Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA最新文献

Objective: To analyze the causes and evaluate the therapies for simple rhinologic headache resulting from rhinal structural abnormalities.

Method: A retrospective analysis of 62 cases of simple rhinologic headache was conducted. All the patients were examined by high-resolution CT and nasal endoscopy to identify the anatomic structural abnormality in the nasal cavity. After establishment of the diagnosis, the nasal abnormalities were corrected by nasal endoscopic surgery.

Result: Of all the cases, 46 were cured, 12 responded favorably to the treatment, and 4 failed to respond possibly due to multiple causes of the headache and misdiagnosis.

Conclusion: High-resolution CT combined with nasal endoscopy may improve the diagnosis of simple rhinologic headache and nasal endoscopic surgery may serve as an effective modality for its treatment by correcting the nasal structural abnormalities.

High-performance liquid chromatography with evaporative light-scattering detection (HPLC-ELSD) was employed for determination of amygdalin content in Semen Armeniacae Amarum. The detection was performed with the column of Hypersil-ODS (4.6 mm x 250 mm, 5 microm) and column temperature of 25 degrees C. The mobile phase was methanol-water (70:30) with flow rate of 0.5 ml/min. Evaporative light-scattering detector was used and the drift tube temperature was set at 98 degrees Celsius with the gas flow rate of 3.2 L/min. A standard curve was generated, which was linear in the range of 1.0-15.1 microg for amygdalin content (r=0.999 9). The average recovery of amygdalin was 99.0% with RSD of 2.9% (n=5). Besides simplicity and rapidness, the method yields accurate and reproducible results and can therefore be used in the quality control of Semen Armeniacae Amarum.

Objective: To evaluate the value of laminar flow in the treatment of burns.

Methods: The air in the laminar flow chamber and the wound tissues of the patients were sampled for bacterial detection. The number and stains of bacterial colony from different classes of laminar air flow chambers at different time points were inspected and compared.

Results: The bacterial number was 0 in the laminar flow chamber of 1000 grade, which was obviously different from that in the public area. The mortality was obviously decreased in the laminar air flow chamber with shorter treatment time and hospitalization. No wound infection occurred and the wounds healed smoothly in all these patients.

Conclusion: The application of laminar air flow can be helpful for the treatment of severe burns.

Objective: To investigate the effects of mitomycin magnetic polybutylcyanoacrylate nanoparticles (MMC-MPBCA- NP), magnetic polybutylcyanoacrylate nanoparticles (MPBCA-NP) and magnetic fluids (MF) and MMC on the proliferation and apoptosis of normal human liver cell strain (L-02 cells) in vitro.

Methods: The effect of MMC, MMC-MPBCA-NP, MPBCA-NP, and MF on the apoptosis and cell cycle of L-02 cells was studied by flow cytometry. The changes in the cell proliferation in response to treatments of the preparations and agents were examined by MTT colorimetric assay.

Results: MMC, MMC-MPBCA-NP, MPBCA-NP produced inhibition of the cell proliferation to different degrees but MF failed to result in cell proliferation inhibition. The results of MTT assay showed that MM, MPBCA-NP and MMC-MPBCA-NP all possessed concentration-dependent cytotoxicity, which increased with the concentration increment in comparison with the blank control.

Conclusion: The descending order of the 4 agents in their effect of L-02 cell strain growth inhibition is MF >MPBCA-NP>MMC-MPBCA-NP>MMC. MMC and MMC-MPBCA-NP can inhibit the proliferation and cause cell cycle changes of L-02 cells, while MPBCA-NP inhibits the cell proliferation without inducing cell cycle alterations.

Objective: Detected in non-transformed bone marrow-derived macrophages (BMDM) and identified as one of the key channels in modulating macrophage proliferation, activation and apoptosis, Kir2.1 channel is also characterized to play a crucial role in cell differentiation. The purpose of this study was to investigate the expression of Kir2.1 channel mRNA and protein during human monocyte-derived macrophage differentiation into foam cells.

Methods: Human peripheral blood monocytes were isolated from healthy male volunteers by density gradient centrifugation and then by adherence method. The macrophages identified as a homogeneous population of adherent cells were obtained after 5 days of culture. Expression of Kir2.1 channel during human macrophage differentiation into foam cells was investigated by RT-PCR, Western blotting and immunocytochemistry, respectively.

Results: After incubation of the macrophages with 30 mg/L OxLDL at 37 degrees C for 60 h, the cells were obviously enlarged in size and numerous red lipid granules observed under optical microscope. The cellular contents of the total cholesterol (TC), free cholesterol (FC) and cholesterol ester (CE) were markedly increased from 54.79+/-28.304 mg/g, 47.968+/-26.787 mg/g and 6.822+/-3.437 mg/g to 229.775+/-57.453 mg/g, 96.241+/-24.003 mg/g and 133.535+/-36.292 mg/g, respectively; the CE/TC ratio rose from (14.437+/-6.781)% to (57.946+/-3.507)% (n=7, P<0.05), suggesting the phenotype of foam cells. However, there was no significant difference in the relative expression of Kir2.1 channel mRNA between the macrophages and foam cells [(59.074+/-10.566)% vs (46.98+/-12.527)%, n=5, P>0.05], nor was there significant difference in the relative expression of Kir2.1 channel protein between them [(60.527+/-18.621)% vs (50.243+/-11.583)%, n=6, P>0.05].

Conclusion: Incubation of human monocyte-derived macrophages with 30 mg/L OxLDL for 60 h induces the differentiation of the cells into foam cells, but the expression of Kir2.1 channel does not change obviously.

Objective: To compare the block characteristics of levobupivacaine and bupivacaine for hypobaric unilateral spinal block.

Methods: Sixty patients undergoing hypobaric unilateral spinal block for lower extremity operations were randomized into 2 equal groups. In one group the patients were asked to lie on the uncompromised side for slow injection of hypobaric bupivacaine solution (0.75% 6 mg, diluted to 2 ml with distilled water) at the rate of 1 ml/15 s through a 27-gauge needle. The patients maintained their position for 15 min before shifting to a supine position. In the other group levobupivacaine was used instead.

Results: Statistical study showed that the block characteristics were of no significant difference between the 2 groups (P>0.05) except that the duration of motor block was shorter in levobupivacaine group than in bupivacaine group (P<0.05). Blood pressure or heart rate showed no significant difference between the two groups during operation.

Conclusion: The block characteristics of hypobaric unilateral spinal block with levobupivacaine or bupivacaine are similar and their effect on circulatory function is slight. Levobupivacaine provides an option in case bupivacaine is not available.

Objective: To assess the value of Halena SAS-3 full automatic hemoglobin agarose electrophoresis in the diagnosis of thalassemia.

Methods: Hemoglobin A and A2 was detected by agarose electrophoresis in 200 blood samples collected from 170 healthy subjects, 2 patients with beta-thalassemia, 12 with acute lymphoblastic leukemia, 12 acute myelocytic leukemia and with 4 non-Hodgkin lymphoma.

Results: The Mean+/-SD of hemoglobin A in the healthy subjects and patients with beta-thalassemia, acute lymphoblastic leukemia, acute myelocytic leukemia and non-Hodgkin lymphoma was 97.55+/-0.51, 97.01+/-0.329, 97.42+/-0.57, 97.44+/-0.55, and that of A2 was 2.44+/-0.51, 2.99+/-0.32, 2.84+/-0.28, 2.55+/-0.55, respectively. The values of electrophoresed hemoglobin F of the 2 patients with beta-thalassemia were higher than those of the normal adults. The within-run coefficient of variation (CV) and between-run CV was 0.17 and 7.76 for hemoglobin A, and 0.16 and 7.14 for hemoglobin A2, respectively.

Conclusions: Halena SAS-3 full automatic agarose electrophoresis system possesses strong resolving power in detecting trace abnormal hemoglobin and accurately identifies unusual evaluation of hemoglobin A2 and F to facilitate the diagnosis of thalassemia.

Objective: To study the application of small thoracic incision for lobectomy with effects comparable to that of conventional incision.

Methods: A total of 386 patients with lung tumors were prospectively randomized into thoracic small incision group (n=184; average length of incision, 12.7 cm) and standard thoracic incision group (control group, n=152). The incision length, operation time, intra-operative bleeding volume, postoperative drainage volume, transfusion, hemostatic dosage, ambulation time, hospital stay and major complications were compared between the two groups.

Results: All of the indices in small incision group were significantly better than those of the control group (P<0.01) except the operation time (P>0.05). No severe complications occurred in small incision group, and in 4 cases conventional thoracotomy was performed instead due to adhesion or giant tumors.

Conclusions: Muscle-sparing thoracic small incision can achieve excellent clinical result and has such advantages as less invasiveness, reduced bleeding volume, pain and hospital stay, less complications, and better postoperative appearance. This procedure meets the requirements of lobectomy and provides an alternative for routine lobectomy for pulmonary tumors.

Objective: To study the pharmacodynamics of Jingjielianqiao decoction in promoting the healing of skin ulcer in mice.

Methods: Mouse models of skin ulcer were established by inducing skin trauma and scald, respectively. The skin ulcer of the mice was treated with Jingjielianqiao decoction or recombinant human epithelial growth factor (rhEGF), and the ulcer area after treatment was compared between the two groups and with their respective self control. Inflammatory cell infiltration in the ulcer was observed under the microscope.

Results: Compared with the self-control group, the skin ulcer area of mice treated with Jingjielianqiao decoction was reduced obviously (P<0.001), and the inflammatory cell infiltration also alleviated (P<0.001). Jingjielianqiao decoction produced significantly better therapeutic effect than rhEGF.

Conclusion: Jingjielianqiao decoction can obviously improve the healing of skin ulcer in mice with better effect than rhEGF.

Objective: To observe the effect of ambulatory labor analgesia on fetal oxygen saturation during labor.

Methods: Sixty parturients with uncomplicated term pregnancies were equally divided, according to their personal preference, into ambulatory analgesia group receiving combined spinal-epidural labor analgesia and control group without analgesia administration. All the parturients with ambulatory labor analgesia received sufentanil and ropivacaine administration. Fetal oxygen saturation was monitored continuously during the labor by recording the values every 5 minutes and the mean value was calculated. Umbilical blood was analyzed after fetal delivery and the pH, base excess (BE) value were recorded.

Results: In the analgesia group and the control group, the mean values of fetal oxygen saturation was (51.92+/-5.90)% and (50.77+/-6.47)%, respectively, during the first labor stage (P=0.48), and was (44.85+/-6.27)% and (43.12+/-7.34)% during the second labor stage (P=0.32), and the lowest values during the labor was (41.63+/-7.51)% and (40.96+/-7.48)%, respectively (P=0.73), showing no significant differences in the three values between the two groups.

Conclusion: Ambulatory labor analgesia does not significantly affect fetal oxygen saturation during the entire course of labor.