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Detection of nutritional stress in sugarcane by VIS-NIR-SWIR reflectance spectroscopy VIS-NIR-SWIR反射光谱法检测甘蔗营养胁迫
IF 0.8 4区 农林科学 Q3 AGRONOMY Pub Date : 2023-06-16 DOI: 10.1590/0103-8478cr20220543
Carlos Augusto Alves Cardoso Silva, P. Fiorio, R. Rizzo, R. Rossetto, A. Vitti, F. Dias, K. A. Oliveira, Michaela Bárbara Neto
ABSTRACT: This study applied spectroradiometry techniques with hyperspectral data to identify the correlations between sugarcane leaf reflectance and the contents of Nitrogen (N), phosphorus (P), Potassium (K), Sulfur (S), Calcium (Ca) and Magnesium (Mg). During the harvests 2019/20 and 2020/21, sugarcane was introduced to nutritional stress by the application of limestone doses. Liming was applied in a fractional way and, at the end of five years, the amounts corresponded to 0, 9, 15 and 21 t ha-1 of dolomitic limestone. The leaf hyperspectral reflectance data and the state of nutrients in the exponential growth phase of the culture were registered. The wavelengths correlated with N, P, K, S, Ca and Mg were identified using the Spearman’s correlation analysis. The test of similarity (ANOSIM) and the Principal Component Analysis (PCA) were applied to evaluate data variability, as well as the Partial Least Squares Regression (PLSR) for the prediction of the nutritional contents. The order of the degree of correlation in the region of visible was: P > K > N > Ca > S > Mg and for the region of the near infrared: P > K > Ca > N > S > Mg. P presented peaks with high correlations in the wavelengths 706-717 nm (-0.78) and 522-543 nm (-0.76). The values of the PLSR registered the best spectral responses in the region of VIS and red-edge, regions that are more sensitive to the deficiency of sulfur, potassium and phosphorus.
摘要:本研究利用高光谱数据,利用光谱辐射测定技术,研究了甘蔗叶片反射率与氮(N)、磷(P)、钾(K)、硫(S)、钙(Ca)和镁(Mg)含量的关系。在2019/20和2020/21收获期间,甘蔗通过施用石灰石剂量而受到营养胁迫。石灰是分次施用的,在5年结束时,石灰的用量分别为0、9、15和21吨/公顷。记录了指数生长期叶片高光谱反射率数据和营养物质状况。利用Spearman相关分析确定了与N、P、K、S、Ca和Mg相关的波长。采用相似性检验(ANOSIM)和主成分分析(PCA)评估数据的可变性,并采用偏最小二乘回归(PLSR)预测营养含量。在可见光区域的相关度顺序为:P > K > N > Ca > S > Mg,在近红外区域的相关度顺序为:P > K > Ca > N > S > Mg。P值在706 ~ 717 nm(-0.78)和522 ~ 543 nm(-0.76)处呈高相关性。在对缺硫、缺钾、缺磷较为敏感的VIS区和红边区,PLSR值的光谱响应最好。
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引用次数: 1
Growth and light environment of fruit trees in silvipastoral systems for rearing of dairy herds 林牧系统中果树生长和光环境的研究
IF 0.8 4区 农林科学 Q3 AGRONOMY Pub Date : 2023-06-02 DOI: 10.1590/0103-8478cr20220017
C. Giustina, C. A. Carvalho, R. Carnevalli, M. Romano, Francisco das Chagas de Abreu Pereira
ABSTRACT: Silvopastoral systems can have animal welfare and sustainability benefits because trees continually remove carbon from atmosphere, reducing greenhouse effects. Thisstudy identified the most promising fruit trees for inclusion in silvopastoral systems to dairy cattle calves. This experiment was conducted at EmbrapaAgrossilvipastoril, Brazil, between 2014 and 2018. Five silvopastoral systems with fruit trees and ‘Tifton-85’ grass were designed to evaluate tree growth and light environment under the canopies. Data were analyzed using SAS® and PDIFF (P < 0.10). Caja fruit trees had the greatest tree height (5.4 m) and trunk diameter (23.4 cm), while acerola fruit tree had the smallest (1.8 m and 8.3 cm, respectively). At 42 months (drought 2017), caja, cashew, and guava trees had similar heights. Guava trees had the highest light interception (89.3%), both cashew cultivars provided medium levels of shade (50 to 60% LI) and with greater constancy between the rainy and dry seasons. The systems that showed increased light interception during the drought period were those with CCP76 in 2017 and EMB51 in 2018. Higher incidences of wavelengths of the spectral composition of light occurred between the rainfall (2015) and drought (2017) periods, and greater differences in the ratio of red:far red in 2015. By 2018, there were no more differences between the rainy and dry seasons for the spectral composition of light under the tree canopies. Cashews and guava trees have adequate growth and light environment to support silvopastoral systems but Caja and acerola fruit trees showed limitations.
摘要:森林生态系统具有动物福利和可持续发展效益,因为树木不断地从大气中吸收碳,减少温室效应。本研究确定了最有前途的果树纳入到奶牛犊牛的银寄生系统中。该实验于2014年至2018年在巴西的EmbrapaAgrossilvipastoril进行。设计了5个有果树和“蒂夫顿-85”草的银栖系统,以评估树冠下树木的生长和光环境。采用SAS®和PDIFF分析数据(P < 0.10)。树高最高的是Caja果树(5.4 m),树干直径23.4 cm,最小的是针叶果树(1.8 m),树干直径8.3 cm。在第42个月(2017年干旱),caja,腰果和番石榴树的高度相似。番石榴树截光率最高(89.3%),两种腰果品种提供中等水平的遮荫(50%至60% LI),雨季和旱季之间具有更大的稳定性。在干旱期间,光拦截增加的系统是2017年的CCP76和2018年的EMB51。在降雨(2015年)和干旱(2017年)期间,光的光谱组成波长的发生率更高,2015年红远红比的差异更大。到2018年,树冠下光光谱组成在雨季和旱季之间不再存在差异。腰果和番石榴有足够的生长和光照环境来支持森林系统,但Caja和针叶果树存在局限性。
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引用次数: 0
Droplet spectrum characteristics and drift potential of different droplet classes and spray volumes application of atrazine with nicosulfuron 氟磺隆与阿特拉津在不同雾滴类别和喷雾量下的光谱特性及漂移势
IF 0.8 4区 农林科学 Q3 AGRONOMY Pub Date : 2023-05-26 DOI: 10.1590/0103-8478cr20220509
Arthur Duarte Rodrigues Neto, Naiane Antunes Alves Ribeiro, Fagner Angelo da Silva e Oliveira, G. A. Faria, E. P. Prado
ABSTRACT: In this research, the Volumetric Median Diameters (VMDs), Relative Span (RS), percentage of droplets with a diameter smaller than 100 µm (V100), and percentage ofdrift (% drift) of four droplet classes (Fine, Medium, Coarse, and Very Coarse) were analyzedin two spray volumes (77 L.ha-1and 144 L.ha-1) employinga tank-mix of atrazine and nicosulfuron herbicides (2500 + 15 gaiha-1), usingParticle/Droplet Imaging Analyze (PDIA) and wind tunnel techniques. The experiments were performed under Completely Randomized Design, in a 4 × 2 factorial scheme (droplet classes × sprayvolumes). Data underwent analysis of variance and Tukey’s test at 5%, and the correlation between variables was computedconsidering Pearson’s coefficient at 1% and 5%. The VMD of all droplet classes was considerably distinct in the two spray volumes. In both application spray volumes, the minimum VMD for the Coarsedropletclass classification was not attained. The highest means of RS were detectedin the spray volume of 77 L.ha-1. All treatments demonstratedsignificant differences in the analysis of V100 and % drift, with the highest means observed in the Fine droplet (144 L.ha-1) and Medium (77 L.ha-1). A negative correlation existed between VMD × V100 and VMD × % drift and a positive correlation between V100 × % drift in the two spray volumes. RS was negatively correlated with VMD and positively with V100 and % drift, in the spray volume of 77 L.ha-1. In the spray volume of 144 L.ha-1, the correlation between RSand VMD, V100, and % drift was insignificant.
摘要:本研究采用颗粒/液滴成像分析(PDIA)和风洞技术,以阿特拉津和nico硫隆除草剂(2500 + 15 gaiha-1)为混合剂,在77 L.ha-1和144 L.ha-1两种喷雾体积(77 L.ha-1)中,分析了细、中、粗、极粗四种液滴的体积中值直径(VMDs)、相对跨度(RS)、直径小于100 μ m的液滴百分比(V100)和漂移百分比(%漂移)。试验采用完全随机设计,采用4 × 2因子方案(液滴类别×喷雾量)。数据进行方差分析和5%的Tukey检验,并考虑1%和5%的Pearson系数计算变量之间的相关性。在两种喷雾体积中,所有液滴类别的VMD差异很大。在两种应用喷雾体积下,未达到粗滴分类的最小VMD。喷淋量为77 L.ha-1时,RS均值最高。所有处理在V100和%漂移分析上均表现出显著差异,其中细滴(144 L.ha-1)和中滴(77 L.ha-1)的平均值最高。两种喷雾体积的VMD × V100与VMD × %漂移呈负相关,V100 × %漂移呈正相关。在77 L.ha-1喷雾量下,RS与VMD呈负相关,与V100和%漂移呈正相关。在144 L.ha-1的喷雾量下,rr与VMD、V100、%漂移的相关性不显著。
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引用次数: 1
Multidimensional performance assessment of a sample of beef cattle ranches in the Pantanal from a data envelopment analysis perspective 从数据包络分析角度对潘塔纳尔地区肉牛牧场样本进行多维绩效评估
IF 0.8 4区 农林科学 Q3 AGRONOMY Pub Date : 2023-05-26 DOI: 10.1590/0103-8478cr20220595
E. G. Gomes, S. Santos, Emanuel Salgado de Paula, Marcelo Augusto Nogueira, Márcia Divina de Oliveira, S. M. Salis, B. Soriano, W. Tomas
ABSTRACT: Beef cattle ranching is the main economic activity in the Brazilian Pantanal, undertaken in an extensive manner, mostly in natural environments and dynamic in space and time. The ongoing monitoring, assessment, and performance improvement are key aspects of the search for sustainable use of natural resources in beef cattle ranches, as these ranches are the management units in the Pantanal. In this paper we proposed a data envelopment analysis (DEA) approach to assess the performance of fourteen beef cattle ranches in the Pantanal wetland as a case study, considering financial, productive, and environmental sustainability dimensions. We modeled each dimension separately and calculated an overall performance score. We also fit fractional regression models to study the influence of potential covariates on the performance scores. The methodological approach proposed here proved to be adequate for the case study, as the results are in line with the expectations of decision-makers regarding the performance of the ranches. The overall performance analysis was influenced by the proportion of higher quality native forage resources, which depends on the dynamics and on the spatial-temporal variation of the landscapes. Therefore, overall performance requires adaptive management of ranches based on diversity conservation, which benefits the multifunctionality of products and services.
摘要:肉牛养殖是巴西潘塔纳尔地区的主要经济活动,在自然环境中广泛开展,在空间和时间上都是动态的。不断监测、评估和改进业绩是寻求肉牛牧场可持续利用自然资源的关键方面,因为这些牧场是潘塔纳尔的管理单位。本文提出了一种数据包络分析(DEA)方法,以潘塔纳尔湿地14个肉牛牧场为例,从财务、生产和环境可持续性三个维度对其绩效进行评估。我们分别对每个维度建模,并计算总体性能得分。我们还拟合分数回归模型来研究潜在协变量对绩效分数的影响。这里提出的方法方法被证明是适合案例研究的,因为结果符合决策者对牧场业绩的期望。综合性能分析受优质原生牧草资源比例的影响,这取决于景观的动态和时空变化。因此,整体绩效需要基于多样性保护的牧场适应性管理,这有利于产品和服务的多功能性。
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引用次数: 0
Pre slaughter factors in pigs on initial pH and temperature: a case study 猪屠宰前对初始pH值和温度的影响:一个案例研究
IF 0.8 4区 农林科学 Q3 AGRONOMY Pub Date : 2023-05-26 DOI: 10.1590/0103-8478cr20220678
Pollyana Figueiredo Alves de Souza, Amanda Carolina de Souza Amador, F. Bruhn, P. B. Faria
ABSTRACT: This study evaluated factors associated with the pre-slaughter handling of pigs in a commercial slaughterhouse located in Minas Gerais and evaluated the influence of these factors on the initial pH and temperature of the carcasses. In all, 4449 carcasses were evaluated. The factors evaluated were transport distance, transport duration, loading density, fasting duration, resting duration, average weight of the animals, average temperature in the resting area, and maximum temperature in the resting area. The effects of these factors on the initial (i.e., at 45 minutes after slaughter) temperature and pH (pH45) were measured. The pH45 values were used to determine the occurrence of PSE; the carcasses were classified as PSE when they presented a pH45 < 5.90. Results showed that animals fasted for more than 24 hours were more likely to result in PSE meat (1.41 times more likely), as did animals that were exposed to an average temperature above 24 °C (1.52 times more likely). Except for the average weight of the animals, which did not affect the initial temperature values, all other pre-slaughter factors have influenced both pH45 and the initial temperature values, and the difference of fasting duration and the temperature in the resting area increase the incidence of PSE meat.
摘要:本研究评估了米纳斯吉拉斯州一家商业屠宰场宰前处理生猪的相关因素,并评估了这些因素对胴体初始pH值和温度的影响。总共评估了4449具尸体。评价因素为运输距离、运输时间、装载密度、禁食时间、静息时间、动物平均体重、静息区平均温度、静息区最高温度。测量了这些因素对初始(即屠宰后45分钟)温度和pH值(pH45)的影响。pH45值用于判断PSE的发生;pH45 < 5.90时归为PSE。结果显示,禁食超过24小时的动物更有可能导致PSE肉(1.41倍的可能性),暴露在平均温度超过24°C的动物(1.52倍的可能性)也是如此。除动物的平均体重不影响初始温度值外,屠宰前其他因素均对pH45和初始温度值有影响,而禁食时间和休息区域温度的差异增加了PSE肉的发病率。
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引用次数: 0
Resistance of Aeromonas hydrophila isolates to antimicrobials and sanitizers 嗜水气单胞菌对抗菌剂和杀菌剂的耐药性
IF 0.8 4区 农林科学 Q3 AGRONOMY Pub Date : 2023-05-26 DOI: 10.1590/0103-8478cr20220256
Daiane Lima Martins, Andre Cabral, Helen Cristine Leimann Winter, Sandra Mariotto, E. Nascimento, R. Faria, E. L. Martins, D. O. Ritter, M. Lanzarin
ABSTRACT: In recent decades, Aeromonas hydrophila has emerged as a foodborne bacterial pathogen of public health concern, especially as it exhibits resistance to the major chemical sanitizers commonly used in the food industry. Meanwhile, this pathogen may be spread from diseased fish flesh through the contamination of equipment contact surfaces during food processing, thus posing a food safety risk. Thise determined the susceptibility profiles of retail fish-borne A. hydrophila isolates to 24 common antibiotics and five major sanitizers used in the food industry. The polymerase chain reaction technique was used to confirm all A. hydrophila isolates to the species level, and the agar diffusion method was applied to determine the antimicrobial susceptibility profiles. All isolates were confirmed to be A. hydrophila species. This bacterium was observed to have resistance to multiple antibiotics, with the highest resistance index being for those of the beta-lactam class. Additionally, the isolates showed high resistance to four of the five chemical sanitizers tested, with the highest resistance rate being toward sodium hypochlorite. The results suggested that A. hydrophila isolates with multiple resistance to the antimicrobials and main sanitizers used in the food industry can be found in retail fish sold in the Cuiabá region of Mato Grosso, Brazil.
摘要:近几十年来,嗜水气单胞菌已成为一种引起公共卫生关注的食源性细菌病原体,特别是由于它对食品工业中常用的主要化学消毒剂具有耐药性。同时,该病原体可能通过食品加工过程中设备接触面的污染从患病鱼肉传播,从而构成食品安全风险。这确定了零售鱼源性嗜水杆菌分离株对食品工业中使用的24种常见抗生素和5种主要杀菌剂的敏感性。采用聚合酶链反应技术对所有嗜水单胞菌分离株进行种级鉴定,并用琼脂扩散法测定其药敏谱。所有分离株均为嗜水单胞菌。观察到这种细菌对多种抗生素具有耐药性,最高的耐药指数是β -内酰胺类抗生素。此外,分离菌株对5种化学消毒剂中的4种表现出高耐药性,对次氯酸钠的耐药率最高。结果表明,在巴西马托格罗索州cuiab地区销售的零售鱼类中存在对食品工业中使用的抗菌剂和主要杀菌剂具有多重耐药性的嗜水芽胞杆菌分离株。
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引用次数: 2
Physicochemical characterization, phenolic composition and antioxidant activity of genotypes and commercial cultivars of blueberry fruits 蓝莓基因型和商品品种的理化特性、酚类成分和抗氧化活性
IF 0.8 4区 农林科学 Q3 AGRONOMY Pub Date : 2023-05-26 DOI: 10.1590/0103-8478cr20220450
Amanda Radmann Bergmann, T. Siebeneichler, Lucas de Oliveira Fischer, Ígor Ratzmann Holz, C. Rombaldi, Bruna Andressa dos Santos Oliveira, D. L. D. O. Fischer, Catia Silveira da Silva, E. Helbig
ABSTRACT: The physicochemical characterization, antioxidant potential and phenolic composition of fruits of six genotypes (BB3, BB4, BB6, PW1, PW2 and PW5) and seven commercial cultivars (Bluebelle, Bluegem, Briteblue, Climax, Delite, Powderblue and Woodard) were carried out of blueberry, from the 2019/2020 production cycle. Color, soluble solids content, pH, titratable acidity, antioxidant activity (DPPH and ABTS), total phenolic compounds, total flavonoids, total anthocyanins and individual anthocyanins were analyzed. In general, all genotypes and cultivars are rich in phytochemicals. The genotypes PW1, PW2, PW5, BB3 and the cultivars Bluegem, Briteblue, Climax and Delite stand out. Thus, the blueberry genotypes in this study are considered as promising for the food industry and also for fresh consumption as commercial cultivars already consolidated in the fruit market, in view of the excellent phenolic composition present in these small fruits.
摘要/ ABSTRACT摘要:从2019/2020生产周期开始,对蓝莓6个基因型(BB3、BB4、BB6、PW1、PW2和PW5)和7个商品品种(Bluebelle、Bluegem、Briteblue、Climax、Delite、Powderblue和Woodard)果实的理化特性、抗氧化活性和酚类成分进行了研究。分析颜色、可溶性固形物含量、pH、可滴定酸度、抗氧化活性(DPPH和ABTS)、总酚类化合物、总黄酮、总花青素和单个花青素。一般来说,所有基因型和品种都富含植物化学物质。基因型PW1、PW2、PW5、BB3和品种Bluegem、Briteblue、Climax和Delite较为突出。因此,鉴于这些小果实中含有优良的酚类成分,本研究中的蓝莓基因型被认为是有希望用于食品工业和新鲜消费的商品品种,这些品种已经在水果市场上得到巩固。
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引用次数: 0
The policy analysis matrix with profit-efficient data: evaluating the profitability of lemon cultivation in Turkey 具有盈利效率数据的政策分析矩阵:评价土耳其柠檬种植的盈利能力
IF 0.8 4区 农林科学 Q3 AGRONOMY Pub Date : 2023-05-26 DOI: 10.1590/0103-8478cr20220518
Osman Sedat Subaşı
ABSTRACT: In this study, the policy analysis matrix (PAM) and data envelopment analysis (DEA) approaches were used to assess lemon producers’ productivity in Mersin, Turkey, as well as the international competitiveness of lemon cultivation within the scope of its production plan. According to the findings, most enterprises are inefficient, and the profitability of lemons improved from 2863.5 USD ha-1 to 6606.0 USD ha-1 with special prices within the framework of profit-maximising production plans. Regarding social prices, an increment from 3500.8 USD ha-1 to 8071.5 USD ha-1 was proposed to create a more sustainable production plan. To retain competitiveness in the Turkish lemon trade, it has been established that enterprises should transition to a more efficient production structure. For this reason, it has been concluded that inefficiencies in using inputs should be eliminated, and the dissemination of technology and advanced applications will make producers more competitive.
摘要:本研究采用政策分析矩阵(PAM)和数据包络分析(DEA)方法,对土耳其梅尔辛地区柠檬生产者的生产效率进行了评估,并在其生产计划范围内对柠檬种植的国际竞争力进行了评估。研究发现,大多数企业效率低下,在利润最大化的生产计划框架下,柠檬的盈利能力从2863.5 USD ha-1提高到6606.0 USD ha-1。在社会价格方面,建议从3500.8 USD ha-1增加到8071.5 USD ha-1,以创建一个更可持续的生产计划。为了保持土耳其柠檬贸易的竞争力,已经确定企业应该过渡到更有效的生产结构。因此,得出的结论是,应消除使用投入方面的低效率,技术和先进应用的传播将使生产者更具竞争力。
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引用次数: 0
First record of Cerchysiella insularis (Howard, 1897) (Hymenoptera: Encyrtidae) parasitizing Lobiopa insularis (Castelnau, 1840) (Coleoptera: Nitidulidae) on strawberries 第一次记录了岛姬蜂(Howard, 1897)(膜翅目:蜂科)寄生在草莓上的岛姬蜂(Castelnau, 1840)(鞘翅目:小蜂科)
IF 0.8 4区 农林科学 Q3 AGRONOMY Pub Date : 2023-05-26 DOI: 10.1590/0103-8478cr20220453
Rayne Baena, A. M. Bischoff, R. C. Zimmermann, Leticia Tamara Maleski, A. Benatto, Marcelo Teixeira Tavares, Joatan Machado Rosa
ABSTRACT: Strawberry is one of the most socially and economically important small fruits in Brazil, with strong demand by the food industry driving high levels of production. However, phytosanitary problems, especially insect damage, can hamper strawberry cultivation. A pest of great concern is the strawberry sap beetle, Lobiopa insularis (Coleoptera: Nitidulidae), which damages the fruits. Chemical control has not been effective against L. insularis, as it makes harvesting and marketing the product unfeasible. Parasitoids have been effectively utilized to control various strawberry pests and support sustainable production. We report the occurrence of the wasp Cerchysiella insularis (Hymenoptera: Encyrtidae) as a parasite of L. insularis on strawberries in São José dos Pinhais, Paraná, Brazil. Samples of strawberries (San Andreas cultivar) attacked by L. insularis were collected from February to March, 2019. Lobiopa insularis larvae were individually placed in rearing pots, fed on San Andreas strawberries, and checked weekly for symptoms of parasitism or parasitoid emergence. Parasitism rates were 33.85% in March and 45.90% in February. The sex ratio varied between 0.88 and 0.86. All parasitized larvae were infested by C. insularis. The natural parasitism rate above 33% and high sex ratio of C. insularis in strawberry crops indicated that it may have the potential in the control of L. insularis.
摘要:在巴西,草莓是一种具有重要社会和经济意义的小水果,食品行业对草莓的强劲需求推动了草莓的高产量。然而,植物检疫问题,特别是虫害,可能会阻碍草莓的种植。草莓汁甲虫(Lobiopa insularis,鞘翅目:Nitidulidae)是危害草莓果实的主要害虫。化学防治对岛乳杆菌没有效果,因为它使产品的收获和销售不可行。拟寄生物已被有效地应用于草莓害虫的防治和可持续生产。报道了在巴西帕拉那省 o jos dos Pinhais地区的草莓上发现岛斑蜂(Cerchysiella insularis)(膜翅目:蜂科)。2019年2 - 3月采集了受岛蚜侵害的草莓(圣安德烈亚斯品种)样品。将岛叶Lobiopa insularis幼虫单独放置在饲养盆中,以圣安德烈亚斯草莓为食,并每周检查寄生症状或寄生蜂的出现。3月和2月寄生率分别为33.85%和45.90%。性别比在0.88到0.86之间。所有被寄生的幼虫均被岛蛾侵染。天然寄生率达33%以上,且在草莓作物中具有较高的性比,表明其具有防治小蠹的潜力。
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引用次数: 0
Molecular identification of pathogenic Escherichia coli virulence genes and histopathological analysis in chickens condemned for airsacculitis 空气性囊炎鸡致病性大肠杆菌毒力基因的分子鉴定及组织病理学分析
IF 0.8 4区 农林科学 Q3 AGRONOMY Pub Date : 2023-05-15 DOI: 10.1590/0103-8478cr20220383
Mylla Christy da Silva Dufossé, Joelson Sousa Lima, Ana Paula Presley Oliveira Sampaio, Adrianne Maria Brito Pinheiro da Rosa, Josyane Brasil da Silva, C. M. Moraes, T. Roos
ABSTRACT: The present study identified virulence genes and pathological changes caused by Escherichia coli in chicken carcasses condemned for airsacculitis and assessed if the histopathological examination and polymerase chain reaction (PCR) were effective for studies like this. Trachea, liver, and lung were collected from 30 chickens with suspected airsacculitis that has been condemned in the inspection line. The samples were analyzed by PCR to simultaneously identify two virulence genes (iss and tsh genes) and for histopathological testing. PCR efficiently genotypically characterize the E. coli isolates, where the virulence genes iss and tsh were found in three birds simultaneously. The histopathological examination detected a predominance of heterophils and mononuclear cells in the trachea (100%), lung (90%), and liver (13.3%). The liver was the organ where practically no alteration was diagnosed. The results of multiplex PCR for the tsh and iss virulence genes indicate the great potential of the approach in the characterization of E. coli isolates. Unspecific identification did not occur, thus making it necessary to use technologies for the identification and prevention of this agent in aviaries and poultry abattoirs.
摘要:本研究鉴定了空气性囊炎鸡尸体中大肠杆菌引起的毒力基因和病理变化,并评估了组织病理学检查和聚合酶链反应(PCR)是否有效。从30只疑似空气囊炎的鸡身上采集了气管、肝脏和肺,这些鸡在检查线上被谴责。采用PCR同时鉴定两种毒力基因(iss和tsh基因)并进行组织病理学检测。PCR有效地表征了大肠杆菌分离株的基因典型特征,其中在三只鸟中同时发现了毒力基因iss和tsh。组织病理学检查发现气管(100%)、肺(90%)和肝脏(13.3%)以嗜中性粒细胞和单核细胞为主。肝脏是几乎没有被诊断出病变的器官。tsh和iss毒力基因的多重PCR结果表明,该方法在大肠杆菌分离株的鉴定中具有很大的潜力。未发生非特异性鉴定,因此有必要在鸟舍和家禽屠宰场使用鉴定和预防该剂的技术。
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引用次数: 0
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