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Basic design of artificial membrane-less organelles using condensation-prone proteins in plant cells 利用植物细胞中易凝结的蛋白质进行人工无膜细胞器的基本设计。
IF 5.2 1区 生物学 Q1 BIOLOGY Pub Date : 2024-10-26 DOI: 10.1038/s42003-024-07102-8
Yoshito Koja, Takuya Arakawa, Yusuke Yoritaka, Yu Joshima, Hazuki Kobayashi, Kenta Toda, Shin Takeda
Membrane-less organelles, formed by the condensation of biomolecules, play a pivotal role in eukaryotes. Artificial membrane-less organelles and condensates are effective tools for the creation of new cellular functions. However, it is poorly understood how to control the properties that affect condensate function, particularly in plants. Here, we report the construction of model artificial condensates using the condensation-prone proteins OsJAZ2 and AtFCA in a transient assay using rice (Oryza sativa) cells, and how condensate properties, such as subcellular localization, protein mobility, and size can be altered. We showed that proteins of interest can be recruited to condensates using nanobodies or chemically induced dimerization. Furthermore, by combining two types of condensation-prone proteins, we demonstrated that artificial hybrid condensates with heterogeneous material properties could be constructed. Finally, we showed that modified artificial condensates can be constructed in transgenic Arabidopsis thaliana plants. These results provide a framework for the basic design of synthetic membrane-less organelles in plants. Studies using condensation-prone proteins suggest that it is possible to construct and combine artificial condensates with various properties in plant cells, providing a framework for the basic design of synthetic membrane-less organelles in plants.
由生物大分子缩聚而成的无膜细胞器在真核生物中发挥着举足轻重的作用。人造无膜细胞器和凝聚体是创造新细胞功能的有效工具。然而,人们对如何控制影响凝聚态功能的特性知之甚少,尤其是在植物中。在这里,我们报告了在使用水稻(Oryza sativa)细胞进行的瞬时试验中,利用易凝集蛋白 OsJAZ2 和 AtFCA 构建人工凝集素模型的情况,以及如何改变凝集素的特性,如亚细胞定位、蛋白流动性和大小。我们的研究表明,相关蛋白质可以通过纳米抗体或化学诱导二聚化的方式被招募到凝聚体中。此外,通过结合两种易凝结的蛋白质,我们证明可以构建具有异质材料特性的人工混合凝结物。最后,我们证明可以在转基因拟南芥植物中构建改良的人工凝聚体。这些结果为植物合成无膜细胞器的基本设计提供了一个框架。
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引用次数: 0
Endogenous opioid receptor system mediates costly altruism in the human brain 内源性阿片受体系统在人脑中介导代价高昂的利他主义。
IF 5.2 1区 生物学 Q1 BIOLOGY Pub Date : 2024-10-26 DOI: 10.1038/s42003-024-07084-7
Jinglu Chen, Vesa Putkinen, Kerttu Seppälä, Jussi Hirvonen, Kalliopi Ioumpa, Valeria Gazzola, Christian Keysers, Lauri Nummenmaa
Functional neuroimaging studies suggest that a large-scale brain network transforms others’ pain into its vicarious representation in the observer, potentially modulating helping behavior. However, the neuromolecular basis of individual differences in vicarious pain and helping is poorly understood. We investigated the role of the endogenous μ-opioid receptor (MOR) system in altruistic costly helping. MOR density was measured using [11C]carfentanil. In a separate fMRI experiment, participants could donate money to reduce a confederate’s pain from electric shocks. Participants were generally willing to help, and brain activity was observed in amygdala, anterior insula, anterior cingulate cortex (ACC), striatum, primary motor cortex, primary somatosensory cortex and thalamus when witnessing others’ pain. Haemodynamic responses were negatively associated with MOR availability in emotion circuits. However, MOR availability positively associated with the ACC and hippocampus during helping. These findings suggest that the endogenous MOR system modulates altruism in the human brain. This PET-fMRI fusion study reveals that endogenous u-opioid receptor system modulates altruistic brain activity and its individual differences.
功能神经影像学研究表明,一个大规模的大脑网络会将他人的痛苦转化为观察者的代入表征,从而有可能调节帮助行为。然而,人们对替代疼痛和帮助行为的个体差异的神经分子基础知之甚少。我们研究了内源性μ-阿片受体(MOR)系统在利他主义成本帮助中的作用。我们使用[11C]卡芬太尼测量了MOR的密度。在另一个 fMRI 实验中,参与者可以捐钱来减轻同伴的电击疼痛。参与者普遍愿意提供帮助,在目睹他人疼痛时,杏仁核、前岛叶、前扣带回皮层(ACC)、纹状体、初级运动皮层、初级体感皮层和丘脑都出现了大脑活动。血流动力学反应与情绪回路中的MOR可用性呈负相关。然而,在帮助过程中,MOR的可用性与ACC和海马呈正相关。这些研究结果表明,内源性MOR系统能调节人脑中的利他主义。
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引用次数: 0
Inverse relation between motion perception and postural responses induced by motion of a touched object 运动感知与被触物体运动引起的姿势反应之间的反比关系
IF 5.2 1区 生物学 Q1 BIOLOGY Pub Date : 2024-10-26 DOI: 10.1038/s42003-024-07093-6
Shinya Takamuku, Beata Struckova, Matthew J. Bancroft, Hiroaki Gomi, Patrick Haggard, Diego Kaski
Self vs. external attribution of motions based on vestibular cues is suggested to underlie our coherent perception of object motion and self-motion. However, it remains unclear whether such attribution also underlies sensorimotor responses. Here, we examined this issue in the context of touch. We asked participants to lightly touch a moving object with their thumb while standing still on an unstable surface. We measured both the accuracy of judging the object motion direction and the postural response. If the attribution underlies both object-motion perception and posture control, sensitivity of posture to object motion should decrease with motion speed since high speed motion is unlikely to reflect self-motion. Furthermore, when motion perception is erroneous, there should be a corresponding increase in postural responses. Our results are consistent with these predictions and suggest that self-external attribution of somatosensory motion underlies both object motion perception and postural responses. Simultaneous measurements of postural response and motion perception triggered by touched surface motion reveal a process of self-external attribution of somatosensory motion signals that underlies motor and perceptual processes
基于前庭线索的自我与外部运动归因被认为是我们对物体运动和自我运动的连贯感知的基础。然而,这种归因是否也是感觉运动反应的基础仍不清楚。在这里,我们以触摸为背景研究了这一问题。我们要求参与者在不稳定的表面上站立不动时,用拇指轻触一个移动的物体。我们同时测量了对物体运动方向判断的准确性和姿势反应。如果归因是物体运动感知和姿势控制的基础,那么姿势对物体运动的敏感性就会随着运动速度的增加而降低,因为高速运动不太可能反映自我运动。此外,当运动感知出现错误时,姿势反应也会相应增加。我们的研究结果与这些预测一致,表明体感运动的自我外部归因是物体运动感知和姿势反应的基础。
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引用次数: 0
Autoregulation ensures vertical transmission of the linear prophage GIL01 自动调节确保了线性噬菌体 GIL01 的垂直传播。
IF 5.2 1区 生物学 Q1 BIOLOGY Pub Date : 2024-10-25 DOI: 10.1038/s42003-024-07082-9
Anja Pavlin, Nadine Fornelos, Maja Popović, Neža Praček, Gregor Bajc, Margarita Salas, Matej Butala
Betatectiviruses are prophages consisting of linear extrachromosomal genomes without obvious plasmid modules. It remains unclear how betatectiviruses are maintained in low-copy numbers in host cells and how they are vertically transmitted. Phage GIL01 is a model betatectivirus that infects the mosquito pathogen Bacillus thuringiensis serovar israelensis. Previous studies identified two closely spaced promoters, P1 and P2, responsible for the expression of GIL01 genes required for prophage replication and the switch from the lysogenic to lytic cycle. Here, we report that the GIL01-encoded 58-amino acid long gp1 protein forms a large nucleoprotein complex that represses its transcription from the strong promoter P2. Notably, ectopic expression of gp1 resulted in the loss of GIL01 in exponential cultures and immunized cells against infection with GIL01, indicating that gp1 plays a repressive role in the phage cycle. This finding is consistent with mutations in gp1 committing GIL01 to the lytic cycle and we show that maintenance of this phage variant in the bacterial population is contingent on the accumulation of deletions in the P1-P2 region. The fact that gp1 is conserved across most sequenced betatectiviruses suggests that the regulatory mechanism of gp1 that controls prophage maintenance is widespread among these bacteriophages. Gp1, a 58-amino acid DNA-binding protein encoded by betatectivirus GIL01, is responsible for maintenance of the GIL01 linear prophage genome in host Bacillus thuringiensis serovar israelensis.
Betatectiviruses 是由线性染色体外基因组组成的原生病毒,没有明显的质粒模块。目前仍不清楚倍他病毒如何在宿主细胞中保持低拷贝数,以及如何垂直传播。噬菌体 GIL01 是一种感染蚊子病原体苏云金芽孢杆菌(Bacillus thuringiensis serovar israelensis)的betatectivirus模型。以前的研究发现了两个间隔很近的启动子 P1 和 P2,它们负责表达原噬菌体复制和从溶解循环到溶解循环所需的 GIL01 基因。在这里,我们报告了由 GIL01 编码的 58 氨基酸长 gp1 蛋白形成了一个大型核蛋白复合物,该复合物抑制了强启动子 P2 的转录。值得注意的是,gp1 的异位表达会导致指数培养物中 GIL01 的缺失,并使细胞免受 GIL01 的感染,这表明 gp1 在噬菌体循环中起抑制作用。这一发现与 gp1 的突变使 GIL01 进入溶菌周期相一致,而且我们还发现这种噬菌体变体在细菌群体中的维持取决于 P1-P2 区域缺失的积累。gp1 在大多数已测序的 betatectiviruses 中是保守的,这一事实表明 gp1 控制噬菌体维持的调节机制在这些噬菌体中是普遍存在的。
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引用次数: 0
Temporal coherence shapes cortical responses to speech mixtures in a ferret cocktail party 在雪貂鸡尾酒会上,时间连贯性决定了大脑皮层对混合语音的反应。
IF 5.2 1区 生物学 Q1 BIOLOGY Pub Date : 2024-10-25 DOI: 10.1038/s42003-024-07096-3
Neha Joshi, Wing Yiu Ng, Karan Thakkar, Daniel Duque, Pingbo Yin, Jonathan Fritz, Mounya Elhilali, Shihab Shamma
Perceptual segregation of complex sounds such as speech and music simultaneously emanating from multiple sources is a remarkable ability that is common in humans and other animals alike. Unlike animal physiological experiments with simplified sounds or human investigations with spatially broad imaging techniques, this study combines insights from animal single-unit recordings with segregation of speech-like sound mixtures. Ferrets are trained to attend to a female voice and detect a target word, both in presence and absence of a concurrent equally salient male voice. Recordings are made in primary and secondary auditory cortical fields, and in frontal cortex. During task performance, representation of the female words becomes enhanced relative to the male in all, but especially in higher cortical regions. Analysis of the temporal and spectral response characteristics during task performance reveals how speech segregation gradually emerges in the auditory cortex. A computational model evaluated on the same voice mixtures replicates and extends these results to different attentional targets (attention to female or male voices). These findings underscore the role of the principle of temporal coherence whereby attention to a target voice binds together all neural responses coherently modulated with the target, thus ultimately forming and extracting a common auditory stream. Ferrets learned to attend and react to words of a female voice embedded in a speech mixture with a male. Recordings in auditory cortex reveal rapidly enhanced responses to the attended female voice and a simultaneous suppression of the male distractor.
对同时来自多个声源的复杂声音(如语音和音乐)进行感知分离是一种非凡的能力,人类和其他动物都具有这种能力。与使用简化声音进行的动物生理实验或使用空间成像技术进行的人类研究不同,本研究将动物单细胞记录与语音类声音混合物的分离相结合。训练雪貂在有或没有同样突出的雄性声音的情况下注意雌性声音并检测目标词。在初级和次级听觉皮层区域以及额叶皮层进行记录。在任务执行过程中,相对于男声,女声单词在所有皮层区域的表征都会增强,尤其是在高级皮层区域。对任务执行过程中时间和频谱反应特征的分析揭示了语音分离是如何在听觉皮层中逐渐出现的。对相同声音混合物进行评估的计算模型将这些结果复制并扩展到了不同的注意目标(注意女声或男声)。这些发现强调了时间连贯性原理的作用,即对目标声音的注意会将所有与目标声音连贯调制的神经反应结合在一起,从而最终形成并提取出共同的听觉流。
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引用次数: 0
Inflammation impacts androgen receptor signaling in basal prostate stem cells through interleukin 1 receptor antagonist 炎症通过白细胞介素 1 受体拮抗剂影响基础前列腺干细胞的雄激素受体信号转导。
IF 5.2 1区 生物学 Q1 BIOLOGY Pub Date : 2024-10-25 DOI: 10.1038/s42003-024-07071-y
Paula O. Cooper, Jiang Yang, Hsing-Hui Wang, Meaghan M. Broman, Shyaman Madhawa Jayasundara, Subhransu Sekhar Sahoo, Bingyu Yan, Gada D. Awdalkreem, Gregory M. Cresswell, Liang Wang, Emery Goossens, Nadia A. Lanman, Rebecca W. Doerge, Faye Zheng, Liang Cheng, Saeed Alqahtani, Scott A. Crist, Robert E. Braun, Majid Kazemian, Travis J. Jerde, Timothy L. Ratliff
Chronic prostate inflammation in patients with benign prostate hyperplasia (BPH) correlates with the severity of symptoms. How inflammation contributes to prostate enlargement and/or BPH symptoms and the underlying mechanisms remain unclear. In this study, we utilize a unique transgenic mouse model that mimics chronic non-bacterial prostatitis in men and investigate the impact of inflammation on androgen receptor (AR) in basal prostate stem cells (bPSC) and their differentiation in vivo. We find that inflammation significantly enhances AR levels and activity in bPSC. More importantly, we identify interleukin 1 receptor antagonist (IL-1RA) as a crucial regulator of AR in bPSC during inflammation. IL-1RA is one of the top molecules upregulated by inflammation, and inhibiting IL-1RA reverses the enhanced AR activity in organoids derived from inflamed bPSC. Additionally, IL-1RA appears to activate AR by counteracting IL-1α''s inhibitory effect. Furthermore, using a lineage tracing model, we observe that inflammation induces bPSC proliferation and differentiation into luminal cells even under castrate conditions, indicating that AR activation driven by inflammation is sufficient to promote bPSC proliferation and differentiation. Taken together, our study uncovers mechanisms through which inflammation modulates AR signaling in bPSC and induces bPSC luminal differentiation that may contribute to prostate hyperplasia. Inflammation enhances androgen receptor levels and activity in basal prostate stem cells via IL-1RA, promoting their proliferation and differentiation, potentially contributing to benign prostate hyperplasia in mice.
良性前列腺增生症(BPH)患者的慢性前列腺炎症与症状的严重程度相关。炎症是如何导致前列腺增生和/或良性前列腺增生症状的,其根本机制尚不清楚。在这项研究中,我们利用独特的转基因小鼠模型模拟男性慢性非细菌性前列腺炎,并研究炎症对基底前列腺干细胞(bPSC)中雄激素受体(AR)及其体内分化的影响。我们发现,炎症会明显提高基底前列腺干细胞中的AR水平和活性。更重要的是,我们发现白细胞介素1受体拮抗剂(IL-1RA)是炎症期间碱性前列腺干细胞中AR的关键调节因子。IL-1RA是炎症上调的主要分子之一,抑制IL-1RA可逆转发炎bPSC衍生的器官组织中增强的AR活性。此外,IL-1RA似乎通过抵消IL-1α的抑制作用来激活AR。此外,我们还利用一个系谱追踪模型观察到,即使在阉割条件下,炎症也能诱导bPSC增殖并分化为管腔细胞,这表明炎症驱动的AR激活足以促进bPSC增殖和分化。综上所述,我们的研究揭示了炎症调节bPSC中AR信号转导和诱导bPSC管腔分化的机制,这可能会导致前列腺增生。
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引用次数: 0
miR-124 coordinates metabolic regulators acting at early stages of human neurogenesis miR-124 在人类神经发生的早期阶段协调代谢调节因子的作用。
IF 5.2 1区 生物学 Q1 BIOLOGY Pub Date : 2024-10-25 DOI: 10.1038/s42003-024-07089-2
Geurim Son, Yongwoo Na, Yongsung Kim, Ji-Hoon Son, Gregory D. Clemenson, Simon T. Schafer, Jong-Yeon Yoo, Sarah L. Parylak, Apua Paquola, Hyunsu Do, Dayeon Kim, Insook Ahn, Mingyu Ju, Chanhee S. Kang, Younghee Ju, Eunji Jung, Aidan H. McDonald, Youngjin Park, Gilhyun Kim, Se-Bum Paik, Junho Hur, Joon Kim, Yong-Mahn Han, Seung-Hee Lee, Fred H. Gage, Jong-Seo Kim, Jinju Han
Metabolic dysregulation of neurons is associated with diverse human brain disorders. Metabolic reprogramming occurs during neuronal differentiation, but it is not fully understood which molecules regulate metabolic changes at the early stages of neurogenesis. In this study, we report that miR-124 is a driver of metabolic change at the initiating stage of human neurogenesis. Proteome analysis has shown the oxidative phosphorylation pathway to be the most significantly altered among the differentially expressed proteins (DEPs) in the immature neurons after the knockdown of miR-124. In agreement with these proteomics results, miR-124-depleted neurons display mitochondrial dysfunctions, such as decreased mitochondrial membrane potential and cellular respiration. Moreover, morphological analyses of mitochondria in early differentiated neurons after miR-124 knockdown result in smaller and less mature shapes. Lastly, we show the potential of identified DEPs as novel metabolic regulators in early neuronal development by validating the effects of GSTK1 on cellular respiration. GSTK1, which is upregulated most significantly in miR-124 knockdown neurons, reduces the oxygen consumption rate of neural cells. Collectively, our data highlight the roles of miR-124 in coordinating metabolic maturation at the early stages of neurogenesis and provide insights into potential metabolic regulators associated with human brain disorders characterized by metabolic dysfunctions. miR-124 as a driver of metabolic change initiating neurogenesis by governing the mitochondrial oxidative phosphorylation pathway.
神经元的代谢失调与多种人类脑部疾病有关。代谢重编程发生在神经元分化过程中,但目前还不完全清楚是哪些分子调控神经发生早期阶段的代谢变化。在这项研究中,我们报告了 miR-124 是人类神经发生初始阶段代谢变化的驱动因素。蛋白质组分析表明,在敲除 miR-124 后的未成熟神经元中,氧化磷酸化途径是差异表达蛋白(DEPs)中变化最显著的。与这些蛋白质组学结果一致,miR-124 缺失的神经元显示出线粒体功能障碍,如线粒体膜电位和细胞呼吸下降。此外,miR-124 被敲除后,早期分化神经元中线粒体的形态学分析结果显示,线粒体的形状更小、更不成熟。最后,我们通过验证 GSTK1 对细胞呼吸的影响,展示了已发现的 DEPs 作为早期神经元发育过程中新型代谢调节因子的潜力。在 miR-124 敲除的神经元中,GSTK1 的上调最为显著,它降低了神经细胞的耗氧量。总之,我们的数据突显了 miR-124 在神经发生早期阶段协调代谢成熟的作用,并提供了与以代谢功能障碍为特征的人类脑部疾病相关的潜在代谢调节因子的见解。
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引用次数: 0
Comprehensive evaluation and prediction of editing outcomes for near-PAMless adenine and cytosine base editors 全面评估和预测近无 PAM 腺嘌呤和胞嘧啶碱基编辑器的编辑结果。
IF 5.2 1区 生物学 Q1 BIOLOGY Pub Date : 2024-10-25 DOI: 10.1038/s42003-024-07078-5
Xiaoyu Zhou, Jingjing Gao, Liheng Luo, Changcai Huang, Jiayu Wu, Xiaoyue Wang
Base editors enable the direct conversion of target bases without inducing double-strand breaks, showing great potential for disease modeling and gene therapy. Yet, their applicability has been constrained by the necessity for specific protospacer adjacent motif (PAM). We generate four versions of near-PAMless base editors and systematically evaluate their editing patterns and efficiencies using an sgRNA-target library of 45,747 sequences. Near-PAMless base editors significantly expanded the targeting scope, with both PAM and target flanking sequences as determinants for editing outcomes. We develop BEguider, a deep learning model, to accurately predict editing results for near-PAMless base editors. We also provide experimentally measured editing outcomes of 20,541 ClinVar sites, demonstrating that variants previously inaccessible by NGG PAM base editors can now be precisely generated or corrected. We make our predictive tool and data available online to facilitate development and application of near-PAMless base editors in both research and clinical settings. Systematic evaluation of near-PAMless base editors enables a robust deep learning model for predicting editing outcomes, facilitating broader and more precise targeting of disease-associated variants for research and therapy.
碱基编辑器能直接转换目标碱基,而不会引起双链断裂,在疾病建模和基因治疗方面具有巨大潜力。然而,它们的适用性一直受到特定原间隔相邻基序(PAM)的限制。我们利用一个包含 45,747 条序列的 sgRNA 目标文库生成了四种版本的近无 PAM 碱基编辑器,并系统地评估了它们的编辑模式和效率。近无 PAM 碱基编辑器显著扩大了靶向范围,PAM 和靶侧翼序列都是编辑结果的决定因素。我们开发了深度学习模型 BEguider,以准确预测近无 PAM 碱基编辑器的编辑结果。我们还提供了 20,541 个 ClinVar 位点的实验测量编辑结果,证明以前 NGG PAM 碱基编辑器无法访问的变体现在可以精确生成或校正。我们在线提供我们的预测工具和数据,以促进研究和临床环境中近无 PAM 碱基编辑器的开发和应用。
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引用次数: 0
A conserved Plasmodium nuclear protein is critical for late liver stage development 一种保守的疟原虫核蛋白对肝脏晚期发育至关重要。
IF 5.2 1区 生物学 Q1 BIOLOGY Pub Date : 2024-10-25 DOI: 10.1038/s42003-024-07063-y
Debashree Goswami, Silvia A. Arredondo, William Betz, Janna Armstrong, Sudhir Kumar, Gigliola Zanghi, Hardik Patel, Nelly Camargo, Kenza M. Z. Oualim, Annette M. Seilie, Sophia Schneider, Sean C. Murphy, Stefan H. I. Kappe, Ashley M. Vaughan
Malaria, caused by Plasmodium parasites, imposes a significant health burden and live-attenuated parasites are being pursued as vaccines. Here, we report on the creation of a genetically attenuated parasite by the deletion of Plasmodium LINUP, encoding a liver stage nuclear protein. In the rodent parasite Plasmodium yoelii, LINUP expression was restricted to liver stage nuclei after the onset of liver stage schizogony. Compared to wildtype P. yoelii, P. yoelii LINUP gene deletion parasites (linup—) exhibited no phenotype in blood stages and mosquito stages but suffered developmental arrest late in liver stage schizogony with a pronounced defect in exo-erythrocytic merozoite formation. This defect caused severe attenuation of the liver stage-to-blood stage transition and immunization of mice with linup — parasites conferred robust protection against infectious sporozoite challenge. LINUP gene deletion in the human parasite Plasmodium falciparum also caused a severe defect in late liver stage differentiation. Importantly, P. falciparum linup — liver stages completely failed to transition from the liver stage to a viable blood stage infection in a humanized mouse model. These results suggest that P. falciparum LINUP is an ideal target for late liver stage attenuation that can be incorporated into a late liver stage-arresting replication competent whole parasite vaccine. A conserved Plasmodium protein, specific to the liver stage and localized in the nucleus of liver stage schizonts, plays a critical role in liver stage development and differentiation.
疟疾是由疟原虫引起的,给人们的健康造成了巨大的负担,目前正在研究将减毒活寄生虫作为疫苗。在这里,我们报告了通过删除疟原虫 LINUP(编码肝脏阶段的核蛋白)来创建基因减毒寄生虫的情况。在啮齿类寄生虫疟原虫yoelii中,LINUP的表达仅限于肝分裂期开始后的肝阶段细胞核。与野生型疟原虫相比,疟原虫 LINUP 基因缺失寄生虫(linup-)在血期和蚊期没有表现出任何表型,但在肝期分裂后期发育停滞,外红细胞裂殖体形成明显缺陷。这种缺陷导致肝阶段向血阶段过渡的过程严重减弱,用 linup - 寄生虫免疫小鼠可对传染性孢子虫挑战产生强大的保护作用。人类寄生虫恶性疟原虫的 LINUP 基因缺失也会导致肝脏后期分化的严重缺陷。重要的是,在人源化小鼠模型中,恶性疟原虫 LINUP - 肝阶段完全不能从肝阶段过渡到有活力的血阶段感染。这些结果表明,恶性疟原虫 LINUP 是肝晚期衰减的理想靶标,可以将其纳入肝晚期抑制复制的全寄生虫疫苗中。
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引用次数: 0
The need for high-resolution gut microbiome characterization to design efficient strategies for sustainable aquaculture production 需要高分辨率的肠道微生物组特征描述,以设计可持续水产养殖生产的高效战略。
IF 5.2 1区 生物学 Q1 BIOLOGY Pub Date : 2024-10-25 DOI: 10.1038/s42003-024-07087-4
Shashank Gupta, Arturo Vera-Ponce de León, Miyako Kodama, Matthias Hoetzinger, Cecilie G. Clausen, Louisa Pless, Ana R. A. Verissimo, Bruno Stengel, Virginia Calabuig, Renate Kvingedal, Stanko Skugor, Bjørge Westereng, Thomas Nelson Harvey, Anna Nordborg, Stefan Bertilsson, Morten T. Limborg, Turid Mørkøre, Simen R. Sandve, Phillip B. Pope, Torgeir R. Hvidsten, Sabina Leanti La Rosa
Microbiome-directed dietary interventions such as microbiota-directed fibers (MDFs) have a proven track record in eliciting responses in beneficial gut microbes and are increasingly being promoted as an effective strategy to improve animal production systems. Here we used initial metataxonomic data on fish gut microbiomes as well as a wealth of a priori mammalian microbiome knowledge on α-mannooligosaccharides (MOS) and β-mannan-derived MDFs to study effects of such feed supplements in Atlantic salmon (Salmo salar) and their impact on its gut microbiome composition and functionalities. Our multi-omic analysis revealed that the investigated MDFs (two α-mannans and an acetylated β-galactoglucomannan), at a dose of 0.2% in the diet, had negligible effects on both host gene expression, and gut microbiome structure and function under the studied conditions. While a subsequent trial using a higher (4%) dietary inclusion of β-mannan significantly shifted the gut microbiome composition, there were still no biologically relevant effects on salmon metabolism and physiology. Only a single Burkholderia-Caballeronia-Paraburkholderia (BCP) population demonstrated consistent and significant abundance shifts across both feeding trials, although with no evidence of β-mannan utilization capabilities or changes in gene transcripts for producing metabolites beneficial to the host. In light of these findings, we revisited our omics data to predict and outline previously unreported and potentially beneficial endogenous lactic acid bacteria that should be targeted with future, conceivably more suitable, MDF strategies for salmon. A multi-omic approach enables the reconstruction of microbial metabolic dynamics in the salmon gut in response to feed and feed supplements, outlining novel and potentially beneficial strategies to manipulate the salmon gut microbiota.
微生物定向膳食干预措施,如微生物定向纤维(MDFs),在激发有益肠道微生物的反应方面有着良好的记录,并被越来越多地推广为改善动物生产系统的有效策略。在这里,我们利用鱼类肠道微生物组的初步元分类数据以及哺乳动物微生物组关于α-甘露寡糖(MOS)和β-甘露聚糖衍生的 MDFs 的大量先验知识,研究大西洋鲑鱼(Salmo salar)中此类饲料添加剂的效果及其对肠道微生物组组成和功能的影响。我们的多组学分析表明,所研究的 MDF(两种 α-甘露聚糖和一种乙酰化 β-半乳甘露聚糖)在日粮中的剂量为 0.2%,在研究条件下对宿主基因表达以及肠道微生物组结构和功能的影响微乎其微。虽然在随后的试验中,β-甘露聚糖的膳食添加量提高(4%)后,肠道微生物组的组成发生了显著变化,但对鲑鱼的新陈代谢和生理学仍没有产生生物相关影响。在两次喂养试验中,只有一个伯克霍尔德氏菌-卡巴勒氏菌-帕拉伯克霍尔德氏菌(BCP)种群表现出一致且显著的丰度变化,但没有证据表明其具有利用β-甘露聚糖的能力,或产生对宿主有益代谢物的基因转录本发生了变化。鉴于这些发现,我们重新研究了我们的 omics 数据,以预测和概述以前未报道的、可能对鲑鱼有益的内源乳酸菌。
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