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Flight muscle and flight activity of melon fly, Bactrocera cucurbitae (Diptera: Tephritidae) 瓜小实蝇(双翅目:绢蝇科)瓜蝇的飞行肌肉和飞行活动
Pub Date : 2021-07-09 DOI: 10.3329/DUJBS.V30I2.54644
F. Ferdousi, S. Sultana, T. Akter, Pinakshi Roy, S. Begum
The flight activity and flight muscle of the melon fly, Bactrocera cucurbitae (Coquillett) (Diptera: Tephritidae) were observed. The Tethered technique was used to observe the flight activity in this study. The flight activity, and wing and flight muscles were compared between male and female melon flies. The results indicate that the female was relatively better and strong flier than the male. The mean duration of the flight activity of the females was 13.90 min/hour and of the males was 7.12 min./hour. The mean length, width, volume of wings of the males were 6.07 mm, 2.67 mm and 10.99 mm³, respectively. On the other hand, the mean length, width and volume of the wings of females were 7.07 mm, 2.87 mm and 15.60 mm³, respectively. In case of wing muscles, the mean volume of dorsal longitudinal muscle (DLM) in male and female was found 5.20 mm³ and 5.67 mm³, respectively. The mean length of flight wing muscle of male and female was 2.22 and 2.23 mm, respectively and the mean breadth of male and female was 1.65 and 1.77 mm, respectively. Dhaka Univ. J. Biol. Sci. 30(2): 179-185, 2021 (July)
对瓜小蝇(双翅目:绢蝇科)的飞行活动和飞行肌肉进行了观察。本研究采用系留技术观察飞行活动。比较了雄性和雌性瓜蝇的飞行活动、翅膀和飞行肌肉。结果表明,雌蜂的飞行能力相对较好,雄蜂的飞行能力较强。雌性和雄性的平均飞行活动时间分别为13.90 min/h和7.12 min/h。雄翅平均长6.07 mm,宽2.67 mm,体积10.99 mm³。雌鸟翅膀平均长7.07 mm,宽2.87 mm,体积15.60 mm³。在翅膀肌肉方面,男性和女性背纵肌(DLM)的平均体积分别为5.20 mm³和5.67 mm³。雄性和雌性的飞行翼肌平均长度分别为2.22和2.23 mm,雄性和雌性的平均宽度分别为1.65和1.77 mm。达卡大学。科学通报,30(2):179- 185,2021 (7)
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引用次数: 0
Heavy metal concentration in commonly sold stem vegetables in Dhaka city market and probable health risk 达卡市市场常售茎类蔬菜重金属浓度及可能的健康风险
Pub Date : 2021-07-09 DOI: 10.3329/DUJBS.V30I2.54648
R. Sultana, A. Chamon, M. Mondol
The present study was carried out to assess the concentration of chromium (Cr), cadmium (Cd), lead (Pb), nickel (Ni), copper (Cu), zinc (Zn), iron (Fe) and manganese (Mn) in four stem vegetables such as Potato (Solanum tuberosum L.), Ginger (Zingiber officinale Roscoe), Garlic (Allium sativumL.) and Onion (Allium cepa L.) by using Atomic Absorption Spectrophotometer (AAS). Average daily intake (ADI), hazard quotient (HQ) and hazard index (HI) were also estimated to assess the human health risks posed by heavy metals from the consumption of the studied vegetables. The studied stem vegetable samples were collected in four phases from Kawran Bazar fresh vegetable market of Dhaka city of Bangladesh. Mean concentrations of Cr, Cd, Pb, Ni, Cu, Zn, Fe and Mn in the studied stem vegetables ranged between 0.88 to 2.35, 0.08 to 0.15, 0 to 2.25, 0 to 3.30, 5.70 to 9.85, 18.83 to 40.67, 50.60 to 456.38 and 9.23 to 852.35 mg/kg of dry weight, respectively. Mean concentration of maximum permissible limit (MPL) exceed in onion for Cr, in potato and onion for Pb and in ginger for Fe, Ni and Mn. Average daily intake was found to be lower than the maximum permitted tolerable daily intake in most cases except for Mn (26.475 mg/person/day) for consumption Ginger. Hazard quotient of Mn for dietary intake of Ginger (3.152) and hazard indices of Ginger (4.626), Garlic (1.183) and Onion (1.069) exceeded unity, signifying potential health risks from the dietary intake of these vegetables. This study suggests regular monitoring of heavy metals in vegetables to avoid the potential health hazards on human. Dhaka Univ. J. Biol. Sci. 30(2): 221-232, 2021 (July)
采用原子吸收分光光度法(AAS)测定了马铃薯(Solanum tuberosum L.)、生姜(Zingiber officinale Roscoe)、大蒜(Allium sativumL.)和洋葱(Allium cepa L.) 4种茎类蔬菜中铬(Cr)、镉(Cd)、铅(Pb)、镍(Ni)、铜(Cu)、锌(Zn)、铁(Fe)和锰(Mn)的含量。此外,还估计了平均每日摄入量(ADI)、危害商数(HQ)和危害指数(HI),以评估食用所研究蔬菜中重金属对人体健康造成的风险。所研究的茎类蔬菜样本是在孟加拉国达卡市Kawran Bazar新鲜蔬菜市场分四个阶段采集的。茎类蔬菜中Cr、Cd、Pb、Ni、Cu、Zn、Fe和Mn的平均含量分别为0.88 ~ 2.35、0.08 ~ 0.15、0 ~ 2.25、0 ~ 3.30、5.70 ~ 9.85、18.83 ~ 40.67、50.60 ~ 456.38和9.23 ~ 852.35 mg/kg干重。洋葱中铬、马铃薯和洋葱中铅、生姜中铁、镍、锰的平均浓度均超过最大允许限量。除了食用生姜的锰(26.475毫克/人/天)外,在大多数情况下,平均每日摄入量低于允许的最大每日可耐受摄入量。生姜、大蒜和洋葱的锰危害系数分别为4.626、1.183和1.069,锰的危害系数均超过1,表明食用这些蔬菜存在健康风险。本研究建议定期监测蔬菜中的重金属,以避免对人体健康的潜在危害。达卡大学。科学通报,30(2):221- 232,2021 (7)
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引用次数: 3
Anatomical changes in chickpea (Cicer arietinum L.) under aluminium stress condition 铝胁迫条件下鹰嘴豆解剖结构的变化
Pub Date : 2021-07-09 DOI: 10.3329/DUJBS.V30I2.54645
Rifat Samad, P. Rashid, J. Karmoker
An experiment was conducted to investigate the effect of aluminium (Al) toxicity on the anatomical changes in the root, stem and leaf of chickpea (Cicer arietinum L.) plants grown in sand culture. Toxicity of Al reduced the length of primary root and the number of lateral roots of chickpea than that of the control. Aluminium decreased the size and number of vessels in the root of chickpea. Larger area of sclerenchyma cells was noticed in the stem of Al-stressed plant. Number of palisade parenchyma was reduced in the leaf of chickpea. Aluminium treatment caused closure of stomata. Increased number of trichomes in chickpea leaves was also reported due to aluminium. Dhaka Univ. J. Biol. Sci. 30(2): 187-196, 2021 (July)
本试验研究了铝(Al)毒性对砂培鹰嘴豆根、茎、叶解剖结构变化的影响。与对照相比,Al毒性使鹰嘴豆的主根长度和侧根数量减少。铝降低了鹰嘴豆根部血管的大小和数量。al胁迫下植物茎部厚壁组织细胞面积较大。鹰嘴豆叶片栅栏薄壁组织数量减少。铝处理导致气孔关闭。鹰嘴豆叶片毛状体数量的增加也被报道是由于铝。达卡大学。自然科学30(2):187- 196,2021 (July)
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引用次数: 2
Leaf traits of sal (Shorea robusta Gaertn.) populations selected from different regions of Bangladesh 孟加拉国不同地区沙蚕(Shorea robusta Gaertn.)种群的叶片特征
Pub Date : 2021-07-09 DOI: 10.3329/DUJBS.V30I2.54656
Pragga Saha Sharmi, A. Kashem, H. Rahman, M. Z. Hossain
This study investigated the variation in leaf traits of Sal (Shorea robusta Gaertn.) by collecting leaf samples from the natural populations of Sal distributed in Madhupur National Park, Cumilla and Dinajpur areas of Bangladesh. Soil samples were collected at 0 - 10 cm depth near the base of the tree trunk of the respective plants selected for the collection of leaf samples. Soils were analyzed for moisture content, pH, electrical conductivity, available N, total P, and organic C contents. Data showed a range of variation in the responses of leaf traits of the three populations. However, none of the morphological and physiological traits showed significant differences except chlorophyll content (p = 0.0047). Conversely, significant difference appeared in most of the leaf anatomical properties such as stomatal pore index (p = 0.0369), open-close behavior (p = < 0.0001) and stomatal density (p = 0.0008) among the three forests. Stomatal density, pore index, and percent open stomata were higher in the Madhupur forest, while closed stomata were significantly higher in the Dinajpur forest. Thus, the present study indicated that although all leaf traits did not show a similar trend of response, leaf stomata were more responsive to different geographical distribution, which could be attributed to the variation in soil moisture conditions. Dhaka Univ. J. Biol. Sci. 30(2): 317-324, 2021 (July)
本研究通过采集分布在孟加拉国Madhupur国家公园、Cumilla和Dinajpur地区的Sal (Shorea robusta Gaertn.)自然种群的叶片样本,研究了Sal (Shorea robusta Gaertn.)叶片性状的变异。土壤样品采集于所选植物树干基部附近0 ~ 10 cm深度处。土壤的水分含量、pH值、电导率、速效氮、全磷和有机碳含量进行了分析。结果表明,3个群体对叶片性状的响应存在一定的差异。除叶绿素含量(p = 0.0047)外,其余形态和生理性状均无显著差异。相反,三种林分在气孔指数(p = 0.0369)、开闭行为(p = < 0.0001)和气孔密度(p = 0.0008)等大部分解剖性状上均存在显著差异。Madhupur林的气孔密度、气孔指数和开放气孔率显著高于Dinajpur林,而关闭气孔率显著高于Madhupur林。因此,本研究表明,尽管所有叶片性状的响应趋势并不相似,但叶片气孔对不同地理分布的响应更大,这可能与土壤水分条件的变化有关。达卡大学。科学通报,30(2):317- 324,2021 (7)
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引用次数: 0
Validation of patient health questionnaire-9 for assessing depression of adults in Bangladesh 评估孟加拉国成人抑郁症的患者健康问卷-9的有效性
Pub Date : 2021-07-09 DOI: 10.3329/DUJBS.V30I2.54652
Roufun Naher, Reza A Rabby, Feroz Sharif
Depression is one of the most widely recognized and frequently underdiagnosed and undertreated mental health issues around the world. The Patient Health Questionnaire-9 (PHQ-9) has been suggested as the best accessible screening and case-discovering instrument to measure the severity of depression. The investigation aimed to set the reliability and validity of the PHQ-9 for the adults in Bangladesh. A total of 321 Bangladeshi adults (170 males and 151 females) were the study participants. A standard process of three phases was followed to adapt PHQ-9 in Bangladesh. The first phase was forward translation, second phase was Focus Group Discussion and panel of expert's rating and third phase was back translation into original language, piloting and final field data collection. The results showed good reliability of the translated version; the total scale Cronbach’s alpha is 0.837, gender-wise 0.839 for males and 0.841 is for females; similarly, the Spearman-Brown Coefficient is 0.855, and the Guttman Split-half coefficient is 0.848, which indicate the high Split-half reliability as well. The content and construct validity suggest that the Bangla PHQ-9 is a valid tool to assess the depression of Bangladeshi adults. Therefore, the Bangla version of PHQ-9 gives an impression of being a reliable and valid instrument to assess and diagnose depression among Bangladeshi people. Dhaka Univ. J. Biol. Sci. 30(2): 275-281, 2021 (July)
抑郁症是世界上最被广泛认可,但经常被误诊和治疗不足的精神健康问题之一。患者健康问卷-9 (PHQ-9)被认为是衡量抑郁症严重程度的最佳筛选和病例发现工具。本研究的目的在于确定PHQ-9量表在孟加拉国成年人中的信度和效度。共有321名孟加拉国成年人(170名男性和151名女性)参与了这项研究。PHQ-9在孟加拉国采用了三个阶段的标准程序。第一阶段是向前翻译,第二阶段是焦点小组讨论和专家小组评分,第三阶段是回译成原语,试点和最终的实地数据收集。结果表明,译文具有良好的信度;总量表Cronbach 's alpha为0.837,性别方面,男性为0.839,女性为0.841;同样,Spearman-Brown系数为0.855,Guttman Split-half系数为0.848,表明Split-half信度也很高。内容和结构效度表明孟加拉PHQ-9是评估孟加拉成年人抑郁的有效工具。因此,孟加拉语版本的PHQ-9给人的印象是一个可靠和有效的工具来评估和诊断孟加拉国人的抑郁症。达卡大学。科学通报,30(2):275- 281,2021 (July)
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引用次数: 7
Somatic chromosome number and ploidy level in three Curcuma spp. from Bangladesh 孟加拉国3种姜黄属植物体细胞染色体数目及倍性水平
Pub Date : 2021-07-09 DOI: 10.3329/DUJBS.V30I2.54639
Ishrat Jahan Bonna, Sumana Akter, Syeda Sharmeen Sultana
Three Curcuma L. species were investigated cytogenetically which represent diversed staining pattern of heterochromatins in interphase nuclei and prophase chromosomes with orcein staining. Curcuma longa and C. caesia were found to possess 2n = 3x = 63 somatic chromosomes whereas 2n = 2x = 42 chromosome number in C. zedoaria is reported for the first time from Bangladesh. Total chromosome length recorded in C. longa, C. caesia and C. zedoaria were 145.08 ± 2.85 μm, 164.93 ± 4.29 μm and 97.78 ± 2.41 μm, respectively. This was the first attempt to measure the length of the chromosomes for these species. The experiment confirmed the basic chromosome number x = 21 with triploid (C. longa, C. caesia) and diploid (C. zedoaria) Curcuma plants. Polyploidy could be employed in the evolution and diversification of the genus Curcuma, which is an essential factor to characterize the species of this genus. Dhaka Univ. J. Biol. Sci. 30(2): 133-140, 2021 (July)
对三种姜黄进行了细胞遗传学研究,发现其间期细胞核和前期染色体的异染色质染色模式不同。姜黄(Curcuma longa)和姜黄(C. caesia)的染色体数为2n = 3x = 63条,而姜黄(C. zedoaria)的染色体数为2n = 2x = 42条,在孟加拉国首次报道。龙葵、茜草和zedoaria的染色体总长度分别为145.08±2.85 μm、164.93±4.29 μm和97.78±2.41 μm。这是第一次尝试测量这些物种的染色体长度。实验证实了三倍体姜黄(C. longa, C. caesia)和二倍体姜黄(C. zedoaria)植株的基本染色体数x = 21。多倍体可以用于姜黄属的进化和多样化,这是该属物种特征的重要因素。达卡大学。自然科学30(2):133-140,2021 (7)
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引用次数: 0
Growth and yield performance of strawberry (Fragaria ananassa) under various doses of vermicompost and NPK fertilizers grown at rooftop of Charfasson area in the southern parts of Bangladesh 孟加拉国南部Charfasson地区屋顶不同剂量蚯蚓堆肥和氮磷钾肥料对草莓生长和产量的影响
Pub Date : 2021-07-09 DOI: 10.3329/DUJBS.V30I2.54653
Moomtahina Rahman, Alauddin, Ahmz Ali
A pot experiment was carried out on the rooftop of a building at Charfasson town Bhola to evaluate the growth and yield performance of strawberry (Fragaria ananassa) as influenced by different doses of vermicompost and inorganic fertilizers in the winter season of 2020-2021. Each pot was filled with ten kg soil and arranged in a completely randomized design having seven treatments with three replications. Treatments were T1: Control (-VC), T2: 5 t VC/ha, T3: 10 t VC/ha, T4: 15 t VC/ha, T5: 20 t VC/ha, T6: 25 t VC/ha, and T7: 50% RDF NPK (40 - 15-25 kg/ha). Different agronomic parameters were measured at the intervals of 30, 60, 90, and 120 days after sowing of seeds. All the treatments of vermicompost showed better responses in agronomic parameters than the control treatment. Among them, T5 treatment tends to show significantly (p<0.05) higher plant height (18.5 cm), number of the leaf (54/plant), root length (15.50 cm), first flowering (at 61 days), fruit length (5.75 cm), fruit diameter (13.75 cm), number of fruits (15/plant), the average weight of fruit (14.50 g), the fresh yield of fruits (13.59 t/ha), fresh weight of root (10.41 g/plant), fresh weight of reproductive structure (3.12 g/plant), weight of the dry reproductive structure (3.12 g/plant) and benefit-cost ratio (9.37). T6 treatment showed significantly (p<0.05) higher leaf area (49.00 cm2), fresh weight of petiole (4,61 g/plant), the weight of dry root (2.20 g/plant), weight of dry leaf (5.87 g/plant), weight of dry petiole (1.42 g/plant) and weight of dry biomass (12.48 g/plant). T2, T3 and T4 treatments showed significantly (p<0.05) higher growth than T7 treatment. The overall findings revealed that the application of vermicompost (20 t/ha) in the strawberry plant might be a suitable method in rooftop gardening to achieve its better agronomic and yield parameters in the southern parts of Bangladesh. Dhaka Univ. J. Biol. Sci. 30(2): 283-292, 2021 (July)
在Bhola Charfasson镇的一座建筑物屋顶进行盆栽试验,以评估2020-2021年冬季不同剂量蚯蚓堆肥和无机肥料对草莓(Fragaria ananassa)生长和产量的影响。每盆填入10公斤土壤,按完全随机设计,7个处理,3个重复。处理分别为T1:对照(-VC), T2: 5 t VC/ha, T3: 10 t VC/ha, T4: 15 t VC/ha, T5: 20 t VC/ha, T6: 25 t VC/ha, T7: 50% RDF NPK (40 - 15-25 kg/ha)。播种后30、60、90、120 d分别测定不同农艺参数。蚯蚓堆肥各处理的农艺指标均优于对照处理。其中,T5处理的株高(18.5 cm)、叶数(54个/株)、根长(15.50 cm)、初花(61 d)、果长(5.75 cm)、果径(13.75 cm)、果数(15个/株)、果平均重(14.50 g)、果鲜产量(13.59 t/ha)、根鲜重(10.41 g/株)、生殖结构鲜重(3.12 g/株)、干生殖结构重量(3.12 g/株)和效益成本比(9.37)。T6处理显著(p<0.05)提高了叶片面积(49.00 cm2)、叶柄鲜重(4.61 g/株)、干根重(2.20 g/株)、干叶重(5.87 g/株)、干叶柄重(1.42 g/株)和干生物量重(12.48 g/株)。T2、T3和T4处理的生长均显著高于T7处理(p<0.05)。总体结果表明,在孟加拉国南部地区,施用蚯蚓堆肥(20 t/ha)可能是一种适合屋顶园艺的方法,可以获得更好的农艺和产量参数。达卡大学。科学通报,30(2):283- 292,2021 (7)
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引用次数: 2
Adaptation of the tool to measure parenting self-efficacy (topse) in Bangladesh 衡量父母自我效能(topse)的工具在孟加拉国的适应性
Pub Date : 2021-07-09 DOI: 10.3329/DUJBS.V30I2.54643
Nafiza Ferdowshi, M. A. Imran, Tasmim Alam Trishna
Measuring parental self-efficacy has influenced the children and their environment for reaching optimal development. The current study aimed to adapt the Tool to Measure Parenting Self-Efficacy (TOPSE) scale and to determine its psychometric properties for using in the context of Bangladesh. A total of 180 mothers of children aged 0 to 6 years were conveniently selected for this study. The TOPSE and Rosenberg’s Self-Esteem scale were used as measuring instruments. Collected data were analyzed to determine psychometric properties by using Cronbach’s alpha, Pearson product moment correlation coefficient, item analysis, and factor analysis. TOPSE had acceptable internal consistency (Coefficient alpha=0.89) and test-retest reliability (r=0.96). The convergent validity of TOPSE showed a highly positive correlation (r=0.91) with the Bangla Rosenberg’s Self-Esteem scale. The factor analysis resulted in 4 factors with Eigenvalues > 1, explaining 87% of the variances and the four factors showed Cronbach’s coefficient values ranging from 0.50 to 0.99. TOPSE has reasonably good psychometric properties. Further research may wish to require to see the implication of TOPSE in evaluating parenting programs in Bangladesh. Dhaka Univ. J. Biol. Sci. 30(2): 169-177, 2021 (July)
父母自我效能感的测量对儿童及其环境达到最佳发展有影响。目前的研究旨在调整育儿自我效能测量工具(TOPSE)量表,并确定其心理测量特性,以便在孟加拉国的背景下使用。本研究方便地选取了180名0 - 6岁儿童的母亲。采用TOPSE和Rosenberg自尊量表作为测量工具。采用Cronbach’s alpha、Pearson积矩相关系数、项目分析和因子分析对收集到的数据进行分析,以确定心理测量特性。TOPSE具有可接受的内部一致性(系数α =0.89)和重测信度(r=0.96)。TOPSE的收敛效度与Bangla Rosenberg自尊量表呈高度正相关(r=0.91)。因子分析发现有4个因子特征值> 1,解释了87%的方差,4个因子的Cronbach系数值在0.50 ~ 0.99之间。TOPSE具有相当好的心理测量特性。进一步的研究可能需要看到TOPSE在评估孟加拉国的育儿计划中的意义。达卡大学。自然科学,30(2):169-177,2021 (7)
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引用次数: 1
Bird diversity, composition and response during COVID-19 in an urban landscape, Jamalpur, Bangladesh 孟加拉国贾马尔布尔,城市景观中2019冠状病毒病期间鸟类的多样性、组成和应对
Pub Date : 2021-07-09 DOI: 10.3329/DUJBS.V30I2.54651
A. R. Shome, M. Jaman, Fazle Rabbe, M. Alam
The world has been encountering a COVID-19 pandemic since late 2019. The world’s people have also witnessed the free movement of wildlife, even in many urban areas in some countries during the imposed lockdown. We conducted research on the birds in the urban landscape of Jamalpur Sadar upazila from March 2020 to October 2020 during this pandemic situation. A total of 134 species of birds with 4338 individuals were recorded during the study period. The number of resident bird species was the maximum (115 species, 85.82%) compared to migrants. The highest number of birds was observed (120 species, 89.55%) and counted 2278 individual birds 52.51%) in the summer season. It was observed that the highest diversity of birds (89 species, 65.92%) was in March. The tree was the most (94 species, n = 2502) used microhabitat by birds in the study area. Among all birds, Pycnonotus cafer was the most abundant bird species with the highest relative abundance (6.11%). Some bird species (e.g. Metopidius indicus, Anastomus oscitans, Amaurornis phoenicurus, Streptopelia tranquebarica, Lonchura malacca) were frequently observed in the urban landscape of the study area during the pandemic. Proper management and awareness creation are essential for the conservation of the bird species in this area. Dhaka Univ. J. Biol. Sci. 30(2): 261-274, 2021 (July)
自2019年底以来,世界一直在遭遇COVID-19大流行。世界人民也目睹了野生动物的自由活动,即使在实施封锁期间,一些国家的许多城市地区也是如此。在此次大流行期间,我们于2020年3月至2020年10月对贾马尔布尔·萨达尔·乌帕齐拉城市景观中的鸟类进行了研究。研究期间共记录鸟类134种4338只。留鸟种类最多,为115种,占候鸟种类的85.82%。鸟类数量以夏季最多(120种,占89.55%),单鸟2278只(占52.51%)。3月份鸟类多样性最高(89种,占65.92%);该树种是研究区鸟类利用最多的微生境(94种,n = 2502)。在所有鸟类中,白头翁的相对丰度最高(6.11%),是最丰富的鸟类。在大流行期间,在研究区城市景观中经常观察到一些鸟类(如:indicus Metopidius、thesucitans、Amaurornis phoenicurus、Streptopelia tranquebarica、Lonchura malacca)。适当的管理和提高意识对保护该地区的鸟类物种至关重要。达卡大学。科学通报,30(2):261- 274,2021 (7)
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引用次数: 2
Expression of heat shock protein genes Aaehsp26, Aaehsp83 and Aaehsc70 in response to thermal stress in Aedes aegypti larvae 热休克蛋白基因Aaehsp26、Aaehsp83和Aaehsc70在埃及伊蚊幼虫热应激中的表达
Pub Date : 2021-07-09 DOI: 10.3329/DUJBS.V30I2.54649
Hafisha Khatun Anee, Ashfaqul Muid Khandaker, R. Begum, R. Shahjahan
Climate change is responsible to a certain extent for the occurrence and spread of arboviral pathogens worldwide. Temperature is one of the crucial abiotic factors influencing the physiological processes of mosquitoes. Several genes of heat shock protein (AaeHsp26, AaeHsp83, and AaeHsc70) families are known to be expressed in mosquitoes, which aid in overcoming stress induced by elevated temperature. In this study, the relative expression of heat shock protein genes has been examined using Quantitative Real-time PCR (qPCR). The temperatures used for heat shock treatment were 27(control), 37, and 42°C for 1 hour heat shock period and applied to 3rd instar larvae. Significant up-regulation has been seen at 37, and 42°C. The highest expression level, about 82.43 fold, was reported for the AaeHsc70 gene at 42°C followed by 78.36 fold for AaeHsp26 at 37°C and 4.79 fold for AaeHsp83 at 42°C. The current study has shown that HSPs are important markers of stress and may function as critical proteins to protect and enhance the survival of Ae. aegypti larvae and pupae. Biological implications of these findings could impact the vector competencies Dhaka Univ. J. Biol. Sci. 30(2): 233-241, 2021 (July)
气候变化在一定程度上导致了虫媒病毒病原体在世界范围内的发生和传播。温度是影响蚊虫生理过程的重要非生物因素之一。已知热休克蛋白(AaeHsp26、AaeHsp83和AaeHsc70)家族的几个基因在蚊子中表达,这些基因有助于克服高温引起的应激。本研究利用实时荧光定量PCR (Quantitative Real-time PCR, qPCR)检测了热休克蛋白基因的相对表达。热休克处理温度分别为27℃(对照)、37℃和42℃,热休克期为1 h,分别施用于3龄幼虫。在37°C和42°C时可以看到显著的上调。AaeHsc70基因在42°C时的表达量最高,约为82.43倍,其次是AaeHsp26基因在37°C时的表达量为78.36倍,AaeHsp83基因在42°C时的表达量为4.79倍。目前的研究表明,热休克蛋白是应激的重要标志,可能是保护和提高Ae存活的关键蛋白。埃及伊蚊幼虫及蛹。这些发现的生物学意义可能会影响媒介能力。科学通报,30(2):233-241,2021 (7)
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引用次数: 0
期刊
Dhaka University Journal of Biological Sciences
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