Aryan R. Ganjo, Aveen N. Adham, H. Al-Bustany, S. Aka
Abstract Moringa oleifera seed and leaf are used traditionally for the treatment of various health problems (among others, hypertension, scrapes, skin infection, diabetes, genitourinary illnesses), and to boost the immune system, as well as to act as a contraceptive. In this study, the antibacterial activity of seed and leaf M. oleifera extracts on three-gram negative bacteria was investigated, and phytochemical analysis for the association of antibacterial activity with the active constituents in the plant was determined. Moreover, understanding of the mechanism of action was achieved by applying the Auto Dock Vina technique. The phytochemical screening of M. oleifera seed and leaf extracts exhibited the presence of alkaloids, carbohydrates, cardioactive glycosides, flavonoids, tannins, phenols, steroids and terpenoids. In silico results revealed that compounds (4-O-caffeoyl quinic acid, 4-(α-L-rhamnopyranosyloxyl)-benzylisothiocyanate); (Isoquercitrin, 4-(α-L-rhamnopyranosyloxy) benzyl glucosinolate); and (Astragalin, 4-(α-L-rhamnopyranosyloxy) benzyl glucosinolate) from leaf and seed have the highest binding affinity and very good interactions with Transcriptional Activator Protein (LasR), Klebsiella pneumoniae carbapenemase (KPC), and Malonyl-CoA-acyl carrier protein transacylase (FabD), respectively.
摘要辣木种子和叶子传统上用于治疗各种健康问题（包括高血压、擦伤、皮肤感染、糖尿病、泌尿生殖系统疾病），增强免疫系统，以及用作避孕药。在本研究中，研究了籽和叶提取物对3克阴性细菌的抗菌活性，并通过植物化学分析确定了抗菌活性与植物中活性成分的相关性。此外，通过应用Auto Dock Vina技术，实现了对作用机制的理解。对油籽和油叶提取物进行植物化学筛选，发现其含有生物碱、碳水化合物、强心苷、黄酮类化合物、单宁、酚类化合物、类固醇和萜类化合物。计算机模拟结果表明，化合物（4-O-咖啡酰基奎宁酸，4-（α-L-鼠李吡喃糖基氧基）-苄基异硫氰酸酯）；（异槲皮苷，4-（α-L-鼠李糖基氧基）苄基硫代葡萄糖苷）；和（黄芪，4-（α-L-鼠李糖基氧基）苄基硫代葡萄糖苷）分别与转录激活蛋白（LasR）、肺炎克雷伯菌碳青霉烯酶（KPC）和丙酰基辅酶A酰基载体蛋白转酰酶（FabD）具有最高的结合亲和力和非常好的相互作用。
{"title":"Phytochemical analysis and evaluation of antibacterial activity of Moringa oleifera extracts against gram-negative bacteria: an in vitro and molecular docking studies","authors":"Aryan R. Ganjo, Aveen N. Adham, H. Al-Bustany, S. Aka","doi":"10.2478/cipms-2022-0035","DOIUrl":"https://doi.org/10.2478/cipms-2022-0035","url":null,"abstract":"Abstract Moringa oleifera seed and leaf are used traditionally for the treatment of various health problems (among others, hypertension, scrapes, skin infection, diabetes, genitourinary illnesses), and to boost the immune system, as well as to act as a contraceptive. In this study, the antibacterial activity of seed and leaf M. oleifera extracts on three-gram negative bacteria was investigated, and phytochemical analysis for the association of antibacterial activity with the active constituents in the plant was determined. Moreover, understanding of the mechanism of action was achieved by applying the Auto Dock Vina technique. The phytochemical screening of M. oleifera seed and leaf extracts exhibited the presence of alkaloids, carbohydrates, cardioactive glycosides, flavonoids, tannins, phenols, steroids and terpenoids. In silico results revealed that compounds (4-O-caffeoyl quinic acid, 4-(α-L-rhamnopyranosyloxyl)-benzylisothiocyanate); (Isoquercitrin, 4-(α-L-rhamnopyranosyloxy) benzyl glucosinolate); and (Astragalin, 4-(α-L-rhamnopyranosyloxy) benzyl glucosinolate) from leaf and seed have the highest binding affinity and very good interactions with Transcriptional Activator Protein (LasR), Klebsiella pneumoniae carbapenemase (KPC), and Malonyl-CoA-acyl carrier protein transacylase (FabD), respectively.","PeriodicalId":11071,"journal":{"name":"Current Issues in Pharmacy and Medical Sciences","volume":"35 1","pages":"198 - 205"},"PeriodicalIF":0.3,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48182093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract The problem of skin repair under the conditions of systemic increased pressure in microvessels has a great importance. This is due to the fact that each year the incidence of this pathology has seen an increase during different operations, including plastic surgery. As the compensatory and reparative mechanisms occurring in the skin affected by vascular hyperbaria are still unidentified, the aim of the study was to investigate the features of the ultrastructure of the skin of white rats 30 days after modeling of portal hypertension. Objects of the study were anterior abdominal wall biopsies for electron microscopy research. Our work demonstrated that the skin in intact animals (control group) was of typical structure before the beginning of the experiment and 30 days after a sham operation. Following 30 days modeling of portal hypertension, the ultrastructure of the epidermis was found to be intact, only slight thickening of the horny layer was revealed. However, in the basal epidermocytes, signs of crypts formation by cytolemma were revealed. Moreover, in the epidermocytes of the spinous and granular layers, the mitochondria, endoplasmic reticulum channels and ribosomes were almost non-evident. In addition, in the cytoplasm of the fibroblasts, a moderate amount of freely located ribosomes and a moderate number of polymorphic mitochondria were detected, while the lumens of the capillaries of the papillary layer of the dermis were narrowed. We also saw that the swelling of the cytoplasm in endothelial cells resulted in the narrowing of the microvessels lumen. What is more, the subendothelial zone was expanded – which is indicative of endothelial desquamation. Beyond the aforementioned, the nuclei of the endothelial cells were well contoured and had signs of chromatin condensation. Endothelial cells with signs of apoptosis were detected as well.
{"title":"Features of the ultrastructure of the skin of white rats 30 days after modeling of portal hypertension","authors":"N. Hrytsevych, V. Vereschaka","doi":"10.2478/cipms-2022-0029","DOIUrl":"https://doi.org/10.2478/cipms-2022-0029","url":null,"abstract":"Abstract The problem of skin repair under the conditions of systemic increased pressure in microvessels has a great importance. This is due to the fact that each year the incidence of this pathology has seen an increase during different operations, including plastic surgery. As the compensatory and reparative mechanisms occurring in the skin affected by vascular hyperbaria are still unidentified, the aim of the study was to investigate the features of the ultrastructure of the skin of white rats 30 days after modeling of portal hypertension. Objects of the study were anterior abdominal wall biopsies for electron microscopy research. Our work demonstrated that the skin in intact animals (control group) was of typical structure before the beginning of the experiment and 30 days after a sham operation. Following 30 days modeling of portal hypertension, the ultrastructure of the epidermis was found to be intact, only slight thickening of the horny layer was revealed. However, in the basal epidermocytes, signs of crypts formation by cytolemma were revealed. Moreover, in the epidermocytes of the spinous and granular layers, the mitochondria, endoplasmic reticulum channels and ribosomes were almost non-evident. In addition, in the cytoplasm of the fibroblasts, a moderate amount of freely located ribosomes and a moderate number of polymorphic mitochondria were detected, while the lumens of the capillaries of the papillary layer of the dermis were narrowed. We also saw that the swelling of the cytoplasm in endothelial cells resulted in the narrowing of the microvessels lumen. What is more, the subendothelial zone was expanded – which is indicative of endothelial desquamation. Beyond the aforementioned, the nuclei of the endothelial cells were well contoured and had signs of chromatin condensation. Endothelial cells with signs of apoptosis were detected as well.","PeriodicalId":11071,"journal":{"name":"Current Issues in Pharmacy and Medical Sciences","volume":"35 1","pages":"157 - 162"},"PeriodicalIF":0.3,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43273830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marcin Kunecki, Tomasz Oleksy, J. Nozynski, P. Podolec, J. Biernat, M. Deja, K. Golba, W. Płazak
Abstract The consequences of myocardial infarction (MI) are an increasing problem worldwide. Despite spectacular progress in the invasive treatment of ischemic heart disease, the ability to limit the ischemia-reperfusion (I/R) injury remains largely unrealized. Recent studies have shown that stimulation of opioid receptors may confer a cardioprotective effect against I/R injury. Levosimendan, the inodilator, is indicated for the short-term treatment of acutely decompensated heart failure. We tested the hypothesis that levosimendan may provide cardioprotection in the opioid-like mechanism in the human myocardium.
{"title":"Levosimendan protects against ischemia – reperfusion injury in the human heart muscle. A pilot study","authors":"Marcin Kunecki, Tomasz Oleksy, J. Nozynski, P. Podolec, J. Biernat, M. Deja, K. Golba, W. Płazak","doi":"10.2478/cipms-2022-0038","DOIUrl":"https://doi.org/10.2478/cipms-2022-0038","url":null,"abstract":"Abstract The consequences of myocardial infarction (MI) are an increasing problem worldwide. Despite spectacular progress in the invasive treatment of ischemic heart disease, the ability to limit the ischemia-reperfusion (I/R) injury remains largely unrealized. Recent studies have shown that stimulation of opioid receptors may confer a cardioprotective effect against I/R injury. Levosimendan, the inodilator, is indicated for the short-term treatment of acutely decompensated heart failure. We tested the hypothesis that levosimendan may provide cardioprotection in the opioid-like mechanism in the human myocardium.","PeriodicalId":11071,"journal":{"name":"Current Issues in Pharmacy and Medical Sciences","volume":"35 1","pages":"219 - 223"},"PeriodicalIF":0.3,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43448244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Katarzyna Szklener, Anna Rodzajewska, Weronika Kuryło, S. Mańdziuk
Abstract Glioblastomas are the most common and most lethal forms of malignant primary brain tumor. We present a case report of a patient with III-grade glioma who achieved stable disease (SD) and clinical improvement after trametinib administration. We also report a review of the literature to Current Treatment Guidelines of Glioblastoma and new therapeutic strategies based on molecularly targeted therapy. Traditional treatments, including surgery, radiotherapy, and chemotherapy, have many limitations concerning the prognosis of patients with glioblastomas. Unfortunately, these tumors’recur after primary resection in the majority of cases. There is no standard therapy for recurrence of GBM. Targeted therapy offers a promising new treatment strategy. Regardless of those outstanding results much more can be done in the field of therapeutic options. Most urgent concerns include potent combining molecular targeted therapy with other types of treatments, selecting a group of patients for whom they turn out to be the most beneficial, and addressing adverse events of these molecules.
{"title":"New therapeutic strategies based on molecularly targeted therapy in glioblastoma – a case report and review of the literature","authors":"Katarzyna Szklener, Anna Rodzajewska, Weronika Kuryło, S. Mańdziuk","doi":"10.2478/cipms-2022-0036","DOIUrl":"https://doi.org/10.2478/cipms-2022-0036","url":null,"abstract":"Abstract Glioblastomas are the most common and most lethal forms of malignant primary brain tumor. We present a case report of a patient with III-grade glioma who achieved stable disease (SD) and clinical improvement after trametinib administration. We also report a review of the literature to Current Treatment Guidelines of Glioblastoma and new therapeutic strategies based on molecularly targeted therapy. Traditional treatments, including surgery, radiotherapy, and chemotherapy, have many limitations concerning the prognosis of patients with glioblastomas. Unfortunately, these tumors’recur after primary resection in the majority of cases. There is no standard therapy for recurrence of GBM. Targeted therapy offers a promising new treatment strategy. Regardless of those outstanding results much more can be done in the field of therapeutic options. Most urgent concerns include potent combining molecular targeted therapy with other types of treatments, selecting a group of patients for whom they turn out to be the most beneficial, and addressing adverse events of these molecules.","PeriodicalId":11071,"journal":{"name":"Current Issues in Pharmacy and Medical Sciences","volume":"35 1","pages":"206 - 211"},"PeriodicalIF":0.3,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48612350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alina Deyneka, V. Protska, I. Zhuravel, O. Kyslychenko
Abstract Cockscomb (Сelosia cristata (L.) Kuntze) is a well-known decorative plant belonging to the Amaranthaceae L. family. It is used in South East Asia folk medicine as an anesthetic, anti-microbial, hemostatic, tonic and anti-inflammatory drug. Some 11 to 12 fatty acids have been identified in cockscomb roots, leaves, stem, herb, flowers and seeds, and their quantitative content has been determined by gas chromatography. Accordingly, in all herb samples, unsaturated fatty acids dominated, forming 68.18 to 75.93% of total content. Mostly, these compounds were accumulated in the roots. Among the unsaturated fatty acids, linoleic acid prevailed in roots, stems, herb, flowers and seeds, whereas linolenic acid prevailed in the leaves. The highest content of linoleic acid (45.57±1.14%) was found in the seeds, while that of linolenic acid (53.63±0.53%) was found in the leaves. The obtained results will be applied in the development of drugs on the basis of cockscomb herb lipophilic fraction.
{"title":"The study of fatty acid composition in Cockscomb (Сelosia cristata (L.) Kuntze)","authors":"Alina Deyneka, V. Protska, I. Zhuravel, O. Kyslychenko","doi":"10.2478/cipms-2022-0032","DOIUrl":"https://doi.org/10.2478/cipms-2022-0032","url":null,"abstract":"Abstract Cockscomb (Сelosia cristata (L.) Kuntze) is a well-known decorative plant belonging to the Amaranthaceae L. family. It is used in South East Asia folk medicine as an anesthetic, anti-microbial, hemostatic, tonic and anti-inflammatory drug. Some 11 to 12 fatty acids have been identified in cockscomb roots, leaves, stem, herb, flowers and seeds, and their quantitative content has been determined by gas chromatography. Accordingly, in all herb samples, unsaturated fatty acids dominated, forming 68.18 to 75.93% of total content. Mostly, these compounds were accumulated in the roots. Among the unsaturated fatty acids, linoleic acid prevailed in roots, stems, herb, flowers and seeds, whereas linolenic acid prevailed in the leaves. The highest content of linoleic acid (45.57±1.14%) was found in the seeds, while that of linolenic acid (53.63±0.53%) was found in the leaves. The obtained results will be applied in the development of drugs on the basis of cockscomb herb lipophilic fraction.","PeriodicalId":11071,"journal":{"name":"Current Issues in Pharmacy and Medical Sciences","volume":"35 1","pages":"176 - 182"},"PeriodicalIF":0.3,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43232892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Eight drugs blocking beta-adrenergic receptors activity (acebutolol, alprenolol, atenolol, oxprenolol, labetalol, metoprolol, propranolol and sotalol) were investigated through the use of the thin-layer technique with its mobile phase containing surfactant. Assessment of the effect of surfactant presence and 1-propanol concentration in the mobile phase on the retention and separation of investigated solutes was then carried out wherein the effect of the surfactant concentration on the zone shape properties (asymmetry and tailing coefficient) was investigated. The method was applied for the quantitative analysis of the chosen solutes, and the LOD and LOQ values of chosen were determined. These were as follows: acebutolol – 1.11 and 3.36 μg/spot, metoprolol 1.45 μg/spot, 4.4 μg/spot. The chosen system is environmentally friendly due to using silica gel plates and only 5% of propanol in water.
{"title":"Determination of β-blocking receptor drugs in silica gel TLC systems with the mobile phase containing surfactant","authors":"Adam Traczuk, B. Polak","doi":"10.2478/cipms-2022-0037","DOIUrl":"https://doi.org/10.2478/cipms-2022-0037","url":null,"abstract":"Abstract Eight drugs blocking beta-adrenergic receptors activity (acebutolol, alprenolol, atenolol, oxprenolol, labetalol, metoprolol, propranolol and sotalol) were investigated through the use of the thin-layer technique with its mobile phase containing surfactant. Assessment of the effect of surfactant presence and 1-propanol concentration in the mobile phase on the retention and separation of investigated solutes was then carried out wherein the effect of the surfactant concentration on the zone shape properties (asymmetry and tailing coefficient) was investigated. The method was applied for the quantitative analysis of the chosen solutes, and the LOD and LOQ values of chosen were determined. These were as follows: acebutolol – 1.11 and 3.36 μg/spot, metoprolol 1.45 μg/spot, 4.4 μg/spot. The chosen system is environmentally friendly due to using silica gel plates and only 5% of propanol in water.","PeriodicalId":11071,"journal":{"name":"Current Issues in Pharmacy and Medical Sciences","volume":"35 1","pages":"212 - 218"},"PeriodicalIF":0.3,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48469233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oleksander Maslov, M. Komisarenko, Svitlana Ponomarenko, Darina Horopashna, T. Osolodchenko, Sergii Kolisnyk, L. Derymedvid, Zoia Shovkova, E. Akhmedov
Abstract The aim of the study. To determine phenolic and organic acids compound profiles, and the antioxidant, antibacterial and anti-inflammatory activities of raspberry leaf extract. Materials and methods. The object of the study was red raspberry leaf extract. The quantity of phenolic compounds was determined by applying a spectrophotometric method of analysis, whereas organic acids content was assessed by means of the alkalimetric method, while the antioxidant activity of the obtained extract was evaluated by employing the potentiometric method, and antibacterial and antifungal activity was ascertained through the wells method, and anti-inflammatory activity was found via carrageenan – induced paw edema assay. Results. The content of phenolic compounds was 18.45±0.37 mg/ml, catechins was 10.12±0.20 mg/ml, flavonoids was 3.32±0.07 mg/ml, hydroxycinnamic acids derivatives was 2.39±0.05 mg/ml and organic acids was 7.25±0.15 mg/ml. Moreover, the antioxidant activity was 76.11±1.48 mmol-equiv./mres dry, which was higher by 32.80% than the reference drug „Ascorutin”. Staphylococcus aureus bacteria was the most sensitive to the extract (25.00±0.00 mm), whereas Pseudomonas aeruginosa was the most resistant (21.67 ±0.66 mm). Treatment with red raspberry leaf extract at 1 ml/kg showed a significant edema reduction at 1, 2 and 3 h at 38.8, 41.8 and 48.8%, compared with the saline group. Conclusian. The present study indicated that red raspberry leaf extract possesses antioxidant, antibacterial and anti-inflammatory activities. Thus, red raspberry leaves are a promising source of bioactive substances that can be used for further developing medicines in the treatment and prevention of lifestyle diseases.
{"title":"Investigation the influence of biologically active compounds on the antioxidant, antibacterial and anti-inflammatory activities of red raspberry (Rubus idaeous l.) leaf extract","authors":"Oleksander Maslov, M. Komisarenko, Svitlana Ponomarenko, Darina Horopashna, T. Osolodchenko, Sergii Kolisnyk, L. Derymedvid, Zoia Shovkova, E. Akhmedov","doi":"10.2478/cipms-2022-0040","DOIUrl":"https://doi.org/10.2478/cipms-2022-0040","url":null,"abstract":"Abstract The aim of the study. To determine phenolic and organic acids compound profiles, and the antioxidant, antibacterial and anti-inflammatory activities of raspberry leaf extract. Materials and methods. The object of the study was red raspberry leaf extract. The quantity of phenolic compounds was determined by applying a spectrophotometric method of analysis, whereas organic acids content was assessed by means of the alkalimetric method, while the antioxidant activity of the obtained extract was evaluated by employing the potentiometric method, and antibacterial and antifungal activity was ascertained through the wells method, and anti-inflammatory activity was found via carrageenan – induced paw edema assay. Results. The content of phenolic compounds was 18.45±0.37 mg/ml, catechins was 10.12±0.20 mg/ml, flavonoids was 3.32±0.07 mg/ml, hydroxycinnamic acids derivatives was 2.39±0.05 mg/ml and organic acids was 7.25±0.15 mg/ml. Moreover, the antioxidant activity was 76.11±1.48 mmol-equiv./mres dry, which was higher by 32.80% than the reference drug „Ascorutin”. Staphylococcus aureus bacteria was the most sensitive to the extract (25.00±0.00 mm), whereas Pseudomonas aeruginosa was the most resistant (21.67 ±0.66 mm). Treatment with red raspberry leaf extract at 1 ml/kg showed a significant edema reduction at 1, 2 and 3 h at 38.8, 41.8 and 48.8%, compared with the saline group. Conclusian. The present study indicated that red raspberry leaf extract possesses antioxidant, antibacterial and anti-inflammatory activities. Thus, red raspberry leaves are a promising source of bioactive substances that can be used for further developing medicines in the treatment and prevention of lifestyle diseases.","PeriodicalId":11071,"journal":{"name":"Current Issues in Pharmacy and Medical Sciences","volume":"35 1","pages":"229 - 235"},"PeriodicalIF":0.3,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44226153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Piroxicam is a popular anti-inflammatory drug that displays palliative and antipyretic activity. Peppermint oil is a common flavoring used in foods and drinks. To investigate the defensive action of Peppermint oil against the hepatic and renal histological damage induced by Piroxicam in mice. Forty healthy adult Swiss albino mice of both sexes were categorized into 4 groups (10 mice in each group): Control group (I); Treatment group (II) – injected with Piroxicam 0.3 mg/kg/rat/day via intraperitoneal route for 28 days; Treatment group (III) – oral Peppermint oil 0.2 ml/kg/day by oral gavage 24 hours preceding each injection of Piroxicam; Treatment group (IV) oral Peppermint oil alone. Blood samples were withdrawn to estimate the hepatic and renal functions. Immediately after death, specimens of liver and kidney from the four groups were isolated and put in 10% concentration buffered formalin for 24 hours then prepared for light microscopic examination. There was a highly significant rise in the serum level of hepatic enzymes (alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase and total serum bilirubin) in the group treated with Piroxicam, as compared to the control group. These returned to near normal level in the group treated with Piroxicam and Peppermint oil. Liver samples of the treated mice showed ballooning degeneration of hepatocytes, small apoptotic hepatocytes and inflammatory cellular infiltration, whereas kidney sections revealed cystic dilatation of Bowman’s space, shrinkage of glomerular tuft and apoptosis of epithelial cells lining the tubules. In contrast, the addition of peppermint oil efficiently ameliorated the hepatic and renal tissue changes. Piroxicam induces hepatorenal toxicity as exhibited by histological, histochemical and biochemical findings. Peppermint oil shows ameliorative properties against the hepatorenal toxic effects induced by Piroxicam.
{"title":"Histological changes induced by Piroxicam on the hepatic and renal tissues of mice with and without administration of Peppermint oil","authors":"M. Al-Hamdany, Faten Al-Tai, H. Ismail","doi":"10.2478/cipms-2022-0033","DOIUrl":"https://doi.org/10.2478/cipms-2022-0033","url":null,"abstract":"Abstract Piroxicam is a popular anti-inflammatory drug that displays palliative and antipyretic activity. Peppermint oil is a common flavoring used in foods and drinks. To investigate the defensive action of Peppermint oil against the hepatic and renal histological damage induced by Piroxicam in mice. Forty healthy adult Swiss albino mice of both sexes were categorized into 4 groups (10 mice in each group): Control group (I); Treatment group (II) – injected with Piroxicam 0.3 mg/kg/rat/day via intraperitoneal route for 28 days; Treatment group (III) – oral Peppermint oil 0.2 ml/kg/day by oral gavage 24 hours preceding each injection of Piroxicam; Treatment group (IV) oral Peppermint oil alone. Blood samples were withdrawn to estimate the hepatic and renal functions. Immediately after death, specimens of liver and kidney from the four groups were isolated and put in 10% concentration buffered formalin for 24 hours then prepared for light microscopic examination. There was a highly significant rise in the serum level of hepatic enzymes (alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase and total serum bilirubin) in the group treated with Piroxicam, as compared to the control group. These returned to near normal level in the group treated with Piroxicam and Peppermint oil. Liver samples of the treated mice showed ballooning degeneration of hepatocytes, small apoptotic hepatocytes and inflammatory cellular infiltration, whereas kidney sections revealed cystic dilatation of Bowman’s space, shrinkage of glomerular tuft and apoptosis of epithelial cells lining the tubules. In contrast, the addition of peppermint oil efficiently ameliorated the hepatic and renal tissue changes. Piroxicam induces hepatorenal toxicity as exhibited by histological, histochemical and biochemical findings. Peppermint oil shows ameliorative properties against the hepatorenal toxic effects induced by Piroxicam.","PeriodicalId":11071,"journal":{"name":"Current Issues in Pharmacy and Medical Sciences","volume":"35 1","pages":"183 - 190"},"PeriodicalIF":0.3,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44232593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Prostate cancer (PCa) is the most common invasive malignancy for men in the USA. The incidence and mortality rates of PCa are significantly higher among African American men, as compared to those in Caucasian men. Betulinic acid (BA) is a penta-cyclic triterpenoid that is often found in the bark of several species of plants. It possesses a variety of biological activities, including anti-cancer activities. We examined the cytotoxic effects and endoplasmic reticulum (ER) stress induced by BA and its ionic derivatives with PCa cells derived from African Americans and Caucasian men. The viability of all PCa cells was reduced by the BA compounds, and the cytotoxicity of these BA compounds was independent of ethnicity and androgen dependency. The BA compounds induced modest effects on ER stress proteins when compared with ER stress inducers, tunicamycin and thapsigargin. The induction of glucose regulated protein 78 (GRP78) was largely correlated with the expression of C/EBP homologous protein (CHOP) and cleaved poly [ADP-ribose] polymerase (PARP)/caspase-3 in the PCa cells. In summary, our data demonstrated that BA compounds impaired the growth of PCa cells regardless of ethnicity – through GRP78- and CHOP-independent pathways.
{"title":"Betulinic acid and its ionic derivatives impaired growth of prostate cancer cells without induction of GRP78 and CHOP","authors":"Alexus Williams, Keshawna Smith, Zarin Bhuiyan, J. Phillips, Huan Zhao, Takayuki Nitta","doi":"10.2478/cipms-2022-0030","DOIUrl":"https://doi.org/10.2478/cipms-2022-0030","url":null,"abstract":"Abstract Prostate cancer (PCa) is the most common invasive malignancy for men in the USA. The incidence and mortality rates of PCa are significantly higher among African American men, as compared to those in Caucasian men. Betulinic acid (BA) is a penta-cyclic triterpenoid that is often found in the bark of several species of plants. It possesses a variety of biological activities, including anti-cancer activities. We examined the cytotoxic effects and endoplasmic reticulum (ER) stress induced by BA and its ionic derivatives with PCa cells derived from African Americans and Caucasian men. The viability of all PCa cells was reduced by the BA compounds, and the cytotoxicity of these BA compounds was independent of ethnicity and androgen dependency. The BA compounds induced modest effects on ER stress proteins when compared with ER stress inducers, tunicamycin and thapsigargin. The induction of glucose regulated protein 78 (GRP78) was largely correlated with the expression of C/EBP homologous protein (CHOP) and cleaved poly [ADP-ribose] polymerase (PARP)/caspase-3 in the PCa cells. In summary, our data demonstrated that BA compounds impaired the growth of PCa cells regardless of ethnicity – through GRP78- and CHOP-independent pathways.","PeriodicalId":11071,"journal":{"name":"Current Issues in Pharmacy and Medical Sciences","volume":"35 1","pages":"163 - 168"},"PeriodicalIF":0.3,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42919587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Traditional medicine has a lot to give towards the world’s health, especially now that limits to conventional pharmacology has occurred. New scientific methodologies may spark a rebirth in global health research and development if rich and developing countries pooled their research capacities with inequitable collaborations. The Cassytha Filiformis has many medicinal uses. It is a parasite plant that has been used for medicinal purposes and other ornamental purposes in many parts of the world, and has found employment in Siddha, European, Ayurveda and Chinese folk medicine. In this review, the ethnobotany, phytochemistry and pharmacological benefits of Cassytha filiformis are discussed.
{"title":"Review on Ethnobotany and phytochemistry of Cassytha filiformis","authors":"Nithya Rajamanickam, Bincy Raj, P. Tiwari","doi":"10.2478/cipms-2022-0031","DOIUrl":"https://doi.org/10.2478/cipms-2022-0031","url":null,"abstract":"Abstract Traditional medicine has a lot to give towards the world’s health, especially now that limits to conventional pharmacology has occurred. New scientific methodologies may spark a rebirth in global health research and development if rich and developing countries pooled their research capacities with inequitable collaborations. The Cassytha Filiformis has many medicinal uses. It is a parasite plant that has been used for medicinal purposes and other ornamental purposes in many parts of the world, and has found employment in Siddha, European, Ayurveda and Chinese folk medicine. In this review, the ethnobotany, phytochemistry and pharmacological benefits of Cassytha filiformis are discussed.","PeriodicalId":11071,"journal":{"name":"Current Issues in Pharmacy and Medical Sciences","volume":"35 1","pages":"169 - 175"},"PeriodicalIF":0.3,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42243757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：