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Effects of crossbreeding on milk production of sheep 杂交对绵羊产奶量的影响
4区 农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-10-17 DOI: 10.17221/39/2023-cjas
Michaela Černá, Milan Margetín, Zdeňka Veselá, Michal Milerski
The aim of this study was to estimate the effects of crossbreeding on milk production and milk components in crossbred Tsigai, Lacaune, and Improved Wallachian sheep used to create a synthetic Slovak dairy sheep population. The local breeds Tsigai and Improved Wallachian were crossed with the high-yielding breed Lacaune to increase milk yield. The effects of crossbreeding were evaluated based on breed and heterosis effects. The database used consisted of 23 018 lactations from 15 888 ewes. The effects of crossbreeding on milk production and fat and protein content were estimated using the restricted maximum likelihood method in REMLF90. Fixed effects on milk yield were also studied. In ewes with a 100% heterosis effect (ewes from purebred parents of different breeds), the heterosis effect increased milk production by 11.642 kg, milk fat production by 0.772 kg, and protein production by 0.565 kg per milking period. The effect of the Tsigai breed on milk production was –21.98 kg compared to Lacaune, while for the Improved Wallachian breed, the value was –38.58 kg. The Tsigai direct breed effect reduced fat production by 1.56 kg and protein production by 1.34 kg. The Improved Wallachian direct breed effect was –2.71 kg for fat production and –2.27 kg for protein production.
目的ofÂ本研究wasÂ估计ofÂ杂交对产奶量和乳成分的影响inÂ杂交的Tsigai, Lacaune和改良瓦拉几亚羊使用toÂ创建aÂ合成斯洛伐克奶羊群体。将地方品种赤改、改良瓦拉几亚与高产品种拉贡toÂ杂交,提高产奶量。从品种效应和杂种优势效应两方面评价了ofÂ杂交育种的效果。使用的数据库包括来自15 888只母羊的of 23 018次泌乳。利用限制极大似然法in REMLF90估计ofÂ杂交对产奶量和脂肪、蛋白质含量的影响。还研究了固定效应对产奶量的影响。InÂ母羊具有a 100%杂种优势效应(母羊来自纯种母羊ofÂ不同品种),杂种优势效应使每挤奶期产奶量增加by 11.642 kg,乳脂产量增加by 0.772 kg,蛋白质产量增加by 0.565 kg。对产奶量的影响ofÂ与to laacaune相比was -21.98 kg,而对瓦拉几亚改良品种的影响值was -38.58 kg。赤改直接养殖效应使脂肪产量降低by 1.56 kg,蛋白质产量降低by 1.34 kg。改良瓦拉几亚直系品种对脂肪产量影响was -2.71 kg,对蛋白质产量影响-2.27 kg。
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引用次数: 0
Polymorphism of the PGLYRP1 gene, the value of selected performance and functional traits, and causes of culling in Holstein-Friesian red-white cows 黑马红白奶牛PGLYRP1基因多态性、所选性能和功能性状的价值及淘汰原因
4区 农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-10-16 DOI: 10.17221/194/2022-cjas
Piotr Sablik, Andrzej Dybus, Ewa Januś, Błażej Nowak, Wojciech Kruszyński, Anna Mucha
This research paper addresses the hypothesis that the peptidoglycan recognition protein 1 (PGLYRP1) gene polymorphism (Tyr76His; dbSNP ID: ss104796364) has an influence on some performance traits and causes of culling in Polish Holstein-Friesian red-white cows. The study involved 134 cows kept on a farm in the south-west of Poland. PGLYRP1 genotypes TT, CT, and CC were detected. It was shown that compared with cows with genotypes CT and TT, the individuals with genotype CC were characterised by higher lifetime yields and higher amounts of lactation milk, fat, and protein. A beneficial effect of genotype CC, compared with genotype TT, was also noted in the case of the lifespan and, consequently, the length of the productive life and the average number of lactations. Diseases of the musculoskeletal system (genotypes CC and TT) and disorders of the reproductive system (genotype CT) were the most common causes of culling. An essential practical observation was the potentially higher susceptibility of cows with genotype CC to mastitis, which resulted in the necessity to cull over one-fifth of the animals in this group. Simultaneously, no cows in this group were culled due to low performance or metabolic, gastrointestinal, and respiratory diseases. Therefore, the PGLYRP1 gene seems to be a promising potential herd health marker; however, to consider it the main gene, it is necessary to extend the investigations to include more individuals and other breeds of dairy cattle.
本文提出了肽聚糖识别蛋白1 (PGLYRP1)基因多态性(Tyr76His;dbSNP ID: ss104796364) hasÂ对某些性能性状的影响,并导致ofÂ扑杀inÂ波兰荷斯泰因-弗里舍红白奶牛。这项研究涉及134头奶牛,这些奶牛饲养在inÂ波兰西南部ofÂ的aÂ农场。检测PGLYRP1 TT、CT、CC基因型。结果表明,与CT和TT基因型奶牛相比,CC基因型奶牛具有byÂ更高的终身产奶量和ofÂ更高的泌乳量、脂肪量和蛋白质量。AÂ有益效果ofÂ基因型CC与基因型TT相比,也被注意到inÂ在ofÂ寿命的情况下,因此,在长度ofÂ生产寿命和平均产乳数ofÂ。疾病ofÂ肌肉骨骼系统(基因型CC和TT)和疾病ofÂ生殖系统(基因型CT)是最常见的原因ofÂ剔除。AnÂ重要的实际观察是潜在的高易感性ofÂ奶牛与基因型CC toÂ乳腺炎,这导致inÂ有必要toÂ扑杀超过五分之一ofÂ动物inÂ这一组。同时,由于toÂ生产性能低下或代谢、胃肠和呼吸系统疾病,没有奶牛inÂ该组被淘汰。因此,the PGLYRP1基因似乎toÂ是aÂ有希望的潜在畜群健康标记;然而,toÂ认为它是主要基因,有必要toÂ扩大调查toÂ包括更多的个体和其他品种ofÂ奶牛。
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引用次数: 0
Changes in the behaviour of Czech Fleckvieh dairy cows during the day under optimal ambient temperature conditions in the barn 在最佳环境温度条件下,白天捷克弗莱克维奶牛的行为变化
4区 农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-09-26 DOI: 10.17221/23/2023-cjas
Milan Večeřa, Stanislav Navrátil, Tomáš Kopec, Daniel Falta, Gustav Chládek, Francois Stefanus Lategan
In the experimental section of the barn (which made up 25% of the barn), 103 cubicles in free stall barn were selected in three rows, housing 98 dairy cows of Czech Fleckvieh cattle. Monitoring was done for one calendar year, once a week. From this period, 16 days with temperatures from 0.5–15.0 °C were selected for quantification. Observations were continuously recorded at 10:00 am (one hour after the coming of the last cow from the milking parlour), at hourly intervals until 7:00 pm. Ten observations were carried out every day of the monitoring period. In total, 15 680 individual observations were recorded. The microclimatic parameters (temperature, relative humidity) were monitored within the barns continuously at 15-minute intervals using three data logger sensors. Changes in the proportion of lying to standing dairy cows were observed during the day. A steady trend of a reduced ratio of lying dairy cows during the day (from 70% to 40%) in favour of standing dairy cows was observed. The optimal value of the cow comfort index – CCI (85%) was not reached in any monitored part of the day. The number of dairy cows lying on their left sides increased from 50.4% to 56%, especially after feeding (at 4:00 pm). Differences were found in the proportion of lying and standing cows (< 0.05) at an optimal microclimate during the day. A significant preference for the left side when lying down was also found.
InÂ实验区ofÂ牛棚(占牛棚的25% ofÂ), 103个隔间inÂ自由栏牛棚被选中inÂ三排,饲养奶牛98头ofÂ捷克弗列克维耶牛。监测为期一年,每周一次aÂ。从这段时间开始,选择温度在0.5-15.0 °C以下的16天进行量化。观察结果连续记录atÂ上午10:00 (ofÂ最后一头奶牛从挤奶室出来一小时后),atÂ每隔一小时记录一次,直到晚上7:00。在监测期间每天进行10次观测ofÂ。InÂ总计,15Â记录了680个个体观察。利用三个数据记录仪传感器连续监测畜棚内的微气候参数(温度、相对湿度)atÂ,每隔15分钟监测一次。白天观察奶牛的变化inÂ比例ofÂ卧卧toÂ站立。观察到奶牛产奶率AÂ稳定趋势of aÂ降低ofÂ(白天产奶率由70%降低到to 40%) inÂ有利于产奶率ofÂ。当天奶牛舒适指数- CCI(85%)未达到最优值of inÂ任何监测部分ofÂ。左侧卧的奶牛数量ofÂ从50.4%增加到to 56%,特别是在饲喂后(atÂ下午4:00)。躺牛和站牛的比例ofÂ差异显著(P < 0.05) atÂ是白天最适宜的小气候。AÂ我们还发现,当我们躺着的时候,我们更倾向于左侧。
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引用次数: 0
Gly-Leu instead of Gly promoted the proliferation and protein synthesis of chicken intestinal epithelial cells Gly- leu代替Gly促进鸡肠上皮细胞增殖和蛋白合成
4区 农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-09-26 DOI: 10.17221/213/2022-cjas
Jing Liang, Baolong Du, Minyan Wan, Yapeng Zhao, Defu Tang
Amino acids have positive regulatory effects on the function of intestinal epithelial cells (IEC), but in the field of animal nutrition, research on the regulatory effects of amino acids on IEC is still in the initial stages. This study aims to explore the effects of Gly, Gly-Gly, and Gly-Leu on IEC proliferation and their possible mechanisms. Chicken small intestinal epithelial cells were separated using the tissue block method, and other miscellaneous cells were removed for digestion and passage culture. The IEC were cultured in the medium containing 20 nmol/l Gly, Gly-Gly and Gly-Leu for 24 h, and the expression of enterokinase and cytokeratin in cells, the growth curve and activity of IEC, cell cycle, differentially expressed genes, mRNA expression, and protein expression levels of p-mTOR and p-S6K1 in IEC were detected. Enterokinase and cytokeratin were expressed specifically in IEC. The results of growth curve and MTT revealed that the cell viability of IEC was significantly increased after treatment with Gly, Gly-Gly and Gly-Leu. The cell cycle results showed that compared with the control group, Gly, Gly-Gly and Gly-Leu intervention could increase the proportion of IEC in G1 phase, and the proportion in S phase of IEC was decreased. Transcriptome sequencing showed that compared with the control group, there were 54, 28 and 30 differential genes in Gly group, Gly-Gly group and Gly-Leu group, respectively. These genes were mainly enriched in nitric oxide synthesis and protein kinase B signalling, PI3K signal and cellular amino acid biosynthesis and transport signal pathways. RT-PCR results showed that the mRNA expression levels of PCYT2, SPP1, EMC6, GRIA2 and PKD2 were consistent with the sequencing results. Western blot results showed that compared with the control group, the protein expression of p-mTOR and p-S6K1 in Gly group, Gly-Gly group and Gly-Leu group was significantly increased. Gly-Leu can promote the protein synthesis in IEC by activating protein synthesis of mTOR signalling pathway in chicken IEC.
氨基酸对theÂ功能ofÂ肠上皮细胞(IEC)具有正向调节作用,但inÂ在ofÂ动物营养学领域,对theÂ调节作用ofÂ氨基酸对IEC的研究仍处于inÂ初级阶段。本研究旨在toÂ探讨theÂ影响of Gly、Gly-Gly和Gly- leu对IEC增殖的影响及其可能的机制。采用theÂ组织块法分离鸡小肠上皮细胞,去除其他杂细胞forÂ消化传代培养。西娅IEC在培养中包含20 nmol / l g, Gly-Gly Gly-Leu论坛24 h,和西娅表达肠激酶和细胞角蛋白在细胞,西娅生长曲线和活动IEC,细胞周期,差异表达基因,p-mTOR gaozhisheng mRNA表达,蛋白表达水平和p-S6K1 inA IEC被检测到。肠激酶和细胞角蛋白特异性表达in IEC。TheÂ结果ofÂ生长曲线和MTT显示thatÂ细胞活力of IEC wasÂ经Gly、Gly-Gly和Gly- leu处理后显著提高。西娅细胞周期的结果显示,与西娅对照组相比,g, Gly-Gly和Gly-Leu干预可能会增加西娅IEC在G1期,比例和西娅在S期比例耀目IEC经济下降。转录组测序结果显示thatÂ与theÂ对照组相比,分别有54个、28个和30个差异基因in Gly组、Gly-Gly组和Gly- leu组。这些基因主要富集inÂ一氧化氮合成和蛋白激酶B信号通路、PI3K信号通路以及细胞氨基酸生物合成和转运信号通路。RT-PCR结果显示that mRNA表达水平of PCYT2、SPP1、EMC6、GRIA2和PKD2与theÂ测序结果一致。Western blot结果显示thatÂ与theÂ对照组相比,theÂ蛋白表达of p-mTOR和p-S6K1 in Gly组、Gly-Gly组和Gly- leu组wasÂ显著升高。Gly-Leu能促进theÂ蛋白质合成in IEC byÂ激活蛋白质合成of mTOR信号通路inÂ鸡IEC。
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引用次数: 0
Evaluation of crystallisation structures of cervical mucus in Zwartbles sheep with previous oestrus synchronisation 与先前发情同步的茨瓦布尔羊宫颈粘液结晶结构的评价
4区 农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-09-26 DOI: 10.17221/4/2023-cjas
Vojtěch Pešan, Zuzana Rečková, Martin Hošek, Radek Filipčík, Katarina Soušková, Tomáš Kopec, Martina Pešanová Tesařová
This study was conducted to determine the individual types of crystallisation structures of cervical mucus (arborisation phenomenon) in sheep and changes in the types of these structures during oestrus. A total of 80 ewes aged between two and eight years were included in the study. Oestrus synchronisation was performed using intravaginal sponges, and samples of cervical mucus were collected 55–57 h after the removal of intravaginal sponges and subsequently evaluated. The crystallisation structures of the cervical mucus of sheep exhibit the same sequence during oestrus as in cattle. The most frequently occurring types of crystallisation in the observed sheep included twig-shaped (33.33%), twig-shaped-clubmosses (24.00%) and clubmosses (14.67%). The least common type was fern frond crystallisation (1.33%). A statistically significant difference was found between the representation of individual types of crystallisation (< 0.01). As with cattle, it is possible to use the evaluation of the crystallisation of cervical mucus to determine the optimum time for insemination, especially in groups of animals with previous synchronisation/induction of oestrus. Establishing the correct time for insemination or natural mating with a ram makes it possible to optimise breeding management and improve pregnancy rates, which are crucial in breeding these seasonally polyoestrous animals. Based on the results, it can be stated that insemination should take place 57+ h after the removal of intravaginal sponges and the application of hormonal treatments for oestrus induction and synchronisation (equine chorionic gonadotropin).
本研究进行了toÂ确定个体类型ofÂ结晶结构ofÂ宫颈粘液(树突现象)inÂ羊,并在发情期改变inÂ类型ofÂ这些结构。AÂ总共of 80只2至8岁的母羊被纳入inÂ研究。使用阴道内海绵进行发情同步,并在取出ofÂ阴道内海绵55-57 h后收集样本ofÂ宫颈粘液并随后进行评估。结晶结构ofÂ宫颈粘液ofÂ羊在发情期表现出相同的顺序asÂ在牛。最常见的ofÂ结晶inÂ类型为细枝状(33.33%)、细枝状棒状苔藓(24.00%)和棒状苔藓(14.67%)。最不常见的是蕨类植物叶结晶(1.33%)。AÂ不同个体类型之间的表现有统计学差异of ofÂ结晶(P < 0.01)。AsÂ对于牛,可以toÂ使用评估ofÂ结晶ofÂ宫颈粘液toÂ确定最佳授精时间,特别是inÂ组ofÂ以前同步/诱导发情的动物ofÂ。确定与aÂ公羊进行授精或自然交配的正确时间,可以toÂ优化育种管理并提高怀孕率,这对于inÂ繁殖这些季节性多情动物至关重要。根据结果,可以认为,在取出ofÂ阴道内海绵并应用ofÂ诱导和同步发情的激素治疗(马绒毛膜促性腺激素)后57+ h应进行人工授精。
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引用次数: 0
In vitro assessment of the relationships between the digestion of different types of rice straw and bacterial community in the rumen 不同类型水稻秸秆消化量与瘤胃细菌群落关系的体外研究
4区 农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-09-26 DOI: 10.17221/193/2022-cjas
Ajmal Wali, Naoki Nishino
The aims of this study were to examine bacterial communities in relation to the rumen digestion of rice straw and to understand how concentrate supplements affect gut bacteria involving the digestion of a rice straw-based diet. The substrates were rice straw (RS) alone (experiment 1) and RS with 25% concentrates (barley and kidney beans) (experiment 2). The genomic DNA was collected to determine bacterial diversity by conducting denaturing gradient gel electrophoresis (DGGE). V6–V8 region group-specific (Clostridium and Bacteroides) primers were employed in the analyses. The DGGE band pattern was subjected to cluster analysis to demonstrate the similarity and difference between dietary treatments and solid-liquid fractions. Fibre digestibility, gas production, and volatile fatty acid (VFA) concentration were increased with incubation time. The differences between solid and liquid fractions were great in total bacteria, Clostridium, and Bacteroides communities. Clostridium and Bacteroides communities appeared unaffected by incubation time, whereas great differences existed between solid and liquid fractions throughout incubations (experiment 1). Barley and kidney bean supplements increased gas production and lowered rumen fluid pH, whereas changes in VFA concentration were significant only for kidney bean supplement. The Clostridium, and Bacteroides groups were affected by barley and kidney beans in the liquid fraction. However, the solid fraction was stable (experiment 2). These results indicate a rigid and stable community structure of Clostridium and Bacteroides groups involved in the digestion of rice straw-based diets in the rumen. Distinctive differences between solid and liquid fractions were described; hence, separate analyses of two fractions may greatly help understand the relationship between fermentation patterns and microbial communities in the rumen.
本研究的目的是toÂ检查细菌群落inÂ关系toÂ瘤胃消化ofÂ稻草和toÂ了解精料补充剂如何影响肠道细菌参与消化of aÂ以稻草为基础的饮食。底物为单独稻秆(实验1)和稻秆加25%浓缩物(大麦和芸豆)(experiment 2)。收集基因组DNA toÂ测定细菌多样性byÂ进行变性梯度凝胶电泳(DGGE)。采用V6-V8区域群特异性(梭状芽孢杆菌和拟杆菌)引物inÂ进行分析。对DGGE谱带进行toÂ聚类分析toÂ,分析饲粮处理与固液组分之间的异同。纤维消化率、产气量和挥发性脂肪酸(VFA)浓度随培养时间的延长而增加。固体和液体组分的总细菌、梭状芽孢杆菌和拟杆菌群落的差异为greatÂ。梭状芽孢杆菌和拟杆菌群落不受byÂ孵育时间的影响,而在整个孵育过程中,固体和液体组分之间存在很大差异(实验1)。大麦和芸豆添加物增加了瘤胃产气量,降低了瘤胃pH值,而in VFA浓度的变化仅在芸豆添加物中显著。TheÂ梭状芽孢杆菌和拟杆菌组影响byÂ大麦和芸豆inÂ液体部分。然而,固体部分是稳定的(实验2)。这些结果表明aÂ刚性和稳定的群落结构ofÂ梭状芽孢杆菌和拟杆菌群参与inÂ消化ofÂ水稻秸秆饲料inÂ瘤胃。描述了固体和液体馏分之间的显著差异;因此,单独分析ofÂ两个馏分可以极大地帮助了解发酵模式和微生物群落inÂ瘤胃之间的关系。
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引用次数: 0
Dynamics of occurrence of defective eggs in initial laying lines of hens 母鸡初始产蛋系缺陷蛋发生的动态
IF 1.2 4区 农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-08-21 DOI: 10.17221/54/2023-cjas
T. Kopec, L. Máchal, E. Tůmová, Z. Rečková, R. Filipčík
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引用次数: 0
Diversity of the bovine genes IRAK1 and IRAK4 in the Toll-like receptor signaling pathway 牛基因IRAK1和IRAK4在Toll样受体信号通路中的多样性
IF 1.2 4区 农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-08-21 DOI: 10.17221/137/2022-cjas
Ladislav Tichý, K. Novák, J. Kyselová, M. Přibáňová, J. Calta, L. Vostrý
{"title":"Diversity of the bovine genes IRAK1 and IRAK4 in the Toll-like receptor signaling pathway","authors":"Ladislav Tichý, K. Novák, J. Kyselová, M. Přibáňová, J. Calta, L. Vostrý","doi":"10.17221/137/2022-cjas","DOIUrl":"https://doi.org/10.17221/137/2022-cjas","url":null,"abstract":"","PeriodicalId":10972,"journal":{"name":"Czech Journal of Animal Science","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42371490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Heat stress affects the milk yield, milk composition, serum oxidative status, and metabolites of Holstein cows during mid-lactation 热应激影响泌乳中期荷斯坦奶牛的产奶量、乳成分、血清氧化状态和代谢物
IF 1.2 4区 农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-08-16 DOI: 10.17221/2/2023-cjas
Xue Chen, Yongqiang Li, Jun Xiao, Jianan Dong, Wei Zhao, Zhiyi Han, Liang Xin, G. Qin, Tao Wang, Y. Zhen, Zhe Sun, Xuefeng Zhang
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引用次数: 0
Ameliorative effect of yeast cell walls on broiler chickens' performance and gut health under coccidiosis challenge 酵母细胞壁对球虫病侵袭下肉鸡生产性能和肠道健康的改善作用
IF 1.2 4区 农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-08-09 DOI: 10.17221/24/2023-cjas
Shawkat Abdulrazaq M'Sadeq
{"title":"Ameliorative effect of yeast cell walls on broiler chickens' performance and gut health under coccidiosis challenge","authors":"Shawkat Abdulrazaq M'Sadeq","doi":"10.17221/24/2023-cjas","DOIUrl":"https://doi.org/10.17221/24/2023-cjas","url":null,"abstract":"","PeriodicalId":10972,"journal":{"name":"Czech Journal of Animal Science","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45555179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Czech Journal of Animal Science
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