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Explainable machine learning for predicting the geographical origin of Chinese Oysters via mineral elements analysis 通过矿物元素分析预测中国牡蛎地理来源的可解释机器学习
IF 6.3 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2024.100738
Xuming Kang , Yanfang Zhao , Lin Yao , Zhijun Tan

The traceability of geographic origin is essential for guaranteeing the quality, safety, and protection of oyster brands. However, the current outcomes of traceability lack credibility as they do not adequately explain the model's predictions. Consequently, we conducted a study to evaluate the efficacy of utilizing explainable machine learning combined with mineral elements analysis. The study findings revealed that 18 elements have the ability to determine regional orientation. Simultaneously, individuals should pay closer attention to the potential risks associated with oyster consumption due to the regional differences in essential and toxic elements they contain. Light gradient boosting machine (LightGBM) model exhibited indistinguishable performance, achieving flawless accuracy, precision, recall, F1 score and AUC, with values of 96.77%, 96.43%, 98.53%, 97.32% and 0.998, respectively. The SHapley Additive exPlanations (SHAP) method was used to evaluate the output of the LightGBM model, revealing differences in feature interactions among oysters from different provinces. Specifically, the features Na, Zn, V, Mg, and K were found to have a significant impact on the predictive process of the model. Consistent with existing research, the use of explainable machine learning techniques can provide insights into the complex connections between important product attributes and relevant geographical information.

地理原产地的可追溯性对于保证牡蛎品牌的质量、安全和保护至关重要。然而,目前的溯源结果缺乏可信度,因为它们不能充分解释模型的预测。因此,我们开展了一项研究,评估利用可解释机器学习结合矿物元素分析的功效。研究结果表明,18 种元素具有确定区域方位的能力。同时,由于牡蛎所含的必需元素和有毒元素存在地区差异,人们应更密切地关注食用牡蛎的潜在风险。光梯度增强机(LightGBM)模型表现出无差别的性能,准确度、精确度、召回率、F1得分和AUC均无懈可击,其值分别为96.77%、96.43%、98.53%、97.32%和0.998。使用 SHapley Additive exPlanations(SHAP)方法评估了 LightGBM 模型的输出结果,发现了不同省份牡蛎之间特征交互作用的差异。具体而言,Na、Zn、V、Mg 和 K 等特征对模型的预测过程有显著影响。与现有研究一致,使用可解释机器学习技术可以深入了解重要产品属性与相关地理信息之间的复杂联系。
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引用次数: 0
Effect and potential mechanism of nitrite reductase B on nitrite degradation by Limosilactobacillus fermentum RC4 亚硝酸盐还原酶 B 对发酵柠檬酸乳杆菌 RC4 降解亚硝酸盐的影响和潜在机制
IF 6.3 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2024.100749
Qing Fan , Chaoran Xia , Xiaoqun Zeng , Zhen Wu , Yuxing Guo , Qiwei Du , Maolin Tu , Xinanbei Liu , Daodong Pan

Nitrite has the potential risk of hypoxic poisoning or cancer in pickled food. In our previous study, Limosilactobacillus fermentum (L. fermentum) RC4 is effective in nitrite degradation by producing nitrite reductase B (NirB). To investigate the detailed mechanism from the genome, response, and regulation of NirB, the whole-genome sequence of L. fermentum RC4 was analyzed, the L. fermentum-EGFP-nirB with enhanced green fluorescent protein (EGFP) labeled the nitrite reductase large subunit nirB, and the recombined L. fermentum-NirB with overexpression NirB strain was conducted. The key genes within the dominant metabolism pathways may be involved in stress tolerance to regulate the degrading process. The green fluorescence density of EGFP indicated that NirB activity has a threshold and peaked under 300 mg/L nitrite concentration. NirB overexpressed in L. fermentum RC4 boosted the enzyme activity by 39.6% and the degradation rate by 10.5%, when fermented in 300 mg/L for 40 h, compared to the control group. RNA-seq detected 248 differential genes mainly enriched in carbohydrate, amino acid, and energy metabolism. The ackA gene for pyruvate metabolism and the mtnN gene for cysteine metabolism were up-regulated. NirB regulates these genes to produce acid and improve stress resistance for L. fermentum RC4 to accelerate nitrite degradation.

亚硝酸盐在腌制食品中具有潜在的缺氧中毒或致癌风险。在我们之前的研究中,Limosilactobacillus fermentum(L. fermentum)RC4 通过产生亚硝酸盐还原酶 B(NirB)来有效降解亚硝酸盐。为了从基因组、反应和调控等方面研究 NirB 的详细机制,研究人员分析了 L. fermentum RC4 的全基因组序列,用增强绿色荧光蛋白(EGFP)标记亚硝酸盐还原酶大亚基 nirB,并重组 L. fermentum-NirB 过表达 NirB 菌株。主导代谢途径中的关键基因可能参与胁迫耐受性,以调控降解过程。EGFP 的绿色荧光密度表明,NirB 的活性有一个阈值,在亚硝酸盐浓度为 300 mg/L 时达到峰值。与对照组相比,过表达 NirB 的 L. fermentum RC4 在 300 mg/L 浓度下发酵 40 小时后,酶活性提高了 39.6%,降解率提高了 10.5%。RNA-seq 检测到 248 个差异基因,主要富集在碳水化合物、氨基酸和能量代谢中。丙酮酸代谢的 ackA 基因和半胱氨酸代谢的 mtnN 基因被上调。NirB 对这些基因进行调控,以产生酸并提高 L. fermentum RC4 的抗逆性,加速亚硝酸盐降解。
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引用次数: 0
A critical review of the bioactive ingredients and biological functions of camellia oleifera oil 油茶籽油的生物活性成分和生物功能综述
IF 6.3 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2024.100753
Peiju Qin , Junjun Shen , Jeigen Wei , Yuqi Chen

Camellia oleifera oil is a pure and natural high-grade oil prevalent in South China. Camellia oleifera oil is known for its richness in unsaturated fatty acids and high nutritional value. There is increasing evidence indicating that a diet rich in unsaturated fatty acids is beneficial to health. Despite the widespread production of Camellia oleifera oil and its bioactive components, reports on its nutritional components are scarce, especially regarding systematic reviews of extraction methods and biological functions. This review systematically summarized the latest research on the bioactive components and biological functions of Camellia oleifera oil reported over the past decade. In addition to unsaturated fatty acids, Camellia oleifera oil contains six main functional components contributing to its antioxidant, antibacterial, anti-inflammatory, antidiabetic, anticancer, neuroprotective, and cardiovascular protective properties. These functional components are vitamin E, saponins, polyphenols, sterols, squalene, and flavonoids. This paper reviewed the biological activity of Camellia oleifera oil and its extraction methods, laying a foundation for further development of its bioactive components.

山茶油是一种盛产于中国南方的纯天然高级油脂。山茶油以富含不饱和脂肪酸和营养价值高而著称。越来越多的证据表明,富含不饱和脂肪酸的饮食有益健康。尽管油茶籽油及其生物活性成分的生产非常广泛,但有关其营养成分的报道却很少,特别是有关提取方法和生物功能的系统综述。这篇综述系统地总结了近十年来有关山茶油生物活性成分和生物功能的最新研究报告。除不饱和脂肪酸外,油茶籽油还含有六种主要功能成分,具有抗氧化、抗菌、抗炎、抗糖尿病、抗癌、保护神经和心血管的功效。这些功能成分包括维生素 E、皂苷、多酚、甾醇、角鲨烯和类黄酮。本文综述了油茶籽油的生物活性及其提取方法,为进一步开发其生物活性成分奠定了基础。
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引用次数: 0
Analytical and numerical solutions of pore formation in elastic food materials during dehydration 脱水过程中弹性食品材料中孔隙形成的分析和数值解决方案
IF 6.3 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2024.100762
R.G.M. van der Sman , Michele Curatolo , Luciano Teresi

In this paper, we describe a model for pore formation in food materials during drying. As a proxy for fruits and vegetables, we take a spherical hydrogel, with a stiff elastic skin, and a central cavity filled with air and water vapour. The model describes moisture transport coupled to large deformation mechanics. Both stress and chemical potential are derived from a free energy functional, following the framework developed by Suo and coworkers. We have compared Finite Volume and Finite Element implementations and analytical solutions with each other, and we show that they render similar solutions. The Finite Element solver has a larger range of numerical stability than the Finite Volume solver, and the analytical solution also has a limited range of validity. Since the Finite Element solver operates using the mathematically intricate weak form, we introduce the method in a tutorial manner for food scientists.

Subsequently, we have explored the physics of the pore formation problem further with the Finite Element solver. We show that the presence of an elastic skin is a prerequisite for the growth of the central cavity. The elastic skin must have an elastic modulus of at least 10 times that of the hydrogel. An initial pore with 10% of the size of the gel can grow to 5 times its initial size. Such an increase in porosity has been reported in the literature on drying of vegetables, if a dense hard skin is formed, known as case hardening. We discuss that models as presented in this paper, where moisture transport is strongly coupled to large deformation mechanics, are required if one wants to describe pore/structure formation during drying and intensive heating (as baking and frying) of food materials from first principles.

本文描述了食品材料在干燥过程中形成孔隙的模型。作为水果和蔬菜的代表,我们采用了一种球形水凝胶,它有一层坚硬的弹性表皮和一个充满空气和水蒸气的中心空腔。该模型描述了与大变形力学耦合的水分传输。应力和化学势均由自由能函数导出,沿用了 Suo 及其同事开发的框架。我们比较了有限体积求解和有限元求解以及彼此的分析求解,结果表明它们的求解结果相似。与有限体积求解器相比,有限元求解器的数值稳定性范围更大,而分析解的有效范围也有限。由于有限元求解器使用的是数学上复杂的弱形式,我们以教程的方式向食品科学家介绍了该方法。随后,我们使用有限元求解器进一步探索了孔隙形成问题的物理原理。我们发现,弹性表皮的存在是中心空腔生长的先决条件。弹性表皮的弹性模量必须至少是水凝胶的 10 倍。初始孔隙大小为凝胶大小的 10%,但可以增长到初始大小的 5 倍。如果形成致密的硬表皮,即所谓的表皮硬化,这种孔隙率的增加在有关蔬菜干燥的文献中已有报道。我们认为,如果想从第一原理上描述食品材料在干燥和强化加热(如烘烤和油炸)过程中孔隙/结构的形成,就必须使用本文提出的模型,即水分传输与大变形力学的强耦合模型。
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引用次数: 0
Efficient recovery of functional biomolecules from shrimp (Litopenaeus vannamei) processing waste for food and health applications via a successive co-culture fermentation approach 通过连续共培养发酵方法从对虾(Litopenaeus vannamei)加工废弃物中高效回收功能性生物大分子,用于食品和健康应用
IF 6.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2024.100850
Varongsiri Kemsawasd , Weeraya Karnpanit , Sirinapa Thangsiri , Pairote Wongputtisin , Apinun Kanpiengjai , Chartchai Khanongnuch , Uthaiwan Suttisansanee , Chalat Santivarangkna , Suwapat Kittibunchakul
This study developed a food-grade fermentation process that efficiently isolated proteins and minerals from shrimp-processing waste (SPW). The in vitro antioxidant and enzyme inhibitory effects of SPW hydrolysates obtained from the fermentation process were investigated. SPW broths were prepared from the head (SPW-SH) and body carapace (SPW-SS) of Pacific white shrimp (Litopenaeus vannamei) and fermented using a 5-day successive co-culture fermentation approach with Bacillus amyloliquefaciens TISTR-1880 and Lactobacillus casei TBRC-388. This bacterial combination demonstrated optimal efficiency in extracting proteins (up to 93% deproteinization) and minerals (up to 83% demineralization) from SPW samples compared with other studied co-culture combinations. The resulting SPW-SH and SPW-SS hydrolysates were rich in proteins (∼70 and ∼59 g/100 g dry weight, respectively). They exhibited significantly enhanced antioxidant potential compared to their corresponding non-fermented controls at up to 2.3 and 3.7-fold higher, respectively as determined by the ORAC, FRAP, and DPPH radical scavenging assays. The two SPW hydrolysates also had significantly higher inhibitory activities against angiotensin-converting enzyme, α-amylase, and lipase than the controls, indicating their improved anti-hypertension, anti-diabetes, and anti-obesity properties, respectively; however, both SPW-SH and SPW-SS hydrolysates did not inhibit α-glucosidase at the tested concentrations. The SPW hydrolysates produced in this study showed high potential for use as functional ingredients in food and nutraceutical products. Knowledge gained from this study can promote the prospective valorization of industrial SPW as an inexpensive source of functional biomolecules for food-related applications using a fermentation approach. This will increase the commercial value of SPW and reduce the environmental impact.
本研究开发了一种食品级发酵工艺,可从虾加工废弃物(SPW)中有效分离出蛋白质和矿物质。研究了发酵过程中获得的 SPW 水解产物的体外抗氧化和酶抑制作用。从太平洋南美白对虾(Litopenaeus vannamei)的头部(SPW-SH)和体表(SPW-SS)制备 SPW 肉汤,并使用淀粉芽孢杆菌 TISTR-1880 和干酪乳杆菌 TBRC-388 进行为期 5 天的连续共培养发酵。与其他已研究过的共培养组合相比,这种细菌组合在从 SPW 样品中提取蛋白质(去蛋白率高达 93%)和矿物质(去矿物质率高达 83%)方面表现出最佳效率。所得的 SPW-SH 和 SPW-SS 水解产物含有丰富的蛋白质(每 100 克干重分别为 70 ∼ 70 克和 59 ∼ 59 克)。经 ORAC、FRAP 和 DPPH 自由基清除试验测定,与相应的非发酵对照组相比,它们的抗氧化潜力明显增强,分别高达 2.3 倍和 3.7 倍。两种 SPW 水解产物对血管紧张素转换酶、α-淀粉酶和脂肪酶的抑制活性也明显高于对照组,这分别表明它们具有更好的抗高血压、抗糖尿病和抗肥胖特性;不过,在测试浓度下,SPW-SH 和 SPW-SS 水解产物对α-葡萄糖苷酶均无抑制作用。本研究中生产的 SPW 水解产物在用作食品和营养保健品的功能成分方面具有很大的潜力。从本研究中获得的知识可促进利用发酵方法将工业刨花作为一种廉价的功能性生物大分子来源用于食品相关应用的前景。这将提高刨花的商业价值并减少对环境的影响。
{"title":"Efficient recovery of functional biomolecules from shrimp (Litopenaeus vannamei) processing waste for food and health applications via a successive co-culture fermentation approach","authors":"Varongsiri Kemsawasd ,&nbsp;Weeraya Karnpanit ,&nbsp;Sirinapa Thangsiri ,&nbsp;Pairote Wongputtisin ,&nbsp;Apinun Kanpiengjai ,&nbsp;Chartchai Khanongnuch ,&nbsp;Uthaiwan Suttisansanee ,&nbsp;Chalat Santivarangkna ,&nbsp;Suwapat Kittibunchakul","doi":"10.1016/j.crfs.2024.100850","DOIUrl":"10.1016/j.crfs.2024.100850","url":null,"abstract":"<div><div>This study developed a food-grade fermentation process that efficiently isolated proteins and minerals from shrimp-processing waste (SPW). The in vitro antioxidant and enzyme inhibitory effects of SPW hydrolysates obtained from the fermentation process were investigated. SPW broths were prepared from the head (SPW-SH) and body carapace (SPW-SS) of Pacific white shrimp (<em>Litopenaeus vannamei</em>) and fermented using a 5-day successive co-culture fermentation approach with <em>Bacillus amyloliquefaciens</em> TISTR-1880 and <em>Lactobacillus casei</em> TBRC-388. This bacterial combination demonstrated optimal efficiency in extracting proteins (up to 93% deproteinization) and minerals (up to 83% demineralization) from SPW samples compared with other studied co-culture combinations. The resulting SPW-SH and SPW-SS hydrolysates were rich in proteins (∼70 and ∼59 g/100 g dry weight, respectively). They exhibited significantly enhanced antioxidant potential compared to their corresponding non-fermented controls at up to 2.3 and 3.7-fold higher, respectively as determined by the ORAC, FRAP, and DPPH radical scavenging assays. The two SPW hydrolysates also had significantly higher inhibitory activities against angiotensin-converting enzyme, α-amylase, and lipase than the controls, indicating their improved anti-hypertension, anti-diabetes, and anti-obesity properties, respectively; however, both SPW-SH and SPW-SS hydrolysates did not inhibit α-glucosidase at the tested concentrations. The SPW hydrolysates produced in this study showed high potential for use as functional ingredients in food and nutraceutical products. Knowledge gained from this study can promote the prospective valorization of industrial SPW as an inexpensive source of functional biomolecules for food-related applications using a fermentation approach. This will increase the commercial value of SPW and reduce the environmental impact.</div></div>","PeriodicalId":10939,"journal":{"name":"Current Research in Food Science","volume":"9 ","pages":"Article 100850"},"PeriodicalIF":6.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266592712400176X/pdfft?md5=5fdfd4f6f23ff7424ae01a3ef7c2ef77&pid=1-s2.0-S266592712400176X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142315961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The inhibition effect and mechanism of typical hydrocolloids on the formation of heterocyclic amines: A study based on quantum chemical computation analysis 典型水胶体对杂环胺形成的抑制作用及其机理:基于量子化学计算分析的研究
IF 6.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2024.100862
Tiantian Huang, Pin Jiang, Tao Li, Guangyu Li, Yuyu He, Yuezheng Kuang, Yijie Wang
Hydrocolloids, as thickeners, have been receiving increasing attention from researchers. Although they exhibit significant free radical quenching abilities, which demonstrate potential heterocyclic amines (HAs) inhibitory capabilities by blocking the free radical pathway, the inhibitory effect and mechanism are still unclear. This study investigated the effects of three typical hydrocolloids (alginic acid, chitosan, and carrageenan) on both free and bound HAs in fried meatballs, along with their mechanisms of free radical quenching using density functional theory. The result showed that all three hydrocolloids can effectively inhibit the generation of HAs. The maximum inhibition rate reached 33.33% for free HAs and 23.18% for bound HAs. Phenylacetaldehyde, glyoxal and methyl glyoxal, were significantly inhibited, indicating that hydrocolloids alleviated the production of HAs by inhibiting the generation of intermediates. At moment, three hydrocolloids effectively inhibited the generation of total free radicals. Frontier orbital and density functional theory analysis revealed that carrageenan had the lowest HOMO-LUMO energy gaps, ionization potential, highest nucleophilic index, chemical potential, and was more likely to react with free radicals. The results of this study indicate that three hydrocolloids can effectively inhibit HAs and provide theoretical support for their applications in food processing and safety.
作为增稠剂,水胶体越来越受到研究人员的关注。虽然它们表现出明显的自由基淬灭能力,证明了杂环胺(HAs)通过阻断自由基通路而具有潜在的抑制能力,但其抑制作用和机制仍不清楚。本研究利用密度泛函理论研究了三种典型的水胶体(海藻酸、壳聚糖和卡拉胶)对炸丸子中游离和结合的 HAs 的影响及其淬灭自由基的机理。结果表明,这三种水胶体都能有效抑制 HAs 的生成。游离 HAs 的最大抑制率为 33.33%,结合 HAs 的最大抑制率为 23.18%。苯乙醛、乙二醛和甲基乙二醛都受到了明显的抑制,这表明水胶体通过抑制中间产物的生成来缓解 HAs 的生成。目前,三种水胶体有效地抑制了总自由基的生成。前沿轨道和密度泛函理论分析表明,卡拉胶的 HOMO-LUMO 能隙、电离电位最低,亲核指数和化学电位最高,更容易与自由基发生反应。研究结果表明,三种亲水胶体能有效抑制 HAs,为它们在食品加工和安全领域的应用提供了理论支持。
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引用次数: 0
Rheology of paste-like food inks for 3D printing: Effects of nutrient and water content 用于 3D 打印的糊状食品油墨的流变学:营养成分和水分含量的影响
IF 6.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2024.100847
Z.Y. Bugday , A. Venkatachalam , P.D. Anderson , R.G.M. van der Sman
This research delves into understanding the effects of composition on the rheological response of multi-component food inks for 3D food printing. Accordingly, the motivation is to decouple the nutrient and water content effects on the rheology. We formulated inks by combining pea fractions with water and employing a water-holding-capacity based hydration method. Rheology is characterized by steady shear rate and oscillatory strain amplitude sweeps. Strain sweep curves infer that the deformation response of all inks follows a similar trend, and samples sharing the same macronutrient formulation are mapped to a master curve after scaling with the elastic plateau modulus. Samples sharing the same macronutrient formulation mapped to a master curve after scaling with the elastic modulus. Shear rate testing showed that the inks were shear thinning yield stress materials. Shear rate sweeps also collapsed on a master curve scaled by the yield stress and critical shear rate on the y and x axes. The yield stress and the plateau modulus appeared to be controlled by the water content, while the shear and strain thinning exponents were independent of the formulations, inferring that the rheology is scaled by the water content while preserving the shear thinning response. Observing the independence of the rheological properties from the nutrient composition and scalability of the rheology by the water content provided a step forward in developing formulations with various nutrient content at desired ow properties, which promises personalized nutrition. Furthermore, the study shows the applicability of various rheological techniques, which are expected to contribute to the literature on the rheology of granular pastes.
这项研究旨在了解成分对用于 3D 食品打印的多组分食品油墨流变响应的影响。因此,研究的动机是解耦营养成分和水含量对流变性的影响。我们将豌豆馏分与水混合,并采用基于持水能力的水合方法配制油墨。流变学的特点是稳定的剪切速率和振荡的应变振幅扫描。应变扫描曲线推断出所有油墨的变形响应都遵循相似的趋势,具有相同宏量营养素配方的样品在与弹性高原模量缩放后映射到一条主曲线上。具有相同宏量营养素配方的样品在按弹性模量缩放后映射到一条主曲线上。剪切速率测试表明,油墨是剪切稀化屈服应力材料。剪切速率扫描也塌缩在一条主曲线上,该曲线在 y 轴和 x 轴上按屈服应力和临界剪切速率进行缩放。屈服应力和高原模量似乎由含水量控制,而剪切稀化指数和应变稀化指数则与配方无关,这推断出流变性是按含水量缩放的,同时保留了剪切稀化响应。观察到流变特性与营养成分无关,以及流变特性随含水量变化的可扩展性,为开发具有不同营养成分和所需欠缺特性的制剂迈出了一步,从而有望实现个性化营养。此外,该研究还显示了各种流变学技术的适用性,有望为颗粒糊剂流变学方面的文献做出贡献。
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引用次数: 0
Effective collection of protein-enriched cells from green tea residue: An innovative process for leaf protein production 从绿茶残渣中有效收集富含蛋白质的细胞:叶蛋白质生产的创新工艺
IF 6.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2024.100902
Chen Zhang , Ziyang He , Ankun Wang , Feipeng Zhang
Green tea residue (GTR) contains a high protein content. However, the protein in GTR can't be effectively extracted using traditional methods. Thus, a novel method using ethylenediamine tetraacetic acid (EDTA), ammonium oxalate, or Celluclast® 1.5 L were used to disperse leaf tissues and to collect mesophyll cells to enrich the protein. Compared with EDTA or ammonium oxalate treatment, Celluclast® 1.5 L treatment achieved the highest amounts of mesophyll cells, about 2.7 × 106 g−1 of GTR. The number of collected mesophyll cells was positively and linearly correlated with the extraction rate of glucose and xylose, indicating that cellulose and hemicellulose were key components influencing cell collection. Celluclast® 1.5 L treatment enriched the protein content by 1.65 times in collected mesophyll cells to 50% protein content with a protein recovery of 88%, providing a novel scheme to obtain high-quality leaf protein for the food industry.
绿茶渣(GTR)含有大量蛋白质。然而,传统方法无法有效提取绿茶渣中的蛋白质。因此,一种新方法使用乙二胺四乙酸(EDTA)、草酸铵或 Celluclast® 1.5 L 分散叶片组织并收集叶肉细胞以富集蛋白质。与乙二胺四乙酸或草酸铵处理相比,Celluclast® 1.5 L 处理获得的叶肉细胞数量最多,约为 2.7 × 106 g-1 GTR。收集到的叶肉细胞数量与葡萄糖和木糖的提取率呈线性正相关,表明纤维素和半纤维素是影响细胞收集的关键成分。Celluclast® 1.5 L 处理使收集的叶肉细胞中的蛋白质含量富集了 1.65 倍,蛋白质含量达到 50%,蛋白质回收率为 88%,为食品工业提供了一种获得高质量叶片蛋白质的新方案。
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引用次数: 0
The influence of cooperative fermentation on the structure, crystallinity, and rheological properties of buckwheat starch 协同发酵对荞麦淀粉结构、结晶度和流变特性的影响
IF 6.3 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2023.100670
Xia Li , Shanshan Wei , Zixin Gao, Ruixue Zhao, Zhanpeng Wang, Yuling Fan, Linlin Cui, Yuhua Wang

The effects of co-fermentation of yeast and Lactiplantibacillus plantarum 104 on buckwheat starch physical properties were investigated by various analytical techniques. To investigate the regulations of starch modification during fermentation and to provide a foundation for improving the performance of modified properties of buckwheat starch food. The pasting properties were decreased by co-fermentation also resulted in a reduction in the relative crystallinity. Scanning electron microscopy (SEM) demonstrated that more holes and a relatively rough granule surface were seen in the co-fermentation group. Fourier transform-infrared spectroscopy (FT-IR) results suggested that co-fermentation fermentation decreased the degree of short-range order (DO) and degree of t1he double helix (DD). The results demonstrated that co-fermentation altered these properties more rapidly than spontaneous fermentation. In conclusion, Lactiplantibacillus plantarum 104 could be used for buckwheat fermentation to improve food quality.

通过各种分析技术研究了酵母和植物乳杆菌 104 共同发酵对荞麦淀粉物理性质的影响。研究发酵过程中淀粉改性的规律,为改善荞麦淀粉食品的改性性能提供依据。共同发酵降低了糊化性能,也导致了相对结晶度的降低。扫描电子显微镜(SEM)显示,共同发酵组中出现了更多的孔洞,颗粒表面也相对粗糙。傅立叶变换红外光谱(FT-IR)结果表明,共同发酵降低了短程有序度(DO)和双螺旋度(DD)。结果表明,共同发酵比自发发酵更快地改变了这些特性。总之,植物乳杆菌 104 可用于荞麦发酵,以提高食品质量。
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引用次数: 0
Sprouting facilitates the antiglycative effect of black soybean (Glycine max (L.) Merr.) by promoting the accumulation of isoflavones 发芽可通过促进异黄酮的积累来增强黑大豆(Glycine max (L.) Merr.)的抗糖化作用
IF 6.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2024.100827
Qian Zhou , Yuxuan Chen , Lifang Peng , Jun Wu , Wen Hao , Mingfu Wang

The exposure of advanced glycation end products (AGEs) can induce chronic inflammation, oxidative stress, and accelerated aging, contributing the onset and progression of many diseases especially diabetic complications. Therefore, the searching of antiglycative foods is of practical significance, which may serve as a strategy in the attenuation of AGEs-associated diseases. In this study, we evaluated the antiglycative potential of some beans and bean sprouts that were common in our daily life. The results revealed that sprouting enhanced the antiglycative activity of beans, with black soybean sprouts demonstrating the highest efficacy (4.92-fold higher than the unsprouted beans). To assess practical implications, we examined the antiglycative activity of black soybean sprouts in pork soup, a popular food model that incorporates sprouts. Our findings confirmed the inhibitory effect on a dose-dependent manner. Through open column fractionation, we identified isoflavones and soyasaponin Bb as the candidates responsible for these effects. Additionally, compare to the unsprouted black soybeans, we found significant increases in the levels of antioxidative properties (2.51-fold), total phenolics (7.28-fold), isoflavones, and soyasaponin Bb during the sprouting process. Further studies determined that genistein, genistin, and daidzin were the major antiglycative compounds in black soybean sprouts. Collectively, this study emphasizes the benefits of sprouted beans and offers foundation for the development of functional sprouting foods.

暴露于高级糖化终产物(AGEs)会诱发慢性炎症、氧化应激和加速衰老,导致许多疾病尤其是糖尿病并发症的发生和发展。因此,寻找抗糖化食品具有重要的现实意义,可作为减轻 AGEs 相关疾病的一种策略。在这项研究中,我们评估了日常生活中常见的一些豆类和豆芽的抗糖化潜力。结果显示,发芽可增强豆类的抗糖化活性,其中黑黄豆芽的功效最高(比未发芽的豆类高出 4.92 倍)。为了评估实际意义,我们研究了黑黄豆芽在猪肉汤中的抗糖化活性,猪肉汤是一种很受欢迎的包含豆芽的食物模式。我们的研究结果证实,黑黄豆芽具有剂量依赖性的抑制作用。通过开柱分馏,我们确定了异黄酮和大豆皂苷 Bb 是产生这些效应的候选物质。此外,与未发芽的黑大豆相比,我们发现在发芽过程中,黑大豆的抗氧化性(2.51 倍)、总酚类物质(7.28 倍)、异黄酮和大豆皂苷 Bb 的含量均显著增加。进一步研究发现,黑黄豆芽中的主要抗糖化化合物是染料木素、染料木素和大豆皂甙。总之,这项研究强调了发芽豆的益处,并为开发功能性发芽食品奠定了基础。
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Current Research in Food Science
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