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Results with the group 4 antibodies 结果与第4组抗体
Revue Fran?aise de Transfusion et Immuno-hématologie
Pub Date : 1988-04-01 DOI: 10.1016/S0338-4535(88)80120-X
A.E.G. Kr. Von dem Borne
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引用次数: 0
Test results of four monoclonal antibodies against markers of the Lutheran system 四种针对路德系统标记物的单克隆抗体的检测结果
Revue Fran?aise de Transfusion et Immuno-hématologie
Pub Date : 1988-04-01 DOI: 10.1016/S0338-4535(88)80139-9
Y. Okubo, T. Seno, H. Yamano, H. Yamaguchi

We would like to report on the test results of the four monoclonal antibodies distributed by Dr. Rouger.

These antibodies were tested by the use of the red cells of the dominant type In (Lu) and recessive type Lu (a - b -), some of them were from donors of Japanese Red Cross Blood Centers and SCARF's sample.

我们想报告Rouger博士分发的四种单克隆抗体的检测结果。这些抗体采用显性型In (Lu)和隐性型Lu (a - b -)红细胞检测，部分来自日本红十字会血液中心的献血者和SCARF的样本。
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引用次数: 0
Immunological characterization of 21 human anti-Rh (D) monoclonal antibodies 21种人抗rh (D)单克隆抗体的免疫学特性
Revue Fran?aise de Transfusion et Immuno-hématologie
Pub Date : 1988-04-01 DOI: 10.1016/S0338-4535(88)80095-3
P.Y. Le Pennec, M.T. Klein, M. Le Besnerais, G. Tsikas, F. Champomier, D. Goossens, Ph. Rouger

In this work we studied 21 human monoclonal antibodies (MABs) which appeared during the screening tests to have an anti-Rh (D) specificity. These antibodies were then tested especially by agglutination techniques using a panel of human red blood cells RBC of common and rare phenotypes in order to:

  • 1 -

    Confirm their specificity

  • 2 -

    Investigate their reactivity with Rh variants

  • 3 -

    Determine their in vitro potency (titre and quantitation) using various techniques.

在这项工作中，我们研究了筛选试验中出现的21种具有抗rh (D)特异性的人单克隆抗体(mab)。然后使用一组常见和罕见表型的人红细胞(RBC)对这些抗体进行凝集技术测试，以便:1 -确认其特异性2 -研究其与Rh变异的反应性3 -使用各种技术确定其体外效价(滴度和定量)。
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引用次数: 3
Co-ordinator's report on group 4: Antibodies to sialoglycoproteins (glycophorins) 第4组:唾液糖蛋白抗体(糖蛋白)协调人报告
Revue Fran?aise de Transfusion et Immuno-hématologie
Pub Date : 1988-04-01 DOI: 10.1016/S0338-4535(88)80122-3
D.J. Anstee (Co-ordinator)
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引用次数: 6
Monoclonal antibodies against band 3 protein 抗带3蛋白单克隆抗体
Revue Fran?aise de Transfusion et Immuno-hématologie
Pub Date : 1988-04-01 DOI: 10.1016/S0338-4535(88)80140-5
E. Lisowska, M. Czerwiński
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引用次数: 0
Evaluation of monoclonal anti-Rh antibodies as reagents for blood grouping and for the identification of red cell membrane components associated with Rh antigen activity 评价单克隆抗Rh抗体作为血型鉴定试剂和与Rh抗原活性相关的红细胞膜成分
Revue Fran?aise de Transfusion et Immuno-hématologie
Pub Date : 1988-04-01 DOI: 10.1016/S0338-4535(88)80098-9
S. Moore, C. Green, A. McGowan

Monoclonal anti-D reagents should ideally be able to detect cells which have deletions of the D « antigen mosaic , for example categories DV and DVI which are not infrequently found in the donor population. We investigated the ability of the monoclonal anti-D reagents to detect these cells and in addition DVII and DB cells. Monoclonal antibodies of other stated specificities were evaluated for specificity. All the monoclonal antibodies were evaluated as reagents for the investigation of red cell membrane structure using the immune complex isolation procedure of Moore et al. [1].

理想情况下，单克隆抗-D试剂应该能够检测到D«抗原镶嵌缺失的细胞，例如在供体人群中并不罕见的DV和DVI类。我们研究了单克隆抗d试剂对这些细胞的检测能力，以及对dvi和DB细胞的检测能力。评估其他特异性单克隆抗体的特异性。采用Moore等人的免疫复合体分离方法对所有单克隆抗体进行评价，作为研究红细胞膜结构的试剂。
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引用次数: 1
Evaluation of human and murine monoclonal anti-rhésus antibodies 人源和鼠源单克隆抗鼠毛霉抗体的鉴定
Revue Fran?aise de Transfusion et Immuno-hématologie
Pub Date : 1988-04-01 DOI: 10.1016/S0338-4535(88)80103-X
L. Mannessier , H. Broly

Thirty monoclonal anti-Rhesus antibodies were tested : 21 anti-D, 4 anti-E, 4 anti-e and 1 anti-c, ragarding their specificity, avidity, intensity, titer and score. Their type, mode and mean were determined by flow cytometry assay.

Then, we tried to determine if the anti-D could adequately dtect Du samples and also studied their behaviour with 13 partial and 2 depleted D antigens.

检测30种单克隆抗恒河猴抗体:抗d抗体21种，抗e抗体4种，抗e抗体4种，抗c抗体1种，对其特异性、亲和力、强度、滴度和评分进行比较。流式细胞术测定其类型、模式及平均值。然后，我们试图确定anti-D是否能够充分检测Du样品，并研究了它们与13种部分和2种耗尽的D抗原的行为。
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引用次数: 1
Co-ordinator's report on group 3 : Monoclonal Rh antibodies : serological and biological studies 协调人报告第3组:单克隆Rh抗体:血清学和生物学研究
Revue Fran?aise de Transfusion et Immuno-hématologie
Pub Date : 1988-04-01 DOI: 10.1016/S0338-4535(88)80111-9
P. Tippett

Thirty-nine monoclonal antibodies (MAbs), of human or of murine origin, were tested by 18 laboratories (Table I). Several techniques were used to define the characteristics and in the quantitative determinations of these antibodies. Serological tests evaluated their specificity and activity and their ability to detect variant red cell antigens. Biological assays were performed in an attempt to determine their potential therapeutic usefulness.

18个实验室对39种人源或鼠源单克隆抗体(mab)进行了测试(表1)。使用了几种技术来确定这些抗体的特征和定量测定。血清学试验评估了它们的特异性和活性，以及它们检测变异红细胞抗原的能力。进行了生物测定，试图确定其潜在的治疗作用。
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引用次数: 7
Observations on 5 monoclonal antibody samples directed against antigens in the Kell blood group system 针对Kell血型系统抗原的5种单克隆抗体的观察
Revue Fran?aise de Transfusion et Immuno-hématologie
Pub Date : 1988-04-01 DOI: 10.1016/S0338-4535(88)80127-2
T. Zelinski, H. Kaita, G. Coghlan, M. Lewis
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引用次数: 0
Groupe 3-anti Rh 第3组抗Rh
Revue Fran?aise de Transfusion et Immuno-hématologie
Pub Date : 1988-04-01 DOI: 10.1016/S0338-4535(88)80097-7
J. Dante-Paire, F. Martel, C. Blourde, P. Debeaux, D. Rigal
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引用次数: 0
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