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Quercetin instability in red wines: Insights into precipitation and control approaches 红葡萄酒中槲皮素的不稳定性:沉淀和控制方法的见解
IF 7 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-01 DOI: 10.1016/j.crfs.2026.101319
Alessandra Luciano, Luigi Moio, Angelita Gambuti
One effect of climate change on grape composition is the increased biosynthesis of quercetin and its glycosides in response to UV-B radiation. High concentrations of quercetin in wine can exceed its solubility threshold, resulting in undesirable precipitation that negatively impacts clarity and consumer perception. This poses a challenge to producers in terms of both quality and economics. Starting from a survey of quercetin instability and its presence in wines, this review summarises the current understanding of the biochemical and physicochemical mechanisms that regulate quercetin stability and precipitation in red wines. It also covers the factors involved in quercetin extraction and stability during vinification and oenological strategies to counteract these effects.
Taken together, these findings demonstrate that the stability of quercetin in red wines is governed by a complex interplay of factors, including anthocyanin-mediated copigmentation, pH, temperature, nucleation seeds and the broader phenolic composition of the wine matrix. By elucidating these mechanisms, the review provides a framework for predicting and managing quercetin precipitation, thereby supporting improved wine quality and colloidal stability in the challenging conditions imposed by climate change.
气候变化对葡萄成分的影响之一是槲皮素及其糖苷的生物合成增加,以响应UV-B辐射。葡萄酒中高浓度的槲皮素会超过其溶解度阈值,导致不希望的沉淀,对清晰度和消费者的感知产生负面影响。这对生产者在质量和经济方面都提出了挑战。本文从研究槲皮素的不稳定性及其在葡萄酒中的存在入手,综述了目前对槲皮素在红葡萄酒中稳定性和沉淀的生物化学和物理化学机制的研究进展。它还涵盖了在酿酒过程中涉及槲皮素提取和稳定性的因素以及抵消这些影响的酿酒策略。综上所述,这些发现表明,槲皮素在红酒中的稳定性是由多种因素的复杂相互作用决定的,包括花青素介导的共色素沉着、pH值、温度、成核种子和葡萄酒基质中更广泛的酚类成分。通过阐明这些机制,本综述为预测和管理槲皮素沉淀提供了一个框架,从而支持在气候变化带来的挑战性条件下改善葡萄酒质量和胶体稳定性。
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引用次数: 0
Dendritic crystal pattern from a drying droplet: A distinctive feature of feng-flavor Baijiu 干燥水滴的树枝状晶体图案:枫味白酒的独特特征。
IF 7 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-01 DOI: 10.1016/j.crfs.2026.101324
Haoran Fu , Yang Zhong , Lu Cai , Tieyuan Cheng , Yulin Xia , XiaoHong Jian , Jun Liu , Zaixin Li , Huibo Luo , Ziqing Wu , ChengYong Jin , Yongming Liu , Siqi Yuan , Zhi Zhang
Visual evaluation techniques for Chinese Baijiu remain an active research area. We identified a novel dendritic crystal pattern formed after the droplet evaporation of Feng-flavor Baijiu. Interestingly, this pattern showed an obvious correlation with aging in Jiuhai container (storage vessels for aging) (R2 = 0.904, p < 0.01), suggesting its potential as a visual quality indicator of Feng-flavor Baijiu. During droplet evaporation, the local solution preferentially reaches a supersaturated state at the three-phase contact line, initiating nucleation and crystallization that ultimately assemble into a dendritic crystal pattern with a coffee-ring structure. Further analysis revealed that the crystals are mainly composed of metal ions such as calcium (Ca2+), magnesium (Mg2+), sodium (Na+), and potassium (K+), which primarily originate from a unique aging container known as the Jiuhai container. Furthermore, we observed that trace components such as acids and esters can significantly inhibit or promote the formation of dendritic crystals, contributing to distinct dendritic crystal patterns across various quality grades (Ⅰ to Ⅴ) of Feng-flavor Baijiu samples. More interestingly, crystallization simulation experiments showed that dendritic crystal patterns formed in blended Feng-flavor Baijiu but not in other blended-flavor Baijiu samples. Consequently, the characteristic dendritic crystal patterns generated by droplet evaporation could serve as potential visual indicators for evaluating the quality of Feng-flavor Baijiu.
白酒的视觉评价技术一直是一个活跃的研究领域。我们发现了一种新颖的枫味白酒液滴蒸发后形成的枝晶图案。有趣的是,这一规律与白酒在酒海容器(陈化贮藏容器)中的陈化有明显的相关性(R2 = 0.904,p )。在液滴蒸发过程中,局部溶液在三相接触线上优先达到过饱和状态,开始成核和结晶,最终组装成具有咖啡环结构的枝晶图案。进一步分析表明,这些晶体主要由钙(Ca2+)、镁(Mg2+)、钠(Na+)和钾(K+)等金属离子组成,这些金属离子主要来自一种独特的陈化容器——九海容器。此外,我们还观察到,酸和酯等微量成分可以显著抑制或促进树突晶体的形成,从而导致不同质量等级(Ⅰ至Ⅴ)凤味白酒样品的树突晶体模式不同。更有趣的是,结晶模拟实验表明,枫味混合白酒中形成了树枝状晶体模式,而在其他混合白酒样品中则没有。因此,液滴蒸发过程中产生的树突晶体特征可以作为评价枫香白酒品质的潜在视觉指标。
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引用次数: 0
Structure-effect relationship of phenolic compounds on α-amylase inhibition studied by isothermal titration calorimetry 等温滴定量热法研究酚类化合物抑制α-淀粉酶的构效关系
IF 7 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-01 DOI: 10.1016/j.crfs.2025.101266
Mengyao Xiong , Jörn Plambeck , Tuba Esatbeyoglu , Maria Buchweitz
Recent studies have emphasized the therapeutic potential of polyphenols in regulating blood glucose levels. To assess the inhibitory effects of different phenolic structures and various plant extracts on pancreatic α-amylase in real-time under physiologically relevant conditions, starch hydrolysis was monitored by isothermal titration calorimetry. Significant impact of the phenolic structure was observed for the inhibition strength and mechanism. While some polyphenols demonstrated reversible inhibition, others seem to form aggregates. Traditional apple varieties exhibited greater α-amylase inhibition than commercial varieties. Apple peel, characterized by higher phenolic contents and flavonols, exceeded the inhibition by extracts from flesh. Aronia rich in procyanidins and tea with EGCG revealed excellent and concentration-dependent inhibition. In contrast to cyanidin-3-O-glucoside, black carrot anthocyanins exhibited insignificant inhibition, indicating a limiting impact of the sugar acylation. These findings underscore the role of specific polyphenol structures in modulating carbohydrate digestion, suggesting their potential for managing glycaemic control.
最近的研究强调了多酚在调节血糖水平方面的治疗潜力。为了评估不同酚类结构和不同植物提取物在生理相关条件下对胰腺α-淀粉酶的实时抑制作用,采用等温滴定量热法监测淀粉水解。观察到酚类结构对抑制强度和机理有显著影响。虽然一些多酚表现出可逆的抑制作用,但其他多酚似乎形成聚集体。传统苹果品种表现出比商品品种更强的α-淀粉酶抑制作用。苹果皮具有较高的酚类和黄酮醇含量,超过了果肉提取物的抑制作用。富含原花青素的野樱草和茶对EGCG的抑制效果良好且呈浓度依赖性。与花青素-3- o -葡萄糖苷相比,黑胡萝卜花青素表现出不显著的抑制作用,表明糖酰化的限制作用。这些发现强调了特定多酚结构在调节碳水化合物消化中的作用,表明它们具有控制血糖的潜力。
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引用次数: 0
Molecular sensory science elucidates the contribution of methyl ketones to the creamy aroma of Monascus-fermented cheese 分子感官科学阐明了甲基酮对红曲霉发酵奶酪奶油香气的贡献
IF 7 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-01 DOI: 10.1016/j.crfs.2026.101328
Yadong Wang, Ying Wang, Ting Meng, Bei Wang, Yanbo Wang
Monascus-fermented cheese (MC) is a novel Chinese delicacy characterized by distinctive fruity, creamy, and fermented aromas. This study systematically characterized, using integrated sensory-directed analytical approaches, the aroma compounds during the 90-d ripening stage of MC using molecular sensory science techniques to elucidate the key methyl ketones responsible for its creamy flavor profile. Quantitative descriptive analysis (QDA) revealed a significant increase in the creamy intensity as the ripening process progressed (p < 0.05). Solid-phase microextraction (SPME)-Arrow-gas chromatography-mass spectrometry (GC-MS) identified 115 volatile compounds, of which 36, including 13 methyl ketones, were selected (FD ≥ 81) for further analysis. Further odor activity value (OAV) analysis and aroma omission/addition experiments identified 2-heptanone, 2-nonanone, and 2-undecanone as the key methyl ketone compounds in MC. Stable isotope dilution analysis (SIDA) was employed to quantify these three compounds and to validate their content dynamics across different ripening stages. This study identifies the key compounds behind MC's creamy aroma, providing a scientific basis for future optimization studies.
红曲霉发酵奶酪(MC)是一种新颖的中国美食,其特点是独特的水果味、奶油味和发酵香味。本研究采用综合感官导向分析方法,系统表征了MC成熟90 d期间的香气化合物,并利用分子感官科学技术阐明了其奶油风味的关键甲基酮。定量描述性分析(QDA)显示,随着成熟过程的进行,乳脂强度显著增加(p <; 0.05)。固相微萃取(SPME)-箭头-气相色谱-质谱法(GC-MS)鉴定出115种挥发性化合物,其中36种,包括13种甲基酮(FD ≥ 81)进行进一步分析。进一步的气味活性值(OAV)分析和香气遗漏/添加实验确定了2-庚酮、2-壬烷酮和2-壬烷酮是MC中的关键甲基酮类化合物。采用稳定同位素稀释分析(SIDA)对这三种化合物进行了定量分析,并验证了它们在不同成熟阶段的含量动态。本研究确定了麦香乳脂香气背后的关键化合物,为今后的优化研究提供了科学依据。
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引用次数: 0
Colloidal state and emulsifying properties of protein isolates from kilned and non-kilned oats 熟燕麦和非熟燕麦分离蛋白的胶体状态和乳化特性
IF 7 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-01 DOI: 10.1016/j.crfs.2026.101313
Ines Pynket, Frederik Janssen, Valérie Janssis, Lise Maeseele, Christophe M. Courtin, Arno G.B. Wouters
There is a strong need for basic insights into the colloidal state of oat proteins as function of environmental conditions, and the relationship with functional properties. Relevant in this regard is the commonly applied industrial oat groat heat treatment (‘kilning’) which is here hypothesized to affect the colloidal and functional properties of oat protein isolates (OPIs). Here, we prepared OPIs from non-heat-treated (NHT) and industrially heat-treated (IHT, kilned) oats and investigated colloidal and emulsifying properties of OPINHT and OPIIHT dispersions, as a function of protein concentration (0.5–2.0% wprotein/v) and pH (7.0–9.0). OPINHT and OPIIHT dispersions at pH 7.0 were more prone to aggregation and had lower colloidal stability than at pH 9.0, attributable to increased electrostatic repulsion at higher pH. Notably, initially increasing dispersion pH to 9.0 prior to lowering the pH to 7.0 improved the dispersion colloidal stability at neutral pH, potentially due to irreversible protein structural changes. Overall, OPIIHT dispersions were more colloidally stable than OPINHT dispersions, presumably due to kilning-induced protein structural changes resulting in pronouncedly aggregated proteins in IHT oats not being recovered in the OPI itself. Oil-in-water emulsions prepared from OPIIHT dispersions had higher apparent stability than those prepared from OPINHT dispersions. Moreover, emulsion stability improved (i) at alkaline conditions, where protein aggregation was reduced, and (ii) upon prior protein aggregate removal by centrifugation. These observations suggest a negative influence of oat protein aggregates on emulsion apparent stability; however, the underlying mechanisms remain unclear and should be studied further. These findings offer critical insights into how oat kilning influences the protein colloidal and emulsifying properties.
迫切需要对燕麦蛋白的胶体状态作为环境条件的功能及其与功能特性的关系进行基本的了解。与此相关的是通常应用的工业燕麦热处理(“焙烧”),这里假设它会影响燕麦分离蛋白(OPIs)的胶体和功能特性。本研究以未经热处理(NHT)和工业热处理(IHT)的燕麦为原料制备了opi,并研究了OPINHT和OPIIHT分散体的胶体和乳化性能与蛋白质浓度(0.5-2.0% wprotein/v)和pH(7.0-9.0)的关系。OPINHT和OPIIHT分散体在pH 7.0时比pH 9.0时更容易聚集,胶体稳定性较低,这是由于较高pH下静电斥力的增加。值得注意的是,先将分散体pH提高到9.0,再将pH降低到7.0,在中性pH下分散体的胶体稳定性得到改善,这可能是由于不可逆的蛋白质结构变化。总的来说,OPIIHT分散体比OPINHT分散体在胶体上更稳定,这可能是由于杀戮诱导的蛋白质结构变化导致IHT燕麦中明显聚集的蛋白质在OPI本身中没有被恢复。OPIIHT分散体制备的水包油乳液的表观稳定性优于OPINHT分散体。此外,乳状液稳定性得到改善(i)在碱性条件下,蛋白质聚集减少,(ii)在先前通过离心去除蛋白质聚集后。这些结果表明燕麦蛋白聚集体对乳状液表观稳定性有负面影响;然而,潜在的机制尚不清楚,应进一步研究。这些发现为燕麦烘烤如何影响蛋白质的胶体和乳化特性提供了重要的见解。
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引用次数: 0
Multi-omics reveals microbial community characteristics and flavonoid biotransformation mechanisms during mung bean sour fermentation 多组学揭示了绿豆酸发酵过程中微生物群落特征和类黄酮生物转化机制
IF 7 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-01 DOI: 10.1016/j.crfs.2026.101342
Xunda Wang, Yuxia Yang, Yue Li, Ziqi Zhu, Reham khashaba, Qinghua Yue
Fermented foods are gaining popularity for their health benefits, and fermented mung bean products are recognized for their nutritional value. To investigate microbial community succession and abundance dynamics of bioactive compounds like flavonoids in fermented mung bean sour (MBS), parallel metagenomic and metabolomic analyses were performed. Metagenomic profiling identified Lactiplantibacillus, Lactococcus, Bifidobacterium, and Acetobacter as dominant genera. Functional gene analysis demonstrated a significant increase in the relative abundance of flavonoid degradation pathways, which exhibited strong positive correlations (r > 0.8, p < 0.05) with the dominant genera. Metabolomic analysis revealed a significant decrease in flavonoids, including vitexin and orientin. Moreover, increased activities of β-glucosidase and α-rhamnosidase were identified as key factors promoting the biotransformation of flavonoids into bioactive metabolites such as apigenin, naringenin, and quercetin. This study provides a foundation for further understanding the microbial conversion and utilization of flavonoid compounds in mung beans.
发酵食品因其健康益处而越来越受欢迎,发酵绿豆产品因其营养价值而得到认可。为了研究发酵绿豆酸(MBS)中黄酮类化合物等生物活性物质的微生物群落演替和丰度动态,进行了宏基因组和代谢组学平行分析。宏基因组分析鉴定乳酸菌属、乳球菌属、双歧杆菌属和醋酸杆菌属为优势属。功能基因分析表明,黄酮类降解途径的相对丰度显著增加,与优势属呈强正相关(r >; 0.8,p <; 0.05)。代谢组学分析显示黄酮类化合物含量显著降低,包括牡荆素和荭草苷。此外,β-葡萄糖苷酶和α-鼠李糖苷酶活性的增加是促进黄酮类化合物转化为芹菜素、柚皮素和槲皮素等生物活性代谢物的关键因素。本研究为进一步了解绿豆中黄酮类化合物的微生物转化和利用提供了基础。
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引用次数: 0
Integrated hyperspectral–RGB modeling for the estimation of wheat protein and dough rheological properties using machine and deep transfer learning 基于机器和深度迁移学习的小麦蛋白质和面团流变特性综合高光谱- rgb建模。
IF 7 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-01 DOI: 10.1016/j.crfs.2026.101329
Shaohua Zhang , Qianya Cheng , Tiantian Wang , Changjun Dai , Jichun Tian , Wei Feng , Chenyang Wang , Tiancai Guo , Li He
The rapid non-destructive estimation of wheat protein properties and dough rheological characteristics is of great significance for ensuring processing quality, food safety, and variety classification. This study established a multi-source remote sensing framework integrating hyperspectral and red–green–blue (RGB) data. By extracting wavelet features (WFs) and color indices (CIs), and applying Pearson correlation analysis combined with the successive projections algorithm–variance inflation factor (SPA-VIF) for feature selection, partial least squares regression (PLSR), extreme gradient boosting (XGBoost), and attention-based multi-task learning (MTL-AM) were employed to predict wheat protein and rheological traits. The results showed that the fusion of wavelet features with RGB images significantly improved model prediction accuracy, and that the estimation performance of XGBoost and MTL-AM was significantly higher than that of PLSR. To further improve the estimation accuracy of the farinograph quality number (FQN), the combined MTL-AM–XGBoost model achieved the highest accuracy (R2 = 0.982, RMSE = 8.975, MAE = 7.087, RPD = 6.077). This study provides an effective technical solution for the non-destructive, high-throughput monitoring of wheat functional quality.
小麦蛋白特性和面团流变特性的快速无损检测对保证加工质量、食品安全和品种分类具有重要意义。本研究建立了高光谱与红绿蓝(RGB)数据相结合的多源遥感框架。通过提取小波特征(WFs)和颜色指数(CIs),采用Pearson相关分析结合逐次预测算法-方差膨胀因子(SPA-VIF)进行特征选择,采用偏最小二乘回归(PLSR)、极端梯度增强(XGBoost)和基于注意的多任务学习(MTL-AM)预测小麦蛋白质和流变性状。结果表明,小波特征与RGB图像的融合显著提高了模型的预测精度,XGBoost和MTL-AM的估计性能明显高于PLSR。为进一步提高粉质图质量数(FQN)的估计精度,MTL-AM-XGBoost联合模型的准确率最高(R2 = 0.982,RMSE = 8.975,MAE = 7.087,RPD = 6.077)。本研究为小麦功能品质的无损、高通量监测提供了有效的技术解决方案。
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引用次数: 0
Nutritional quality indicators as complementary functional units for the sustainability assessment of grain-based foods in Sweden 营养质量指标作为瑞典谷物食品可持续性评估的补充功能单位
IF 7 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-01 DOI: 10.1016/j.crfs.2026.101310
Martina Cassarino , Gianluca Giuberti , Margherita Dall’Asta , Hanieh Moshtaghian , Marta Bianchi
Grain-based products, particularly whole grains, are integral to a healthy and sustainable diet. However, their nutritional quality and environmental impact vary, necessitating consideration of both aspects in sustainability assessments. This study aimed to identify nutritional indicators for grain-based foods and evaluate their suitability as functional units for carbon footprint analysis of Swedish products. Six indicators—CFQS-5, Keyhole, NRF11.3, NR-FIcarb, Nutri-Score, and Nutri-Scorewholegrain—were applied to 356 foods. Nutri-Score classified the highest proportion of products as high-quality (40 %), while Keyhole classified the fewest (13 %). Correlation with wholegrain content was strongest for CFQS-5 and weakest for Nutri-Scorewholegrain. NRF11.3 and NR-FIcarb emerged as the most suitable functional units for carbon footprint calculations, showing moderate, statistically significant positive correlations with wholegrain content (ρ = 0.452 for NRF11.3, ρ = 0.475 for NR-FIcarb, both p < 0.001) and effectively rating grain-food categories in line with dietary guidelines. Transitioning from a weight-based to a nutrition-based functional unit, expressed as either NRF11.3 or NR-FIcarb, did not alter the finding that rice and sweet bakery products had the highest greenhouse gas emissions. However, it highlighted the favorable nutritional quality of “hard bread and crusts” and “flour, cereals, grains, and crushed cereals,” leading to reduced carbon footprints. These findings demonstrate that NRF11.3 and NR-FIcarb are equally effective in assessing the quality of grain-based foods and can be applied in food life cycle assessments, providing a practical tool for identifying foods with lower climate impact and higher nutritional value.
以谷物为基础的产品,特别是全谷物,是健康和可持续饮食的组成部分。然而,它们的营养质量和环境影响各不相同,因此需要在可持续性评估中考虑这两个方面。本研究旨在确定谷物食品的营养指标,并评估其作为瑞典产品碳足迹分析功能单位的适用性。采用cfqs -5、Keyhole、NRF11.3、NR-FIcarb、nutrition - score和nutrition - scorewhole - grain 6项指标对356种食品进行评价。Nutri-Score将产品评为优质的比例最高(40 %),而Keyhole将产品评为优质的比例最低(13 %)。与全谷物含量相关性最强的是CFQS-5,与全谷物营养评分相关性最弱。NRF11.3和NR-FIcarb是计算碳足迹的最合适的功能单位,它们与全谷物含量(NRF11.3的ρ = 0.452,NR-FIcarb的ρ = 0.475,p均为 <; 0.001)呈正相关,并有效地根据膳食指南对谷物食品类别进行评级。从以体重为基础的功能单位转变为以营养为基础的功能单位,用NRF11.3或NR-FIcarb表示,并没有改变大米和甜烘焙产品温室气体排放量最高的发现。然而,它强调了“硬面包和硬皮”和“面粉、谷物、谷物和碎谷物”的良好营养品质,从而减少了碳足迹。这些发现表明,NRF11.3和NR-FIcarb在评估谷物食品质量方面同样有效,可应用于食品生命周期评估,为识别气候影响较小、营养价值较高的食品提供实用工具。
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引用次数: 0
Development of a synbiotic dietary supplement containing potential Next Generation Probiotics for modulation of the gut microbiome and metabolome 一种含有潜在的下一代益生菌的合成膳食补充剂的开发,用于调节肠道微生物群和代谢组
IF 7 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-01 DOI: 10.1016/j.crfs.2025.101289
Alessia Esposito , Vincenzo Valentino , Silvia Tagliamonte , Giuseppina Sequino , Paola Vitaglione , Danilo Ercolini , Francesca De Filippis
The term Next Generation Probiotics (NGPs) refers to microbial strains positively impacting on human health, but do not belong to common probiotic species (e.g., lactic acid bacteria, LAB). We characterized genomically and phenotypically 14 strains isolated from the gut microbiome of healthy individuals, to evaluate their ability to produce urolithins, equol and short-chain fatty acids (SCFA). The 4 most promising strains (namely Bacteroides uniformis A4, Bacteroides thetaiotaomicron A14, unclassified Bacteroidaceae A26 and unclassified Lachnospiraceae A49) were used for the production of a synbiotic formulation, containing the strains and the precursors of health-promoting molecules. This dietary supplement was administered for 2 weeks to a continuous mucosal-Simulator of the Human Intestinal Microbial Ecosystem (mSHIME) model inoculated with a faecal sample from a low fiber-consuming donor. We performed Shotgun Metagenome Sequencing on a total of 204 samples collected from lumen and mucosa compartments, and determined the concentration of SCFA, equol and urolithin. Our results highlighted that the potential NGP strains contained in the supplement persisted in the gut ecosystem during 2 weeks of washout (Wilcoxon's rank sum test, p-value <0.05). In addition, the treatment led to an enrichment in beneficial taxa and to an increase in the production of SCFAs (p-value <0.05). This study demonstrated that feeding the gut microbiota with NGPs and dietary prebiotics can modulate both the gut microbiome and metabolome, suggesting a potential beneficial impact on human health. However, further in vivo studies are needed to confirm these results.
下一代益生菌(NGPs)是指对人类健康有积极影响的微生物菌株,但不属于常见的益生菌物种(如乳酸菌,LAB)。我们对从健康个体的肠道微生物组中分离的14株菌株进行了基因组和表型表征,以评估它们产生尿石素、马酚和短链脂肪酸(SCFA)的能力。利用4种最有希望的菌株(即拟杆菌A4、拟杆菌A14、未分类拟杆菌科A26和未分类毛螺科A49)制备了含有这些菌株和促健康分子前体的合成制剂。该膳食补充剂被给予连续的粘膜模拟器人类肠道微生物生态系统(mSHIME)模型,该模型接种了来自低纤维消耗供者的粪便样本。我们对从管腔和粘膜室收集的204个样本进行了Shotgun宏基因组测序,并测定了SCFA、雌马酚和尿素的浓度。我们的研究结果强调,在洗脱试验的2周内,补充剂中含有的潜在NGP菌株在肠道生态系统中持续存在(Wilcoxon秩和检验,p值<;0.05)。此外,处理导致有益类群的丰富和scfa产量的增加(p值<;0.05)。该研究表明,用ngp和膳食益生元喂养肠道微生物群可以调节肠道微生物群和代谢组,表明对人类健康有潜在的有益影响。然而,需要进一步的体内研究来证实这些结果。
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引用次数: 0
Cooling sensation biosensor coupled with the Chou-Talalay method for quantitative assessment of interaction patterns in binary cooling agent mixtures 冷感生物传感器耦合Chou-Talalay方法定量评价二元冷却剂混合物中相互作用模式
IF 7 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-01 DOI: 10.1016/j.crfs.2025.101287
Bo Wan, Quanping Yan, Fuwei Xie, Chenfeng Hua, Cong Nie, Xuejie Li, Pingping Shang, Xiaobing Zhang, Hongbo Wang, Bin Peng, Chengjie Ma, Ge Zhao
Modulating cooling sensation is crucial for enhancing consumer appeal in food and daily chemical products. Conventional methods struggle to quantify cooling sensation and its synergistic effects, especially in mimicking human perception. Here, we developed a highly sensitive cell-based biosensor for in vitro cooling sensation assessment. This biosensor successfully quantified menthol, WS-3, and WS-23. Notably, the Chou-Talalay method was integrated with the biosensor to quantitatively investigate synergistic and antagonistic effects among these cooling agents. Our strategy revealed that interactions were highly dependent on both combinations and ratios; for instance, menthol/WS-3 (1:3) showed pronounced synergism, while certain WS-3/WS-23 ratios (1:3) exhibited antagonism. Sensory evaluation strongly correlated with biosensor measurements, confirming the high predictive validity of our method. This biomimetic strategy establishes a new paradigm for physiologically relevant cooling sensation analysis. Moreover, this integrated biosensor and the Chou-Talalay method also holds significant potential for exploring other taste analyses.
调节冷却感觉对提高食品和日化产品的消费者吸引力至关重要。传统的方法难以量化冷却感觉及其协同效应,特别是在模仿人类感知方面。在这里,我们开发了一种高灵敏度的基于细胞的生物传感器,用于体外冷却感觉评估。这种生物传感器成功地定量了薄荷醇、WS-3和WS-23。值得注意的是,Chou-Talalay方法与生物传感器相结合,定量研究了这些冷却剂之间的协同和拮抗作用。我们的策略显示,相互作用高度依赖于组合和比率;例如,薄荷醇/WS-3(1:3)表现出明显的协同作用,而某些WS-3/WS-23比率(1:3)表现出拮抗作用。感官评估与生物传感器测量密切相关,证实了我们方法的高预测有效性。这种仿生策略为生理相关的冷感觉分析建立了新的范式。此外,这种集成的生物传感器和Chou-Talalay方法也具有探索其他味道分析的巨大潜力。
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Current Research in Food Science
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