Ivana Šagud, A. Ratković, Matea Cedilak, M. Bosnar, Vanja Kelava, I. Škorić
Biological activity of naphthoxazoles, fused hetero-benzoxazoles and benzobicyclo[3.2.1]-derivatives was investigated in proliferation and inflammation based assays. The tested compounds were prepared by photocylization or photocycloaddition reactions. Effect of compounds on proliferation of several cancer cell lines was determined by measuring cell metabolic activity through time. Lipopolysaccharide (LPS) stimulation of peripheral blood mononuclear cells (PBMC) was used to investigate antiinflammatory properties of the compounds. Several naphthoxazoles and fused hetero-benzoxazoles inhibited TNFα protein expression in LPS stimulated PBMC, indicating possible antiinflammatory role which would be interesting to further investigate. Physico-chemical properties of tested compounds have been also studied using chromatographic lipophilicity measure, chrom logD and logP was calculated as the importance of physico-chemical properties of compounds at early stage of discovery of new drugs is well established. The similarities in structure and activity of some representative compounds affirm the need to further address their antiinflammatory properties.
{"title":"Antiinflammatory and Antiproliferative Activity of Naphthoxazole, Fused Hetero-benzoxazole and Bridged Benzobicyclic Photoproducts","authors":"Ivana Šagud, A. Ratković, Matea Cedilak, M. Bosnar, Vanja Kelava, I. Škorić","doi":"10.5562/cca3530","DOIUrl":"https://doi.org/10.5562/cca3530","url":null,"abstract":"Biological activity of naphthoxazoles, fused hetero-benzoxazoles and benzobicyclo[3.2.1]-derivatives was investigated in proliferation and inflammation based assays. The tested compounds were prepared by photocylization or photocycloaddition reactions. Effect of compounds on proliferation of several cancer cell lines was determined by measuring cell metabolic activity through time. Lipopolysaccharide (LPS) stimulation of peripheral blood mononuclear cells (PBMC) was used to investigate antiinflammatory properties of the compounds. Several naphthoxazoles and fused hetero-benzoxazoles inhibited TNFα protein expression in LPS stimulated PBMC, indicating possible antiinflammatory role which would be interesting to further investigate. Physico-chemical properties of tested compounds have been also studied using chromatographic lipophilicity measure, chrom logD and logP was calculated as the importance of physico-chemical properties of compounds at early stage of discovery of new drugs is well established. The similarities in structure and activity of some representative compounds affirm the need to further address their antiinflammatory properties.","PeriodicalId":10822,"journal":{"name":"Croatica Chemica Acta","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2019-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5562/cca3530","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46474003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Among the major components of the Golgi apparatus are the GRASP family proteins, including GRASP65 on the cis-Golgi side. With its GRASP domain, GRASP65 is involved in Golgi stacking and ribbon formation. Interaction of GRASP65 with the Golgi marker protein GM130 is important for the docking of vesicles to the Golgi membrane. We present here structures of the two individual PDZ domains comprising the GRASP domain in human GRASP65. We use isothermal titration calorimetry to probe the interaction between GRASP65 and GM130. Additionally, we present evidence for the limited sequence conservation of the PDZ fold by describing the PDZ domain structure of the GRASP65 homolog Grh1 from Saccharomyces cerevisiae.
{"title":"Crystal Structures of the Single PDZ Domains from GRASP65 and their Interaction with the Golgin GM130","authors":"C. M. Jurk, Y. Roske, U. Heinemann","doi":"10.5562/CCA3341","DOIUrl":"https://doi.org/10.5562/CCA3341","url":null,"abstract":"Among the major components of the Golgi apparatus are the GRASP family proteins, including GRASP65 on the cis-Golgi side. With its GRASP domain, GRASP65 is involved in Golgi stacking and ribbon formation. Interaction of GRASP65 with the Golgi marker protein GM130 is important for the docking of vesicles to the Golgi membrane. We present here structures of the two individual PDZ domains comprising the GRASP domain in human GRASP65. We use isothermal titration calorimetry to probe the interaction between GRASP65 and GM130. Additionally, we present evidence for the limited sequence conservation of the PDZ fold by describing the PDZ domain structure of the GRASP65 homolog Grh1 from Saccharomyces cerevisiae.","PeriodicalId":10822,"journal":{"name":"Croatica Chemica Acta","volume":"91 1","pages":"255-264"},"PeriodicalIF":0.3,"publicationDate":"2019-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44655988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Bušić, K. Vrandečić, Tamara Siber, S. Roca, D. Vikić-Topić, D. Gašo-Sokač
Ten novel isonicotinamide derivatives were prepared by quaternization reactions of isonicotinamide with methyl iodide and nine differently substituted 2 bromoacetophenones under rapid microwave irradiation of 10 minutes. The microwave preparations were significantly faster and with yields higher up to 8 times, than the preparations by conventional method. The structures of synthesized molecules were determined by one- and two dimensional NMR and IR spectroscopy, mass spectrometry and elemental analysis. Antifungal activity of all compounds was tested in two different concentrations (10 and 100 µg mL–1) against Fusarium oxysporum, Fusarium culmorum, Macrophomina phaseolina and Sclerotinia sclerotiorum in vitro. From the antifungal assay it can be seen that the most prepared compounds have moderate to weak activity against M. phaseolina and F. culmorum. A very high inhibitory rate was observed against S. sclerotiorum, 62–87.5 % in concentration of 10 µg mL–1 and 83.7–93.2 % in concentration of 100 µg mL–1.
{"title":"A Rapid Microwave Induced Synthesis of Isonicotinamide Derivatives and their Antifungal Activity","authors":"V. Bušić, K. Vrandečić, Tamara Siber, S. Roca, D. Vikić-Topić, D. Gašo-Sokač","doi":"10.5562/CCA3527","DOIUrl":"https://doi.org/10.5562/CCA3527","url":null,"abstract":"Ten novel isonicotinamide derivatives were prepared by quaternization reactions of isonicotinamide with methyl iodide and nine differently substituted 2 bromoacetophenones under rapid microwave irradiation of 10 minutes. The microwave preparations were significantly faster and with yields higher up to 8 times, than the preparations by conventional method. The structures of synthesized molecules were determined by one- and two dimensional NMR and IR spectroscopy, mass spectrometry and elemental analysis. Antifungal activity of all compounds was tested in two different concentrations (10 and 100 µg mL–1) against Fusarium oxysporum, Fusarium culmorum, Macrophomina phaseolina and Sclerotinia sclerotiorum in vitro. From the antifungal assay it can be seen that the most prepared compounds have moderate to weak activity against M. phaseolina and F. culmorum. A very high inhibitory rate was observed against S. sclerotiorum, 62–87.5 % in concentration of 10 µg mL–1 and 83.7–93.2 % in concentration of 100 µg mL–1.","PeriodicalId":10822,"journal":{"name":"Croatica Chemica Acta","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2019-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5562/CCA3527","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44499148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Goran Benković, Hrvoje Rimac, Ž. Maleš, S. Tomić, Zoran Lončar, M. Bojić
One of the most important groups of metabolic enzymes is cytochrome P450 superfamily. These enzymes are important in terms of the catalytic diversity and the large number of xenobiotics that are detoxified or activated by converting to reactive metabolites. Flavonoids are xenobiotics to which humans are exposed through diet. Data on their oxidative metabolism mediated by cytochromes P450 are limited. The aim of this study was to determine the enzymatic kinetics of O-demethylation and aromatic hydroxylation of flavonoid aglycons on recombinant cytochrome P450 enzymes and human liver microsomes systems. The study was performed on ten flavonoids, namely 3,7-dihydroxyflavone, 7-hydroxyflavone, acacetin, apigenin, flavone, galangin, kaempferol, naringenin, sakuranetin, and tangeretin using liquid chromatography coupled with mass spectrometry and UV detector. Most relevant enzyme involved in metabolism of flavonoid aglycons is CYP1A2, and its catalytic effectiveness ranges from 0.5 to 2.9 × 106 M–1 min–1. Having in mind high expression and involvement of CYP1A2 in metabolism of xenobiotics including drugs, and its intraindividual differences in expression and activity, potential of drug-flavonoid competitive interactions/inhibitions should be considered when consuming dietary supplement and foods rich in flavonoids.
{"title":"Characterization of O-demethylations and Aromatic Hydroxylations Mediated by Cytochromes P450 in the Metabolism of Flavonoid Aglycons","authors":"Goran Benković, Hrvoje Rimac, Ž. Maleš, S. Tomić, Zoran Lončar, M. Bojić","doi":"10.5562/CCA3528","DOIUrl":"https://doi.org/10.5562/CCA3528","url":null,"abstract":"One of the most important groups of metabolic enzymes is cytochrome P450 superfamily. These enzymes are important in terms of the catalytic diversity and the large number of xenobiotics that are detoxified or activated by converting to reactive metabolites. Flavonoids are xenobiotics to which humans are exposed through diet. Data on their oxidative metabolism mediated by cytochromes P450 are limited. The aim of this study was to determine the enzymatic kinetics of O-demethylation and aromatic hydroxylation of flavonoid aglycons on recombinant cytochrome P450 enzymes and human liver microsomes systems. The study was performed on ten flavonoids, namely 3,7-dihydroxyflavone, 7-hydroxyflavone, acacetin, apigenin, flavone, galangin, kaempferol, naringenin, sakuranetin, and tangeretin using liquid chromatography coupled with mass spectrometry and UV detector. Most relevant enzyme involved in metabolism of flavonoid aglycons is CYP1A2, and its catalytic effectiveness ranges from 0.5 to 2.9 × 106 M–1 min–1. Having in mind high expression and involvement of CYP1A2 in metabolism of xenobiotics including drugs, and its intraindividual differences in expression and activity, potential of drug-flavonoid competitive interactions/inhibitions should be considered when consuming dietary supplement and foods rich in flavonoids.","PeriodicalId":10822,"journal":{"name":"Croatica Chemica Acta","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2019-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5562/CCA3528","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47098717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Talić, I. Odak, Anita Martinović Bevanda, Nikolina Crnjac, M. Paštar
The chemical composition of essential oils isolated from immortelle (Helicrysum italicum subsp. italicum) collected in Herzegovina during five different periods, was investigated by GC/MS analysis. The main compounds were a-pinene (15.7 %) and γ-curcumene (12.8 %), followed by 4,6,9-trimethyldec-8-en-3,5-dione (8.7 %), neryl acetate (6.9 %), limonene (6.4 %) and β-selinene (5.3 %). In total, 69 components were identified whose share changed over the vegetative cycle. Antioxidant activity of methanolic extracts of immortelle were determined according to DPPH (IC50 = 23–34 μg/mL) and FRAP (29 μg/mL is equivalent to 1.1‒2.2 mM Fe2+) methods. Acetylcholinesterase inhibitory potential, investigated by modified Ellman’s assay and determined as IC50 values, were 340–440 μg/mL for methanol extracts and 135 μg/mL for essential oil. Metanolic extracts showed strong antioxidant activity and potential to inhibit AChE. Essential oil possesses complex chemical composition, inhibition activity of AChE and weak antioxidant capacity.
{"title":"Helichrysum italicum (Roth) G. Don subsp. italicum from Herzegovina","authors":"S. Talić, I. Odak, Anita Martinović Bevanda, Nikolina Crnjac, M. Paštar","doi":"10.5562/CCA3475","DOIUrl":"https://doi.org/10.5562/CCA3475","url":null,"abstract":"The chemical composition of essential oils isolated from immortelle (Helicrysum italicum subsp. italicum) collected in Herzegovina during five different periods, was investigated by GC/MS analysis. The main compounds were a-pinene (15.7 %) and γ-curcumene (12.8 %), followed by 4,6,9-trimethyldec-8-en-3,5-dione (8.7 %), neryl acetate (6.9 %), limonene (6.4 %) and β-selinene (5.3 %). In total, 69 components were identified whose share changed over the vegetative cycle. Antioxidant activity of methanolic extracts of immortelle were determined according to DPPH (IC50 = 23–34 μg/mL) and FRAP (29 μg/mL is equivalent to 1.1‒2.2 mM Fe2+) methods. Acetylcholinesterase inhibitory potential, investigated by modified Ellman’s assay and determined as IC50 values, were 340–440 μg/mL for methanol extracts and 135 μg/mL for essential oil. Metanolic extracts showed strong antioxidant activity and potential to inhibit AChE. Essential oil possesses complex chemical composition, inhibition activity of AChE and weak antioxidant capacity.","PeriodicalId":10822,"journal":{"name":"Croatica Chemica Acta","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2019-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5562/CCA3475","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43821075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Çiğdem Karaaslan, E. Ince, H. Gurer-Orhan, M. Tavakkoli, O. Firuzi, L. Saso, S. Suzen
Antioxidants are helpful in prevention of several diseases related with oxidative stress including neurodegenerative disorders. In recent studies, carbazoles were given proof of promising antioxidant activities. In this article, 9-ethyl-9H-carbazole hydrazone derivatives were synthesized, characterized and their in vitro antioxidant activity and possible cytotoxic effects were investigated. Furthermore, protective effect of the synthesized derivatives against amyloid β-induced damage in PC12 neuronal cells was examined by using MTT assay. The newly synthesized carbazoles were found to have radical scavenging activity with a varying potency both in cell-free and cell-based in vitro assays. Several compounds, especially such as 3d and 3e, 3m and 3n bearing two halogen groups on the phenyl ring, were found to have cytotoxic activity. However, their cytotoxic activities were not higher than that of melatonin. Several compounds also significantly protected neuronal PC12 cells against amyloid β-induced damage, which can be defined as neuroprotective agents. (4-(2-((9-Ethyl-9H-carbazol-3-yl)methylene)hydrazinyl)benzonitrile) 3r was found as the most active compound with both radical scavenging activity and neuroprotective effects against amyloid β-induced damage. These findings might provide an alternative strategy for developing novel carbazole derivatives for management of neurodegenerative diseases, such as Alzheimer's disease.
{"title":"Behaviour of 9-Ethyl-9H-carbazole Hydrazone Derivatives Against Oxidant Systems","authors":"Çiğdem Karaaslan, E. Ince, H. Gurer-Orhan, M. Tavakkoli, O. Firuzi, L. Saso, S. Suzen","doi":"10.5562/CCA3481","DOIUrl":"https://doi.org/10.5562/CCA3481","url":null,"abstract":"Antioxidants are helpful in prevention of several diseases related with oxidative stress including neurodegenerative disorders. In recent studies, carbazoles were given proof of promising antioxidant activities. In this article, 9-ethyl-9H-carbazole hydrazone derivatives were synthesized, characterized and their in vitro antioxidant activity and possible cytotoxic effects were investigated. Furthermore, protective effect of the synthesized derivatives against amyloid β-induced damage in PC12 neuronal cells was examined by using MTT assay. The newly synthesized carbazoles were found to have radical scavenging activity with a varying potency both in cell-free and cell-based in vitro assays. Several compounds, especially such as 3d and 3e, 3m and 3n bearing two halogen groups on the phenyl ring, were found to have cytotoxic activity. However, their cytotoxic activities were not higher than that of melatonin. Several compounds also significantly protected neuronal PC12 cells against amyloid β-induced damage, which can be defined as neuroprotective agents. (4-(2-((9-Ethyl-9H-carbazol-3-yl)methylene)hydrazinyl)benzonitrile) 3r was found as the most active compound with both radical scavenging activity and neuroprotective effects against amyloid β-induced damage. These findings might provide an alternative strategy for developing novel carbazole derivatives for management of neurodegenerative diseases, such as Alzheimer's disease.","PeriodicalId":10822,"journal":{"name":"Croatica Chemica Acta","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2019-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5562/CCA3481","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41909586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marija Đuroković, D. Matković-Čalogović, I. Janotka
Two Portland cement pastes, CEM I 42.5R and CEM III/A 52.5N were exposed to leaching by soft water throughout a one–year hydration period. Mobility of trace elements was investigated by determination of their pore solution concentration in the course of time. Eleven trace elements were included in this research: antimony, arsenic, cadmium, chromium, cobalt, copper, mercury, nickel, lead, vanadium and zinc. The possible usage of the pore solution trace elements concentration in monitoring of deleterious leaching reactions and prediction of environmental risk was investigated.
两种硅酸盐水泥浆体，CEM I 42.5R和CEM III/A 52.5N，在一年的水化期内暴露于软水浸出。通过测定微量元素在一段时间内的孔溶液浓度来研究微量元素的迁移率。本研究包括11种微量元素：锑、砷、镉、铬、钴、铜、汞、镍、铅、钒和锌。研究了孔隙溶液微量元素浓度在监测有害浸出反应和预测环境风险中的可能用途。
{"title":"Mobility of Trace Elements in Pore Solutions of Portland Cement Pastes Exposed to Leaching","authors":"Marija Đuroković, D. Matković-Čalogović, I. Janotka","doi":"10.5562/CCA3511","DOIUrl":"https://doi.org/10.5562/CCA3511","url":null,"abstract":"Two Portland cement pastes, CEM I 42.5R and CEM III/A 52.5N were exposed to leaching by soft water throughout a one–year hydration period. Mobility of trace elements was investigated by determination of their pore solution concentration in the course of time. Eleven trace elements were included in this research: antimony, arsenic, cadmium, chromium, cobalt, copper, mercury, nickel, lead, vanadium and zinc. The possible usage of the pore solution trace elements concentration in monitoring of deleterious leaching reactions and prediction of environmental risk was investigated.","PeriodicalId":10822,"journal":{"name":"Croatica Chemica Acta","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2019-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5562/CCA3511","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49469088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sakharam B. Dongare, B. Bandgar, Pravin S. Bhale, Sadanand N. Shringare, H. Chavan
A series of 7-azaindolyl hydrazones were prepared by reacting of hydrazides of 7-azaindole-3-acetic acids with aromatic aldehydes and N-substituted indolyl-3-carboxyaldehydes. Structure of all the synthesized compounds were satisfactorily correlated by IR, 1H NMR, 13C NMR and mass spectroscopic evidences. The synthesized compounds were evaluated for their possible anticancer potential against MCF-7 induced breast carcinoma. It is worth mentioning that most of the compounds were considerably active against MCF-7 cell line with GI50 values ranging from 22.3–81.0 μM. The hydrazones of N-1-substituted indole-3-carboxyaldehydes 9f, 9g, 9h, 9c, and 9j were active against MCF-7 cell line with GI50 values less than 40 μM (GI50 = 22.3 and 24.9, 29.6, 30.2 and 37.8 μM respectively) with moderate TGI values (TGI = 56.6, 59.5, 65.5, 70.7 and 94.6 μM respectively). The active compounds were also screened against the normal Vero monkey cell line, which showed moderate selectivity against inhibition of cancer cells.
{"title":"Design, Synthesis, and Spectroscopic Study of 7-Azaindolyl Hydrazones with Anti-Breast Cancer Activity","authors":"Sakharam B. Dongare, B. Bandgar, Pravin S. Bhale, Sadanand N. Shringare, H. Chavan","doi":"10.5562/CCA3418","DOIUrl":"https://doi.org/10.5562/CCA3418","url":null,"abstract":"A series of 7-azaindolyl hydrazones were prepared by reacting of hydrazides of 7-azaindole-3-acetic acids with aromatic aldehydes and N-substituted indolyl-3-carboxyaldehydes. Structure of all the synthesized compounds were satisfactorily correlated by IR, 1H NMR, 13C NMR and mass spectroscopic evidences. The synthesized compounds were evaluated for their possible anticancer potential against MCF-7 induced breast carcinoma. It is worth mentioning that most of the compounds were considerably active against MCF-7 cell line with GI50 values ranging from 22.3–81.0 μM. The hydrazones of N-1-substituted indole-3-carboxyaldehydes 9f, 9g, 9h, 9c, and 9j were active against MCF-7 cell line with GI50 values less than 40 μM (GI50 = 22.3 and 24.9, 29.6, 30.2 and 37.8 μM respectively) with moderate TGI values (TGI = 56.6, 59.5, 65.5, 70.7 and 94.6 μM respectively). The active compounds were also screened against the normal Vero monkey cell line, which showed moderate selectivity against inhibition of cancer cells.","PeriodicalId":10822,"journal":{"name":"Croatica Chemica Acta","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2019-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5562/CCA3418","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45242968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This paper reports on the phytochemical composition as well as antiradical and anticholinesterase potential of volatile oils isolated from Centaurea alba and Centaurea jacea, from Croatia. The volatile components, obtained by hydrodistillation, were determined by GC and GC-MS analyses. A total of 18 compounds were identified in C. alba volatile oil with hexadecanoic acid, germacrene D and tetradecanoic acid as main compounds. A total of 29 compounds were identified in C. jacea volatile oil, with epi-bicyclosesquiphellandrene, aromadendrene and hexadecanoic acid as a major compounds. The tested volatile oils showed low DPPH inhibition potential as well as low to moderate antiAChE potential and low antiBuChE potential.
{"title":"Phytochemical Composition, Antiradical and Anticholinesterase Potentials of Centaurea alba and Centaurea jacea Volatile Oils","authors":"O. Politeo, Ivana Carev, Anita Veljača","doi":"10.5562/CCA3458","DOIUrl":"https://doi.org/10.5562/CCA3458","url":null,"abstract":"This paper reports on the phytochemical composition as well as antiradical and anticholinesterase potential of volatile oils isolated from Centaurea alba and Centaurea jacea, from Croatia. The volatile components, obtained by hydrodistillation, were determined by GC and GC-MS analyses. A total of 18 compounds were identified in C. alba volatile oil with hexadecanoic acid, germacrene D and tetradecanoic acid as main compounds. A total of 29 compounds were identified in C. jacea volatile oil, with epi-bicyclosesquiphellandrene, aromadendrene and hexadecanoic acid as a major compounds. The tested volatile oils showed low DPPH inhibition potential as well as low to moderate antiAChE potential and low antiBuChE potential.","PeriodicalId":10822,"journal":{"name":"Croatica Chemica Acta","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2019-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5562/CCA3458","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42438725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A theoretical analysis of reversible and kinetically controlled electrode reactions in conditions of alternative differential multi-pulse voltammetry (ADMPV) is presented. The degree of reversibility, as well as symmetry of the electron transfer reaction, can be estimated by visual inspection of the ADMP voltammogram. The values of electron transfer coefficient and the standard rate constant of a simple electrode reaction Ox + ne− ⇄ Red, can be determined from the slope of linear dependence of the peak currents ratio on the logarithm of pulse duration. The criteria for recognition of reversible and kinetically controlled electrode reactions by alternative differential multi-pulse voltammetry are given.
{"title":"Simulation of Alternative Differential Multi-pulse Voltammetry. Evaluation of the Electrochemical Reversibility by the Voltammogram Symmetry","authors":"D. Jadreško","doi":"10.5562/CCA3485","DOIUrl":"https://doi.org/10.5562/CCA3485","url":null,"abstract":"A theoretical analysis of reversible and kinetically controlled electrode reactions in conditions of alternative differential multi-pulse voltammetry (ADMPV) is presented. The degree of reversibility, as well as symmetry of the electron transfer reaction, can be estimated by visual inspection of the ADMP voltammogram. The values of electron transfer coefficient and the standard rate constant of a simple electrode reaction Ox + ne− ⇄ Red, can be determined from the slope of linear dependence of the peak currents ratio on the logarithm of pulse duration. The criteria for recognition of reversible and kinetically controlled electrode reactions by alternative differential multi-pulse voltammetry are given.","PeriodicalId":10822,"journal":{"name":"Croatica Chemica Acta","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2019-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5562/CCA3485","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48261975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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