Pub Date : 2018-04-01DOI: 10.1016/j.labcli.2017.09.004
Raquel Ferreirós-Martínez , Beatriz Hidalgo Calero , María Carmen Lorenzo Lozano , Rosa Carbonell Muñoz , María Concepción Alonso-Cerezo
{"title":"Herramientas para la evaluación de las competencias profesionales","authors":"Raquel Ferreirós-Martínez , Beatriz Hidalgo Calero , María Carmen Lorenzo Lozano , Rosa Carbonell Muñoz , María Concepción Alonso-Cerezo","doi":"10.1016/j.labcli.2017.09.004","DOIUrl":"10.1016/j.labcli.2017.09.004","url":null,"abstract":"","PeriodicalId":101105,"journal":{"name":"Revista del Laboratorio Clínico","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.labcli.2017.09.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84226384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-01DOI: 10.1016/j.labcli.2017.11.003
Valentín Moreno Carbonell , Esther Hernández Frutos , María José Bartolomé Albístegui , Israel Arribas Montero , Florentino Barbadillo Izquierdo , María Luisa Bartolomé Cano
Kabuki syndrome (KS) is a rare genetic disease that usually involves significant cardiac and immunological disorders. Most patients are diagnosed in the first years of life, despite the fact that the age of onset is not well-defined. Affected patients have recurrent infections due to their immunosuppression, and may reach a state of agranulocytosis. Although their prognosis is quite favourable, their life expectancy is determined by these types of complications. Therefore, to understand its particular analytical characteristics is interesting from the point of view of the Haematology Laboratory to contribute to their diagnosis and follow-up. The case is presented of a patient diagnosed with KS.
{"title":"Agranulocitosis en niña de 6 años. ¿Qué hay detrás?","authors":"Valentín Moreno Carbonell , Esther Hernández Frutos , María José Bartolomé Albístegui , Israel Arribas Montero , Florentino Barbadillo Izquierdo , María Luisa Bartolomé Cano","doi":"10.1016/j.labcli.2017.11.003","DOIUrl":"10.1016/j.labcli.2017.11.003","url":null,"abstract":"<div><p>Kabuki syndrome (KS) is a rare genetic disease that usually involves significant cardiac and immunological disorders. Most patients are diagnosed in the first years of life, despite the fact that the age of onset is not well-defined. Affected patients have recurrent infections due to their immunosuppression, and may reach a state of agranulocytosis. Although their prognosis is quite favourable, their life expectancy is determined by these types of complications. Therefore, to understand its particular analytical characteristics is interesting from the point of view of the Haematology Laboratory to contribute to their diagnosis and follow-up. The case is presented of a patient diagnosed with KS.</p></div>","PeriodicalId":101105,"journal":{"name":"Revista del Laboratorio Clínico","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.labcli.2017.11.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78026765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-01DOI: 10.1016/j.labcli.2017.05.003
F. Javier Gella Tomás, M. Jesús Andrés Otero, Raül Rigo Bonnin, Francesca Canalias Reverter, Ruth Cano Corres, Sara Esteve Poblador, Bernardino González de la Presa, Rosa López Martínez, Inmaculada Pérez de Algaba
{"title":"Nomenclatura y unidades de las propiedades biológicas. Recomendación (2016)","authors":"F. Javier Gella Tomás, M. Jesús Andrés Otero, Raül Rigo Bonnin, Francesca Canalias Reverter, Ruth Cano Corres, Sara Esteve Poblador, Bernardino González de la Presa, Rosa López Martínez, Inmaculada Pérez de Algaba","doi":"10.1016/j.labcli.2017.05.003","DOIUrl":"10.1016/j.labcli.2017.05.003","url":null,"abstract":"","PeriodicalId":101105,"journal":{"name":"Revista del Laboratorio Clínico","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.labcli.2017.05.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89334805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-01DOI: 10.1016/j.labcli.2017.09.006
Alexandre Xabier Obelleiro Campos , Elena Zafón Chuliá
{"title":"Importancia de los niveles de vitamina D en el manejo del asma","authors":"Alexandre Xabier Obelleiro Campos , Elena Zafón Chuliá","doi":"10.1016/j.labcli.2017.09.006","DOIUrl":"https://doi.org/10.1016/j.labcli.2017.09.006","url":null,"abstract":"","PeriodicalId":101105,"journal":{"name":"Revista del Laboratorio Clínico","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.labcli.2017.09.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91757825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-01DOI: 10.1016/j.labcli.2017.11.007
Laura Correa Martínez , Cristina Eugenia Gaona Álvarez , María Pierna Álvarez , Raquel de la Fuente del Río , Carmen González Velasco , Beatriz Sacristán Enciso
Enterococcal meningitis is a very rare clinical entity, accounting for 0.3-4% of bacterial meningitis, with Enterococcus faecalis (E. faecalis) being the most common isolated species. There are risk factors for its development in newborns, such as maternal intrapartum fever, chorioamnionitis, prematurity, or previous intestinal resection.
The aim of this article is to describe a case of meningitis associated with early sepsis caused by E. faecalis in a newborn with fever, irritability, and in a poor general condition at 24 hours of birth, coinciding with normal cytochemical parameters in cerebrospinal fluid.
The clinical and analytical findings suggested an infectious process, which was confirmed by the isolation of E. faecalis in cerebrospinal fluid. The patient outcome was favourable after treatment with ampicillin and gentamicin. The epidemiological history suggested a vertical transmission.
{"title":"Meningitis neonatal por Enterococcus faecalis","authors":"Laura Correa Martínez , Cristina Eugenia Gaona Álvarez , María Pierna Álvarez , Raquel de la Fuente del Río , Carmen González Velasco , Beatriz Sacristán Enciso","doi":"10.1016/j.labcli.2017.11.007","DOIUrl":"10.1016/j.labcli.2017.11.007","url":null,"abstract":"<div><p>Enterococcal meningitis is a very rare clinical entity, accounting for 0.3-4% of bacterial meningitis, with <em>Enterococcus faecalis (E. faecalis)</em> being the most common isolated species. There are risk factors for its development in newborns, such as maternal intrapartum fever, chorioamnionitis, prematurity, or previous intestinal resection.</p><p>The aim of this article is to describe a case of meningitis associated with early sepsis caused by <em>E. faecalis</em> in a newborn with fever, irritability, and in a poor general condition at 24<!--> <!-->hours of birth, coinciding with normal cytochemical parameters in cerebrospinal fluid.</p><p>The clinical and analytical findings suggested an infectious process, which was confirmed by the isolation of <em>E. faecalis</em> in cerebrospinal fluid. The patient outcome was favourable after treatment with ampicillin and gentamicin. The epidemiological history suggested a vertical transmission.</p></div>","PeriodicalId":101105,"journal":{"name":"Revista del Laboratorio Clínico","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.labcli.2017.11.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73604156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-01DOI: 10.1016/j.labcli.2017.06.002
Alicia Beteta López , Lorena Vega Prado , José Martínez Alarcón , Francisco Alba García , Adolfo Blanco Jarava , Alberto Galiana Ivars
Streptococcus suis is an important pathogen associated with a wide range of diseases in pigs, constituting one of the most important problems of the swine industry around the world.
However, infection with S. suis in humans is very rare and is considered an unusual zoonosis because from 1968 until 2004 had described only around 250 cases around the world.
The emergence of some very large outbreaks of infection with S. suis in Europe and Southeast Asia from the year 2005, and the increase in the incidence of cases in other European countries, has made to think that we can find about a process of emergence of these infections.
S. suis is a Gram-positive facultative anaerobic coccus, belonging to R, S, and T Lancefield groups. Until recently, there have been major difficulties in the identification and differentiation of this organism of other Streptococcus species, what could be the cause of an underestimation of these infections. It's currently with diagnostic tools and molecular typing that allow a better characterization of the isolates.
The most frequent S. suis in humans infection is meningitis, but endocarditis, pneumonia, cellulitis, rhabdomyolysis, arthritis, spondylodiscitis, abscess epidural, peritonitis, enteritis, endophthalmitis, uveitis, septicemia with septic shock and multiorgan failure streptococcal toxic shock syndrome have also been reported.
In our country less than 20 cases of S. suis infection have been documented, so we believe interest communicate a new case of S. suis meningitis.
{"title":"Meningitis por Streptococcus suis: ¿una zoonosis emergente?","authors":"Alicia Beteta López , Lorena Vega Prado , José Martínez Alarcón , Francisco Alba García , Adolfo Blanco Jarava , Alberto Galiana Ivars","doi":"10.1016/j.labcli.2017.06.002","DOIUrl":"https://doi.org/10.1016/j.labcli.2017.06.002","url":null,"abstract":"<div><p><em>Streptococcus suis</em> is an important pathogen associated with a wide range of diseases in pigs, constituting one of the most important problems of the swine industry around the world.</p><p>However, infection with <em>S.<!--> <!-->suis</em> in humans is very rare and is considered an unusual zoonosis because from 1968 until 2004 had described only around 250 cases around the world.</p><p>The emergence of some very large outbreaks of infection with <em>S.<!--> <!-->suis</em> in Europe and Southeast Asia from the year 2005, and the increase in the incidence of cases in other European countries, has made to think that we can find about a process of emergence of these infections.</p><p><em>S.<!--> <!-->suis</em> is a Gram-positive facultative anaerobic coccus, belonging to R, S, and T Lancefield groups. Until recently, there have been major difficulties in the identification and differentiation of this organism of other <em>Streptococcus</em> species, what could be the cause of an underestimation of these infections. It's currently with diagnostic tools and molecular typing that allow a better characterization of the isolates.</p><p>The most frequent <em>S.<!--> <!-->suis</em> in humans infection is meningitis, but endocarditis, pneumonia, cellulitis, rhabdomyolysis, arthritis, spondylodiscitis, abscess epidural, peritonitis, enteritis, endophthalmitis, uveitis, septicemia with septic shock and multiorgan failure streptococcal toxic shock syndrome have also been reported.</p><p>In our country less than 20 cases of <em>S.<!--> <!-->suis</em> infection have been documented, so we believe interest communicate a new case of <em>S.<!--> <!-->suis</em> meningitis.</p></div>","PeriodicalId":101105,"journal":{"name":"Revista del Laboratorio Clínico","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.labcli.2017.06.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90125588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Assessment of the quality of the 24-hour urine sample (urine-24 h) is a challenge in the clinical laboratory. Different strategies based on urinary creatinine excretion have been proposed for the detection of poorly collected samples. The objective of this study was to evaluate the performance of two of these strategies: one based on the reference interval of urinary creatinine excretion (IR-Strategy), and the other one proposed by the Swiss Survey on Salt Group (S-Strategy).
Materials and methods
The study was carried out in the year 2016 with a population from Argentina. A total of 69 adult subjects were included in the study. Complete urine samples (N = 69) and incomplete samples (N = 69) were obtained. These were classified as poor, and well collected, according to the two strategies evaluated. Kappa coefficient, proportion of incorrectly classified samples, and positive and negative likelihood ratios (LR) were calculated. The difference was considered statistically significant for a P < .05. The study was approved by a local Bioethics Committee, and the informed consent was obtained from each participant.
Results
Strategy-IR vs. Strategy-S: Kappa coefficient: 0.26 (95% CI; 0.10-0.42; P = .020) vs. 0.51 (95% CI; 0.37-0.64; P = .000); incorrectly classified samples: 37% vs. 25% (P = .000); positive LR: 1.7 (95% CI; 1.2-2.4) vs. 9.8 (95% CI; 3.7-25.8); negative LR: 0.6 (95% CI; 0.4-0.8) vs. 0.5 (95% CI; 0.4-0.6).
Conclusion
The S-Strategy showed a better performance than the IR-Strategy in the detection of poorly collected urine samples.
24小时尿液样本(尿-24小时)的质量评估是临床实验室面临的一个挑战。基于尿肌酐排泄的不同策略已被提出用于检测收集不良的样本。本研究的目的是评估其中两种策略的效果:一种基于尿肌酐排泄参考区间(IR-Strategy),另一种由瑞士盐组调查(S-Strategy)提出。材料和方法该研究于2016年进行,研究对象来自阿根廷。共有69名成人受试者参与了这项研究。取完整尿样(N = 69)和不完整尿样(N = 69)。根据所评估的两种战略,它们被归类为较差和收集得很好。计算Kappa系数、误分类样本比例、正负似然比(LR)。P <. 05。本研究经当地生物伦理委员会批准,并获得每位参与者的知情同意。结果strategy - ir vs. Strategy-S: Kappa系数:0.26 (95% CI;0.10 - -0.42;P = 0.020) vs. 0.51 (95% CI;0.37 - -0.64;p = .000);错误分类的样本:37% vs. 25% (P = .000);阳性LR: 1.7 (95% CI;1.2-2.4) vs. 9.8 (95% CI;3.7 - -25.8);负LR: 0.6 (95% CI;0.4-0.8) vs. 0.5 (95% CI;0.4 - -0.6)。结论s策略比ir策略对收集不良尿样的检测效果更好。
{"title":"Calidad en la etapa preanalítica: evaluación de dos estrategias para verificar la correcta recogida de orina de 24 horas","authors":"Graciela Laura Pennacchiotti , Gisela Unger , Silvia Fabiana Benozzi , Amparo Campion","doi":"10.1016/j.labcli.2017.11.001","DOIUrl":"10.1016/j.labcli.2017.11.001","url":null,"abstract":"<div><h3>Introduction</h3><p>Assessment of the quality of the 24-hour urine sample (urine-24<!--> <!-->h) is a challenge in the clinical laboratory. Different strategies based on urinary creatinine excretion have been proposed for the detection of poorly collected samples. The objective of this study was to evaluate the performance of two of these strategies: one based on the reference interval of urinary creatinine excretion (IR-Strategy), and the other one proposed by the Swiss Survey on Salt Group (S-Strategy).</p></div><div><h3>Materials and methods</h3><p>The study was carried out in the year 2016 with a population from Argentina. A total of 69 adult subjects were included in the study. Complete urine samples (N<!--> <!-->=<!--> <!-->69) and incomplete samples (N<!--> <!-->=<!--> <!-->69) were obtained. These were classified as poor, and well collected, according to the two strategies evaluated. Kappa coefficient, proportion of incorrectly classified samples, and positive and negative likelihood ratios (LR) were calculated. The difference was considered statistically significant for a <em>P</em> <!--><<!--> <!-->.05. The study was approved by a local Bioethics Committee, and the informed consent was obtained from each participant.</p></div><div><h3>Results</h3><p>Strategy-IR vs. Strategy-S: Kappa coefficient: 0.26 (95% CI; 0.10-0.42; <em>P</em> <!-->=<!--> <!-->.020) vs. 0.51 (95% CI; 0.37-0.64; <em>P</em> <!-->=<!--> <!-->.000); incorrectly classified samples: 37% vs. 25% (<em>P</em> <!-->=<!--> <!-->.000); positive LR: 1.7 (95% CI; 1.2-2.4) vs. 9.8 (95% CI; 3.7-25.8); negative LR: 0.6 (95% CI; 0.4-0.8) vs. 0.5 (95% CI; 0.4-0.6).</p></div><div><h3>Conclusion</h3><p>The S-Strategy showed a better performance than the IR-Strategy in the detection of poorly collected urine samples.</p></div>","PeriodicalId":101105,"journal":{"name":"Revista del Laboratorio Clínico","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.labcli.2017.11.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74271300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.1016/j.labcli.2017.09.002
Marta Lucas Javato, Inmaculada Domínguez Pascual, Ana Isabel Álvarez Ríos, Manuel Conde Sánchez, Juan Miguel Guerrero Montavez
Thyroid diseases, after diabetes mellitus, are among the most common endocrine disorders during pregnancy, with an incidence of 5-10%. Early detection and treatment is important, as they can have negative consequences for both the mother and the foetus.
Hyperthyroidism is less frequent than hypothyroidism during pregnancy, being between 0.1% and 1%. It is characterised by a low thyrotropin with elevated thyroid hormones, with Graves’ disease being the most frequent cause (85% of cases).
The following is the case of an infant with primary hyperthyroidism of autoimmune origin, the son of a mother without previous diagnosis of hyperthyroidism during gestation.
{"title":"Gestante eutiroidea con anticuerpos estimulantes del receptor de tirotropina positivos: manifestación tardía de hipertiroidismo neonatal. A propósito de un caso","authors":"Marta Lucas Javato, Inmaculada Domínguez Pascual, Ana Isabel Álvarez Ríos, Manuel Conde Sánchez, Juan Miguel Guerrero Montavez","doi":"10.1016/j.labcli.2017.09.002","DOIUrl":"10.1016/j.labcli.2017.09.002","url":null,"abstract":"<div><p>Thyroid diseases, after diabetes mellitus, are among the most common endocrine disorders during pregnancy, with an incidence of 5-10%. Early detection and treatment is important, as they can have negative consequences for both the mother and the foetus.</p><p>Hyperthyroidism is less frequent than hypothyroidism during pregnancy, being between 0.1% and 1%. It is characterised by a low thyrotropin with elevated thyroid hormones, with Graves’ disease being the most frequent cause (85% of cases).</p><p>The following is the case of an infant with primary hyperthyroidism of autoimmune origin, the son of a mother without previous diagnosis of hyperthyroidism during gestation.</p></div>","PeriodicalId":101105,"journal":{"name":"Revista del Laboratorio Clínico","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.labcli.2017.09.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82398808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.1016/j.labcli.2017.09.001
María del Carmen Pasquel
{"title":"La acreditación en Latinoamérica con la norma 15189 para los laboratorios clínicos","authors":"María del Carmen Pasquel","doi":"10.1016/j.labcli.2017.09.001","DOIUrl":"10.1016/j.labcli.2017.09.001","url":null,"abstract":"","PeriodicalId":101105,"journal":{"name":"Revista del Laboratorio Clínico","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.labcli.2017.09.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76560133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}