":"Current perspectives on improving soybean performance on saline-alkaline lands","authors":"Jiaxian He , Yifei Chen , Manting Zhang , Yongjian Qiu , Huapeng Zhou , Meina Li","doi":"10.1016/j.ncrops.2025.100079","DOIUrl":"10.1016/j.ncrops.2025.100079","url":null,"abstract":"<div><div>Soil salinity is an increasing threat to global food security and environmental sustainability. Soybean, the leading dietary protein and oil content crop in animal feed, also provides humans with 30% of their dietary fat intake and contributes to 67% of global protein powder consumption annually. The improvement and utilization of saline-alkaline land can expand arable land for soybean production and decrease the yield penalty, ensuring food security for the growing world population. Over the past decades, identifying salt-tolerant cultivars and understanding salt stress signaling and responses in soybeans have made some progress. However, few successful studies about improved soybean field performance have been reported. Here, we provide recent advances in functionally characterized genes and major quantitative trait loci (QTLs) contributing to soybean salt tolerance. We focus on the strategies that we could take to achieve salt-tolerant soybean cultivars with high-yield, which includes unveiling the underlying cellular and molecular mechanisms that regulate the soybean response to high pH alkaline stress, to gain better knowledge of the soybean circadian clock and time-gate the response to saline-alkaline stress and minimize the fitness cost, and lay out the audacious plans to make soybean a halophyte. We aim to inspire the researchers in salt-tolerant breeding and research to new frontiers.</div></div>","PeriodicalId":100953,"journal":{"name":"New Crops","volume":"3 ","pages":"Article 100079"},"PeriodicalIF":0.0,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144622789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-05DOI: 10.1016/j.ncrops.2025.100078
Mingyang Hu , Chunxing Sun , Qun Feng , Lang Liu , Feng Yu , Tuo Zeng , Lei Gu , Hongcheng Wang , Xuye Du , Bowei Cai , Bin Zhu
Alien addition lines refer to the incorporation of one or more chromosomes from a donor species into the genomic background of a recipient species. These lines serve as crucial tools in genetic engineering, particularly for transferring beneficial genes and desirable traits from a donor genome to a recipient genome. In this study, we obtained three disomic alien addition lines (DAALs) of Brassica napus-Raphanus sativus (AACC + 2R, 2n = 40) from a complete set of monosomic alien addition lines (MAALs) through microspore culture techniques, utilizing PCR amplification with R chromosome-specific primers and fluorescence in situ hybridization (FISH). We conducted a comprehensive and systematic analysis of these DAALs and their parents. Phenotypically, these lines exhibit significantly distinct characteristics compared to their parental types. The silique length of line DR5 exceeds that of lines DR2 and DR4. The DAALs also display significantly reduced silique width and beak length compared to their parental lines. Regarding pollen viability, thousand seed weight, and seeds per pod, the DAALs demonstrate superior performance relative to the MAALs. Furthermore, the seeds from line DR4 are larger than those of the parental B. napus. Cytological observations indicate that the chromosome behavior of the DAALs is regular, with 20 bivalents encompassing 10 IIA-A, 9 IIC-C, and 1 IIR-R. At anaphase I, an even distribution of chromosomes to both sides of the pollen mother cells (PMCs) is observed in the DAALs. Overall, these DAALs are anticipated to have a substantial economic and agricultural impact on the enhancement of B. napus.
{"title":"Identification, characteristics, and cytological analysis of three disomic alien addition lines of Brassica napus- Raphanus sativus","authors":"Mingyang Hu , Chunxing Sun , Qun Feng , Lang Liu , Feng Yu , Tuo Zeng , Lei Gu , Hongcheng Wang , Xuye Du , Bowei Cai , Bin Zhu","doi":"10.1016/j.ncrops.2025.100078","DOIUrl":"10.1016/j.ncrops.2025.100078","url":null,"abstract":"<div><div>Alien addition lines refer to the incorporation of one or more chromosomes from a donor species into the genomic background of a recipient species. These lines serve as crucial tools in genetic engineering, particularly for transferring beneficial genes and desirable traits from a donor genome to a recipient genome. In this study, we obtained three disomic alien addition lines (DAALs) of <em>Brassica napus</em>-<em>Raphanus sativus</em> (AACC + 2R, 2n = 40) from a complete set of monosomic alien addition lines (MAALs) through microspore culture techniques, utilizing PCR amplification with R chromosome-specific primers and fluorescence <em>in situ</em> hybridization (FISH). We conducted a comprehensive and systematic analysis of these DAALs and their parents. Phenotypically, these lines exhibit significantly distinct characteristics compared to their parental types. The silique length of line DR5 exceeds that of lines DR2 and DR4. The DAALs also display significantly reduced silique width and beak length compared to their parental lines. Regarding pollen viability, thousand seed weight, and seeds per pod, the DAALs demonstrate superior performance relative to the MAALs. Furthermore, the seeds from line DR4 are larger than those of the parental <em>B. napus</em>. Cytological observations indicate that the chromosome behavior of the DAALs is regular, with 20 bivalents encompassing 10 II<sup>A-A</sup>, 9 II<sup>C-C</sup>, and 1 II<sup>R-R</sup>. At anaphase I, an even distribution of chromosomes to both sides of the pollen mother cells (PMCs) is observed in the DAALs. Overall, these DAALs are anticipated to have a substantial economic and agricultural impact on the enhancement of <em>B. napus.</em></div></div>","PeriodicalId":100953,"journal":{"name":"New Crops","volume":"3 ","pages":"Article 100078"},"PeriodicalIF":0.0,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144680004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-05DOI: 10.1016/j.ncrops.2025.100070
Mengyan Bai , Xinchen Hu , Wenxin Lin , Chunyan Peng , Huaqin Kuang , Xiangbin Zhong , Yinghua Li , Bo Chen , Jiyao Wang , Huarong Li , Baohui Liu , Fanjiang Kong , Yuefeng Guan
{"title":"Erratum to “Development of PmCDA1-based high-efficiency cytidine base editors (ChyCBEs) incorporating a GmRad51 DNA-binding domain in soybean” [New Crops 1 (2024) 100001]","authors":"Mengyan Bai , Xinchen Hu , Wenxin Lin , Chunyan Peng , Huaqin Kuang , Xiangbin Zhong , Yinghua Li , Bo Chen , Jiyao Wang , Huarong Li , Baohui Liu , Fanjiang Kong , Yuefeng Guan","doi":"10.1016/j.ncrops.2025.100070","DOIUrl":"10.1016/j.ncrops.2025.100070","url":null,"abstract":"","PeriodicalId":100953,"journal":{"name":"New Crops","volume":"2 ","pages":"Article 100070"},"PeriodicalIF":0.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143551388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-25DOI: 10.1016/j.ncrops.2025.100071
Yameng Liang, Feng Tian
Soybean is a facultative short-day plant, and its high photoperiod sensitivity poses challenges for breeding widely adapted soybean cultivars. Although the genetic basis of photoperiod in plants has been extensively studied, mutations in most of the photoperiod genes usually weaken sensitivity rather than eliminate it. Recently, Zhao et al. (2024) discovered that the E2 family plays a crucial role in determining soybean photoperiod sensitivity. The triple mutant e2 e2la e2lb exhibits similar flowering time under both long-day and short-day conditions. Further investigation uncovered a translational-transcriptional suppression loop between E2 and evening complex that constitutes another key factor in determining soybean photoperiod sensitivity.
{"title":"E2 family and evening complex identify soybean photoperiod sensitivity","authors":"Yameng Liang, Feng Tian","doi":"10.1016/j.ncrops.2025.100071","DOIUrl":"10.1016/j.ncrops.2025.100071","url":null,"abstract":"<div><div>Soybean is a facultative short-day plant, and its high photoperiod sensitivity poses challenges for breeding widely adapted soybean cultivars. Although the genetic basis of photoperiod in plants has been extensively studied, mutations in most of the photoperiod genes usually weaken sensitivity rather than eliminate it. Recently, Zhao et al. (2024) discovered that the E2 family plays a crucial role in determining soybean photoperiod sensitivity. The triple mutant <em>e2 e2la e2lb</em> exhibits similar flowering time under both long-day and short-day conditions. Further investigation uncovered a translational-transcriptional suppression loop between E2 and evening complex that constitutes another key factor in determining soybean photoperiod sensitivity.</div></div>","PeriodicalId":100953,"journal":{"name":"New Crops","volume":"2 ","pages":"Article 100071"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143610690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号