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Extraction-depended and thermally-modulated physical and chemical properties of powders produced from cranberry pomace extracts 蔓越莓渣提取物粉末的提取依赖性和热调节物理化学特性
IF 6.3 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2023.100664
Jessica Brzezowska, Aleksandra Hendrysiak, Aneta Wojdyło, Anna Michalska-Ciechanowska

Recovering bioactives from botanical by-products in the form of powders has been attempted through a number of multidirectional approaches. Yet understanding the processing of such plant formulations requires dedicated research owing to the manifold factors shaping the quality of powders. Therefore, the study aimed at production of cranberry powders from pomace extracts and to evaluate how different solvent type, carriers and drying techniques modulate their physico-chemical properties. Freeze- and vacuum drying significantly differentiated samples in terms of physical properties, while the extraction solvent and carrier type had substantial impact on chemical ones. For carrier-added products pomace extraction with acidified 50% ethanol resulted in the highest content of identified phenolics in powders (up to 5.87 g · 100 g−1 dry matter), while 30% acetone in the lowest (on average, 3.94 g · 100 g−1 dry matter). Acetone extraction strengthened the formation of hydroxymethyl-L-furfural that was higher when compared to acidified 50% ethanol, while trace amounts were reported for non-acidified counterpart. Similar observation was made in the case of flavan-3-ols. Addition of carriers during powders production led to the lower hydroxymethyl-L-furfural formation even down to 74% with regard to carrier-free samples. The study confirmed feasibility of managing cranberry pomace into high-value powders in extraction-depended and thermally-modulated quality matter.

人们尝试通过多种多向方法从粉末形式的植物副产品中回收生物活性物质。然而,由于影响粉末质量的因素是多方面的,因此了解此类植物制剂的加工过程需要进行专门的研究。因此,本研究旨在从果渣提取物中生产蔓越莓粉末,并评估不同溶剂类型、载体和干燥技术如何改变其物理化学特性。冷冻干燥和真空干燥对样品的物理性质有显著差异,而提取溶剂和载体类型对化学性质有很大影响。对于添加载体的产品,用酸化的 50%乙醇萃取果渣,粉末中的酚含量最高(达 5.87 克-100 克-1 干物质),而用 30% 丙酮萃取,酚含量最低(平均 3.94 克-100 克-1 干物质)。丙酮萃取加强了羟甲基-L-糠醛的形成,与酸化的 50%乙醇相比,羟甲基-L-糠醛的含量更高,而非酸化的乙醇则只有微量的羟甲基-L-糠醛。黄烷-3-醇也有类似的观察结果。在粉末生产过程中添加载体会降低羟甲基-L-糠醛的形成,甚至比不添加载体的样品低 74%。这项研究证实了将蔓越莓渣加工成高价值粉末的可行性,其质量取决于萃取和热调节。
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引用次数: 0
Characterizations of emulsion gel formed with the mixture of whey and soy protein and its protein digestion under in vitro gastric conditions 乳清蛋白和大豆蛋白混合物形成的乳状凝胶及其在体外胃条件下的蛋白质消化特性
IF 6.3 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2023.100674
Yu Cheng , Aiqian Ye , Harjinder Singh

Partially replacing animal proteins with plant proteins to develop new products has much attention. To get knowledge of their application in emulsion gels, heat-induced composite protein emulsion gels were fabricated using the mixtures of whey protein isolate (WPI) and soy protein isolate (SPI) with the final total protein concentration of 10% (w/w). The water holding capacity (WHC), mechanical and rheological properties and microstructure of mixed protein emulsion gels prepared at different WPI to SPI ratios (100:0, 90:10, 70:30, 50:50, 30:70, 10:90, 0:100, w/w) were investigated. The ratios of WPI to SPI showed little effect on the WHC of the mixed protein emulsion gels (p > 0.05). Increasing the ratio of SPI decreased the hardness and storage modulus (G′) of mixed protein emulsion gels, whereas the porosity of mixed protein emulsion gels in the microstructure increased, as shown by CLSM. Both β-lactoglobulin and α-lactalbumin from WPI and 7 S and 11 S from SPI participated in forming the gel matrix of mixed protein emulsion gels. More protein aggregates existed as the gel matrix filler at the high soy protein levels. Interestingly, the G′ of mixed protein emulsion gels at the WPI to SPI ratio of 50:50 was higher than the sum of G′ of individual WPI and SPI emulsion gels. The whey protein network predominated the gel matrix, while soy protein predominated in the active filling effect. When subjected to an in vitro dynamic gastric digestion model, soy protein in the gels (WPI:SPI = 50:50) degraded faster than whey protein during gastric digestion. This study provided new information on the characteristics of composite protein emulsion gel fabricated with the WPI and SPI mixture.

用植物蛋白部分替代动物蛋白以开发新产品的做法备受关注。为了了解植物蛋白在乳液凝胶中的应用,研究人员使用乳清蛋白分离物(WPI)和大豆蛋白分离物(SPI)的混合物(最终总蛋白浓度为 10%(w/w))制作了热诱导复合蛋白乳液凝胶。研究了以不同的 WPI 与 SPI 比例(100:0、90:10、70:30、50:50、30:70、10:90、0:100,w/w)制备的混合蛋白乳液凝胶的持水量(WHC)、机械和流变特性以及微观结构。WPI 与 SPI 的比例对混合蛋白乳液凝胶的 WHC 影响不大(p > 0.05)。增加 SPI 的比例会降低混合蛋白乳液凝胶的硬度和储存模量(G′),而混合蛋白乳液凝胶微观结构中的孔隙率则会增加,如 CLSM 所示。WPI 中的β-乳球蛋白和α-乳白蛋白以及 SPI 中的 7 S 和 11 S 都参与了混合蛋白乳液凝胶凝胶基质的形成。大豆蛋白含量高时,凝胶基质填充物中存在更多的蛋白质聚集体。有趣的是,当 WPI 与 SPI 的比例为 50:50 时,混合蛋白乳液凝胶的 G′高于单个 WPI 和 SPI 乳液凝胶的 G′之和。乳清蛋白网络在凝胶基质中占主导地位,而大豆蛋白在活性填充效应中占主导地位。在体外动态胃消化模型中,凝胶(WPI:SPI = 50:50)中的大豆蛋白在胃消化过程中的降解速度快于乳清蛋白。这项研究为了解用 WPI 和 SPI 混合物制成的复合蛋白质乳液凝胶的特性提供了新的信息。
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引用次数: 0
Prevalence, antibiotic resistance and molecular characterization of Staphylococcus aureus in ready-to-eat fruits and vegetables in Shanghai, China 中国上海即食果蔬中金黄色葡萄球菌的流行率、抗生素耐药性和分子特征
IF 6.3 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2023.100669
Kai Jia , Xiaojie Qin , Xiangfeng Bu, Huajian Zhu, Yangtai Liu, Xiang Wang, Zhuosi Li, Qingli Dong

Staphylococcus aureus (S. aureus) is one of the foodborne pathogens. This study aimed to investigate the prevalence of S. aureus in ready-to-eat (RTE) fruits and vegetables in Shanghai, China. We evaluated antibiotic resistance patterns and genetic diversity of isolates through whole genome sequencing. Our findings demonstrated that out of 143 market samples, 47 (32.87%) tested positive for S. aureus, with the prevalence rates ranging from 10% to 57.14% among 12 types of RTE fruits and vegetables. Most isolates were resistant to trimethoprim-sulphamethoxazole, oxacillin, and ampicillin. We identified a total of 15 antibiotic resistance genes associated with resistance to 6 antibiotics, such as fosfomycin, fluoroquinolone, and β-lactam. Adhesion genes and enterotoxin genes, including icaA, icaB, icaC, set, seg, and sec, were also identified. Seven multi-locus sequence types (MLST) were detected, two of which were novel (ST7208 and ST7986). Notably, ST705-t529 (34.04%) and ST6-t701 (27.79%) represented the predominant types of S. aureus. Furthermore, three of the isolates were confirmed to be methicillin-resistant S. aureus by mecA genes. Taken together, our results highlight the high prevalence of S. aureus in RTE fruits and vegetables, posing a potential threat to food safety, particularly due to its high level of antibiotic resistance.

金黄色葡萄球菌(S. aureus)是食源性致病菌之一。本研究旨在调查金黄色葡萄球菌在中国上海即食果蔬中的流行情况。我们通过全基因组测序评估了分离菌株的抗生素耐药性模式和遗传多样性。我们的研究结果表明,在 143 份市场样品中,有 47 份(32.87%)的金黄色葡萄球菌检测结果呈阳性,在 12 种即食果蔬中的流行率从 10% 到 57.14% 不等。大部分分离菌株对三甲氧苄青霉素-磺胺甲噁唑、氧西林和氨苄西林有抗药性。我们共发现了 15 个抗生素耐药基因,这些基因与对 6 种抗生素(如磷霉素、氟喹诺酮类和β-内酰胺类)的耐药性有关。此外,还发现了黏附基因和肠毒素基因,包括 icaA、icaB、icaC、set、seg 和 sec。检测到七种多焦点序列类型(MLST),其中两种是新型的(ST7208 和 ST7986)。值得注意的是,ST705-t529(34.04%)和 ST6-t701(27.79%)是金黄色葡萄球菌的主要类型。此外,其中 3 个分离株通过 mecA 基因证实为耐甲氧西林金黄色葡萄球菌。综上所述,我们的研究结果凸显了金黄色葡萄球菌在即食水果和蔬菜中的高流行率,特别是由于其高度的抗生素耐药性,对食品安全构成了潜在威胁。
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引用次数: 0
Characterization, mathematical modeling of moisture sorption isotherms and bioactive compounds of Andean root flours 安第斯根粉的特性、水分吸附等温线数学模型和生物活性化合物
IF 6.3 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2024.100752
Liliana Acurio , Diego Salazar , María Eugenia García , Purificación García-Segovia , Javier Martínez-Monzó , Marta Igual

Andean roots can be used as an alternative to gluten-free food. The objective of this study was to enhance the technological and nutritional properties of Andean root flours to promote their industrial applicability. The water content and activity of the flour were lower than those required to prevent mold growth. The bulk density of the flour was comparable to that of wheat flour. The flour of Ipomoea batatas (L.) Lam. exhibited the lowest water absorption capacity of the tested samples. However, both this flour and Tropaeolum tuberosum Ruiz & Pavón showed a higher fat absorption capacity. The samples exhibited type-II isotherms, indicating that the flours were highly hygroscopic. The Guggenheim, Anderson, and de Boer GAB model showed a higher coefficient of determination in mathematical modeling. The chroma of T. tuberosum Ruiz & Pavón flour was higher than the other samples, which was related to total carotenoids and lycopene. Furthermore, I. batatas (L.) Lam. exhibited the highest phenol value.

安第斯根可以作为无麸质食品的替代品。这项研究的目的是提高安第斯根面粉的技术和营养特性,以促进其工业应用。面粉的含水量和活性均低于防止霉菌生长的要求。面粉的体积密度与小麦粉相当。在测试的样品中,Ipomoea batatas (L.) Lam.面粉的吸水能力最低。然而,这种面粉和 Tropaeolum tuberosum Ruiz & Pavón 都显示出较高的脂肪吸收能力。样品表现出 II 型等温线,表明面粉具有很强的吸湿性。古根海姆、安德森和德布尔 GAB 模型在数学建模中显示出较高的确定系数。T. tuberosum Ruiz & Pavón面粉的色度高于其他样品,这与总类胡萝卜素和番茄红素有关。此外,I. batatas (L.) Lam.的酚值最高。
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引用次数: 0
Enzymatic degradation of pea fibers changes pea protein concentrate functionality 豌豆纤维的酶降解改变了豌豆浓缩蛋白的功能
IF 6.3 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2024.100744
Joël I. Zink , Olivia Zehnder-Wyss , Dylan Dällenbach , Laura Nyström , Erich J. Windhab

Pea proteins are gaining increased interest from both the food industry as well as from consumers. Pea protein isolates (PPI) excel at forming meat-like textures upon heating while pea protein concentrates (PPC) are more challenging to transform into highly sought-after foods. PPCs are richer in dietary fibers (DF) and are more sustainable to produce than PPI. In this work, degradative enzymes were used to modify the functionality of PPC-water blends with a focus on texturization upon heating. Three enzyme solutions containing β-glucanases, hemicellulases, pectinases, xylanase, and cellulases were added to 65 wt% PPC blends. The effect of these enzymatic pretreatments was measured by monitoring the torque in a mixing reactor during blending, differential scanning calorimetry (DSC), high-pressure shear rheology (HPSR), and DF content and size analysis. Four endothermic peaks were detected in the DSC thermograms of PPC, namely at 63 °C, 77 °C, 105 °C and 123 °C. The first three peaks were attributed to phase transition and gelation temperatures of the starches and proteins constituting PPC. No endothermic peaks were measured for PPI blends. Enzyme solutions containing β-glucanases, hemicellulases, pectinases, and xylanases increased the endothermic energy of all peaks, hinting at an effect on the gelation properties of PPC. The same enzymes decreased the resistance to flow of PPC blends and induced a shift of the weight average molecular weight (Mw) distribution of soluble dietary fibers (SDF) towards smaller values while increasing the fraction of SDF by decreasing the insoluble dietary fiber (IDF) content. The solution containing cellulases did not change the DSC results or the viscosity of the PPC mixture, nor did it affect the IDF and SDF contents. On the other hand HPSR measurements of heated PPC samples up to 125 °C showed that all tested enzyme solutions decreased the complex viscosity of PPC-water blends to values similar to PPI-water blends. We demonstrated that degradative enzymes can enhance the functionality of less refined protein-rich ingredients based on pea and other vegetal sources. Using optimized enzyme blends for targeted applications can prove to be a key changer in the development and improvement of sustainable protein-rich foods.

豌豆蛋白越来越受到食品行业和消费者的关注。豌豆蛋白分离物(PPI)擅长在加热后形成类似肉类的口感,而豌豆蛋白浓缩物(PPC)则更难转化为广受欢迎的食品。浓缩豌豆蛋白含有更丰富的膳食纤维(DF),与浓缩豌豆蛋白相比,其生产更具可持续性。在这项工作中,降解酶被用于改变 PPC-水混合物的功能,重点是加热后的质构化。65 wt% 的 PPC 混合物中添加了三种酶溶液,分别含有 β-葡聚糖酶、半纤维素酶、果胶酶、木聚糖酶和纤维素酶。通过监测混合过程中混合反应器中的扭矩、差示扫描量热法(DSC)、高压剪切流变学(HPSR)以及 DF 含量和粒度分析,测量了这些酶预处理的效果。在 PPC 的 DSC 热图中检测到四个内热峰,分别位于 63 ℃、77 ℃、105 ℃ 和 123 ℃。前三个峰归因于构成 PPC 的淀粉和蛋白质的相变和凝胶化温度。在 PPI 混合物中没有测得内热峰。含有 β-葡聚糖酶、半纤维素酶、果胶酶和木聚糖酶的酶溶液增加了所有峰值的内热能量,暗示其对 PPC 的凝胶特性有影响。同样的酶降低了 PPC 混合物的流动阻力,并使可溶性膳食纤维(SDF)的重量平均分子量(Mw)分布向较小值移动,同时通过降低不溶性膳食纤维(IDF)的含量来增加 SDF 的比例。含有纤维素酶的溶液不会改变 DSC 结果或 PPC 混合物的粘度,也不会影响 IDF 和 SDF 的含量。另一方面,对加热至 125 °C 的 PPC 样品进行的 HPSR 测量显示,所有测试的酶溶液都降低了 PPC-水混合物的复合粘度,使其达到与 PPI-水混合物相似的值。我们证明,降解酶可以提高基于豌豆和其他植物来源的不太精制的富含蛋白质成分的功能。将优化的酶混合物用于目标应用可证明是开发和改进可持续富含蛋白质食品的关键变革。
{"title":"Enzymatic degradation of pea fibers changes pea protein concentrate functionality","authors":"Joël I. Zink ,&nbsp;Olivia Zehnder-Wyss ,&nbsp;Dylan Dällenbach ,&nbsp;Laura Nyström ,&nbsp;Erich J. Windhab","doi":"10.1016/j.crfs.2024.100744","DOIUrl":"https://doi.org/10.1016/j.crfs.2024.100744","url":null,"abstract":"<div><p>Pea proteins are gaining increased interest from both the food industry as well as from consumers. Pea protein isolates (PPI) excel at forming meat-like textures upon heating while pea protein concentrates (PPC) are more challenging to transform into highly sought-after foods. PPCs are richer in dietary fibers (DF) and are more sustainable to produce than PPI. In this work, degradative enzymes were used to modify the functionality of PPC-water blends with a focus on texturization upon heating. Three enzyme solutions containing <em>β</em>-glucanases, hemicellulases, pectinases, xylanase, and cellulases were added to 65 wt% PPC blends. The effect of these enzymatic pretreatments was measured by monitoring the torque in a mixing reactor during blending, differential scanning calorimetry (DSC), high-pressure shear rheology (HPSR), and DF content and size analysis. Four endothermic peaks were detected in the DSC thermograms of PPC, namely at 63 °C, 77 °C, 105 °C and 123 °C. The first three peaks were attributed to phase transition and gelation temperatures of the starches and proteins constituting PPC. No endothermic peaks were measured for PPI blends. Enzyme solutions containing <em>β</em>-glucanases, hemicellulases, pectinases, and xylanases increased the endothermic energy of all peaks, hinting at an effect on the gelation properties of PPC. The same enzymes decreased the resistance to flow of PPC blends and induced a shift of the weight average molecular weight (Mw) distribution of soluble dietary fibers (SDF) towards smaller values while increasing the fraction of SDF by decreasing the insoluble dietary fiber (IDF) content. The solution containing cellulases did not change the DSC results or the viscosity of the PPC mixture, nor did it affect the IDF and SDF contents. On the other hand HPSR measurements of heated PPC samples up to 125 °C showed that all tested enzyme solutions decreased the complex viscosity of PPC-water blends to values similar to PPI-water blends. We demonstrated that degradative enzymes can enhance the functionality of less refined protein-rich ingredients based on pea and other vegetal sources. Using optimized enzyme blends for targeted applications can prove to be a key changer in the development and improvement of sustainable protein-rich foods.</p></div>","PeriodicalId":10939,"journal":{"name":"Current Research in Food Science","volume":"8 ","pages":"Article 100744"},"PeriodicalIF":6.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2665927124000704/pdfft?md5=fea2d9215af2fcdc10cdf520d5c84985&pid=1-s2.0-S2665927124000704-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140948636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sporolactobacillus—a new functional genus with potential applications 孢子乳杆菌--具有潜在应用价值的新功能菌属
IF 6.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2024.100822
Xinyu Guo , Leilei Yu , Meifang Xiao , Xiaojie Zang , Chengcheng Zhang , Arjan Narbad , Wei Chen , Fengwei Tian , Qixiao Zhai

Sporolactobacillus is a genus of lactic acid bacteria, which can be widely found in soil. According to NCBI, only 20 strains of the genus Sporolactobacillus have been identified through phenotypic and genotypic analysis, indicating their relatively low numbers compared to other lactic acid bacteria. Currently, there is a growing interest in isolating and studying Sporolactobacillus, particularly focusing on its physiological characteristics and conducting in vitro experiments. This paper provides a review of the sources and physiological characteristics of Sporolactobacillus, along with genotype analysis, carbohydrate metabolism traits, and potential antibacterial properties. It also delves into basic physiological characteristics, lactic acid production, and applications, offering insights for the future utilization of Sporolactobacillus and laying a foundation for exploring its potential applications.

孢子乳酸菌是一种乳酸菌属,广泛存在于土壤中。根据 NCBI 的资料,通过表型和基因型分析,孢子乳酸菌属只有 20 株菌株被鉴定出来,这表明与其他乳酸菌相比,孢子乳酸菌属的数量相对较少。目前,人们对分离和研究孢子乳杆菌的兴趣日益浓厚,尤其关注其生理特征和体外实验。本文综述了孢子乳杆菌的来源和生理特征,以及基因型分析、碳水化合物代谢特征和潜在的抗菌特性。本文还深入探讨了孢子乳杆菌的基本生理特征、乳酸生产和应用,为今后利用孢子乳杆菌提供了启示,并为探索其潜在应用奠定了基础。
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引用次数: 0
Chemical modification of bacterial exopolysaccharides: Antioxidant properties and health potentials 细菌外多糖的化学修饰:抗氧化特性和保健潜力
IF 6.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2024.100824
Irshad Ahmad Shah , Digambar Kavitake , Swati Tiwari , Palanisamy Bruntha Devi , G. Bhanuprakash Reddy , Krishna Kumar Jaiswal , Amit K. Jaiswal , Prathapkumar Halady Shetty

In recent years, there has been a burgeoning interest in the utilization of microbial exopolysaccharides (EPS) because of the added advantage of their renewable, biocompatible, and biodegradable nature in addition to intended applications. The endowed properties of bacterial EPS make them valuable candidates for a wide array of industrial applications. Modification of native EPS is known to enhance various physico-chemical and functional properties. Various modifications such as physical, chemical, biological, and enzymatic modifications were practiced improving the bioactivity of EPS. This paper comprehensively aims to review the most recent chemical modification techniques employed to modify the physico-chemical and functional changes of bacterial EPS in comparison with the unmodified forms. Chemical modification entails strategic alterations to the structure and properties of EPS through various synthetic and semi-synthetic methodologies. Emphasis is given to the antioxidant potential and functional role of these EPS derivatives in human health. Antioxidant properties reveal a significant augmentation in activity compared to their native counterparts. Such enhancement holds a strong promise for potential benefits and therapeutic applications. Chemical derivatives of EPS with overwhelming functional benefits could surely encourage EPS application, particularly as potential hydrocolloids in industrial and biomedical contexts.

近年来,人们对利用微生物外多糖(EPS)的兴趣日益浓厚,因为除了预期的用途外,它们还具有可再生、生物相容性和可生物降解的额外优势。细菌 EPS 所具有的特性使其成为广泛工业应用的宝贵候选物质。众所周知,对原生发泡聚苯乙烯进行改性可增强其各种物理化学和功能特性。各种改性,如物理、化学、生物和酶改性,都能提高 EPS 的生物活性。本文旨在全面回顾最新的化学修饰技术,与未经修饰的形式相比,这些技术可改变细菌 EPS 的物理化学和功能变化。化学修饰需要通过各种合成和半合成方法对 EPS 的结构和特性进行战略性改变。重点是这些 EPS 衍生物在人类健康中的抗氧化潜力和功能作用。与原生衍生物相比,这些衍生物的抗氧化性显著增强。这种增强为潜在的益处和治疗应用带来了巨大希望。EPS 化学衍生物具有巨大的功能优势,必将促进 EPS 的应用,特别是作为潜在的水胶体在工业和生物医学领域的应用。
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引用次数: 0
Steam explosion pretreatment enhances free/combined phytosterol extraction and utilization in rapeseed (Brassica napus L.) and its processed products: Insights from SPE-GC approach 蒸汽爆破预处理可提高油菜籽(Brassica napus L.)及其加工产品中游离/复合植物甾醇的提取和利用:SPE-GC 方法的启示
IF 6.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2024.100869
Dan Wang , Dong Li , Qiuhui Xu , Xin Lv , Hong Chen , Fang Wei
The study investigates the impact of steam explosion pretreatment on the distribution of free and combined phytosterols within rapeseed and its derived products. Utilizing solid phase extraction-gas chromatography (SPE-GC) analysis, we elucidated the composition and distribution of phytosterols in five rapeseed varieties and their corresponding processed oils and cakes. The results indicated that Zhongyou 516 and Xiwang 988 are richer in combined phytosterols, whereas Dadi 199, Zhongyouza 501, and Xiwang 291 have a greater concentration of free phytosterols. Steam explosion pretreatment significantly increased the extraction proportion of combined phytosterols in rapeseeds. Throughout the oil process, more than half of the total phytosterol content, specifically 57.0%, was transferred from the steam explosion-treated rapeseed into the rapeseed oil. The variety Xiwang 291 showed the highest efficiency in this transfer, achieving a rate of 61.7%. The study provides crucial data for the enhancement of rapeseed processing techniques and the efficient utilization of phytosterols. Moreover, the study highlights the potential use of the ratio of free to combined phytosterols as a discriminator for different rapeseed oil varieties, offering valuable insights for quality assurance and product differentiation in the industry.
本研究调查了汽爆预处理对油菜籽及其衍生产品中游离和组合植物甾醇分布的影响。利用固相萃取-气相色谱法(SPE-GC)分析,我们阐明了五个油菜籽品种及其相应加工油和油饼中植物甾醇的组成和分布。结果表明,中油 516 和西王 988 的组合植物甾醇含量较高,而大地 199、中油 501 和西王 291 的游离植物甾醇含量较高。蒸汽爆破预处理大大提高了油菜籽中组合植物甾醇的提取比例。在整个榨油过程中,植物甾醇总含量的一半以上(57.0%)从汽爆处理过的油菜籽转移到菜籽油中。品种西王 291 的转移效率最高,达到 61.7%。这项研究为提高油菜籽加工技术和植物甾醇的有效利用提供了重要数据。此外,该研究还强调了游离植物甾醇与组合植物甾醇之比作为不同菜籽油品种鉴别指标的潜在用途,为该行业的质量保证和产品差异化提供了有价值的见解。
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引用次数: 0
Influence of coffee roasting degree on antioxidant and metabolic parameters: Comprehensive in vitro and in vivo analysis 咖啡烘焙度对抗氧化剂和代谢参数的影响:体外和体内综合分析
IF 6.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2024.100861
Claudia I. Gamboa-Gómez , Juliana Morales-Castro , Jazel Barragan-Zuñiga , Mayra Denise Herrera , Alejandro Zamilpa-Álvarez , José Luis Gónzalez , Gerardo Martínez-Aguilar , Elisa P. Morales-Castro , Monica Anese , Marilisa Alongi
This study aimed to assess the impact of roasting degree on antioxidant and metabolic parameters in vitro and in vivo. In vitro, we evaluated radical scavenging, lipid peroxidation, and the activity of digestive enzymes (α-glucosidase, α-amylase, and lipase). In vivo, we first examined coffee's effect on carbohydrate and lipid absorption in healthy rats, followed by a chronic evaluation of metabolic disorders and antioxidant markers using a diet-induced obesity model. In vitro results revealed that increased roasting degree reduced the antioxidant capacity of coffee brews. All brews showed lower inhibition of α-glucosidase and α-amylase, and lipase inhibition compared to the positive control (acarbose or orlistat). In vivo, all roasting degrees consistently reduced postprandial glucose levels by 20%. Notably, coffee with a high roasting degree (HRD) decreased serum triglycerides (TG) by ∼44% after a lipid load, while other roasts did not. Chronic administration of unroasted (UN) or HRD coffee significantly reduced weight gain compared to the obese control (∼15% and ∼10%, respectively). Notably, all coffee samples improved lipid metabolism parameters. UN and HRD coffee significantly decreased adipocyte volume by 58% and 48%, respectively, compared to the obese control. Additionally, all groups exhibited less than 30% hepatic lipid droplets independent of roasting degree. HRD treatment notably increased liver catalase (CAT) activity and reduced lipid peroxidation in serum (∼90%), liver (∼59%), and adipose tissue (∼37%) compared to the obese control group. These findings suggest that HRD in coffee may confer certain biological advantages.
本研究旨在评估焙烧程度对体外和体内抗氧化剂和代谢参数的影响。在体外,我们评估了自由基清除、脂质过氧化和消化酶(α-葡萄糖苷酶、α-淀粉酶和脂肪酶)的活性。在体内,我们首先研究了咖啡对健康大鼠碳水化合物和脂质吸收的影响,然后利用饮食诱发肥胖模型对代谢紊乱和抗氧化标志物进行了慢性评估。体外实验结果表明,烘焙程度的增加会降低咖啡的抗氧化能力。与阳性对照(阿卡波糖或奥利司他)相比,所有咖啡对α-葡萄糖苷酶、α-淀粉酶和脂肪酶的抑制作用都较低。在体内,所有烘焙度都能将餐后血糖水平降低 20%。值得注意的是,高烘焙度(HRD)的咖啡在脂质负荷后能使血清甘油三酯(TG)降低 44%,而其他烘焙度的咖啡则不能。与肥胖对照组相比,长期饮用未烘焙(UN)或高烘焙度(HRD)咖啡可显著减少体重增加(分别为15%和10%)。值得注意的是,所有咖啡样品都改善了脂质代谢参数。与肥胖对照组相比,UN 和 HRD 咖啡分别使脂肪细胞体积明显减少了 58% 和 48%。此外,无论烘焙程度如何,所有组的肝脏脂滴都少于 30%。与肥胖对照组相比,HRD 处理显著提高了肝脏过氧化氢酶(CAT)活性,降低了血清(∼90%)、肝脏(∼59%)和脂肪组织(∼37%)中的脂质过氧化反应。这些研究结果表明,咖啡中的HRD可能具有某些生物学优势。
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引用次数: 0
Psychochemical changes and functional properties of organosulfur and polysaccharide compounds of black garlic (Allium sativum L.) 黑蒜(Allium sativum L.)有机硫和多糖化合物的心理化学变化和功能特性
IF 6.3 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2024.100717
Gemilang Lara Utama , Zahida Rahmi , Meli Puspita Sari , In-in Hanidah

Background

Black garlic is one of the functional food products made from garlic which is processed through aging to improve sensory value and nutritional quality. Aging conditions has a major impact on the psychochemical and functional properties changes of black garlic which is closely related to organosulfur compounds and polysaccharides as the largest component in garlic.

Scope and approach

The method used in this research is a systematic review with the aim of research to determine the relationship between reactions during aging and changes in organosulfur, polysaccharides and non-enzymatic browning product compounds as well as the function of black garlic by focusing on certain aspects of aging including temperature, humidity, time, microorganism activity, and pre-treatment application.

Key findings and conclusions

Maillard reaction and polysaccharide degradation are still be the dominant reactions and play an important role in black garlic production. High hydrostatic pressure pretreatment could maintains the quality of black garlic so that the black garlic has the same taste characteristics as black garlic in general. Antioxidant properties in black garlic shown increase during thermal treatment. In addition, it is known that the activity of microorganisms plays a role and being potential to increase the quality value of black garlic as well as the antimicrobial activity.

背景黑蒜是以大蒜为原料制成的功能性食品之一,通过老化加工可提高感官价值和营养质量。老化条件对黑蒜的心理化学和功能特性变化有重大影响,这与大蒜中最大的有机硫化合物和多糖密切相关。范围和方法本研究采用的方法是系统综述,目的是通过关注老化的某些方面,包括温度、湿度、时间、微生物活性和预处理应用,确定老化过程中的反应与有机硫、多糖和非酶褐变产物化合物的变化以及黑蒜功能之间的关系。高静压预处理可以保持黑蒜的品质,使黑蒜具有与一般黑蒜相同的口感特征。在热处理过程中,黑蒜中的抗氧化特性会增加。此外,众所周知,微生物的活性在提高黑蒜质量和抗菌活性方面发挥着作用和潜力。
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引用次数: 0
期刊
Current Research in Food Science
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